Application of “Tissueoid Cell Culture System” Using a Silicate Fiber Scaffold for Cancer Research
Conclusion: Because the morphology of cancer cells cultured in this 3D culture system is similar to that in living organisms, we called the system a “tissueoid cell culture system.” Coating with collagen IV enables the modification of cell-matrix interactions as well as recapitulation of the in vivo microenvironment.Pathobiology (Source: Pathobiology)
Source: Pathobiology - September 23, 2020 Category: Pathology Source Type: research

Placental Pathology Findings during and after SARS-CoV-2 Infection: Features of Villitis and Malperfusion
We present a series of five placentas of SARS coronavirus 2 (SARS-CoV-2)-positive women who had been diagnosed with mild symptoms of COVID-19 or had been asymptomatic before birth. We provide a detailed histopathologic description of morphological changes accompanied by an analysis of presence of SARS-CoV-2 in the placental tissue. All placentas were term deliveries (40th and 41st gestational weeks). One SARS-CoV-2-positive patient presented with cough and dyspnoea. This placenta showed prominent lymphohistiocytic villitis and intervillositis and signs of maternal and foetal malperfusion. Viral RNA was present in both plac...
Source: Pathobiology - September 18, 2020 Category: Pathology Source Type: research

Autopsy Findings in 32 Patients with COVID-19: A Single-Institution Experience
Conclusion: This series of autopsies from patients with COVID-19 confirms the observation that the majority of severely affected patients have significant pulmonary pathology. However, many patients also have widespread microscopic thromboses, as well as characteristic findings in the liver and lymph nodes.Pathobiology (Source: Pathobiology)
Source: Pathobiology - September 17, 2020 Category: Pathology Source Type: research

Long Noncoding RNA HOTAIR Promotes Epithelial-Mesenchymal Transition and Is a Suitable Target to Inhibit Peritoneal Dissemination in Human Scirrhous Gastric Cancers
Conclusion: An EMT-associated pathway (the HOTAIR-miR-217-ZEB1 axis) appears to inhibit peritoneal dissemination and could lead to a novel therapeutic strategy against scirrhous gastric cancer in humans.Pathobiology (Source: Pathobiology)
Source: Pathobiology - September 16, 2020 Category: Pathology Source Type: research

High Activation of the AKT Pathway in Human Multicystic Renal Dysplasia
This study demonstrates the essential and specific role of the PI3K-AKT-mTOR pathway in the formation of cysts in multicystic renal dysplasia.Pathobiology (Source: Pathobiology)
Source: Pathobiology - September 14, 2020 Category: Pathology Source Type: research

Radiation-Associated Primary Osteosarcoma of the Breast
Conclusion: Post-irradiation osteosarcoma should be considered in the differential diagnosis of breast lesions showing malignant osteoid. Extensive sampling and careful search for epithelial differentiation is required to guide management. Complete surgical excision is recommended.Pathobiology (Source: Pathobiology)
Source: Pathobiology - September 11, 2020 Category: Pathology Source Type: research

Calcium Influx Kinetics and the Characteristics of Potassium Channels in Peripheral T Lymphocytes in Systemic Sclerosis
Conclusion: Th1 and CD8 cells demonstrate specific activation dynamics and sensitivity to potassium channel inhibition in SSc, distinguishing this condition both from healthy controls and other autoimmune diseases.Pathobiology (Source: Pathobiology)
Source: Pathobiology - September 10, 2020 Category: Pathology Source Type: research

Editorial
Pathobiology (Source: Pathobiology)
Source: Pathobiology - August 31, 2020 Category: Pathology Source Type: research

Unusual Presentation of Mammary Calciphylaxis in a Patient on Long-Standing Renal Dialysis
Conclusion: The hallmark of calciphylaxis is the deposition of calcium within small- to medium-sized vessel walls. This leads to ischaemia and necrosis of tissue. In the breast, only a few cases were reported in which patients presented with a necrotic lesion resembling carcinoma. These are reviewed in this report.Pathobiology (Source: Pathobiology)
Source: Pathobiology - August 19, 2020 Category: Pathology Source Type: research

Hydrophobicity and the Physico-Chemical Basis of Immunotolerance
This study analyzes the primary electrostatic interaction between the membrane-bound B-cell receptor and antigen peptide determinants, and identifies peptide frequency and hydrophobicity as the main factors that govern and shape immunotolerance versus immunoreactivity.Pathobiology (Source: Pathobiology)
Source: Pathobiology - July 29, 2020 Category: Pathology Source Type: research

The Two Sides of Cytopathology during the COVID-19 Health Emergency: Screening versus Diagnosis
Pathobiology (Source: Pathobiology)
Source: Pathobiology - July 21, 2020 Category: Pathology Source Type: research

Molecular Complexity of Lymphovascular Invasion: The Role of Cell Migration in Breast Cancer as a Prototype
Lymphovascular invasion (LVI) is associated with poor outcome in breast cancer (BC); however, its underlying mechanisms remain ill-defined. LVI in BC develops through complex molecular pathways involving not only the interplay with the surrounding microenvironment along with endothelial cells lining the lymphovascular spaces but also changes in the malignant epithelial cells with the acquisition of more invasive and migration abilities. In this review, we focus on the key features that enable tumour cell detachment from the primary niche, their migration and interaction with the surrounding microenvironment as well as the ...
Source: Pathobiology - July 9, 2020 Category: Pathology Source Type: research

NudCD1 Promotes the Proliferation and Metastasis of Non-Small Cell Lung Cancer Cells through the Activation of IGF1R-ERK1/2
Conclusion: NudCD1 promotes the proliferation and metastasis of NSCLC cells via activation of IGF1R-ERK1/2, which indicates that NudCD1 may be a potential therapy target of NSCLC.Pathobiology (Source: Pathobiology)
Source: Pathobiology - July 7, 2020 Category: Pathology Source Type: research

The Impact of the COVID-19 Pandemic and the Associated Belgian Governmental Measures on Cancer Screening, Surgical Pathology and Cytopathology
Conclusions: Since many health care interventions are reflected in the workload of a pathology laboratory, this study enabled us to identify areas for “deconfinement” health care actions. Our findings indicate that various areas in medicine were affected, but the impact seemed largest for cancer screening. Health care professionals should assure that consultations related to cancer screening are postponed instead of cancelled.Pathobiology (Source: Pathobiology)
Source: Pathobiology - July 7, 2020 Category: Pathology Source Type: research

SPC18 Expression Is an Independent Prognostic Indicator of Patients with Esophageal Squamous Cell Carcinoma
Conclusion: Specific inhibitors of SPC18 may be promising anticancer drugs for patients with ESCC.Pathobiology (Source: Pathobiology)
Source: Pathobiology - June 19, 2020 Category: Pathology Source Type: research

Immunomorphological Features of the Placenta in Allogeneic Pregnancy as the Background for the Development of Obstetric Complications
Conclusion: Placentas after IVF with oocyte donation and surrogate motherhood programs are characterized by similar changes, associated with the development of chronic inflammation in the structures of the placenta and immunohistochemical signs of impaired immunological tolerance at the maternal-fetal interface. The data we obtained allow us to classify pregnancies under surrogate motherhood programs as a risk factor for the development of pregnancy complications with immune pathogenesis.Pathobiology (Source: Pathobiology)
Source: Pathobiology - May 20, 2020 Category: Pathology Source Type: research

Subject Index
Pathobiology 2020;87:160 (Source: Pathobiology)
Source: Pathobiology - May 19, 2020 Category: Pathology Source Type: research

Author Index
Pathobiology 2020;87:159 (Source: Pathobiology)
Source: Pathobiology - May 19, 2020 Category: Pathology Source Type: research

title page / table of contents
Pathobiology 2020;87:51 –54 (Source: Pathobiology)
Source: Pathobiology - May 19, 2020 Category: Pathology Source Type: research

Lack of Association between IFNL3 Polymorphism and Human Papillomavirus Infection and Their Progression in HIV-Infected Women Receiving Antiretroviral Treatment
Conclusions: Our results showed that theIFNL3 rs12979860 polymorphism is not a major determinant of the susceptibility to HPV infection and their progression to abnormal cervical lesions in women living with HIV.Pathobiology (Source: Pathobiology)
Source: Pathobiology - May 19, 2020 Category: Pathology Source Type: research

Mutational Profiling of Driver Tumor Suppressor and Oncogenic Genes in Brazilian Malignant Pleural Mesotheliomas
Conclusions: We concluded that Brazilian MPM harbor mutation in classic tumor suppressor and oncogenic genes, which might help in the guidance of personalized treatment of MPM.Pathobiology 2020;87:208 –215 (Source: Pathobiology)
Source: Pathobiology - May 5, 2020 Category: Pathology Source Type: research

Erythromycin Regulates Cigarette Smoke-Induced Proinflammatory Mediator Release Through Sirtuin 1-Nuclear Factor κB Axis in Macrophages and Mice Lungs
Conclusion: These findings suggest that EM increased SIRT1, leading to acetylation/expression of NF- κB, and thereby decreasing cigarette smoke-driven NF-κB-dependent proinflammatory cytokine.Pathobiology (Source: Pathobiology)
Source: Pathobiology - June 26, 2019 Category: Pathology Source Type: research

Comparison of Quantitative Analysis of Methylated Alleles Real-Time PCR and Methylation-Specific MLPA for Molecular Diagnosis of Beckwith-Wiedemann Syndrome
Conclusions: The small sample size reflects the rare nature of this epigenetic disorder, and the range of ages was quite wide, as was the degree of disease severity. Therefore, further validation with larger cohorts is warranted.Pathobiology (Source: Pathobiology)
Source: Pathobiology - June 25, 2019 Category: Pathology Source Type: research

Association of MTHFR C677T, MTHFR A1298C, and MTRR A66G Polymorphisms with Neural Tube Defects in Tunisian Parents
Conclusion: Additional studies are required to better understand the roles of parental gene polymorphisms related to folate-homocysteine metabolism in the pathogenesis of NTD.Pathobiology (Source: Pathobiology)
Source: Pathobiology - June 25, 2019 Category: Pathology Source Type: research

< b > < i > SEC11A < /i > < /b > Expression Is Associated with Basal-Like Bladder Cancer and Predicts Patient Survival
Conclusion: These results indicate that SPC18 plays an important role in the progression of BC. Specific inhibitors of SPC18 may be promising anticancer drugs for patients with basal-like BC.Pathobiology (Source: Pathobiology)
Source: Pathobiology - June 4, 2019 Category: Pathology Source Type: research

Concurrent Expression of CD47 and CD44 in Colorectal Cancer Promotes Malignancy
In this study, expression of CD47 was examined by immunostaining in colorectal cancer (CRC) and compared with the expression of CD44, which is a marker for cancer stem cells. In 95 cases of stage II –IV CRC, CD47 and CD44 showed overexpression in 82 and 80 cases, respectively. Both expression levels correlated with distant metastasis. Moreover, the expression of CD47 and CD44 in each case showed a significant correlation. In stage III cases, disease-free survival of cases showing high express ion of CD47 and CD44 was worse than that of the cases with low expression. Furthermore, 3 of the stage IV cases were administe...
Source: Pathobiology - May 27, 2019 Category: Pathology Source Type: research

Suppressed Expression but Not Activity of Collagenases MMP-1 and MMP-13 in Human Renal Carcinoma
Conclusions: The lower content and higher activity of the collagenases investigated in cancer tissue indicate that most of these enzymes are in active form in renal carcinoma. The lower collagen content in cancer tissue can be explained at least in part by increased collagenase activity.Pathobiology (Source: Pathobiology)
Source: Pathobiology - May 25, 2019 Category: Pathology Source Type: research

MicroRNA-147b Promotes Proliferation and Invasion of Human Colorectal Cancer by Targeting RAS Oncogene Family (RAP2B)
Conclusions: miR-147b not only plays important roles in the regulation of cell proliferation and tumor growth in CRC, which might be a potential prognostic marker or therapeutic target for CRC.Pathobiology (Source: Pathobiology)
Source: Pathobiology - May 23, 2019 Category: Pathology Source Type: research

Immunostaining of Increased Expression of Enhancer of Zeste Homolog 2 (EZH2) in Diffuse Midline Glioma H3K27M-Mutant Patients with Poor Survival
Conclusion: We suggest that the expression of EZH2 is not associated with the PRC2 pathway and increases in patients with H3K27M-mutant diffuse midline glioma and a poor prognosis. Further studies are necessary to understand the mechanism involved.Pathobiology (Source: Pathobiology)
Source: Pathobiology - May 17, 2019 Category: Pathology Source Type: research

Eradication of < b > < i > Helicobacter pylori < /i > < /b > Induces Immediate Regressive Changes in Early Gastric Adenocarcinomas
Conclusion:H. pylori eradication rapidly causes cancer regression and leads to tumor shrinkage, diminished atypism, and shortened proliferative zone, resulting in drastic morphological changes.Pathobiology (Source: Pathobiology)
Source: Pathobiology - March 15, 2019 Category: Pathology Source Type: research

No Evidence of Microsatellite Instability and Loss of Mismatch-Repair-Protein Expression in Squamous Cell Carcinoma of the Penis
Conclusion: MSI and defects in MMR protein expression are not regular features of penile SCC and might not act as biomarkers for PD-1/PD-L1 blockade therapy in penile carcinoma.Pathobiology (Source: Pathobiology)
Source: Pathobiology - January 16, 2019 Category: Pathology Source Type: research

Rapidly and Slowly Growing Lineages in Chromosomal Instability-Type Gland-Forming Gastric Carcinomas as Revealed by Multisampling Analysis of DNA Copy-Number Profile
Conclusions: We demonstrated that CIN-type GC could be categorized into 2 genetic lineages which are different in terms of rapidity of local extension but similar in terms of nodal metastasis risk.Pathobiology (Source: Pathobiology)
Source: Pathobiology - January 13, 2019 Category: Pathology Source Type: research

Three Novel < b > < i > MEN1 < /i > < /b > Variants in < b > < i > AIP < /i > < /b > -Negative Familial Isolated Pituitary Adenoma Patients
Conclusions:MEN1 alterations can be of significance in FIPA patients and screening could be offered toAIP mutation-negative patients without MEN1 features. Further studies are needed to clarify the role ofMEN1 in FIPA patients.Pathobiology (Source: Pathobiology)
Source: Pathobiology - January 13, 2019 Category: Pathology Source Type: research

Title Page / Table of Contents
Pathobiology 2019;86:1 –4 (Source: Pathobiology)
Source: Pathobiology - January 2, 2019 Category: Pathology Source Type: research

Advances in Bone Marrow Diagnostics of Patients with Cytopenia
Pathobiology (Source: Pathobiology)
Source: Pathobiology - November 26, 2018 Category: Pathology Source Type: research

Acknowledgement to Reviewers
Pathobiology 2018;85:348 (Source: Pathobiology)
Source: Pathobiology - November 14, 2018 Category: Pathology Source Type: research

Contents Vol. 85, 2018
Pathobiology 2018;85:1 –4 (Source: Pathobiology)
Source: Pathobiology - November 14, 2018 Category: Pathology Source Type: research

Extramedullary Haematopoiesis in Axillary Lymph Nodes of Breast Carcinoma Patients Receiving Neoadjuvant Chemotherapy: A Potential Diagnostic Pitfall
We report the case of a 68-year-old Caucasian female with a family history of cancer. Core biopsy revealed that she had grade II oestrogen receptor-negative, Her2-positive invasive ductal carcinoma. She was offered neoadjuvant chemotherapy with Herceptin and subsequently underwent breast-conserving surgery. Microscopic examination of the post-treatment breast surgical specimen showed a partial pathological response with large areas of tumour regression. The sentinel lymph node showed frequent large single and multinucleate giant cells with hyperchromatic nuclei located predominantly within the subcapsular and medullary sin...
Source: Pathobiology - November 13, 2018 Category: Pathology Source Type: research

Exosomes from Thymic Stromal Lymphopoietin-Activated Dendritic Cells Promote Th2 Differentiation through the OX40 Ligand
Conclusion: Blockade of OX40L in DC-derived exosomes could inhibit exosome-mediated CD4+ T proliferation and Th2 differentiation.Pathobiology (Source: Pathobiology)
Source: Pathobiology - November 8, 2018 Category: Pathology Source Type: research

Effect of Hawthorn Leaf Flavonoids in Dehydroepiandrosterone-Induced Polycystic Ovary Syndrome in Rats
Conclusion: HLF manifest protective effects against PCOS progression in the animal model, which may hold great promise for future clinical applications.Pathobiology (Source: Pathobiology)
Source: Pathobiology - November 7, 2018 Category: Pathology Source Type: research

< b > < i > RUNX1 < /i > < /b > Mutations Can Lead to Aberrant Expression of CD79a and PAX5 in Acute Myelogenous Leukemias: A Potential Diagnostic Pitfall
Conclusion: Aberrant CD79a and/or PAX5 expression can be found in AML cases withRUNX1 mutations even without the translocation t(8; 21). Our series shows the expression of CD79a and PAX5 to be a potential pitfall in the classification ofRUNX1 mutant acute leukemia.Pathobiology (Source: Pathobiology)
Source: Pathobiology - November 7, 2018 Category: Pathology Source Type: research

The American Joint Commission Cancer 8th Edition Prognostic Stage Including Oncotype DX ® Recurrence Score: Impact on Staging of Early Breast Cancer
Conclusion: Application of PS and RS-modified PS results in tumour downstaging in approximately 20% of patients with early BC. Upstaging was observed in 9% of patients when staged according to PS and was primarily due to the impact of high histological grade.Pathobiology (Source: Pathobiology)
Source: Pathobiology - October 24, 2018 Category: Pathology Source Type: research

Progression Potential of Ductal Carcinoma in situ Assessed by Genomic Copy Number Profiling
Conclusion: Classification of breast tumors into rapidly progressive cluster A and the other (cluster B) may contribute to select the treatment appropriate for their progression risk.Pathobiology (Source: Pathobiology)
Source: Pathobiology - October 17, 2018 Category: Pathology Source Type: research

Breast Tumours Resembling the Tall Cell Variant of Thyroid Papillary Carcinoma: Are They Part of the Papillary Carcinoma Spectrum or a Distinct Entity?
Conclusion: Features characteristic of BTRTPC overlap with other PCs of the breast. Molecular and immunohistochemical biomarkers are needed to provide objective diagnostic criteria for the characterisation of such lesions in routine practice.Pathobiology (Source: Pathobiology)
Source: Pathobiology - October 11, 2018 Category: Pathology Source Type: research

Resistance to Fas-Mediated Apoptosis Does Not Correlate to Structural Alterations or Expression Changes of the Death Receptor in Papillary Thyroid Carcinomas
Conclusions: Our results denote thatFAS death domain orTP53 DNA-binding domain mutations, down-regulation of FAS receptor expression, or expression ofFAS soluble isoform are not responsible for the seeming inhibition of FAS-mediated apoptosis in papillary thyroid carcinoma cells.Pathobiology (Source: Pathobiology)
Source: Pathobiology - October 5, 2018 Category: Pathology Source Type: research

Early Loss of Forkhead Transcription Factor, O Subgroup, Member 1 Protein in the Development of Pancreatic Ductal Adenocarcinoma
Conclusions: Loss of FOXO1 protein is identified as an early event during PDAC development and may be independent of the top 4 mutated cancer genes. Because of its strong expression in normal ductal cells, immunohistochemical detection of FOXO1 can function as a valuable test to establish the diagnosis of transformation and malignancy in pancreatic tissues.Pathobiology (Source: Pathobiology)
Source: Pathobiology - September 18, 2018 Category: Pathology Source Type: research

Clinical Implication of Multi-Parameter Flow Cytometry in Myelodysplastic Syndromes
Myelodysplastic syndromes (MDS) are a challenging group of diseases for clinicians and researchers, as both disease course and pathobiology are highly heterogeneous. In (suspected) MDS patients, multi-parameter flow cytometry can aid in establishing diagnosis, risk stratification and choice of therapy. This review addresses the developments and future directions of multi-parameter flow cytometry scores in MDS. Additionally, we propose an integrated diagnostic algorithm for suspected MDS.Pathobiology 2018;85:274 –283 (Source: Pathobiology)
Source: Pathobiology - September 18, 2018 Category: Pathology Source Type: research

Metastatic Prostate Carcinoma from Imperial Rome (1st to 2nd Centuries AD)
Conclusion: At present, this is the only case of prostate cancer from the Imperial Age recovered in Rome.Pathobiology (Source: Pathobiology)
Source: Pathobiology - September 17, 2018 Category: Pathology Source Type: research

Immunohistochemical and Molecular Analyses Focusing on Mesenchymal Cells in Papillary Thyroid Carcinoma with Desmoid-Type Fibromatosis
Conclusion: This study suggests the significance of theBRAFV600E mutation and activation of Wnt signaling pathway in the carcinoma cells and DTF components, respectively. We believe that theCTNNB1 mutations are not the major factor behind β-catenin translocation indicating Wnt pathway activation. Further study is required to evaluate whether molecular abnormalities other than theCTNNB1 mutation cause activation of Wnt signaling in DTF components of PTC-DTF.Pathobiology (Source: Pathobiology)
Source: Pathobiology - September 17, 2018 Category: Pathology Source Type: research

Differential Expression of Cancer-Associated Fibroblast-Related Proteins in Ductal Carcinoma in situ According to Molecular Subtype and Stromal Histology
Conclusion: The expression of CAF-related proteins in stromal cells of DCIS varies according to molecular subtype and stromal type.Pathobiology (Source: Pathobiology)
Source: Pathobiology - September 17, 2018 Category: Pathology Source Type: research