From osimertinib to preemptive combinations
This article also explores PCs for MET-driven lung cancer.PMID:38497774 | DOI:10.18632/oncotarget.28569 (Source: Oncotarget)
Source: Oncotarget - March 18, 2024 Category: Cancer & Oncology Authors: Mikhail V Blagosklonny Source Type: research

Correction: Sigma-1 and Sigma-2 receptor ligands induce apoptosis and autophagy but have opposite effect on cell proliferation in uveal melanoma
Oncotarget. 2024 Mar 14;15:199. doi: 10.18632/oncotarget.28519.NO ABSTRACTPMID:38484150 | PMC:PMC10939473 | DOI:10.18632/oncotarget.28519 (Source: Oncotarget)
Source: Oncotarget - March 14, 2024 Category: Cancer & Oncology Authors: Lucia Longhitano Carlo Castruccio Castracani Daniele Tibullo Roberto Avola Maria Viola Giuliano Russo Orazio Prezzavento Agostino Marrazzo Emanuele Amata Michele Reibaldi Antonio Longo Andrea Russo Nunziatina Laura Parrinello Giovanni Li Volti Source Type: research

G-quadruplex landscape and its regulation revealed by a new antibody capture method
We report a protocol that captures G4s from the cells efficiently without any bias as well as eliminates the detection of G4s formed artifactually on crosslinked sheared chromatin post-fixation. We discover that G4s form sparingly at SINEs. An application of this method shows that depletion of a repeat-binding protein CGGBP1 enhances net G4 capture at CGGBP1-dependent CTCF-binding sites and regions of sharp interstrand G/C-skew transitions. Thus, we present an improved method for G4 landscape determination and by applying it we show that sequence property-specific constraints of the nuclear environment mitigate G4 formatio...
Source: Oncotarget - March 14, 2024 Category: Cancer & Oncology Authors: Subhamoy Datta Manthan Patel Chakkarai Sathyaseelan Chandrama Ghosh Akanksha Mudgal Divyesh Patel Thenmalarchelvi Rathinavelan Umashankar Singh Source Type: research

Analytical validation of NeXT Personal ®, an ultra-sensitive personalized circulating tumor DNA assay
We describe the analytical validation of NeXT Personal®, an ultra-sensitive, tumor-informed circulating tumor DNA (ctDNA) assay for detecting residual disease, monitoring therapy response, and detecting recurrence in patients diagnosed with solid tumor cancers. NeXT Personal uses whole genome sequencing of tumor and matched normal samples combined with advanced analytics to accurately identify up to ~1,800 somatic variants specific to the patient's tumor. A personalized panel is created, targeting these variants and then used to sequence cell-free DNA extracted from patient plasma samples for ultra-sensitive detection of ...
Source: Oncotarget - March 14, 2024 Category: Cancer & Oncology Authors: Josette Northcott Gabor Bartha Jason Harris Conan Li Fabio C P Navarro Rachel Marty Pyke Manqing Hong Qi Zhang Shuyuan Ma Tina X Chen Janet Lai Nitin Udar Juan-Sebastian Saldivar Erin Ayash Joshua Anderson Jiang Li Tiange Cui Tu Le Ruthie Chow Randy Jerel Source Type: research

ABT199/venetoclax synergism with thiotepa enhances the cytotoxicity of fludarabine, cladribine and busulfan in AML cells
Oncotarget. 2024 Mar 14;15:220-231. doi: 10.18632/oncotarget.28563.ABSTRACTABT199/venetoclax, an inhibitor of the pro-survival BCL-2 protein, has improved AML treatment. Its efficacy in hematopoietic stem cell transplantation (HSCT), when combined with other chemotherapeutic drugs, has not been thoroughly investigated. The present study demonstrates the synergistic cytotoxicity of ABT199/venetoclax with the DNA alkylator thiotepa (Thio) in AML cells. Cleavage of Caspase 3, PARP1 and HSP90, as well as increased Annexin V positivity, suggest potent activation of apoptosis by this two-drug combination; increased levels of γ-...
Source: Oncotarget - March 14, 2024 Category: Cancer & Oncology Authors: Benigno C Valdez Bin Yuan David Murray Jeremy L Ramdial Uday Popat Yago Nieto Borje S Andersson Source Type: research

Correction: Sigma-1 and Sigma-2 receptor ligands induce apoptosis and autophagy but have opposite effect on cell proliferation in uveal melanoma
Oncotarget. 2024 Mar 14;15:199. doi: 10.18632/oncotarget.28519.NO ABSTRACTPMID:38484150 | PMC:PMC10939473 | DOI:10.18632/oncotarget.28519 (Source: Oncotarget)
Source: Oncotarget - March 14, 2024 Category: Cancer & Oncology Authors: Lucia Longhitano Carlo Castruccio Castracani Daniele Tibullo Roberto Avola Maria Viola Giuliano Russo Orazio Prezzavento Agostino Marrazzo Emanuele Amata Michele Reibaldi Antonio Longo Andrea Russo Nunziatina Laura Parrinello Giovanni Li Volti Source Type: research

G-quadruplex landscape and its regulation revealed by a new antibody capture method
We report a protocol that captures G4s from the cells efficiently without any bias as well as eliminates the detection of G4s formed artifactually on crosslinked sheared chromatin post-fixation. We discover that G4s form sparingly at SINEs. An application of this method shows that depletion of a repeat-binding protein CGGBP1 enhances net G4 capture at CGGBP1-dependent CTCF-binding sites and regions of sharp interstrand G/C-skew transitions. Thus, we present an improved method for G4 landscape determination and by applying it we show that sequence property-specific constraints of the nuclear environment mitigate G4 formatio...
Source: Oncotarget - March 14, 2024 Category: Cancer & Oncology Authors: Subhamoy Datta Manthan Patel Chakkarai Sathyaseelan Chandrama Ghosh Akanksha Mudgal Divyesh Patel Thenmalarchelvi Rathinavelan Umashankar Singh Source Type: research

Analytical validation of NeXT Personal ®, an ultra-sensitive personalized circulating tumor DNA assay
We describe the analytical validation of NeXT Personal®, an ultra-sensitive, tumor-informed circulating tumor DNA (ctDNA) assay for detecting residual disease, monitoring therapy response, and detecting recurrence in patients diagnosed with solid tumor cancers. NeXT Personal uses whole genome sequencing of tumor and matched normal samples combined with advanced analytics to accurately identify up to ~1,800 somatic variants specific to the patient's tumor. A personalized panel is created, targeting these variants and then used to sequence cell-free DNA extracted from patient plasma samples for ultra-sensitive detection of ...
Source: Oncotarget - March 14, 2024 Category: Cancer & Oncology Authors: Josette Northcott Gabor Bartha Jason Harris Conan Li Fabio C P Navarro Rachel Marty Pyke Manqing Hong Qi Zhang Shuyuan Ma Tina X Chen Janet Lai Nitin Udar Juan-Sebastian Saldivar Erin Ayash Joshua Anderson Jiang Li Tiange Cui Tu Le Ruthie Chow Randy Jerel Source Type: research

ABT199/venetoclax synergism with thiotepa enhances the cytotoxicity of fludarabine, cladribine and busulfan in AML cells
Oncotarget. 2024 Mar 14;15:220-231. doi: 10.18632/oncotarget.28563.ABSTRACTABT199/venetoclax, an inhibitor of the pro-survival BCL-2 protein, has improved AML treatment. Its efficacy in hematopoietic stem cell transplantation (HSCT), when combined with other chemotherapeutic drugs, has not been thoroughly investigated. The present study demonstrates the synergistic cytotoxicity of ABT199/venetoclax with the DNA alkylator thiotepa (Thio) in AML cells. Cleavage of Caspase 3, PARP1 and HSP90, as well as increased Annexin V positivity, suggest potent activation of apoptosis by this two-drug combination; increased levels of γ-...
Source: Oncotarget - March 14, 2024 Category: Cancer & Oncology Authors: Benigno C Valdez Bin Yuan David Murray Jeremy L Ramdial Uday Popat Yago Nieto Borje S Andersson Source Type: research

Correction: Sigma-1 and Sigma-2 receptor ligands induce apoptosis and autophagy but have opposite effect on cell proliferation in uveal melanoma
Oncotarget. 2024 Mar 14;15:199. doi: 10.18632/oncotarget.28519.NO ABSTRACTPMID:38484150 | DOI:10.18632/oncotarget.28519 (Source: Oncotarget)
Source: Oncotarget - March 14, 2024 Category: Cancer & Oncology Authors: Lucia Longhitano Carlo Castruccio Castracani Daniele Tibullo Roberto Avola Maria Viola Giuliano Russo Orazio Prezzavento Agostino Marrazzo Emanuele Amata Michele Reibaldi Antonio Longo Andrea Russo Nunziatina Laura Parrinello Giovanni Li Volti Source Type: research

G-quadruplex landscape and its regulation revealed by a new antibody capture method
We report a protocol that captures G4s from the cells efficiently without any bias as well as eliminates the detection of G4s formed artifactually on crosslinked sheared chromatin post-fixation. We discover that G4s form sparingly at SINEs. An application of this method shows that depletion of a repeat-binding protein CGGBP1 enhances net G4 capture at CGGBP1-dependent CTCF-binding sites and regions of sharp interstrand G/C-skew transitions. Thus, we present an improved method for G4 landscape determination and by applying it we show that sequence property-specific constraints of the nuclear environment mitigate G4 formatio...
Source: Oncotarget - March 14, 2024 Category: Cancer & Oncology Authors: Subhamoy Datta Manthan Patel Chakkarai Sathyaseelan Chandrama Ghosh Akanksha Mudgal Divyesh Patel Thenmalarchelvi Rathinavelan Umashankar Singh Source Type: research

Analytical validation of NeXT Personal ®, an ultra-sensitive personalized circulating tumor DNA assay
We describe the analytical validation of NeXT Personal®, an ultra-sensitive, tumor-informed circulating tumor DNA (ctDNA) assay for detecting residual disease, monitoring therapy response, and detecting recurrence in patients diagnosed with solid tumor cancers. NeXT Personal uses whole genome sequencing of tumor and matched normal samples combined with advanced analytics to accurately identify up to ~1,800 somatic variants specific to the patient's tumor. A personalized panel is created, targeting these variants and then used to sequence cell-free DNA extracted from patient plasma samples for ultra-sensitive detection of ...
Source: Oncotarget - March 14, 2024 Category: Cancer & Oncology Authors: Josette Northcott Gabor Bartha Jason Harris Conan Li Fabio C P Navarro Rachel Marty Pyke Manqing Hong Qi Zhang Shuyuan Ma Tina X Chen Janet Lai Nitin Udar Juan-Sebastian Saldivar Erin Ayash Joshua Anderson Jiang Li Tiange Cui Tu Le Ruthie Chow Randy Jerel Source Type: research

ABT199/venetoclax synergism with thiotepa enhances the cytotoxicity of fludarabine, cladribine and busulfan in AML cells
Oncotarget. 2024 Mar 14;15:220-231. doi: 10.18632/oncotarget.28563.ABSTRACTABT199/venetoclax, an inhibitor of the pro-survival BCL-2 protein, has improved AML treatment. Its efficacy in hematopoietic stem cell transplantation (HSCT), when combined with other chemotherapeutic drugs, has not been thoroughly investigated. The present study demonstrates the synergistic cytotoxicity of ABT199/venetoclax with the DNA alkylator thiotepa (Thio) in AML cells. Cleavage of Caspase 3, PARP1 and HSP90, as well as increased Annexin V positivity, suggest potent activation of apoptosis by this two-drug combination; increased levels of γ-...
Source: Oncotarget - March 14, 2024 Category: Cancer & Oncology Authors: Benigno C Valdez Bin Yuan David Murray Jeremy L Ramdial Uday Popat Yago Nieto Borje S Andersson Source Type: research

Correction: Sigma-1 and Sigma-2 receptor ligands induce apoptosis and autophagy but have opposite effect on cell proliferation in uveal melanoma
Oncotarget. 2024 Mar 14;15:199. doi: 10.18632/oncotarget.28519.NO ABSTRACTPMID:38484150 | DOI:10.18632/oncotarget.28519 (Source: Oncotarget)
Source: Oncotarget - March 14, 2024 Category: Cancer & Oncology Authors: Lucia Longhitano Carlo Castruccio Castracani Daniele Tibullo Roberto Avola Maria Viola Giuliano Russo Orazio Prezzavento Agostino Marrazzo Emanuele Amata Michele Reibaldi Antonio Longo Andrea Russo Nunziatina Laura Parrinello Giovanni Li Volti Source Type: research

G-quadruplex landscape and its regulation revealed by a new antibody capture method
We report a protocol that captures G4s from the cells efficiently without any bias as well as eliminates the detection of G4s formed artifactually on crosslinked sheared chromatin post-fixation. We discover that G4s form sparingly at SINEs. An application of this method shows that depletion of a repeat-binding protein CGGBP1 enhances net G4 capture at CGGBP1-dependent CTCF-binding sites and regions of sharp interstrand G/C-skew transitions. Thus, we present an improved method for G4 landscape determination and by applying it we show that sequence property-specific constraints of the nuclear environment mitigate G4 formatio...
Source: Oncotarget - March 14, 2024 Category: Cancer & Oncology Authors: Subhamoy Datta Manthan Patel Chakkarai Sathyaseelan Chandrama Ghosh Akanksha Mudgal Divyesh Patel Thenmalarchelvi Rathinavelan Umashankar Singh Source Type: research