Cellulolytic potential of probiotic Bacillus Subtilis AMS6 isolated from traditional fermented soybean (Churpi): An in-vitro study with regards to application as an animal feed additive
Publication date: Available online 23 March 2016 Source:Microbiological Research Author(s): Ajay K. Manhar, Yasir Bashir, Devabrata Saikia, Dhrubajyoti Nath, Kuldeep Gupta, Bolin K. Konwar, Rahul Kumar, Nima D. Namsa, Manabendra Mandal The aim of the present study is to evaluate the probiotic attributes of Bacillus subtilis AMS6 isolated from fermented soybean (Churpi). This isolate exhibited tolerance to low pH (pH 2.0) and bile salt (0.3%), capability to autoaggregate and coaggregate. AMS6 also showed highest antibacterial activity against the pathogenic indicator strain Salmonella enterica typhimurium (MTCC 1...
Source: Microbiological Research - March 23, 2016 Category: Infectious Diseases Source Type: research

Wheat seeds harbour bacterial endophytes with potential as plant growth promoters and biocontrol agents of Fusarium graminearum
Publication date: Available online 9 March 2016 Source:Microbiological Research Author(s): Silvana Díaz Herrera, Cecilia Grossi, Myriam Zawoznik, María Daniela Groppa The role of endophytic communities of seeds is still poorly characterised. The purpose of this work was to survey the presence of bacterial endophytes in the seeds of a commercial wheat cultivar widely sown in Argentina and to look for plant growth promotion features and biocontrol abilities against F. graminearum among them. Six isolates were obtained from wheat seeds following a culture-dependent protocol. Four isolates were assignated t...
Source: Microbiological Research - March 10, 2016 Category: Infectious Diseases Source Type: research

The effect of berberine hydrochloride on Enterococcus faecalis biofilm formation and dispersion in vitro
In this study, 99 clinical isolates from the urine samples of patients with urinary tract infections (UTIs) were collected and identified. Ten strains of E. faecalis with biofilm formation ability were studied. BBH inhibited E. faecalis biofilm formation and promoted the biofilm dispersion of E. faecalis. In addition, sortase A and esp expression levels were elevated during early E. faecalis biofilm development, whereas BBH significantly reduced their expression levels. The results of this study indicated that BBH effectively prevents biofilm formation and promotes biofilm dispersion in E. faecalis, most likely by inhibiti...
Source: Microbiological Research - March 10, 2016 Category: Infectious Diseases Source Type: research

Large-scale gene expression profiling reveals physiological response to deletion of chaperone dnaKJ in Escherichia coli
This study is the first to systematically analyze the alterations at the transcriptional level in vivo in deletion of dnaKJ. Fatty acid methyl esters analysis indicated that the amount of unsaturated fatty acid sharply increased and subcellular location prediction analysis showed a marked decrease in transcription of inner-membrane protein genes, which might have triggered the development of aberrant cell shape and susceptibility for some antibiotics in the △dnaKJ strain. (Source: Microbiological Research)
Source: Microbiological Research - March 6, 2016 Category: Infectious Diseases Source Type: research

GH10 XynA is the main xylanase identified in the crude enzymatic extract of Paenibacillus sp. A59 when grown on xylan or lignocellulosic biomass
Publication date: Available online 3 March 2016 Source:Microbiological Research Author(s): Silvina Ghio, Ester M. Insani, Florencia E. Piccinni, Paola M. Talia, Daniel H. Grasso, Eleonora Campos A novel bacterial isolate with polysaccharides degrading activity was identified as Paenibacillus sp., and named Paenibacillus sp. A59. Even though it is a strict mesophile, optimal xylanase activity of the crude enzymatic extract was achieved between 50°C and 70°C and more than 60% of the activity was retained after incubation for 48h at 50°C, indicating thermotolerance of the enzymes involved. The extract was ...
Source: Microbiological Research - March 4, 2016 Category: Infectious Diseases Source Type: research

Isolation and Characterization of a new Bacillus thuringiensis strain with a promising toxicity against Lepidopteran pests
Publication date: Available online 22 February 2016 Source:Microbiological Research Author(s): Hanen Boukedi, Sameh Sellami, Sonia Ktari, Najeh Belguith-Ben Hassan, Tahya Sellami-Boudawara, Slim Tounsi, Lobna Abdelkefi-Mesrati Insecticides derived from Bacillus thuringiensis are gaining worldwide importance as environmentally desirable alternatives to chemicals for the control of pests in public health and agriculture. Isolation and characterization of new strains with higher and broader spectrum of activity is an ever growing field. In the present work, a novel Tunisian B. thuringiensis isolate named BLB459 was c...
Source: Microbiological Research - February 22, 2016 Category: Infectious Diseases Source Type: research

Inactivation of Glutamate Racemase (MurI) Eliminates Virulence in Streptococcus mutans
Publication date: Available online 11 February 2016 Source:Microbiological Research Author(s): Jianying Zhang, Jia Liu, Junqi Ling, Zhongchun Tong, Yun Fu, Min Liang Inhibition of enzymes required for bacterial cell wall synthesis is often lethal or leads to virulence defects. Glutamate racemase (MurI), an essential enzyme in peptidoglycan biosynthesis, has been an attractive target for therapeutic interventions. Streptococcus mutans, one of the many etiological factors of dental caries, possesses a series of virulence factors associated with cariogenicity. However, little is known regarding the mechanism by which ...
Source: Microbiological Research - February 12, 2016 Category: Infectious Diseases Source Type: research

Effect of the glycosyltransferases on the capsular polysaccharide synthesis of Streptococcus suis serotype 2
In this study, four genes (cps2E, cps2G, cps2J and cps2L) encoding different glycosyltransferases involved in CPS synthesis were researched in S. suis 2. Four deletion mutants (Δcps2E, Δcps2G, Δcps2J and Δcps2L) with their CPS incomplete and their sialic acid content significantly decreased were constructed in S. suis 2 SC19. All these four mutant strains showed enhanced adhesion to Hep-2 cells and increased sensitivity to phagocytosis. Flow cytometric analysis also revealed that these four mutants were more susceptible to the attack by the complement system. In a mouse model of infection, the mutan...
Source: Microbiological Research - February 6, 2016 Category: Infectious Diseases Source Type: research

Biosynthesis of 3″-demethyl-gentamicin C components by genN disruption strain of Micromonospora echinospora and test their antimicrobial activities in vitro
In this study, 3″-N-demethyl gentamicin C1a was purified from the broth of disruption strain, and its structure was elucidated using MS and NMR. Besides 3″-N-demethyl products corresponding to gentamicin C1a, C2, and C2a, two 3″-N-demethyl products corresponding to gentamicin C1 were detected, which were concluded as C-6′ epimers originating from decreased substrate specificity of 6'-N-methyltransferase. To explore the effects of 3″-N-methyl on gentamicin antimicrobial activity, antimicrobial activity of these demethyl gentamicin analogues were tested in vitro. 3″-N-demethyl gentamicin c...
Source: Microbiological Research - January 28, 2016 Category: Infectious Diseases Source Type: research

Rare actinomycetes Nocardia caishijiensis and Pseudonocardia carboxydivorans as endophytes, their bioactivity and metabolites evaluation
This study reports the rare actinomycetes N. caishijiensis and P. carboxydivorans as endophytes and evaluates their bioactive metabolites. (Source: Microbiological Research)
Source: Microbiological Research - January 25, 2016 Category: Infectious Diseases Source Type: research

Biocontrol activity of Paenibacillus polymyxa AC-1 against Pseudomonas syringae and its interaction with Arabidopsis thaliana
This study shows that AC-1 is an effective biocontrol agent to suppress Pseudomonas syringae growth, possibly owing to its colonization patterns as a leaf-inhabiting endophyte. The results showed in this work will help to expand our understanding of the mode of action of AC-1 as a biological control agent and consequently, its application in agriculture. (Source: Microbiological Research)
Source: Microbiological Research - January 23, 2016 Category: Infectious Diseases Source Type: research

High-throughput transcriptome sequencing analysis provides preliminary insights into the biotransformation mechanism of Rhodopseudomonas palustris treated with alpha-rhamnetin-3-rhamnoside
Conclusion We present the first comparative transcriptome profiles of RPB and RPS samples to facilitate elucidation of the molecular mechanism of biotransformation in R. palustris. Furthermore, we propose two putative ARR biotransformation mechanisms in R. palustris. These analytical results represent a useful genomic resource for in-depth research into the molecular basis of biotransformation and genetic modification in R. palustris. (Source: Microbiological Research)
Source: Microbiological Research - January 23, 2016 Category: Infectious Diseases Source Type: research

The degradation of RcsA by ClpYQ(HslUV) protease in Escherichia coli
Publication date: Available online 16 January 2016 Source:Microbiological Research Author(s): Chun-Yang Chang, Hui-Ting Hu, Chih-Hsuan Tsai, Whei-Fen Wu In Escherichia coli, RcsA, a positive activator for transcription of cps (capsular polysaccharide synthesis) genes, is degraded by the Lon protease. In lon mutant, the accumulation of RcsA leads to overexpression of capsular polysaccharide. In a previous study, overproduction of ClpYQ(HslUV) protease represses the expression of cpsB:lacZ, but there has been no direct observation demonstrating that ClpYQ degrades RcsA. By means of a MBP-RcsA fusion protein, we showed ...
Source: Microbiological Research - January 18, 2016 Category: Infectious Diseases Source Type: research

The New Flagella-associated Collagen-like Proteins ClpB and ClpC of Bacillus amyloliquefaciens FZB42 are Involved in Bacterial Motility
Publication date: Available online 23 December 2015 Source:Microbiological Research Author(s): Xia Zhao, Ruoyu Wang, Qianhan Shang, Haiting Hao, Yuyao Li, Yubao Zhang, Zhihong Guo, Yun Wang, Zhongkui Xie Collagen-like proteins (CLPs) share the distinctive Gly-X-Thr repeating amino acid sequence of animal collagens, and contain N- and C-terminal domain making a collagen-like structure in Bacillus amyloliquefaciens FZB42, a plant growth-promoting rhizobacterium. Our previous study demonstrated that CLPs play important roles in biofilm construction and adherence to the surfaces on plant roots. However, bacterial lo...
Source: Microbiological Research - December 25, 2015 Category: Infectious Diseases Source Type: research

Efficacies of quorum sensing inhibitors, piericidin A and glucopiericidin A, produced by Streptomyces xanthocidicus KPP01532 for the control of potato soft rot caused by Erwinia carotovora subsp. atroseptica
Publication date: Available online 21 December 2015 Source:Microbiological Research Author(s): Ji Eun Kang, Jae Woo Han, Byeong Jun Jeon, Beom Seok Kim To discover potential inhibitors of the quorum sensing (QS) system, a library of microbial culture extracts was screened with Chromobacterium violaceum CV026 strain. The culture extract of Streptomyces xanthocidicus KPP01532 contained quorum-sensing inhibitors (QSIs) of the CV026 strain. The active constituents of the culture extract of strain KPP01532 were purified using a series of chromatographic procedures, and based on data from NMR and mass spectroscopy, pierici...
Source: Microbiological Research - December 22, 2015 Category: Infectious Diseases Source Type: research

Enhancement of drought stress tolerance in crops by plant growth promoting rhizobacteria
Publication date: Available online 17 December 2015 Source:Microbiological Research Author(s): Sai Shiva Krishna Prasad Vurukonda, Sandhya Vardharajula, Manjari Shrivastava, Ali SkZ Drought is one of the major constraints on agricultural productivity worldwide and is likely to further increase. Several adaptations and mitigation strategies are required to cope with drought stress. Plant growth promoting rhizobacteria (PGPR) could play a significant role in alleviation of drought stress in plants. These beneficial microorganisms colonize the rhizosphere/endo-rhizosphere of plants and impart drought tolerance by produc...
Source: Microbiological Research - December 17, 2015 Category: Infectious Diseases Source Type: research

Identification of genes differentially expressed during early interactions between the stem rot fungus (Sclerotium rolfsii) and peanut (Arachis hypogaea) cultivars with increasing disease resistance levels
This study aimed to identify differentially expressed genes associated with peanut resistance and fungal virulence. Four peanut cultivars (A100-32, Georgia Green, GA-07W and York) with increasing resistance levels were inoculated with a virulent S. rolfsii strain to study the early plant-pathogen interaction. 454 sequencing was performed on RNAs from infected tissue collected at 4 days post inoculation, generating 225,793 high-quality reads. Normalized read counts and fold changes were calculated and statistical analysis used to identify differentially expressed genes. Several genes identified as differential in the RNA-se...
Source: Microbiological Research - December 17, 2015 Category: Infectious Diseases Source Type: research

Antagonistic activity of fungi of Olea europaea L. against Colletotrichum acutatum
Publication date: Available online 12 December 2015 Source:Microbiological Research Author(s): Miguel C. Landum, Maria do Rosário Félix, Joana Alho, Raquel Garcia, Maria João Cabrita, Fernando Rei, Carla M.R. Varanda Fungi naturally present in olive trees were identified and tested for their antagonistic potential against C. acutatum. A total of 14 isolates were identified, 12 belonged to genera Alternaria, Epicoccum, Fusarium, Aspergillus, Anthinium, Chaetomium, Diaporthe, Nigrospora, one to family Xylariaceae and one was unclassified. All fungal isolates showed some inhibitory action over th...
Source: Microbiological Research - December 13, 2015 Category: Infectious Diseases Source Type: research

Biochemical and molecular tools reveal two diverse Xanthomonas groups in bananas
In this study, gram-negative and yellow-pigmented mucoid bacteria were isolated from BXW symptomatic and symptomless bananas collected from different parts of Uganda. Biolog, Xcm-specific (GspDm), Xanthomonas vasicola species-specific (NZ085) and Xanthomonas genus-specific (X1623) primers in PCR, and sequencing of ITS region were used to identify and characterize the isolates. Biolog tests revealed several isolates as xanthomonads. The GspDm and NZ085 primers accurately identified three isolates from diseased bananas as Xcm and these were pathogenic when re-inoculated into bananas. DNA from more isolates than those amplifi...
Source: Microbiological Research - December 10, 2015 Category: Infectious Diseases Source Type: research

Immunodiagnosis and molecular validation of Toxoplasma gondii-recombinant dense granular (GRA) 7 protein for the detection of toxoplasmosis in patients with cancer
Publication date: February 2016 Source:Microbiological Research, Volume 183 Author(s): Zahra Arab-Mazar, Shirzad Fallahi, Ameneh Koochaki, Ali Haghighi, Seyyed Javad Seyyed Tabaei Serological assays for the diagnosis of toxoplasmosis mostly rely on the tachyzoite specific antigens of Toxoplasma gondii, which are difficult to produce by conventional methods. The aim of this study was to clone and express of GRA7 protein of T. gondii and evaluate its potential for immunodiagnosis of toxoplasmosis in cancer patients. As well as validate the results using a new molecular assay, LAMP technique. The GRA7 gene was successf...
Source: Microbiological Research - December 5, 2015 Category: Infectious Diseases Source Type: research

Role of phosphate solubilizing Burkholderia spp. for successful colonization and growth promotion of Lycopodium cernuum L. (Lycopodiaceae) in lateritic belt of Birbhum district of West Bengal, India
Publication date: Available online 2 December 2015 Source:Microbiological Research Author(s): Ranjan Ghosh, Soma Barman, Rajib Mukherjee, NarayanC. Mandal Profuse growth of Lycpodium cernuum L. was found in phosphate deficient red lateritic soil of West Bengal, India. Interaction of vesicular-arbuscular mycorrhiza (VAM) with Lycopodium rhizoids were described earlier but association of PGPR with their rhizoids were not studied. Three potent phosphate solubilizing bacterial strains (P4, P9 and P10) associated with L. cernuum rhizoids were isolated and identified by 16S rDNA homologies on Ez-Taxon database as Burkholde...
Source: Microbiological Research - December 2, 2015 Category: Infectious Diseases Source Type: research

Multifunctionality and diversity of culturable bacterial communities strictly associated with spores of the plant beneficial symbiont Rhizophagus intraradices
Publication date: Available online 2 December 2015 Source:Microbiological Research Author(s): Fabio Battini, Caterina Cristani, Manuela Giovannetti, Monica Agnolucci Arbuscular Mycorrhizal Fungi (AMF) live in symbiosis with most crop plants and represent essential elements of soil fertility and plant nutrition and productivity, facilitating soil mineral nutrient uptake and protecting plants from biotic and abiotic stresses. These beneficial services may be mediated by the dense and active spore-associated bacterial communities, which sustain diverse functions, such as the promotion of mycorrhizal activity, biological...
Source: Microbiological Research - December 2, 2015 Category: Infectious Diseases Source Type: research

Gibberellins in Penicillium strains: challenges for endophyte-plant host interactions under salinity stress
Publication date: Available online 1 December 2015 Source:Microbiological Research Author(s): Ana Lúcia Leitão, Francisco J. Enguita The genus Penicillium is one of the most versatile “mycofactories”, comprising some species able to produce gibberellins, bioactive compounds that can modulate plant growth and development. Although plants have the ability to synthesize gibberellins, their levels are lower when plants are under salinity stress. It has been recognized that detrimental abiotic conditions, such as saline stress, have negative effects on plants, being the availability of bioactive gi...
Source: Microbiological Research - December 1, 2015 Category: Infectious Diseases Source Type: research

Proteomic response of the phytopathogen Phyllosticta citricarpa to antimicrobial volatile organic compounds from Saccharomyces cerevisiae
Publication date: Available online 1 December 2015 Source:Microbiological Research Author(s): Mauricio Batista Fialho, Alexander de Andrade, José Matheus Camargo Bonatto, Fernanda Salvato, Carlos Alberto Labate, Sérgio Florentino Pascholati Volatile organic compounds (VOCs) released by Saccharomyces cerevisiae inhibit plant pathogens, including the filamentous fungus Phyllosticta citricarpa, causal agent of citrus black spot. VOCs mediate relevant interactions between organisms in nature, and antimicrobial VOCs are promising, environmentally safer fumigants to control phytopathogens. As the mechanisms...
Source: Microbiological Research - December 1, 2015 Category: Infectious Diseases Source Type: research

AdeR protein regulates adeABC expression by binding to a direct-repeat motif in the intercistronic spacer
Publication date: Available online 26 November 2015 Source:Microbiological Research Author(s): Tein-Yao Chang, Bo-Jun Huang, Jun-Ren Sun, Cherng-Lih Perng, Ming-Chin Chan, Cheng-Ping Yu, Tzong-Shi Chiueh Overexpression of the efflux pump AdeABC is associated with tigecycline resistance of multi-drug resistant Acinetobacter baumannii (MDRAB). A two-component regulatory system, sensor AdeS and regulator AdeR proteins regulate the pump. However, the detailed mechanism of the AdeR protein to enhance the expression of adeABC operon is not well defined. We illustrated the biological characteristics of AdeR proteins by c...
Source: Microbiological Research - November 27, 2015 Category: Infectious Diseases Source Type: research

Immunodiagnosis and molecular validation of Toxoplasma gondii -recombinant dense granular (GRA) 7 protein for the detection of toxoplasmosis in patients with cancer
Publication date: Available online 25 November 2015 Source:Microbiological Research Author(s): Zahra Arab-Mazar, Shirzad Fallahi, Amaneh Koochaki, Ali Haghighi, Seyyed Javad Seyyed-Tabaei Serological assays for the diagnosis of toxoplasmosis mostly rely on the tachyzoite specific antigens of Toxoplasma gondii, which are difficult to produce by conventional methods. The aim of this study was to clone and express of GRA7 protein of T. gondii and evaluate its potential for immunodiagnosis of toxoplasmosis in cancer patients. As well as validate the results using a new molecular assay, LAMP technique. The GRA7 gene was ...
Source: Microbiological Research - November 25, 2015 Category: Infectious Diseases Source Type: research

Plant Growth-Promoting Bacterial Endophytes
Publication date: Available online 25 November 2015 Source:Microbiological Research Author(s): Gustavo Santoyo, Gabriel Moreno-Hagelsieb, Ma. del Carmen Orozco-Mosqueda, Bernard R. Glick Bacterial endophytes ubiquitously colonize the internal tissues of plants, being found in nearly every plant worldwide. Some endophytes are able to promote the growth of plants. For those strains the mechanisms of plant growth-promotion known to be employed by bacterial endophytes are similar to the mechanisms used by rhizospheric bacteria, e.g. the acquisition of resources needed for plant growth and modulation of plant growth and d...
Source: Microbiological Research - November 25, 2015 Category: Infectious Diseases Source Type: research

Bacteria and fungi can contribute to nutrients bioavailability and aggregate formation in degraded soils
Publication date: Available online 25 November 2015 Source:Microbiological Research Author(s): Muhammad Imtiaz Rashid, Liyakat Hamid Mujawar, Tanvir Shahzad, Talal Almeelbi, Iqbal M.I. Ismail, Mohammad Oves Intensive agricultural practices and cultivation of exhaustive crops has deteriorated soil fertility and its quality in agroecosystems. According to an estimate, such practices will convert 30% of the total world cultivated soil into degraded land by 2020. Soil structure or fertility loss are the main cause of soil degradation. They are also considered as a major threat to crop production and food security for f...
Source: Microbiological Research - November 25, 2015 Category: Infectious Diseases Source Type: research

Localization and Characterization of Two Putative TMH Family Proteins in Chlamydia psittaci
Publication date: Available online 25 November 2015 Source:Microbiological Research Author(s): Haiying Wu, Chuan Wang, Chuanhao Jiang, Yafeng Xie, Liangzhuan Liu, Ying Song, Xiaohua Ma, Yimou Wu Chlamydia psittaci (C. psittaci), an obligate intracellular agent of psittacosis, causes an atypical pneumonia in humans. The transmembrane head proteins (TMH) of C. psittaci, putatively belong to the Inc family and presumably play similar roles. CPSIT_0844 and CPSIT_0846 were the putativeTMH proteins of C. psittaci. To identify these two proteinsantisera were raised with fusion proteins which were prokaryotic expressed i...
Source: Microbiological Research - November 25, 2015 Category: Infectious Diseases Source Type: research

Diversity of bacterial endophytes in 3 and 15 year-old grapevines of Vitis vinifera cv. Corvina and their potential for plant growth promotion and phytopathogen control
This study represents the first investigation on ecology of endophytic bacteria isolated from 3 and 15 year-old vine stems of Vitis vinifera cv. Corvina. The analysis was performed by means of culture-dependent techniques. The obtained results showed that new grapevine endophytic genera are being discovered. Moreover, Bacilli and Actinobacteria are frequently isolated from 3 year-old plants, whereas Alfa- and Gamma– Proteobacteria classes are more prevalent in the 15 year-old plants. Shannon–Wiener (H) index and analysis of rarefaction curves revealed greater genus richness in young grapevine plants. Furthermor...
Source: Microbiological Research - November 25, 2015 Category: Infectious Diseases Source Type: research

Penicillium chrysogenum DSOA associated with marine sponge (Tedania anhelans) exhibit antimycobacterial activity
Publication date: Available online 23 November 2015 Source:Microbiological Research Author(s): Visamsetti Amarendra, Ramachandran Sarojini Santhosh, Kandasamy Dhevendaran A strain of Penicillium chrysogenum was isolated from Tedania anhelans (marine sponge) collected from Indian Ocean (8° 22′ 30″ N latitude and 76° 59′ 16″ longitude) and deposited in culture collection centers. The strain subjected to different culture conditions for production of extrolites were extracted using ethyl acetate and chloroform. When both extracts were subjected for antibacterial activity, latter had high a...
Source: Microbiological Research - November 24, 2015 Category: Infectious Diseases Source Type: research

Strain-level diversity of secondary metabolism in the biocontrol species Aneurinibacillus migulanus
Publication date: January 2016 Source:Microbiological Research, Volume 182 Author(s): Faizah N. Alenezi, Imen Rekik, Marta Bełka, Abrar F. Ibrahim, Lenka Luptakova, Marcel Jaspars, Steve Woodward, Lassaad Belbahri Aneurinibacillus migulanus strains Nagano and NCTC 7096 show potential in biocontrol against fungal and fungus-like plant pathogens, including a wide range of Ascomycota, Basidiomycota, and Oomycetes. Differences in terms of the range of pathogens that each strain inhibits, however, suggested that production of a single antibiotic cyclic peptide, gramicidin S (GS), by the two strains, is not the sole m...
Source: Microbiological Research - November 5, 2015 Category: Infectious Diseases Source Type: research

Transcriptomic analysis of the white rot fungus Polyporus brumalis provides insight into sesquiterpene biosynthesis
Publication date: Available online 30 October 2015 Source:Microbiological Research Author(s): Su-Yeon Lee, Myungkil Kim, Seon-Hong Kim, Chang-Young Hong, Sun-Hwa Ryu, In-Gyu Choi Object of this study was to identify genes and enzymes that are involved in sesquiterpene biosynthesis in the wood rotting fungus, Polyporus brumalis. Sesquiterpenes, β-eudesmane and β-eudesmol, were produced by the mycelium of P. brumalis cultured in modified medium. However, theses final products were not observed when the fungus was grown in potato dextrose medium. We used next generation sequencing (NGS) to identify different...
Source: Microbiological Research - November 1, 2015 Category: Infectious Diseases Source Type: research

Strain-level diversity of secondary metabolism in the biocontrol species Aneurinibacillus migulanus.
Publication date: Available online 28 October 2015 Source:Microbiological Research Author(s): Faizah N. Alenezi, Imen Rekik, Marta Bełka, Abrar F. Ibrahim, Lenka Luptakova, Marcel Jaspars, Steve Woodward, Lassaad Belbahri Aneurinibacillus migulanus strains Nagano and NCTC 7096 show potential in biocontrol against fungal and fungus-like plant pathogens, including a wide range of Ascomycota, Basidiomycota, and Oomycetes. Differences in terms of the range of pathogens that each strain inhibits, however, suggested that production of a single antibiotic cyclic peptide, gramicidin S (GS), by the two strains, is not th...
Source: Microbiological Research - October 29, 2015 Category: Infectious Diseases Source Type: research

Modulation of genetic clusters for synthesis of bioactive molecules in fungal endophytes: A review
Publication date: Available online 29 October 2015 Source:Microbiological Research Author(s): V.B. Deepika, T.S. Murali, K. Satyamoorthy Novel drugs with unique and targeted mode of action are very much need of the hour to treat and manage severe multidrug infections and other life-threatening complications. Though natural molecules have proved to be effective and environmentally safe, the relative paucity of discovery of new drugs has forced us to lean towards synthetic chemistry for developing novel drug molecules. Plants and microbes are the major resources that we rely upon in our pursuit towards discovery of nove...
Source: Microbiological Research - October 29, 2015 Category: Infectious Diseases Source Type: research

Metabolic potential and community structure of endophytic and rhizosphere bacteria associated with the roots of the halophyte Aster tripolium L.
Publication date: January 2016 Source:Microbiological Research, Volume 182 Author(s): Sonia Szymańska, Tomasz Płociniczak, Zofia Piotrowska-Seget, Michał Złoch, Silke Ruppel, Katarzyna Hrynkiewicz The submitted work assumes that the abundance and diversity of endophytic and rhizosphere microorganisms co-existing with the halophytic plant Aster tripolium L. growing in a salty meadow in the vicinity of a soda factory (central Poland) represent unique populations of cultivable bacterial strains. Endophytic and rhizosphere bacteria were (i) isolated and identified based on 16S rDNA sequences; (ii) screened for nifH...
Source: Microbiological Research - October 29, 2015 Category: Infectious Diseases Source Type: research

Microbial terroir and food innovation: the case of yeast biodiversity in wine
Publication date: Available online 19 October 2015 Source:Microbiological Research Author(s): Vittorio Capozzi, Carmela Garofalo, Maria Assunta Chiriatti, Francesco Grieco, Giuseppe Spano Saccharomyces and non-Saccharomyces represents a heterogeneous class in the grape/must/wine environments including several yeast genera (e.g. Saccharomyces, Hanseniaspora, Pichia, Candida, Metschnikowia, Kluyveromyces, Zygosaccharomyces, Torulaspora, Dekkera and Schizosaccharomyces) and species. Since each species may differently contribute to the improvement/depreciation of wine qualities, it appears clear the reason why species b...
Source: Microbiological Research - October 26, 2015 Category: Infectious Diseases Source Type: research

Long lasting effects of the conversion from natural forest to poplar plantation on soil microbial communities
In this study, we evaluate the long-lasting effects on soil microbial communities of a change within a single land-use category, specifically the conversion from natural forest to forest plantation. To minimize the effects of impacts other than land-use (i.e., climatic and anthropogenic), we chose three sites within a Natural Park, with homogeneous orographic and soil texture characteristics. We compared microbial diversity in a total of 156 soil samples from two natural mixed forests and a similar forest converted to poplar plantation about thirty years ago. The diversity and structure of bacterial and fungal communities ...
Source: Microbiological Research - October 26, 2015 Category: Infectious Diseases Source Type: research

Bacterial diversity indicates dietary overlap among bats of different feeding habits
Publication date: Available online 21 October 2015 Source:Microbiological Research Author(s): Sunil Banskar, D.T. Mourya, Yogesh S. Shouche Bats are among the most conspicuous mammals with extra ordinary adaptations. They play a key role in the ecosystem. Frugivorous bats are important seed dispersing agents that help in maintaining forest tree diversity, while insectivorous bats are natural insect pest control agents. Several previous reports suggest that bats are reservoir of viruses; nonetheless their bacterial counterparts are relatively less explored. The present study describes the microbial diversity associated...
Source: Microbiological Research - October 26, 2015 Category: Infectious Diseases Source Type: research

The tight-adhesion proteins TadGEF of Bradyrhizobium diazoefficiens USDA 110 are involved in cell adhesion and infectivity on soybean roots
Publication date: Available online 23 October 2015 Source:Microbiological Research Author(s): Elías J. Mongiardini, Gustavo D. Parisi, Juan I. Quelas, Aníbal R. Lodeiro Adhesion of symbiotic bacteria to host plants is an essential early step of the infection process that leads to the beneficial interaction. In the Bradyrhizobium diazoefficiens -soybean symbiosis few molecular determinants of adhesion are known. Here we identified the tight-adhesion gene products TadGEF in the open-reading frames blr3941-blr3943 of the Bradyrhizobium diazoefficiens USDA 110 complete genomic sequence. Predicted structure ...
Source: Microbiological Research - October 26, 2015 Category: Infectious Diseases Source Type: research

Biochemical and functional characterization of the periplasmic domain of the outer membrane protein A from enterohemorrhagic Escherichia coli
Publication date: Available online 23 October 2015 Source:Microbiological Research Author(s): Haiguang Wang, Qian Li, Yao Fang, Shu Yu, Bin Tang, Li Na, Bo Yu, Quanming Zou, Xuhu Mao, Jiang Gu Outer membrane protein A (OmpA) plays multiple roles in the physiology and pathogenesis of the zoonotic pathogen enterohemorrhagic Escherichia coli (EHEC). The N-terminus of OmpA forms a transmembrane domain (OmpA™), and the roles of this domain in bacterial pathogenesis have been well studied. However, how its C-terminal domain (OmpAper), which is located at the periplasmic space in the bacterial membrane, contribu...
Source: Microbiological Research - October 26, 2015 Category: Infectious Diseases Source Type: research

Comparative analysis of the gastrointestinal microbial communities of bar-headed goose (Anser indicus) in different breeding patterns by high-throughput sequencing
Publication date: Available online 23 October 2015 Source:Microbiological Research Author(s): Wen Wang, Jian Cao, Ji-Rong Li, Fang Yang, Zhuo Li, Lai-Xing Li The bar-headed goose is currently one of the most popular species for rare birds breeding in China. However, bar-headed geese in captivity display a reduced reproductive rate. The gut microbiome has been shown to influence host factors such as nutrient and energy metabolism, immune homeostasis and reproduction. It is therefore of great scientific and agriculture value to analyze the microbial communities associated with bar-headed geese in order to improve the...
Source: Microbiological Research - October 26, 2015 Category: Infectious Diseases Source Type: research

Agrobacterium tumefaciens-mediated transformation: an efficient tool for insertional mutagenesis and targeted gene disruption in Harpophora oryzae
In this study, Agrobacterium tumefaciens-mediated transformation (ATMT) was employed and optimized to modify the H. oryzae genes by either random DNA fragment integration or targeted gene replacement. Our results showed that co-cultivation of H. oryzae conidia with A. tumefaciens in the presence of acetosyringone for 48h at 22°C could lead to a relatively highest frequency of transformation, and 200μM acetosyringone (AS) pre-cultivation of A. tumefaciens is also suggested. ATMT-mediated knockout mutagenesis was accomplished with the gene-deletion cassettes using a yeast homologous recombination method with a yeast-E...
Source: Microbiological Research - October 10, 2015 Category: Infectious Diseases Source Type: research

Mycosynthesis of silver and gold nanoparticles: optimization, characterization and antimicrobial activity against human pathogens
This study was aimed to isolate soil fungi from Kolli and Yercaud Hills, South India with the ultimate objective of producing antimicrobial nanoparticles. Among 65 fungi tested, the isolate, Bios PTK 6 extracellularly synthesized both silver and gold nanoparticles with good monodispersity. Under optimized reaction conditions, the strain Bios PTK 6 identified as Aspergillus terreus has produced extremely stable nanoparticles within 12h. These nanoparticles were characterized by UV-Vis. spectrophotometer, HR-TEM, FTIR, XRD, EDX, SAED, ICP-AES and Zetasizer analyses. A. terreus synthesized 8-20nm sized, spherical shaped silve...
Source: Microbiological Research - October 4, 2015 Category: Infectious Diseases Source Type: research

Antagonistic effects of Bacillus subtilis subsp. subtilis and B. amyloliquefaciens against Macrophomina phaseolina: SEM study of fungal changes and UV-MALDI-TOF MS analysis of their bioactive compounds
Publication date: Available online 30 September 2015 Source:Microbiological Research Author(s): M.J. Torres, C. Pérez Brandan, G. Petroselli, R. Erra-Balsells, M.C. Audisio The antifungal effect of Bacillus subtilis subsp. subtilis PGPMori7 and B. amyloliquefaciens PGPBacCA1 was evaluated against Macrophomina phaseolina (Tassi) Goid. Cell suspension (CS), cell-free supernatant (CFS) and the lipopeptide fraction (LF) of PGPMori7 and PGPBacCA1 were screened against three different M. phaseolina strains. CS exhibited the highest inhibitory effect (around 50%) when compared to those of CFS and LF, regardless of t...
Source: Microbiological Research - October 2, 2015 Category: Infectious Diseases Source Type: research

Characterization of bacterial diversity associated with calcareous deposits and drip-waters, and isolation of calcifying bacteria from two Colombian mines
In this study, conventional microbiological methods and molecular tools, such as temporal temperature gradient electrophoresis (TTGE), were used to assess the composition of bacterial communities associated with carbonate deposits and drip-waters from two Colombian mines. A genetic analysis of these bacterial communities revealed a similar level of diversity, based on the number of bands detected using TTGE. The dominant phylogenetic affiliations of the bacteria, determined using 16S rRNA gene sequencing, were grouped into two phyla: Proteobacteria and Firmicutes. Within these phyla, seven genera were capable of precipitat...
Source: Microbiological Research - October 2, 2015 Category: Infectious Diseases Source Type: research

Characterization of an extracellular biofunctional alginate lyase from marine Microbulbifer sp. ALW1 and antioxidant activity of enzymatic hydrolysates
Publication date: Available online 25 September 2015 Source:Microbiological Research Author(s): Yanbing Zhu, Liyun Wu, Yanhong Chen, Hui Ni, Anfeng Xiao, Huinong Cai A novel alginate-degrading marine bacterium Microbulbifer sp. ALW1 was isolated from rotten brown alga. An extracellular alginate lyase was purified to electrophoretic homogeneity and had a molecular mass of about 26.0 kDa determined by SDS-PAGE and size exclusion chromatography. This enzyme showed activities towards both polyguluronate and polymannuronate indicating its bifunctionality while with preference for the former substrate. Using sodium algin...
Source: Microbiological Research - September 26, 2015 Category: Infectious Diseases Source Type: research

Residue mutations of xylanase in Aspergillus kawachii alter its optimum pH
In this study, we synthesized the A. kawachii xylanase gene (XynC) on the basis of the bias codon of yeast and mutated the gene in the dominating region related to optimum pH shifting during gene synthesis. After the overexpression of this gene in Pichia pastoris G115, the mutant (Thr64Ser) enzyme (XynC-C) showed an optimum pH of 3.8, which indicated partial alkalinity compared with the original xylanase from A. kawachii. Similar to that of the enzyme with one residue mutation (Asp48Asn), the optimum pH of the enzyme with two residue mutations (Thr64Ser and Asp48Asn) shifted to 5.0. The result indicated that mutation Asp48...
Source: Microbiological Research - September 12, 2015 Category: Infectious Diseases Source Type: research

Activities of Aureobasidium pullulans cell filtrates against Monilinia laxa of peaches
Publication date: Available online 10 September 2015 Source:Microbiological Research Author(s): Alessandra Di Francesco, Roberta Roberti, Camilla Martini, Elena Baraldi, Marta Mari The Aureobasidium pullulans L1 and L8 strains are known as efficient biocontrol agents against several postharvest fungal pathogens. In order to better understand the mechanism of action underneath the antifungal activity of L1 and L8 strains, yeast cell filtrates grown at different times were evaluated in vivo against Monilinia laxa on peach. Lesion diameters on peach fruit were reduced by L1 and L8 culture filtrates of 42.5% and 67 % re...
Source: Microbiological Research - September 12, 2015 Category: Infectious Diseases Source Type: research

Acid tolerance response of Bordetella bronchiseptica in avirulent phase
Publication date: Available online 5 September 2015 Source:Microbiological Research Author(s): M. Fingermann, D. Hozbor Bordetella bronchiseptica is a Gram-negative bacterium responsible for respiratory diseases in many mammalian hosts, including humans. This pathogen has been shown as able to persist inside the host cells, even in the phagosomes that are acidified to pH 4.5 to 5.0 after bacterial infection. Here we evaluated the resistance of B. bronchiseptica to survive under acidic conditions. In particular we analyzed the bacterial capacity to develop the mechanism known as acid tolerance response (ATR). Our studie...
Source: Microbiological Research - September 6, 2015 Category: Infectious Diseases Source Type: research