Editorial Board
Publication date: July 2020Source: Plasmid, Volume 110Author(s): (Source: Plasmid)
Source: Plasmid - July 7, 2020 Category: Biotechnology Source Type: research

Construction of a novel CRISPRi-based tool for silencing of multiple genes in Mycobacterium tuberculosis
Publication date: Available online 11 June 2020Source: PlasmidAuthor(s): Nisheeth Agarwal (Source: Plasmid)
Source: Plasmid - June 13, 2020 Category: Biotechnology Source Type: research

Quantifying plasmid dynamics using single-cell microfluidics and image bioinformatics
Publication date: Available online 11 June 2020Source: PlasmidAuthor(s): J.C.R. Hernandez-Beltran, J. Rodríguez-Beltrán, A. San Millán, R. Peña-Miller, A. Fuentes-Hernández (Source: Plasmid)
Source: Plasmid - June 13, 2020 Category: Biotechnology Source Type: research

The ever-expanding tcp conjugation locus of pCW3 from Clostridium perfringens
Publication date: Available online 9 June 2020Source: PlasmidAuthor(s): Sarah A. Revitt-Mills, Thomas D. Watts, Dena Lyras, Vicki Adams, Julian I. Rood (Source: Plasmid)
Source: Plasmid - June 10, 2020 Category: Biotechnology Source Type: research

Construction of a novel dual-inducible duet-expression system for gene (over)expression in Pseudomonas putida
Publication date: Available online 3 June 2020Source: PlasmidAuthor(s): Rahul Gauttam, Aindrila Mukhopadhyay, Steven W. Singer (Source: Plasmid)
Source: Plasmid - June 4, 2020 Category: Biotechnology Source Type: research

The cell-penetrating YopM protein-functionalized quantum dot-plasmid DNA conjugate as a novel gene delivery vector
Publication date: Available online 2 June 2020Source: PlasmidAuthor(s): Özge Uğurlu, Fırat Barış Barlas, Serap Evran, Suna Timur (Source: Plasmid)
Source: Plasmid - June 4, 2020 Category: Biotechnology Source Type: research

Plasmidome of an environmental Acinetobacter lwoffii strain originating from a former gold and arsenic mine
Publication date: Available online 4 May 2020Source: PlasmidAuthor(s): Tomasz Walter, Joanna Klim, Marcin Jurkowski, Iwona Köhling, Małgorzata Słodownik, Urszula Zielenkiewicz (Source: Plasmid)
Source: Plasmid - May 5, 2020 Category: Biotechnology Source Type: research

Editorial Board
Publication date: May 2020Source: Plasmid, Volume 109Author(s): (Source: Plasmid)
Source: Plasmid - April 25, 2020 Category: Biotechnology Source Type: research

Formation of new PHE plasmids in pseudomonads in a phenol-polluted environment
Publication date: Available online 11 April 2020Source: PlasmidAuthor(s): Eve Elken, Eeva Heinaru, Merike Jõesaar, Ain Heinaru (Source: Plasmid)
Source: Plasmid - April 12, 2020 Category: Biotechnology Source Type: research

Incompatibility and phylogenetic relationship of I-complex plasmids
Publication date: May 2020Source: Plasmid, Volume 109Author(s): Marta Rozwandowicz, Joost Hordijk, Alex Bossers, Aldert L. Zomer, Jaap A. Wagenaar, Dik J. Mevius, Michael S.M. Brouwer (Source: Plasmid)
Source: Plasmid - March 29, 2020 Category: Biotechnology Source Type: research

Tailor-made sRNAs: A plasmid tool to control the expression of target mRNAs in Pseudomonas putida
Publication date: Available online 21 March 2020Source: PlasmidAuthor(s): Patrícia Apura, Margarida Saramago, Alexandra Peregrina, Sandra C. Viegas, Sandra M. Carvalho, Lígia M. Saraiva, Cecília M. Arraiano, Susana Domingues (Source: Plasmid)
Source: Plasmid - March 23, 2020 Category: Biotechnology Source Type: research

Editorial Board
Publication date: March 2020Source: Plasmid, Volume 108Author(s): (Source: Plasmid)
Source: Plasmid - February 13, 2020 Category: Biotechnology Source Type: research

Strong synthetic stationary phase promoter-based gene expression system for Escherichia coli
Publication date: Available online 5 February 2020Source: PlasmidAuthor(s): Jananee Jaishankar, Preeti SrivastavaAbstractThe Gram-negative bacterium Escherichia coli has been the work horse for recombinant protein production since the past several years. However, most of the gene expression systems used either require expensive inducers or exhibit low strength. In the present study, we have generated a strong promoter by repeated rounds of random mutagenesis in a stationary phase promoter isolated from Gordonia sp. IITR100. The promoter activity increased 16-fold as compared to the wild-type promoter. The resultant synthet...
Source: Plasmid - February 7, 2020 Category: Biotechnology Source Type: research

Cloning of pAhX22, a small cryptic plasmid from Aeromonas hydrophila, and construction of a pAhX22-derived shuttle vector
In this study, a cryptic plasmid from Aeromonas hydrophila (pAhX22) was cloned and characterized. pAhX22 was 2523 bp long, had a GC content of 59.9%, and contained two putative open reading frames (ORFs). orf1 and orf2 encoded putative proteins of 458 amino acids and 88 amino acids, respectively; these putative proteins might be involved in plasmid replication. An Escherichia coli–A. hydrophila shuttle vector, pAEsv-1 (4587 bp, KanR), was constructed using in-fusion cloning, combining pAhX22 with the kanamycin-resistance gene and the origin of replication from E. coli expression vector pET-28a. The transformation...
Source: Plasmid - January 30, 2020 Category: Biotechnology Source Type: research

The role of hosts, plasmids and environment in determining plasmid transfer rates: A meta-analysis
Publication date: Available online 10 January 2020Source: PlasmidAuthor(s): Richard J. Sheppard, Alice Beddis, Timothy G. BarracloughAbstractPlasmids transfer at highly variable rates which spread over 10 orders of magnitude. While rates have been measured for decades and it is known that the rates are affected by biotic and abiotic factors, it is unclear how and to what extent these factors determine the rates of transfer. We performed a meta-analysis of 1224 published transfer rates from 33 papers (filtered to 612 transfer rates) to assess this variation. Over three quarters of the variation can be predicted, with plasmi...
Source: Plasmid - January 11, 2020 Category: Biotechnology Source Type: research

ORF-based binarized structure network analysis of plasmids (OSNAp), a novel approach to core gene-independent plasmid phylogeny
ConclusionOSNAp is a novel holistic approach to assess relatedness of a population of plasmids in a given plasmid group based on nucleotide sequence data. It provides higher discrimination than pMLST, which may prove useful in tracing bacteria that harbor plasmids of shared origins. (Source: Plasmid)
Source: Plasmid - December 21, 2019 Category: Biotechnology Source Type: research

Editorial Board
Publication date: January 2020Source: Plasmid, Volume 107Author(s): (Source: Plasmid)
Source: Plasmid - December 20, 2019 Category: Biotechnology Source Type: research

A novel expression vector for Corynebacterium glutamicum with an auxotrophy complementation system
In this study, the novel Escherichia coli-C. glutamicum shuttle expression vector pLY-4, derived from the expression vector pXMJ19, was constructed. The constitutive vector pLY-4 contains a large multiple cloning site, the strong promoter tacM and two selective markers: the original chloramphenicol resistance gene cat is used for molecular cloning operations, and the auxotrophy complementation marker alr, which can be stably replicated in the auxotrophic host strain without antibiotic selection pressure, is used for industrial fermentation. Heterologous expression of the gapC gene using the vector pLY-4 in C. glutamicum fo...
Source: Plasmid - November 21, 2019 Category: Biotechnology Source Type: research

Editorial Board
Publication date: November 2019Source: Plasmid, Volume 106Author(s): (Source: Plasmid)
Source: Plasmid - November 12, 2019 Category: Biotechnology Source Type: research

The Tcp plasmids of Clostridium perfringens require the resP gene to ensure stable inheritance
Publication date: Available online 9 November 2019Source: PlasmidAuthor(s): Sarah Revitt-Mills, Carmen Lao, Marie Archambault, Dena Lyras, Julian I. Rood, Vicki AdamsAbstractMany of the disease-causing toxins of the pathogenic bacterium Clostridium perfringens are harboured on large, highly stable, conjugative plasmids. Previous work has established the requirement of a ParMRC-like partitioning system for plasmid maintenance, but little is known about other mechanisms used to ensure stable plasmid inheritance. The archetypal 47 kb Tcp plasmid, pCW3, encodes a gene, resP, whose putative product has sequence similarity to ...
Source: Plasmid - November 10, 2019 Category: Biotechnology Source Type: research

Corrigendum to “De novo synthesis of the complete genome of coxsackievirus A10 based on Golden Gate cloning combined with promoter reconstruction” [Plasmid 103 (2019) 17–24]
Publication date: Available online 9 November 2019Source: PlasmidAuthor(s): Meng Li, Jing Chen (Source: Plasmid)
Source: Plasmid - November 9, 2019 Category: Biotechnology Source Type: research

SGI0, a relative of Salmonella genomic islands SGI1 and SGI2, lacking a class 1 integron, found in Proteus mirabilis
Publication date: Available online 6 November 2019Source: PlasmidAuthor(s): Claire de Curraize, Eliane Siebor, Catherine Neuwirth, Ruth M. HallAbstractSeveral groups of integrative mobilizable elements (IMEs) have been found at the 3′-end of the chromosomal trmE gene, all harbouring a class 1 integron. Here, a new IME, Salmonella Genomic Island 0 (SGI0), was found in trmE in a French clinical Proteus mirabilis strain. SGI0 was characterized by the lack of class 1 integron. SGI0 is closely related to the backbones of SGI1 (97.59% nucleotide identity) and SGI2 (97.72% nucleotide identity). SGI0 harboured also the AcaCD...
Source: Plasmid - November 7, 2019 Category: Biotechnology Source Type: research

Plasmid pSfr64a and the symbiotic plasmid pSfr64b of Sinorhizobium fredii GR64 control each other's conjugative transfer through quorum-sensing elements
Publication date: Available online 2 November 2019Source: PlasmidAuthor(s): Laura Cervantes, Fabiola Miranda-Sánchez, Gonzalo Torres Tejerizo, David Romero, Susana BromAbstractRhizobia are nitrogen-fixing symbionts of plants. Their genomes frequently contain large plasmids, some of which are able to perform conjugative transfer. Plasmid pSfr64a from Sinorhizobium fredii GR64 is a conjugative plasmid, whose transfer is regulated by quorum sensing genes encoded by itself (traR64a, traI64a), in the symbiotic plasmid pSfr64b (traR64b, traI64b), and in the chromosome (ngrI). Also, transfer of pSfr64b requires quorum sens...
Source: Plasmid - November 3, 2019 Category: Biotechnology Source Type: research

Two novel transcriptional reporter systems for monitoring Helicobacter pylori stress responses
Publication date: November 2019Source: Plasmid, Volume 106Author(s): A.M. Belova, D.V. Basmanov, V.V. Babenko, O.V. Podgorny, T.V. Mitko, K.A. Prusakov, D.V. Klinov, V.N. LazarevAbstractHelicobacter pylori, a human pathogen linked to many stomach diseases, is well adapted to colonize aggressive gastric environments, and its virulence factors contribute this adaptation. Here, we report the construction of two novel H. pylori vectors, pSv2 and pSv4, carrying a reporter gene fused to the promoters of virulence factor genes for monitoring the response of single H. pylori cells to various stresses. H. pylori cryptic plasmids we...
Source: Plasmid - November 3, 2019 Category: Biotechnology Source Type: research

Synthetic promoter for efficient and muscle-specific expression of exogenous genes
Publication date: Available online 30 October 2019Source: PlasmidAuthor(s): Yili Liu, Yutong He, Yong Wang, Ming Liu, Mingfeng Jiang, Rong Gao, Gang WangAbstractSynthetic promoters (SPs) have many advantages over their natural counterparts, especially with regard to transcriptional activity and tissue specificity. Here, we report a new strategy to construct SPs for efficient and muscle-specific gene expression. First, 19 nucleic acid motifs classified to 3 kinds of transcriptional regulatory elements were rationally selected. A recombinant promoter library was constructed by randomly assembling these motifs. Second, the tr...
Source: Plasmid - October 31, 2019 Category: Biotechnology Source Type: research

Dissemination of blaKPC-2 in an NTEKPC by an IncX5 plasmid
This study reports the dissemination of a blaKPC-2-carrying NTEKPC among ST11/CG258 K. pneumoniae strains and ST1642 K. quasipneumoniae subsp. quasipneumoniae AMKP9 in an Amazonian hospital. The dissemination was due to pAMKP10, an ~48 kbp IncX5 plasmid carrying ΔISKpn6/blaKPC-2/ISKpn27 in a Tn1722-based unit. Although similar to NTEKPC-Ia from pKP048 described in China, a different transposase is present upstream of ISKpn27. Additionally, mutations were identified downstream of ISKpn27 but did not affect the blaKPC-2 promoter regions. pAMKP10 conjugated in vitro only from CG258 isolates. Since CG258 strains are gene...
Source: Plasmid - October 26, 2019 Category: Biotechnology Source Type: research

Editorial Board
Publication date: September 2019Source: Plasmid, Volume 105Author(s): (Source: Plasmid)
Source: Plasmid - October 26, 2019 Category: Biotechnology Source Type: research

Seamless insert-plasmid assembly at sub-terminal homologous sequences
Publication date: Available online 24 October 2019Source: PlasmidAuthor(s): Anna-Sophia Krebs, Tobias Bierig, Gabriella Collu, Roger M. BenoitAbstractThe engineering of fusion proteins for structural biology and protein nanotechnology often requires seamless DNA assembly with slight variations in the domain boundaries. To improve the molecular biology workflow for such projects, we evaluated the use of sub-terminal homologous sequences (HS) for co-transformation cloning and for T5 exonuclease / Phusion DNA polymerase mediated in vitro assembly. To quantify the effects of different HS-to-ends distances on cloning efficiency...
Source: Plasmid - October 26, 2019 Category: Biotechnology Source Type: research

The biology of IncI2 plasmids shown by whole-plasmid multi-locus sequence typing
Publication date: Available online 17 October 2019Source: PlasmidAuthor(s): Richard J. MeinersmannAbstractIncI2 type plasmids are medium-sized (~55–80 kb) conjugative plasmids that have been found carrying important antimicrobial resistance genes but have also been frequently found as cryptic plasmids. The DNA sequences for 147 fully sequenced IncI2 plasmids were studied by a whole-plasmid multi-locus sequence typing (wpMLST) scheme. A total of 171 loci were identified of which 52 were considered core (carried by greater than 95% of the plasmids). Most of the plasmids carrying the antimicrobial gene mcr-1 were in a...
Source: Plasmid - October 19, 2019 Category: Biotechnology Source Type: research

Identification of a novel conjugative plasmid carrying the multiresistance gene cfr in Proteus vulgaris isolated from swine origin in China
In this study, a novel conjugative plasmid carrying cfr, designated as pPvSC3, was characterized in a Proteus vulgaris strain isolated from swine in China. Plasmid pPvSC3 is 284,528 bp in size and harbors 10 other antimicrobial resistance genes, making it a novel plasmid that differs from all known plasmids due to its unique backbone and repA gene. BLAST analysis of the plasmid sequence shows no significant homology to any known plasmid backbone, but shows high level homology to Providencia rettgeri strain CCBH11880 Contig_9, a strain isolated from surgical wound in Brazil, 2014. There are two resistance-determining regi...
Source: Plasmid - September 7, 2019 Category: Biotechnology Source Type: research

Editorial
Publication date: Available online 28 August 2019Source: PlasmidAuthor(s): Eva M. Top, Beth Traxler, Julian I. Rood (Source: Plasmid)
Source: Plasmid - August 29, 2019 Category: Biotechnology Source Type: research

Inside or outside? A new collection of Gateway vectors allowing plant protein subcellular localization or over-expression
Publication date: Available online 23 August 2019Source: PlasmidAuthor(s): François Berthold, David Roujol, Caroline Hemmer, Elisabeth Jamet, Christophe Ritzenthaler, Laurent Hoffmann, Corinne Schmitt-KeichingerAbstractTransient expression of proteins based on agro-infiltration techniques has proven very efficient and straightforward to study the intrinsic properties of proteins. The level of protein expression has been enhanced by the use of vector plasmids containing virus-derived sequences and the cloning step has been facilitated by recombination technologies. The pEAQ-HT-DEST series of vectors fulfilling these ...
Source: Plasmid - August 25, 2019 Category: Biotechnology Source Type: research

Transformation of the rhizospheric Bacillus cereus sensu lato B25 strain using a room-temperature electrocompetent cells preparation protocol
Publication date: Available online 30 July 2019Source: PlasmidAuthor(s): Estefanía Morales-Ruiz, Astrid López Ceballos, Ignacio E. Maldonado-MendozaAbstractBacterial transformation is a crucial step in the genetic manipulation of a bacterium. However, Gram-positive bacteria are difficult to transform and consequently many different methodologies have been developed. Here, we examined the transformation efficiencies of an electroporation protocol by varying three main factors: the composition of the electroporation buffer, the strength of the electric pulse, and the composition of the recovery media. Overall, ...
Source: Plasmid - July 31, 2019 Category: Biotechnology Source Type: research

Editorial Board
Publication date: July 2019Source: Plasmid, Volume 104Author(s): (Source: Plasmid)
Source: Plasmid - July 27, 2019 Category: Biotechnology Source Type: research

Publisher's Note
Publication date: July 2019Source: Plasmid, Volume 104Author(s): (Source: Plasmid)
Source: Plasmid - July 27, 2019 Category: Biotechnology Source Type: research

B/O plasmid R16 from 1956 carries an In1-like class 1 integron embedded in a complex region containing parts of the Acinetobacter baumannii AbaR resistance island
Publication date: Available online 18 July 2019Source: PlasmidAuthor(s): Robert A. Moran, Ruth M. HallAbstractThe IncB/O multiresistance plasmid R16, recovered in 1956 and used in experimental studies of B/O plasmid features, was sequenced. The resistance genes are all within a class 1 integron closely related to In1 containing the cassette array oxa2-aadA1-oxa2-orfD and sul1 in the 3′-conserved segment (3′-CS), with Tn10, containing tetA(B), inserted just inside the 3′-CS. The integron is part of a 25,144 bp region inserted in the plasmid backbone, bounded on only one side by a truncated Tn6018 and fla...
Source: Plasmid - July 18, 2019 Category: Biotechnology Source Type: research

Improving biosynthetic production of pinene through plasmid recombination elimination and pathway optimization
Publication date: Available online 15 July 2019Source: PlasmidAuthor(s): Shao-Heng Bao, Dong-Yi Zhang, Er MengAbstractPinene is a monoterpene with wide industrial applications, especially as a promising high energy-density jet fuel. Traditional production of pinene on an industrial scale is material consumptive and has a low yield. As an alternative, microbial organisms have been engineered though advanced synthetic biological techniques to produce a variety of heterologous products, including pinene. Here, we investigated the stability of genetic circuits encoding the pinene producing pathway during fermentation and its r...
Source: Plasmid - July 16, 2019 Category: Biotechnology Source Type: research

The piggyBac-based double-inducible binary vector system: A novel universal platform for studying gene functions and interactions
Publication date: Available online 29 June 2019Source: PlasmidAuthor(s): Wencui Sun, Jiawen Teng, Jiahui Zeng, Yuan Xue, Jing Chang, Yonggang Zhang, Xu Pan, Ya Zhou, Mowen Lai, Guohui Bian, Qiongxiu Zhou, Jiaxin Liu, Bo Chen, Feng MaAbstractEukaryotic inducible overexpression systems, including Tet-On and mifepristone-inducible systems, have been widely used to study gene functions by reverse genetics. Among the transposon systems reported to date, the piggyBac transposon system is one of the most efficient in cultured mammalian cells. Here, we report a piggyBac-based double-inducible system that combined the advantages of...
Source: Plasmid - June 30, 2019 Category: Biotechnology Source Type: research

Assembly of large mobilizable genetic cargo by double recombinase operated insertion of DNA (DROID)
Publication date: Available online 25 June 2019Source: PlasmidAuthor(s): Kevin Neil, Nancy Allard, David Jordan, Sébastien RodrigueAbstractThere is an important need to develop new therapeutic tools to modulate the gene content of microbiomes. A potential strategy for microbiome engineering relies on the delivery of genetic payloads by conjugative plasmids. Yet, the introduction of large DNA molecules in conjugative plasmids can be challenging. Here, we describe the Double Recombinase Operated Insertion of DNA (DROID), an efficient method to assemble large DNA molecules without introducing antibiotic resistance gene...
Source: Plasmid - June 25, 2019 Category: Biotechnology Source Type: research

A phylogenetic test of the role of CRISPR-Cas in limiting plasmid acquisition and prophage integration in bacteria
Publication date: Available online 11 June 2019Source: PlasmidAuthor(s): Derek O'Meara, Leonard NunneyAbstractCRISPR-Cas is a prokaryotic defense system capable of protecting the cell from damaging foreign genetic elements. However, some genetic elements can be beneficial, which suggests the hypothesis that bacteria with CRISPR-Cas incur a cost of reduced intake of mutualistic plasmids and prophage. Here we present the first robust test of this hypothesis that controls for phylogenic and ecological biases in the distribution of CRISPR-Cas. We filtered the available genomic data (~7000 strains from ~2100 species) by first s...
Source: Plasmid - June 12, 2019 Category: Biotechnology Source Type: research

Plasmid analysis of Escherichia coli isolates from South Korea co-producing NDM-5 and OXA-181 carbapenemases
Publication date: Available online 28 May 2019Source: PlasmidAuthor(s): Jin Yang Baek, Sun Young Cho, So Hyun Kim, Cheol-In Kang, Kyong Ran Peck, Jae-Hoon Song, Doo Ryeon Chung, Kwan Soo KoAbstractRecently, Escherichia coli isolates co-producing New Delhi metallo-β-lactamase (NDM)-5 and oxacillinase (OXA)-181 were identified in a tertiary-care hospital of South Korea. Isolate CC1702–1 was collected from urine in January 2017 and isolate CC1706-1 was recovered from a transtracheal aspirate of a hospitalized patient in May 2017. Carbapenemase genes were identified by multiplex PCR and sequencing, and whole genome ...
Source: Plasmid - May 30, 2019 Category: Biotechnology Source Type: research

Editorial Board
Publication date: May 2019Source: Plasmid, Volume 103Author(s): (Source: Plasmid)
Source: Plasmid - May 18, 2019 Category: Biotechnology Source Type: research

The secret life of conjugative relaxases
Publication date: Available online 17 May 2019Source: PlasmidAuthor(s): Dolores Lucía Guzmán-Herrador, Matxalen LlosaAbstractConjugative relaxases are well-characterized proteins responsible for the site- and strand-specific endonucleolytic cleavage and strand transfer reactions taking place at the start and end of the conjugative DNA transfer process. Most of the relaxases characterized biochemically and structurally belong to the HUH family of endonucleases. However, an increasing number of new families of relaxases are revealing a variety of protein folds and catalytic alternatives to accomplish conjugativ...
Source: Plasmid - May 18, 2019 Category: Biotechnology Source Type: research

Delineation of the integrase-attachment and origin-of-transfer regions of the symbiosis island ICEMlSymR7A
Publication date: Available online 9 May 2019Source: PlasmidAuthor(s): Callum J. Verdonk, John T. Sullivan, Kate M. Williman, Leila Nicholson, Tahlia R. Bastholm, Michael F. Hynes, Clive W. Ronson, Charles S. Bond, Joshua P. RamsayAbstractIntegrative and conjugative elements (ICEs) are chromosomally-integrated mobile genetic elements that excise from their host chromosome and transfer to other bacteria via conjugation. ICEMlSymR7A is the prototypical member of a large family of “symbiosis ICEs” which confer upon their hosts the ability to form a nitrogen-fixing symbiosis with a variety of legume species. Mesorh...
Source: Plasmid - May 10, 2019 Category: Biotechnology Source Type: research

Escherichia coli vectors having stringently repressible replication origins allow a streamlining of Crispr/Cas9 gene editing
This study describes the construction of an expanded series of plasmid vectors having replication origins of p15a, RSF1030 or RSF1031 that like pAM34 have IPTG-dependent replication. Surprisingly, these plasmids can be cured in fewer generations than pAM34. Derivatives of pAM34 with alternative antibiotic selection markers were also constructed. The utility of these vectors is demonstrated in the construction of a CRISPR-Cas9 system consisting of an IPTG-dependent Cas9 plasmid and a curable guide RNA plasmid having a streptomycin counterselection marker. This system was successfully demonstrated by construction of point mu...
Source: Plasmid - May 1, 2019 Category: Biotechnology Source Type: research

Molecular and genetic characterization of the pOV plasmid from Pasteurella multocida and construction of an integration vector for Gallibacterium anatis
ConclusionThe pOV plasmid is a wide-range, low-copy-number plasmid that is able to replicate in some gamma-proteobacteria. Part of this plasmid was integrated into the G. anatis 12656-12 chromosome. This construct may prove to be a useful tool for genetic studies of G. anatis. (Source: Plasmid)
Source: Plasmid - April 24, 2019 Category: Biotechnology Source Type: research

Mobilisation of a small Acinetobacter plasmid carrying an oriT transfer origin by conjugative RepAci6 plasmids
Publication date: Available online 11 April 2019Source: PlasmidAuthor(s): Grace A. Blackwell, Ruth M. HallAbstractMost Acinetobacter plasmids are genus specific but their properties have not been investigated. Small plasmids with Rep_3 family replication initiation proteins and iterons are common in Acinetobacter baumannii and often carry antibiotic resistance genes and toxin-antitoxin systems. A RepAci1 plasmid, carrying the carbapenem resistance gene oxa23 in Tn2006 and a RepAci2 plasmid carrying the amikacin (kanamycin and neomycin) resistance gene aphA6 in TnaphA6 were identified. These two plasmids have related rep re...
Source: Plasmid - April 13, 2019 Category: Biotechnology Source Type: research

Editorial Board
Publication date: March 2019Source: Plasmid, Volume 102Author(s): (Source: Plasmid)
Source: Plasmid - April 12, 2019 Category: Biotechnology Source Type: research

A simple dual-inducible CRISPR interference system for multiple gene targeting in Corynebacterium glutamicum
Publication date: Available online 4 April 2019Source: PlasmidAuthor(s): Rahul Gauttam, Gerd M. Seibold, Phillipp Mueller, Tatjana Weil, Tamara Weiß, René Handrick, Bernhard J. EikmannsAbstractThe development of CRISPR interference (CRISPRi) technology has dramatically increased the pace and the precision of target identification during platform strain development. In order to develop a simple, reliable, and dual-inducible CRISPRi system for the industrially relevant Corynebacterium glutamicum, we combined two different inducible repressor systems in a single plasmid to separately regulate the expression of dC...
Source: Plasmid - April 5, 2019 Category: Biotechnology Source Type: research

De novo synthesis of the complete genome of coxsackievirus A10 based on Golden Gate cloning combined with promoter reconstruction
Publication date: Available online 27 March 2019Source: PlasmidAuthor(s): Meng Li, Xinguo Li, Xiaoqi Chen, Shuo ShenAbstractIn the present study, a donor plasmid derived from pUC19 and two recipient plasmids, which had been modified from the donor plasmid and contained the red fluorescence protein gene mCherry as a reporter gene downstream of the hybrid tac promoter with the −35 region deletion mutation, were constructed. The complete genome sequence of coxsackievirus A10 downstream of a T7 promoter was divided into 7 fragments and synthesized with overlap extension PCR and the DNAworks program. Then, using the Golde...
Source: Plasmid - March 28, 2019 Category: Biotechnology Source Type: research