CD34-Positive Blast Count and p53 Expression in Bone Marrow Biopsies of Patients with Low-Risk Myelodysplastic Syndromes: Potential Predictive Tools of Response to Erythropoietin Stimulating Agents
Conclusions: Our study reinforces the role of BM biopsy as diagnostic tool in MDSs, being also able to supply information related to response to ESAs and to its loss over time.Pathobiology (Source: Pathobiology)
Source: Pathobiology - February 15, 2021 Category: Pathology Source Type: research

Immune System Efficiency in Cancer and the Microbiota Influence
The immune system plays a critical role in preventing cancer development and progression. However, the complex network of cells and soluble factor that form the tumor microenvironment (TME) can dictate the differentiation of tumor-infiltrating leukocytes and shift the antitumor immune response into promoting tumor growth. With the advent of cancer immunotherapy, there has been a reinvigorated interest in defining how the TME shapes the antitumor immune response. This interest brought to light the microbiome as a novel player in shaping cancer immunosurveillance. Indeed, accumulating evidence now suggests that the microbiom...
Source: Pathobiology - February 15, 2021 Category: Pathology Source Type: research

Gastric Cancer Microbiome
Identifying a microbiome pattern in gastric cancer (GC) is hugely debatable due to the variation resulting from the diversity of the studied populations, clinical scenarios, and metagenomic approach.H. pylori remains the main microorganism impacting gastric carcinogenesis and seems necessary for the initial steps of the process. Nevertheless, an additional non-H. pylori microbiome pattern is also described, mainly at the final steps of the carcinogenesis. Unfortunately, most of the presented results are not reproducible, and there are no consensual candidates to share theH. pylori protagonists. Limitations to reach a consi...
Source: Pathobiology - February 15, 2021 Category: Pathology Source Type: research

Pleuropulmonary Blastoma with Hotspot Mutations in RNase IIIb Domain of DICER 1: Clinicopathologic Study of 10 Cases in a Single-Institute Experience
Conclusions: Late detection of solid PPB is associated with poor prognosis. Considering the rarity of PPB disease and the importance ofDICER1 hotspot mutation in pathogenesis,DICER1 hotspot mutation testing and identification in the early cystic stage can improve patient outcomes.Pathobiology (Source: Pathobiology)
Source: Pathobiology - February 10, 2021 Category: Pathology Source Type: research

Microbiota in Cancer Development
Pathobiology (Source: Pathobiology)
Source: Pathobiology - February 10, 2021 Category: Pathology Source Type: research

The Added Value of SOX10 Immunohistochemistry to Other Breast Markers in Identifying Cytokeratin 5-Positive Triple Negative Breast Cancers as of Mammary Origin
Discussion: SOX10 proved to be the most commonly positive breast marker in our CK5 expressing TNBCs, but the other markers also had some additive value to SOX10.Pathobiology (Source: Pathobiology)
Source: Pathobiology - February 10, 2021 Category: Pathology Source Type: research

Hyperglycemia Induces Generation of Reactive Oxygen Species and Accelerates Apoptotic Cell Death in Salivary Gland Cells
Conclusion: These findings suggest a novel mechanism whereby hyperglycemic-induced ROS production promotes salivary gland injury, resulting in hyposalivation.Pathobiology (Source: Pathobiology)
Source: Pathobiology - February 8, 2021 Category: Pathology Source Type: research

Subject Index
Pathobiology 2021;88:109 (Source: Pathobiology)
Source: Pathobiology - January 20, 2021 Category: Pathology Source Type: research

Author Index
Pathobiology 2021;88:108 (Source: Pathobiology)
Source: Pathobiology - January 20, 2021 Category: Pathology Source Type: research

Prelims
Pathobiology 2021;88:7 –10 (Source: Pathobiology)
Source: Pathobiology - January 20, 2021 Category: Pathology Source Type: research

CXC Chemokine Receptor Type 5 Gene Polymorphisms in a Cohort of Egyptian Patients with Diffuse Large B-Cell Lymphoma
Conclusions:CXCR5 gene polymorphisms could be considered a potential risk factor for the development of DLBCL.Pathobiology (Source: Pathobiology)
Source: Pathobiology - December 30, 2020 Category: Pathology Source Type: research

Microbiome: A Supportive or a Leading Actor in Lung Cancer?
Lung cancer is still the leading cause of cancer death worldwide. Despite the major diagnostic and therapeutic innovations, the effect on mortality has been modest and the overall survival is still poor. Better understanding of the pathology of these tumors is necessary in order to develop personalized therapeutic strategies in lung cancer patients. Human microbiome has been associated with normal physiology and function, and increasing evidence points towards a key role of the microbiome in promoting the progression of lung disease. Studies have shown that although poorly understood, lung has a distinctive microbiome that...
Source: Pathobiology - December 22, 2020 Category: Pathology Source Type: research

Investigations of Pathologists as a Key to Understanding Coronavirus Disease 2019
Pathobiology (Source: Pathobiology)
Source: Pathobiology - December 18, 2020 Category: Pathology Source Type: research

Publisher's Note
Pathobiology (Source: Pathobiology)
Source: Pathobiology - December 18, 2020 Category: Pathology Source Type: research

Goodbye and Thank You
Pathobiology (Source: Pathobiology)
Source: Pathobiology - December 18, 2020 Category: Pathology Source Type: research

New Editor-in-Chief of < b > < i > Pathobiology < /i > < /b > , the Journal of Translational Pathology
Pathobiology (Source: Pathobiology)
Source: Pathobiology - December 17, 2020 Category: Pathology Source Type: research

Tumor-Infiltrating T Cells Concurrently Overexpress CD200R with Immune Checkpoints PD-1, CTLA-4, and TIM-3 in Non-Small-Cell Lung Cancer
Conclusions: Our results showed that those tumors were dominated by T cells expressing CD200R together with other checkpoints, which suggests a phenotypic change after T cell infiltration into the tumor, such as T cell exhaustion.Pathobiology (Source: Pathobiology)
Source: Pathobiology - December 15, 2020 Category: Pathology Source Type: research

Contents, Vol. 87, 2020
Pathobiology 2020;87:I –IV (Source: Pathobiology)
Source: Pathobiology - December 15, 2020 Category: Pathology Source Type: research

Acknowledgement to Reviewers
Pathobiology 2020;87:375 –376 (Source: Pathobiology)
Source: Pathobiology - December 15, 2020 Category: Pathology Source Type: research

Biosafety Requirements for Autopsies of Patients with COVID-19: Example of a BSL-3 Autopsy Facility Designed for Highly Pathogenic Agents
Information obtained from autopsies of patients infected with high-risk pathogens is an important pillar in managing a proper response to pandemics, particular in the early phase. This is due to the fact that autopsy allows efficient evaluation of comorbidities for risk assessment, as well as identification of key pathophysiological and molecular mechanisms in organs driving the severity of disease which might be important targets for therapeutic interventions. In the case of patients who have died of infection with unknown pathogens, isolation and culture of pathogens from the affected organs is another important opportun...
Source: Pathobiology - December 9, 2020 Category: Pathology Source Type: research

The Upper Digestive Tract Microbiome and Oesophageal Squamous Cell Carcinoma: Epidemiology, Pathogenesis, and Clinical Implications in Africa
The study of the microbiome has significantly contributed to our understanding of complex diseases including cancer, with a profound influence of the microbiota on clinical prognosis and the efficacy of cancer treatments. Oesophageal cancer is positioned amongst the most aggressive malignant diseases, resulting from a complex interaction between anthropometric, genetic, immune response, and environmental factors. Oesophageal squamous cell carcinoma (OSCC) is the most common type of oesophageal cancer and is a serious burden in Eastern Africa, in the area known as the African oesophageal cancer corridor (AOCC). OSCC is ofte...
Source: Pathobiology - December 8, 2020 Category: Pathology Source Type: research

The Role of < b > < i > Fusobacterium nucleatum < /i > < /b > in Colorectal Carcinogenesis
Colorectal cancer (CRC) is one of the most frequent and deadly neoplasms worldwide. Genetic factors, lifestyle habits, and inflammation are important risk factors associated with CRC development. In recent years, growing evidence has supporting the significant role of the intestinal microbiome in CRC carcinogenesis. Disturbances in the healthy microbial balance, known as dysbiosis, are frequently observed in these patients. Pathogenic microorganisms that induce intestinal dysbiosis have become an important target to determine the role of bacterial infection in tumorigenesis. Interestingly, the presence of different bacteri...
Source: Pathobiology - December 8, 2020 Category: Pathology Source Type: research

miR-6838-5p Affects Cell Growth, Migration, and Invasion by Targeting GPRIN3 via the Wnt/ β-Catenin Signaling Pathway in Gastric Cancer
Gastric cancer (GC) is a highly prevalent digestive malignant tumor, ranking second in the tumor-related mortality globally. The microRNAs have been confirmed to be connected with GC progression. Accumulative evidence has suggested that miR-6838-5p exerts a suppressive effect on human cancers. Nonetheless, whether miR-6838-5p is involved in the regulation of GC remains to be investigated. During our research, miR-6838-5p was downregulated in GC cells. Upregulated miR-6838-5p repressed GC cell cycle progression, proliferation, migration, and invasion. Furthermore, miR-6838-5p overexpression repressed the nuclear import of &...
Source: Pathobiology - November 30, 2020 Category: Pathology Source Type: research

COVID-19 Autopsies: A Case Series from Poland
This study revealed thrombotic complications of COVID-19 and interstitial pneumonia with DAD presence as the main autopsy findings in patients with SARS-CoV-2 infection that was confirmed antemortem with molecular tests. Autopsy studies using tissue sections handled in accordance withSARS-CoV-2 biosafety guidelines are urgently needed, especially in the case of subjects who were below the age of 60.Pathobiology (Source: Pathobiology)
Source: Pathobiology - November 30, 2020 Category: Pathology Source Type: research

LncRNA MAFG-AS1 Promotes the Progression of Bladder Cancer by Targeting the miR-143-3p/COX-2 Axis
Conclusion: The MAFG-AS1/miR-143-3p/COX-2 axis contributes to BLCA progression.Pathobiology (Source: Pathobiology)
Source: Pathobiology - November 25, 2020 Category: Pathology Source Type: research

Regulatory Single Nucleotide Polymorphism Increases < b > < i > TERT < /i > < /b > Promoter Activity in Thyroid Carcinoma Cells
Conclusion: We conclude that the presence of rs2853669 within theTERT promoter could be as significant as the C228T mutation in thyroid carcinoma.Pathobiology (Source: Pathobiology)
Source: Pathobiology - November 23, 2020 Category: Pathology Source Type: research

Microbiome and Cervical Cancer
Persistent infection with some types of mucosal human papillomavirus (HPV) is the etiological factor for the development of cervical cancer and its precursor lesions. Besides, several cofactors are known to play a role in cervical disease onset and progression either by favoring or by preventing HPV infection and persistence. The microbiome of a healthy female genital tract is characterized by the presence of 1 or few varieties of lactobacilli. However, high-throughput studies addressing the bacterial diversity and abundance in the female genital tract have shown that several factors, including hormonal levels, hygiene hab...
Source: Pathobiology - November 23, 2020 Category: Pathology Source Type: research

Oral Microbiota and Cancer Development
Oral microbiota are among the most diverse in the human body. More than 700 species have been identified in the mouth, and new sequencing methods are allowing us to discover even more species. The anatomy of the oral cavity is different from that of other body sites. The oral cavity has mucosal surfaces (the tongue, the buccal mucosa, the gingiva, and the palate), hard tissues (the teeth), and exocrine gland tissue (major and minor salivary glands), all of which present unique features for microbiota composition. The connection between oral microbiota and diseases of the human body has been under intensive research in the ...
Source: Pathobiology - November 11, 2020 Category: Pathology Source Type: research

Retrospective Post-mortem SARS-CoV-2 RT-PCR of Autopsies with COVID-19-Suggestive Pathology Supports the Absence of Lethal Community Spread in Basel, Switzerland, before February 2020
Conclusion: Our data suggest the absence of early lethal community spread of COVID-19 in Basel before its initial reported emergence in Switzerland in March 2020.Pathobiology (Source: Pathobiology)
Source: Pathobiology - November 6, 2020 Category: Pathology Source Type: research

Gastric Cancer in < b > < i > BRCA1 < /i > < /b > Germline Mutation Carriers: Results of Endoscopic Screening and Molecular Analysis of Tumor Tissues
Conclusion: Taken together, these data justify further studies on the relationships between theBRCA1/2 and gastric cancer.Pathobiology (Source: Pathobiology)
Source: Pathobiology - November 6, 2020 Category: Pathology Source Type: research

A Covid-19 Patient with Complement-Mediated Coagulopathy and Severe Thrombosis
We report a patient with severe Covid-19-associated coagulopathy and type 2 diabetes mellitus who tested positive for antiphospholipid antibodies (aPL). Analysis of skin specimens suggested direct SARS-CoV-2 viral-induced and complement-mediated vascular injury and thrombosis, consistent with prior reports. Serial aPL testing demonstrated high levels of anticardiolipin antibodies (aCL) that declined to insignificant levels over a period of 5 weeks. SARS-CoV-2 RNA was detected in nasopharyngeal swab specimens on serial assays performed over the same 5-week period, though it was not detected thereafter. We hypothesize that S...
Source: Pathobiology - November 2, 2020 Category: Pathology Source Type: research

The Liver in COVID-19-Related Death: Protagonist or Innocent Bystander?
Conclusion: The main histological changes can be explained by the hypoxic status as a result of severe hypoxemic pneumonia leading to death. Drug toxicity may also play a role in certain cases. Other histological changes may be explained by previous hepatic conditions or underlying hepatic diseases. We concluded that COVID-19 infection was not associated with a specific histopathological pattern of the liver.Pathobiology (Source: Pathobiology)
Source: Pathobiology - October 27, 2020 Category: Pathology Source Type: research

In situ Evidence of Collagen V and Interleukin-6/Interleukin-17 Activation in Vascular Remodeling of Experimental Pulmonary Hypertension
In conclusion, our findings indicate in situ evidence of Col V and IL-6/IL-17 activation in vascular remodeling and suggest that increase of Col V may yield potential therapeutic targets for treating pa tients with PAH.Pathobiology (Source: Pathobiology)
Source: Pathobiology - October 23, 2020 Category: Pathology Source Type: research

The Impact of COVID-19 Disease on Platelets and Coagulation
Coronavirus disease 2019 (COVID-19) causes a spectrum of disease; some patients develop a severe proinflammatory state which can be associated with a unique coagulopathy and procoagulant endothelial phenotype. Initially, COVID-19 infection produces a prominent elevation of fibrinogen and D-dimer/fibrin(ogen) degradation products. This is associated with systemic hypercoagulability and frequent venous thromboembolic events. The degree of D-dimer elevation positively correlates with mortality in COVID-19 patients. COVID-19 also leads to arterial thrombotic events (including strokes and ischemic limbs) as well as microvascula...
Source: Pathobiology - October 13, 2020 Category: Pathology Source Type: research

Application of “Tissueoid Cell Culture System” Using a Silicate Fiber Scaffold for Cancer Research
Conclusion: Because the morphology of cancer cells cultured in this 3D culture system is similar to that in living organisms, we called the system a “tissueoid cell culture system.” Coating with collagen IV enables the modification of cell-matrix interactions as well as recapitulation of the in vivo microenvironment.Pathobiology (Source: Pathobiology)
Source: Pathobiology - September 23, 2020 Category: Pathology Source Type: research

Placental Pathology Findings during and after SARS-CoV-2 Infection: Features of Villitis and Malperfusion
We present a series of five placentas of SARS coronavirus 2 (SARS-CoV-2)-positive women who had been diagnosed with mild symptoms of COVID-19 or had been asymptomatic before birth. We provide a detailed histopathologic description of morphological changes accompanied by an analysis of presence of SARS-CoV-2 in the placental tissue. All placentas were term deliveries (40th and 41st gestational weeks). One SARS-CoV-2-positive patient presented with cough and dyspnoea. This placenta showed prominent lymphohistiocytic villitis and intervillositis and signs of maternal and foetal malperfusion. Viral RNA was present in both plac...
Source: Pathobiology - September 18, 2020 Category: Pathology Source Type: research

Autopsy Findings in 32 Patients with COVID-19: A Single-Institution Experience
Conclusion: This series of autopsies from patients with COVID-19 confirms the observation that the majority of severely affected patients have significant pulmonary pathology. However, many patients also have widespread microscopic thromboses, as well as characteristic findings in the liver and lymph nodes.Pathobiology (Source: Pathobiology)
Source: Pathobiology - September 17, 2020 Category: Pathology Source Type: research

Long Noncoding RNA HOTAIR Promotes Epithelial-Mesenchymal Transition and Is a Suitable Target to Inhibit Peritoneal Dissemination in Human Scirrhous Gastric Cancers
Conclusion: An EMT-associated pathway (the HOTAIR-miR-217-ZEB1 axis) appears to inhibit peritoneal dissemination and could lead to a novel therapeutic strategy against scirrhous gastric cancer in humans.Pathobiology (Source: Pathobiology)
Source: Pathobiology - September 16, 2020 Category: Pathology Source Type: research

High Activation of the AKT Pathway in Human Multicystic Renal Dysplasia
This study demonstrates the essential and specific role of the PI3K-AKT-mTOR pathway in the formation of cysts in multicystic renal dysplasia.Pathobiology (Source: Pathobiology)
Source: Pathobiology - September 14, 2020 Category: Pathology Source Type: research

Radiation-Associated Primary Osteosarcoma of the Breast
Conclusion: Post-irradiation osteosarcoma should be considered in the differential diagnosis of breast lesions showing malignant osteoid. Extensive sampling and careful search for epithelial differentiation is required to guide management. Complete surgical excision is recommended.Pathobiology (Source: Pathobiology)
Source: Pathobiology - September 11, 2020 Category: Pathology Source Type: research

Calcium Influx Kinetics and the Characteristics of Potassium Channels in Peripheral T Lymphocytes in Systemic Sclerosis
Conclusion: Th1 and CD8 cells demonstrate specific activation dynamics and sensitivity to potassium channel inhibition in SSc, distinguishing this condition both from healthy controls and other autoimmune diseases.Pathobiology (Source: Pathobiology)
Source: Pathobiology - September 10, 2020 Category: Pathology Source Type: research

Editorial
Pathobiology (Source: Pathobiology)
Source: Pathobiology - August 31, 2020 Category: Pathology Source Type: research

Unusual Presentation of Mammary Calciphylaxis in a Patient on Long-Standing Renal Dialysis
Conclusion: The hallmark of calciphylaxis is the deposition of calcium within small- to medium-sized vessel walls. This leads to ischaemia and necrosis of tissue. In the breast, only a few cases were reported in which patients presented with a necrotic lesion resembling carcinoma. These are reviewed in this report.Pathobiology (Source: Pathobiology)
Source: Pathobiology - August 19, 2020 Category: Pathology Source Type: research

Hydrophobicity and the Physico-Chemical Basis of Immunotolerance
This study analyzes the primary electrostatic interaction between the membrane-bound B-cell receptor and antigen peptide determinants, and identifies peptide frequency and hydrophobicity as the main factors that govern and shape immunotolerance versus immunoreactivity.Pathobiology (Source: Pathobiology)
Source: Pathobiology - July 29, 2020 Category: Pathology Source Type: research

The Two Sides of Cytopathology during the COVID-19 Health Emergency: Screening versus Diagnosis
Pathobiology (Source: Pathobiology)
Source: Pathobiology - July 21, 2020 Category: Pathology Source Type: research

Molecular Complexity of Lymphovascular Invasion: The Role of Cell Migration in Breast Cancer as a Prototype
Lymphovascular invasion (LVI) is associated with poor outcome in breast cancer (BC); however, its underlying mechanisms remain ill-defined. LVI in BC develops through complex molecular pathways involving not only the interplay with the surrounding microenvironment along with endothelial cells lining the lymphovascular spaces but also changes in the malignant epithelial cells with the acquisition of more invasive and migration abilities. In this review, we focus on the key features that enable tumour cell detachment from the primary niche, their migration and interaction with the surrounding microenvironment as well as the ...
Source: Pathobiology - July 9, 2020 Category: Pathology Source Type: research

NudCD1 Promotes the Proliferation and Metastasis of Non-Small Cell Lung Cancer Cells through the Activation of IGF1R-ERK1/2
Conclusion: NudCD1 promotes the proliferation and metastasis of NSCLC cells via activation of IGF1R-ERK1/2, which indicates that NudCD1 may be a potential therapy target of NSCLC.Pathobiology (Source: Pathobiology)
Source: Pathobiology - July 7, 2020 Category: Pathology Source Type: research

The Impact of the COVID-19 Pandemic and the Associated Belgian Governmental Measures on Cancer Screening, Surgical Pathology and Cytopathology
Conclusions: Since many health care interventions are reflected in the workload of a pathology laboratory, this study enabled us to identify areas for “deconfinement” health care actions. Our findings indicate that various areas in medicine were affected, but the impact seemed largest for cancer screening. Health care professionals should assure that consultations related to cancer screening are postponed instead of cancelled.Pathobiology (Source: Pathobiology)
Source: Pathobiology - July 7, 2020 Category: Pathology Source Type: research

SPC18 Expression Is an Independent Prognostic Indicator of Patients with Esophageal Squamous Cell Carcinoma
Conclusion: Specific inhibitors of SPC18 may be promising anticancer drugs for patients with ESCC.Pathobiology (Source: Pathobiology)
Source: Pathobiology - June 19, 2020 Category: Pathology Source Type: research

Immunomorphological Features of the Placenta in Allogeneic Pregnancy as the Background for the Development of Obstetric Complications
Conclusion: Placentas after IVF with oocyte donation and surrogate motherhood programs are characterized by similar changes, associated with the development of chronic inflammation in the structures of the placenta and immunohistochemical signs of impaired immunological tolerance at the maternal-fetal interface. The data we obtained allow us to classify pregnancies under surrogate motherhood programs as a risk factor for the development of pregnancy complications with immune pathogenesis.Pathobiology (Source: Pathobiology)
Source: Pathobiology - May 20, 2020 Category: Pathology Source Type: research