Practical Guidance for Developing Small-Molecule Optical Probes for In Vivo Imaging
AbstractThe WMIS Education Committee (2019 –2022) reached a consensus that white papers on molecular imaging could be beneficial for practitioners of molecular imaging at their early career stages and other scientists who are interested in molecular imaging. With this consensus, the committee plans to publish a series of white papers on to pics related to the daily practice of molecular imaging. In this white paper, we aim to provide practical guidance that could be helpful for optical molecular imaging, particularly for small molecule probe development and validationin vitro andin vivo. The focus of this paper is precli...
Source: Molecular Imaging and Biology - February 6, 2023 Category: Molecular Biology Source Type: research
Editorial to the Special Issue Entitled “Optical Surgical Navigation”
(Source: Molecular Imaging and Biology)
Source: Molecular Imaging and Biology - February 2, 2023 Category: Molecular Biology Source Type: research
PEGylated and Non-PEGylated TCP-1 Probes for Imaging of Colorectal Cancer
ConclusionsTCP-1-based probes undergo endocytosis and localize in the cytoplasm and nucleus of human and mouse CRC cells. Tumor detectability of fluorescent TCP-1 peptide with a PEG4 spacer is promising due to its enhanced tumor binding affinity and rapid clearance kinetics from nontumor tissues. Non-PEGylated [99mTc]Tc-HYNIC-TCP-1 exhibits lower nonspecific accumulation in the liver and gastrointestinal tract and might have better capability for detecting CRC lesions in clinical sites. TCP-1 may represent an innovative targeting molecule for detecting CRC noninvasively. (Source: Molecular Imaging and Biology)
Source: Molecular Imaging and Biology - February 1, 2023 Category: Molecular Biology Source Type: research
Two-Photon Endoscopy: State of the Art and Perspectives
AbstractIn recent years, the demand for non-destructive deep-tissue imaging modalities has led to interest in multiphoton endoscopy. In contrast to bench top systems, multiphoton endoscopy enables subcellular resolution imaging in areas not reachable before. Several groups have recently presented their development towards the goal of producing user friendly plug and play system, which could be used in biological research and, potentially, clinical applications. We first present the technological challenges, prerequisites, and solutions in two-photon endoscopic systems. Secondly, we focus on the applications already found i...
Source: Molecular Imaging and Biology - February 1, 2023 Category: Molecular Biology Source Type: research
Halogen Replacement on the Lysine Side Chain of Lys-Urea-Glu-Based PSMA Inhibitors Leads to Significant Changes in Targeting Properties
ConclusionsIt was demonstrated that the halogen substituent on the lysine terminal amino group on the Glu-urea-Lys backbone did positively affect the binding of [68Ga]Ga-DOTA-SC691-R to PSMA. The bulkier and less electronegative Br (or I) elements are preferred for structural modifications here. (Source: Molecular Imaging and Biology)
Source: Molecular Imaging and Biology - January 25, 2023 Category: Molecular Biology Source Type: research
Comparison of Near-Infrared Imaging Agents Targeting the PTPmu Tumor Biomarker
ConclusionsThe SBK2 PTPmu-targeting peptide conjugated to ICG specifically labels heterotopic human gliomas grown in mice between 10 min and 24 h following injection. Similar molecules constructed with more hydrophilic dyes demonstrated no specificity. These studies present a promising candidate for use in FIGS of PTPmu biomarker–expressing tumors. (Source: Molecular Imaging and Biology)
Source: Molecular Imaging and Biology - January 25, 2023 Category: Molecular Biology Source Type: research
Predicting Soft Tissue Sarcoma Response to Neoadjuvant Chemotherapy Using an MRI-Based Delta-Radiomics Approach
ConclusionOverall, while our result was not able to separate NAC responders from non-responders, our analyses did identify a subset of LTE-derived metrics that show promise for further investigations. Future studies will likely benefit from larger sample size constructions so as to avoid the need for data filtering and feature selection techniques, which have the potential to significantly bias the machine learning procedures. (Source: Molecular Imaging and Biology)
Source: Molecular Imaging and Biology - January 25, 2023 Category: Molecular Biology Source Type: research
Development and Biodistribution of a Nerve Growth Factor Radioactive Conjugate for PET Imaging
ConclusionsWe conclude that conjugation of DFO to NGF through a thiourea linkage allows effective radiolabeling with zirconium-89 while maintaining NGF bioactivity. Following intravenous administration, the radiolabeled NGF targets non-neuronal tissues (e.g., kidney, liver), and although absolute brain uptake was very low, the brain uptake that was observed was restricted to the cortex. (Source: Molecular Imaging and Biology)
Source: Molecular Imaging and Biology - January 24, 2023 Category: Molecular Biology Source Type: research
MRI Contrasting Agent Based on Mn-MOF-74 Nanoparticles with Coordinatively Unsaturated Sites
ConclusionsThese results suggest that PEG-Mn-MOF-74 can be passively delivered to tumors and can act as a high-relaxivityT1 agent. (Source: Molecular Imaging and Biology)
Source: Molecular Imaging and Biology - January 18, 2023 Category: Molecular Biology Source Type: research
World Molecular Imaging Congress 2022
(Source: Molecular Imaging and Biology)
Source: Molecular Imaging and Biology - January 17, 2023 Category: Molecular Biology Source Type: research
Preclinical Identification Of Tumor-Draining Lymph Nodes Using a Multimodal Non-invasive In vivo Imaging Approach
ConclusionsWhen taken together, our results demonstrate the feasibility of18F-FDG-PET/MRI as a valid method for non-invasivein vivo, intraoperative, andex vivo identification of the lymphatic drainage and glucose metabolism within the TDLNs. In addition, using Patent Blue V provides additive value for the macroscopic localization of the lymphatic drainage both visually and byex vivo optical imaging analysis. Thus, both methods are valuable, easy to implement, and cost-effective for preclinical identification of the TDLN. (Source: Molecular Imaging and Biology)
Source: Molecular Imaging and Biology - January 4, 2023 Category: Molecular Biology Source Type: research
Data-Driven Identification of Targets for Fluorescence-Guided Surgery in Non-Small Cell Lung Cancer
ConclusionsEpCAM, CAIX, and Collagen XVII were identified using concomitant use of data-driven selection software and clinical evidence as promising targets for intraoperative fluorescence imaging for both major subtypes of non-small cell lung carcinomas. (Source: Molecular Imaging and Biology)
Source: Molecular Imaging and Biology - December 27, 2022 Category: Molecular Biology Source Type: research
Imaging Non-alcoholic Fatty Liver Disease Model Using H-1 and F-19 MRI
ConclusionsWe demonstrated longitudinal quantification of multiple MRI biomarkers of disease in NAFLD mice. The changes in liver PFC uptake in HFD mice were compared with healthy mice that suggests that19F MRI may be a viable biomarker of liver pathology. (Source: Molecular Imaging and Biology)
Source: Molecular Imaging and Biology - December 27, 2022 Category: Molecular Biology Source Type: research
Measurement of Protein Synthesis Rate in Rat by [11C]Leucine PET Imaging: Application to the TgF344-AD Model of Alzheimer ’s Disease
This study aimed to implement a method to measure the cerebral protein synthesis rate (PSR) with [11C]leucine PETin vivo in rats and evaluate potential PSR alterations longitudinally (6, 12 and 18 months old) in the TgF344-AD rat model of AD. Wistar, wild-type (WT) and TgF344-AD rats (TG) were scanned for 60 min with [11C]leucine. Arterial blood activity was monitored online and with discrete whole blood and plasma samples by γ-counting in Wistar rats, WT (n = 4) and TG (n = 5). Unlabelled amino acids were measured in plasma. The sensitivity of [11C]leucine PET to measure alterations in PSR was assessed in Wi...
Source: Molecular Imaging and Biology - December 20, 2022 Category: Molecular Biology Source Type: research
Sodium Multivitamin Transporter-Targeted Fluorochrome Facilitates Enhanced Metabolic Evaluation of Tumors Through Coenzyme-R Dependent Intracellular Signaling Pathways
ConclusionThese findings suggest that a SMVT-targeted NIR contrast agent can be a suitable tracer for imaging a wide range of malignancies as well as evaluating metabolic response to systemic therapies, similar to PET imaging with immune checkpoint inhibitors. (Source: Molecular Imaging and Biology)
Source: Molecular Imaging and Biology - December 19, 2022 Category: Molecular Biology Source Type: research
