Selection and molecular characterization of cellulolytic–xylanolytic fungi from surface soil-biomass mixtures from Black Belt sites
Publication date: Available online 10 March 2015 Source:Microbiological Research Author(s): Benedict C. Okeke , Rosine W. Hall , Ananda Nanjundaswamy , M. Sue Thomson , Yasaman Deravi , Leah Sawyer , Andrew Prescott Plant biomass is an abundant renewable natural resource that can be transformed into chemical feedstocks. Enzymes used in saccharification of lignocellulosic biomass are a major part of biofuel production costs. A cocktail of cellulolytic and xylanolytic enzymes are required for optimal saccharification of biomass. Accordingly, thirty-two fungal pure cultures were obtained from surface soil-biomass mixtures c...
Source: Microbiological Research - April 24, 2015 Category: Infectious Diseases Source Type: research

Isolation and characterization of multifunctional Streptomyces species with antimicrobial, nematicidal and phytohormone activities from marine environments in Egypt
Publication date: Available online 10 March 2015 Source:Microbiological Research Author(s): Ferial M. Rashad , Hayam M. Fathy , Ayatollah S. El-Zayat , Ahlam M. Elghonaimy Different strategies have been employed for selective isolation of Streptomycetes from 20 marine samples varied in their biological nature. The recovery of Streptomycetes isolates (112) was influenced preferentially by different strategies; sediment samples were the best source of potential candidate Streptomycetes. All isolates exhibited antimicrobial activities with variable spectrum; the most promising isolates (31) were phenotypically characterized...
Source: Microbiological Research - April 24, 2015 Category: Infectious Diseases Source Type: research

Taxonomic and functional annotation of gut bacterial communities of Eisenia foetida and Perionyx excavatus
This study highlights the significance of differences in the inherent microbiome of these two earthworms in shaping the metagenome for effective degradation of different types of biomass under tropical conditions. (Source: Microbiological Research)
Source: Microbiological Research - April 24, 2015 Category: Infectious Diseases Source Type: research

The abundance of functional genes, cbbL, nifH, amoA and apsA, and bacterial community structure of intertidal soil from Arabian Sea
Publication date: Available online 17 March 2015 Source:Microbiological Research Author(s): Jitendra Keshri , Basit Yousuf , Avinash Mishra , Bhavanath Jha The Gulf of Cambay is a trumpet-shaped inlet of the Arabian Sea, located along the west coast of India and confronts a high tidal range with strong water currents. The region belongs to a semi-arid zone and saline alkaline intertidal soils are considered biologically extreme. The selected four soil types (S1–S4) were affected by salinity, alkalinity and sodicity. Soil salinity ranged from 20 to 126dS/m, soil pH 8.6–10.0 with high sodium adsorption ratio (S...
Source: Microbiological Research - April 24, 2015 Category: Infectious Diseases Source Type: research

Diversity and antifungal activity of the endophytic fungi associated with the native medicinal cactus Opuntia humifusa (Cactaceae) from the United States
Publication date: Available online 21 March 2015 Source:Microbiological Research Author(s): Alice F. Silva-Hughes , David E. Wedge , Charles L. Cantrell , Camila R. Carvalho , Zhiqiang Pan , Rita M. Moraes , Victor L. Madoxx , Luiz H. Rosa The endophytic fungal community associated with the native cactus Opuntia humifusa in the United States was investigated and its potential for providing antifungal compounds. A hundred-eight endophytic fungal isolates were obtained and identified by molecular methods into 17 different taxa of the genera Alternaria, Aureobasidium, Biscogniauxia, Cladosporium, Cryptococcus, Curvularia, D...
Source: Microbiological Research - April 24, 2015 Category: Infectious Diseases Source Type: research

Efficient production of safety-enhanced Escherichia coli ghosts by tandem expression of PhiX 174 mutant gene E and staphylococcal nuclease A gene
Publication date: Available online 21 March 2015 Source:Microbiological Research Author(s): Wenxing Zhu , Yuyu Zhang , Xinli Liu The application of bacterial ghosts as vaccines is limited because of their low lysis efficiency and production and the presence of pathogenic islands and/or antibiotic resistance genes within ghost preparations. To overcome these problems, a new lysis plasmid with fusion gene of mutant gene E and staphylococcal nuclease A gene (mE-L-SNA) were constructed and characterized. The new plasmid pBV-mELS could efficiently induce the genetic inactivation of Escherichia coli cultures, accompanied by th...
Source: Microbiological Research - April 24, 2015 Category: Infectious Diseases Source Type: research

Plant growth-promoting and antifungal activity of yeasts from dark chestnut soil
Publication date: Available online 21 March 2015 Source:Microbiological Research Author(s): Lyudmila V. Ignatova , Yelena V. Brazhnikova , Ramza Z. Berzhanova , Togzhan D. Mukasheva 538 yeast strains were isolated from dark chestnut soil collected from under the plants of the legume family (Fabaceae). The greatest number of microorganisms is found at soil depth 10–20cm. Among the 538 strains of yeast 77 (14.3%) strains demonstrated the ability to synthesize IAA. 15 strains were attributed to high IAA-producing yeasts (above 10μg/ml). The most active strains were YA05 with 51.7±2.1μg/ml of IAA and YR07 ...
Source: Microbiological Research - April 24, 2015 Category: Infectious Diseases Source Type: research

Evaluation of Zygosaccharomyces bailii BCV 08 as a co-starter in wine fermentation for the improvement of ethyl esters production
Publication date: April 2015 Source:Microbiological Research, Volume 173 Author(s): Juliano Garavaglia , Rosana de Cassia de Souza Schneider , Sandra Denise Camargo Mendes , Juliane Elisa Welke , Cláudia Alcaraz Zini , Elina Bastos Caramão , Patrícia Valente Zygosaccharomyces bailii BCV 08, a yeast isolated from red wine barrels in Brazil, was evaluated as co-starter in fermentations with Saccharomyces cerevisiae. Z. bailii BCV 08 was preliminarily shown to produce high levels of esters, and the production was optimized in bench and bioreactor scales using grape must. White wine vinifications were co...
Source: Microbiological Research - April 24, 2015 Category: Infectious Diseases Source Type: research

Characterization of cadmium-resistant endophytic fungi from Salix variegate Franch. in Three Gorges Reservoir Region, China
Publication date: Available online 2 April 2015 Source:Microbiological Research Author(s): Hongmei An , Yan Liu , Xinfei Zhao , Qian Huang , Shenhong Yuan , Xingyong Yang , Jinyan Dong The community and Cd-resistance of endophytic fungi from roots of Salix variegate Franch. collected from the water-level-fluctuation zone of Three Gorges Reservoir Region, China were investigated. A total of 53 strains were isolated and identified to 13 morphotaxa, in which Chromosporium, Fusarium and Gonatobotrys were dominant genera. Among them, 27 isolates were selected to measure their resistance to 0.02 mg·ml−1 Cd2+ and 1...
Source: Microbiological Research - April 24, 2015 Category: Infectious Diseases Source Type: research

Erratum to “Proteomic analysis of differential protein expression in Acidithiobacillus ferrooxidans cultivated in high potassium concentration” [Microbiol. Res. 168 (7) (2013) 455–460]
Publication date: Available online 4 April 2015 Source:Microbiological Research (Source: Microbiological Research)
Source: Microbiological Research - April 24, 2015 Category: Infectious Diseases Source Type: research

Geographical and longitudinal analysis of Listeria monocytogenes genetic diversity reveals its correlation with virulence and unique evolution
In this study, we performed multilocus sequence typing (MLST) and multi virulence locus sequence typing (MVLST) for 86 L. monocytogenes strains derived from 8 countries from 1926 to 2012 in order to better understand the molecular evolution and genetic characteristics of this pathogen. A total of 13 clonal complexes (CCs) were detected, of which CC1, CC2, CC3, CC7, CC9, CC4 are the most prevalent. Notably, polymorphism of housekeeping genes of isolates belong to CC1 (STs=47) increased more rapidly over the time. MLST-based phylogenetic analysis showed that serotype 1/2b and 4b strains had an “interval-type” evo...
Source: Microbiological Research - April 24, 2015 Category: Infectious Diseases Source Type: research

Saccharomyces cerevisiae: a novel and efficient biological control agent for Colletotrichum acutatum during pre-harvest
In this study, we evaluated the efficiency of six isolates of Saccharomyces cerevisiae in controlling Colletotrichum acutatum, the causal agent of postbloom fruit drop that occur in pre-harvest citrus. We analyzed the mechanisms of action involved in biological control such as: production of antifungal compounds, nutrient competition, detection of killer activity, and production of hydrolytic enzymes of the isolates of S. cerevisiae on C. acutatum and their efficiency in controlling postbloom fruit drop on detached citrus flowers. Our results showed that all six S. cerevisiae isolates produced antifungal compounds, compete...
Source: Microbiological Research - April 24, 2015 Category: Infectious Diseases Source Type: research

Structural and functional studies of BapC protein of Bordetella pertussis
In this study we have cloned newly identified member of autotransporter family BapC (B. pertussis autotransporter protein C); expressed it in Escherichia coli and characterized it for its different properties. We have also raised antisera to BapC protein; the antisera were used in immunofluorescence assay to determine the surface association of the protein. Results suggest that BapC in B. pertussis Taberman parent is surface exposed when compared with the respective BapC mutant. The neutralizing effect of anti-BapC serum was also evaluated in the presence of active complement proteins and results suggest that antiserum can...
Source: Microbiological Research - April 24, 2015 Category: Infectious Diseases Source Type: research

Isolation and identification of bacteria to improve the strength of concrete
The objective of this research work is to isolate and identify calcite precipitating bacteria and to check the suitability of these bacteria for use in concrete to improve its strength. Bacteria to be incorporated in concrete should be alkali resistant to endure the high pH of concrete and endospore forming to withstand the mechanical stresses induced in concrete during mixing. They must exhibit high urease activity to precipitate calcium carbonate in the form of calcite. Bacterial strains were isolated from alkaline soil samples of a cement factory and were tested for urease activity, potential to form endospores and prec...
Source: Microbiological Research - April 24, 2015 Category: Infectious Diseases Source Type: research

Genetic mutations potentially cause two novel NCF1 splice variants up-regulated in the mammary gland, blood and neutrophil of cows infected by Escherichia coli
In this study, we investigated the potential alternative splicing patterns, expression and splice-relevant single nucleotide polymorphisms (SNPs) of the bovine NCF1 gene to increase insights into its potential role against bovine mastitis caused by Escherichia coli infection. Using RT-PCR and clone sequencing methods, we found two novel splice variants designed as NCF1-TV1 (retained intron 6) and NCF1-TV2 (retained part of intron 8), respectively, encoding two putative truncated proteins (239AA and 283AA). Two splice variants were drastically up-regulated in the mastitis-infected cows’ mammary tissues, blood and neut...
Source: Microbiological Research - April 24, 2015 Category: Infectious Diseases Source Type: research

Stress response and expression of fluconazole resistance associated genes in the pathogenic yeast Candida glabrata deleted in the CgPDR16 gene
In this study, the entire chromosomal CgPDR16 ORF was replaced by the ScURA3 gene both in azole sensitive and azole resistant strains of Candida glabrata bearing a gain-of-function mutation in the CgPDR1 gene, and their responses to different stresses were assessed. The CgPDR16 deletion was found to sensitize the mutant strains to azole antifungals without changes in their osmo- and halotolerance. Fluconazole treated pdr16Δ mutant strains displayed a reduced expression of several genes involved in azole tolerance. The gain-of-function CgPDR1 allele as well as the cycloheximide and hydrogen peroxide treatments of cell...
Source: Microbiological Research - April 24, 2015 Category: Infectious Diseases Source Type: research

Genetic evidence for the involvement of glycosyltransferase PdmQ and PdmS in biosynthesis of pradimicin from Actinomadura hibisca
In this study, the exact role of each gene was evaluated by insertional inactivation and complementation studies. Analysis of the metabolite from both of the disruption mutants revealed abolishment of pradimicin A and complementation resulted in the recovery of production. After deletion of pdmQ, pradimicin B was found to accumulate, whereas deletion of pdmS resulted in the accumulation of aglycone of pradimicin. Together, these results suggest that pdmS is responsible for the attachment of thomosamine to form pradimicin B which in turn is glycosylated by pdmQ to form pradimicin A. These results allowed us to deduce the ex...
Source: Microbiological Research - April 24, 2015 Category: Infectious Diseases Source Type: research

Nocardiopsis species: Incidence, ecological roles and adaptations
Publication date: May 2015 Source:Microbiological Research, Volume 174 Author(s): Tahsin Bennur , Ameeta Ravi Kumar , Smita Zinjarde , Vaishali Javdekar Members of the genus Nocardiopsis are ecologically versatile and biotechnologically important. They produce a variety of bioactive compounds such as antimicrobial agents, anticancer substances, tumor inducers, toxins and immunomodulators. They also secrete novel extracellular enzymes such as amylases, chitinases, cellulases, β-glucanases, inulinases, xylanases and proteases. Nocardiopsis species are aerobic, Gram-positive, non-acid-fast, catalase-positive actinomyce...
Source: Microbiological Research - April 24, 2015 Category: Infectious Diseases Source Type: research

Distribution of the ompA-types among ruminant and swine pneumonic strains of Pasteurella multocida exhibiting various cap-locus and toxA patterns
Publication date: Available online 27 February 2015 Source:Microbiological Research Author(s): C. Vougidou , V. Sandalakis , A. Psaroulaki , V. Siarkou , E. Petridou , L. Ekateriniadou Pasteurella multocida is an important pathogen in food-producing animals and numerous virulence genes have been identified in an attempt to elucidate the pathogenesis of pasteurellosis. Currently, some of these genes including the capsule biosynthesis genes, the toxA and the OMPs-encoding genes have been suggested as epidemiological markers. However, the number of studies concerning ruminant isolates is limited, while, no attempt has ever ...
Source: Microbiological Research - February 27, 2015 Category: Infectious Diseases Source Type: research

Evaluation of Zygosaccharomyces bailii BVC 08 as a co-starter in wine fermentation for the improvement of ethyl esters production
Publication date: Available online 20 February 2015 Source:Microbiological Research Author(s): Juliano Garavaglia , Rosana de Cassia de Souza Schneider , Sandra Denise Camargo Mendes , Juliane Elisa Welke , Cláudia Alcaraz Zini , Elina Bastos Caramão , Patrícia Valente Zygosaccharomyces bailii BCV 08, a yeast isolated from red wine barrels in Brazil, was evaluated as co-starter in fermentations with Saccharomyces cerevisiae. Z. bailii BCV 08 was preliminarily shown to produce high levels of esters, and the production was optimized in bench and bioreactor scales using grape must. White wine vinificati...
Source: Microbiological Research - February 21, 2015 Category: Infectious Diseases Source Type: research

Genetic analysis of plant endophytic Pseudomonas putida BP25 and chemo-profiling of its antimicrobial volatile organic compounds
Publication date: Available online 17 February 2015 Source:Microbiological Research Author(s): Neelam Sheoran , Agisha Valiya Nadakkakath , Vibhuti Munjal , Aditi Kundu , Kesavan Subaharan , Vibina Venugopal , Suseelabhai Rajamma , Santhosh J. Eapen , Aundy Kumar Black pepper associated bacterium BP25 was isolated from root endosphere of apparently healthy cultivar Panniyur-5 that protected black pepper against Phytophthora capsici and Radopholus similis -the major production constraints. The bacterium was characterized and mechanisms of its antagonistic action against major pathogens elucidated. The polyphasic phenotypi...
Source: Microbiological Research - February 19, 2015 Category: Infectious Diseases Source Type: research

Isolation, characterization, and evaluation of multi-trait plant growth promoting rhizobacteria for their growth promoting and disease suppressing effects on ginger
In this study, 100 PGPR strains isolated from different varieties of ginger (Zingiber officinale Rosc.) were first characterized for their morphological, biochemical, and nutrient mobilization traits in vitro. The PGPR were also screened in vitro for inhibition of Pythium myriotylum causing soft rot in ginger. Results revealed that only five PGPR showed >70% suppression of P. myriotylum. These 5 PGPR viz., GRB (Ginger rhizobacteria) 25- Burkholderia cepacia, GRB35- Bacillus amyloliquefaciens; GRB58- Serratia marcescens; GRB68–Serratia marcescens; GRB91-Pseudomonas aeruginosa were used for further growth promot...
Source: Microbiological Research - February 16, 2015 Category: Infectious Diseases Source Type: research

Melting curve analysis of a groEL PCR fragment for the rapid genotyping of strains belonging to the Lactobacillus casei group of species
In conclusion, the melting curve analysis developed in this study is demonstrably a simple, labor-saving, and rapid strategy obtain the genotyping of a bacterial isolate and simultaneously potentially confirm its affiliation to the L. casei group of species. The application of this method to a larger collection of strains may validate the possibility to use the proposed HRMa protocol for the taxonomic discrimination of L. casei group of species. In general, this study suggests that HRMa can be a suitable technique for the genetic typization of Lactobacillus strains. (Source: Microbiological Research)
Source: Microbiological Research - February 16, 2015 Category: Infectious Diseases Source Type: research

Generation of the natamycin analogs by gene engineering of natamycin biosynthetic genes in Streptomyces chattanoogensis L10
Publication date: Available online 7 February 2015 Source:Microbiological Research Author(s): Shui-Ping Liu , Peng-Hui Yuan , Yue-Yue Wang , Xiao-Fang Liu , Zhen-Xing Zhou , Qing-ting Bu , Pin Yu , Hui Jiang , Yong-Quan Li The polyene antibiotic natamycin is widely used as an antifungal agent in both human therapy and the food industry. Here we obtained four natamycin analogs with high titers, including two new compounds, by engineering of six post-polyketide synthase (PKS) tailoring enzyme encoding genes in a natamycin industrial producing strain, Streptomyces chattanoogensis L10. Precise analysis of S. chattanoogensis ...
Source: Microbiological Research - February 9, 2015 Category: Infectious Diseases Source Type: research

Bacterial communities in thermophilic H2-producing reactors investigated using 16S rRNA 454 pyrosequencing
In this study, the composition and diversity of the bacterial community in thermophilic H2-producing reactors fed with glucose were investigated using pyrosequencing. The H2-producing experiments in batch were conducted using 0.5 and 2.0g l−1 glucose at 55o C. Under the two conditions, the H2 production and yield were 1.3 and 1.6mol H2 mol glucose−1, respectively. Acetic, butyric, iso-butyric, lactic and propionic acids were detected in the two reactors. The increase in substrate concentration favored a high H2 yield. In this reactor, a predominance of acetic and iso-butyric acids, 27.7% and 40%, were measured,...
Source: Microbiological Research - February 9, 2015 Category: Infectious Diseases Source Type: research

Identification of BagI as a positive transcriptional regulator of bagremycin biosynthesis in engineered Streptomyces sp. Tü 4128
In this study, we obtained a bagI deletion mutant by in-frame deletion. The assays of bagI-deletion and complementation strains revealed that it was essential to bagremycin biosynthesis. Secondly, the impact of bagI mutants on mycelial growth, sporulation and pigment yields were explored throughout secondary metabolism. SEM images displayed that bagI mutation delayed the mycelium growth and sporulation and reduced pigment yields. Moreover, the yields of bagremycin A and B increased 2.5-fold and 2.6-fold in bagI-overexpressed strain compared to WT. Thirdly, we investigated genes associated with bagremycin biosynthesis by re...
Source: Microbiological Research - February 9, 2015 Category: Infectious Diseases Source Type: research

The rad2 Gene of Haloarchaeum Halobacterium salinarum is Functional in the Repair of Ultraviolet Light Induced DNA Photoproducts
In this study, for the first time antisense RNA is used for functional analysis of a gene in H. salinarum and it is shown that antisense RNA could be used as a reliable genetic tool for understanding of the archaeal genetics. (Source: Microbiological Research)
Source: Microbiological Research - February 9, 2015 Category: Infectious Diseases Source Type: research

Phylogenetic diversity and biological activity of culturable Actinobacteria isolated from freshwater fish gut microbiota
This study demonstrates the significant diversity of Actinobacteria in the fish gut microbiota and it's potential to produce biologically active compounds. (Source: Microbiological Research)
Source: Microbiological Research - January 24, 2015 Category: Infectious Diseases Source Type: research

Identification of the microbial community composition and structure of coal-mine wastewater treatment plants
Publication date: Available online 23 January 2015 Source:Microbiological Research Author(s): Qiao Ma , Yuan-Yuan Qu , Xu-Wang Zhang , Wen-Li Shen , Zi-Yan Liu , Jing-Wei Wang , Zhao-Jing Zhang , Ji-Ti Zhou The wastewater from coal-mine industry varies greatly and is resistant to biodegradation for containing large quantities of inorganic and organic pollutants. Microorganisms in activated sludge are responsible for the pollutants removal, whereas the microbial community composition and structure are far from understood. In the present study, the sludges from five coal-mine wastewater treatment plants were collected and ...
Source: Microbiological Research - January 24, 2015 Category: Infectious Diseases Source Type: research

Bacterial growth rates are influenced by cellular characteristics of individual species when immersed in electromagnetic fields
Publication date: Available online 19 January 2015 Source:Microbiological Research Author(s): Lucas W.E. Tessaro , Nirosha J. Murugan , Michael A. Persinger Previous studies have shown that exposure to extremely low-frequency electromagnetic fields (ELF-EMFs) have negative effects on the rate of growth of bacteria. In the present study, two Gram-positive and two Gram-negative species were exposed to six magnetic field conditions in broth cultures. Three variations of the ‘Thomas’ pulsed frequency-modulated pattern; a strong-static “puck” magnet upwards of 5000 G in intensity; a pair of these magne...
Source: Microbiological Research - January 20, 2015 Category: Infectious Diseases Source Type: research

Plasmid-borne cadmium resistant determinants are associated with the susceptibility of Listeria monocytogenes to bacteriophage
In this study, we determined the prevalence and molecular profile of plasmids in food isolates of L. monocytogenes and examined the contribution of four plasmid-borne cadmium-resistant genes to the susceptibility of L. monocytogenes to bacteriophage infection. The results showed that plasmids were isolated from 55% (11/20) of the isolates and the plasmids exhibited 10 molecular types as determined by restriction enzyme digestion. Furthermore, 65% and 15% of the isolates were tolerant to cadmium and benzalkonium chloride (BC), respectively. All the BC-resistant isolates were resistant to cadmium. The prevalence of predicted...
Source: Microbiological Research - January 20, 2015 Category: Infectious Diseases Source Type: research

Paenibacillus yonginensis DCY84T induces changes in Arabidopsis thaliana gene expression against aluminum, drought, and salt stress
Publication date: Available online 19 January 2015 Source:Microbiological Research Author(s): Johan Sukweenadhi , Yeon-Ju Kim , Eul-Su Choi , Sung-Cheol Koh , Sang-Won Lee , Yu-Jin Kim , Deok Chun Yang Current agricultural production methods, for example the improper use of chemical fertilizers and pesticides, create many health and environmental problems. Use of plant growth-promoting bacteria (PGPB) for agricultural benefits is increasing worldwide and also appears to be a trend for the future. There is possibility to develop microbial inoculants for use in agricultural biotechnology, based on these beneficial plant-mi...
Source: Microbiological Research - January 20, 2015 Category: Infectious Diseases Source Type: research

Silver nanoparticles mediated altered gene expression of melanin biosynthesis genes in Bipolaris sorokiniana
Publication date: Available online 17 January 2015 Source:Microbiological Research Author(s): Sandhya Mishra , H.B. Singh Melanin production in many fungal phytopathogens has been investigated to play direct or indirect role in pathogenesis. However, in Bipolaris sorokiniana, the spot blotch pathogen of wheat, much less is known about the role melanin play in pathogenesis. As an extension of our previous report, the present study aims to investigate the plausible association between melanin production and virulence factor in B. sorokiniana. In the previous study, we carried out analysis on the antifungal efficacy of bios...
Source: Microbiological Research - January 18, 2015 Category: Infectious Diseases Source Type: research

Mannose-Binding Lectin Impairs Leptospira Activity through the Inhibitory Effect on the Motility of Cell
In this study, the effect of mannose-binding lectin to Leptospira was quantitatively investigated by measuring some kinematic parameters, to discover the mechanism behind MBL-mediated immune responses during leptospiral infection. The results showed that mannose-binding lectin is capable of inhibiting the motility of Leptospira by transforming free swimming cells to tumbled rotating cells, resulted in the increase number of rotating cells. Otherwise, decrease in rotation rate of rotating cell has been observed. However, the swimming speed of swimming Leptospira cells showed no observable change under the effect of MBL. The...
Source: Microbiological Research - January 16, 2015 Category: Infectious Diseases Source Type: research

Influence of cyanobacterial inoculation on the culturable microbiome and growth of rice
Publication date: Available online 3 January 2015 Source:Microbiological Research Author(s): Himani Priya , Radha Prasanna , Balasubramanian Ramakrishnan , Ngangom Bidyarani , Santosh Babu , Shobit Thapa , Nirmal Renuka Rice plants are selective with their associations with bacteria that are beneficial for growth, nutrient uptake, exhibit induced resistance or antagonism towards pathogens. Cyanobacteria as bioinoculants are known to promote the growth and health of rice plants. The present investigation was aimed at understanding whether and how cyanobacterial (Calothrix elenkinii) inoculation influenced the rice plant g...
Source: Microbiological Research - January 16, 2015 Category: Infectious Diseases Source Type: research

The antimicrobial peptide cathelicidin-BF could be a potential therapeutic for Salmonella Typhimurium infection
Publication date: Available online 3 January 2015 Source:Microbiological Research Author(s): Xi Xia , Lin Zhang , Yizhen Wang Resistance is increasing to several critical antimicrobials used to treat Salmonella Typhimurium infection, urging people to search for new antimicrobial agents. In this work, we reported the possibility of a potent antimicrobial peptide cathelicidin-BF found in the venom of the snake Bungarus fasciatus in treating Salmonella Typhimurium infection. We tested its activity in biological fluids and in vivo using a mouse model of Salmonella Typhimurium infection, and examined the effect of cathelicidi...
Source: Microbiological Research - January 16, 2015 Category: Infectious Diseases Source Type: research

Arachis hypogaea PGPR isolated from Argentine soil modifies its lipids components in response to temperature and salinity
Publication date: Available online 3 January 2015 Source:Microbiological Research Author(s): Natalia S. Paulucci , Lucas A. Gallarato , Yanina B. Reguera , Julio C. Vicario , Adriana B. Cesari , Mirta B. García de Lema , Marta S. Dardanelli The aim of this work was to clarify the mechanism related to plant growth promoting of a bacterial strain (L115) isolated from Arachis hypogaea rhizospheres and the effects of high growth temperature and salinity on phospholipids and fatty acids composition. L115 was isolated from peanut rhizospheres and identified according to the sequence analysis of the 16S rRNA gene. Phenot...
Source: Microbiological Research - January 16, 2015 Category: Infectious Diseases Source Type: research

Glycine metabolism and anti-oxidative defence mechanisms in Pseudomonas fluorescens
Publication date: Available online 5 January 2015 Source:Microbiological Research Author(s): Azhar Alhasawi , Zachary Castonguay , Nishma D. Appanna , Christopher Auger , Vasu D. Appanna The role of metabolism in anti-oxidative defence is only now beginning to emerge. Here, we show that the nutritionally-versatile microbe, Pseudomonas fluorescens, reconfigures its metabolism in an effort to generate NADPH, ATP and glyoxylate in order to fend off oxidative stress. Glyoxylate was produced predominantly via the enhanced activities of glycine dehydrogenase-NADP+ (GDH), glycine transaminase (GTA) and isocitrate lyase (ICL) in...
Source: Microbiological Research - January 16, 2015 Category: Infectious Diseases Source Type: research

Functional analysis of the uropathogenic Escherichia coli R049 gene
In conclusion, R049 expression in UPEC132 is related to the early innate immune and inflammatory responses in UPEC-infected hosts. This work lays the foundation for further research on anti-infective immunity against UPEC. (Source: Microbiological Research)
Source: Microbiological Research - January 16, 2015 Category: Infectious Diseases Source Type: research

Multi -omics and metabolic modelling pipelines: challenges and tools for systems microbiology
Publication date: Available online 7 January 2015 Source:Microbiological Research Author(s): Marco Fondi , Pietro Liò Integrated -omics approaches are quickly spreading across microbiology research labs, leading to i) the possibility of detecting previously hidden features of microbial cells like multi-scale spatial organisation and ii) tracing molecular components across multiple cellular functional states. This promises to reduce the knowledge gap between genotype and phenotype and poses new challenges for computational microbiologists. We underline how the capability to unravel the complexity of microbial life ...
Source: Microbiological Research - January 16, 2015 Category: Infectious Diseases Source Type: research

Composition, function, and regulation of T6SS in Pseudomonas aeruginosa
This article reviews the latest progress in the structure, effector proteins, biological function, and regulation mechanisms of P. aeruginosa T6SS. (Source: Microbiological Research)
Source: Microbiological Research - January 16, 2015 Category: Infectious Diseases Source Type: research

Mitochondria Thioredoxin's Backup Role in Oxidative Stress Resistance in Trichoderma reesei
In this study, we identified a Trx-encoding gene, Trtrx1. The protein expressed located specifically in the mitochondria as verified by the fluorescence signals of TrTRX1-EGFP. TrTRX1 can catalyze the reduction of insulin disulfides by dithiothreitol (DTT). Loss of Trtrx1 however, did not lead to either significant morphology abnormality under normal and oxidative stress condition, or detectable difference in reactive oxygen species (ROS) resistance. The unchanged GSH amount in Trtrx1 deletion strain under normal condition and slight increase under oxidative stress condition, as well as the interplay between Trx and GSH sy...
Source: Microbiological Research - January 16, 2015 Category: Infectious Diseases Source Type: research

1,3-Propanediol production by new recombinant Escherichia coli containing genes from pathogenic bacteria
Publication date: February 2015 Source:Microbiological Research, Volume 171 Author(s): Hanna Przystałowska , Joanna Zeyland , Daria Szymanowska-Powałowska , Marlena Szalata , Ryszard Słomski , Daniel Lipiński 1,3-Propanediol (1,3-PDO) is an organic compound, which is a valuable intermediate product, widely used as a monomer for synthesizing biodegradable polymers, increasing their strength; as well as an ingredient of textile, cosmetic and medical products. 1,3-PDO is mostly synthesized chemically. Global companies have developed technologies for 1,3-PDO synthesis from petroleum products such as acrolein and ethylene...
Source: Microbiological Research - January 16, 2015 Category: Infectious Diseases Source Type: research

Mutational analysis of GlnB residues critical for NifA activation in Azospirillum brasilense
Publication date: Available online 31 December 2014 Source:Microbiological Research Author(s): Juliana Inaba , Jeremy Thornton , Luciano Fernandes Huergo , Rose Adele Monteiro , Giseli Klassen , Fábio de Oliveira Pedrosa , Mike Merrick , Emanuel Maltempi de Souza PII proteins are signal transduction that sense cellular nitrogen status and relay this signals to other targets. Azospirillum brasilense is a nitrogen fixing bacterium, which associates with grasses and cereals promoting beneficial effects on plant growth and crop yields. A. brasilense contains two PII encoding genes, named glnB and glnZ. In this paper, ...
Source: Microbiological Research - January 1, 2015 Category: Infectious Diseases Source Type: research

Improvement of exopolysaccharide production in Lactobacillus casei LC2W by overexpression of NADH oxidase gene
Publication date: Available online 22 December 2014 Source:Microbiological Research Author(s): Nan Li , Yuanlong Wang , Ping Zhu , Zhenmin Liu , Benheng Guo , Jing Ren Lactobacillus casei LC2W is an exopolysaccharide (EPS)-producing strain with probiotic effects. To investigate the regulation mechanism of EPS biosynthesis and to improve EPS production through cofactor engineering, a H2O-forming NADH oxidase gene was cloned from Streptococcus mutans and overexpressed in L. casei LC2W under the control of constitutive promoter P23. The recombinant strain LC-nox exhibited 0.854 U/mL of NADH oxidase activity, which was eleva...
Source: Microbiological Research - December 23, 2014 Category: Infectious Diseases Source Type: research

Functional analysis of the class II hydrophobin gene HFB2-6 from the biocontrol agent Trichoderma asperellum ACCC30536
Publication date: Available online 22 December 2014 Source:Microbiological Research Author(s): Ying Huang , Gulijimila Mijiti , Zhiying Wang , Wenjing Yu , Haijuan Fan , Rongshu Zhang , Zhihua Liu A class II hydrophobin gene, HFB2-6, was cloned from Trichoderma asperellum ACCC30536 and its biocontrol function was studied. According to our previous transcriptome data, six of the eight class II hydrophobin genes were obviously differential expression in four inducing conditions, especially the gene HFB2-6. Moreover, HFB2-6 proven to be differentially transcribed under eight different treatments. HFB2-6 transcripts were up-...
Source: Microbiological Research - December 23, 2014 Category: Infectious Diseases Source Type: research

1,3-propanediol production by new recombinant e. coli containing genes from pathogenic bacteria
Publication date: Available online 23 December 2014 Source:Microbiological Research Author(s): Hanna Przystałowska , Joanna Zeyland , Daria Szymanowska-Powałowska , Marlena Szalata , Ryszard Słomski , Daniel Lipiński 1,3-Propanediol (1,3-PDO) is an organic compound, which is a valuable intermediate product, widely used as a monomer for synthesizing biodegradable polymers, increasing their strength; as well as an ingredient of textile, cosmetic and medical products. 1,3-PDO is mostly synthesized chemically. Global companies have developed technologies for 1,3-PDO synthesis from petroleum products such as acrolein and ...
Source: Microbiological Research - December 23, 2014 Category: Infectious Diseases Source Type: research

Detection of major diarrheagenic bacterial pathogens by multiplex PCR panels
Publication date: Available online 12 December 2014 Source:Microbiological Research Author(s): Åsa Sjöling , Leila Sadeghipoorjahromi , Daniel Novak , Joshua Tobias Diarrheal diseases remain a major threat to the youngest population in low- and middle-income countries. The main bacterial pathogens causing diarrhea are diarrheagenic Escherichia coli (DEC) that consists of enteroaggregative (EAEC), enteropathogenic (EPEC), enterotoxigenic (ETEC), enterohemorrhagic EHEC and enteroinvasive E. coli (EIEC), Salmonella, Shigella spp. (S. dysenteria, S. sonnei, S. flexneri) Campylobacter (C. coli, C. jejuni), Vibrio (...
Source: Microbiological Research - December 20, 2014 Category: Infectious Diseases Source Type: research

Polyphosphate kinase of Lysinibacillus sphaericus and its effects on accumulation of polyphosphate and bacterial growth
Publication date: Available online 12 December 2014 Source:Microbiological Research Author(s): Tingyu Shi , Yong Ge , Ni Zhao , Xiaomin Hu , Zhiming Yuan Lysinibacillus sphaericus produces parasporal crystal proteins (Bin toxins) that are specifically toxic to Culex and Anopheles mosquito larvae. It has long persistence in mosquito breeding sites due to the recycle of spores in mosquito cadaver and certain environments. This bacterium is unable to metabolize sugar (except N-acetylglucosamine) but can use proteinaceous materials. Previous studies have shown that inorganic polyphosphate (polyP) is essential for cell growth...
Source: Microbiological Research - December 20, 2014 Category: Infectious Diseases Source Type: research

The Adh1 gene of the fungus Metarhizium anisopliae is expressed during insect colonization and required for full virulence
Publication date: Available online 5 December 2014 Source:Microbiological Research Author(s): Olga Alicia Callejas-Negrete , Juan Carlos Torres-Guzmán , Israel Enrique Padilla Guerrero , Ulises Esquivel-Naranjo , Maria Fernanda Padilla Ballesteros , Adriana García Tapia , Augusto Schrank , Eduardo Salazar-Solís , Félix Gutiérrez-Corona , Gloria Angélica González-Hernández Zymography of alcohol dehydrogenase (ADH) activity in the entomopathogenic fungus Metarhizium anisopliae grown under various conditions revealed that micro-aerobic growth was associated with increa...
Source: Microbiological Research - December 6, 2014 Category: Infectious Diseases Source Type: research