Metabolic Alterations in Streptozotocin –nicotinamide-induced Diabetic Rats Treated with Muntingia calabura Extract via 1H-NMR-based
Metabolomics
This study aims to support the traditional claim of MC as a functional food and blood-glucose-lowering regimen. The antidiabetic potential of MC is tested on a
streptozotocin–nicotinamide (STZ-NA)-induced diabetic rat model by using the 1H-NMR-based metabolomic approach. Serum biochemical analyses reveal that treatment with 250 mg/kg
body weight (bw) standardized freeze-dried (FD) 50% ethanolic MC extract (MCE 250) shows favorable serum creatinine (37.77 ± 3.53 µM), urea (5.98 ± 0.84 mM) and glucose (7.36 ± 0.57 mM)
lowering capacity, which was comparable to the standard drug, metfo...
Source: Planta Medica - May 15, 2023 Category: Drugs & Pharmacology Authors: Zolkeflee, Nur Khaleeda Zulaikha Wong, Pei Lou Maulidiani, M. Ramli, Nurul Shazini Azlan, Azrina Abas, Faridah Tags: Original Papers Source Type: research
Overexpression and RNAi-mediated Knockdown of Two 3 β-hydroxy-Δ5-steroid dehydrogenase Genes in Digitalis lanata Shoot Cultures Reveal Their Role in Cardenolide
Biosynthesis
Planta Med DOI: 10.1055/a-2074-91863β-hydroxy-Δ5-steroid dehydrogenases (3βHSDs) are supposed to be involved in 5β-cardenolide biosynthesis. Here, a novel 3βHSD (Dl3βHSD2) was isolated from Digitalis
lanata shoot cultures and expressed in E. coli. Recombinant Dl3βHSD1 and Dl3βHSD2 shared 70% amino acid identity, reduced various 3-oxopregnanes and oxidised
3-hydroxypregnanes, but only rDl3βHSD2 converted small ketones and secondary alcohols efficiently. To explain these differences in substrate specificity, we established homology
models using borneol dehydrogenase of Salvia rosmarinus (6zyz) as the ...
Source: Planta Medica - May 15, 2023 Category: Drugs & Pharmacology Authors: Leykauf, Tim Klein, Jan Ernst, Mona Dorfner, Maja Ignatova, Anastasiia Kreis, Wolfgang Lanig, Harald Munkert, Jennifer Tags: Original Papers Source Type: research
Bio-evaluation of Untapped Alkaloids from Vinca minor Enriched by Methyl-jasmonate-induced Stress: an Integrated Approach
In this study, three compounds identified as 9-methoxyvincamine, minovincinine, and minovincine are successfully isolated in good yield
and subjected to several bioassays based on a network pharmacology study. The extracts and isolated compounds show weak to moderate antimicrobial and cytotoxic activities. Also, they are
found to significantly promote wound healing in scratch assay, and transforming growth factor-β (TGF-β) modulation is suggested to be the potential pathway based on
bioinformatic analysis. Hence, Western blotting is used to assess the expression of several markers related to this pathway a...
Source: Planta Medica - May 15, 2023 Category: Drugs & Pharmacology Authors: El-Senduny, Fardous F. Elgazar, Abdullah A. Alwasify, Heba Allah Abed, Alaa Foda, Mohamed Abouzeid, Sara Lewerenz, Laura Selmar, Dirk Badria, Farid Tags: Original Papers Source Type: research
Indole Alkaloids of Rauvolfia ligustrina and Their Anxiolytic Effects in Adult Zebrafish
Planta Med DOI: 10.1055/a-2058-3710
Rauvolfia species are well known as producers of bioactive monoterpene indole alkaloids, which exhibit a broad spectrum of biological activities. A new vobasine-sarpagan-type
bisindole alkaloid (1) along with six known monomeric indoles (2, 3/4, 5, and 6/7) were isolated from the ethanol extract of the roots of Rauvolfia ligustrina.
The structure of the new compound was elucidated by interpretation of their spectroscopic data (1D and 2D NMR and HRESIMS) and comparison with published data for analog compounds. The
cytotoxicity of the isolated compounds was screened i...
Source: Planta Medica - May 15, 2023 Category: Drugs & Pharmacology Authors: de Sousa, Herbert Magalh ães da Silva, Alison Batista Ferreira, Maria Kueirislene Am âncio da Silva, Antonio Wlisses de Menezes, Jane Eire Silva Alencar Marinho, Emmanuel Silva Marinho, M árcia Machado dos Santos, Helcio Silva Pessoa, Otilia Deusd êni Tags: Original Papers Source Type: research
Structural Characterization and Anti-inflammatory Activities of Anticomplementary Polysaccharides from Rhododendron principis
Planta Med DOI: 10.1055/a-2063-5595
Rhododendron principis leaves have been used as “Dama”, a Traditional Tibetan Medicine for treating inflammatory diseases. R. principis crude polysaccharides with
anticomplementary activity demonstrated promising anti-inflammatory effects on acute lung injury induced by lipopolysaccharide. R. principis crude polysaccharides significantly
decreased the levels of TNF-α and interleukin-6 in both serum and blood and bronchoalveolar lavage fluid in lipopolysaccharide-induced acute lung injury mice by intragastric
administration (100 mg/kg). A heteropolysaccharide,...
Source: Planta Medica - May 11, 2023 Category: Drugs & Pharmacology Authors: Zhao, Zhi-Zhi Zhang, Jie Hong, Zhou Bao, Wei-Lian Zhou, Li-Shuang Liu, Yang Chen, Dao-Feng Lu, Yan Tags: Original Papers Source Type: research
Antiviral Effect of Natural and Semisynthetic Diterpenoids against Adenovirus Infection in vitro
In conclusion, diterpenes 2, 5 and 9 not only exert antiviral activity against adenovirus but also are able to restrain pro-inflammatory cytokines induced by the
virus. [...] Georg Thieme Verlag KG Rüdigerstraße 14, 70469 Stuttgart, GermanyArticle in Thieme eJournals: Table of contents | Abstract | Full text (Source: Planta Medica)
Source: Planta Medica - May 9, 2023 Category: Drugs & Pharmacology Authors: Bidart, Juan Esteban Pertino, Mariano Walter Schmeda-Hirschmann, Guillermo Alch é, Laura Edith Petrera, Erina Tags: Original Papers Source Type: research
LC-MS Analysis of Ginsenosides in Different Parts of Panax quinquefolius and Their Potential for Coronary Disease Improvement
Planta Med DOI: 10.1055/a-2058-1199Seven main ginsenosides, including ginsenoside Re, ginsenoside Rb1, pseudoginsenoside F11, ginsenoside Rb2, ginsenoside Rb3, ginsenoside Rd, and
ginsenoside F2, were identified by LC-QTOF MS/MS from root, leaf and flower extracts of Panax quinquefolius. These extracts promoted intersegmental vessel growth in a
zebrafish model, indicating their potential cardiovascular health benefits. Network pharmacology analysis was then conducted to reveal the potential mechanisms of ginsenoside activity in the
treatment of coronary artery disease. GO and KEGG enrichment analyses elucida...
Source: Planta Medica - April 17, 2023 Category: Drugs & Pharmacology Authors: Zhang, Xuanming Kong, Can Wang, Xixin Hou, Hairong Yu, Hongxia Wang, Lizhen Li, Peihai Li, Xiaobin Zhang, Yun Han, Liwen Liu, Kechun Tags: Original Papers Source Type: research
Curative Effect and Mechanisms of Radix Arnebiae Oil on Burn Wound Healing in Rats
Planta Med DOI: 10.1055/a-1997-5566Radix arnebiae oil (RAO) is a clinically useful traditional Chinese medical formula with outstanding curative effects on burns. However, the mechanism of the effect of RAO on wound healing
remains unclear. The present study investigates the molecular mechanisms of the potential curative effect of RAO on wound healing. The concentrations of the main constituents, shikonin,
imperatorin, and ferulic acid in RAO detected by HPLC were 24.57, 3.15, and 0.13 mg/mL, respectively. A rat burn model was established, and macroscopic and histopathological studies were
performed. RAO s...
Source: Planta Medica - April 17, 2023 Category: Drugs & Pharmacology Authors: Gao, Ting Zhao, Yu Zhao, Yuna He, Yanping Huang, Qi Yang, Jianhong Zhang, Liming Chen, Jing Tags: Original Papers Source Type: research
Anti-arthritis Effects of Zingiberaceae Extracts on Models of Inflammatory Joint Disease
This study investigates the anti-inflammatory and chondroprotective effects of ZO, CL, and KP extracts on in vitro and
ex vivo inflammatory models. The combinatorial anti-arthritis effect of each extract is also evaluated in an in vivo model. ZO extract preserves cartilaginous proteoglycans in
proinflammatory cytokines-induced porcine cartilage explant in a fashion similar to that of CL and KP extracts and suppresses the expression of major inflammatory mediators in SW982 cells,
particularly the COX2 gene. CL extract downregulates some inflammatory mediators and genes-associated cartilage degradation. Only K...
Source: Planta Medica - March 20, 2023 Category: Drugs & Pharmacology Authors: Kongdang, Patiwat Ongchai, Siriwan Chiranthanut, Natthakarn Pruksakorn, Dumnoensun Tags: Original Papers Source Type: research
Ouratea spectabilis and its Biflavanone Ouratein D Exert Potent Anti-inflammatory Activity in MSU Crystal-induced Gout in Mice
Planta Med DOI: 10.1055/a-2009-9809Gouty arthritis (GA) is an inflammatory arthritis triggered by the deposition of monosodium urate monohydrate (MSU) crystals, causing pain, inflammation, and joint damage. Several drugs are currently employed to manage acute flares of GA, but they either have limited effectiveness or induce severe adverse reactions. Ouratea spectabilis is traditionally used in Brazil to treat gastric ulcers and rheumatism. The ethanolic extract of O. spectabilis stems (OSpC) and four biflavanones (ouratein A – D) isolated thereof were evaluated in a murine model of GA induced by the injection of MSU...
Source: Planta Medica - March 15, 2023 Category: Drugs & Pharmacology Authors: Rocha, Marina P. Oliveira, Diego P. de Oliveira, Vivian L. S. Zaidan, Isabella Grossi, La ís C. Campana, Priscilla R. V. Amaral, Fl ávio A. Sousa, Lirl ândia P. Teixeira, Mauro M. Braga, Fern ão C. Tags: Original Papers Source Type: research
Acertannin Prevented Dextran Sulfate Sodium-induced Colitis by Inhibiting the Colonic Expression of IL-23 and TNF- α in C57BL/6J Mice
Planta Med DOI: 10.1055/a-2037-2995The present study investigates the effects of acertannin on colitis induced by dextran sulfate sodium (DSS) and changes in the colonic levels of the cytokines interleukin (IL)-1β, IL-6, IL-10, IL-23, tumor necrosis factor (TNF)-α, the chemokine monocyte chemoattractant protein (MCP)-1, and vascular endothelial growth factor (VEGF).We examine the following: inflammatory colitis was induced in mice by 2% DSS drinking water given ad libitum for 7 days. Red blood cell, platelets, and leukocyte counts and hematocrit (Ht), hemoglobin (Hb), and colonic cytokine and chemokine levels were measur...
Source: Planta Medica - March 15, 2023 Category: Drugs & Pharmacology Authors: Kimura, Yoshiyuki Taniguchi, Masahiko Okuda, Takuo Tags: Original Papers Source Type: research
Effects of Stevioside on the Expressions of GLUT 1, GLUT 3, and GLUT 4 Proteins in Diabetic Rat Placenta
Planta Med DOI: 10.1055/a-2003-9463The placenta provides maternal–fetal nutrient transport. The primary source of energy for fetus development is glucose and maternal–fetal glucose transport occurs through glucose transporters (GLUTs). Stevioside, a component of Stevia rebaudiana Bertoni, is used for medicinal and commercial purposes. We aim to determine the effects of stevioside on GLUT 1, GLUT 3, and GLUT 4 proteins expressions in diabetic rat placentas. The rats are divided into four groups. A single dose of streptozotocin (STZ) is administered to form the diabetic groups. Pregnant rats receive stevioside to form th...
Source: Planta Medica - March 13, 2023 Category: Drugs & Pharmacology Authors: Katirci, Ertan Kendirci-Katirci, Remziye Korgun, Emin Turkay Tags: Original Papers Source Type: research
Development of an Analytical Workflow to Support the Establishment of Monographs in African Pharmacopoeias – Combretum mucronatum Leaves as Example
Planta Med DOI: 10.1055/a-2002-2260Herbal medicines are invaluable in African medicine, but quality and safety are not documented in many cases. Besides controlled farming, validated quality control methods are needed to ensure identity, purity, and content. Analytical specifications within modern monographs are needed for consistent batch quality. Combretum mucronatum leaves are widely used in West Africa, but state-of-the-art quality control methods and specifications are non-existent. The aim of the following study was the development of ICH-validated chromatographic protocols for identity, purity, content assay, and an...
Source: Planta Medica - March 10, 2023 Category: Drugs & Pharmacology Authors: Orman, Emmanuel Bekoe, Samuel Oppong Asare-Nkansah, Samuel Kralisch, Ina Jato, Jonathan Spiegler, Verena Agyare, Christian Bekoe, Emelia Oppong Hensel, Andreas Tags: Original Papers Source Type: research
Multiple Component Pharmacokinetics after Oral Administration of Gnaphalium affine Extract in Rats
This study is aimed at investigating the individual and holistic pharmacokinetics of 10 bioactive components (including caffeic acid, caffeoylquinic acids, and flavonoids) in rats after single and multiple administrations of GAD. GAD is orally dosed to normal male rats at doses of 225, 450, or 900 mg/kg/day for 10 consecutive days and also orally administrated to uric acid nephropathy (UAN) rats at a dose of 900 mg/kg/day for 28 consecutive days. Integrated PKs of multiple components are calculated by area under the curve (AUC)-based weighting approach. All the components show a double-peak phenomenon in terms of their...
Source: Planta Medica - March 10, 2023 Category: Drugs & Pharmacology Authors: Han, Shiyi Liu, Xizi Chen, Ye Chen, Junping Han, Qinghua Fan, Siyang Tags: Original Papers Source Type: research
Oxidation Products from the Neolignan Licarin A by Biomimetic Reactions and Assessment of in vivo Acute Toxicity
Planta Med DOI: 10.1055/a-2009-0732Licarin A, a dihydrobenzofuranic neolignan presents in several medicinal plants and seeds of nutmeg, exhibits strong activity against protozoans responsible for Chagas disease and leishmaniasis. From biomimetic reactions by metalloporphyrin and Jacobsen catalysts, seven products were determined: four isomeric products yielded by epoxidation from licarin A, besides a new product yielded by a vicinal diol, a benzylic aldehyde, and an unsaturated aldehyde in the structure of the licarin A. The incubation with rat and human liver microsomes partially reproduced the biomimetic reactions by the...
Source: Planta Medica - March 8, 2023 Category: Drugs & Pharmacology Authors: Souza, Juliana Neves de Paula e da Silva, Rodrigo Moreira Fortes, Simone Silveira de Oliveira, Anderson Rodrigo Moraes Ferreira, Leandro S. Vessecchi, Ricardo Lopes, Norberto Peporine Silva, Denise Brentan Tags: Original Papers Source Type: research
