Impact of Region-of-Interest Size on Immune Profiling Using Multiplex Immunofluorescence Tyramide Signal Amplification for Paraffin-Embedded Tumor Tissues
Conclusion: We found that at minimum 1.65 mm2 area is enough to represent the entire tumor areas in most of our NSCLC samples using mIF.Pathobiology (Source: Pathobiology)
Source: Pathobiology - May 24, 2022 Category: Pathology Source Type: research

Low-Dose Alcohol-Induced Inhibition of Mouse Orthotopically Transplanted Tumors Is Associated with T-Cell Response
Conclusion: Ingesting a threshold amount of alcohol results in the infiltration of T lymphocytes, which may enhance immune responsiveness in mouse colorectal cancer models.Pathobiology (Source: Pathobiology)
Source: Pathobiology - May 16, 2022 Category: Pathology Source Type: research

Aurora Kinase A Is an Independent Predictor of Invasive Recurrence in Breast Ductal Carcinoma in situ
Conclusion: High AURKA expression is associated with poor prognosis in DCIS and might be a potential marker to predict DCIS progression to invasive disease.Pathobiology (Source: Pathobiology)
Source: Pathobiology - May 9, 2022 Category: Pathology Source Type: research

Protocadherin B9 Is Associated with Tumorigenesis and Cancer Progression in Colorectal Cancer
Conclusion: These results suggest that protocadherin B9 might be associated with colorectal tumorigenesis and cancer progression in CRC.Pathobiology (Source: Pathobiology)
Source: Pathobiology - May 9, 2022 Category: Pathology Source Type: research

A Case of Acute Plastic Deformation of the Forearm in a Medieval Hispano-Mudejar Skeleton (13 –14th Centuries AD)
Conclusions: Acute plastic deformation is a rare traumatic injury, not described until the last century and only rarely described in palaeopathological contexts. We contribute a new case, the first being sufficiently documented, contributing to the knowledge and diagnosis of this type of trauma in the ancient bone, while deepening the knowledge of the living conditions of the medieval Mudejar population of Uceda.Pathobiology (Source: Pathobiology)
Source: Pathobiology - May 3, 2022 Category: Pathology Source Type: research

Unusual Patterns of HER2 Expression in Breast Cancer: Insights and Perspectives
The biomarker human epidermal growth factor receptor-2 (HER2) has represented the best example of successful targeted therapy in breast cancer patients. Based on the concept of “oncogene addiction,” we have learnt how to identify patients likely benefitting from anti-HER2 agents. SinceHER2 gene amplification leads to marked overexpression of the HER2 receptors on the cell membrane, immunohistochemistry with clinically validated antibodies and scoring system based on intensity and completeness of the membranous expression constitute the screening method to separate negative (score 0/1+) and positive (score 3+) carcinoma...
Source: Pathobiology - May 2, 2022 Category: Pathology Source Type: research

Molecularly Confirmed Female Donor-Transmitted Lobular Breast Cancer to Male following Renal Transplantation
Conclusion: Due to discordance between the donor and recipient sex, fluorescent in situ hybridization as a molecular technique correctly identified the origin of an individual ’s cancer in the post-transplant setting. The metastatic breast cancer behaved more indolently than usually seen. Expanded criteria donors (ECD) are those who cannot donate under standard criteria for organ transplantation; expanded criteria widen the potential organ donor pool at the expense of i ncreased risk for post-transplant complications (e.g., graft failure, the transmission of malignancy). The case provides a potential area of future resea...
Source: Pathobiology - May 2, 2022 Category: Pathology Source Type: research

Trophoblast Cell Surface Antigen 2 Expression in Human Tumors: A Tissue Microarray Study on 18,563 Tumors
Conclusion: TROP2 is expressed in many epithelial neoplasms. TROP2 deregulation can be associated with cancer progression in a tumor-type dependent manner. Since anti-TROP2 cancer drugs have demonstrated efficiency, they may be applicable to a broad range of tumor entities in the future.Pathobiology (Source: Pathobiology)
Source: Pathobiology - April 27, 2022 Category: Pathology Source Type: research

Quality Issues in Diagnostic Immunohistochemistry in Breast Pathology
Immunohistochemistry techniques have been incorporated into surgical pathology for nearly a half-century and have since become intimately intertwined with its practice. In the realm of breast pathology, immunohistochemistry serves several purposes, including providing crucial prognostic and predictive data. Among its other applications, assessment of stromal invasion and establishment of mammary origin are crucial from a diagnostic standpoint. In these regards, sole reliance on immunohistochemistry may lead to misdiagnosis. In this review, we highlight pitfalls of immunohistochemistry commonly encountered in the practice o...
Source: Pathobiology - April 20, 2022 Category: Pathology Source Type: research

Protocadherin B9 Is Associated with Human Esophageal Squamous Cell Carcinoma Progression
Conclusion: These results suggest that PCDHB9 plays a tumor-promoting role and is a potential biomarker and therapeutic target in ESCC.Pathobiology (Source: Pathobiology)
Source: Pathobiology - April 7, 2022 Category: Pathology Source Type: research

Immunohistochemistry Applied to Breast Cytological Material
Fine-needle aspiration biopsies (FNABs) of the breast are minimally invasive procedures enabling the diagnosis of suspicious breast lesions. Unfortunately, they are often perceived as inferior to core-needle biopsies, namely because they are supposedly unable to differentiate between high-grade ductal carcinoma in situ and invasive carcinoma or provide material for ancillary testing. Several studies have shown, however, that FNAB samples, when handled properly, are indeed capable of providing sufficient and adequate material for ancillary testing, namely immunocytochemistry (ICC). We reviewed the published literature regar...
Source: Pathobiology - April 1, 2022 Category: Pathology Source Type: research

Clinicopathologic and Molecular Characteristics of and Diagnostic Dilemmas in Invasive Breast Carcinoma with Choriocarcinomatous Pattern apropos a New Case: A Literature Review with New Findings
Conclusion: The present IBC-CP case is triple-negative breast cancer withTP53 mutation. ThePTEN gene may be associated with choriocarcinomatous differentiation. Obtaining a medical history is mandatory to exclude metastatic lesions.Pathobiology (Source: Pathobiology)
Source: Pathobiology - March 30, 2022 Category: Pathology Source Type: research

The Use of Pan-Tropomyosin Receptor Kinase Immunohistochemistry as a Screening Tool for the Detection of Neurotrophic Tropomyosin-Related Kinase Fusions: Real-World Data from a National Multicentric Retrospective Study
Conclusion: Pan-TRK IHC can be used to screen forNTRK fusions, especially in commonly diagnosed solid tumors with lowNTRK fusion prevalence. In case of pan-TRK immunoreactivity, regardless of its intensity and tumor cell percentage, subsequent molecular tests should be performed to formally confirm the presence or absence ofNTRK fusions.Pathobiology (Source: Pathobiology)
Source: Pathobiology - March 29, 2022 Category: Pathology Source Type: research

Implications of COVID-19 on Thrombotic Profile of Severely Affected Patients
Conclusion: COVID-19 infection severely impacts the blood coagulation cascade, which might lead to the manifestation of severe symptoms and increased mortality in patients.Pathobiology (Source: Pathobiology)
Source: Pathobiology - March 28, 2022 Category: Pathology Source Type: research

Long Non-Coding RNA HCG11 Inhibits Glioma Cells Proliferation and Migration through Decoying miR-590-3p and Up-Regulating CADM2
Conclusions: HCG11 suppresses glioma cells proliferation and migration through regulating the miR-590-3p/CADM2 molecular axis.Pathobiology (Source: Pathobiology)
Source: Pathobiology - March 11, 2022 Category: Pathology Source Type: research