A paradigm for ethanol consumption in head-fixed mice during prefrontal cortical two-photon calcium imaging
Neuropharmacology. 2023 Dec 4:109800. doi: 10.1016/j.neuropharm.2023.109800. Online ahead of print.ABSTRACTThe prefrontal cortex (PFC) is a hub for cognitive behaviors and is a key target for neuroadaptations in alcohol use disorders. Recent advances in genetically encoded sensors and functional microscopy allow multimodal in-vivo PFC activity recordings at subcellular and cellular scales. While these methods could enable a deeper understanding of the relationship between alcohol and PFC function/dysfunction, they typically require animals to be head-fixed. Here, we present a method in mice for binge-like ethanol consumpti...
Source: Neuropharmacology - December 6, 2023 Category: Drugs & Pharmacology Authors: Anagha Kalelkar Grayson Sipe Ana Raquel Castro E Costa Ilka M Lorenzo My Nguyen Ivan Linares-Garcia Elena Vazey Rafiq Huda Source Type: research

A paradigm for ethanol consumption in head-fixed mice during prefrontal cortical two-photon calcium imaging
Neuropharmacology. 2023 Dec 4:109800. doi: 10.1016/j.neuropharm.2023.109800. Online ahead of print.ABSTRACTThe prefrontal cortex (PFC) is a hub for cognitive behaviors and is a key target for neuroadaptations in alcohol use disorders. Recent advances in genetically encoded sensors and functional microscopy allow multimodal in-vivo PFC activity recordings at subcellular and cellular scales. While these methods could enable a deeper understanding of the relationship between alcohol and PFC function/dysfunction, they typically require animals to be head-fixed. Here, we present a method in mice for binge-like ethanol consumpti...
Source: Neuropharmacology - December 6, 2023 Category: Drugs & Pharmacology Authors: Anagha Kalelkar Grayson Sipe Ana Raquel Castro E Costa Ilka M Lorenzo My Nguyen Ivan Linares-Garcia Elena Vazey Rafiq Huda Source Type: research

A paradigm for ethanol consumption in head-fixed mice during prefrontal cortical two-photon calcium imaging
Neuropharmacology. 2023 Dec 4:109800. doi: 10.1016/j.neuropharm.2023.109800. Online ahead of print.ABSTRACTThe prefrontal cortex (PFC) is a hub for cognitive behaviors and is a key target for neuroadaptations in alcohol use disorders. Recent advances in genetically encoded sensors and functional microscopy allow multimodal in-vivo PFC activity recordings at subcellular and cellular scales. While these methods could enable a deeper understanding of the relationship between alcohol and PFC function/dysfunction, they typically require animals to be head-fixed. Here, we present a method in mice for binge-like ethanol consumpti...
Source: Neuropharmacology - December 6, 2023 Category: Drugs & Pharmacology Authors: Anagha Kalelkar Grayson Sipe Ana Raquel Castro E Costa Ilka M Lorenzo My Nguyen Ivan Linares-Garcia Elena Vazey Rafiq Huda Source Type: research

Chemogenetic inhibition of locus coeruleus to rostromedial tegmental nucleus noradrenergic pathway increases light cycle ethanol drinking in male and female mice and blunts ethanol-induced CTA
Neuropharmacology. 2023 Dec 2:109809. doi: 10.1016/j.neuropharm.2023.109809. Online ahead of print.ABSTRACTWe recently showed that chemogenetic activation of the locus coeruleus (LC) to the rostromedial tegmental nucleus (RMTg) noradrenergic (NE) pathway significantly blunted binge-like ethanol drinking and induced aversive-like behaviors in mice. The aim of the present study is to determine if silencing this TH + LC → RMTg noradrenergic pathway promotes increased levels of binge-like ethanol intake and reduced ethanol-induced conditioned taste aversion (CTA). To this end, both male and female TH-ires-cre mice on a C57BL...
Source: Neuropharmacology - December 4, 2023 Category: Drugs & Pharmacology Authors: Ana Paula S Dornellas Todd E Thiele Montserrat Navarro Source Type: research

Chemogenetic inhibition of locus coeruleus to rostromedial tegmental nucleus noradrenergic pathway increases light cycle ethanol drinking in male and female mice and blunts ethanol-induced CTA
Neuropharmacology. 2023 Dec 2:109809. doi: 10.1016/j.neuropharm.2023.109809. Online ahead of print.ABSTRACTWe recently showed that chemogenetic activation of the locus coeruleus (LC) to the rostromedial tegmental nucleus (RMTg) noradrenergic (NE) pathway significantly blunted binge-like ethanol drinking and induced aversive-like behaviors in mice. The aim of the present study is to determine if silencing this TH + LC → RMTg noradrenergic pathway promotes increased levels of binge-like ethanol intake and reduced ethanol-induced conditioned taste aversion (CTA). To this end, both male and female TH-ires-cre mice on a C57BL...
Source: Neuropharmacology - December 4, 2023 Category: Drugs & Pharmacology Authors: Ana Paula S Dornellas Todd E Thiele Montserrat Navarro Source Type: research

Network-based analysis on the genes and their interactions reveals link between schizophrenia and Alzheimer's disease
In this study, we obtained 331 SCZ-related genes, 650 AD-related genes, 65 shared genes between SCZ and AD. Enrichment analysis shown that these 65 shared genes were mainly involved in cognition, neural development, synaptic transmission, drug reactions, metabolic processes and immune related processes, suggesting a complex mechanism for the co-existence of SCZ and AD. In addition, we performed pathway enrichment analysis and found a total of 57 common pathways between SCZ and AD, which could be largely grouped into three modules: immune module, neurodevelopment module and cancer module. We eventually identified the potent...
Source: Neuropharmacology - December 3, 2023 Category: Drugs & Pharmacology Authors: Pan Guo Chao Meng Shuyue Zhang Yingzi Cai Junkai Huang Jianbo Shu Ju Wang Chunquan Cai Source Type: research

Network-based analysis on the genes and their interactions reveals link between schizophrenia and Alzheimer's disease
In this study, we obtained 331 SCZ-related genes, 650 AD-related genes, 65 shared genes between SCZ and AD. Enrichment analysis shown that these 65 shared genes were mainly involved in cognition, neural development, synaptic transmission, drug reactions, metabolic processes and immune related processes, suggesting a complex mechanism for the co-existence of SCZ and AD. In addition, we performed pathway enrichment analysis and found a total of 57 common pathways between SCZ and AD, which could be largely grouped into three modules: immune module, neurodevelopment module and cancer module. We eventually identified the potent...
Source: Neuropharmacology - December 3, 2023 Category: Drugs & Pharmacology Authors: Pan Guo Chao Meng Shuyue Zhang Yingzi Cai Junkai Huang Jianbo Shu Ju Wang Chunquan Cai Source Type: research

Serotonergic agonism and pharmacologically-induced adolescent stress cause operant-based learning deficits in mice
CONCLUSION: Whilst we revealed dissociable detrimental effects of adolescent CORT treatment and acute 5-HT1A/1B receptor agonism on discrimination and reversal learning, respectively, we did not find evidence of additive deleterious effects of these two treatments. We therefore suggest that while disrupted serotonergic signalling is likely to be involved in the cognitive phenotype of OC-spectrum disorders, distinct neuropathological pathways may be at play in mediating the role of stress as an antecedent in OCD and related illnesses.PMID:38040286 | DOI:10.1016/j.neuropharm.2023.109801 (Source: Neuropharmacology)
Source: Neuropharmacology - December 1, 2023 Category: Drugs & Pharmacology Authors: Carey Wilson Anthony J Hannan Thibault Renoir Source Type: research

Serotonergic agonism and pharmacologically-induced adolescent stress cause operant-based learning deficits in mice
CONCLUSION: Whilst we revealed dissociable detrimental effects of adolescent CORT treatment and acute 5-HT1A/1B receptor agonism on discrimination and reversal learning, respectively, we did not find evidence of additive deleterious effects of these two treatments. We therefore suggest that while disrupted serotonergic signalling is likely to be involved in the cognitive phenotype of OC-spectrum disorders, distinct neuropathological pathways may be at play in mediating the role of stress as an antecedent in OCD and related illnesses.PMID:38040286 | DOI:10.1016/j.neuropharm.2023.109801 (Source: Neuropharmacology)
Source: Neuropharmacology - December 1, 2023 Category: Drugs & Pharmacology Authors: Carey Wilson Anthony J Hannan Thibault Renoir Source Type: research

Specific inhibition of TET1 in the spinal dorsal horn alleviates inflammatory pain in mice by regulating synaptic plasticity
Neuropharmacology. 2023 Nov 25;244:109799. doi: 10.1016/j.neuropharm.2023.109799. Online ahead of print.ABSTRACTDNA demethylation mediated by ten-eleven translocation 1 (TET1) is a critical epigenetic mechanism in which gene expression is regulated via catalysis of 5-methylcytosine to 5-hydroxymethylcytosine. Previously, we demonstrated that TET1 is associated with the genesis of chronic inflammatory pain. However, how TET1 participates in enhanced nociceptive responses in chronic pain remains poorly understood. Here, we report that conditional knockout of Tet1 in dorsal horn neurons via intrathecal injection of rAAV-hSyn-...
Source: Neuropharmacology - November 26, 2023 Category: Drugs & Pharmacology Authors: Kehui Yang Runa Wei Qiaoqiao Liu Yang Tao Zixuan Wu Li Yang Qi-Hui Wang Hongjun Wang Zhiqiang Pan Source Type: research

Specific inhibition of TET1 in the spinal dorsal horn alleviates inflammatory pain in mice by regulating synaptic plasticity
Neuropharmacology. 2023 Nov 24:109799. doi: 10.1016/j.neuropharm.2023.109799. Online ahead of print.ABSTRACTDNA demethylation mediated by ten-eleven translocation 1 (TET1) is a critical epigenetic mechanism in which gene expression is regulated via catalysis of 5-methylcytosine to 5-hydroxymethylcytosine. Previously, we demonstrated that TET1 is associated with the genesis of chronic inflammatory pain. However, how TET1 participates in enhanced nociceptive responses in chronic pain remains poorly understood. Here, we report that conditional knockout of Tet1 in dorsal horn neurons via intrathecal injection of rAAV-hSyn. -Cr...
Source: Neuropharmacology - November 26, 2023 Category: Drugs & Pharmacology Authors: Kehui Yang Runa Wei Qiaoqiao Liu Yang Tao Zixuan Wu Li Yang Qi-Hui Wang Hongjun Wang Zhiqiang Pan Source Type: research

Specific inhibition of TET1 in the spinal dorsal horn alleviates inflammatory pain in mice by regulating synaptic plasticity
Neuropharmacology. 2023 Nov 24:109799. doi: 10.1016/j.neuropharm.2023.109799. Online ahead of print.ABSTRACTDNA demethylation mediated by ten-eleven translocation 1 (TET1) is a critical epigenetic mechanism in which gene expression is regulated via catalysis of 5-methylcytosine to 5-hydroxymethylcytosine. Previously, we demonstrated that TET1 is associated with the genesis of chronic inflammatory pain. However, how TET1 participates in enhanced nociceptive responses in chronic pain remains poorly understood. Here, we report that conditional knockout of Tet1 in dorsal horn neurons via intrathecal injection of rAAV-hSyn. -Cr...
Source: Neuropharmacology - November 26, 2023 Category: Drugs & Pharmacology Authors: Kehui Yang Runa Wei Qiaoqiao Liu Yang Tao Zixuan Wu Li Yang Qi-Hui Wang Hongjun Wang Zhiqiang Pan Source Type: research

Specific inhibition of TET1 in the spinal dorsal horn alleviates inflammatory pain in mice by regulating synaptic plasticity
Neuropharmacology. 2023 Nov 24:109799. doi: 10.1016/j.neuropharm.2023.109799. Online ahead of print.ABSTRACTDNA demethylation mediated by ten-eleven translocation 1 (TET1) is a critical epigenetic mechanism in which gene expression is regulated via catalysis of 5-methylcytosine to 5-hydroxymethylcytosine. Previously, we demonstrated that TET1 is associated with the genesis of chronic inflammatory pain. However, how TET1 participates in enhanced nociceptive responses in chronic pain remains poorly understood. Here, we report that conditional knockout of Tet1 in dorsal horn neurons via intrathecal injection of rAAV-hSyn. -Cr...
Source: Neuropharmacology - November 26, 2023 Category: Drugs & Pharmacology Authors: Kehui Yang Runa Wei Qiaoqiao Liu Yang Tao Zixuan Wu Li Yang Qi-Hui Wang Hongjun Wang Zhiqiang Pan Source Type: research

Increased brain nucleus accumbens functional connectivity in melancholic depression
CONCLUSION: Melancholic depression exhibited different patterns of functional connectivity of the reward circuit relative to non-melancholic patients. This study highlights the significance of the reward circuit in the neuropathology of melancholic depression.PMID:37995807 | DOI:10.1016/j.neuropharm.2023.109798 (Source: Neuropharmacology)
Source: Neuropharmacology - November 23, 2023 Category: Drugs & Pharmacology Authors: Zhaobin Chen Yangpan Ou Feng Liu Huabing Li Ping Li Guangrong Xie Xilong Cui Wenbin Guo Source Type: research

Increased brain nucleus accumbens functional connectivity in melancholic depression
CONCLUSION: Melancholic depression exhibited different patterns of functional connectivity of the reward circuit relative to non-melancholic patients. This study highlights the significance of the reward circuit in the neuropathology of melancholic depression.PMID:37995807 | DOI:10.1016/j.neuropharm.2023.109798 (Source: Neuropharmacology)
Source: Neuropharmacology - November 23, 2023 Category: Drugs & Pharmacology Authors: Zhaobin Chen Yangpan Ou Feng Liu Huabing Li Ping Li Guangrong Xie Xilong Cui Wenbin Guo Source Type: research