Intricacies in the Preparation of Patient Doses of [177Lu]Lu-Rituximab and [177Lu]Lu-Trastuzumab Using Low Specific Activity [177Lu]LuCl3: Methodological Aspects
AbstractThe development of humanized monoclonal antibodies (MAbs) with Lutetium-177 ([177Lu]Lu3+) has brought a paradigm shift in the arena of targeted therapy of various cancers. [177Lu]Lu-DOTA-Rituximab and [177Lu]Lu-DOTA-Trastuzumab have gained prominence due to their improved therapeutic efficacy in the treatment of lymphoma and breast cancer. The clinical dose formulation of these radiolabeled MAbs, using low specific activity [177Lu]LuCl3, requires extensive optimization of the radiolabeling protocol. The present study merits the development of a single protocol which has been optimized for conjugation of Rituximab a...
Source: Molecular Imaging and Biology - September 6, 2023 Category: Molecular Biology Source Type: research
The Balance Between the Therapeutic Efficacy and Safety of [177Lu]Lu-NeoB in a Preclinical Prostate Cancer Model
ConclusionsTreatment with [177Lu]Lu-NeoB is effective in a preclinical PCa model. Adjusting the administered dose could positively impact the risk-benefit balance as a higher dose might not lead to an increased therapeutic effect, but it may lead to an increase in toxicological effects in healthy organs such as the kidneys. (Source: Molecular Imaging and Biology)
Source: Molecular Imaging and Biology - August 28, 2023 Category: Molecular Biology Source Type: research
In Vivo Electron Paramagnetic Resonance Molecular Profiling of Tumor Microenvironment upon Tumor Progression to Malignancy in an Animal Model of Breast Cancer
ConclusionMolecular TME profiling informs on alteration of tumor redox and metabolism during tumor staging. Early elevation of interstitial Pi at S1 may reflect tumor metabolic alterations that demand elevated phosphorus supply in accordance with the high rate growth hypothesis. These metabolic changes are supported by the following decrease of pHe due to a high tumor reliance on glycolysis and increase of intracellular GSH, a major intracellular redox buffer. The appreciable decrease in TMEpO2 was observed only at malignant S4, apparently as a consequence of tumor mass growth and corresponding decrease in perfusion effica...
Source: Molecular Imaging and Biology - August 23, 2023 Category: Molecular Biology Source Type: research
Optimization of an Allysine-Targeted PET Probe for Quantifying Fibrogenesis in a Mouse Model of Pulmonary Fibrosis
ConclusionA series of allysine-reactive PET probes with variations in the aldehyde-reactive moiety were evaluated in a pre-clinical model of lung fibrosis. The hydrazine-bearing probe,68GaPIF-7, exhibited the highest uptake in fibrogenic lung, low uptake in surrounding liver or heart tissue, and low lung uptake in healthy mice and should be considered for further clinical translation. (Source: Molecular Imaging and Biology)
Source: Molecular Imaging and Biology - August 23, 2023 Category: Molecular Biology Source Type: research
Correction: Detection of Endometriosis Lesions Using Gd-Based Collagen I Targeting Probe in Murine Models of Endometriosis
(Source: Molecular Imaging and Biology)
Source: Molecular Imaging and Biology - August 17, 2023 Category: Molecular Biology Source Type: research
Preliminary Evaluation of 18F-Labeled Benzylguanidine Analogs as NET Tracers for Myocardial Infarction Diagnosis
ConclusionsWith an obvious cardiac uptake contrast between normal mice and the myocardial infarction mouse model, [18F]3 appears to be a potential tool in the diagnosis of myocardial infarction. Therefore, it is necessary to conduct further structural modification studies on the chemical structure of [18F]3 to improve itsin vivo stability and diagnostic detection ability to achieve reliable and practical imaging effects. (Source: Molecular Imaging and Biology)
Source: Molecular Imaging and Biology - August 14, 2023 Category: Molecular Biology Source Type: research
A Novel Near-Infrared Fluorescence Probe THK-565 Enables In Vivo Detection of Amyloid Deposits in Alzheimer ’s Disease Mouse Model
ConclusionsA novel NIRF probe, THK-565, enabled thein vivo detection of A β deposits in the brains of the AD mouse model, suggesting that NIRF imaging with THK-565 could non-invasively assess disease-specific pathology in AD. (Source: Molecular Imaging and Biology)
Source: Molecular Imaging and Biology - August 14, 2023 Category: Molecular Biology Source Type: research
Deep Learning Radiomics Nomogram Based on Multiphase Computed Tomography for Predicting Axillary Lymph Node Metastasis in Breast Cancer
ConclusionsThe DLRN had good predictive value for ALNM in breast cancer patients and provide valuable information for individual treatment. (Source: Molecular Imaging and Biology)
Source: Molecular Imaging and Biology - August 10, 2023 Category: Molecular Biology Source Type: research
2nd Window NIR Imaging of Radiation Injury Mitigation Provided by Reduced Notch-Dll4 Expression on Vasculature
ConclusionsTogether, these data suggest Dll4 plays a key role in pathogenesis of radiation-induced vascular injury to the lung and kidney. (Source: Molecular Imaging and Biology)
Source: Molecular Imaging and Biology - August 2, 2023 Category: Molecular Biology Source Type: research
Deuterium Magnetic Resonance Spectroscopy Quantifies Tumor Fraction in a Mouse Model of a Mixed Radiation Necrosis / GL261-Glioblastoma Lesion
ConclusionsThis preclinical study supports the proposal that2H MR could occupy a well-defined secondary role when standard-of-care1H imaging is non-diagnostic regarding tumor presence and/or response to therapy. (Source: Molecular Imaging and Biology)
Source: Molecular Imaging and Biology - July 29, 2023 Category: Molecular Biology Source Type: research
PI-RADS-Based Segmented Threshold of PSMA-PET SUVmax Is Better than Traditional Fixed Threshold for Diagnosing Clinically Significant Prostate Cancer Especially for PI-RADS 3 Lesions
ConclusionsFor diagnosing csPCa, PI-RADS-based PSMA-PET segmented threshold achieved better performance than traditional fixed threshold, especially for PI-RADS 3 lesions. Multimodality diagnostic criteria demonstrated higher diagnostic performance than segmented threshold and significantly better than PSMA-PET fixed threshold for detecting csPCa. (Source: Molecular Imaging and Biology)
Source: Molecular Imaging and Biology - July 25, 2023 Category: Molecular Biology Source Type: research
Neurotoxin-Derived Optical Probes for Biological and Medical Imaging
This article presents state-of-the-art optical probes derived from neurotoxins targeting ion channels, with discussions of their applications in basic and translational biomedical research. It describes the design and production of probes and reviews their applications with advantages and limitations, with prospects for future improvements. Given the advances in imaging tools and expanding research areas benefiting from the use of optical probes, described here resources should assist the discovery process and facilitate high-precision interrogation and therapeutic interventions. (Source: Molecular Imaging and Biology)
Source: Molecular Imaging and Biology - July 19, 2023 Category: Molecular Biology Source Type: research
Pharmacological Characterization of [18F]-FNM and Evaluation of NMDA Receptors Activation in a Rat Brain Injury Model
ConclusionsIn spite of FNM ’s poor affinity for NMDAR PCP site, this study supports the ability of this tracer to track massive activation of NMDARs in neurological diseases. (Source: Molecular Imaging and Biology)
Source: Molecular Imaging and Biology - July 11, 2023 Category: Molecular Biology Source Type: research
Effects of Escitalopram on the Functional Neural Circuits in an Animal Model of Adolescent Depression
ConclusionThe administration of escitalopram had no therapeutic effect on the adolescent depression. (Source: Molecular Imaging and Biology)
Source: Molecular Imaging and Biology - July 11, 2023 Category: Molecular Biology Source Type: research
2-deoxy-2-[18F]fluoro-D-glucose Positron Emission Tomography to Monitor Lung Inflammation and Therapeutic Response to Dexamethasone in a Murine Model of Acute Lung Injury
Conclusions[18F]FDG PET noninvasively detects lung inflammation in ALI and its response to anti-inflammatory therapy in a preclinical model. However, high [18F]FDG uptake by bone, brown fat, and myocardium remains a technical limitation for quantification of [18F]FDG in the lungs. (Source: Molecular Imaging and Biology)
Source: Molecular Imaging and Biology - July 11, 2023 Category: Molecular Biology Source Type: research
