The origins of nitric oxide and peroxynitrite research in Uruguay: 25 years of contributions to the biochemical and biomedical sciences
Publication date: Available online 11 March 2019Source: Nitric OxideAuthor(s): Rafael RadiAbstractIn this review, I provide historical background on how nitric oxide (•NO) and peroxynitrite research was originated in Uruguay in the early 90′s and how the investigations evolved through over more than two decades within a context related to human biology. This process involved the participation of multiple local investigators, in conjunction with collaborations at the regional (Latin American) and international levels. The discoveries have been integrated with parallel investigations from other research groups worldwide ...
Source: Nitric Oxide - March 12, 2019 Category: Chemistry Source Type: research

Increase of cystathionine-γ-lyase (CSE) during late wound repair: Hydrogen sulfide triggers cytokeratin 10 expression in keratinocytes
Publication date: Available online 9 March 2019Source: Nitric OxideAuthor(s): Itamar Goren, Yvette Köhler, Ahmed Aglan, Josef Pfeilschifter, Karl-Friedrich Beck, Stefan FrankAbstractThe gaseous mediators nitric oxide (NO), carbon monoxide (CO) and lately also hydrogen sulfide (H2S) have been described to contribute to the interplay of protein type- and lipid mediators in the regulation of wound healing. In particular, the recently reported role of H2S in skin repair remains largely unresolved. Therefore we assessed the expressional kinetics of potential H2S-producing enzymes during undisturbed skin repair: the cystathioni...
Source: Nitric Oxide - March 10, 2019 Category: Chemistry Source Type: research

S-nitrosylation and its role in breast cancer angiogenesis and metastasis
Publication date: Available online 9 March 2019Source: Nitric OxideAuthor(s): Pamela Ehrenfeld, Francisco Cordova, Walter N. Duran, Fabiola A. SanchezAbstractS-nitrosylation, the modification by nitric oxide of free sulfhydryl groups in cysteines, has become an important regulatory mechanism in carcinogenesis and metastasis. S-nitrosylation of targets in tumor cells contributes to metastasis regulating epithelial to mesenchymal transition, migration and invasion. In the tumor environment, the role of S-nitrosylation in endothelium has not been addressed; however, the evidence points out that S-nitrosylation of endothelial ...
Source: Nitric Oxide - March 9, 2019 Category: Chemistry Source Type: research

The oral nitrate-reducing capacity correlates with peak power output and peak oxygen uptake in healthy humans
In conclusion, this is the first evidence showing a link between the oral nitrate-reducing capacity and markers of aerobic fitness levels in healthy humans. (Source: Nitric Oxide)
Source: Nitric Oxide - March 8, 2019 Category: Chemistry Source Type: research

The role of NO-cGMP pathway inhibition in vascular endothelial-dependent smooth muscle relaxation disorder of AT1-AA positive rats: Protective effects of adiponectin
Publication date: Available online 1 March 2019Source: Nitric OxideAuthor(s): Zhiyuan Wang, Ye Wu, Suli Zhang, Yuhui Zhao, Xiaochen Yin, Wen Wang, Xinliang Ma, Huirong LiuAbstractAngiotensin II type 1 receptor autoantibodies (AT1-AA) cause endothelial-dependent smooth muscle relaxation disorder. It is well understood that impairment of the NO–cGMP signaling pathway is one of the mechanisms of endothelial-dependent smooth muscle relaxation disorder. However, it is still unclear whether AT1-AA induces endothelial-dependent smooth muscle relaxation disorder via the impairment of the NO–cGMP signaling pathway. In addition,...
Source: Nitric Oxide - March 5, 2019 Category: Chemistry Source Type: research

Exercise training restores eNOS activation in the perivascular adipose tissue of obese rats: Impact on vascular function
ConclusionExercise training in obese rats is able to impact PVAT eNOS with subsequent beneficial impact on vascular function.Graphical abstract (Source: Nitric Oxide)
Source: Nitric Oxide - March 5, 2019 Category: Chemistry Source Type: research

The active-site cysteine residue of Ca2+/calmodulin-dependent protein kinase I is protected from irreversible modification via generation of polysulfidation
Publication date: Available online 4 March 2019Source: Nitric OxideAuthor(s): Tsuyoshi Takata, Ayaka Tsukuda, Yukihiro Tsuchiya, Takaaki Akaike, Yasuo WatanabeAbstractCa2+/calmodulin (CaM)-dependent protein kinase (CaMK) I is activated by the phosphorylation of a crucial activation loop Thr177 by upstream kinases, CaMK kinase (CaMKK), and regulates axonal or dendritic extension and branching. Reactive sulfur species (RSS) modulate protein functions via polysulfidation of the reactive Cys residues. Here, we report that the activity of CaMKI was reversibly inhibited via its polysulfidation at Cys179 by RSS. In vitro incubati...
Source: Nitric Oxide - March 5, 2019 Category: Chemistry Source Type: research

Sodium/calcium exchanger is involved in apoptosis induced by H2S in tumor cells through decreased levels of intracellular pH
Publication date: Available online 5 March 2019Source: Nitric OxideAuthor(s): Ivan Szadvari, Sona Hudecova, Barbora Chovancova, Miroslava Matuskova, Dana Cholujova, Lubomira Lencesova, David Valerian, Karol Ondrias, Petr Babula, Olga KrizanovaAbstractWe explored possibility that sodium/calcium exchanger 1 (NCX1) is involved in pH modulation and apoptosis induction in GYY4137 treated cells. We have shown that although 10 days treatment with GYY4137 did not significantly decreased volume of tumors induced by colorectal cancer DLD1 cells in nude mice, it already induced apoptosis in these tumors. Treatment of DLD1 and ovarian...
Source: Nitric Oxide - March 5, 2019 Category: Chemistry Source Type: research

Nitration of hIAPP promotes its toxic oligomer formation and exacerbates its toxicity towards INS-1 cells
In this study, fluorescent assays, transmission electron microscopy (TEM), atomic force microscope (AFM) were used to demonstrate that nitration of hIAPP significantly decreased its fibril formation. But the decreased fibril formation was not through the diminished aggregation of hIAPP monomer as suggested by the results of circular dichroism spectroscopy (CD) and gel electrophoresis assay. Surface-enhanced raman spectroscopy (SERS) indicated that nitration of hIAPP impaired the intermolecular hydrogen bonding. On the basis of these results, we hypothesize that nitration of hIAPP may block the intermolecular hydrogen bondi...
Source: Nitric Oxide - March 5, 2019 Category: Chemistry Source Type: research

Cx46 hemichannel modulation by nitric oxide: Role of the fourth transmembrane helix cysteine and its possible involvement in cataract formation
Publication date: Available online 21 February 2019Source: Nitric OxideAuthor(s): Mauricio A. Retamal, Viviana P. Orellana, Nicolás J. Arévalo, Cristóbal G. Rojas, Rodolfo J. Arjona, Constanza A. Alcaíno, Wendy González, Jonathan G. Canan, Rodrigo Moraga-Amaro, Jimmy Stehberg, Luis Reuss, Guillermo A. AltenbergAbstractUnder normal conditions, connexin (Cx) hemichannels have a low open probability, which can increase under pathological conditions. Since hemichannels are permeable to relatively large molecules, their exacerbated activity has been linked to cell damage. Cx46 is highly expressed in the lens and its mutati...
Source: Nitric Oxide - February 22, 2019 Category: Chemistry Source Type: research

Editorial Board
Publication date: 1 April 2019Source: Nitric Oxide, Volume 85Author(s): (Source: Nitric Oxide)
Source: Nitric Oxide - February 22, 2019 Category: Chemistry Source Type: research

Cytotoxicity, cellular uptake, and subcellular localization of a nitrogen oxide and aminopropyl-β-lactose derivative ruthenium complex used as nitric oxide delivery agent
Publication date: Available online 19 February 2019Source: Nitric OxideAuthor(s): Joicy S. dos Santos, Loyanne C. Ramos, Lucimara P. Ferreira, Vanessa Campos, Lucas C.D. de Rezende, Flávio da Silva Emery, Roberto Santana da SilvaAbstractThis work investigates how the luminescent ruthenium-nitrite complexes cis-[Ru(py-bodipy)(dcbpy)2(NO2)](PF6) (I) and cis-[Ru(py-bodipy)(dcbpy-aminopropyl-β-lactose)2(NO2)](PF6) (II) behave toward the melanoma cancer cell line B16F10. The chemical structure and purity of the synthesized complexes were analyzed by UV-Visible and FTIR spectroscopy, MALDI, HPLC, and 1H NMR. Spectrofluorescenc...
Source: Nitric Oxide - February 19, 2019 Category: Chemistry Source Type: research

The effect of passive smoking on exhaled nitric oxide in asthmatic children
Publication date: Available online 16 February 2019Source: Nitric OxideAuthor(s): M. Bobrowska-Korzeniowska, I. Stelmach, A. Brzozowska, J. Jerzyńska, M. Mitał, W. StelmachAbstractTo date, some studies suggest that passive smoking (PS) may be an important determinant of FeNO levels in children but sill there is a need of investigations using objective methods of PS exposure.The aim of our study was to examine the effect of PS, measured by urine cotinine levels, on FeNO and lung function (FEV1) in allergic and non-allergic asthmatic children.MethodsIt was a prospective, non-interventional study. 140 children, aged 4–17,...
Source: Nitric Oxide - February 17, 2019 Category: Chemistry Source Type: research

NO donors induce vascular relaxation by different cellular mechanisms in hypertensive and normotensive rats
ConclusionOur results indicate that SNP and RuBPY-induced mesenteric resistance artery relaxation involves NO/sGC/cGMP/PKG pathway activation. K+ channels and SERCA activation is required to SNP but not for RuBPY-induced relaxation. Moreover, SERCA seems to be impaired in hypertensive 2K-1C rat mesenteric resistance arteries although it does not impact SNP- or RuBPY-induced relaxation.Graphical abstract (Source: Nitric Oxide)
Source: Nitric Oxide - February 15, 2019 Category: Chemistry Source Type: research

Cross-talk between the Ras GTPase and the Hog1 survival pathways in response to nitrosative stress in Paracoccidioides brasiliensis
In this report, we investigated the role of Ras GTPase in P. brasiliensis after in vitro stimulus with nitric oxide (NO). We observed that low concentrations of NO induced cell proliferation in P. brasiliensis, while high concentrations promoted decrease in fungal viability, and both events were reversed in the presence of a NO scavenger. We observed that high levels of NO induced Ras activation and its S-nitrosylation. Additionally, we showed that Ras modulated the expression of antioxidant genes in response to nitrosative stress. We find that the Hog1 MAP kinase contributed to nitrosative stress response in P. brasiliens...
Source: Nitric Oxide - February 15, 2019 Category: Chemistry Source Type: research