Development of a method to preliminarily embed tissue samples using low melting temperature fish gelatin before sectioning: A technical note
Embedding of tissue samples that maintains a desired orientation is critical for preparing sections suitable for diagnosis and study objectives. Methods to prepare tissue sections include: (i) paraffin embedding or snap‐freezing followed by microtome or cryostat sectioning; and (ii) agarose embedding followed by cutting on a vibrating microslicer. Although these methods are useful for routine laboratory work, preparation of small and fragile tissues such as mouse organs, small human biopsy samples, and cultured floating spheres is difficult and requires special skills. In particular, tissue specimen orientation can be lo...
Source: Pathology International - February 1, 2018 Category: Pathology Authors: Kaori Ushida, Naoya Asai, Kozo Uchiyama, Atsushi Enomoto, Masahide Takahashi Tags: Short Communication Source Type: research
Mesenchymal –epithelial transition of pancreatic cancer cells at perineural invasion sites is induced by Schwann cells
In conclusion, MET‐like change is induced by Schwann cells, suggesting that Schwann cells contribute to PDAC colonization in pancreatic nerves through activating the MET machinery inside tumor cells in the pancreatic tumor microenvironment. (Source: Pathology International)
Source: Pathology International - February 1, 2018 Category: Pathology Authors: Yoko Fujii ‐Nishimura, Ken Yamazaki, Yohei Masugi, Junya Douguchi, Yutaka Kurebayashi, Naoto Kubota, Hidenori Ojima, Minoru Kitago, Masahiro Shinoda, Akinori Hashiguchi, Michiie Sakamoto Tags: Original Article Source Type: research
A case of microscopic, multiple sclerosing pneumocytoma
Sclerosing pneumocytoma is a rare tumor of the lung, commonly affecting middle‐aged women, and is mostly isolated. Although this tumor is thought to be derived from primitive respiratory epithelial cells, the characteristics of the precursor cells are still unknown. A 19‐year‐old woman presented with multiple nodules in the right lung. Partial resection of the right middle lobe was performed, and seven sclerosing pneumocytomas, including four that were microscopic, were detected. The latter showed a simple papillary pattern, and three of them consisted of only round cell‐like cells (single population). Interestingl...
Source: Pathology International - February 1, 2018 Category: Pathology Authors: Hitomi Kawai, Norio Takayashiki, Haruo Otani, Shingo Sakashita, Masayuki Noguchi Tags: Case Report Source Type: research
Primary enteric ‐type tubulovillous adenocarcinoma arising in the renal pelvis
(Source: Pathology International)
Source: Pathology International - February 1, 2018 Category: Pathology Authors: Akihiro Shioya, Katsuhiko Saito, Nozomu Kurose, Yasukazu Takase, Koichi Kodama, Hiroshi Minato, Takayuki Nojima, Sohsuke Yamada Tags: Letter to the Editor Source Type: research
A rare case of uterine tumor resembling ovarian sex ‐cord tumor with necrosis and increased mitotic activity
(Source: Pathology International)
Source: Pathology International - February 1, 2018 Category: Pathology Authors: Nomoto Kazuhiro Tags: Letter To The Editor Source Type: research
Editorial Comment on Validation of the digital PCR system for use in tyrosine kinase inhibitor ‐resistant EGFR‐mutant non‐small‐cell lung cancer
(Source: Pathology International)
Source: Pathology International - February 1, 2018 Category: Pathology Authors: Haruhiko Sugimura, Tomoaki Kahyo Tags: Editorial Comment Source Type: research
Basigin expression as a prognostic indicator in stage I pulmonary adenocarcinoma
This study aimed to clarify the relationships between BSG expression and clinicopathological parameters and determine the prognostic significance of BSG expression in pulmonary adenocarcinoma (AC) patients. To evaluate the significance of BSG expression in lung cancer, we immunohistochemically analyzed 113 surgically resected pulmonary adenocarcinomas, and the associations between BSG expression and various clinicopathological parameters were evaluated. Kaplan‐Meier survival analysis and Cox proportional hazards models were used to investigate the effects of BSG expression on survival. Clinicopathologically, BSG expressi...
Source: Pathology International - February 1, 2018 Category: Pathology Authors: Toshihide Matsumoto, Ryo Nagashio, Shinichiro Ryuge, Satoshi Igawa, Makoto Kobayashi, Eriko Fukuda, Naoki Goshima, Masaaki Ichinoe, Shi ‐Xu Jiang, Yukitoshi Satoh, Noriyuki Masuda, Yoshiki Murakumo, Makoto Saegusa, Yuichi Sato Tags: Original Article Source Type: research
Stromal tiny black dots, like “sugar‐coated”, of von Kossa stain is a diagnostic clue to hyalinizing trabecular tumor of the thyroid gland
Pathology International,Volume 68, Issue 3, Page 176-182, March 2018. (Source: Pathology International)
Source: Pathology International - January 24, 2018 Category: Pathology Source Type: research
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Pathology International,Volume 68, Issue 3, Page 176-182, March 2018. (Source: Pathology International)
Source: Pathology International - January 24, 2018 Category: Pathology Source Type: research
Fulminant adenovirus hepatitis with adenovirus ‐associated esophagitis complicating malignant lymphoma
Pathology International, EarlyView. (Source: Pathology International)
Source: Pathology International - January 19, 2018 Category: Pathology Source Type: research
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Pathology International, Ahead of Print. (Source: Pathology International)
Source: Pathology International - January 19, 2018 Category: Pathology Source Type: research
Validation of the digital PCR system in tyrosine kinase inhibitor ‐resistant EGFR mutant non‐small‐cell lung cancer
Pathology International,Volume 68, Issue 3, Page 167-173, March 2018. (Source: Pathology International)
Source: Pathology International - January 17, 2018 Category: Pathology Source Type: research
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Pathology International,Volume 68, Issue 3, Page 167-173, March 2018. (Source: Pathology International)
Source: Pathology International - January 17, 2018 Category: Pathology Source Type: research
Validation of the digital PCR system in tyrosine kinase inhibitor ‐resistant EGFR mutant non‐small‐cell lung cancer
The aim of this study was to compare the accuracy of the QuantStudio 3D Digital polymerase chain reaction (dPCR) system and a PCR‐based next generation sequencing (NGS) system for detecting a secondary mutation in the epidermal growth factor receptor (EGFR) gene T790M in non‐small cell lung cancer (NSCLC) patients previously diagnosed with EGFR‐activating mutations. Twenty‐five patients with NSCLC previously treated with EGFR‐TKIs were examined. The patients were treated daily with either erlotinib or gefitinib. New biopsies, followed by DNA sequencing on an Ion Torrent systems using the Ion Torrent AmpliSeq Canc...
Source: Pathology International - January 17, 2018 Category: Pathology Authors: Katsuhiro Masago, Shiro Fujita, Akito Hata, Chiyuki Okuda, Yuko Yoshizumi, Reiko Kaji, Nobuyuki Katakami, Yukio Hirata, Yasushi Yatabe Tags: Original Article Source Type: research
Transparency ‐enhancing technology allows three‐dimensional assessment of gastrointestinal mucosa: A porcine model
Although high‐resolution three‐dimensional imaging of endoscopically resected gastrointestinal specimens can help elucidating morphological features of gastrointestinal mucosa or tumor, there are no established methods to achieve this without breaking specimens apart. We evaluated the utility of transparency‐enhancing technology for three‐dimensional assessment of gastrointestinal mucosa in porcine models. Esophagus, stomach, and colon mucosa samples obtained from a sacrificed swine were formalin‐fixed and paraffin‐embedded, and subsequently deparaffinized for analysis. The samples were fluorescently stained, o...
Source: Pathology International - January 17, 2018 Category: Pathology Authors: Hiroya Mizutani, Satoshi Ono, Tetsuo Ushiku, Yotaro Kudo, Masako Ikemura, Natsuko Kageyama, Nobutake Yamamichi, Mitsuhiro Fujishiro, Takao Someya, Masashi Fukayama, Kazuhiko Koike, Hiroshi Onodera Tags: Original Article Source Type: research
