Protein Expression and Purification 
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This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.Comparison of physicochemical properties of recombinant buckwheat trypsin inhibitor (rBTI) and soybean trypsin inhibitor (SBTI)
Publication date: Available online 28 February 2020Source: Protein Expression and PurificationAuthor(s): Chen Li, Wenjie Li, Yi Zhang, Benjamin Kofi Simpson (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - February 29, 2020 Category: Biochemistry Source Type: research
Expression, purification, and characterization of recombinant apoPholasin
Publication date: Available online 28 February 2020Source: Protein Expression and PurificationAuthor(s): Satoshi Inouye, Yuiko Sahara-Miura, Mitsuhiro Nakamura, Takamitsu Hosoya (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - February 29, 2020 Category: Biochemistry Source Type: research
Expression and characterization of a chitinase from Serratia marcescens
Publication date: Available online 23 February 2020Source: Protein Expression and PurificationAuthor(s): Jincheng Li, Jiamin Zheng, Yanhui Liang, Renxiang Yan, Xinqi Xu, Juan LinAbstractA chitinase gene from Serratia marcescens was cloned and expressed in Escherichia coli and the properties of recombinant chitinase rCHI-2 were characterized. The optimum catalytic pH of rCHI-2 was 6.0. It was stable in the pH range of 6.0–9.0 and could maintain more than 90% of its relative enzyme activity after incubation at 37 °C for 1 h. The optimum catalytic temperature of the enzyme was 55 °C and 85% of enzyme activity was re...
Source: Protein Expression and Purification - February 24, 2020 Category: Biochemistry Source Type: research
A new insight into purification of polyphenol oxidase and inhibition effect of curcumin and quercetin on potato polyphenol oxidase
Publication date: Available online 22 February 2020Source: Protein Expression and PurificationAuthor(s): Mine AksoyAbstractIn the literature, the polyphenol oxidase (PPO) enzyme has been purified a many times via Sepharose 4B-l-tyrosine-p-aminobenzoic acid affinity column. In order to study PPO purification efficiency, 2-aminophenol and 4-aminophenol were applied as a spacer arm to CNBr-activated Sepharose 4B. The effects of the spacer arm on specific activity, purification fold, and electrophoretic properties were investigated. The best performance with 11.7-fold purification and 23951 U/mg protein specific activity was a...
Source: Protein Expression and Purification - February 24, 2020 Category: Biochemistry Source Type: research
Optimization of production conditions of rice α-galactosidase II displayed on yeast cell surface
Publication date: Available online 21 February 2020Source: Protein Expression and PurificationAuthor(s): Mosi Dong, Yun Gong, Jia Guo, Jing Ma, Suhong Li, Tuoping LiAbstractThe yeast surface displayed rice α-galactosidase II (YSD rice α-Gal II) was generated with the pYD1 vector. The expression and cultural conditions for the improvement of production of YSD rice α-Gal II were optimized. The results showed that several induction factors, which were the initial cell density, inoculation ratio, galactose (inducer) concentration, induction time and temperature, determined the activity and expression efficiency of YSD rice ...
Source: Protein Expression and Purification - February 23, 2020 Category: Biochemistry Source Type: research
An improved method for the expression and purification of porcine dihydropyrimidine dehydrogenase
Publication date: Available online 21 February 2020Source: Protein Expression and PurificationAuthor(s): Brett A. Beaupre, Joseph V. Roman, Graham R. MoranAbstractDihydropyrimidine dehydrogenase (DPD) catalyzes the reduction of uracil and thymine bases with electrons derived from NADPH. The mammalian DPD enzyme is a functional homodimer and has an elaborate cofactor arrangement. Two flavin cofactors (FAD and FMN) reside in two active site cavities that are separated by around 60 Å. The flavins are apparently bridged by four Fe4S4 clusters, two of which are provided by the partner protomer of the dimer. The study of DPD ...
Source: Protein Expression and Purification - February 23, 2020 Category: Biochemistry Source Type: research
Editorial Board
Publication date: May 2020Source: Protein Expression and Purification, Volume 169Author(s): (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - February 20, 2020 Category: Biochemistry Source Type: research
Starch-degrading enzymes from the brown-rot fungus Fomitopsis palustris
Publication date: Available online 16 February 2020Source: Protein Expression and PurificationAuthor(s): Yuki Tanaka, Naotake Konno, Tomohiro Suzuki, Naoto HabuAbstractBrown-rot fungi preferentially degrade softwood and cause severe breakdown of wooden structures. At the initial stage of the brown-rot decay, penetrating hyphae of the fungi are observed in ray parenchyma. Since starch grains are known to be present in the ray parenchyma of sapwood, investigation of the functions and roles of the starch-degrading enzymes is important to understand the initial stage of brown-rot decay. We purified and characterized two starch...
Source: Protein Expression and Purification - February 17, 2020 Category: Biochemistry Source Type: research
Affinity purification of Car9-tagged proteins on silica-derivatized spin columns and 96-well plates
Publication date: Available online 14 February 2020Source: Protein Expression and PurificationAuthor(s): Meng Xu, Matthew J. Bailey, Jennifer Look, Francois BaneyxAbstractThe Car9 affinity tag is a dodecameric silica-binding peptide that can be fused to the N- and C-termini of proteins of interest to enable their rapid and inexpensive purification on underivatized silica in a process that typically relies on l-lysine as an eluent. Here, we show that silica paper spin columns and borosilicate multi-well plates used for plasmid DNA purification are suitable for recovering Car9-tagged proteins with high purity in a workflow c...
Source: Protein Expression and Purification - February 15, 2020 Category: Biochemistry Source Type: research
Nanobody production can be simplified by direct secretion from Escherichia coli
Publication date: Available online 13 February 2020Source: Protein Expression and PurificationAuthor(s): Takayuki Iwaki, Kimiko Hara, Kazuo UmemuraAbstractIt is well known that camelids (camels and llamas) have fully functional antibodies with only a heavy chain consisting of a single variable domain and two constant domains. This single variable domain is called a “nanobody” and many nanobodies are synthesized in the cytosol of Escherichia coli, however, most of the nanobodies become inclusion bodies without tags to enhance their solubility. We generated a vector system to enable the secretary expression of nanobodies...
Source: Protein Expression and Purification - February 14, 2020 Category: Biochemistry Source Type: research
Initial development of biosimilar immune checkpoint blockers using HEK293 cells
Publication date: June 2020Source: Protein Expression and Purification, Volume 170Author(s): Michael Bernardes Ramos, Anna Erika Vieira de Araújo, Cristiane Pinheiro Pestana, Ana Paula Dinis Ano Bom, Renata Chagas Bastos, Aline de Almeida Oliveira, Patrícia Cristina da Costa Neves, Haroldo Cid da Silva JuniorAbstractAntibodies that block interaction of immune checkpoint receptors with its ligands have revolutionized the treatment of several cancers. Despite the success of this approach, the high cost has been restricted the use of this class of drugs. In this context, the development of biosimilar can be an important str...
Source: Protein Expression and Purification - February 14, 2020 Category: Biochemistry Source Type: research
Expression and purification of recombinant human serpin B1 yields novel molecules with altered protease inhibitory activities: Functional implications
Publication date: Available online 7 February 2020Source: Protein Expression and PurificationAuthor(s): Philip A. PembertonAbstractSerpin B1 regulates the innate immune system by inhibiting serine and cysteine proteases that control programmed cell death and proliferation pathways. To provide recombinant human proteins for in vitro and in vivo studies we expressed and purified wild-type human serpin B1 and a C344A variant in the yeast S. cerevisiae. Both proteins expressed well and inhibited elastase and chymotrypsin. However, purification of wild-type serpin B1 in the absence of a reducing agent resulted in the specific l...
Source: Protein Expression and Purification - February 8, 2020 Category: Biochemistry Source Type: research
Initial development of biosimilar immune checkpoint blockers using HEK293 cells
Publication date: Available online 7 February 2020Source: Protein Expression and PurificationAuthor(s): Michael Bernardes Ramos, Anna Erika Vieira Araújo, Cristiane Pinheiro Pestana, Ana Paula Dinis Ano Bom, Renata Chagas Bastos, Aline de Almeida Oliveira, Patrícia Cristina da Costa Neves, Haroldo Cid da Silva JuniorAbstractAntibodies that block interaction of immune checkpoint receptors with its ligands have revolutionized the treatment of several cancers. Despite the success of this approach, the high cost has been restricted the use of this class of drugs. In this context, the development of biosimilar can be an impor...
Source: Protein Expression and Purification - February 8, 2020 Category: Biochemistry Source Type: research
Bacterial expression and characterization of an anti-CD22 single-chain antibody fragment
Publication date: Available online 5 February 2020Source: Protein Expression and PurificationAuthor(s): Diana Mikiewicz, Natalia Łukasiewicz, Marcin Zieliński, Violetta Cecuda-Adamczewska, Anna Bierczyńska-Krzysik, Agnieszka Romanik-Chruścielewska, Małgorzata Kęsik-BrodackaAbstractSingle-chain variable fragment (scFv) antibodies are fusion proteins of the variable regions of the heavy and light chains of immunoglobulins connected with a short linker peptide. They possess unique and superior features compared to whole antibodies for immunotherapy of various carcinomas, including hematologic B-cell malignancies.In the ...
Source: Protein Expression and Purification - February 6, 2020 Category: Biochemistry Source Type: research
Molecular cloning, characterization, and heterologous expression of an acetyl-CoA acetyl transferase gene from Sanghuangporus baumii
Publication date: Available online 5 February 2020Source: Protein Expression and PurificationAuthor(s): Xutong Wang, Shixin Wang, Xinru Xu, Jian Sun, Yisha Ma, Zengcai Liu, Tingting Sun, Li ZouAbstractAcetyl-CoA C-acetyltransferase synthase gene (AACT) cDNA, DNA and promoter were cloned from Sanghuangporus baumii. The gene ORF (1260 bp) encoded 419 amino acids. The AACT DNA includes five exons (1–84 bp, 140–513 bp, 570–1027 bp, 1090–1282 bp, 1344–1494 bp) and four introns (85–139 bp, 514–569 bp, 1028–1089 bp, 1283–1343 bp). The molecular weight of AACT protein is 43.40 kDa, it is hydrophilic with a theo...
Source: Protein Expression and Purification - February 6, 2020 Category: Biochemistry Source Type: research
