Thymine DNA glycosylase regulates cell-cycle-driven p53 transcriptional control in pluripotent cells
Beyond its canonical function of regulating cell numbers in an organism, the cell cycle is proposed to work as a mechanism to temporally separate multiple cell fate choices. Aranda et al. uncover the role of TDG in regulating the cell-cycle-based segregation of the differentiation potential of pluripotent cells. (Source: Molecular Cell)
Source: Molecular Cell - July 27, 2023 Category: Cytology Authors: Sergi Aranda, Anna Alcaine-Colet, Cecilia Ballar é, Enrique Blanco, Ivano Mocavini, Aleksandra Sparavier, Pedro Vizán, Eva Borràs, Eduard Sabidó, Luciano Di Croce Tags: Article Source Type: research
FUBP1 is a general splicing factor facilitating 3 ′ splice site recognition and splicing of long introns
Ebersberger et al. identify the RNA-binding protein FUBP1 as a key splicing factor that binds to a hitherto unknown cis-regulatory motif at 3′ splice sites. Multivalent interactions of FUBP1 with splice site components support spliceosome assembly at multiple stages and ensure efficient splicing of long intron s. (Source: Molecular Cell)
Source: Molecular Cell - July 27, 2023 Category: Cytology Authors: Stefanie Ebersberger, Clara Hipp, Miriam M. Mulorz, Andreas Buchbender, Dalmira Hubrich, Hyun-Seo Kang, Santiago Mart ínez-Lumbreras, Panajot Kristofori, F.X. Reymond Sutandy, Lidia Llacsahuanga Allcca, Jonas Schönfeld, Cem Bakisoglu, Anke Busch, Heike Tags: Article Source Type: research
Visualization of direct and diffusion-assisted RAD51 nucleation by full-length human BRCA2 protein
Belan et al. exploit single-molecule imaging to reveal the mechanism of early steps of presynaptic RAD51 filament establishment by full-length human BRCA2 protein. BRCA2 nucleates and stabilizes RAD51 on ssDNA and ds-ssDNA junctions, either directly or through a diffusion-assisted delivery mechanism involv ing BRCA2 sliding on dsDNA. (Source: Molecular Cell)
Source: Molecular Cell - July 26, 2023 Category: Cytology Authors: Ondrej Belan, Luke Greenhough, Lucas Kuhlen, Roopesh Anand, Artur Kaczmarczyk, Dominika T. Gruszka, Hasan Yardimci, Xiaodong Zhang, David S. Rueda, Stephen C. West, Simon J. Boulton Tags: Article Source Type: research
The solute carrier SPNS2 recruits PI(4,5)P2 to synergistically regulate transport of sphingosine-1-phosphate
Tang et al. use native mass spectrometry to show that the lysolipid transporter SPNS2 binds preferentially to the phospholipid PI(4,5)P2. Using hydrogen-deuterium exchange to probe the dynamics of SPNS2, they show how PI(4,5)P2 and the signaling lipid S1P act together to promote conformational changes tha t facilitate substrate transport. (Source: Molecular Cell)
Source: Molecular Cell - July 26, 2023 Category: Cytology Authors: Haiping Tang, Huanyu Li, Dheeraj Prakaash, Conrado Pedebos, Xingyu Qiu, David B. Sauer, Syma Khalid, Katharina Duerr, Carol V. Robinson Tags: Article Source Type: research
Genome-scale mapping of DNA damage suppressors through phenotypic CRISPR-Cas9 screens
Zhao et al. performed phenotypic CRISPR screens to uncover genes and pathways that suppress spontaneous and DNA replication stress-associated DNA damage in human cells. Among the identified genes, GNB1L, which is deleted in the 22q11.2 syndrome, is essential for the biosynthesis of kinases of the ATM/ATR family. (Source: Molecular Cell)
Source: Molecular Cell - July 20, 2023 Category: Cytology Authors: Yichao Zhao, Daniel Tabet, Diana Rubio Contreras, Linjiang Lao, Arne Nedergaard Kousholt, Jochen Weile, Henrique Melo, Lisa Hoeg, Sumin Feng, Atina G. Cot é, Zhen-Yuan Lin, Dheva Setiaputra, Jos Jonkers, Anne-Claude Gingras, Fernando Gómez Herreros, Fre Tags: Resource Source Type: research
