JWA gene based integrin α(v)β3 targeting polypeptide PJP1-RGD inhibits pancreatic ductal adenocarcinoma metastasis and dyshomeostasis in mice
Objectives The Pancreatic ductal adenocarcinoma (PDAC) are incurable due to the rapid development of resistance to all current therapeutics. In contrast to normal tissue, the majority of PDACs express the integrin receptor however the tumor suppressor JWA is inactivated. We investigated the role of JWA gene based integrin α(v)β3 targetting polypeptide PJP1-RGD on PDAC metastasis and dyshomeostasis in mice. (Source: Pancreatology)
Source: Pancreatology - June 1, 2018 Category: Gastroenterology Authors: Jiahua Cui, Zhangding Wang, Xiaoyin Wang, Junjie Chen, Zhiqiang Wang, Yanpeng Hao, Yanping Zhu, Chunmei Zhao, Duan Chen, Jianwei Zhou Tags: 9. Experimental pancreatic cancer I Source Type: research
Affinity proteomic profiling of plasma for proteins associated to pancreatic cancer reveal candidates for patient survival
Objectives We investigated the plasma proteomes of PDAC patients using a multiplexed antibody bead array with the aim to identify proteins that are organ-specific and associated to features of the disease. The candidate proteins could complement existing tools for early detection of PDAC, assessment of tumor stages, or predict patient survival. (Source: Pancreatology)
Source: Pancreatology - June 1, 2018 Category: Gastroenterology Authors: Tea Dodig-Crnkovic, Burcu Ayoglu, Mun-Gwan Hong, Annika Bendes, Claudia Fredolini, Mathias Uhl én, Matthias Löhr, Inna Chen, Julia S. Johansen, Jochen M. Schwenk Tags: 9. Experimental pancreatic cancer I Source Type: research
Characterization of pancreatic and biliary cancer stem cells in patient-derived tissue
Objectives Pancreatic ductal adenocarcinoma (PDAC) and extrahepatic cholangiocarcinoma (eCC) represent two of the most lethal cancer entities. Cancer stem cells (CSCs) have been identified as crucial for tumor growth, metastasis, chemoresistance and thus represent a potential therapeutic target. (Source: Pancreatology)
Source: Pancreatology - June 1, 2018 Category: Gastroenterology Authors: Rosa Schmuck, Julia Gogolok, Elisabeth Seidel, Anja Schirmeier, Andreas Andreou, Thomas Malinka, Fritz Klein, Johann Pratschke, Marcus Bahra Tags: 9. Experimental pancreatic cancer I Source Type: research
Effect of cathepsin inhibition on metabolism and polarization of tumor-associated macrophages
Objectives Pancreatic ductal adenocarcinoma (PDAC) is characterized by an extensive stroma reaction. Stroma-infiltrating immune cells such as tumor-associated macrophages (TAM) play an important role in regulating tumor progression and chemoresistance due to their production and secretion of numerous immune mediators including cathepsins. Accumulating evidence suggests that cathepsins play an important role as modulators of the TAM phenotype. (Source: Pancreatology)
Source: Pancreatology - June 1, 2018 Category: Gastroenterology Authors: Diana Oelschl ägel, Dana Reinicke, Tommy Weiss Sadan, Galia Blum, Almut Schulze, Patrick Michl Tags: 9. Experimental pancreatic cancer I Source Type: research
Establishment of light-based therapies to improve pancreatic cancer outcomes
Objectives Pancreatic cancer is one of the deadliest cancers and rates of incidence are steadily increasing. In current clinical trials major limitations in efficacy and toxicity remain. Hence, there is an urgent need to improve strategies for therapy. (Source: Pancreatology)
Source: Pancreatology - June 1, 2018 Category: Gastroenterology Authors: Pilar Acedo, Alexander Ney, Patricia Sancho, P ål Kristian Selbo, Alexander J. MacRobert, Stephen Pereira Tags: 9. Experimental pancreatic cancer I Source Type: research
Overall cellularity but not acellular stroma deposition increases gemcitabine accumulation in a genetically engineered mouse model of pancreatic cancer
Objectives The role of the activated stroma in PanIN-PDAC progression and in drug delivery is still a matter of debate. SPARC (Secreted Protein Acidic and Rich in Cysteine) has been implicated in collagen deposition and is predominantly expressed by cancer associated fibroblasts (CAFs). (Source: Pancreatology)
Source: Pancreatology - June 1, 2018 Category: Gastroenterology Authors: Iswarya Ramu, Robert Goetze, Melanie Patzak, Philipp Str öbel, Bence Sipos, Frances Richards, Duncan Jodrell, Elisabeth Hessmann, Volker Ellenrieder, Albrecht Neesse Tags: 9. Experimental pancreatic cancer I Source Type: research
The Characterization of “Islet Invasion” in pancreatic Ductal adenocarcinoma
Objectives Insulin and insulin-like growth factor (IGF) signaling axis prompts pancreatic ductal adenocarcinoma/PDAC into an advanced stage by enhancing tumor growth,metastasis and therapy resistance. In the pancreas tissue, the main source of insulin is the Langerhans ’ islets. Importantly, PDAC cells also invade islets during carcinogenesis and distort their structure, but this “islet invasion” has never been systematically characterized. (Source: Pancreatology)
Source: Pancreatology - June 1, 2018 Category: Gastroenterology Authors: Ayse Ceren Mutgan, Umut Calisan, Yusuf Ceyhun Erdogan, Helmut Friess, G üralp O. Ceyhan, Ihsan Ekin Demir Tags: 9. Experimental pancreatic cancer I Source Type: research
Small RNA sequencing of preoperative blood plasma identifies microRNA signature enabling to find pancreatic cancer patients who will not benefit from surgical resection
Objectives Pancreatic ductal adenocarcinoma (PDAC) is the most common and aggressive type of pancreatic cancer, associated with limited treatment options and high mortality rates. Radical resection remains the most effective curative approach for patients in early stage of disease. However, there is a high risk of surgical morbidity/mortality, and therefore development of prognostic assays to identify those patients who will not benefit from surgical resection, present important unmet medical need. (Source: Pancreatology)
Source: Pancreatology - June 1, 2018 Category: Gastroenterology Authors: Natalia Gablo, Vladim ír Procházka, Jan Hlavsa, Petr Moravčík, Tana Machackova, Zdeněk Kala, Jiri Sana, Parvez Ahmad, Ondrej Slaby Tags: 9. Experimental pancreatic cancer I Source Type: research
Are extracellular vesicles able to control human pluripotent stem cell fate?
Objectives Exosomes have been identified as mediators of cell-to-cell communication by delivering bioactive molecules.Cancer derived exosomes not only influence the invasive potentials of local neighboring cells, but also affect distant tissues.Induced pluripotent stem cells (iPSCs), obtained from adult somatic cells carry embryonic stem cells (ESC) properties, such as self renewal and ability to differentiate into all three embryonic germ layers.We asked whether the secretome of human pancreatic tumor cells selectively reprogram stem cells or progenitors to a pancreatic pre-malignant state. (Source: Pancreatology)
Source: Pancreatology - June 1, 2018 Category: Gastroenterology Authors: Etienne Buscail, Pascaline Quincy, Celine Gounou, Anne-Aurelie Raymond, Isabelle Lamrissi-Garcia, Isabelle Moranvillier, Frederic Saltel, Fran çois Moreau-Gaudry, Laurence Chiche, Aurelie Bedel, Alain Brisson, Sandrine Dabernat Tags: 9. Experimental pancreatic cancer I Source Type: research
Towards earlier detection of PDAC: Validation of a biomarker panel for the identification of type 3c diabetes among individuals newly diagnosed with type 2 diabetes
Objectives At the time of diagnosis over 40% of PDAC patients have diabetes mellitus (DM); up to 80% have glucose intolerance. The relationship between PDAC and DM is complex. Long-standing DM (LSDM) increases the risk of PDAC by approximately twofold. However, epidemiological data indicate that PDAC can cause DM, with DM diagnosis preceding cancer diagnosis by up to 3 years. In ∼50% of PDAC cases DM is of recent onset, making individuals with new-onset DM the largest high-risk group for PDAC. (Source: Pancreatology)
Source: Pancreatology - June 1, 2018 Category: Gastroenterology Authors: Lucy Oldfield, Rohith Rao, Tejpal Purewal, John P. Neoptolemos, William Greenhalf, Christopher Halloran, Eithne Costello Tags: 9. Experimental pancreatic cancer I Source Type: research
Inhibition of NF-kB decreases self-renewal in CD133 positive pancreatic cancer cells
Objectives Introduction: Pancreatic Cancer is a lethal malignancy with a dismissal 5-year survival rate of 7%. One of the main factors that makes pancreatic cancer a very difficult disease to treat is the presence of a sub population of cells, known as the cancer stem cells. These cancer stem cells have high expression of self-renewal genes namely SOX2, OCT4 and NANOG which confers them the ability to "self-renew". NF kB is a pro survival pathway which has been shown by numerous reports to be over expressed in Pancreatic Cancer. (Source: Pancreatology)
Source: Pancreatology - June 1, 2018 Category: Gastroenterology Authors: Nikita Sharma, Patricia Dauer, Vineet Kumar Gupta, Kousik Kumar Kesh, Roey Hadad, Vikas Dudeja, Ashok Saluja, Sulagna Banerjee Tags: 9. Experimental pancreatic cancer I Source Type: research
Kras downstream signaling in pancreatic ductal adenocarcinoma
Objectives To investigated the functions of oncogenic Kras downstream signaling pathways in pancreatic ductal adenocarcinoma (PDAC). Oncogenic Kras initiates the carcinogenesis via different signalling cascades including the PI3K-Pdk1 and the Raf-Mek-Erk pathway. We could show previously that the deletion of 3-phosphoinositide-dependet protein kinase 1 (Pdk1) completely blocks the formation of precursor lesions such as pancreatic intraepithelial lesions (PanIN) and PDAC in a pancreatic cancer mouse model. (Source: Pancreatology)
Source: Pancreatology - June 1, 2018 Category: Gastroenterology Authors: Hongkai Yan, Nina Sch önhuber, Christian Veltkamp, Barbara Seidler, Roland M. Schmid, Günter Schneider, Dieter Saur Tags: 9. Experimental pancreatic cancer I Source Type: research
Bile acids increase MUC4 expression in pancreatic ductal adenocarcinoma
Objectives Pancreatic cancer (PC) is the fourth leading cause of cancer death and accounts for 7% of all cancer death.Notably,70% of PC occurs in the head of the pancreas which reaching a critical size and can blocks the main pancreatic duct and the common bile duct,thus increasing the concentration of bile acids (BA's) in the serum.It is hypothesized that levels of BA's increases the tumorgenic potential of PC cells by changing the expression of oncogenic mucins. MUC4 is a trans-membrane glycoprotein that is overexpressed in pancreatic ductal adenocarcinoma (PDAC) but not in the normal pancreatic tissues. (Source: Pancreatology)
Source: Pancreatology - June 1, 2018 Category: Gastroenterology Authors: Eleon óra Gál, Attila Èbert, László Tiszlavicz, Viktória Venglovecz Tags: 9. Experimental pancreatic cancer I Source Type: research
RINT1 is essential to pancreas ductal adenocarcinoma (PDAC) homeostasis
Objectives RINT1 (RAD50-interacting protein 1) is a multifunctional protein playing a role in cell cycle regulation, genomic stability, telomere maintenance, ER-Golgi trafficking and autophagy. (Source: Pancreatology)
Source: Pancreatology - June 1, 2018 Category: Gastroenterology Authors: Frank Arnold, Ewa K. Kaminska, Johann Gout, Thomas Seufferlein, Lukas Perkhofer, Pierre-Olivier Frappart, Alexander Kleger Tags: 9. Experimental pancreatic cancer I Source Type: research
A novel protocol for isolation and culture of primary pancreatic stellate cells within the context of their tissue of origin
Objectives The contradicting evidence in scientific research thus far on the effect and the role of pancreatic stellate cells on pancreatic cancer cannot be ignored anymore. It is of no doubt that the stellate cells together with other cancer-associated fibroblasts are the main orchestrators of the unique desmoplastic reaction seen in the stroma of the pancreatic cancer. Since the identification and extraction of pancreatic stellate cells almost two decades ago, gathering evidence has been that they play a key role in promoting the proliferation invasion and spread of cancer cells in PDAC as well as provide a stem cell nic...
Source: Pancreatology - June 1, 2018 Category: Gastroenterology Authors: Mohammed Ghallab, Guglielmo Rosignoli, Cristina Ghirelli Tags: 9. Experimental pancreatic cancer I Source Type: research
