γH2AX prefers late replicating metaphase chromosome regions
Publication date: Available online 4 June 2018Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Ana Laura Reyes-Ábalos, Pablo Liddle, Gustavo A. Folle, María Vittoria Di TomasoAbstractDNA damage response (DDR) constitutes a protein pathway to handle eukaryotic DNA lesions in the context of chromatin. DDR engages the recruitment of signaling, transducer, effector, chromatin modifiers and remodeling proteins, allowing cell cycle delay, DNA repair or induction of senescence or apoptosis. An early DDR-event includes the epigenetic phosphorylation of the histone variant H2AX on s...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - July 6, 2018 Category: Genetics & Stem Cells Source Type: research

GENETIC DAMAGE IN ENVIRONMENTALLY EXPOSED POPULATIONS TO OPEN-PIT COAL MINING RESIDUES: ANALYSIS OF BUCCAL MICRONUCLEUS CYTOME (BMN-cyt) ASSAY AND ALKALINE, ENDO III AND FPG HIGH-THROUGHPUT COMET ASSAY
This study aimed to evaluate DNA damage using the cytome assay (BMN-cyt) in buccal cells and its relation to primary and oxidative DNA damage in lymphocytes, assessed by the high-throughput alkaline and modified (FPG-ENDO III) Comet assay in individuals with environmental exposure to coal mining residues in northern Colombia. Considering metals from coal mining activities as the main source of reactive oxygen species (ROS) generation, the concentrations of inorganic elements in blood samples was also assessed. The analysis revealed that frequencies of BMN-cyt parameters related to DNA damage (micronuclei), cytokinesis (bin...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - July 6, 2018 Category: Genetics & Stem Cells Source Type: research

Increased genetic damage found in waste picker women in a landfill in Paraguay measured by comet assay and the micronucleus test
Publication date: Available online 7 June 2018Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Deidamia Franco de Diana, Jaime Segovia Abreu, Diana Castiglioni Serafini, José Francisco Ortíz, María José Samaniego, Ana Carina Aranda, Enrique Zamorano-PonceAbstractCateura landfill located in Asunción-Paraguay is the site of final disposal of tons of garbage of all kinds coming from urban, industrial and commercial areas. Due to an inadequate waste management system, there is a big concern in our country on how it can affect people working within the landf...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - July 6, 2018 Category: Genetics & Stem Cells Source Type: research

Single nucleotide polymorphisms in ATM, TNF-α and IL6 genes and risk of radiotoxicity in breast cancer patients
Publication date: Available online 7 June 2018Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Elisa E. Córdoba, Ezequiel Lacunza, Martín C. Abba, Eduardo Fernández, Alba M. GüerciAbstractAlthough oncological therapies have improved in the last decades, breast cancer (BC) remains a serious health problem worldwide. Radiotherapy (RT) is one of the most frequently used treatments for cancer aimed at eliminating tumor cells. However, it can also alter the surrounding normal tissue, especially the skin, and patient reactions may vary as a result of extrinsic and int...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - July 6, 2018 Category: Genetics & Stem Cells Source Type: research

Evaluatıon of health effects, genetıc damage and telomere length ın children exposed to arsenic in West Bengal, İndia
Publication date: Available online 7 June 2018Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Debmita Chatterjee, Shanta Adak, Nilanjana Banerjee, Pritha Bhattacharjee, Apurba K. Bandyopadhyay, Ashok K. GiriAbstractIncreasing evidence of arsenic contamination in ground water and its associated adverse health outcomes affects millions of people worldwide. However, arsenic toxicity studies in children have gained impetus very recently due to the non-prominence of the hallmarks of arsenic toxicity i.e skin lesions. We recognized the need to evaluate the status of genetic damage brought abo...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - July 6, 2018 Category: Genetics & Stem Cells Source Type: research

Evaluation of resveratrol radiomodifying potential for radiotherapy treatment
Publication date: Available online 8 June 2018Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Yuliana C. Banegas, Eliana E. Ocolotobiche, Gisel Padula, Elisa E. Córdoba, Eduardo Fernández, Alba M. GüerciAbstractRadiotherapy is widely used for cancer treatment. However, its adverse effects that may develop during the course of treatment have forced to search agents to protect biological systems against the deleterious effects of radiation. Resveratrol (3,4,5-trihydroxy-trans-stilbene; RSV) is a natural polyphenol currently promoted for its beneficial pleiotropic effect...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - July 6, 2018 Category: Genetics & Stem Cells Source Type: research

Assessment of genomic instability and proliferation index in cultured lymphocytes of patients with Down syndrome, congenital anomalies and aplastic anaemia
Publication date: Available online 8 June 2018Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Alex George, Shriram Venkatesan, Narmada Ashok, Radha Saraswathy, M Prakash HandeAbstractOne hundred and fifteen cases [Down Syndrome (DS) n = 75, Multiple Congenital Anomalies (MCA) n = 15 and Aplastic Anaemia (AA) n = 25], with respect to their nature of predisposition to cancer, were selected for clinical, cytogenetic and cyto-molecular studies to understand the severity of genomic instability according to the nature of the different diseases. Cytogenetic studies included chromos...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - July 6, 2018 Category: Genetics & Stem Cells Source Type: research

Modulation of chromatin conformation by the histone deacetylase inhibitor trichostatin A promotes the removal of radiation-induced lesions in ataxia telangiectasia cell lines
Publication date: Available online 15 June 2018Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Alessandra Egidi, Silvia Filippi, Federico Manganello, Wilner Lopez-Martinez, Roberta MeschiniAbstractAtaxia telangiectasia is a rare autosomal recessive genome instability syndrome caused by mutations in the Ataxia Telangiectasia Mutated gene and characterized by a very high sensitivity to agents inducing double strand breaks such as ionizing radiation. In cells derived from ataxia telangiectasia patients a prominent enhancement of chromosomal aberrations is revealed as a consequence of this ...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - July 6, 2018 Category: Genetics & Stem Cells Source Type: research

Telomere shortening associated with increased levels of oxidative stress in sulfur mustard-exposed Iranian veterans
In this study, lymphocytic telomere lengths and serum levels of isoprostane F2α were measured using q-PCR and enzyme immunoassay-based methods in 40 Iranian veterans who had been exposed to SM between 1983-88 and 40 non-exposed healthy volunteers. The relative telomere length in SM-exposed individuals was found to be significantly shorter than the non-exposed individuals. In addition, the level of 8-isoprostane F2α was significantly higher in the SM-exposed group compared to controls. Oxidative stress can be caused by defective antioxidant responses following gene mutations or altered activities of antioxidant ...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - July 6, 2018 Category: Genetics & Stem Cells Source Type: research

Protective effects of neocuproine copper chelator against oxidative damage in NSC34 cells
Publication date: Available online 25 June 2018Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Emilene A. Nunes, Tânia M. Manieri, Andreza C. Matias, Fernanda R. Bertuchi, Daniela A. da Silva, Larissa Lago, Roseli H. Sato, Giselle CerchiaroAbstractIn this work, we aim to provide evidence for the protective effect of a copper chelator, neocuproine (NeoCu), against the oxidative stress in NSC34 cells, which inhibits biomolecule oxidation and cell death. Results obtained with the comet assay allowed to determine the increase in oxidized purines and pyrimidines by H2O2 exposure, and t...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - July 6, 2018 Category: Genetics & Stem Cells Source Type: research

Titanium dioxide nanoparticles: Toxicity and genotoxicity in Drosophila melanogaster (SMART eye-spot test and comet assay in neuroblasts)
Publication date: July 2018Source: Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Volume 831Author(s): Sara Sario, Amélia M. Silva, Isabel GaivãoAbstractTitanium dioxide nanoparticles (TiO2NP) are used in the food, drug, and cosmetics industries and evaluation of their human and environmental toxicity is required. We have tested the toxicity of TiO2NP (anatase) with respect to developmental effects and DNA damage in Drosophila melanogaster strain Ok, using the eye-spot Somatic Mutation and Recombination Test (SMART) and the comet assay (neuroblasts). For the survival assay, TiO2NP were su...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - July 6, 2018 Category: Genetics & Stem Cells Source Type: research

A statistical approach for analyzing data from the in vivo Pig-a gene mutation assay
Publication date: July 2018Source: Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Volume 831Author(s): Bernd-Wolfgang Igl, Stephen D. Dertinger, Vasily N. Dobrovolsky, Marian Raschke, Andreas Sutter, Richardus VonkAbstractThe in vivo Pig-a gene mutation assay serves to evaluate the genotoxic potential of chemicals. In the rat blood-based assay, the lack of CD59 on the surface of erythrocytes is quantified via fluorophore-labeled antibodies in conjunction with flow cytometric analysis to determine the frequency of Pig-a mutant phenotype cells. The assay has achieved regulatory relevance as it is suggest...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - July 6, 2018 Category: Genetics & Stem Cells Source Type: research

Editorial Board
Publication date: July 2018Source: Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Volume 831Author(s): (Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis)
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - July 6, 2018 Category: Genetics & Stem Cells Source Type: research

Metformin prolongs survival rate in mice and causes increased excretion of cell-free DNA in the urine of X-irradiated rats
Publication date: July 2018Source: Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Volume 831Author(s): Serazhutdin Abdullaev, Gulchachak Minkabirova, Ekaterina Karmanova, Vadim Bruskov, Azhub GazievAbstractAn antidiabetic drug metformin has anticarcinogenic and geroprotective effects and has been used in combination with radiation cancer therapy. The present work is devoted to the study of the effect of metformin on survival in mice, the frequency of micronuclei in mouse bone marrow cells and excretion of cell-free nuclear and mitochondrial DNA in the urine of X-ray-exposed rats. The survival rate and ...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - July 6, 2018 Category: Genetics & Stem Cells Source Type: research

Discovering human germ cell mutagens with whole genome sequencing: Insights from power calculations reveal the importance of controlling for between-family variability
Publication date: July 2018Source: Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Volume 831Author(s): R.J. Webster, A. Williams, F. Marchetti, C.L. YaukAbstractMutations in germ cells pose potential genetic risks to offspring. However, de novo mutations are rare events that are spread across the genome and are difficult to detect. Thus, studies in this area have generally been under-powered, and no human germ cell mutagen has been identified. Whole Genome Sequencing (WGS) of human pedigrees has been proposed as an approach to overcome these technical and statistical challenges. WGS enables analysis of...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - July 6, 2018 Category: Genetics & Stem Cells Source Type: research

GSTP1 c.341C>T gene polymorphism increases the risk of oral squamous cell carcinoma
Publication date: July 2018Source: Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Volume 831Author(s): Deepa Rajesh, Sharath Balakrishna, S.M. Azeem Mohiyuddin, T.N. Suresh, A.V. Moideen KuttyAbstractGlutathione S Transferases (GST) are anti-oxidant enzymes involved in detoxification of cellular and exogenous carcinogens and oxidative products of reactive oxygen species. Genetic polymorphisms can attenuate the detoxification capacity of GST and consequently increase the susceptibility to carcinogenesis. There are eight classes of GST enzymes of which pi subtype is the predominant form expressed in the ...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - July 6, 2018 Category: Genetics & Stem Cells Source Type: research

Early antioxidant responses via the concerted activation of NF-κB and Nrf2 characterize the gamma-radiation-induced adaptive response in quiescent human peripheral blood mononuclear cells
Publication date: July 2018Source: Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Volume 831Author(s): Neha Paraswani, Maikho Thoh, Hari N. Bhilwade, Anu GhoshAbstractThe radiation-induced adaptive response (RI-AR) is a non-targeted effect which is outside the scope of the classical Linear-No-Threshold (LNT) dose-response paradigm. However, the mechanisms of the RI-AR are not well understood. We have studied the RI-AR in quiescent human peripheral blood mononuclear cells (PBMCs). PBMCs in G0 phase were ‘primed’ with a low dose (100 mGy gamma radiation) and then, after an ‘adaptive w...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - July 6, 2018 Category: Genetics & Stem Cells Source Type: research

Multi-laboratory evaluation of 1,3-propane sultone, N-propyl-N-nitrosourea, and mitomycin C in the Pig-a mutation assay in vivo
Publication date: July 2018Source: Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Volume 831Author(s): Young-Shin Chung, Bumsoo Pak, Sehee Han, Jiyeon Lee, Jiyoung Kim, Seng-Min Back, Cho-Rong Park, Su-Hwan Kim, Jong-Kwon LeeAbstractThe mutagenic potencies of 1,3-propane sultone (PS), N-propyl-N-nitrosourea (PNU), and mitomycin C (MMC) were investigated in three independent laboratories in Korea using the Pig-a assay in vivo. Sprague-Dawley rats were treated with vehicle or test substance on three consecutive days. Blood samples were collected for measuring Pig-a mutant phenotypes (CD59-deficient eryth...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - July 6, 2018 Category: Genetics & Stem Cells Source Type: research

Genotoxicity of disinfection byproducts and disinfected waters: A review of recent literature
Publication date: July 2018Source: Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Volume 831Author(s): Constanza Cortés, Ricard MarcosAbstractThe presence of water disinfection byproducts (DBPs) in tap water, resulting from disinfection processes involving chlorination or chloramination, increases the mutagenicity of the water and may pose adverse health effects. The topic was reviewed by DeMarini and coworkers in 2007. Here, we review research on the genotoxicity of DBPs published since that time. Studies, primarily using the Salmonella mutagenicity assay, have continued to show that chlorinati...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - July 6, 2018 Category: Genetics & Stem Cells Source Type: research

Detrimental effects detected in exfoliated buccal cells from anesthesiology medical residents occupationally exposed to inhalation anesthetics: an observational study
Publication date: Available online 3 July 2018Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Mariana G. Braz, Kátina M. Souza, Lorena M.C. Lucio, Giulia C.C. Di Renzo, Luciana M. Feliciano, João Paulo C. Marcondes, C.-Y. Oliver Chen, José Reinaldo C. Braz, Leandro G. BrazAbstractOperating room professionals are scarcely aware of their individual occupational exposure to waste anesthetic gases (WAGs). Medical residents spend several hours per day in operating rooms and consequently experience occupational exposure to WAGs. Considering that no studies have yet evalua...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - July 6, 2018 Category: Genetics & Stem Cells Source Type: research

Expression patterns and mutation analysis of p53 in fish Rita rita from polluted riverine environment
Publication date: August 2018Source: Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Volumes 832–833Author(s): Tandrima Mitra, Bimal Prasanna Mohanty, Sasmita Mohanty, Gopal Krishna Purohit, Basanta Kumar DasAbstractThe present study was undertaken to investigate the alterations in gene expression patterns and for mutation analysis of p53 in the riverine catfish Rita rita collected from polluted riverine habitat. The partial p53 gene sequence of Rita rita generated showed a high degree of similarities with the DNA binding domains of fishes, mice and human. Transcriptomic analysis, carried out by q...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - July 6, 2018 Category: Genetics & Stem Cells Source Type: research

Identifying germ cell mutagens using OECD test guideline 488 (transgenic rodent somatic and germ cell gene mutation assays) and integration with somatic cell testing
Publication date: August 2018Source: Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Volumes 832–833Author(s): Francesco Marchetti, Marilyn J. Aardema, Carol Beevers, Jan van Benthem, Roger Godschalk, Andrew Williams, Carole L. Yauk, Robert Young, George R. DouglasAbstractThe Organisation for Economic Co-operation and Development Test Guideline 488 (TG 488) provides recommendations for assessing germ cell and somatic cell mutagenicity using transgenic rodent (TGR) models. However, important data gaps exist for selecting an optimal approach for simultaneously evaluating mutagenicity in both cell ty...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - July 6, 2018 Category: Genetics & Stem Cells Source Type: research

Simulation of mouse and rat spermatogenesis to inform genotoxicity testing using OECD test guideline 488
Publication date: August 2018Source: Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Volumes 832–833Author(s): Francesco Marchetti, Marilyn Aardema, Carol Beevers, Jan van Benthem, George R. Douglas, Roger Godschalk, Carole L. Yauk, Robert Young, Andrew WilliamsAbstractThe Organisation for Economic Co-operation and Development Test Guideline (TG) 488 for the transgenic rodent (TGR) mutation assay recommends two sampling times for assessing germ cell mutagenicity following the required 28-day exposure period: 28 + > 49 days for mouse sperm and 28 + >70 days for rat sperm from th...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - July 6, 2018 Category: Genetics & Stem Cells Source Type: research

Effects of age and gender on the baseline and 2 Gy 60Co γ-ray-induced nucleoplasmic bridges frequencies in the peripheral blood lymphocytes of Chinese population
Publication date: August 2018Source: Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Volumes 832–833Author(s): Tian-Jing Cai, Xue Lu, Xue-Lei Tian, Hua Zhao, Shuang Li, Jiang-Bin Feng, De-Qing Chen, Mei Tian, Qing-Jie LiuAbstractBaseline nucleoplasmic bridges (NPB) vary widely in the general population from different regions in the same country or from different countries. The baseline NPB level in the normal Chinese population and the factors affecting the baseline and radiation-induced NPB levels have not been explored yet. The cytokinesis-block micronucleus cytome assay was conducted on the per...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - July 6, 2018 Category: Genetics & Stem Cells Source Type: research

Inter strand crosslinks in DNA induced in vivo by percutaneous application of sulphur mustard to rats and mice
Publication date: August 2018Source: Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Volumes 832–833Author(s): Martina Richterova, Rudolf Stetina, Petr Jost, Hana Svobodova, Vit Rehacek, Jiri KassaAbstractInter-strand crosslinks (ICL) in the DNA are regarded to be the main toxic lesions induced by sulphur mustard (SM). We have followed the induction of ICL in the DNA of different organs of Wistar rats and Balb/c or NMRI mice by the percutaneous application of SM using the modified (reverse) comet assay. Significant amounts of ICL were found in Balb/C lymphocytes, in bone marrow and liver cells aft...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - July 6, 2018 Category: Genetics & Stem Cells Source Type: research

Protective effect of exogenous melatonin in rats and their offspring on the genotoxic response induced by the chronic consumption of alcohol during pregnancy
This study evaluated the protective effect of exogenous melatonin in rats and their offspring on the genotoxic response induced by chronic alcohol consumption during pregnancy. Twenty-five pregnant rats were divided into the following groups: NC - Negative control; ET - Rats receiving ethanol (3 g/kg/day); ET+10 M - Rats receiving ethanol (3 g/kg/day) and melatonin (10 mg/kg/day); ET+15 M - Rats receiving ethanol (3 g/kg/day) and melatonin (15 mg/kg/day); PC - Positive control (40 mg/kg cyclophosphamide). The dams and 10 pups (five males and five females) from each group were anesthetized to collect blood a...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - July 6, 2018 Category: Genetics & Stem Cells Source Type: research

Monomethylarsonous acid: Induction of DNA damage and oxidative stress in mouse natural killer cells at environmentally-relevant concentrations
Publication date: August 2018Source: Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Volumes 832–833Author(s): Huan Xu, Xiaolei Wang, Wei WangAbstractThe carcinogenicity of arsenic has been confirmed in many studies, but its mechanism of action is still unclear. A lymphocyte component of the innate immune system, natural killer (NK) cells are responsible for killing cancer cells. Although inorganic arsenical species are the prevalent forms of arsenic in the environment, monomethylarsonous acid (MMA+3) is the major arsenical species found in immune-system cells, in this 30 d drinking-water-exposure...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - July 6, 2018 Category: Genetics & Stem Cells Source Type: research

Detrimental effects detected in exfoliated buccal cells from anesthesiology medical residents occupationally exposed to inhalation anesthetics: an observational study
Publication date: Available online 3 July 2018 Source:Mutation Research/Genetic Toxicology and Environmental Mutagenesis Author(s): Mariana G. Braz, Kátina M. Souza, Lorena M.C. Lucio, Giulia C.C. Di Renzo, Luciana M. Feliciano, João Paulo C. Marcondes, C.-Y. Oliver Chen, José Reinaldo C. Braz, Leandro G. Braz Operating room professionals are scarcely aware of their individual occupational exposure to waste anesthetic gases (WAGs). Medical residents spend several hours per day in operating rooms and consequently experience occupational exposure to WAGs. Considering that no studies have yet evaluated t...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - July 4, 2018 Category: Genetics & Stem Cells Source Type: research

Protective effects of Neocuproine copper chelator against oxidative damage in NSC34 cells
Publication date: Available online 25 June 2018 Source:Mutation Research/Genetic Toxicology and Environmental Mutagenesis Author(s): Emilene A. Nunes, Tânia M. Manieri, Andreza C. Matias, Fernanda R. Bertuchi, Daniela A. da Silva, Larissa Lago, Roseli H. Sato, Giselle Cerchiaro In this work, we aim to provide evidence for the protective effect of a copper chelator, neocuproine (NeoCu), against the oxidative stress in NSC34 cells, which inhibits biomolecule oxidation and cell death. Results obtained with the comet assay allowed to determine the increase in oxidized purines and pyrimidines by H2O2 exposure, and their ...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - June 26, 2018 Category: Genetics & Stem Cells Source Type: research

Protective effect of exogenous melatonin in rats and their offspring on the genotoxic response induced by the chronic consumption of alcohol during pregnancy
This study evaluated the protective effect of exogenous melatonin in rats and their offspring on the genotoxic response induced by chronic alcohol consumption during pregnancy. Twenty-five pregnant rats were divided into the following groups: NC - Negative control; ET - Rats receiving ethanol (3 g/kg/day); ET+10 M - Rats receiving ethanol (3 g/kg/day) and melatonin (10 mg/kg/day); ET+15 M - Rats receiving ethanol (3 g/kg/day) and melatonin (15 mg/kg/day); PC - Positive control (40 mg/kg cyclophosphamide). The dams and 10 pups (five males and five females) from each group were anesthetized to collect blood a...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - June 22, 2018 Category: Genetics & Stem Cells Source Type: research

Telomere shortening associated with increased levels of oxidative stress in sulfur mustard-exposed Iranian veterans
In this study, lymphocytic telomere lengths and serum levels of isoprostane F2α were measured using q-PCR and enzyme immunoassay-based methods in 40 Iranian veterans who had been exposed to SM between 1983-88 and 40 non-exposed healthy volunteers. The relative telomere length in SM-exposed individuals was found to be significantly shorter than the non-exposed individuals. In addition, the level of 8-isoprostane F2α was significantly higher in the SM-exposed group compared to controls. Oxidative stress can be caused by defective antioxidant responses following gene mutations or altered activities of antioxidant ...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - June 21, 2018 Category: Genetics & Stem Cells Source Type: research

Modulation of chromatin conformation by the histone deacetylase inhibitor trichostatin A promotes the removal of radiation-induced lesions in ataxia telangiectasia cell lines
Publication date: Available online 15 June 2018 Source:Mutation Research/Genetic Toxicology and Environmental Mutagenesis Author(s): Alessandra Egidi, Silvia Filippi, Federico Manganello, Wilner Lopez-Martinez, Roberta Meschini Ataxia telangiectasia is a rare autosomal recessive genome instability syndrome caused by mutations in the Ataxia Telangiectasia Mutated gene and characterized by a very high sensitivity to agents inducing double strand breaks such as ionizing radiation. In cells derived from ataxia telangiectasia patients a prominent enhancement of chromosomal aberrations is revealed as a consequence of this radio...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - June 16, 2018 Category: Genetics & Stem Cells Source Type: research

In Memory of Professor Adayapalam T Natarajan
Publication date: Available online 7 May 2018 Source:Mutation Research/Genetic Toxicology and Environmental Mutagenesis Author(s): Adayabalam S. Balajee, Fabrizio Palitti, Jordi Surrallés, M. Prakash Hande (Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis)
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - June 9, 2018 Category: Genetics & Stem Cells Source Type: research

Evaluation of resveratrol radiomodifying potential for radiotherapy treatment
Publication date: Available online 8 June 2018 Source:Mutation Research/Genetic Toxicology and Environmental Mutagenesis Author(s): Yuliana C. Banegas, Eliana E. Ocolotobiche, Gisel Padula, Elisa E. Córdoba, Eduardo Fernández, Alba M. Güerci Radiotherapy is widely used for cancer treatment. However, its adverse effects that may develop during the course of treatment have forced to search agents to protect biological systems against the deleterious effects of radiation. Resveratrol (3,4,5-trihydroxy-trans-stilbene; RSV) is a natural polyphenol currently promoted for its beneficial pleiotropic effects on ...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - June 9, 2018 Category: Genetics & Stem Cells Source Type: research

Assessment of genomic instability and proliferation index in cultured lymphocytes of patients with Down Syndrome, Congenital Anomalies and Aplastic Anaemia
Publication date: Available online 8 June 2018 Source:Mutation Research/Genetic Toxicology and Environmental Mutagenesis Author(s): Alex George, Shriram Venkatesan, Narmada Ashok, Radha Saraswathy, M Prakash Hande One hundred and fifteen cases [Down Syndrome (DS) n = 75, Multiple Congenital Anomalies (MCA) n = 15 and Aplastic Anaemia (AA) n = 25], with respect to their nature of predisposition to cancer, were selected for clinical, cytogenetic and cyto-molecular studies to understand the severity of genomic instability according to the nature of the different diseases. Cytogenetic studies included chromosomal ...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - June 9, 2018 Category: Genetics & Stem Cells Source Type: research

Increased genetic damage found in waste picker women in a landfill in Paraguay measured by comet assay and the micronucleus test
Publication date: Available online 7 June 2018 Source:Mutation Research/Genetic Toxicology and Environmental Mutagenesis Author(s): Deidamia Franco de Diana, Jaime Segovia Abreu, Diana Castiglioni Serafini, José Francisco Ortíz, María José Samaniego, Ana Carina Aranda, Enrique Zamorano-Ponce Cateura landfill located in Asunción-Paraguay is the site of final disposal of tons of garbage of all kinds coming from urban, industrial and commercial areas. Due to an inadequate waste management system, there is a big concern in our country on how it can affect people working within the landfill a...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - June 8, 2018 Category: Genetics & Stem Cells Source Type: research

Inter strand crosslinks in DNA induced in vivo by percutaneous application of sulphur mustard to rats and mice
Publication date: Available online 7 June 2018 Source:Mutation Research/Genetic Toxicology and Environmental Mutagenesis Author(s): Martina Richterova, Rudolf Stetina, Petr Jost, Hana Svobodova, Vit Rehacek, Jiri Kassa Inter-strand crosslinks (ICL) in the DNA are regarded to be the main toxic lesions induced by sulphur mustard (SM). We have followed the induction of ICL in the DNA of different organs of Wistar rats and Balb/c or NMRI mice by the percutaneous application of SM using the modified (reverse) comet assay. Significant amounts of ICL were found in Balb/C lymphocytes, in bone marrow and liver cells after the dose...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - June 8, 2018 Category: Genetics & Stem Cells Source Type: research

Evaluat ıon of Health Effects, Genetıc Damage and Telomere length ın Children Exposed to Arsenic in West Bengal, İndia
Publication date: Available online 7 June 2018 Source:Mutation Research/Genetic Toxicology and Environmental Mutagenesis Author(s): Debmita Chatterjee, Shanta Adak, Nilanjana Banerjee, Pritha Bhattacharjee, Apurba K. Bandyopadhyay, Ashok K. Giri Increasing evidence of arsenic contamination in ground water and its associated adverse health outcomes affects millions of people worldwide. However, arsenic toxicity studies in children have gained impetus very recently due to the non-prominence of the hallmarks of arsenic toxicity i.e skin lesions. We recognized the need to evaluate the status of genetic damage brought about by...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - June 8, 2018 Category: Genetics & Stem Cells Source Type: research

Single nucleotide polymorphisms in ATM, TNF- α and IL6 genes and risk of radiotoxicity in breast cancer patients
Publication date: Available online 7 June 2018 Source:Mutation Research/Genetic Toxicology and Environmental Mutagenesis Author(s): Elisa E. Córdoba, Ezequiel Lacunza, Martín C. Abba, Eduardo Fernández, Alba M. Güerci Although oncological therapies have improved in the last decades, breast cancer (BC) remains a serious health problem worldwide. Radiotherapy (RT) is one of the most frequently used treatments for cancer aimed at eliminating tumor cells. However, it can also alter the surrounding normal tissue, especially the skin, and patient reactions may vary as a result of extrinsic and intrinsi...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - June 8, 2018 Category: Genetics & Stem Cells Source Type: research

Effects of age and gender on the baseline and 2 Gy 60Co γ-ray-induced nucleoplasmic bridges frequencies in the peripheral blood lymphocytes of Chinese population
Publication date: Available online 8 June 2018 Source:Mutation Research/Genetic Toxicology and Environmental Mutagenesis Author(s): Tian-Jing Cai, Xue Lu, Xue-Lei Tian, Hua Zhao, Shuang Li, Jiang-Bin Feng, De-Qing Chen, Mei Tian, Qing-Jie Liu Baseline nucleoplasmic bridges (NPB) vary widely in the general population from different regions in the same country or from different countries. The baseline NPB level in the normal Chinese population and the factors affecting the baseline and radiation-induced NPB levels have not been explored yet. The cytokinesis-block micronucleus cytome assay was conducted on the peripheral blo...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - June 8, 2018 Category: Genetics & Stem Cells Source Type: research

GENETIC DAMAGE IN ENVIRONMENTALLY EXPOSED POPULATIONS TO OPEN-PIT COAL MINING RESIDUES: ANALYSIS OF BUCCAL MICRONUCLEUS CYTOME (BMN-cyt) ASSAY AND ALKALINE, ENDO III AND FPG HIGH-THROUGHPUT COMET ASSAY
This study aimed to evaluate DNA damage using the cytome assay (BMN-cyt) in buccal cells and its relation to primary and oxidative DNA damage in lymphocytes, assessed by the high-throughput alkaline and modified (FPG-ENDO III) Comet assay in individuals with environmental exposure to coal mining residues in northern Colombia. Considering metals from coal mining activities as the main source of reactive oxygen species (ROS) generation, the concentrations of inorganic elements in blood samples was also assessed. The analysis revealed that frequencies of BMN-cyt parameters related to DNA damage (micronuclei), cytokinesis (bin...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - June 5, 2018 Category: Genetics & Stem Cells Source Type: research

γH2AX prefers late replicating metaphase chromosome regions
Publication date: Available online 4 June 2018 Source:Mutation Research/Genetic Toxicology and Environmental Mutagenesis Author(s): Ana Laura Reyes-Ábalos, Liddle Pablo, Gustavo A. Folle, María Vittoria Di Tomaso DNA damage response (DDR) constitutes a protein pathway to handle eukaryotic DNA lesions in the context of chromatin. DDR engages the recruitment of signaling, transducer, effector, chromatin modifiers and remodeling proteins, allowing cell cycle delay, DNA repair or induction of senescence or apoptosis. An early DDR-event includes the epigenetic phosphorylation of the histone variant H2AX on serine...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - June 5, 2018 Category: Genetics & Stem Cells Source Type: research

Caliphruria subedentata (Amaryllidaceae) decreases genotoxicity and cell death induced by β-amyloid peptide in sh-sy5y cell line
Publication date: Available online 2 June 2018 Source:Mutation Research/Genetic Toxicology and Environmental Mutagenesis Author(s): Willian Orlando Castillo, Andres Felipe Aristizabal-Pachon, Elsa Sakamoto-Hojo, Cristian Aldemar Gasca, Fabio Antonio Cabezas-Fajardo, Catarina Takahashi Alzheimer’s disease (AD) is a neurodegenerative disorder characterized by neuritic plaques (NPs), and neurofibrillary tangles (NFTs). β-Amyloid peptide1-42 (Aβ(1-42)) is the principal component of NPs and is associated with oxidative stress, as well as dysfunction of cholinergic neurotransmission system and cell death. Nevert...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - June 3, 2018 Category: Genetics & Stem Cells Source Type: research

Evaluation of the genotoxic properties of nickel oxide nanoparticles in vitro and in vivo
Publication date: Available online 2 June 2018 Source:Mutation Research/Genetic Toxicology and Environmental Mutagenesis Author(s): Raíne F. De Carli, Débora N. Chaves, Tatiane R. Cardozo, Ana Paula de Souza, Allan Seeber, Wladimir H. Flores, Karol F. Honatel, Mauricio Lehmann, Rafael R. Dihl Nickel-based nanoparticles (NPs) are new products with an increasing number of industrial applications that were developed in recent years. NiO NPs are present in several nanotechnological industrial products, and the characterization of their genotoxic potential is essential. The present study assessed the genotoxicity...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - June 3, 2018 Category: Genetics & Stem Cells Source Type: research

BCL2 AND MIR-181a TRANSCRIPTIONAL ALTERATIONS IN UMBILICAL-CORD BLOOD CELLS CAN BE PUTATIVE BIOMARKERS FOR OBESITY
In conclusion, our study demonstrated that the GDM hyperglycemic intrauterine environment promotes transcriptional alterations in BCL2 and miR-181a in neonate umbilical-cord blood cells. Furthermore, both GDM neonates and obese subjects share the same transcriptional alteration in BCL2. Considering the relationship between obesity development and the functions regulated by these two genes, BCL2 and miR-181a could be adopted as potential biomarkers for childhood obesity. However, further study designs are recommended to confirm this hypothesis. (Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis)
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - June 3, 2018 Category: Genetics & Stem Cells Source Type: research

Simulation of mouse and rat spermatogenesis to inform genotoxicity testing using oecd test guideline 488
Publication date: Available online 1 June 2018 Source:Mutation Research/Genetic Toxicology and Environmental Mutagenesis Author(s): Francesco Marchetti, Marilyn Aardema, Carol Beevers, Jan van Benthem, George R. Douglas, Roger Godschalk, Carole L. Yauk, Robert Young, Andrew Williams The Organisation for Economic Co-operation and Development Test Guideline (TG) 488 for the transgenic rodent (TGR) mutation assay recommends two sampling times for assessing germ cell mutagenicity following the required 28-day exposure period: 28 + > 49 days for mouse sperm and 28 + >70 days for rat sperm from the c...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - June 2, 2018 Category: Genetics & Stem Cells Source Type: research

In vivo antigenotoxic activity of Diplotaxis tenuifolia against cyclophosphamide-induced DNA damage: relevance of modulation of hepatic ABC efflux transporters
Publication date: Available online 1 June 2018 Source:Mutation Research/Genetic Toxicology and Environmental Mutagenesis Author(s): Marcela M. López Nigro, Roxana N. Peroni, Iván Ayllón-Cabrera, Victoria E. Schiariti Lampropulos, Martín I. Roma, Marta A. Carballo Several epidemiological studies have demonstrated that a diet with high contents of cruciferous vegetables (which belong to the Brassicaceae family) may reduce the incidence of cancer and neurodegenerative diseases. However, some authors have postulated that they might bring about toxic effects. Therefore, the aim of this study was to ...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - June 2, 2018 Category: Genetics & Stem Cells Source Type: research

DNA Damage and repair in individuals with ataxia-telangiectasia and their parents
Publication date: Available online 1 June 2018 Source:Mutation Research/Genetic Toxicology and Environmental Mutagenesis Author(s): Roberta Passos Palazzo, Laura Bannach Jardim, Alexandre Bacellar, Fernanda Ramos de Oliveira, Flora Troina Maraslis, Carolina Hilgert Jacobsen Pereira, Juliana da Silva, Sharbel Weidner Maluf Ataxia-telangiectasia (AT) is a multisystem, complex and rare disease inherited in an autosomal recessive manner. Homozygous individuals have a variety of pathological manifestations, however, heterozygotes only present a higher risk of developing cancer. We evaluated the background levels of DNA damage ...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - June 2, 2018 Category: Genetics & Stem Cells Source Type: research

Polymorphisms in IMPDH2, UGT2B7, and CES2 genes influence the risk of graft rejection in kidney transplant recipients taking mycophenolate mofetil
Publication date: Available online 1 June 2018 Source:Mutation Research/Genetic Toxicology and Environmental Mutagenesis Author(s): Heloísa Lizotti Cilião, Rossana Batista Oliveira Camargo-Godoy, Marilesia Ferreira de Souza, Amanda Zanuto, Vinicius Daher Alvares Delfino, Ilce Mara de Syllos Cólus The immunosuppressant mycophenolic acid (MPA), derived from the prodrug mycophenolate mofetil (MMF), is a drug used widely by kidney transplant recipients. This drug selectively inhibits inosine monophosphate dehydrogenase that controls the proliferation of lymphocytes, aiding in the prevention of rejection o...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - June 2, 2018 Category: Genetics & Stem Cells Source Type: research

Expression patterns and mutation analysis of p53 in fish Rita rita from polluted riverine environment
Publication date: Available online 31 May 2018 Source:Mutation Research/Genetic Toxicology and Environmental Mutagenesis Author(s): Tandrima Mitra, Bimal Prasanna Mohanty, Sasmita Mohanty, Gopal Krishna Purohit, Basanta Kumar Das The present study was undertaken to investigate the alterations in gene expression patterns and for mutation analysis of p53 in the riverine catfish Rita rita collected from polluted riverine habitat. The partial p53 gene sequence of Rita rita generated showed a high degree of similarities with the DNA binding domains of fishes, mice and human. Transcriptomic analysis, carried out by quantitative...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - June 1, 2018 Category: Genetics & Stem Cells Source Type: research