MiR-202-5p attenuates neurological deficits and neuronal injury in MCAO model rats and OGD-induced injury in Neuro‐2a cells by targeting eIF4E-mediated induction of autophagy and inhibition of Akt/GSK-3β pathway
Publication date: Available online 24 December 2019Source: Molecular and Cellular ProbesAuthor(s): Bing Li, Zhi Huang, Ju Meng, Wenfeng Yu, Hua YangAbstractIschemic stroke is a common cerebrovascular disease caused by insufficient blood supply to the brain. In recent years, studies have demonstrated that microRNAs (miRNAs) are involved in a variety of biological processes in the nervous system. However, the effects of miR-202-5p on cerebral ischemic stroke injury have not been completely elucidated. In our study, N2a cells were subjected to oxygen-glucose deprivation/reoxygenation (OGD/R) treatment, and middle cerebral art...
Source: Molecular and Cellular Probes - December 24, 2019 Category: Molecular Biology Source Type: research
Origin recognition complex subunit 1 regulates cell growth and metastasis in glioma by altering activation of ERK and JNK signaling pathway
Publication date: Available online 20 December 2019Source: Molecular and Cellular ProbesAuthor(s): Wenmin Xiong, Chen Xie, Yang Qiu, Ziwei Tu, Qiaoying GongAbstractOrigin recognition complex subunit 1(ORC1) is reported to be closely associated with the cell cycle. However, studies on the role of ORC1 in glioma remain undefined. The aim of the present study was to determine whether ORC1 affects cell migration, invasion, apoptosis, and proliferation and to explore the possible underlying mechanism. GEO database analysis indicated that ORC1 was significantly upregulated in glioma, while Gene set enrichment analysis (GSEA) ana...
Source: Molecular and Cellular Probes - December 20, 2019 Category: Molecular Biology Source Type: research
Development of a real-time reverse transcription recombinase polymerase amplification assay for rapid detection of spring viremia of carp virus
In this study, the real-time reverse transcription recombinase polymerase amplification (RT-RPA) assay was developed and validated. Primers and probe targeting the conserved region of M gene were designed and applied to the real-time RT-RPA assay that performed at 39 °C for 20 min. The specificity analysis showed that no cross-reaction with other pathogenic viruses of fish was found, indicating appropriate specificity of the assay. In vitro transcribed RNA standards were used to estimate the sensitivity of the assay and the detection limit was 102copies/reaction. To further evaluate the assay, 65 clinical samples were...
Source: Molecular and Cellular Probes - December 19, 2019 Category: Molecular Biology Source Type: research
Epidemiological investigation of feline infectious peritonitis in cats living in Harbin, Northeast China from 2017 to 2019 using a combination of an EvaGreen-based real-time RT-PCR and serum chemistry assays
In this study, we isolated a wild-type FIPV HRB-17 epidemic strain from the blood sample of household pet cat exhibiting the characteristic wet-form FIP symptoms, which has been confirmed further by animal infection. Further, we developed an EvaGreen-based real-time RT-PCR assay for the accurate detection of FCoV based on the amplification of the highly conserved FIPV N gene. Then, using a combination of the real-time RT-PCR approach and a serum chemistry assay, we performed an epidemiological survey of FIPV infection in cats living in Harbin City, Northeast China. The results indicated that the EvaGreen-based real-time RT...
Source: Molecular and Cellular Probes - December 14, 2019 Category: Molecular Biology Source Type: research
Depletion of miR-380 mitigates human bronchial epithelial cells injury to improve chronic obstructive pulmonary disease through targeting CHRNA4
ConclusionsDepletion of miR-380 alleviated cells damage caused by CSE through targeting CHRNA4, suggesting that miR-380/CHRNA4 may serve as novel therapeutic targets for COPD treatment. (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - December 10, 2019 Category: Molecular Biology Source Type: research
Establishment of canine macrophages stably expressing GFP-tagged canine LC3 protein for effectively detecting autophagy
Publication date: Available online 7 December 2019Source: Molecular and Cellular ProbesAuthor(s): Dan Cui, Shanshan Huo, Xing Wang, Zhiqiang Zheng, Yonghong Zhang, Jianlou Zhang, Fei ZhongAbstractAutophagy plays a crucial role in eliminating protein aggregates, damaged organelles and invading pathogens. Genetically engineered cell line stably expressing green fluorescent protein (GFP)-tagged microtubule-associated protein light chain 3 (LC3) is extensively used to test autophagy through observing GFP puncta formation in the cells by fluorescence imaging. However, canine LC3 (cLC3) gene has not been cloned, therefore, GFP-t...
Source: Molecular and Cellular Probes - December 7, 2019 Category: Molecular Biology Source Type: research
HOXC10 promotes proliferation and attenuates lipid accumulation of sheep bone marrow mesenchymal stem cells
In this study, we investigated the effect of HOXC10 on proliferation and adipogenic differentiation in sheep bone marrow mesenchymal stem cells (sBMSCs). In sBMSCs, HOXC10 overexpression promoted cell proliferation and upregulated the expression of p-PI3K, p-AKT, p-p70S6K, p-MEK, and p-ERK, whereas HOXC10 knockdown was associated with the opposite effects. These results suggested that HOXC10 may promote cell proliferation by activating the MEK/ERK and PI3K/AKT/mTOR/p70S6K signaling pathways. In addition, we found that HOXC10 expression was negatively associated with lipid accumulation in adipogenic-differentiated sBMSCs. H...
Source: Molecular and Cellular Probes - December 7, 2019 Category: Molecular Biology Source Type: research
lncRNA PVT1 aggravates doxorubicin-induced cardiomyocyte apoptosis by targeting the miR-187-3p/AGO1 axis
ConclusionIn doxorubicin-induced cardiomyocyte toxicity, the highly expressed lncRNA PVT1 enhances the expression of AGO1 by sponge adsorption of miR-187-3p. Decreasing the expression of lncRNA PVT1 inhibits the adsorption of miR-187-3p through competing endogenous (ce) RNA, thereby reducing the expression of AGO1 and decreasing the apoptosis of cardiomyocytes. (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - November 28, 2019 Category: Molecular Biology Source Type: research
Development of an EvaGreen based real-time RT-PCR assay for rapid detection, quantitation and diagnosis of goose calicivirus
In this study, an EvaGreen based fluorescence quantitative real-time RT-PCR assay was developed and optimized for the detection of GCVs. The assay sensitively detected GCV RNA template with a good linear standard curve We also demonstrated the specificity and reproducibility of the detection method for GCVs. Thus, the method developed in this study will benefit the investigation of possible sporadic outbreaks of CV infections in geese, as well as epidemiological and etiological studies of GCVs. (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - November 18, 2019 Category: Molecular Biology Source Type: research
Verapamil and collagenase differentially affect collagen metabolism in experimental model of Peyronie's disease
ConclusionVER and COLL attenuate collagen biosynthesis in both fibroblast models. The VER-dependent inhibition of collagen biosynthesis was accompanied by inhibition of DNA biosynthesis at high prolidase activity, while COLL affected this process through inhibition of prolidase activity at high rate of DNA biosynthesis. It shows that anti-fibrotic activity of VER/COLL and anti-inflammatory activity of VER may represent approach to establish standard treatment of PD. (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - November 15, 2019 Category: Molecular Biology Source Type: research
Duplex TaqMan real-time PCR assay for simultaneous detection and quantification of Anaplasma capra and Anaplasma phagocytophilum infection
This study aimed to develop a rapid, sensitive and reliable duplex real-time PCR assay for the specific detection and differentiation of these Anaplasma species.We designed primers and probes against the conserved regions of A. capra groEL and A. phagocytophilum 16S rRNA genes. A range of PCR-related parameters were evaluated such as the dosage of primers and probes, and annealing temperature. The specificity, sensitivity and repeatability of this assay were evaluated. Assay performance was further evaluated using samples collected from 124 goats in four regions of Henan, China. This set of samples was also tested using co...
Source: Molecular and Cellular Probes - November 12, 2019 Category: Molecular Biology Source Type: research
Editorial Board
Publication date: December 2019Source: Molecular and Cellular Probes, Volume 48Author(s): (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - November 10, 2019 Category: Molecular Biology Source Type: research
Technical progress in circulating tumor DNA analysis using next generation sequencing
Publication date: Available online 8 November 2019Source: Molecular and Cellular ProbesAuthor(s): Yunfei Bai, Zexin Wang, Zhiyu Liu, Geyu Liang, Wanjun Gu, Qinyu GeAbstractCirculating tumor DNA (ctDNA) is tumor-derived, fragmented DNA that circulates freely in body fluids, predominantly in the peripheral blood. Recently, ctDNA analysis has been suggested as a complement to tissue biopsy in the detection and treatment of cancer. Genetic and epigenetic information specific to tumor cells, including single nucleotide variations, copy number variations, and modified methylation patterns, can be detected in ctDNA. Importantly, ...
Source: Molecular and Cellular Probes - November 9, 2019 Category: Molecular Biology Source Type: research
MiRNA-483–5p is involved in the pathogenesis of osteoporosis by promoting osteoclast differentiation
ConclusionsOur study suggests that miRNA-483–5p is involved in the pathogenesis of osteoporosis by promoting osteoclast differentiation. (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - November 6, 2019 Category: Molecular Biology Source Type: research
Circ_016719 plays a critical role in neuron cell apoptosis induced by I/R via targeting miR-29c/Map2k6
ConclusionsOur results suggest that circ_016719 directly targets miR-29c, and thereby regulates the expression and functions of Map2k6, which significantly contributes to the pro-apoptotic role of circ_016719. (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - November 6, 2019 Category: Molecular Biology Source Type: research
