ABBV-744 induces autophagy in gastric cancer cells by regulating PI3K/AKT/mTOR/p70S6k and MAPK signaling pathways
In this study, we evaluated the effect of ABBV-744 on gastric cancer cells and explored the possible underlying mechanisms. We found that ABBV-744 inhibited the growth of gastric cancer cells and patient-derived tumor organoids in a dose-dependent manner. Cellular experiments revealed that ABBV-744 induced mitochondria damage, reactive oxygen species accumulation, cell cycle arrest and apoptotic cell death in gastric cancer cells. Transcriptomic analysis using RNA-sequencing data identified autophagy as a crucial pathway involved in the cell death caused by ABBV-744. Mechanically, further studies showed that ABBV-744 induc...
Source: Neoplasia - September 28, 2023 Category: Cancer & Oncology Authors: Kun Wang Jiatong Tang Shengxian Fan Haochen Su Ranran Yu Yixuan Zhang Hao Wu Ying Lv Shu Zhang Xiaoping Zou Source Type: research
HSP90 C-terminal domain inhibition promotes VDAC1 oligomerization via decreasing K274 mono-ubiquitination in Hepatocellular Carcinoma
In this study, we focused on the effect of the C-terminal domain of Hsp90 on VDAC1 oligomerization, ubiquitination, and VDAC1 channel activity. We found that Hsp90 C-terminal domain inhibitor Novobiocin promoted VDAC1 oligomerization, release of cytochrome c, and activated mitochondrial apoptosis pathway. Atomic coarse particle modeling simulation revealed C-terminal domain of Hsp90α stabilized VDAC1 monomers. The purified VDAC1 was reconstituted into a planar lipid bilayer, and electrophysiology experiments of patch clamp showed that the Hsp90 C-terminal inhibitor Novobiocin increased VDAC1 channel conductance via promot...
Source: Neoplasia - September 17, 2023 Category: Cancer & Oncology Authors: Jinxin Zhang Lixia Liu Yan Li Yaling Huang Senbo Xiao Zihao Deng Zhenming Zheng Jieyou Li Manfeng Liang Guantai Xie Xiao Chen Yaotang Deng Wenchong Tan Hairou Su Guibing Wu Chunqing Cai Xuemei Chen Fei Zou Source Type: research
HSP90 C-terminal domain inhibition promotes VDAC1 oligomerization via decreasing K274 mono-ubiquitination in Hepatocellular Carcinoma
In this study, we focused on the effect of the C-terminal domain of Hsp90 on VDAC1 oligomerization, ubiquitination, and VDAC1 channel activity. We found that Hsp90 C-terminal domain inhibitor Novobiocin promoted VDAC1 oligomerization, release of cytochrome c, and activated mitochondrial apoptosis pathway. Atomic coarse particle modeling simulation revealed C-terminal domain of Hsp90α stabilized VDAC1 monomers. The purified VDAC1 was reconstituted into a planar lipid bilayer, and electrophysiology experiments of patch clamp showed that the Hsp90 C-terminal inhibitor Novobiocin increased VDAC1 channel conductance via promot...
Source: Neoplasia - September 17, 2023 Category: Cancer & Oncology Authors: Jinxin Zhang Lixia Liu Yan Li Yaling Huang Senbo Xiao Zihao Deng Zhenming Zheng Jieyou Li Manfeng Liang Guantai Xie Xiao Chen Yaotang Deng Wenchong Tan Hairou Su Guibing Wu Chunqing Cai Xuemei Chen Fei Zou Source Type: research
HSP90 C-terminal domain inhibition promotes VDAC1 oligomerization via decreasing K274 mono-ubiquitination in Hepatocellular Carcinoma
In this study, we focused on the effect of the C-terminal domain of Hsp90 on VDAC1 oligomerization, ubiquitination, and VDAC1 channel activity. We found that Hsp90 C-terminal domain inhibitor Novobiocin promoted VDAC1 oligomerization, release of cytochrome c, and activated mitochondrial apoptosis pathway. Atomic coarse particle modeling simulation revealed C-terminal domain of Hsp90α stabilized VDAC1 monomers. The purified VDAC1 was reconstituted into a planar lipid bilayer, and electrophysiology experiments of patch clamp showed that the Hsp90 C-terminal inhibitor Novobiocin increased VDAC1 channel conductance via promot...
Source: Neoplasia - September 17, 2023 Category: Cancer & Oncology Authors: Jinxin Zhang Lixia Liu Yan Li Yaling Huang Senbo Xiao Zihao Deng Zhenming Zheng Jieyou Li Manfeng Liang Guantai Xie Xiao Chen Yaotang Deng Wenchong Tan Hairou Su Guibing Wu Chunqing Cai Xuemei Chen Fei Zou Source Type: research
HSP90 C-terminal domain inhibition promotes VDAC1 oligomerization via decreasing K274 mono-ubiquitination in Hepatocellular Carcinoma
In this study, we focused on the effect of the C-terminal domain of Hsp90 on VDAC1 oligomerization, ubiquitination, and VDAC1 channel activity. We found that Hsp90 C-terminal domain inhibitor Novobiocin promoted VDAC1 oligomerization, release of cytochrome c, and activated mitochondrial apoptosis pathway. Atomic coarse particle modeling simulation revealed C-terminal domain of Hsp90α stabilized VDAC1 monomers. The purified VDAC1 was reconstituted into a planar lipid bilayer, and electrophysiology experiments of patch clamp showed that the Hsp90 C-terminal inhibitor Novobiocin increased VDAC1 channel conductance via promot...
Source: Neoplasia - September 17, 2023 Category: Cancer & Oncology Authors: Jinxin Zhang Lixia Liu Yan Li Yaling Huang Senbo Xiao Zihao Deng Zhenming Zheng Jieyou Li Manfeng Liang Guantai Xie Xiao Chen Yaotang Deng Wenchong Tan Hairou Su Guibing Wu Chunqing Cai Xuemei Chen Fei Zou Source Type: research
HSP90 C-terminal domain inhibition promotes VDAC1 oligomerization via decreasing K274 mono-ubiquitination in Hepatocellular Carcinoma
In this study, we focused on the effect of the C-terminal domain of Hsp90 on VDAC1 oligomerization, ubiquitination, and VDAC1 channel activity. We found that Hsp90 C-terminal domain inhibitor Novobiocin promoted VDAC1 oligomerization, release of cytochrome c, and activated mitochondrial apoptosis pathway. Atomic coarse particle modeling simulation revealed C-terminal domain of Hsp90α stabilized VDAC1 monomers. The purified VDAC1 was reconstituted into a planar lipid bilayer, and electrophysiology experiments of patch clamp showed that the Hsp90 C-terminal inhibitor Novobiocin increased VDAC1 channel conductance via promot...
Source: Neoplasia - September 17, 2023 Category: Cancer & Oncology Authors: Jinxin Zhang Lixia Liu Yan Li Yaling Huang Senbo Xiao Zihao Deng Zhenming Zheng Jieyou Li Manfeng Liang Guantai Xie Xiao Chen Yaotang Deng Wenchong Tan Hairou Su Guibing Wu Chunqing Cai Xuemei Chen Fei Zou Source Type: research
HSP90 C-terminal domain inhibition promotes VDAC1 oligomerization via decreasing K274 mono-ubiquitination in Hepatocellular Carcinoma
In this study, we focused on the effect of the C-terminal domain of Hsp90 on VDAC1 oligomerization, ubiquitination, and VDAC1 channel activity. We found that Hsp90 C-terminal domain inhibitor Novobiocin promoted VDAC1 oligomerization, release of cytochrome c, and activated mitochondrial apoptosis pathway. Atomic coarse particle modeling simulation revealed C-terminal domain of Hsp90α stabilized VDAC1 monomers. The purified VDAC1 was reconstituted into a planar lipid bilayer, and electrophysiology experiments of patch clamp showed that the Hsp90 C-terminal inhibitor Novobiocin increased VDAC1 channel conductance via promot...
Source: Neoplasia - September 17, 2023 Category: Cancer & Oncology Authors: Jinxin Zhang Lixia Liu Yan Li Yaling Huang Senbo Xiao Zihao Deng Zhenming Zheng Jieyou Li Manfeng Liang Guantai Xie Xiao Chen Yaotang Deng Wenchong Tan Hairou Su Guibing Wu Chunqing Cai Xuemei Chen Fei Zou Source Type: research
HSP90 C-terminal domain inhibition promotes VDAC1 oligomerization via decreasing K274 mono-ubiquitination in Hepatocellular Carcinoma
In this study, we focused on the effect of the C-terminal domain of Hsp90 on VDAC1 oligomerization, ubiquitination, and VDAC1 channel activity. We found that Hsp90 C-terminal domain inhibitor Novobiocin promoted VDAC1 oligomerization, release of cytochrome c, and activated mitochondrial apoptosis pathway. Atomic coarse particle modeling simulation revealed C-terminal domain of Hsp90α stabilized VDAC1 monomers. The purified VDAC1 was reconstituted into a planar lipid bilayer, and electrophysiology experiments of patch clamp showed that the Hsp90 C-terminal inhibitor Novobiocin increased VDAC1 channel conductance via promot...
Source: Neoplasia - September 17, 2023 Category: Cancer & Oncology Authors: Jinxin Zhang Lixia Liu Yan Li Yaling Huang Senbo Xiao Zihao Deng Zhenming Zheng Jieyou Li Manfeng Liang Guantai Xie Xiao Chen Yaotang Deng Wenchong Tan Hairou Su Guibing Wu Chunqing Cai Xuemei Chen Fei Zou Source Type: research
HSP90 C-terminal domain inhibition promotes VDAC1 oligomerization via decreasing K274 mono-ubiquitination in Hepatocellular Carcinoma
In this study, we focused on the effect of the C-terminal domain of Hsp90 on VDAC1 oligomerization, ubiquitination, and VDAC1 channel activity. We found that Hsp90 C-terminal domain inhibitor Novobiocin promoted VDAC1 oligomerization, release of cytochrome c, and activated mitochondrial apoptosis pathway. Atomic coarse particle modeling simulation revealed C-terminal domain of Hsp90α stabilized VDAC1 monomers. The purified VDAC1 was reconstituted into a planar lipid bilayer, and electrophysiology experiments of patch clamp showed that the Hsp90 C-terminal inhibitor Novobiocin increased VDAC1 channel conductance via promot...
Source: Neoplasia - September 17, 2023 Category: Cancer & Oncology Authors: Jinxin Zhang Lixia Liu Yan Li Yaling Huang Senbo Xiao Zihao Deng Zhenming Zheng Jieyou Li Manfeng Liang Guantai Xie Xiao Chen Yaotang Deng Wenchong Tan Hairou Su Guibing Wu Chunqing Cai Xuemei Chen Fei Zou Source Type: research
HSP90 C-terminal domain inhibition promotes VDAC1 oligomerization via decreasing K274 mono-ubiquitination in Hepatocellular Carcinoma
In this study, we focused on the effect of the C-terminal domain of Hsp90 on VDAC1 oligomerization, ubiquitination, and VDAC1 channel activity. We found that Hsp90 C-terminal domain inhibitor Novobiocin promoted VDAC1 oligomerization, release of cytochrome c, and activated mitochondrial apoptosis pathway. Atomic coarse particle modeling simulation revealed C-terminal domain of Hsp90α stabilized VDAC1 monomers. The purified VDAC1 was reconstituted into a planar lipid bilayer, and electrophysiology experiments of patch clamp showed that the Hsp90 C-terminal inhibitor Novobiocin increased VDAC1 channel conductance via promot...
Source: Neoplasia - September 17, 2023 Category: Cancer & Oncology Authors: Jinxin Zhang Lixia Liu Yan Li Yaling Huang Senbo Xiao Zihao Deng Zhenming Zheng Jieyou Li Manfeng Liang Guantai Xie Xiao Chen Yaotang Deng Wenchong Tan Hairou Su Guibing Wu Chunqing Cai Xuemei Chen Fei Zou Source Type: research
Increased expression of IDO1 is associated with improved survival and increased number of TILs in patients with high-grade serous ovarian cancer
CONCLUSIONS: Our study provides evidence that IDO1 expression serves as a positive prognostic marker in HGSOC and is associated with an increased number of CD3+, CD4+ and CD8+ TILs. Understanding the intricate relationship between IDO1, TILs, and the tumor microenvironment may hold the key to improving outcomes in HGSOC.PMID:37703626 | PMC:PMC10502412 | DOI:10.1016/j.neo.2023.100934 (Source: Neoplasia)
Source: Neoplasia - September 13, 2023 Category: Cancer & Oncology Authors: Inga Hoffmann Mihnea P Dragomir Nanna Monj é Carlotta Keunecke Catarina Alisa Kunze Simon Schallenberg Sofya Marchenko Wolfgang D Schmitt Hagen Kulbe Jalid Sehouli Ioana Elena Braicu Paul Jank Carsten Denkert Silvia Darb-Esfahani David Horst Bruno V Sinn Source Type: research
Increased expression of IDO1 is associated with improved survival and increased number of TILs in patients with high-grade serous ovarian cancer
CONCLUSIONS: Our study provides evidence that IDO1 expression serves as a positive prognostic marker in HGSOC and is associated with an increased number of CD3+, CD4+ and CD8+ TILs. Understanding the intricate relationship between IDO1, TILs, and the tumor microenvironment may hold the key to improving outcomes in HGSOC.PMID:37703626 | PMC:PMC10502412 | DOI:10.1016/j.neo.2023.100934 (Source: Neoplasia)
Source: Neoplasia - September 13, 2023 Category: Cancer & Oncology Authors: Inga Hoffmann Mihnea P Dragomir Nanna Monj é Carlotta Keunecke Catarina Alisa Kunze Simon Schallenberg Sofya Marchenko Wolfgang D Schmitt Hagen Kulbe Jalid Sehouli Ioana Elena Braicu Paul Jank Carsten Denkert Silvia Darb-Esfahani David Horst Bruno V Sinn Source Type: research
Increased expression of IDO1 is associated with improved survival and increased number of TILs in patients with high-grade serous ovarian cancer
CONCLUSIONS: Our study provides evidence that IDO1 expression serves as a positive prognostic marker in HGSOC and is associated with an increased number of CD3+, CD4+ and CD8+ TILs. Understanding the intricate relationship between IDO1, TILs, and the tumor microenvironment may hold the key to improving outcomes in HGSOC.PMID:37703626 | DOI:10.1016/j.neo.2023.100934 (Source: Neoplasia)
Source: Neoplasia - September 13, 2023 Category: Cancer & Oncology Authors: Inga Hoffmann Mihnea P Dragomir Nanna Monj é Carlotta Keunecke Catarina Alisa Kunze Simon Schallenberg Sofya Marchenko Wolfgang D Schmitt Hagen Kulbe Jalid Sehouli Ioana Elena Braicu Paul Jank Carsten Denkert Silvia Darb-Esfahani David Horst Bruno V Sinn Source Type: research
Increased expression of IDO1 is associated with improved survival and increased number of TILs in patients with high-grade serous ovarian cancer
CONCLUSIONS: Our study provides evidence that IDO1 expression serves as a positive prognostic marker in HGSOC and is associated with an increased number of CD3+, CD4+ and CD8+ TILs. Understanding the intricate relationship between IDO1, TILs, and the tumor microenvironment may hold the key to improving outcomes in HGSOC.PMID:37703626 | DOI:10.1016/j.neo.2023.100934 (Source: Neoplasia)
Source: Neoplasia - September 13, 2023 Category: Cancer & Oncology Authors: Inga Hoffmann Mihnea P Dragomir Nanna Monj é Carlotta Keunecke Catarina Alisa Kunze Simon Schallenberg Sofya Marchenko Wolfgang D Schmitt Hagen Kulbe Jalid Sehouli Ioana Elena Braicu Paul Jank Carsten Denkert Silvia Darb-Esfahani David Horst Bruno V Sinn Source Type: research
Mutational signatures and their association with survival and gene expression in urological carcinomas
Neoplasia. 2023 Sep 5;44:100933. doi: 10.1016/j.neo.2023.100933. Online ahead of print.ABSTRACTDifferent sources of mutagenesis cause consistently identifiable patterns of mutations and mutational signatures that mirror the various carcinogenetic processes. We used publicly available data from the Cancer Genome Atlas to evaluate the associations between the activity of the mutational signatures and various survival endpoints in six types of urological cancers after adjusting for established prognostic factors. The predictive power of the signatures was evaluated with dynamic area under curve models. In addition, links betw...
Source: Neoplasia - September 7, 2023 Category: Cancer & Oncology Authors: Peeter Karihtala Outi Kilpivaara Katja Porvari Source Type: research
