Associations between HLA-Cw1 and Systemic Treatment Response of Asian Psoriasis Patients
ConclusionThere is an association between HLA-Cw1 and treatment response to biologics, but not to non-biologics, in our Asian population of patients with moderate to severe psoriasis; however, the exact mechanism and role of HLA-Cw1 remain to be investigated. (Source: Molecular Diagnosis and Therapy)
Source: Molecular Diagnosis and Therapy - July 5, 2022 Category: Molecular Biology Source Type: research
Advances in RNA Viral Vector Technology to Reprogram Somatic Cells: The Paramyxovirus Wave
AbstractEthical issues are a significant barrier to the use of embryonic stem cells in patients due to their origin: human embryos. To further the development of stem cells in a patient application, alternative sources of cells were sought. A process referred to as reprogramming was established to create induced pluripotent stem cells from somatic cells, resolving the ethical issues, and vectors were developed to deliver the reprogramming factors to generate induced pluripotent stem cells. Early viral vectors used integrating retroviruses and lentiviruses as delivery vehicles for the transcription factors required to initi...
Source: Molecular Diagnosis and Therapy - June 28, 2022 Category: Molecular Biology Source Type: research
Modern Biomarkers for Autism Spectrum Disorder: Future Directions
AbstractAutism spectrum disorder is an increasingly prevalent neurodevelopmental disorder in the world today, with an estimated 2% of the population being affected in the USA. A major complicating factor in diagnosing, treating, and understanding autism spectrum disorder is that defining the disorder is solely based on the observation of behavior. Thus, recent research has focused on identifying specific biological abnormalities in autism spectrum disorder that can provide clues to diagnosis and treatment. Biomarkers are an objective way to identify and measure biological abnormalities for diagnostic purposes as well as to...
Source: Molecular Diagnosis and Therapy - June 27, 2022 Category: Molecular Biology Source Type: research
Immune-Checkpoint Expression on CD4, CD8 and NK Cells in Blood, Bronchoalveolar Lavage and Lymph Nodes of Sarcoidosis
ConclusionHigh expression of PD1 and TIGIT on T cells in BAL, as well as CTLA-4 and TIGIT on T cells in LLN, suggest that inhibition of these molecules could be a therapeutic strategy for avoiding the development of chronic inflammation and tissue damage in sarcoidosis patients.Graphical Abstract (Source: Molecular Diagnosis and Therapy)
Source: Molecular Diagnosis and Therapy - June 27, 2022 Category: Molecular Biology Source Type: research
The lncRNA PRINS-miRNA-mRNA Axis Gene Expression Profile as a Circulating Biomarker Panel in Psoriasis
ConclusionsBased on our results, we suggest that restoring the alteredPRINS-miRNA-mRNA axis gene expression levels might represent a tool to prevent psoriasis worsening, along with standard therapy. Thus, on the clinical practice level, thePRINS-miRNA-mRNA axis expression profile can be utilized in designing specific targeted therapy aimed at applying a personalized medicine approach among patients with psoriasis. (Source: Molecular Diagnosis and Therapy)
Source: Molecular Diagnosis and Therapy - June 27, 2022 Category: Molecular Biology Source Type: research
Effect of E-cadherin on Prognosis of Colorectal Cancer: A Meta-Analysis Update
ConclusionAlthough not supported by GEPIA, our meta-analysis provided abundant data to suggest that low expression of E-cadherin is associated with poor prognosis in colorectal cancer patients and is an important factor influencing adverse clinicopathological features. Therefore, E-cadherin may be used to predict the prognosis of colorectal cancer and provide guidance for clinical treatment. (Source: Molecular Diagnosis and Therapy)
Source: Molecular Diagnosis and Therapy - June 22, 2022 Category: Molecular Biology Source Type: research
Optimization of a Quantitative PCR Methodology for Detection of Aspergillus spp. and Rhizopus arrhizus
DiscussionThis work provides evidence for the use of a qPCR multiplex method based on SYBR Green and melting curve analysis of PCR products for the detection ofA. fumigatus,A. flavus,A. niger,A. terreus, andR. arrhizus. The proposed method is simpler and less expensive than available kits based on fluorescent probes and can be used for aiding diagnosis of the most relevant invasive filamentous fungi, particularly in low-income health care institutions. (Source: Molecular Diagnosis and Therapy)
Source: Molecular Diagnosis and Therapy - June 16, 2022 Category: Molecular Biology Source Type: research
Biomarker Determinants of Early Anthracycline-Induced Left Ventricular Dysfunction in Breast Cancer: A Systematic Review and Meta-Analysis
ConclusionsThis is the first meta-analysis quantifying the association of biomarkers and early ACIC presentation in the breast cancer population. This may be of clinical relevance in the timely identification of patients at high risk of ACIC, allowing for closer monitoring and chemotherapy adjustments. (Source: Molecular Diagnosis and Therapy)
Source: Molecular Diagnosis and Therapy - June 16, 2022 Category: Molecular Biology Source Type: research
Performance Evaluation of Three DNA Sample Tracking Tools in a Whole Exome Sequencing Workflow
ConclusionOur conclusion is that the Human Sample ID kit from pxlence is the most cost effective of the three tested tools for DNA sample tracking and identification. (Source: Molecular Diagnosis and Therapy)
Source: Molecular Diagnosis and Therapy - May 28, 2022 Category: Molecular Biology Source Type: research
Selective Signal Capture from Multidimensional GPCR Outputs with Biased Agonists: Progress Towards Novel Drug Development
AbstractG protein coupled receptors (GPCRs) are a superfamily of transmembrane-spanning receptors that are activated by multiple endogenous ligands and are the most common target for agonist or antagonist therapeutics across a broad spectrum of diseases. Initial characterization within the superfamily suggested that a receptor activated a single intracellular pathway, depending on the G protein to which it coupled. However, it has become apparent that a given receptor can activate multiple different pathways, some being therapeutically desirable, while others are neutral or promote deleterious signaling. The activation of ...
Source: Molecular Diagnosis and Therapy - May 20, 2022 Category: Molecular Biology Source Type: research
Gestational Diabetes is Associated with an Increased Expression of miR-27a in Peripheral Blood Mononuclear Cells
ConclusionsThe results obtained illustrate the potential of PBMC-derived microRNA miR-27a-3p to serve as a diagnostic biomarker of GDM. On the other hand, MiR-27a and miR-340 may help in assessing the metabolic status relevant for pregnancy. (Source: Molecular Diagnosis and Therapy)
Source: Molecular Diagnosis and Therapy - May 16, 2022 Category: Molecular Biology Source Type: research
Lutetium Lu 177 Vipivotide Tetraxetan: First Approval
This article summarizes the milestones in the development of Lutetium Lu 177 vipivotide tetraxetan leading to this first approval as a therapeutic radioligand for mCRPC. (Source: Molecular Diagnosis and Therapy)
Source: Molecular Diagnosis and Therapy - May 13, 2022 Category: Molecular Biology Source Type: research
Targeting Genetic Modifiers of HBG Gene Expression in Sickle Cell Disease: The miRNA Option
AbstractSickle cell disease (SCD) is one of the most common inherited hemoglobinopathy disorders that affects millions of people worldwide. Reactivation of HBG (HBG1, HBG2) gene expression and induction of fetal hemoglobin (HbF) is an important therapeutic strategy for ameliorating the clinical symptoms and severity of SCD. Hydroxyurea is the only US FDA-approved drug with proven efficacy to induce HbF in SCD patients, yet serious complications have been associated with its use. Over the last three decades, numerous additional pharmacological agents that reactivate HBG transcription in vitro have been investigated, but few...
Source: Molecular Diagnosis and Therapy - May 12, 2022 Category: Molecular Biology Source Type: research
Ongoing Challenges in the Diagnosis of 11p15.5-Associated Imprinting Disorders
AbstractThe overgrowth disorder Beckwith –Wiedemann syndrome and the growth restriction disorder Silver–Russell syndrome have been described as ‘mirror’ syndromes, in both their clinical features and molecular causes. Clinically, their nonspecific features, focused around continuous variables of atypical growth, make it hard to set diagnostic thresholds that are pragmatic without potentially excluding some cases. Molecularly, both are imprinting disorders, classically associated with ‘opposite’ genetic and epigenetic changes to genes on chromosome 11p15, but both are associated with somatic mosaicism as well as...
Source: Molecular Diagnosis and Therapy - May 6, 2022 Category: Molecular Biology Source Type: research
A Window of Opportunity for Newborn Screening
AbstractMolecular diagnostics and therapies play a central role in an era of precision medicine, with the promise of more accurate diagnoses and more effective treatments. Universal newborn screening (NBS) identifies those health conditions that must be treated in early life and before clinical symptoms become apparent, to maximize effectiveness, prevent morbidity, and reduce or eliminate mortality. However, enthusiasm about NBS as the logical platform for early identification is tempered by the realization that NBS under public health authority exists in a complex ecology in which technology and medicine intersect with po...
Source: Molecular Diagnosis and Therapy - May 4, 2022 Category: Molecular Biology Source Type: research
