Abstract PR14: Real-time in-vivo image-guided cell-cycle perturbation to increase tumor chemosensitivity
Tumor heterogeneity has been recognized as a major reason for anticancer drug resistance and therapy failure in solid cancers. Therefore, we simply categorized the tumor heterogeneity as cycling and quiescent cancer cells. A multi-color genetic reporter system has been previously-developed termed fluorescence ubiquitination cell cycle indicator (FUCCI) (Cell 2008; 132:487-98) whereby cycling cells fluorescence yellow/green and quiescent cells fluoresce red. We demonstrated the monitoring of real-time spatiotemporal cell cycle dynamics of cancer cells throughout a live tumor intravitally using a FUCCI system. According to c...
Source: Molecular Cancer Research - November 9, 2016 Category: Cancer & Oncology Authors: Yano, S., Tazawa, H., Kishimoto, H., Kagawa, S., Kadowaki, Y., Ishido, N., Okamoto, T., Urata, Y., Takehara, K., Hoffman, R. M., Fujiwara, T. Tags: Getting out of Cycle: G0 and Senescence: Oral Presentations - Proffered Abstracts Source Type: research
Abstract B14: Feedback regulation between atypical E2Fs and APC/CCdh1 coordinates cell cycle progression.
In conclusion, we uncovered a feedback loop between atypical E2Fs and APC/CCdh1, which ensures balanced expression of cell cycle genes and normal cell cycle progression.Citation Format: Michiel Boekhout, Ruixue Yuan, Annelotte P. Wondergem, Hendrika A. Segeren, Elsbeth A. van Liere, Nesibu Awol, Imke Jansen, Rob MF Wolthuis, Alain de Bruin, Bart Westendorp. Feedback regulation between atypical E2Fs and APC/CCdh1 coordinates cell cycle progression.. [abstract]. In: Proceedings of the AACR Precision Medicine Series: Cancer Cell Cycle - Tumor Progression and Therapeutic Response; Feb 28-Mar 2, 2016; Orlando, FL. Philadelphia ...
Source: Molecular Cancer Research - November 9, 2016 Category: Cancer & Oncology Authors: Boekhout, M., Yuan, R., Wondergem, A. P., Segeren, H. A., Liere, E. A. v., Awol, N., Jansen, I., Wolthuis, R. M., Bruin, A. d., Westendorp, B. Tags: E2F Family Functions: Alterations and Consequences: Poster Presentations - Proffered Abstracts Source Type: research
Abstract PR13: Germ-line mutations in CDC20 result in familial cancers via deregulation of the cell cycle
Conclusions: Our preliminary data suggest that L151R and N331K may drive cancer through the insensitivity of Cdc20 to the SAC. To our knowledge this is the first description of CDC20 as a cancer predisposition gene. The rapid division of cancer cells demands that they maintain high APC activity and this may represent an Achilles heel that can be exploited therapeutically.This abstract is also being presented as Poster B25.Citation Format: Ester Castellsague, Jian Carrot-Zhang, Isabelle Gamache, Barbara Rivera, Mohamed Moustafa, David Barford, Jacek Majewski, Jose Teodoro, William David Foulkes. Germ-line mutations in CDC20...
Source: Molecular Cancer Research - November 9, 2016 Category: Cancer & Oncology Authors: Castellsague, E., Carrot-Zhang, J., Gamache, I., Rivera, B., Moustafa, M., Barford, D., Majewski, J., Teodoro, J., Foulkes, W. D. Tags: Other Topics: Oral Presentations - Proffered Abstracts Source Type: research
Abstract A13: A new mitochondrial pool of cyclin E, regulated by Drp1, is linked to cell-density-dependent cell proliferation
Cyclin E is indispensable for release from quiescence. However, the regulation and function of the crucial cell cycle regulator cyclin E (CycE) remains elusive. Unlike other cyclins, CycE can be uniquely controlled by mitochondrial energetics, the exact mechanism being unclear. Using mammalian cells (in vitro) and Drosophila (in vivo) model systems in parallel, we show that CycE can be directly regulated by mitochondria through its recruitment to the organelle. Active mitochondrial bioenergetics maintains a distinct mitochondrial pool of CycE (mtCycE) lacking a key phosphorylation required for its degradation. Loss of the ...
Source: Molecular Cancer Research - November 9, 2016 Category: Cancer & Oncology Authors: Parker, D., Mitra, K. Tags: Targeting CDK/cyclins: Hormone Dependent Cancers and Beyond: Poster Presentations - Proffered Abstracts Source Type: research
Abstract IA12: Targeting the cell cycle in pediatric solid tumors
My lab is interested in understanding how proliferation and differentiation are coordinated during development and how those processes become uncoupled in pediatric solid tumors. We are also interested in identifying tumor vulnerabilities that can be exploited therapeutically. We have developed a unique resource for studying the developmental origins of childhood solid tumors and this resource is shared freely with no obligation to collaborate through the Childhood Solid Tumor Network (www.stjude.org/cstn). We have also developed a rigorous and comprehensive preclinical phase I/II/III testing program (Langenau et al. Cance...
Source: Molecular Cancer Research - November 9, 2016 Category: Cancer & Oncology Authors: Dyer, M. Tags: Rb Bench to Bedside: Novel Functions and Clinical Implications: Oral Presentations - Invited Abstracts Source Type: research
