Mutation Research Genetic Toxicology and Environmental Mutagenesis 
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This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.DNA damage and repair measured by comet assay in cancer patients
Publication date: Available online 20 May 2019Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Pavel Vodicka, Sona Vodenkova, Alena Opattova, Ludmila VodickovaAbstractThe last decade witnessed an increase in the use of comet assay for DNA damage monitoring in cancer patients and controls. Apart from case-control studies, reports described the determination of DNA damage prior to (baseline value) and after chemo-/radiotherapy, the treatment resulted in significantly elevated DNA damage. However, studies on DNA damage as a factor reflecting cancer prognosis and therapy prediction are scarc...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - May 22, 2019 Category: Genetics & Stem Cells Source Type: research
Mycoplasma infection of cultured cells induces oxidative stress and attenuates cellular base excision repair activity
In conclusion, this study showed that not only does Mycoplasma infection induce oxidative stress and DNA damage, but it also decreases the efficiency of the main pathway responsible for the repair of oxidatively damaged DNA i.e. BER. In this in vitro model, there is no mechanism for infection-induced inflammation, which could be a source of increased ROS production. Therefore, further studies are needed to evaluate how Mycoplasma infection causes oxidatively damaged DNA, and how it modulates cellular DNA repair. (Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis)
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - May 22, 2019 Category: Genetics & Stem Cells Source Type: research
Evaluation of a 28-day repeated-dose micronucleus test in rat glandular stomach, colon, and liver using gastrointestinal tract-targeted genotoxic-carcinogens and non-carcinogens
Publication date: Available online 19 May 2019Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Emiko Okada, Yohei Fujiishi, Kazunori Narumi, Shoichi Kado, Wakako OhyamaAbstractThe usefulness of the rat repeated-dose liver and gastrointestinal (GI) tract micronucleus (MN) tests to detect site-specific carcinogens has been shown previously using 22 chemicals in a study conducted by the Mammalian Mutagenicity Study group in the Japanese Environmental Mutagen Society. However, in the 6th International Workshop on Genotoxicity Testing, the need for further data to identify the sensitivity and...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - May 20, 2019 Category: Genetics & Stem Cells Source Type: research
The Test that Changed the World: The Ames Test and the Regulation of Chemicals
Publication date: Available online 15 May 2019Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Errol ZeigerAbstractThe bacterial strains and mutagenicity test procedure developed by Bruce Ames, and published in 1973, greatly enhanced the ability of laboratories to test chemicals for mutagenicity. The test that became known as the “Ames Test” was simple to perform, took only two days, was relatively inexpensive, and was easily transferrable to other laboratories. Their demonstration that the test was effective at identifying potentially carcinogenic chemicals led its immediate adoptio...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - May 16, 2019 Category: Genetics & Stem Cells Source Type: research
Concentration-response studies of the chromosome-damaging effects of topoisomerase II inhibitors determined in vitro using human TK6 cells
Publication date: Available online 15 May 2019Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): P. Gollapudi, V.S. Bhat, D.A. EastmondAbstractTopoisomerase II (topo II) inhibitors are commonly used as chemotherapy to treat multiple types of cancer, though their use is also associated with the development of therapy related acute leukemias. While the chromosome-damaging effects of etoposide, a topo II poison, have been proposed to act through a threshold mechanism, little is known about the chromosome damaging effects and dose responses for the catalytic inhibitors of the enzyme. The curre...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - May 16, 2019 Category: Genetics & Stem Cells Source Type: research
The application of an in vitro micronucleus test in mouse fibroblast L929 cells
Publication date: Available online 11 May 2019Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Ewa Drozd, Irena Bubko, Karolina Jaworska, Beata M. Gruber-BzuraAbstractThe MNa (in vitro the micronucleus assay) is recommended for studying genotoxicity of chemicals. However, no protocol is currently available for experiments with mouse fibroblast L929 cells. The aim of this study was to improve the scope of CBMNb (cytokinesis-block micronucleus) test. Optimization consisted of: selection of a non-cytotoxic concentration of cytokinesis blocker - cytoBc (cytochalasin B) and type and definitio...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - May 12, 2019 Category: Genetics & Stem Cells Source Type: research
Comet assay in ecogenotoxicology: applications in Mytilus sp
Publication date: Available online 8 May 2019Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Claudia Bolognesi, Silvia Cirillo, James Kevin ChipmanAbstractThe comet assay is a sensitive technique to detect DNA damage caused by exposure to genotoxic chemical and physical agents and is widely used in ecotoxicology. The assay has been applied in aquatic species, mainly fish and bivalves, in field biomonitoring programs and in experimental studies. The aim of the present study was to retrieve and review the published evidence to define the role of the comet assay in the assessment of genoto...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - May 10, 2019 Category: Genetics & Stem Cells Source Type: research
Oxidative stress and Endoreduplication induced by blue light exposure to CHO cells
In conclusion, exposure to blue light resulted in significant genotoxicity of the treated cells. (Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis)
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - May 5, 2019 Category: Genetics & Stem Cells Source Type: research
Impact of the glyphosate-based commercial herbicide, its components and its metabolite AMPA on non-target aquatic organisms
Publication date: Available online 4 May 2019Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Laís de Brito Rodrigues, Gessyca Gonçalves Costa, Emanoela Lundgren Thá, Lucas Rafael da Silva, Rhaul de Oliveira, Daniela Morais Leme, Marta Margarete Cestari, Cesar Koppe Grisolia, Marize Campos Valadares, Gisele Augusto Rodrigues de OliveiraAbstractGlyphosate (GLY) is the active ingredient of several herbicide formulations widely used to control weeds in agricultural and non-agricultural areas. Due to the intensive use of GLY-based herbicides and their direct application on soils, some of ...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - May 5, 2019 Category: Genetics & Stem Cells Source Type: research
Titanium dioxide nanoparticles tested for genotoxicity with the comet and micronucleus assays in vitro, ex vivo and in vivo
Publication date: Available online 2 May 2019Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Alena Kazimirova, Magdalena Barancokova, Marta Staruchova, Martina Drlickova, Volkovova Katarina, Dusinska MariaAbstractThe genotoxicity of TiO2 nanoparticles (NPs) was assessed with the cytokinesis-block micronucleus (CBMN) assay in TK6 lymphoblastoid cells, lymphocytes from human volunteers, and bone marrow erythrocytes from rats exposed in vivo; and with the comet assay (detecting both strand breaks and oxidised purines) in human and rat peripheral blood mononuclear cells (PBMCs). NPs were di...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - May 3, 2019 Category: Genetics & Stem Cells Source Type: research
Role of aneuploidy in the carcinogenic process: Part 3 of the report of the 2017 IWGT workgroup on assessing the risk of aneugens for carcinogenesis and hereditary diseases
Publication date: Available online 29 March 2019Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): David Tweats, David A. Eastmond, Anthony M. Lynch, Azeddine Elhajouji, Roland Froetschl, Micheline Kirsch-Volders, Francesco Marchetti, Kenichi Masumura, Francesca Pacchierotti, Maik SchulerAbstractAneuploidy is regarded as a hallmark of cancer, however, its role is complex with both pro- and anti-carcinogenic effects evident. In this IWGT review, we consider the role of aneuploidy in cancer biology; cancer risk associated with constitutive aneuploidy; rodent carcinogenesis with known chemica...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - May 2, 2019 Category: Genetics & Stem Cells Source Type: research
Technical recommendations to perform the alkaline standard and enzyme-modified comet assay in human biomonitoring studies
Publication date: Available online 1 May 2019Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Amaya Azqueta, Damian Muruzabal, Elisa Boutet-Robinet, Mirta Milic, Maria Dusinska, Gunnar Brunborg, Peter Møller, Andrew R. CollinsAbstractThe comet assay (single cell gel electrophoresis) is widely used as a biomonitoring tool to assess DNA damage - strand breaks, as well as oxidised bases; it can also be adapted to measure DNA repair. It is based on the ability of breaks in the DNA to relax supercoiling, allowing DNA loops to extend from the nuclear core (nucleoid) under an electric field to...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - May 2, 2019 Category: Genetics & Stem Cells Source Type: research
A perspective on the development of the Ames Salmonella/mammalian-microsome mutagenicity assay
Publication date: May 2019Source: Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Volume 841Author(s): Kristien MortelmansAbstractThis Virtual Special Issue of Mutation Research is dedicated to Professor Bruce N. Ames in recognition of his 90th birthday in December 2018. His pioneering work in the field of chemical mutagenesis resulted in the well-known Ames Salmonella/mammalian-microsome mutagenicity assay that has played a pivotal role since the 1970s in the field of genetic toxicology. The assay is usually referred to as the Ames test and was gradually developed by improving the sensitivity of the te...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - April 28, 2019 Category: Genetics & Stem Cells Source Type: research
Characterization of urban aerosol: seasonal variation of genotoxicity of the water-soluble portion of PM2.5 and PM1
This study also shows that the water-soluble fraction of PM contributes to global genotoxicity and that transition metals play a role, therefore both organic and water-soluble fractions should be considered in an air-quality monitoring program. (Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis)
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - April 27, 2019 Category: Genetics & Stem Cells Source Type: research
Chronic Occupational Noise Exposure: Effects on DNA Damage, Blood Pressure, and Serum Biochemistry
Publication date: Available online 26 April 2019Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Majid Bagheri Hosseinabadi, Narges Khanjani, Thomas Münzel, Andreas Daiber, Mohammad YaghmorlooAbstractNoise levels experienced by industrial workers may cause both auditory and non-auditory harmful effects. We have studied the effect of chronic industrial noise exposure on DNA damage, blood pressure, and serum biochemistry in factory workers. Male workers (109 individuals) in three parts of a food factory in Shahroud, Iran were enrolled as the exposed group and male office workers (123 indi...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - April 27, 2019 Category: Genetics & Stem Cells Source Type: research
