The Role οf Ion Channels in the Development and Progression of Prostate Cancer
AbstractIon channels have major regulatory functions in living cells. Apart from their role in ion transport, they are responsible for cellular electrogenesis and excitability, and may also regulate tissue homeostasis. Although cancer is not officially classified as a channelopathy, it has been increasingly recognized that ion channel aberrations play an important role in virtually all cancer types. Ion channels can exert pro-tumorigenic activities due to genetic or epigenetic alterations, or as a response to molecular signals, such as growth factors, hormones, etc. Increasing evidence suggests that ion channels and pumps ...
Source: Molecular Diagnosis and Therapy - January 4, 2023 Category: Molecular Biology Source Type: research
Emerging Landscape of SARS-CoV-2 Variants and Detection Technologies
AbstractIn 2019, a new coronavirus was identified that has caused significant morbidity and mortality worldwide. Like all RNA viruses, severe acute respiratory syndrome coronavirus 2 (SARS-Cov-2) evolves over time through random mutation resulting in genetic variations in the population. Although the currently approved coronavirus disease 2019 vaccines can be given to those over 5 years of age and older in most countries, strikingly, the number of people diagnos ed positive for SARS-Cov-2 is still increasing. Therefore, to prevent and control this epidemic, early diagnosis of infected individuals is of great importance....
Source: Molecular Diagnosis and Therapy - December 28, 2022 Category: Molecular Biology Source Type: research
Introducing Circulating Vasculature-Related Transcripts as Biomarkers in Coronary Artery Disease
ConclusionThe seven circulating vascular-related RNA transcripts under study could serve as potential CAD biomarkers, reflecting the cellular vascular function, proliferation, and inflammatory processes in CAD patients. Therefore, blood transcriptome analysis opens new frontiers for the non-invasive diagnosis of CAD. (Source: Molecular Diagnosis and Therapy)
Source: Molecular Diagnosis and Therapy - December 20, 2022 Category: Molecular Biology Source Type: research
Candidate MicroRNA Biomarkers in Lupus Nephritis: A Meta-analysis of Profiling Studies in Kidney, Blood and Urine Samples
ConclusionUsing a meta-analytical approach, our study proposes three meta-miRNA panels that could be the target of further research to assess their potential as therapeutic targets/biomarkers in LN disease. (Source: Molecular Diagnosis and Therapy)
Source: Molecular Diagnosis and Therapy - December 15, 2022 Category: Molecular Biology Source Type: research
Gene Therapy for Cardiomyocyte Renewal: Cell Cycle, a Potential Therapeutic Target
AbstractHeart disease is the primary cause of death worldwide. Even though extensive research has been done, and many pharmacological and surgical treatments have been introduced to treat heart disease, the mortality rate still remains high. Gene therapy is widely used to understand molecular mechanisms of myocardial infarction and to treat cardiomyocyte loss. It was reported that adult cardiomyocytes proliferate at a very low rate; thus, targeting their proliferation has become a new regenerative therapeutic approach. Currently, re-activating cardiomyocyte proliferation appears to be one of the most promising methods to p...
Source: Molecular Diagnosis and Therapy - December 13, 2022 Category: Molecular Biology Source Type: research
Acknowledgement to Referees
(Source: Molecular Diagnosis and Therapy)
Source: Molecular Diagnosis and Therapy - December 12, 2022 Category: Molecular Biology Source Type: research
Systematic In-Silico Evaluation of the Diagnostic Impact of Mpox Genome Variants in the Current Outbreak
ConclusionsOur analysis shows the importance of continuous monitoring of mpox virus detection primer efficacy and provides the list of oligos with potentially reduced detection efficiency in the current mpox virus outbreak. (Source: Molecular Diagnosis and Therapy)
Source: Molecular Diagnosis and Therapy - December 10, 2022 Category: Molecular Biology Source Type: research
Pitfalls in Cutaneous Melanoma Diagnosis and the Need for New Reliable Markers
AbstractCutaneous melanoma is one of the most aggressive forms of skin cancer, with the development of advanced stage disease resulting in a high rate of patient mortality. Accurate diagnosis of melanoma at an early stage is essential to improve patient outcomes, as this enables treatment before the cancer has metastasised. Histopathologic analysis is the current gold standard for melanoma diagnosis, but this can be subjective due to discordance in interpreting the morphological heterogeneity in melanoma and other skin lesions. Immunohistochemistry (IHC) is sometimes employed as an adjunct to conventional histology, but it...
Source: Molecular Diagnosis and Therapy - December 7, 2022 Category: Molecular Biology Source Type: research
Profiling of Circulating Gene Expression Reveals Molecular Signatures Associated with Intracranial Aneurysm Rupture Risk
ConclusionsHere, we identified genes associated with IA risk, as quantified by common clinical metrics. Preliminary classification models demonstrated feasibility of assessing IA risk using whole blood expression. (Source: Molecular Diagnosis and Therapy)
Source: Molecular Diagnosis and Therapy - December 3, 2022 Category: Molecular Biology Source Type: research
Detection of the Copy Number Variants of Genes in Patients with Familial Cardiac Diseases by Massively Parallel Sequencing
ConclusionThe confirmed CNVs present in all cases BF values> 60, thus establishing a threshold to consider real CNVs in the calling process carried out by ExomeDepth on our gene panel. (Source: Molecular Diagnosis and Therapy)
Source: Molecular Diagnosis and Therapy - December 1, 2022 Category: Molecular Biology Source Type: research
Genetic Alterations of Melanoma Brain Metastases: A Systematic Review and Meta-Analysis
ConclusionMBMs harbored actionable molecular alterations that could be exploited as therapeutic targets to improve the poor prognosis of patients. (Source: Molecular Diagnosis and Therapy)
Source: Molecular Diagnosis and Therapy - November 19, 2022 Category: Molecular Biology Source Type: research
Genetic Profiling of Diffuse Large B-Cell Lymphoma: A Comparison Between Double-Expressor Lymphoma and Non-Double-Expressor Lymphoma
ConclusionThere was difference in profiling of altered genes and signaling pathways between the DEL group and the non-DEL group. The presence of DEL alone should not be considered as an adverse prognostic indicator, andBCL2 alteration could define a subset of patients with poor prognosis within DEL. (Source: Molecular Diagnosis and Therapy)
Source: Molecular Diagnosis and Therapy - November 18, 2022 Category: Molecular Biology Source Type: research
Exploratory Prognostic Biomarkers of Complement-Mediated Thrombotic Microangiopathy (CM-TMA) in Adults with Atypical Hemolytic Uremic Syndrome (aHUS): Analysis of a Phase III Study of Ravulizumab
ConclusionThis analysis identified a set of biomarkers that may show utility in the prognosis of CM-TMA, including their potential for measuring and predicting response to anti-C5 therapy. Further studies are required to enhance patient risk stratification and improve management of these vulnerable patients.Clinical Trials RegistrationNCT02949128, ClinicalTrials.gov. (Source: Molecular Diagnosis and Therapy)
Source: Molecular Diagnosis and Therapy - November 4, 2022 Category: Molecular Biology Source Type: research
Role of Long Intergenic Noncoding RNAs in Cancers with an Overview of MicroRNA Binding
AbstractLong intergenic noncoding RNAs are transcripts originating from the regions without annotated coding genes. They are located mainly in the nucleus and regulate gene expression. Long intergenic noncoding RNAs can be also found in the cytoplasm acting as molecular sponges of certain microRNAs. This is crucial in various biological and signaling pathways. Expression levels of many long intergenic noncoding RNAs are disease related. In this article, we focus on the long intergenic noncoding RNAs and their relation to different types of cancer. Studies showed that abnormal expression of long intergenic noncoding RNA der...
Source: Molecular Diagnosis and Therapy - October 26, 2022 Category: Molecular Biology Source Type: research
Epidermolysis Bullosa: A Review of the Tissue-Engineered Skin Substitutes Used to Treat Wounds
AbstractSkin wound healing is a crucial process for regenerating healthy skin and avoiding the undesired consequences associated with open skin wounds. For epidermolysis bullosa (EB), a debilitating group of fragile skin disorders currently without a cure, skin blistering can often be severe and heal poorly, increasing susceptibility to life-threatening complications. To prevent these, investigational therapies have been exploring the use of tissue-engineered skin substit utes (TESSs) aimed at replacing damaged skin and promoting long-term wound closure. These products have either been developed in house or commercially...
Source: Molecular Diagnosis and Therapy - October 17, 2022 Category: Molecular Biology Source Type: research
