Circ-Bptf Ameliorates Learning and Memory Impairments via the miR-138-5p/p62 Axis in APP/PS1 Mice
AbstractAlzheimer's disease (AD) is a common age-associated progressive neurodegenerative disorder that is implicated in the aberrant regulation of numerous circular RNAs (circRNAs). Here, we reported that circ-Bptf, a conserved circRNA derived from the Bptf gene, showed an age-dependent decrease in the hippocampus of APP/PS1 mice. Overexpression of circ-Bptf significantly reversed dendritic spine loss and learning and memory impairment in APP/PS1 mice. Moreover, we found that circ-Bptf was predominantly localized to the cytoplasm and upregulated p62 expression by binding to miR-138-5p. Furthermore, the miR-138-5p mimics r...
Source: Molecular Neurobiology - March 25, 2024 Category: Neurology Source Type: research
Correction: Anandamide and WIN 55212 –2 Afford Protection in Rat Brain Mitochondria in a Toxic Model Induced by 3-Nitropropionic Acid: an In Vitro Study
(Source: Molecular Neurobiology)
Source: Molecular Neurobiology - March 25, 2024 Category: Neurology Source Type: research
Identification of Anoikis-Related Genes in Spinal Cord Injury: Bioinformatics and Experimental Validation
In this study, we aimed to identify the key anoikis-related genes (ARGs) providing therapeutic targets for SCI. The mRNA expression matrix of GSE45006 was downloaded from the Gene Expression Omnibus (GEO) database, and the ARGs were downloaded from the Molecular Signatures Database (MSigDB database). Then, the potential differentially expressed ARGs were identified. Next, correlation analysis, gene ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis, and protein –protein interaction (PPI) analysis were employed for the differentially expressed ARGs. Moreover, m...
Source: Molecular Neurobiology - March 23, 2024 Category: Neurology Source Type: research
NUS1 Variants Cause Lennox-Gastaut Syndrome Related to Unfolded Protein Reaction Activation
In this study, we identified twode novo variants ofNUS1, a missense variant (c.868 C > T/p.R290C) and a splice site variant (c.792-2 A > G), in two unrelated LGS patients using trio-based whole-exome sequencing performed in a cohort of 165 LGS patients. Both variants were absent in the gnomAD population and showed a significantly higher observed number of variants than expected genome-wide. The R290C variant was predicted to damag e NUS1 and decrease its protein stability. The c.792-2 A > G variant caused premature termination of the protein. Knockdown ofNUS1 activated the UPR pathway, resulting...
Source: Molecular Neurobiology - March 23, 2024 Category: Neurology Source Type: research
Parkinson ’s Disease and MicroRNAs: A Duel Between Inhibition and Stimulation of Apoptosis in Neuronal Cells
AbstractParkinson ’s disease (PD) is one of the most prevalent diseases of central nervous system that is caused by degeneration of the substantia nigra’s dopamine-producing neurons through apoptosis. Apoptosis is regulated by initiators’ and executioners’ caspases both in intrinsic and extrinsic pathways, fu rther resulting in neuronal damage. In that context, targeting apoptosis appears as a promising therapeutic approach for treating neurodegenerative diseases. Non-coding RNAs—more especially, microRNAs, or miRNAs—are a promising target for the therapy of neurodegenerative diseases because they ar e essentia...
Source: Molecular Neurobiology - March 23, 2024 Category: Neurology Source Type: research
Utilizing Machine Learning to Identify Biomarkers of Endoplasmic Reticulum Stress and Analyze Immune Cell Infiltration in Parkinson ’s Disease
The objective of this investigation was to examine the relationship between immune system infiltration, ATP-binding cassette transporter subfamily A member 7 (ABCA7) and TBL2 as well as potential therapeutic targets for the identification of PD associated to en doplasmic reticulum (ER) stress. First, we obtained PD data through GEO and divided it into two sets: a training set (GSE8397) plus a set for validation (GSE7621). Functional enrichment analysis was performed on a set of DEGs that overlapped with genes involved in endoplasmic reticulum stress. To id entify genes of PD linked with endoplasmic reticulum stress, we emp...
Source: Molecular Neurobiology - March 23, 2024 Category: Neurology Source Type: research
Resveratrol Ameliorates Retinal Ischemia-Reperfusion Injury by Modulating the NLRP3 Inflammasome and Keap1/Nrf2/HO-1 Signaling Pathway
In conclusion, our results discovered that Res administration showed neuroprotective effects through inhibition of the NLRP3 inflammasome pathway and activation of Keap1/Nrf2/HO-1 pathway. Thus, we further elucidated the potential of Res in glauco ma therapy. (Source: Molecular Neurobiology)
Source: Molecular Neurobiology - March 22, 2024 Category: Neurology Source Type: research
Roles of KCNA2 in Neurological Diseases: from Physiology to Pathology
AbstractPotassium voltage-gated channel subfamily a member 2 (Kv1.2, encoded byKCNA2) is highly expressed in the central and peripheral nervous systems. Based on the patch clamp studies, gain-of function (GOF), loss-of-function (LOF), and a mixed type (GOF/LOF) variants can cause different conditions/disorders.KCNA2-related neurological diseases include epilepsy, intellectual disability (ID), attention deficit/hyperactive disorder (ADHD), autism spectrum disorder (ASD), pain as well as autoimmune and movement disorders. Currently, the molecular mechanisms for the reported variants in causing diverse disorders are unknown. ...
Source: Molecular Neurobiology - March 22, 2024 Category: Neurology Source Type: research
Acute Administration of HIV-1 Tat Protein Drives Glutamatergic Alterations in a Rodent Model of HIV-Associated Neurocognitive Disorders
AbstractHIV-1-associated neurocognitive disorders (HAND) are a major comorbidity of HIV-1 infection, marked by impairment of executive function varying in severity. HAND affects nearly half of people living with HIV (PLWH), with mild forms predominating since the use of anti-retroviral therapies (ART). The HIV-1 transactivator of transcription (Tat) protein is found in the cerebrospinal fluid of patients adherent to ART, and its administration or expression in animals causes cognitive symptoms. Studies of Tat interaction with theN-methyl-D-aspartate receptor (NMDAR) suggest that glutamate toxicity contributes to Tat-induce...
Source: Molecular Neurobiology - March 22, 2024 Category: Neurology Source Type: research
AQP4 Endocytosis-Lysosome Degradation Mediated by MMP-9/ β-DG Involved in Diabetes Cognitive Impairment
In this study, we observed that diabetes mice displayed cognitive disorder accompanied by reduction of AQP4 in prefrontal cortex. And we found that bafilomycin A1, a widely used lysosome inhibitor, could reverse the downregulat ion of AQP4 in diabetes, further demonstrating that the reduction of AQP4 in diabetes is a result of more endocytosis-lysosome degradation. In further experiments, we found diabetes caused the excessive activation of MMP-9/β-DG which leaded to the loss of connection between AQP4 and β-DG, further inducing the endocytosis of AQP4. Moreover, inhibition of MMP-9/β-DG restored the endocytosis-lysosom...
Source: Molecular Neurobiology - March 21, 2024 Category: Neurology Source Type: research
LPAR6 Participates in Neuropathic Pain by Mediating Astrocyte Cells via ROCK2/NF- κB Signal Pathway
In this study, we established a mouse model of chronic constriction injury (CCI) to simulate NPP. It was found that the expression of LPAR6 in AS cells of the spinal dorsal horn was increased in the CCI model, and the thresholds of mechanical and thermal pain were elevated after knocking out LPAR6, indicating that LPAR6 and AS cells participated in the occurrence of NPP. The experiment involved culturing primary AS cells and knocking down LPAR6 by Lentivirus. The results showed that the NF- κB signal pathway was activated and the number of A1-type AS cells increased in the CCI model. However, LPAR6 knockdown inhibited the...
Source: Molecular Neurobiology - March 20, 2024 Category: Neurology Source Type: research
Research Advances in Neuroblast Migration in Traumatic Brain Injury
AbstractNeuroblasts were first derived from the adult mammalian brains in the 1990s by Reynolds et al. Since then, persistent neurogenesis in the subgranular zone (SGZ) of the hippocampus and subventricular zone (SVZ) has gradually been recognized. To date, reviews on neuroblast migration have largely investigated glial cells and molecular signaling mechanisms, while the relationship between vasculature and cell migration remains a mystery. Thus, this paper underlines the partial biological features of neuroblast migration and unravels the significance and mechanisms of the vasculature in the process to further clarify the...
Source: Molecular Neurobiology - March 20, 2024 Category: Neurology Source Type: research
High Frequency of Autoantibodies in COVID-19 Patients with Central Nervous System Complications: a Multicenter Observational Study
We present a panel of central nervous system (CNS) complications associated with coronavirus disease 2019 (COVID-19) and their clinical characteristics. We aim to investigate associations between neurological autoantibodies and COVID-19 patients with predominant CNS complications. In this retrospective multi-center study, we analyze neurologic complications associated with COVID-19 patients from Dec. 2022 to Feb. 2023 at four tertiary hospitals in China. CSF and/or serum in the enrolled patients were tested for autoantibodies using tissue-based assays (TBAs) and cell-based assays (CBAs). A total of 34 consecutive patients ...
Source: Molecular Neurobiology - March 20, 2024 Category: Neurology Source Type: research
Relationship Between Reactive Astrocytes, by [18F]SMBT-1 Imaging, with Amyloid-Beta, Tau, Glucose Metabolism, and TSPO in Mouse Models of Alzheimer ’s Disease
AbstractReactive astrocytes play an important role in the development of Alzheimer ’s disease (AD). Here, we aimed to investigate the temporospatial relationships among monoamine oxidase-B, tau and amyloid-β (Aβ), translocator protein, and glucose metabolism by using multitracer imaging in AD transgenic mouse models. Positron emission tomography (PET) imaging with [18F]SMBT-1 (monoamine oxidase-B), [18F]florbetapir (A β), [18F]PM-PBB3 (tau), [18F]fluorodeoxyglucose (FDG), and [18F]DPA-714 (translocator protein) was carried out in 5- and 10-month-old APP/PS1, 11-month-old 3 ×Tg mice, and aged-matched wild-type mice. T...
Source: Molecular Neurobiology - March 19, 2024 Category: Neurology Source Type: research
Correction: An In Vitro Study for the Role of Schizophrenia-Related Potential miRNAs in the Regulation of COMT Gene
(Source: Molecular Neurobiology)
Source: Molecular Neurobiology - March 19, 2024 Category: Neurology Source Type: research
