Sickle cell disease subjects and mouse models have elevated nitrite and cGMP levels in blood compartments
Publication date: Available online 2 November 2019Source: Nitric OxideAuthor(s): Luis E.F. Almeida, Sayuri Kamimura, Celia M. de Souza Batista, Nicholas Spornick, Margaret Y. Nettleton, Elizabeth Walek, Meghann L. Smith, Julia Finkel, Deepika Darbari, Paul Wakim, Zenaide M.N. QuezadoAbstractThe hypothesis of decreased nitric oxide (NO) bioavailability in sickle cell disease (SCD) proposes that multiple factors leading to decreased NO production and increased consumption contributes to vaso-occlusion, pulmonary hypertension, and pain. The anion nitrite is central to NO physiology as it is an end product of NO metabolism and...
Source: Nitric Oxide - November 3, 2019 Category: Chemistry Source Type: research
Effects of age, sex and diet on salivary nitrate and nitrite in infants
Publication date: Available online 2 November 2019Source: Nitric OxideAuthor(s): Niklas Timby, Magnus Domellöf, Olle Hernell, Bo Lönnerdal, Carina Nihlen, Ingegerd Johanssson, Eddie WeitzbergAbstractThe inorganic anions nitrate and nitrite are oxidation products from endogenous nitric oxide (NO) generation and constituents in our diet. A nitrate-nitrite-NO pathway exists in which nitrate can be serially reduced to bioactive NO. The first step of this pathway occurs in the oral cavity where oral bacteria convert salivary nitrate to nitrite, whereafter nitrite is reduced to NO systemically by several enzymatic and non-enzy...
Source: Nitric Oxide - November 3, 2019 Category: Chemistry Source Type: research
Influence of hemoglobin and albumin on the NO donation effect of tetranitrosyl iron complex with thiosulfate
Publication date: Available online 31 October 2019Source: Nitric OxideAuthor(s): Olesya Pokidova, Tatiana Rudneva, Bogdan Tretyakov, Raisa Kotelnikova, Alexander Kotelnikov, Sergey AldoshinAbstractThe effects of deoxyhemoglobin (Hb) and albumin on the NO-donor activity of the anionic tetranitrosyl iron complex with thiosulfate ligands (1) were studied for the first time. It was shown that Hb significantly stabilizes complex 1; in its presence, NO generation from the complex proceeds at a noticeably slower rate. A similar effect is observed when complex 1 is bound to albumin, in which case complex 1 decomposes 27 times slow...
Source: Nitric Oxide - November 1, 2019 Category: Chemistry Source Type: research
A randomized pilot study of nitrate supplementation with beetroot juice in acute respiratory failure
Publication date: Available online 25 October 2019Source: Nitric OxideAuthor(s): D. Clark Files, Timothy Heinrich, Katherine L. Shields, Nathan J. Love, Carly Brailer, Rita N. Bakhru, Lina Purcell, Lori Flores, Kevin Gibbs, Gary D. Miller, Peter E. Morris, Michael J. BerryAbstractNitrate rich beetroot juice (BRJ) can enhance nitric oxide signaling, leading to improved physical function in healthy and diseased populations, but its safety and biologic efficacy have not been evaluated in a critically ill population. We randomized 22 previously functional acute respiratory failure patients to either BRJ or placebo daily until ...
Source: Nitric Oxide - October 26, 2019 Category: Chemistry Source Type: research
Modulation of endothelium-derived nitric oxide production and activity by taurine and taurine-conjugated bile acids
Publication date: Available online 24 October 2019Source: Nitric OxideAuthor(s): Daniele M. Guizoni, Jean F. Vettorazzi, Everardo M. Carneiro, Ana Paula DavelAbstractTaurine is a semiessential amino acid found at high concentrations in mammalian plasma and cells, where it regulates cellular functions such as ion flux, controls cell volume and serves as a substrate for conjugated bile acids (BAs). Exogenous administration of both taurine and taurine-conjugated BAs have also been implicated in the modulation of cardiovascular functions. This brief review summarizes the role of taurine and taurine-conjugated BAs in vascular r...
Source: Nitric Oxide - October 25, 2019 Category: Chemistry Source Type: research
Central angiotensin II-Protein inhibitor of neuronal nitric oxide synthase (PIN) axis contribute to neurogenic hypertension
Publication date: Available online 22 October 2019Source: Nitric OxideAuthor(s): Neeru M. Sharma, Andrea S. Haibara, Kenichi Katsurada, Xuefei Liu, Kaushik P. PatelAbstractActivation of renin-angiotensin- system, nitric oxide (NO•) bioavailability and subsequent sympathoexcitation plays a pivotal role in the pathogenesis of many cardiovascular diseases, including hypertension. Previously we have shown increased protein expression of PIN (a protein inhibitor of nNOS: neuronal nitric oxide synthase, known to dissociate nNOS dimers into monomers) with concomitantly reduced levels of catalytically active dimers of nNOS in th...
Source: Nitric Oxide - October 23, 2019 Category: Chemistry Source Type: research
Treatment with nitrite prevents reactive oxygen species generation in the corpora cavernosa and restores intracavernosal pressure in hypertensive rats
Publication date: Available online 11 October 2019Source: Nitric OxideAuthor(s): Natália A. Gonzaga, Gabriel T. do Vale, Carla B.P. da Silva, Lucas C. Pinheiro, Letícia N. Leite, Fernando S. Carneiro, José E. Tanus-Santos, Carlos R. TirapelliAbstractHypertension is a risk factor for erectile dysfunction (ED) and both conditions are associated with oxidative stress. Given that nitrite is described to display antioxidant effects, we hypothesized that treatment with nitrite would exert antioxidant effects attenuating both reactive oxygen species (ROS) generation in the corpora cavernosa (CC) and ED induced by hypertension....
Source: Nitric Oxide - October 12, 2019 Category: Chemistry Source Type: research
Propargylglycine decreases neuro-immune interaction inducing pain response in temporomandibular joint inflammation model
Publication date: Available online 8 October 2019Source: Nitric OxideAuthor(s): Emanuela G. Garattini, Bruna M. Santos, Daniele Ferrari, Camila Porto, Heloísa D.C. Francescato, Terezila M. Coimbra, Christie R.A. Leite-Panissi, Luiz G.S. Branco, Glauce C. NascimentoAbstractThe mechanisms underlying temporomandibular disorders following orofacial pain remain unclear. Hydrogen sulfide (H2S), a newly identified gasotransmitter, has been reported to modulate inflammation. Cystathionine γ-lyase (CSE) is responsible for the systemical production of H2S, which exerts both pro- and antinociceptive effects through inflammation. In...
Source: Nitric Oxide - October 10, 2019 Category: Chemistry Source Type: research
Sapropterin reduces coronary artery malformation in offspring of pregestational diabetes mice
In conclusion, sapropterin treatment increases eNOS activity, reduces oxidative stress and prevents coronary artery malformation in offspring of pregestational diabetes. Sapropterin may have therapeutic potential in preventing coronary artery malformation in maternal diabetes.Graphical abstractOral sapropterin (Kuvan®) treatment inhibits oxidative stress, improves Akt/eNOS activity, and prevents coronary artery malformation (CAM) during pregestational diabetes. (Source: Nitric Oxide)
Source: Nitric Oxide - October 9, 2019 Category: Chemistry Source Type: research
Effects of dietary nitrate supplementation on microvascular physiology at 4559 m altitude – A randomised controlled trial (Xtreme Alps)
In conclusion, dietary nitrate supplementation does not improve microcirculatory function at 4559 m. (Source: Nitric Oxide)
Source: Nitric Oxide - October 9, 2019 Category: Chemistry Source Type: research
Nitrate uptake and metabolism in human skeletal muscle cell cultures
Publication date: Available online 8 October 2019Source: Nitric OxideAuthor(s): Sirada Srihirun, Ji Won Park, Rujia Teng, Waritta Sawaengdee, Barbora Piknova, Alan N. SchechterAbstractSeveral studies show that dietary nitrate enhances exercise performance, presumably by increasing muscle blood flow and improving oxygen utilization. These effects are likely mediated by nitrate metabolites, including nitrite and nitric oxide (NO). However, the mechanisms of nitrate production, storage, and metabolism to nitrite and NO in skeletal muscle cells are still unclear. We hypothesized that exogenous nitrate can be taken up and metab...
Source: Nitric Oxide - October 9, 2019 Category: Chemistry Source Type: research
Systemic vasoprotection by inhaled carbon monoxide is mediated through prolonged alterations in monocyte/macrophage function
Publication date: Available online 5 October 2019Source: Nitric OxideAuthor(s): Andrew Leake, Karim Salem, Michael C. Madigan, Ghee Rye Lee, Ankur Shukla, Guiying Hong, Brian S. Zuckerbraun, Edith TzengAbstractCarbon monoxide (CO) is anti-inflammatory and protective in models of disease. Its actions in vitro are short-lived but are sustained in vivo. We hypothesize that systemic CO can mediate prolonged phenotype changes in vivo, with a focus on macrophages (Mϕs). Mϕs isolated from CO treated rats responded to lipopolysaccharide (LPS) with increased IL6, IL10 and iNOS expression but decreased TNF. Conditioned media (CM) ...
Source: Nitric Oxide - October 6, 2019 Category: Chemistry Source Type: research
Inhibition of breast tumor growth by N(G)-nitro-l-arginine methyl ester (l-NAME) is accompanied by activation of fibroblasts
Publication date: Available online 19 September 2019Source: Nitric OxideAuthor(s): Marianela Sciacca, Denise Belgorosky, Macarena Zambrano, José Ignacio Gomez Escalante, Fernanda Roca, Yanina V. Langle, Eduardo O. Sandes, Catalina Lodillinsky, Ana María EijánAbstractNitric Oxide (NO) is involved in many physiological and pathological processes. It is generated by a family of NO synthases (NOS), being the inducible isoform, iNOS, responsible for higher amounts of NO. Here, we report that pharmacological inhibition of NO production by l-NAME reduces both viability and MAPK activated signalling pathways in iNOS positive hu...
Source: Nitric Oxide - September 21, 2019 Category: Chemistry Source Type: research
A forty year journey: The generation and roles of NO in plants
Publication date: Available online 18 September 2019Source: Nitric OxideAuthor(s): Zs Kolbert, J.B. Barroso, R. Brouquisse, F.J. Corpas, K.J. Gupta, C. Lindermayr, G.J. Loake, J.M. Palma, M. Petřivalský, D. Wendehenne, J.T. HancockAbstractIn this year there is the 40th anniversary of the first publication of plant nitric oxide (NO) emission by Lowell Klepper. In the decades since then numerous milestone discoveries have revealed that NO is a multifunctional molecule in plant cells regulating both plant development and stress responses. Apropos of the anniversary, these authors aim to review and discuss the developments o...
Source: Nitric Oxide - September 19, 2019 Category: Chemistry Source Type: research
Nitric oxide and its derivatives in the cancer battlefield
We present here the dual face of nitric oxide and its derivatives in cancer biology. Main derivatives of nitric oxide, such as nitrogen dioxide and peroxynitrite cause cell death by inducing protein and lipid peroxidation and/or DNA damage. Moreover, they control the activity of important protein players within the pro- and anti-apoptotic signaling pathways. Thus, the control of intracellular reactive nitrogen species may become a sophisticated tool in anticancer strategies. (Source: Nitric Oxide)
Source: Nitric Oxide - September 19, 2019 Category: Chemistry Source Type: research
