Molecular and Biochemical Parasitology 
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This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.Structural and kinetic characterization of Trypanosoma congolense pyruvate kinase
Publication date: March 2020Source: Molecular and Biochemical Parasitology, Volume 236Author(s): Joar Esteban Pinto Torres, Meng Yuan, Julie Goossens, Wim Versées, Guy Caljon, Paul A. Michels, Malcolm D. Walkinshaw, Stefan Magez, Yann G.-J. Sterckx (Source: Molecular and Biochemical Parasitology)
Source: Molecular and Biochemical Parasitology - February 27, 2020 Category: Parasitology Source Type: research
Structural and kinetic characterisation of Trypanosoma congolense pyruvate kinase
Publication date: Available online 19 February 2020Source: Molecular and Biochemical ParasitologyAuthor(s): Joar Esteban Pinto Torres, Meng Yuan, Julie Goossens, Wim Versées, Guy Caljon, Paul A. Michels, Malcolm D. Walkinshaw, Stefan Magez, Yann G.-J. SterckxAbstractTrypanosoma are blood-borne parasites and are the causative agents of neglected tropical diseases (NTDs) affecting both humans and animals. These parasites mainly rely on glycolysis for their energy production within the mammalian host, which is why trypanosomal glycolytic enzymes have been pursued as interesting targets for the development of trypanocidal dru...
Source: Molecular and Biochemical Parasitology - February 19, 2020 Category: Parasitology Source Type: research
YihA GTPases localize to the apicoplast and mitochondrion of the malaria parasite and interact with LSU of organellar ribosomes
Publication date: Available online 11 February 2020Source: Molecular and Biochemical ParasitologyAuthor(s): Ankit Gupta, Kirti Gupta, Saman HabibAbstractThe YihA TRAFAC GTPases are critical for late-stage assembly of the ribosomal large subunit (LSU). In order to explore biogenesis of the reduced organellar ribosomes of the malaria parasite, we identified three nuclear-encoded homologs of YihA in Plasmodium falciparum. PfYihA1 targeted to the parasite apicoplast, PfYihA2 to the mitochondrion, and PfYihA3 was found in both the apicoplast and cytosol. The three PfYihA, expressed as recombinant proteins, were active GTPases a...
Source: Molecular and Biochemical Parasitology - February 13, 2020 Category: Parasitology Source Type: research
The recognition of proteasomal receptors by Plasmodium falciparum DSK2
In this study, we have demonstrated how PfDsk2 and its recognition by proteasomal receptors differ from the mammalian system. Our crystal structure of unbound PfDsk2 UBL domain at 1.30 Å revealed an additional 310-helix compared to the human homolog, as well as a few significant differences in its putative binding interface with the proteasome receptors, PfRpn10 and PfRpn13. Moreover, the non-binding face of UBL showed a reversal of surface charge compared to HsDsk2 shuttle protein, instead resembling HOIL-like E3 ligase UBL domain. The affinity of the interaction with the proteasomal receptors remained similar to the h...
Source: Molecular and Biochemical Parasitology - February 13, 2020 Category: Parasitology Source Type: research
Molecular Basis for Hycanthone Drug Action in Schistosome Parasites
Publication date: Available online 3 February 2020Source: Molecular and Biochemical ParasitologyAuthor(s): Meghan Guzman, Anastasia Rugel, Reid S. Tarpley, Xiaohang Cao, Stanton F. McHardy, Philip T. LoVerde, Alexander B. TaylorAbstractHycanthone (HYC) is a retired drug formerly used to treat schistosomiasis caused by infection from Schistosoma mansoni and S. haematobium. Resistance to HYC was first observed in S. mansoni laboratory strains and in patients in the 1970s and the use of this drug was subsequently discontinued with the substitution of praziquantel (PZQ) as the single antischistosomal drug in the worldwide form...
Source: Molecular and Biochemical Parasitology - February 4, 2020 Category: Parasitology Source Type: research
Corrigendum to “Shotgun proteomics of Strongyloides venezuelensis infective third stage larvae: Insights into host-parasite interaction and novel targets for diagnostic” [Mol. Biochem. Parasitol. 235 (2020) 111249]
Publication date: Available online 3 February 2020Source: Molecular and Biochemical ParasitologyAuthor(s): Priscilla D.M. Fonseca, Marcelo A. Corral, Miguel Cosenza-Contreras, Dirce M.C.L. Meisel, Gessica B. Melo, Milena M.S. Antunes, Maria C.E. Santo, Ronaldo C.B. Gryschek, Julia M. Costa-Cruz, William Castro-Borges, Fabiana M. Paula (Source: Molecular and Biochemical Parasitology)
Source: Molecular and Biochemical Parasitology - February 4, 2020 Category: Parasitology Source Type: research
Proteomic analysis of two populations of Schistosoma mansoni-derived extracellular vesicles: 15k pellet and 120k pellet vesicles
Publication date: Available online 31 January 2020Source: Molecular and Biochemical ParasitologyAuthor(s): Desalegn Woldeyohannes Kifle, Mark S. Pearson, Luke Becker, Darren Pickering, Alex Loukas, Javier SotilloAbstractHelminth parasites secrete extracellular vesicles (EVs) into their environment that have potential roles in host-parasite communication, and thus represent potentially useful targets for novel control strategies. Here, we carried out a comprehensive proteomic analysis of two different populations of EVs – 15k pellet and 120k pellet EVs – from Schistosoma mansoni adult worms. We characterised the protein...
Source: Molecular and Biochemical Parasitology - February 1, 2020 Category: Parasitology Source Type: research
Observation of morphological changes of female osmiophilic bodies prior to Plasmodium gametocyte egress from erythrocytes
In this study, we investigate the dynamics of OBs in activated gametocytes of Plasmodium falciparum and Plasmodium yoelii using the female OB-specific marker protein, G377. After activation, female gametocyte OBs migrate to the parasite surface and fuse to form large vesicles beneath the parasite plasma membrane. At the marginal region of female gametocytes, fused vesicles secrete contents by exocytosis into the parasitophorous vacuole space, prior to parasite egress via the break-down of the erythrocyte membrane. This is the first detailed description of how proteins are transported through osmiophilic bodies. (Source: Mo...
Source: Molecular and Biochemical Parasitology - January 23, 2020 Category: Parasitology Source Type: research
Translation initiation factors GleIF4E2 and GleIF4A can interact directly with the components of the pre-initiation complex to facilitate translation initiation in Giardia lamblia
Publication date: Available online 20 January 2020Source: Molecular and Biochemical ParasitologyAuthor(s): Adebanjo Najeem Adedoja, Timothy McMahan, John Patrick Neal, Siddhartha Hamal Dhakal, Seetharama Jois, Daniel Romo, Ken Hull, Srinivas GarlapatiAbstractTranslation initiation factor eIF4F is essential for cap-dependent translation initiation in eukaryotes. eIF4F is a trimeric complex consisting of a scaffold protein eIF4G, cap-binding protein eIF4E and DEAD-box RNA helicase eIF4A. eIF4F binds to the 5’ cap structure of the mRNA through eIF4E and facilitates the binding of the preinitiation complex (PIC) via protein-...
Source: Molecular and Biochemical Parasitology - January 20, 2020 Category: Parasitology Source Type: research
Functional analysis of vasa/PL10-like genes in the ovary of Schistosoma mansoni
Publication date: Available online 17 January 2020Source: Molecular and Biochemical ParasitologyAuthor(s): Danielle E. Skinner, Anastas Popratiloff, Yousef N. Alrefaei, Victoria H. Mann, Gabriel Rinaldi, Paul J. BrindleyAbstractThe RNA helicase Vasa plays a pivotal role in the development of the germ line. To decipher the functional roles of vasa/PL10-like genes in the human blood fluke Schistosoma mansoni, we performed RNA interference followed by the analysis of the ovary in the adult female. Doubled stranded RNA targeting the schistosome vasa-like gene Smvlg1 reduced the volume of the ovary. Changes in morphology of the...
Source: Molecular and Biochemical Parasitology - January 17, 2020 Category: Parasitology Source Type: research
Molecular Detection of Trichostrongylus Species through PCR Followed by High Resolution Melt Analysis of ITS-2 rDNA Sequences
The objective of the present study was, for the first time, to develop a PCR-HRM assay for the detection of various animal Trichostrongylus spp. A pair of primers targeting the ITS-2 rDNA region of the Trichostrongylus spp. was designed for the development of the HRM assay. DNA samples were extracted from 30 adult worms of Trichostrongylus spp., the ITS-2-rDNA region was amplified using PCR, and the resultant products were sequenced and characterized. Afterwards, the PCR-HRM analysis was conducted to detect and discriminate Trichostrongylus spp. Molecular sequence analysis revealed that 24, 4, and 1 of the samples were T. ...
Source: Molecular and Biochemical Parasitology - January 17, 2020 Category: Parasitology Source Type: research
Editorial Board
Publication date: January 2020Source: Molecular and Biochemical Parasitology, Volume 235Author(s): (Source: Molecular and Biochemical Parasitology)
Source: Molecular and Biochemical Parasitology - January 10, 2020 Category: Parasitology Source Type: research
Shotgun proteomics of Strongyloides venezuelensis infective third stage larvae: Insights into host–parasite interaction and novel targets for diagnostics
This study presents an overview of the protein iL3 S. venezuelensis focusing on the diagnosis of strongyloidiasis. A total of 877 proteins were identified by shotgun proteomics. Many of these proteins are involved in different cellular processes, metabolic as well as structural maintenance. Our results point to a catalog of possible diagnostic targets for human strongyloidiasis and highlight the need for evaluation of uncharacterized proteins, especially the proteins within the CAP domain, transthyretin, and BTPI inhibitor domains, as a repertoire as yet unexplored in the context of strongyloidiasis diagnostic markers. We ...
Source: Molecular and Biochemical Parasitology - December 25, 2019 Category: Parasitology Source Type: research
Polarization of MTIP is a signature of gliding locomotion in Plasmodium ookinetes and sporozoites
Publication date: Available online 23 December 2019Source: Molecular and Biochemical ParasitologyAuthor(s): Inga Siden-Kiamos, Christian Goosmann, Carlos A. Buscaglia, Volker Brinkmann, Kai Matuschewski, Georgina N. MontagnaAbstractGliding motility and cell invasion are essential for the successful transmission of Plasmodium parasites. These processes rely on an acto-myosin motor located underneath the parasite plasma membrane. The Myosin A-tail interacting protein (MTIP) connects the class XIV myosin A (MyoA) to the gliding-associated proteins and is essential for assembly of the motor at the inner membrane complex. Here,...
Source: Molecular and Biochemical Parasitology - December 24, 2019 Category: Parasitology Source Type: research
Effect of cysteine peptidase inhibitor of Eudiplozoon nipponicum (Monogenea) on cytokine expression of macrophages in vitro
Publication date: Available online 23 December 2019Source: Molecular and Biochemical ParasitologyAuthor(s): Jana Ilgová, Lenka Kavanová, Katarína Matiašková, Jiří Salát, Martin KašnýAbstractThe gills of the common carp, whose mucosal surface belongs to the key defence mechanisms of piscine immunity, can be infested with both the larval and adult stage of Eudiplozoon nipponicum (Monogenea). Although on their own, monogeneans do not considerably compromise their hosts’ health status, fish with epithelial barriers damaged in parasite feeding and attachment sites are at an increased risk of bacterial challenge with...
Source: Molecular and Biochemical Parasitology - December 24, 2019 Category: Parasitology Source Type: research
