Real-time PCR detection of mixed < i > Plasmodium ovale curtisi < /i > and < i > wallikeri < /i > infections in human and mosquito hosts
by Varun R. Potlapalli, Meredith S. Muller, Billy Ngasala, Innocent Mbulli Ali, Yu Bin Na, Danielle R. Williams, Oksana Kharabora, Srijana Chhetri, Mei S. Liu, Kelly Carey-Ewend, Feng-Chang Lin, Derrick Mathias, Brian B. Tarimo, Jonathan J. Juliano, Jonathan B. Parr, Jessica T. LinPlasmodium ovale curtisi (Poc) andPlasmodium ovale wallikeri (Pow) represent distinct non-recombiningPlasmodium species that are increasing in prevalence in sub-Saharan Africa. Though they circulate sympatrically, co-infection within human and mosquito hosts has rarely been described. Separate 18S rRNA real-time PCR assays that detectPoc andPow w...
Source: PLoS Neglected Tropical Diseases - December 8, 2023 Category: Tropical Medicine Authors: Varun R. Potlapalli Source Type: research
A Prospective cohort study of zoonotic cutaneous leishmaniasis in tunisia: Clinical and Immunological features and immune correlates of protection
ConclusionThis study on a large cohort at risk of ZCL confirmed IFN- γ and GrB as protective factors against the disease. A high IFN-γ/IL-10 ratio, but not GrB/IL-10 ratio was associated with resistance. These results are valuable for developing and evaluating of a vaccine against human leishmaniasis. (Source: PLoS Neglected Tropical Diseases)
Source: PLoS Neglected Tropical Diseases - December 8, 2023 Category: Tropical Medicine Authors: Ikbel Naouar Source Type: research
Screening of the Pandemic Response Box identifies anti-microsporidia compounds
by Qingyuan Huang, Jie Chen, Guoqing Pan, Aaron W. Reinke
Microsporidia are fungi obligate intracellular pathogens, which infect most animals and cause microsporidiosis. Despite the serious threat that microsporidia pose to humans and agricultural animals, few drugs are available for the treatment and control of microsporidia. To identify novel inhibitors, we took advantage of the model organismCaenorhabditis elegans infected with its natural microsporidianNematocida parisii. We used this system to screen the Pandemic Response Box, a collection of 400 diverse compounds with known antimicrobial activity. After testing thes...
Source: PLoS Neglected Tropical Diseases - December 8, 2023 Category: Tropical Medicine Authors: Qingyuan Huang Source Type: research
Prevalence of cystic echinococcosis in relatives of patients undergoing surgery for hepatic cystic echinococcosis in an endemic region
Conclusion/significanceThere is a high prevalence of CE in relatives of patients undergoing surgery by this disease in the province of Caut ín, Chile. (Source: PLoS Neglected Tropical Diseases)
Source: PLoS Neglected Tropical Diseases - December 8, 2023 Category: Tropical Medicine Authors: Carlos Manterola Source Type: research
< i > Mycobacterium ulcerans < /i > - < i > Bordetella trematum < /i > chronic tropical cutaneous ulcer: A four-case series, C ôte d’Ivoire
Conclusions/significanceIn four cases of Buruli ulcer in C ôte d’Ivoire,B.trematum was a co-pathogen which was resistant to rifampicin and clarithromycin, unmatchingM.ulcerans antibiotic susceptibility profile and counteracting the current treatment of Buruli ulcer in West Africa and Australia. Thus, we report here chronic mixedM.ulcerans-B.trematum chronic tropical ulcer as a specific form of Buruli ulcer in West Africa. (Source: PLoS Neglected Tropical Diseases)
Source: PLoS Neglected Tropical Diseases - December 7, 2023 Category: Tropical Medicine Authors: Bi Gor é Oscar Tchan Source Type: research
Public knowledge, belief, and preventive practices regarding dengue: Findings from a community-based survey in rural Bangladesh
ConclusionsThe findings of this study found a moderate level of knowledge regarding dengue among the community people. Despite having good practices in several aspects of dengue prevention among the participants, the overall score was not sufficient. This suggests that there is a pressing need for expanded education outreach aimed at increasing public awareness of dengue and encourage preventive practices within rural communities in Bangladesh. (Source: PLoS Neglected Tropical Diseases)
Source: PLoS Neglected Tropical Diseases - December 7, 2023 Category: Tropical Medicine Authors: Rajon Banik Source Type: research
The impact of the Sudan armed conflict on Mycetoma control
by Rawa Badri, Ahmed Hassan Fahal (Source: PLoS Neglected Tropical Diseases)
Source: PLoS Neglected Tropical Diseases - December 7, 2023 Category: Tropical Medicine Authors: Rawa Badri Source Type: research
Phylogeographic characterization of < i > Burkholderia pseudomallei < /i > isolated from Bangladesh
In this study, 22B.pseudomallei isolates, of which 20 clinical and two soil isolates were analyzed, utilizing Real-time PCR assay and multilocus sequence typing (MLST). The sequences were then submitted to PubMLST database for analysis and construction of phylogenetic tree. FindingsA total of 12 different sequence types (STs) that includes four novel STs were identified for the first time. Strains having STs 1005, 1007 and 56 were the most widespread STs frequently isolated in Bangladesh. ST 1005, ST 56, ST 1007 and ST 211 have been detected not only in Bangladesh but are also present in many Southeast Asian countries. Sig...
Source: PLoS Neglected Tropical Diseases - December 7, 2023 Category: Tropical Medicine Authors: Md. Shariful Alam Jilani Source Type: research
Excess mortality in northern Haiti during the 2010 cholera epidemic
In conclusion, addressing transportation and information needs could increase healthcare utilization and reduc e lives lost during an outbreak. (Source: PLoS Neglected Tropical Diseases)
Source: PLoS Neglected Tropical Diseases - December 6, 2023 Category: Tropical Medicine Authors: Macceau Medozile Source Type: research
Re-examination of the risk of dementia after dengue virus infection: A population-based cohort study
In conclusion, this study found de ngue patients had a slightly increased risk of non-vascular dementia and total dementia than those without dengue. However, the small corresponding E-values and sensitivity analyses suggest the association between dengue and dementia may not be causal. (Source: PLoS Neglected Tropical Diseases)
Source: PLoS Neglected Tropical Diseases - December 6, 2023 Category: Tropical Medicine Authors: Yu-Wen Chien Source Type: research
“It’s very saddening, you keep on wondering when the symptoms will be over”: A qualitative study exploring the long-term chikungunya disease impact on daily life and well-being, 6 years after disease onset
Conclusions/SignificanceThis study, the first of its kind, indicated that the adverse impact of long-term chikungunya disease is currently underreported. The persistent rheumatic symptoms had a negative effect on functional ability, which in turn impacted broad aspects of daily life and well-being, beyond what is captured by generic instruments. In the view of the findings, physical exercise programs including manual therapy, aerobics, resistance and stretching exercises, and orthopaedic footwear interventions in a multidisciplinary patient-centred approach may improve physical function and subsequently overall well-being....
Source: PLoS Neglected Tropical Diseases - December 6, 2023 Category: Tropical Medicine Authors: Churnalisa Doran Source Type: research
Differences in gene expression profiles in early and late stage rhodesiense HAT individuals in Malawi
by Peter Nambala, Julius Mulindwa, Harry Noyes, Vincent Pius Alibu, Barbara Nerima, Joyce Namulondo, Oscar Nyangiri, Enock Matovu, Annette MacLeod, Janelisa Musaya, on behalf of the TrypanoGEN+ Research Group as Members of the H3Africa ConsortiumT.b.rhodesiense is the causative agent of Rhodesian human African trypanosomiasis (r-HAT) in Malawi. Clinical presentation of r-HAT in Malawi varies between foci and differs from East African HAT clinical phenotypes. The purpose of this study was to gain more insights into the transcriptomic profiles of patients with early stage 1 and late stage 2 HAT disease in Malawi. Whole blood...
Source: PLoS Neglected Tropical Diseases - December 6, 2023 Category: Tropical Medicine Authors: Peter Nambala Source Type: research
Correction: Prevalence and intensity of soil-transmitted helminth infections in Uganda: Results from population-based prevalence surveys in five districts
by The Neglected Tropical Diseases Staff (Source: PLoS Neglected Tropical Diseases)
Source: PLoS Neglected Tropical Diseases - December 6, 2023 Category: Tropical Medicine Authors: The Neglected Tropical Diseases Staff Source Type: research
Economic burden of dengue in urban Bangladesh: A societal perspective
ConclusionsDengue disease imposes a substantial financial burden on households and society. Therefore, decision-makers should consider the treatment cost of dengue infections, particularly among the poor in the population while balancing the benefits of introducing potentially effective dengue preventive programs in Bangladesh. (Source: PLoS Neglected Tropical Diseases)
Source: PLoS Neglected Tropical Diseases - December 5, 2023 Category: Tropical Medicine Authors: Abdur Razzaque Sarker Source Type: research
< i > Salmonella typhimurium < /i > targeting with monoclonal antibodies prevents infection in mice
This study aims to prepare monoclonal antibodies (Mabs) with broad reactivity against multi-serotypeSalmonella strains, potentially offering cross-protection. We prepared two Mabs F1D4 and B7D4 against protein FliK and BcsZ, two potential vaccine candidates against multi-serotypeSalmonella. The two Mabs belonging to IgG1 isotype exhibited high titers of 1:256,000 and 1:512,000 respectively, as well as broad cross-reactivity against 28 different serotypes ofSalmonella strains with percentages of 89.29% and 92.86%, correspondingly. Neutralizing effects of the two Mabs onSalmonella growth, adhesion, invasion and motility was ...
Source: PLoS Neglected Tropical Diseases - December 4, 2023 Category: Tropical Medicine Authors: Jie Li Source Type: research
