Cultured Transformed Human Podocytes
These podocytes are obtained from human patients and have been transformed by laboratory viruses so that they are immortal. The SV40 T antigen that is used to transform the cells is temperature-sensitive and so at 37 ˚C it is not active. The cells proliferate at 33 C and differentiate at 37 C. Cells from individuals with and without APOL1 renal risk genetic variants are available. (Source: NIDDK Research Materials)
Source: NIDDK Research Materials - September 4, 2018 Category: Research Source Type: research
Mice with Natural Gastrointestinal Microbiota
Among 21 distinct mouse populations worldwide, we identified a closely related wild relative to standard laboratory mouse strains. Its bacterial gut microbiome differed significantly from its laboratory mouse counterpart and was transferred to and maintained in laboratory mice over several generations. Laboratory mice reconstituted with natural microbiota exhibited reduced inflammation and increased survival following influenza virus infection and improved resistance against mutagen/inflammation-induced colorectal tumorigenesis. This model should enable the discovery of mechanisms that are relevant for host fitness and pro...
Source: NIDDK Research Materials - August 28, 2018 Category: Research Source Type: research
Miniature System for Manipulating Small Animals in High-Throughput Screening Small Molecules
Whole Animal Feeding Flat (WAFFL): A High-throughput Platform for the Automated Handling of FliesDrosophila is well-suited organism to be use in high-throughput studies due to their short, defined life cycle and genetic tools. However, current high-throughput fly methodologies are not ideal. They can require a significant amount of fly manipulation, the use of anesthetics, and a large quantity of drug or materials.Inventors at the National Institute of Diabetes and Digestive and Kidney Diseases of the National Institutes of Health have developed an elegant solution. This 96-well system called the Whole Animal Feeding Flat ...
Source: NIDDK Research Materials - August 22, 2018 Category: Research Source Type: research
Alb-tTA (Tg(Alb1-tTA)3123Lng) Mouse
Tetracycline-responsive transcriptional activator driven by the liver-specific mouse albumin promoter (Alb-tTA).TheE. Coli tetracycline operon regulatory system was used to generate a liver-specific transcription activation system that was inhibited by tetracycline. The transcription activator was a fused protein consisting of a tetracycline repressor gene (tetR) that was only active in the presence of tetracycline and a herpes simplex virus protein (VP-16) transcription activating domain. Transcription was induced only in the absence of tetracycline (Tet-Off). A liver-specific promoter such as mouse albumin determined tha...
Source: NIDDK Research Materials - August 20, 2018 Category: Research Source Type: research
