Evolutionary trajectories of beta-lactamase NDM and DLST cluster in < em > Pseudomonas aeruginosa < /em > : finding the putative ancestor
Pathog Glob Health. 2023 Jul 18:1-12. doi: 10.1080/20477724.2023.2236416. Online ahead of print.ABSTRACTPseudomonas aeruginosa has different antibiotic resistance pathways, such as broad-spectrum lactamases and metallo-β-lactamases (MBL), penicillin-binding protein (PBP) alteration, and active efflux pumps. Polymerase chain reaction (PCR) and sequencing methods were applied for double-locus sequence typing (DLST) and New Delhi metallo-β-lactamase (NDM) typing. We deduced the evolutionary pathways for DLST and NDM genes of P. aeruginosa using phylogenetic network. Among the analyzed isolates, 62.50% of the P. aeruginosa i...
Source: Pathogens and Global Health - July 19, 2023 Category: International Medicine & Public Health Authors: Parisa Sadeghi Karim Mahnam Azhar Salari-Jazi Ashok Aspatwar Jamshid Faghri Source Type: research
Malaria diagnosis using a combined system of a simple and fast extraction method with a lyophilised Dual-LAMP assay in a non-endemic setting
This study aims to solve some limitations of LAMP method with the optimization and validation of six LAMP assays, genus and species-specific, using an easy and fast extraction method, the incorporation of a reaction control assay, two ways (Dual) of result reading and reagent lyophilization. The Dual-LAMP assays were validated against the Nested-Multiplex Malaria PCR. A conventional column and saline extraction methods, and the use of lyophilized reaction tubes were also assessed. A new reaction control Dual-LAMP-RC assay was designed. Dual-LAMP-Pspp assay showed no cross-reactivity with other parasites, repeatability and ...
Source: Pathogens and Global Health - July 7, 2023 Category: International Medicine & Public Health Authors: Alexandra Mart ín Ramírez Lourdes Bar ón Argos Marta Lanza Su árez Claudia Carmona Rubio Ana P érez-Ayala Shamilah R Hisam Jos é M Rubio Source Type: research
