Independent and cooperative regulation of staphylopine biosynthesis and trafficking by Fur and Zur
Molecular Microbiology, EarlyView. (Source: Molecular Microbiology)
Source: Molecular Microbiology - February 26, 2018 Category: Microbiology Source Type: research
ClpC and MecA, components of a proteolytic machine, prevent Spo0A ‐P‐dependent transcription without degradation
Molecular Microbiology, EarlyView. (Source: Molecular Microbiology)
Source: Molecular Microbiology - February 26, 2018 Category: Microbiology Source Type: research
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Molecular Microbiology, Ahead of Print. (Source: Molecular Microbiology)
Source: Molecular Microbiology - February 26, 2018 Category: Microbiology Source Type: research
The evolutionary impact of Intragenic FliA Promoters in Proteobacteria
This article is protected by copyright. All rights reserved.
Recent findings have identified thousands of bacterial promoters in unexpected locations, such as inside genes. Here, we investigate the functions of intragenic promoters for the flagellar sigma factor FliA. Our data suggest that most of these promoters are not functional, but that one intragenic FliA promoter is broadly conserved, and constrains evolution of the overlapping protein‐coding gene. Our data suggest that intragenic regulatory sequences significantly impact bacterial genome evolution. (Source: Molecular Microbiology)
Source: Molecular Microbiology - February 25, 2018 Category: Microbiology Authors: Devon M. Fitzgerald, Carol Smith, Pascal Lapierre, Joseph T. Wade Tags: Research Article Source Type: research
Borrelia burgdorferi genes, bb0639 ‐0642, encode a putative putrescine/spermidine transport system PotABCD that is spermidine specific and essential for cell survival
This article is protected by copyright. All rights reserved.
Borrelia burgdorferi, Lyme disease agent, harbors no enzymes to synthesize or degrade polyamines yet does contain the genes encoding a putative polyamine uptake system (potABCD). Here, we demonstrated that the PotABCD is a spermidine‐specific transporter system that is essential for survival. The genes are upregulated during tick feeding concomitantly with the decrease of osmoliarity which has been shown to trigger virulence factor expression. The potABCD genes are regulated in a RpoN‐dependent, RpoS‐independent, BosR‐independent fashion. (Source: Molecular Microbiology)
Source: Molecular Microbiology - February 24, 2018 Category: Microbiology Authors: S ébastien Bontemps‐Gallo, Kevin A. Lawrence, Crystal L. Richards, Frank C. Gherardini Tags: Research Article Source Type: research
Waking the Neighbours: Disruption of H ‐NS Repression by Overlapping Transcription
This article is protected by copyright. All rights reserved.
The histone‐like nucleoid structuring (H‐NS) protein and its analogues silence transcription across large stretches of horizontally acquired AT‐rich DNA in a broad range of bacterial species. In this issue of Molecular Microbiology, Rangarajan and Schnetz show that when transcription from a neighbouring region invades an H‐NS‐bound locus, it can disrupt local H‐NS repression. This finding has important implications for H‐NS function and its impact on genome evolution. (Source: Molecular Microbiology)
Source: Molecular Microbiology - February 23, 2018 Category: Microbiology Authors: Joseph T. Wade, David C. Grainger Tags: MicroCommentary Source Type: research
Cryo ‐electron tomography analyses of terminal organelle mutants suggest the motility mechanism of Mycoplasma genitalium
This article is protected by copyright. All rights reserved.
Here, we visualized the terminal organelles of Mycoplasma genitalium deletion mutants by cellular cryo‐electron tomography. Individual protein ensembles act as springs that periodically expand and retract, causing the cell tip to move forward, while the backward movement is constrained by other proteins that act as a ratchet. This work is a rich resource of cryo‐electron tomograms that shed light into the molecular orchestration of the motility of this minimal genome organism. (Source: Molecular Microbiology)
Source: Molecular Microbiology - February 23, 2018 Category: Microbiology Authors: Anja Seybert, Luis Gonzalez ‐Gonzalez, Margot P. Scheffer, Maria Lluch‐Senar, Ana M. Mariscal, Enrique Querol, Franziska Matthaeus, Jaume Piñol, Achilleas S. Frangakis Tags: Research Article Source Type: research
Interference of transcription across H ‐NS binding sites and repression by H‐NS
Molecular Microbiology, EarlyView. (Source: Molecular Microbiology)
Source: Molecular Microbiology - February 23, 2018 Category: Microbiology Source Type: research
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Molecular Microbiology, Ahead of Print. (Source: Molecular Microbiology)
Source: Molecular Microbiology - February 23, 2018 Category: Microbiology Source Type: research
Genes affecting progression of bacteriophage P22 infection in Salmonella identified by transposon and single gene deletion screens
This article is protected by copyright. All rights reserved.
Cartoon schematic of two high throughput screens to assay genes important for P22 infection in Salmonella (Source: Molecular Microbiology)
Source: Molecular Microbiology - February 22, 2018 Category: Microbiology Authors: Kaitlynne Bohm, Steffen Porwollik, Weiping Chu, John A. Dover, Eddie B. Gilcrease, Sherwood R. Casjens, Michael McClelland, Kristin N. Parent Tags: Research Article Source Type: research
Electron Cryotomography of Mycoplasma pneumoniae Mutants Correlates Terminal Organelle Architectural Features and Function
This article is protected by copyright. All rights reserved.
Mycoplasma pneumoniae mutants defective in adherence and gliding were analyzed by electron cryotomography for corresponding changes in terminal organelle architecture. Ultrastructural changes ranged from complete loss of all eleven terminal organelle substructures in some mutants, to changes in specific substructures such as the protein knobs on the terminal organelle surface (green) or the bowl substructure (purple) at its base. Somewhat surprisingly, for other mutants no differences in terminal organelle architecture were evident. (Source: Molecular Microbiology)
Source: Molecular Microbiology - February 22, 2018 Category: Microbiology Authors: Duncan C. Krause, Songye Chen, Jian Shi, Ashley Jensen, Edward S. Sheppard, Grant J. Jensen Tags: Research Article Source Type: research
Conserved residues are critical for Haloferax volcanii archaeosortase catalytic activity: implications for convergent evolution of the catalytic mechanisms of non ‐homologous sortases from archaea and bacteria
This article is protected by copyright. All rights reserved.
Although evolutionarily unrelated, bacterial sortases and archaeal archaeosortases both recognize and process the C‐termini of substrates having similar tripartite structures, followed by covalent attachment to the cell surface via the newly generated C‐termini. Here, we show that, similar to the sortase, the archaeosortase active site contains a catalytic triad of amino acids that processes the substrates. However, in archaeosortases, but not sortases, these residues appear to be located within three conserved transmembrane segments. (Source: Molecular Microbiology)
Source: Molecular Microbiology - February 21, 2018 Category: Microbiology Authors: Mohd Farid Abdul Halim, Ronald Rodriguez, Jonathan D. Stoltzfus, Iain G. Duggin, Mechthild Pohlschroder Tags: Research Article Source Type: research
