Silencing SP1 Alleviated Sevoflurane-Induced POCD Development via Cholinergic Anti-inflammatory Pathway.
Abstract Postoperative cognitive dysfunction (POCD) is a common complication induced by anesthesia or surgery, which affects the concentration, cognition and memory of patients. Sevoflurane, a clinical anesthetic, could stimulate neuro-inflammation and lead to POCD. Recent studies found that specificity protein 1 (SP1) participates in the development of neurological diseases. Our study aims to elucidate the role of SP1 in sevoflurane-induced POCD pathogenesis. We anesthetized Sprague-Dawley rats and treated the primary hippocampal neurons with sevoflurane to construct the in vivo and in vitro POCD models. Besides,...
Source: Neurochemical Research - June 27, 2020 Category: Neuroscience Authors: Lv G, Li C, Wang W, Li N, Wang K Tags: Neurochem Res Source Type: research

Studies on the Neuromodulatory Effects of Ginkgo biloba on Alterations in Lipid Composition and Membrane Integrity of Rat Brain Following Aluminium Neurotoxicity.
Abstract Brain contains the highest lipid content involved in various structural and physiological activities such as structural development, neurogenesis, synaptogenesis, signal transduction and myelin sheath formation. Lipids bilayer is essential to maintain the structural integrity for the physiological functions of protein. Impairments in lipid metabolism and its composition can lead to the progression of various brain ailments such as neurodegenerative and neuropsychiatric disorders. Aluminium (Al), the potent neurotoxin has been linked to Alzheimer's disease (AD) like pathology. Al can bind to biomembrane an...
Source: Neurochemical Research - June 27, 2020 Category: Neuroscience Authors: Verma S, Ranawat P, Nehru B Tags: Neurochem Res Source Type: research

LncRNA MALAT1 Promotes OGD-Induced Apoptosis of Brain Microvascular Endothelial Cells by Sponging miR-126 to Repress PI3K/Akt Signaling Pathway.
This study aimed to investigate the role of MALAT1 in IS in vitro model and the related molecular mechanisms. The expressions of MALAT1 and miR-126 were detected by qPCR. The in vitro IS model was established by treating BMECs with oxygen-glucose deprivation (OGD). Cell viability and cell apoptosis were assessed by MTT assay and flow cytometry, respectively. Luciferase assay was conducted to examine the interplay between MALAT1 and miR-126. Western blotting was used to determine the protein levels of apoptosis-associated proteins (e.g. caspase 3, Bax and Bcl-2) and PI3K/Akt pathway-related proteins (e.g. PI3K, Akt, p-PI3K,...
Source: Neurochemical Research - June 26, 2020 Category: Neuroscience Authors: Zhang L, Yang H, Li WJ, Liu YH Tags: Neurochem Res Source Type: research

Retraction Note to: MicroRNA-322 Cluster Promotes Tau Phosphorylation via Targeting Brain-Derived Neurotrophic Factor.
Abstract The Editors have retracted this article [1] following an investigation conducted by the journal. After publication concerns were raised regarding interpretation of the data presented in Fig. 4. The Editors requested additional data and clarification to confirm interpretation of data results. After further review, the Editors found that the additional data were not adequate to support the conclusion of the article and that P-values for the additional data were based on improper statistical analyses. With more appropriate statistical analysis, the reported effects for miR-322 and BDNF were not statistically...
Source: Neurochemical Research - June 25, 2020 Category: Neuroscience Authors: Zhang J, Liu Z, Pei Y, Yang W, Xie C, Long S Tags: Neurochem Res Source Type: research

Brain Microvascular Endothelial Cell Derived Exosomes Potently Ameliorate Cognitive Dysfunction by Enhancing the Clearance of A β Through Up-Regulation of P-gp in Mouse Model of AD.
Brain Microvascular Endothelial Cell Derived Exosomes Potently Ameliorate Cognitive Dysfunction by Enhancing the Clearance of Aβ Through Up-Regulation of P-gp in Mouse Model of AD. Neurochem Res. 2020 Jun 24;: Authors: Pan J, He R, Huo Q, Shi Y, Zhao L Abstract Accumulation of amyloid-β (Aβ) peptides in the brain is regarded as a major contributor to the pathogenesis and progression of Alzheimer's disease (AD). P-glycoprotein (P-gp) as a member of ABC transporter family situated in blood brain barrier (BBB) plays a role on cleaning of Aβ via its efflux transport effect in the treat...
Source: Neurochemical Research - June 24, 2020 Category: Neuroscience Authors: Pan J, He R, Huo Q, Shi Y, Zhao L Tags: Neurochem Res Source Type: research

A Novel Compound YS-5-23 Exhibits Neuroprotective Effect by Reducing β-Site Amyloid Precursor Protein Cleaving Enzyme 1's Expression and H2O2-Induced Cytotoxicity in SH-SY5Y Cells.
A Novel Compound YS-5-23 Exhibits Neuroprotective Effect by Reducing β-Site Amyloid Precursor Protein Cleaving Enzyme 1's Expression and H2O2-Induced Cytotoxicity in SH-SY5Y Cells. Neurochem Res. 2020 Jun 18;: Authors: Cheng C, Zheng N, Sun D, Fang W, Zheng L, Song W, Huang J Abstract The abnormally accumulated amyloid-β (Aβ) and oxidative stress contribute to the initiation and progression of Alzheimer's disease (AD). β-site amyloid precursor protein cleaving enzyme 1 (BACE1) is the rate-limiting enzyme for the production of Aβ. Furthermore, Aβ was reported to increase o...
Source: Neurochemical Research - June 18, 2020 Category: Neuroscience Authors: Cheng C, Zheng N, Sun D, Fang W, Zheng L, Song W, Huang J Tags: Neurochem Res Source Type: research

Polyphenols from Toona sinensiss Seeds Alleviate Neuroinflammation Induced by 6-Hydroxydopamine Through Suppressing p38 MAPK Signaling Pathway in a Rat Model of Parkinson's Disease.
Abstract Polyphenols from Toona sinensis seeds (PTSS) have demonstrated anti-inflammatory effects in various diseases, while the anti-neuroinflammatory effects still remain to be investigated. We aimed to investigate the effects of PTSS on Parkinson's disease and underlying mechanisms using a rat model. We employed 6-hydroxydopamine (6-OHDA) to male Sprague Dawley (SD) rats and PC12 cells to construct the in vivo and vitro models of PD and dopaminergic (DA) neuron injury, respectively. Cell viability was detected by cell counting kit-8 (CCK-8) assay and protein levels of inflammatory mediators and some p38 MAPK pa...
Source: Neurochemical Research - June 17, 2020 Category: Neuroscience Authors: Zhuang W, Cai M, Li W, Chen C, Wang Y, Lv E, Fu W Tags: Neurochem Res Source Type: research

Sodium Butyrate Exacerbates Parkinson's Disease by Aggravating Neuroinflammation and Colonic Inflammation in MPTP-Induced Mice Model.
This study sought to evaluate how butyrate, one of SCFAs, affect the pathology in a subacute 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine hydrochloride (MPTP) treated mouse model of PD. Sodium butyrate (NaB; 165 mg/kg/day i.g., 7 days) was administrated from the day after the last MPTP injection. Interestingly, NaB significantly aggravated MPTP-induced motor dysfunction (P 
Source: Neurochemical Research - June 16, 2020 Category: Neuroscience Authors: Qiao CM, Sun MF, Jia XB, Li Y, Zhang BP, Zhao LP, Shi Y, Zhou ZL, Zhu YL, Cui C, Shen YQ Tags: Neurochem Res Source Type: research

Transplantation of Mesenchymal Stromal Cells Expressing the Human Preproenkephalin Gene Can Relieve Pain in a Rat Model of Neuropathic Pain.
In conclusion, our study shows that the TAS improves BMSC transgenic therapy for neuropathic pain treatment. PMID: 32529390 [PubMed - as supplied by publisher] (Source: Neurochemical Research)
Source: Neurochemical Research - June 11, 2020 Category: Neuroscience Authors: Yang J, Zhang L, Xie P, Pan M, Ma G Tags: Neurochem Res Source Type: research

Cerebellar Predominant Increase in mRNA Expression Levels of Sirt1 and Sirt3 Isoforms in a Transgenic Mouse Model of Huntington's Disease.
i L, Klivényi P, Zádori D Abstract The potential role of Sirt1 and Sirt2 subtypes of Sirtuins (class III NAD+-dependent deacetylases) in the pathogenesis of Huntington's disease (HD) has been extensively studied yielding some controversial results. However, data regarding the involvement of Sirt3 and their variants in HD are considerably limited. The aim of this study was to assess the expression pattern of Sirt1 and three Sirt3 mRNA isoforms (Sirt3-M1/2/3) in the striatum, cortex and cerebellum in respect of the effect of gender, age and the presence of the transgene using the N171-82Q transgenic mo...
Source: Neurochemical Research - June 10, 2020 Category: Neuroscience Authors: Salamon A, Maszlag-Török R, Veres G, Boros FA, Vágvölgyi-Sümegi E, Somogyi A, Vécsei L, Klivényi P, Zádori D Tags: Neurochem Res Source Type: research

Antidepressant Effect and Modulation of the Redox System Mediated by Tannic Acid on Lipopolysaccharide-Induced Depressive and Inflammatory Changes in Mice.
In conclusion, TA shows many neuroprotective properties, with demonstrated antioxidant, anti-inflammatory and antidepressant effects in this animal model of acute depressive-like behavior. Therefore, this compound could provide an alternative therapeutic approach for the treatment of depression. PMID: 32500408 [PubMed - as supplied by publisher] (Source: Neurochemical Research)
Source: Neurochemical Research - June 5, 2020 Category: Neuroscience Authors: Luduvico KP, Spohr L, Soares MSP, Teixeira FC, de Farias AS, Bona NP, Pedra NS, de Oliveira Campello Felix A, Spanevello RM, Stefanello FM Tags: Neurochem Res Source Type: research

A Critical Role for ISGylation, Ubiquitination and, SUMOylation in Brain Damage: Implications for Neuroprotection.
Abstract Post-translational modification (PTMs) of proteins by ubiquitin and ubiquitin-like modifiers such as interferon-stimulated gene 15 (ISG15) and small ubiquitin-related modifier (SUMO) play a critical role in the regulation of brain pathophysiology. Protein ISGylation is a covalent attachment of ISG15 to its target proteins, which is a unique PTM among other ubiquitin-like modifiers. Although, ISG15 shares sequence homology to ubiquitin, yet the functional significance of protein ISGylation is distinct from ubiquitination and SUMOylation. Further, ISG15 highly conserved among vertebrate species, unlike the ...
Source: Neurochemical Research - June 4, 2020 Category: Neuroscience Authors: Nakka VP, Mohammed AQ Tags: Neurochem Res Source Type: research

Chronic Manganese Administration with Longer Intervals Between Injections Produced Neurotoxicity and Hepatotoxicity in Rats.
Abstract Subacute exposure to manganese (Mn) produced Parkinson's disease-like syndrome called Manganism. Chronic onset and progression are characteristics of Manganism, therefore, this study aimed to examine Mn toxicity following chronic exposures. Male Sprague-Dawley rats were injected Mn2+ 1 and 5 mg/kg, every 10 days for 150 days (15 injections). Animal body weight and behavioral activities were recorded. At the end of experiments, the brain and liver were collected for morphological and molecular analysis. Chronic Mn exposure did not affect animal body weight gain, but the high dose of Mn treat...
Source: Neurochemical Research - June 2, 2020 Category: Neuroscience Authors: Fan XM, Luo Y, Cao YM, Xiong TW, Song S, Liu J, Fan QY Tags: Neurochem Res Source Type: research

Neuroprotective Role of Quercetin on Rotenone-Induced Toxicity in SH-SY5Y Cell Line Through Modulation of Apoptotic and Autophagic Pathways.
Abstract The detrimental impact on the food chain due to the overuse of rotenone is partly responsible for alpha-synuclein (α-syn) mediated neurotoxicity. It is hypothesized that rotenone overdose leads to cytosolic proteopathy resulting in modulation of apoptosis and autophagic pathways. The aim of our study is to explore the neuroprotective role of quercetin, a beneficial polyphenol against rotenone-induced neurotoxicity in dopaminergic human SH-SY5Y cell lines. In our study we demonstrated the correlation of rotenone-induced neurotoxicity through elevation of intracellular reactive oxygen species (ROS) an...
Source: Neurochemical Research - June 1, 2020 Category: Neuroscience Authors: Pakrashi S, Chakraborty J, Bandyopadhyay J Tags: Neurochem Res Source Type: research

Mulberry Fruit Extract Alleviates Cognitive Impairment by Promoting the Clearance of Amyloid- β and Inhibiting Neuroinflammation in Alzheimer's Disease Mice.
Mulberry Fruit Extract Alleviates Cognitive Impairment by Promoting the Clearance of Amyloid-β and Inhibiting Neuroinflammation in Alzheimer's Disease Mice. Neurochem Res. 2020 Jun 01;: Authors: Liu D, Du D Abstract Alzheimer's disease (AD) is a common neurodegenerative disease, always clinically manifesting with memory loss and other cognitive abilities serious enough to interfere with daily life. Over the past decade, the extensive accumulation of Aβ plaques and the related neuroinflammation such as the activation of glial cells have been frequently demonstrated to contribute to the pathog...
Source: Neurochemical Research - June 1, 2020 Category: Neuroscience Authors: Liu D, Du D Tags: Neurochem Res Source Type: research

MCC950 Inhibits NLRP3 Inflammasome and Alleviates Axonal Injures in Early Stages of Diffuse Axonal Injury in Rats.
Abstract Increasing evidence has revealed that neuroinflammation plays a pivotal role in axonal injures. Nucleotide oligomerization domain (NOD)-like receptor protein (NLRP3) inflammasome is reported to be widely involved with the pathology of central nervous system disorders. But the role of NLRP3 in diffuse axonal injury (DAI) are rarely reported. The purpose of this study was to investigate the expression of NLRP3 after diffuse axonal injury and the role of NLRP3 in axonal injures. The lateral head rotation device was used to establish DAI model of rats. Immunohistochemical staining for β-amyloid precursor...
Source: Neurochemical Research - May 30, 2020 Category: Neuroscience Authors: Zhao J, Guo X, Wang B, Yang Z, Huang T, Guo D, Zhang M, Song J Tags: Neurochem Res Source Type: research

Promising Polyphenols in Parkinson's Disease Therapeutics.
Abstract Parkinson's disease (PD) is a slow progressive, second most common neurodegenerative disease characterized by the loss of dopaminergic neurons from the nigrostriatal pathway. In spite of extensive research the therapeutics options of disease are limited which only offer symptomatic relief and could not prevent the disease progression. Therefore researchers are looking for the probable synthetic or natural compounds for the PD therapeutics. Due to mandatory chronic consumption of anti PD drug to the PD patients, the natural compounds are getting attention recently. Numerous studies have indicated the neuro...
Source: Neurochemical Research - May 27, 2020 Category: Neuroscience Authors: Singh A, Tripathi P, Yadawa AK, Singh S Tags: Neurochem Res Source Type: research

The Involvement of Mitochondrial Biogenesis in Selenium Reduced Hyperglycemia-Aggravated Cerebral Ischemia Injury.
Abstract Selenium has been shown to possess antioxidant and neuroprotective effects by modulating mitochondrial function and activating mitochondrial biogenesis. Our previous study has also suggested that selenium protected neurons against glutamate toxicity and hyperglycemia-induced damage by regulating mitochondrial fission and fusion. However, it is still not known whether the mitochondrial biogenesis is involved in selenium alleviating hyperglycemia-aggravated cerebral ischemia reperfusion (I/R) injury. The object of this study is to define whether selenium protects neurons against hyperglycemia-aggravated cer...
Source: Neurochemical Research - May 23, 2020 Category: Neuroscience Authors: Yang L, Ma YM, Shen XL, Fan YC, Zhang JZ, Li PA, Jing L Tags: Neurochem Res Source Type: research

β-Caryophyllene Liposomes Attenuate Neurovascular Unit Damage After Subarachnoid Hemorrhage in Rats.
This study was conducted to prepare β-caryophyllene loaded liposomes (BCP-LP) and investigated their effects on neurovascular unit (NVU) damage after subarachnoid hemorrhage (SAH) in rats. A blood injection into the pre-chiasmatic cistern was used to achieve SAH. BCP-LP were prepared, characterized and administrated to rats with SAH. The prepared BCP-LP were spherical with a size distribution of approximately 189.3 nm and Zeta potential of - 13.9 mV. Neurological scoring, the balance beam test, cerebral blood flow monitoring, brain edema and biochemical analyses were applied to evaluate the effects of B...
Source: Neurochemical Research - May 22, 2020 Category: Neuroscience Authors: Wang F, Teng Z, Liu D, Wang Y, Lou J, Dong Z Tags: Neurochem Res Source Type: research

Neuroprotective Effects of Lacosamide and Memantine on Hyperoxic Brain Injury in Rats.
This study aimed to evaluate neuroprotective effects of lacosamide and memantine treatment in a hyperoxia-induced brain injury model in immature rats. This study was performed in the Animal Experiments Laboratory of Dokuz Eylul University Faculty of Medicine. Neonatal Wistar strain rat pups were exposed to hyperoxia (80% oxygen + 20% nitrogen) for five days postnatally. They were divided into five groups; hyperoxia + lacosamide, hyperoxia + memantine, hyperoxia + lacosamide and memantine, hyperoxia + saline, control groups. After termination of the exper...
Source: Neurochemical Research - May 22, 2020 Category: Neuroscience Authors: Polat İ, Cilaker Mıcılı S, Çalışır M, Bayram E, Yiş U, Ayanoğlu M, Okur D, Edem P, Paketçi C, Tuğyan K, Yılmaz O, Hız Kurul S Tags: Neurochem Res Source Type: research

Antidepressant-Like Activities of Hispidol and Decursin in Mice and Analysis of Neurotransmitter Monoamines.
Abstract The antidepressant activities of hispidol and decursin (both potent monoamine oxidase A (MAO-A) inhibitors) were evaluated using the forced swimming test (FST) and the tail suspension test (TST) in mice, and thereafter, levels of neurotransmitter monoamines and metabolites in brain tissues were analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Hispidol (15 mg/kg) caused less or comparable immobility than fluoxetine (15 mg/kg; the positive control) in immobility time, as determined by FST (9.6 vs 32.0 s) and TST (53.1 vs 48.7 s), respectively, and its effects were dose-dependent and si...
Source: Neurochemical Research - May 21, 2020 Category: Neuroscience Authors: Oh JM, Lee HS, Baek SC, Lee JP, Jeong GS, Paik MJ, Kim H Tags: Neurochem Res Source Type: research

L-Aspartate, L-Ornithine and L-Ornithine-L-Aspartate (LOLA) and Their Impact on Brain Energy Metabolism.
CD Abstract L-Ornithine-L-aspartate (LOLA), a crystalline salt, is used primarily in the management of hepatic encephalopathy. The degree to which it might penetrate the brain, and the effects it might have on metabolism in brain are poorly understood. Here, to investigate the effects of LOLA on brain energy metabolism we incubated brain cortical tissue slices from guinea pig (Cavea porcellus) with the constituent amino acids of LOLA, L-ornithine or L-aspartate, as well as LOLA, in the presence of [1-13C]D-glucose and [1,2-13C]acetate; these labelled substrates are useful indicators of brain metabolic activity. L...
Source: Neurochemical Research - May 18, 2020 Category: Neuroscience Authors: Das A, Fröhlich D, Achanta LB, Rowlands BD, Housley GD, Klugmann M, Rae CD Tags: Neurochem Res Source Type: research

HAP1 Modulates Epileptic Seizures by Regulating GABAAR Function in Patients with Temporal Lobe Epilepsy and in the PTZ-Induced Epileptic Model.
This study provides evidence that hippocampal HAP1 is linked to GABAARs in evoking seizures and suggests that this mechanism is involved in epileptic seizures in the brain, representing a potential therapeutic target for epilepsy. PMID: 32419121 [PubMed - as supplied by publisher] (Source: Neurochemical Research)
Source: Neurochemical Research - May 17, 2020 Category: Neuroscience Authors: Li R, Wu B, He M, Zhang P, Zhang Q, Deng J, Yuan J, Chen Y Tags: Neurochem Res Source Type: research

Special Issue in Honour of Michael B Robinson.
PMID: 32418081 [PubMed - as supplied by publisher] (Source: Neurochemical Research)
Source: Neurochemical Research - May 16, 2020 Category: Neuroscience Authors: Beart PM Tags: Neurochem Res Source Type: research

Capsaicin and Its Analogues Impede Nocifensive Response of Caenorhabditis elegans to Noxious Heat.
Abstract Capsaicin is the most abundant pungent molecule identified in red chili peppers, and it is widely used for food flavoring, in pepper spray for self-defense devices and recently in ointments for the relief of neuropathic pain. Capsaicin and several other related vanilloid compounds are secondary plant metabolites. Capsaicin is a selective agonist of the transient receptor potential channel, vanilloid subfamily member 1 (TRPV1). After exposition to vanilloid solution, Caenorhabditis elegans wild type (N2) and mutants were placed on petri dishes divided in quadrants for heat stimulation. Thermal avoidance in...
Source: Neurochemical Research - May 16, 2020 Category: Neuroscience Authors: Nkambeu B, Salem JB, Beaudry F Tags: Neurochem Res Source Type: research

Matrix Metalloproteinase-9 Overexpression Regulates Hippocampal Synaptic Plasticity and Decreases Alcohol Consumption and Preference in Mice.
Abstract Brain matrix metalloproteinases (MMPs) have been recently implicated in alcohol addiction; however, the molecular mechanisms remain poorly understood. Matrix metalloproteinase-9 (MMP-9), an extrasynaptic protease, is the best described MMP that is thought to regulate addictive behavior. In the present study, the effect of MMP-9 overexpression on hippocampal neuron plasticity and alcoholic behavior was assessed in spontaneous alcohol drinking mice. Two-bottle choice model showed that the overexpression of MMP-9 in the hippocampus developed by adeno-associated virus (AAV) could decrease alcohol consumption ...
Source: Neurochemical Research - May 15, 2020 Category: Neuroscience Authors: Yin LT, Xie XY, Xue LY, Yang XR, Jia J, Zhang Y, Zhang C Tags: Neurochem Res Source Type: research

Early Intervention of Gastrodin Improved Motor Learning in Diabetic Rats Through Ameliorating Vascular Dysfunction.
Abstract The mechanism of cognitive dysfunction in diabetes is still unclear. Recently, studies have shown that the cerebellum is involved in cognition. Furthermore, diabetes-induced cerebellar alterations is related to vascular changes. Therefore, we aimed to explore the roles of vascular function in diabetes-induced cerebellar damage and motor learning deficits. Type 1 diabetes was induced by a single injection of streptozotocin in Sprague-Dawley rats. Motor learning was assessed by beam walk test and beam balance test. The pathological changes of the cerebellum were assessed by Hematoxylin and eosin staining an...
Source: Neurochemical Research - May 15, 2020 Category: Neuroscience Authors: Zhang F, Deng CK, Huang YJ, Miao YH, Wang YY, Zhang Y, Qian ZY, Zhang WQ, Zhou RD, Lei B, Shen X, Wu XY, Cui G, Song JL, Mu ZH, Zou YY Tags: Neurochem Res Source Type: research

Safflower Yellow Improves the Synaptic Structural Plasticity by Ameliorating the Disorder of Glutamate Circulation in A β1-42-induced AD Model Rats.
In this study, we aimed to clarify whether the effects of SY and HSYA on the learning and memory abilities of Aβ1-42-induced AD model rats are related to the enhancement of synaptic structural plasticity in brain tissues and the amelioration of disorder of glutamate circulation. We used rats injected with Aβ1-42 into the bilateral hippocampus as a model of AD. After treatment with SY and HSYA, the learning and memory abilities of the Aβ1-42-induced AD model rats were enhanced, Aβ deposition in the AD model rats was decreased, structural damage to dendritic spines and the loss of synaptic-associated prot...
Source: Neurochemical Research - May 14, 2020 Category: Neuroscience Authors: Hou J, Wang C, Zhang M, Ren M, Yang G, Qu Z, Hu Y Tags: Neurochem Res Source Type: research

Mutant TDP-43 Expression Triggers TDP-43 Pathology and Cell Autonomous Effects on Primary Astrocytes: Implications for Non-cell Autonomous Pathology in ALS.
Abstract Motor neuron degeneration in amyotrophic lateral sclerosis (ALS) caused by mutations in superoxide dismutase 1 (SOD1) is partly non-cell autonomous, involving cellular dysfunction of astrocytes. Whether non-cell autonomous effects occur in other forms of ALS, such as TAR DNA binding protein 43 (TDP-43)-related disease, remains unclear. Here, we characterised the impact of mutant TDP-43 expression on primary astrocytes derived from transgenic TDP-43A315T mice. Mutant TDP-43 astrocytes revealed evidence for TDP-43 pathology, shown by cytoplasmic TDP-43 inclusions and accumulation in insoluble cell fractions...
Source: Neurochemical Research - May 14, 2020 Category: Neuroscience Authors: Barton SK, Lau CL, Chiam MDF, Tomas D, Muyderman H, Beart PM, Turner BJ Tags: Neurochem Res Source Type: research

A Novel Mechanism of Specialized Proresolving Lipid Mediators Mitigating Radicular Pain: The Negative Interaction with NLRP3 Inflammasome.
Abstract Inhibition of immune and inflammatory reaction induced by the expose of nucleus pulposus (NP) could effectively ameliorate neuropathic pain in the lumbar disc herniation. Maresin1 (MaR1), as a macrophage-derived mediator of inflammation resolution, displayed potent anti-inflammatory action. In the present study, we attempted to elucidate the impact of MaR1 on radicular pain and the interaction with NLRP3 inflammasome. We established a rat model of non-compressive lumbar disc herniation and different administration (MaR1 or Caspase-1 inhibitor) was given to them. The paw withdrawal latency (PWL) and paw wi...
Source: Neurochemical Research - May 14, 2020 Category: Neuroscience Authors: Wang YH, Li Y, Wang JN, Zhao QX, Wen S, Wang SC, Sun T Tags: Neurochem Res Source Type: research

Effects of Diclofenac Versus Meloxicam in Pentylenetetrazol-Kindled Mice.
Abstract Epilepsy comes after stroke as the most common chronic neurological disorder worldwide. Inflammation enhances neuronal hyperexcitability that could provide a background setting for the development of epilepsy. The aim of this study was to assess the effect of valproate (VAL), diclofenac (DIC), meloxicam (MEL), VAL + MEL and VAL + DIC in pentylenetetrazol (PTZ) kindled mice. Seventy mice were randomly allocated into 7 equal groups; Control, PTZ, VAL, DIC, MEL, VAL + MEL and VAL + DIC groups. Kindling was induced by PTZ (40 mg/kg, i.p.) injection ...
Source: Neurochemical Research - May 13, 2020 Category: Neuroscience Authors: Elgarhi R, Shehata MM, Abdelsameea AA, Salem AE Tags: Neurochem Res Source Type: research

Olfactory Dysfunction in Diabetic Rats is Associated with miR-146a Overexpression and Inflammation.
lo E, Guevara-Guzmán R Abstract Type 2 diabetes (T2D) is associated with cognitive decline and dementia. Both neurodegenerative conditions are characterized by olfactory dysfunction (OD) which is also observed in diabetic patients. Diabetes and neurodegeneration display altered miRNAs expression; therefore, the study of miRNAs in the diabetic olfactory system is important in order to know the mechanisms involved in neurodegeneration induced by T2D. In this work we evaluated the expression of miRs206, 451, 146a and 34a in the olfactory bulb (OB) of T2D rats and its association with OD. T2D induction was perf...
Source: Neurochemical Research - May 13, 2020 Category: Neuroscience Authors: Jiménez A, Organista-Juárez D, Torres-Castro A, Guzmán-Ruíz MA, Estudillo E, Guevara-Guzmán R Tags: Neurochem Res Source Type: research

The Reducible Disulfide Proteome of Synaptosomes Supports a Role for Reversible Oxidations of Protein Thiols in the Maintenance of Neuronal Redox Homeostasis.
Abstract The mechanisms by which neurons maintain redox homeostasis, disruption of which is linked to disease, are not well known. Hydrogen peroxide, a major cellular oxidant and neuromodulator, can promote reversible oxidations of protein thiols but the scope, targets, and significance of such oxidations occurring in neurons, especially in vivo, are uncertain. Using redox phenylarsine oxide (PAO)-affinity chromatography, which exploits the high-affinity of trivalent arsenicals for protein dithiols, this study investigated the occurrence of reducible and, therefore, potentially regulatory, protein disulfide bonds ...
Source: Neurochemical Research - May 12, 2020 Category: Neuroscience Authors: Foley TD, Montovano G, Camacho Ayala M Tags: Neurochem Res Source Type: research

MiR-210-3p Inhibits Proliferation and Migration of C6 Cells by Targeting Iscu.
Abstract Glioma is the most common primary brain tumor and the most malignant type of glioma is glioblastoma with the character of high mortality, high recurrence rate and poor prognosis. MicroRNAs act as an important component in glioma development and thus may be a potential target for the treatment of glioma. There were some researches indicated that miR-210-3p played a role in glioma development, but if it can inhibit glioma growth, as well as the underlying mechanism, is still uncertain. In the present study, we investigated the effects of miR-210-3p and its potential target gene Iscu on glioma (C6) cells pro...
Source: Neurochemical Research - May 9, 2020 Category: Neuroscience Authors: He H, Liu J, Li W, Yao X, Ren Q, Shen B, Xue C, Zou L, Zhao H, Qin J, Jin G Tags: Neurochem Res Source Type: research

Anxiolytic and Anti-depressive Like Effects of Translocator Protein (18  kDa) Ligand YL-IPA08 in a Rat Model of Postpartum Depression.
In this study, the classical method of reproductive hormone withdrawal was used to construct a rat model of postpartum depression (PPD). The effect of YL-IPA08, a new ligand compound of TSPO, on PPD was evaluated using multiple behavioral tests at progressive time points. Additionally, real-time quantitative PCR, Western-blotting and an enzyme linked immunosorbent assay were conducted to elucidate the potential molecular mechanism of such effect. We report that the levels of TSPO and neurosteroids in the hippocampus and prefrontal cortex were significantly decreased in PPD rats compared to healthy controls. After 3 we...
Source: Neurochemical Research - May 7, 2020 Category: Neuroscience Authors: Ren P, Ma L, Wang JY, Guo H, Sun L, Gao ML, Liu YZ, Ma YQ, Li YF, Guo WZ Tags: Neurochem Res Source Type: research

Sevoflurane Induces Hippocampal Neuronal Apoptosis by Altering the Level of Neuropeptide Y in Neonatal Rats.
In this study, postnatal day 7 (PND7) Sprague-Dawley rats and primary cultured cells separated from hippocampi were exposed to sevoflurane (2.4% for 4 h) and the NPY expression levels after treatment were analyzed. Furthermore, neuronal apoptosis assay was conducted via immunofluorescence staining of cleaved caspase-3 and flow cytometry after exogenous NPY administration to PND7 rats as well as cultured hippocampal neurons to elucidate the role of NPY in sevoflurane-induced neurotoxicity. Our results showed the level of NPY gradually decreased within 24 h after sevoflurane exposure in both the hippocampus of PND7...
Source: Neurochemical Research - May 6, 2020 Category: Neuroscience Authors: Kang W, Lu D, Yang X, Ma W, Chen X, Chen K, Xu X, Zhou X, Zhou L, Feng X Tags: Neurochem Res Source Type: research

Combination Therapy of Gabapentin and N-Acetylcysteine Against Posttraumatic Epilepsy in Rats.
Abstract Traumatic brain injury (TBI) is a major public health problem worldwide that is associated with increased mortality and morbidity. Posttraumatic epilepsy (PTE) is one of the sequelae of TBI. The aim of this study was to investigate the role of N-acetylcysteine (NAC) as an adjuvant on the efficacy of levetiracetam (LEV) and gabapentin (GBP) in PTE model encouraged by pentylenetetrazol (PTZ) after mild-TBI in male Sprague-Dawley rats. Mild-TBI was performed by the weight-drop method in male Sprague-Dawley rats. PTE model was developed by injecting PTZ (30+15+15 mg/kg, 30 min intervals, i.p.) 7&nbs...
Source: Neurochemical Research - May 5, 2020 Category: Neuroscience Authors: Efendioglu M, Basaran R, Akca M, Ceman D, Demirtas C, Yildirim M Tags: Neurochem Res Source Type: research

Inactivation of the Ventral Pallidum by GABAA Receptor Agonist Promotes Non-rapid Eye Movement Sleep in Rats.
Abstract GABA, the most abundant inhibitory neurotransmitter in the brain, is closely linked with sleep and wakefulness. As the largest area input to the ventral pallidum (VP), the nucleus accumbens (NAc) has been confirmed to play a pivotal role in promoting non-rapid eye movement (NREM) sleep through inhibitory projections from NAc adenosine A2A receptor-expressing neurons to VP GABAergic neurons which mostly express GABAA receptors. Although these studies demonstrate the possible role of VP GABAergic neurons in sleep-wake regulation, whether and how its modulate sleep-wake cycle is not completely clear. In...
Source: Neurochemical Research - May 4, 2020 Category: Neuroscience Authors: Zhang X, Liu Y, Yang B, Xu H Tags: Neurochem Res Source Type: research

Extracellular Vesicles Isolated from Human Induced Pluripotent Stem Cell-Derived Neurons Contain a Transcriptional Network.
Abstract Healthy brain function is mediated by several complementary signalling pathways, many of which are driven by extracellular vesicles (EVs). EVs are heterogeneous in both size and cargo and are constitutively released from cells into the extracellular milieu. They are subsequently trafficked to recipient cells, whereupon their entry can modify the cellular phenotype. Here, in order to further analyse the mRNA and protein cargo of neuronal EVs, we isolated EVs by size exclusion chromatography from human induced pluripotent stem cell (iPSC)-derived neurons. Electron microscopy and dynamic light scattering rev...
Source: Neurochemical Research - May 2, 2020 Category: Neuroscience Authors: Hicks DA, Jones AC, Corbett NJ, Fisher K, Pickering-Brown SM, Ashe MP, Hooper NM Tags: Neurochem Res Source Type: research

LncRNA H19 Attenuates Apoptosis in MPTP-Induced Parkinson's Disease Through Regulating miR-585-3p/PIK3R3.
Abstract Parkinson's disease (PD) is a prevalent age-related neurodegenerative disease which is modulated by various molecules, including long non-coding RNAs (lncRNAs). LncRNA H19 has been shown to be associated with PD progression, but the mechanism is still unclear. This research aims to investigate the role of H19 in PD development and the detailed mechanisms. Our results showed that H19 was down-regulated in brain tissue of MPTP-induced PD mice (in vivo) and in MPP+ treated human neuroblastoma cells. miR-585-3p was verified to be a target of lncRNA H19 and was negatively regulated by H19. In addition, H19 cou...
Source: Neurochemical Research - April 30, 2020 Category: Neuroscience Authors: Zhang Y, Xia Q, Lin J Tags: Neurochem Res Source Type: research

Long Non-coding RNA MINCR Regulates miR-876-5p/GSPT1 Axis to Aggravate Glioma Progression.
Abstract Emerging evidence underlined the crucial roles played by long non-coding RNAs (lncRNAs) in glioma. MINCR has been reported in multiple malignancies. Here, we studied its function and potential mechanism in glioma, which remain unclear. Gene expressions were analyzed by qRT-PCR assay. Both in vitro and in vivo assays were conducted to evaluate the cellular function of MINCR in glioma. The subcellular situation of MINCR was detected by subcellular fractionation and FISH assays. Luciferase reporter, RNA pull-down and RNA immunoprecipitation (RIP) assays were combined to investigate potential mechanisms of re...
Source: Neurochemical Research - April 24, 2020 Category: Neuroscience Authors: Li Z, Xie X, Fan X, Li X Tags: Neurochem Res Source Type: research

Hypercapnia Exacerbates the Blood-Brain Barrier Disruption Via Promoting HIF-1a Nuclear Translocation in the Astrocytes of the Hippocampus: Implication in Further Cognitive Impairment in Hypoxemic Adult Rats.
Abstract Hypercapnia in combination with hypoxemia is usually present in severe respiratory disease in the intensive care unit (ICU) and can lead to more severe cognitive dysfunction. Increasing evidence has indicated that the compromised blood-brain barrier (BBB) in the hippocampus in hypoxemia conditions can result in cognitive dysfunction. However, the role and underlying mechanism of hypercapnia in the BBB disruption remains poorly known. A rat model of hypercapnia was first established in this study by intubation and mechanical ventilation with a small-animal ventilator. After this, the cognitive function of ...
Source: Neurochemical Research - April 23, 2020 Category: Neuroscience Authors: Liu X, Ding H, Li X, Deng Y, Liu X, Wang K, Wen M, Chen S, Jiang W, Zeng H Tags: Neurochem Res Source Type: research

Correction to: Intrathecal Epigallocatechin Gallate Treatment Improves Functional Recovery After Spinal Cord Injury by Upregulating the Expression of BDNF and GDNF.
Abstract Since the publication of our article [1] it has come to our attention that there was an error in Figure 4 in which the bottom left immunochemistry panel Control/Bax was a duplication of the bottom right immunohistochemistry panel EGCG/GDNF in Figure 3. PMID: 32323106 [PubMed - as supplied by publisher] (Source: Neurochemical Research)
Source: Neurochemical Research - April 22, 2020 Category: Neuroscience Authors: Tian W, Han XG, Liu YJ, Tang GQ, Liu B, Wang YQ, Xiao B, Xu YF Tags: Neurochem Res Source Type: research

Role of Insulin Resistance in the Alzheimer's Disease Progression.
Abstract Recent studies continue to find evidence linking Type 2 diabetes (T2D) with Alzheimer's disease (AD), the most common cause of dementia, a general term for memory loss and other cognitive abilities serious enough to interfere with daily life. Insulin resistance or dysfunction of insulin signaling is a universal feature of T2D, the main culprit for altered glucose metabolism and its interdependence on cell death pathways, forming the basis of linking T2D with AD as it may exacerbate Aβ accumulation, tau hyperphosphorylation and devastates glucose transportation, energy metabolism, hippocampal framewor...
Source: Neurochemical Research - April 20, 2020 Category: Neuroscience Authors: Nguyen TT, Ta QTH, Nguyen TTD, Le TT, Vo VG Tags: Neurochem Res Source Type: research

The Effect of Organophosphate Exposure on Neuronal Cell Coenzyme Q10 Status.
Abstract Organophosphate (OP) compounds are widely used as pesticides and herbicides and exposure to these compounds has been associated with both chronic and acute forms of neurological dysfunction including cognitive impairment, neurophysiological problems and cerebral ataxia with evidence of mitochondrial impairment being associated with this toxicity. In view of the potential mitochondrial impairment, the present study aimed to investigate the effect of exposure to commonly used OPs, dichlorvos, methyl-parathion (parathion) and chloropyrifos (CPF) on the cellular level of the mitochondrial electron transport c...
Source: Neurochemical Research - April 18, 2020 Category: Neuroscience Authors: Turton N, Heaton RA, Ismail F, Roberts S, Nelder S, Phillips S, Hargreaves IP Tags: Neurochem Res Source Type: research

High Estrogen Level Modifies Postoperative Hyperalgesia via GPR30 and MMP-9 in Dorsal Root Ganglia Neurons.
Abstract The cycling of sex hormones is one of the factors affecting pain in females, and the mechanisms are not fully understood. G-protein coupled estrogen receptor 30 (GPR30) is the estrogen receptor known to be involved in mechanical hyperalgesia. Studies have demonstrated that matrix metalloproteinase-9 (MMP-9) is a critical component in peripheral/central nervous system hypersensitivity and neuroinflammation, both of which participate in hyperalgesia. Here, ovariectomized rats were treated with low or high dose estrogen replacement, and then plantar incisions were made. Subsequently, mechanical allodynia was...
Source: Neurochemical Research - April 17, 2020 Category: Neuroscience Authors: Jiang M, Liu Y, Wu H, Ma Z, Gu X Tags: Neurochem Res Source Type: research

The Downregulation of Truncated TrkB Receptors Modulated by MicroRNA-185 Activates Full-Length TrkB Signaling and Suppresses the Epileptiform Discharges in Cultured Hippocampal Neurons.
In this study, to regulate the expression of truncated TrkB receptor and full-length TrkB signaling, rno-miR-185-3p was transduced into the SREDs model. Then, the changes in the activity of L-type voltage-gated calcium channels (VGCCs) and in epileptiform discharges were investigated. Transduction of rno-miR-185-3p downregulated the expression of truncated TrkB and dramatically activated full-length TrkB signaling in the model. Next, we found that the activation of full-length TrkB signaling decreased the maximal Ca2+ current density in the model, delayed the steady-state activation and accelerated the inactivation of L-ty...
Source: Neurochemical Research - April 16, 2020 Category: Neuroscience Authors: Xie W, Xiang L, Song Y, Tian X Tags: Neurochem Res Source Type: research

Effects of Delphinidin on Pathophysiological Signs of Nucleus Basalis of Meynert Lesioned Rats as Animal Model of Alzheimer Disease.
Abstract Alzheimer's disease (AD) is an advanced neurodegenerative disorder greatly accompanied by cognitive deficits, oxidative stress, inflammation, amyloid plaques deposition, and acetylcholinesterase (AChE) hyper-activation. Growing evidence suggests natural compounds with antioxidant and anti-inflammatory features improve pathophysiological signs of AD. The present study was designed to investigate the effects of Delphinidin (25, 50 mg/kg) as an anthocyanidin on spatial memory impairment and AD hallmarks such as hippocampal AChE activity, amyloid plaques deposition, oxidative stress and expression of amy...
Source: Neurochemical Research - April 15, 2020 Category: Neuroscience Authors: Heysieattalab S, Sadeghi L Tags: Neurochem Res Source Type: research

Glutathione in Brain: Overview of Its Conformations, Functions, Biochemical Characteristics, Quantitation and Potential Therapeutic Role in Brain Disorders.
Abstract Glutathione (GSH) is an important antioxidant found abundantly and synthesized intracellularly in the cytosol in a tightly regulated fashion. It has diverse physiological functions, including protection against reactive oxygen species and nitrogen species, antioxidant defense as well as maintenance of cellular thiol status. The human brain due to the high oxygen consumption is extremely susceptible to the generation of reactive oxygen species. GSH plays a paramount role in brain antioxidant defense, maintaining redox homeostasis. The depletion of brain GSH has also been observed from both autopsies as wel...
Source: Neurochemical Research - April 15, 2020 Category: Neuroscience Authors: Dwivedi D, Megha K, Mishra R, Mandal PK Tags: Neurochem Res Source Type: research

The Thiol-Modifier Effects of Organoselenium Compounds and Their Cytoprotective Actions in Neuronal Cells.
This study was aimed to investigate and compare the protective effects of two well known [(PhSe)2 and PhSeZnCl] and two newly developed (MRK Picolyl and MRK Ester) organoselenium compounds against oxidative challenge in cultured neuronal HT22 cells. The thiol peroxidase and oxidase activities were performed using the glutathione reductase (GR)-coupled assay. In order to evaluate protective effects of the organoselenium compounds against oxidative challenge in neuronal HT22 cells, experiments based on glutamate-induced oxytosis and SIN-1-mediated peroxynitrite generation were performed. The thiol peroxidase activities of th...
Source: Neurochemical Research - April 13, 2020 Category: Neuroscience Authors: Galant LS, Rafique J, Braga AL, Braga FC, Saba S, Radi R, da Rocha JBT, Santi C, Monsalve M, Farina M, de Bem AF Tags: Neurochem Res Source Type: research