Transcriptome sequencing analysis of novel sRNAs of Kineococcus radiotolerans in response to ionizing radiation
In this study, the raw data of radiation-exposed samples (RS) and control samples (CS) were acquired separately from the sequencing platform. There were 217 common sRNA candidates in the two samples screened in the genome-wide scale by bioinformatics analysis. There were 43 differentially expressed sRNA candidates, including 28 up-regulated and 15 down-regulated ones. The down-regulated sRNAs were selected for the sRNA target prediction, of which 12 sRNAs that may modulate the genes related to the transcription regulation and DNA repair were considered as the candidates involved in the radio-resistance regulation system. (...
Source: Microbiological Research - August 30, 2016 Category: Infectious Diseases Source Type: research

Indentification of vincamine indole alkaloids producing endophytic fungi isolated from Nerium indicum, Apocynaceae
In conclusion, the fungal endophytes from Nerium indicum can be used as alternative source for the production of vincamine and vincamine analogues. (Source: Microbiological Research)
Source: Microbiological Research - August 30, 2016 Category: Infectious Diseases Source Type: research

Volatile organic compounds produced by Pseudomonas fluorescens WR-1 restrict the growth and virulence traits of Ralstonia solanacearum
This study revealed the significance of VOCs of P. fluorescens WR-1 to control the tomato wilt pathogen R. solanacearum. Investigation of the modes of action of biocontrol agents is important to better comprehend the interactions mediated by VOCs in nature to design better control strategies for plant pathogens. Graphical abstract (Source: Microbiological Research)
Source: Microbiological Research - August 30, 2016 Category: Infectious Diseases Source Type: research

Overexpression of a pathway specific negative regulator enhances production of daunorubicin in bldA deficient Streptomyces peucetius ATCC 27952
Publication date: November 2016 Source:Microbiological Research, Volume 192 Author(s): Anaya Raj Pokhrel, Amit Kumar Chaudhary, Hue Thi Nguyen, Dipesh Dhakal, Tuoi Thi Le, Anil Shrestha, Kwangkyoung Liou, Jae Kyung Sohng The dnrO gene is the first regulator to be activated in the daunorubicin (DNR) biosynthesis pathway of Streptomyces peucetius ATCC 27952. DnrO is known for its self-repression capability while it activates rest of the DNR biosynthesis pathway through cascades of regulatory events. S. peucetius was found to contain no functional copy of bldA-tRNA while a detailed examination of dnrO codons reveals the pres...
Source: Microbiological Research - August 30, 2016 Category: Infectious Diseases Source Type: research

Do cultural conditions induce differential protein expression: Profiling of extracellular proteome of Aspergillus terreus CM20
Publication date: November 2016 Source:Microbiological Research, Volume 192 Author(s): Saritha M., Surender Singh, Rameshwar Tiwari, Renu Goel, Lata Nain The present study reports the diversity in extracellular proteins expressed by the filamentous fungus, Aspergillus terreus CM20 with respect to differential hydrolytic enzyme production profiles in submerged fermentation (SmF) and solid-state fermentation (SSF) conditions, and analysis of the extracellular proteome. The SSF method was superior in terms of increase in enzyme activities resulting in 1.5–3 fold enhancement as compared to SmF, which was explained by th...
Source: Microbiological Research - August 30, 2016 Category: Infectious Diseases Source Type: research

Bacillus velezensis RC 218 as a biocontrol agent to reduce Fusarium head blight and deoxynivalenol accumulation: Genome sequencing and secondary metabolite cluster profiles
This study represents the first confirmed production of ericin by a B. velezensis strain. The genome also allowed us to do a comparative genomics with its closest relatives and compare the secondary metabolite production of the publically available B. velezensis genomes. The results showed that the diversity in secondary metabolites of strains in the B. velezensis clade is driven by strains making different antibacterials. Graphical abstract (Source: Microbiological Research)
Source: Microbiological Research - August 30, 2016 Category: Infectious Diseases Source Type: research

Yucat án in black and red: Linking edaphic analysis and pyrosequencing-based assessment of bacterial and fungal community structures in the two main kinds of soil of Yucatán State
Publication date: July–August 2016 Source:Microbiological Research, Volumes 188–189 Author(s): Héctor Estrada-Medina, Blondy B. Canto-Canché, César De los Santos-Briones, Aileen O’Connor-Sánchez Yucatán State is dominated by two kinds of soil, named “Black Leptosol” and “Red Leptosol”, which are interwoven across the State. In this work, we analyzed the relation between the edaphic characteristics and the bacterial and fungal community structures in these two kinds of Leptosol. The results revealed that Black Leptosol (BlaS) had a higher con...
Source: Microbiological Research - June 17, 2016 Category: Infectious Diseases Source Type: research

Significance of Hyphae Formation in Virulence of Candida tropicalis and Transcriptomic Analysis of Hyphal Cells
This study reports several virulence factors, including biofilm and hyphae formation, proteinase, phospholipase, lipase and hemolytic activity, in 52 clinical isolates of C. tropicalis collected from five hospitals in four provinces of China. Some C. tropicalis tended to produce more hyphae than others in the same circumstance. Six C. tropicalis strains with different morphologies were injected into mice via the tail vein, and the survival proportions and fungal burdens of the strains were evaluated. Hyphal production by C. tropicalis was associated with stronger virulence. RNA sequencing revealed that C. tropicalis with m...
Source: Microbiological Research - June 17, 2016 Category: Infectious Diseases Source Type: research

Role of ppGpp in Pseudomonas aeruginosa acute pulmonary infection and virulence regulation
Publication date: Available online 15 June 2016 Source:Microbiological Research Author(s): Xiaohui Xu, Hua Yu, Di Zhang, Junzhi Xiong, Jing Qiu, Rong Xin, Xiaomei He, Halei Sheng, Wenqiang Cai, Lu Jiang, Kebin Zhang, Xiaomei Hu During infection, bacteria might generate adaptive responses to facilitate their survival and colonization in the host environment. The alarmone guanosine 5′-triphosphate-3′-diphosphate (ppGpp), the levels of which are regulated by the RelA and SpoT enzymes, plays a critical role in mediating bacterial adaptive responses and virulence. However, the mechanism by which ppGpp ...
Source: Microbiological Research - June 14, 2016 Category: Infectious Diseases Source Type: research

Phytogenic synthesis of silver nanoparticles, optimization and evaluation of In vitro antifungal activity against human and plant pathogens
Publication date: Available online 8 June 2016 Source:Microbiological Research Author(s): P. Balashanmugam, M.D. Balakumaran, R. Murugan, K. Dhanapal, P.T. Kalaichelvan An attempt was made to synthesis of biocompatible silver nanoparticles from ten different Cassia sp. Among them, C. roxburghii aqueous leaf extract supported the synthesis of highly efficient and stable AgNPs. The synthesis of AgNPs was optimized at different physico-chemical condition and highly stable AgNPs were synthesized with 1. 0mL of C. roxburghii leaf extract, pH 7.0, 1.0mM AgNO3 and at 37°C. The synthesized AgNPs were characterized by XP...
Source: Microbiological Research - June 8, 2016 Category: Infectious Diseases Source Type: research

Adenylyl cyclase is required for cAMP production, growth, conidial germination, and virulence in the citrus green mold pathogen Penicillium digitatum
Publication date: Available online 3 June 2016 Source:Microbiological Research Author(s): Weili Wang, Mingshuang Wang, Jiye Wang, Congyi Zhu, Kuang-Ren Chung, Hongye Li Penicillium digitatum is the causative agent of green mold decay on citrus fruit. The cAMP-mediated signaling pathway plays an important role in the transduction of extracellular signals and has been shown to regulate a wide range of developmental processes and pathogenicity in fungal pathogens. We cloned and characterized a Pdac1 gene of P. digitatum, which encodes a polypeptide similar to fungal adenylyl cyclases. Using a loss-of-function mutation...
Source: Microbiological Research - June 2, 2016 Category: Infectious Diseases Source Type: research

Endophytic and rhizosphere bacteria associated with the roots of the halophyte Salicornia europaea L. – community structure and metabolic potential
Publication date: Available online 3 June 2016 Source:Microbiological Research Author(s): Sonia Szymańska, Tomasz Płociniczak, Zofia Piotrowska-Seget, Katarzyna Hrynkiewicz The main objective of our study was to assess density and diversity of rhizosphere (R) and endophytic (E) microorganisms associated with the halophyte S. europaea. Microorganisms were isolated from two saline sites (S1: 55dSm−1, anthropogenic origin; S2: 112dSm−1, natural salinity) located in central Poland and compared with microbial populations in the soil (S) using culture-independent (phospholipid fatty acids analysis, PLFA) and ...
Source: Microbiological Research - June 2, 2016 Category: Infectious Diseases Source Type: research

Differential expression of pectolytic enzyme genes in Xanthomonas citri subsp. citri and demonstration that pectate lyase Pel3 is required for the formation of citrus canker
In this study, the promoter activity of the pectolytic enzyme genes pel1, pel2, pel3, pglA, and peh-1 were investigated in Xcc XW19 strain using the β-glucuronidase (GUS) gene as a reporter. GUS activity expressed under the control of the pel1, pel3, pglA, and peh-1 gene promoters positively correlated with bacterial growth. These gene promoters displayed high GUS activity in the presence of sodium polypectate. In addition, the four genes were induced in XVM2 minimal medium. However, only pel1 was subjected to catabolite repression by glucose. GUS activity was significantly enhanced in the XW19-derived reporter strain...
Source: Microbiological Research - May 27, 2016 Category: Infectious Diseases Source Type: research

A predicted T4 secretion system and conserved DNA-repeats identified in a subset of related Arthrobacter plasmids
Publication date: Available online 24 May 2016 Source:Microbiological Research Author(s): Marius Mihasan, Roderich Brandsch BLAST analysis of pAO1 ORFs of Arthrobacter nicotinovorans revealed 12 ORFs, including the ORF of a transcriptional regulator, predicted to encode the components of a T4-secretion system involved in bacterial conjugation. These ORFs were conserved and showed synteny among 14 Arthrobacter plasmids. A DNA repeat of about 370 nucleotides was found to be present 5′ to the pAO1 ORFs of DUF4192-, DprA- and ParB-like proteins. Similar repeats were present in identical positions on 12 additional Art...
Source: Microbiological Research - May 23, 2016 Category: Infectious Diseases Source Type: research

In vitro and in vivo antibiofilm potential of 2,4-Di-tert-butylphenol from seaweed surface associated bacterium Bacillus subtilis against Group A Streptococcus
Publication date: Available online 21 May 2016 Source:Microbiological Research Author(s): Dharmaprakash Viszwapriya, Udayakumar Prithika, Sundaresan Deebika, Krishnaswamy Balamurugan, Shunmugiah Karutha Pandian Biofilm formation of Group A Streptococcus (GAS) is recognized as an important virulent determinant. The present study reports the antibiofilm potential of seaweed (Gracilaria gracilis) surface associated Bacillus subtilis against GAS. Purification revealed 2,4-Di-tert-butyl-phenol (DTBP) as the active principle. DTBP exhibited a dose dependent antibiofilm activity against GAS (SF370 & six different c...
Source: Microbiological Research - May 21, 2016 Category: Infectious Diseases Source Type: research

DNA barcoding and isolation of vertically transmitted ascomycetes in sorghum from Burkina Faso: Epicoccum sorghinum is dominant in seedlings and appears as a common root pathogen
This study provides the first molecular approach to characterize the seedling mycoflora of sorghum in Western Africa and suggests E. sorghinum as a common root pathogen. (Source: Microbiological Research)
Source: Microbiological Research - May 20, 2016 Category: Infectious Diseases Source Type: research

Extracellular Expression and Antiviral Activity of a Bovine Interferon-Alpha through Codon Optimization in Pichia pastoris
In this study, we demonstrated high-level extracellular expression of opti-bIFN-α by P. pastoris. To the best of our knowledge, the opti-bIFN-α yield observed in this study is the highest to be reported to date. Our results demonstrated that the extracellular opti-bIFN-α with strong antiviral activity could be easily prepared and purified at a low cost and that it may be a potential biological therapeutic drug against bovine viral infections. (Source: Microbiological Research)
Source: Microbiological Research - May 19, 2016 Category: Infectious Diseases Source Type: research

(p)ppGpp synthetases regulate the pathogenesis of zoonotic Streptococcus suis
Publication date: Available online 19 May 2016 Source:Microbiological Research Author(s): Jiawen Zhu, Tengfei Zhang, Zhipeng Su, Lu Li, Dong Wang, Ran Xiao, Muye Teng, Meifang Tan, Rui Zhou (p)ppGpp-mediated stringent response is one of the main adaption mechanism in bacteria, and the ability to adapt to environment is linked to the pathogenesis of bacterial pathogens. In the zoonotic pathogen Streptococcus suis, there are two (p)ppGpp synthetases, RelA and RelQ. To investigate the regulatory functions of (p)ppGpp/(p)ppGpp synthetases on the pathogenesis of S. suis, the phenotypes of the [(p)ppGpp00] mutant &Del...
Source: Microbiological Research - May 19, 2016 Category: Infectious Diseases Source Type: research

Phosphatidylcholine Affects the Secretion of the Alkaline Phosphatase PhoA in Pseudomonas Strains
Publication date: Available online 20 May 2016 Source:Microbiological Research Author(s): Xin Liu, Deliang Long, Heng You, Dingpeng Yang, Shuang Zhou, Shuting Zhang, Mengqiu Li, Miao He, Ming Xiong, Xingguo Wang Pseudomonas aeruginosa ATCC 27853 and Pseudomonas sp. 593 use the phosphatidylcholine synthase pathway (Pcs-pathway) for the biosynthesis of phosphatidylcholine (PC). Both bacterial strains contain the phoA and lapA genes encoding alkaline phosphatases (ALP) and display strong ALP activities. The PhoA and LapA enzymes are thought to be independently secreted via the Xcp and Hxc type II secretion system ...
Source: Microbiological Research - May 19, 2016 Category: Infectious Diseases Source Type: research

In Azospirillum brasilense, mutations in flmA or flmB genes affect polar flagellum assembly, surface polysaccharides, and attachment to maize roots
Publication date: Available online 14 May 2016 Source:Microbiological Research Author(s): Fernando Ariel Rossi, Daniela Beatriz Medeot, Juan Pablo Liaudat, Mariano Pistorio, Edgardo Jofré Azospirillum brasilense is a soil bacterium capable of promoting plant growth. Several surface components were previously reported to be involved in the attachment of A. brasilense to root plants. Among these components are the exopolysaccharide (EPS), lipopolysaccharide (LPS) and the polar flagellum. Flagellin from polar flagellum is glycosylated and it was suggested that genes involved in such a posttranslational modificat...
Source: Microbiological Research - May 14, 2016 Category: Infectious Diseases Source Type: research

Phenol degradation by Sulfobacillus acidophilus TPY via the meta-pathway
Publication date: Available online 14 May 2016 Source:Microbiological Research Author(s): Wengen Zhou, Wenbin Guo, Hongbo Zhou, Xinhua Chen Due to its toxicity and volatility, phenol must be cleared from the environment. Sulfobacillus acidophilus TPY, which was isolated from a hydrothermal vent in the Pacific Ocean as a moderately thermoacidophilic Gram-positive bacterium, was capable of aerobically degrading phenol. This bacterium could tolerate up to 1,300mg/L phenol and degrade 100mg/L phenol in 40h completely at 45°C and pH 1.8 with a maximal degradation rate of 2.32mg/L/h at 38h. Genome-wide search revealed ...
Source: Microbiological Research - May 14, 2016 Category: Infectious Diseases Source Type: research

Bacteria as growth-promoting agents for citrus rootstocks
Publication date: Available online 14 May 2016 Source:Microbiological Research Author(s): Valdionei Giassi, Camila Kiritani, Katia Cristina Kupper The microbial community plays an essential role in maintaining the ecological balance of soils. Interactions between microorganisms and plants have a major influence on the nutrition and health of the latter, and growth-promoting rhizobacteria can be used to improve plant development through a wide range of mechanisms. Therefore, the objective of the present study was to evaluate bacteria as growth-promoting agents for citrus rootstocks. A total of 30 bacterial isolates (11...
Source: Microbiological Research - May 13, 2016 Category: Infectious Diseases Source Type: research

Binding of a proline-independent hydrophobic motif by the Candida albicans Rvs167-3 SH3 domain
Publication date: Available online 10 May 2016 Source:Microbiological Research Author(s): Areti Gkourtsa, Janny van den Burg, Teja Avula, Frans Hochstenbach, Ben Distel Src-homology 3 (SH3) domains are small protein-protein interaction modules. While most SH3 domains bind to proline-x-x-proline (PxxP) containing motifs in their binding partners, some SH3 domains recognize motifs other than proline-based sequences. Recently, we showed that the SH3 domain of C. albicans Rvs167-3 binds peptides enriched in hydrophobic residues and containing a single proline residue (RΦxΦxΦP, where x is any amino acid and &...
Source: Microbiological Research - May 10, 2016 Category: Infectious Diseases Source Type: research

A phenazine-1-carboxylic acid producing polyextremophilic Pseudomonas chlororaphis (MCC2693) strain, isolated from mountain ecosystem, possesses biocontrol and plant growth promotion abilities
Publication date: Available online 10 May 2016 Source:Microbiological Research Author(s): Rahul Jain, Anita Pandey The genus Pseudomonas is known to comprise a huge diversity of species with the ability to thrive in different habitats, including those considered as extreme environments. In the present study, a psychrotolerant, wide pH tolerant and halotolerant strain of Pseudomonas chlororaphis GBPI_507 (MCC2693), isolated from the wheat rhizosphere growing in a mountain location in Indian Himalayan Region, has been investigated for its antimicrobial potential with particular reference to phenazine production and plant...
Source: Microbiological Research - May 10, 2016 Category: Infectious Diseases Source Type: research

Illumina-based analysis of core actinobacteriome in roots, stems, and grains of rice
Publication date: Available online 10 May 2016 Source:Microbiological Research Author(s): Wenfeng Wang, Yanyan Zhai, Lixiang Cao, Hongming Tan, Renduo Zhang Seed-borne microbiota can transmit vertically from generation to generation and be a favour mutualism between the endosymbionts and hosts. The aim of this study was to investigate the rice-associated actinobacterial taxa in roots, stems, and grains and explore vertically transmitted core actinobacteriome of rice plants. Illumina sequencing analyses of samples of rice grains, stems, and roots showed that the roots contained the most diverse actinobacteria among t...
Source: Microbiological Research - May 9, 2016 Category: Infectious Diseases Source Type: research

Evaluation of acrylamide-removing properties of two Lactobacillus strains under simulated gastrointestinal conditions using a dynamic system
Publication date: Available online 10 May 2016 Source:Microbiological Research Author(s): L. Rivas-Jimenez, K. Ramírez-Ortiz, A.F. González-Córdova, B. Vallejo-Cordoba, H.S. Garcia, A. Hernandez-Mendoza The aim of this study was to evaluate the capability of Lactobacillus reuteri NRRL 14171 and Lactobacillus casei Shirota to remove dietary acrylamide (AA) under simulated gastrointestinal conditions using a dynamic system. The effects of different AA levels or bacteria concentration on toxin removal by Lactobacillus strains were assessed. Thereafter, AA-removing capability of bacteria strains un...
Source: Microbiological Research - May 9, 2016 Category: Infectious Diseases Source Type: research

Alternatives to overcoming bacterial resistances: state-of-the-art
Publication date: Available online 7 May 2016 Source:Microbiological Research Author(s): Alessandra C. Rios, Carla G. Moutinho, Flávio C. Pinto, Fernando S. Del Fiol, Angela Jozala, Marco V. Chaud, Marta M.D.C. Vila, José A. Teixeira, Victor M. Balcão Worldwide, bacterial resistance to chemical antibiotics has reached such a high level that endangers public health. Presently, the adoption of alternative strategies that promote the elimination of resistant microbial strains from the environment is of utmost importance. This review discusses and analyses several (potential) alternative strateg...
Source: Microbiological Research - May 7, 2016 Category: Infectious Diseases Source Type: research

A Serine/threonine Kinase PknL, is involved in the adaptive response of Mycobacterium tuberculosis
In this study, we have investigated the role of PknL, a STPK in the adaptive responses of M. tuberculosis by conditional inactivation of the gene using antisense technology. The inhibition of PknL in the knockdown strain was validated by RT-PCR. The in vitro growth kinetics of M. tuberculosis strain following inhibition of PknL was found to be bacteriostatic. The knock down strain of PknL exhibited a better survival in pH 5.5 when compared to its growth in pH 7.0. Similarly, it also exhibited more resistance to both SDS(0.01%) and Lysozyme stress (2.5mg/ml), indicating that loss of PknL enhances the growth of mycobacterium...
Source: Microbiological Research - May 7, 2016 Category: Infectious Diseases Source Type: research

2,4-Dichlorophenoxyacetic acid (2,4-D)- and 2,4,5-trichlorophenoxyacetic acid (2,4,5-T)-degrading gene cluster in the soybean root-nodulating bacterium Bradyrhizobium elkanii USDA94
In this study, we identified cadRABCK in the genome of a type strain of soybean root-nodulating B. elkanii USDA94 and demonstrated that the strain could degrade the herbicides when cadABCK was forcibly expressed. cadABCK-cloned Escherichia coli also showed the degrading ability. Because co-spiked phenoxyacetic acid (PAA) could induce the degradation of 2,4-D in B. elkanii USDA94, the lack of degrading ability in this strain was supposed to be due to the low inducing potential of the herbicides for the degrading gene cluster. On the other hand, tfdAα from B. elkanii USDA94 showed little potential to degrade the herbic...
Source: Microbiological Research - May 5, 2016 Category: Infectious Diseases Source Type: research

Oligoribonuclease is required for the type III secretion system and pathogenesis of Pseudomonas aeruginosa
Publication date: Available online 4 May 2016 Source:Microbiological Research Author(s): Gukui Chen, Qiang Zhao, Feng Zhu, Ronghao Chen, Yongxin Jin, Chang Liu, Xiaolei Pan, Shouguang Jin, Weihui Wu, Zhihui Cheng Oligoribonuclease (Orn) is a 3′ to 5′ exonuclease that degrades nanoRNAs, which can serve as primers for transcription initiation at a significant fraction of promoters. One of Orn’s substrates, pGpG inhibits the enzymatic activity of EAL-domain containing phosphodiesterases (PDEs), thereby increasing intracellular cyclic-di-GMP (c-di-GMP) level. Here, we found that an orn mutant of P...
Source: Microbiological Research - May 4, 2016 Category: Infectious Diseases Source Type: research

Assessing the effects of heavy metals in ACC deaminase and IAA production on plant growth-promoting bacteria
This study poses a methodology in order to simultaneously quantify ACC deaminase and IAA levels in the same culture medium. Ten bacterial strains isolated from plant rhizosphere naturally settled in mining residues were chosen. These bacterial strains were characterized as PGPB, and all of them showed at least three characteristics (indole-3 acetic acid and siderophore production, ACC deaminase enzyme activity, and inorganic phosphate solubilization). Taxonomic identification showed that the strains belong to Enterobacter, Serratia, Klebsiella, and Escherichia genera. Similarly, both the ACC deaminase enzyme activity and t...
Source: Microbiological Research - May 4, 2016 Category: Infectious Diseases Source Type: research

Investigation of Archaeal and Bacterial community structure of five different small drinking water networks with special regard to the nitrifying microorganisms
Publication date: Available online 4 May 2016 Source:Microbiological Research Author(s): Zsuzsanna Nagymáté, Zalán G. Homonnay, Károly Márialigeti Total microbial community structure, and particularly nitrifying communities inhabiting five different small drinking water networks characterized with different water physical and chemical parameters was investigated, using cultivation-based methods and sequence aided Terminal Restriction Fragment Length Polymorphism (T-RFLP) analysis. Ammonium ion, originated from well water, was only partially oxidized via nitrite to nitrate in the drin...
Source: Microbiological Research - May 4, 2016 Category: Infectious Diseases Source Type: research

Role of Vfr in the regulation of antifungal compound production by Pseudomonas fluorescens FD6
This study investigated the effect of a vfr mutant derived from P. fluorescens FD6 to better understand the regulation of some important biocontrol traits associated with the bacterium. Biochemical studies indicated that the production of the antibiotics 2,4-diacetylphloroglucinol, pyrrolnitrin and pyoluteorin, was markedly enhanced in the vfr mutant. The vfr mutation also increased biofilm production, swimming motility and the expression of exopolysaccharide-associated gene (pelA, pslA and pslB) transcripts, but reduced protease production. Wheat rhizosphere and root tip colonization by the vfr mutant was higher than that...
Source: Microbiological Research - May 2, 2016 Category: Infectious Diseases Source Type: research

Biocontrol ability and putative mode of action of yeasts against Geotrichum citri-aurantii in citrus fruit
Publication date: Available online 28 April 2016 Source:Microbiological Research Author(s): Luriany Pompeo Ferraz, Tatiane da Cunha, Aline Caroline da Silva, Katia Cristina Kupper Sour rot is a major postharvest disease of citrus fruit and is caused by the fungal pathogen Geotrichum citri-aurantii. A lack of chemicals certified for the control of this disease has led to the consideration of alternative methods and strategies, such as the use of yeasts as biocontrol agents. The purpose of the present study was to test the ability of yeasts isolated from leaves, flowers, fruit, and soil, and six Saccharomyces cerevisia...
Source: Microbiological Research - April 28, 2016 Category: Infectious Diseases Source Type: research

Biochemical properties and crystal structure of the flavin reductase FerA from Paracoccus denitrificans
Publication date: Available online 27 April 2016 Source:Microbiological Research Author(s): Vojtěch Sedláček, Tomáš Klumpler, Jaromír Marek, Igor Kučera The Pden_2689 gene encoding FerA, an NADH:flavin oxidoreductase required for growth of P. denitrificans under iron limitation, was cloned and overexpressed as a C-terminally His6-tagged derivative. The binding of substrates and products was detected and quantified by isothermal titration calorimetry and fluorometric titration. FerA binds FMN and FAD with comparable affinity in an enthalpically driven, entropically opposed process. The r...
Source: Microbiological Research - April 27, 2016 Category: Infectious Diseases Source Type: research

Actinomycetes from Eucalyptus and their biological activities for controlling Eucalyptus leaf and shoot blight
In this study, a total of 477 actinomycete strains were successfully isolated from roots and rhizosphere soil of Eucalyptus. Four hundred and thirty nine isolates were classified as streptomycetes and 38 isolates were non-streptomycetes. Among these isolates, 272 (57.0%), 118 (24.7%) and 241 (50.5%) isolates were antagonistic to Cryptosporiopsis eucalypti, Cylindrocladium sp. and Teratosphaeria destructans, respectively. All isolates were tested for their abilities to produce siderophores, indole acetic acid (IAA) and solubilise phosphate. Most isolates (464, 97.3%) produced siderophores. The majority of isolates (345, 72....
Source: Microbiological Research - April 27, 2016 Category: Infectious Diseases Source Type: research

Inhibition of multidrug resistant Listeria monocytogenes by peptides isolated from combinatorial phage display libraries
Publication date: Available online 27 April 2016 Source:Microbiological Research Author(s): Z. Flachbartova, L. Pulzova, E. Bencurova, L. Potocnakova, L. Comor, Z. Bednarikova, M. Bhide The aim of the study was to isolate and characterize novel antimicrobial peptides from peptide phage library with antimicrobial activity against multidrug resistant Listeria monocytogenes. Combinatorial phage-display library was used to affinity select peptides binding to the cell surface of multidrug resistant L. monocytogenes. After several rounds of affinity selection followed by sequencing, three peptides were revealed as the m...
Source: Microbiological Research - April 27, 2016 Category: Infectious Diseases Source Type: research

Endophytic bacterial and fungal microbiota in sprouts, roots and stems of rice (Oryza sativa L.)
The objective of this study was to elucidate the endophytic microbiota in rice sprouts, roots, and stems, and their transmission in the plant development. Prior to DNA extraction, roots and stems were treated with 36% formaldehyde and 0.1M NaOH solutions to remove epiphytic bacterial whole 16S rRNA genes. Bacterial and fungal taxa in the sprout, root, and stem samples were analyzed using Illumina-based sequencing of the V3-V4 hyper variable regions of bacterial 16S rRNA genes and the ITS2 regions of fungal rRNA genes, respectively. Results showed that more diverse bacterial OTUs were detected in roots than in stems, while ...
Source: Microbiological Research - April 22, 2016 Category: Infectious Diseases Source Type: research

Yucatán in black and red: Linking edaphic analysis and pyrosequencing-based assessment of bacterial and fungal community structures in the two main kinds of soil of Yucatán State
Publication date: Available online 22 April 2016 Source:Microbiological Research Author(s): Héctor Estrada-Medina, Blondy B. Canto-Canché, César De los Santos-Briones, Aileen O’Connor-Sánchez Yucatán State is dominated by two kinds of soil, named “Black Leptosol” and “Red Leptosol”, which are interwoven across the State. In this work, we analyzed the relation between the edaphic characteristics and the bacterial and fungal community structures in these two kinds of Leptosol. The results revealed that Black Leptosol (BlaS) had a higher content of calcium...
Source: Microbiological Research - April 22, 2016 Category: Infectious Diseases Source Type: research

Diaporthe endophytica and D. terebinthifolii from medicinal plants for biological control of Phyllosticta citricarpa
Publication date: Available online 9 April 2016 Source:Microbiological Research Author(s): Paulo José Camargo Dos Santos, Daiani Cristina Savi, Renata Rodrigues Gomes, Eduardo Henrique Goulin, Camila Da Costa Senkiv, Francisco André Ossamu Tanaka, Álvaro Manuel Rodrigues Almeida, Lygia Galli-Terasawa, Vanessa Kava, Chirlei Glienke The citrus industry is severely affected by citrus black spot (CBS), a disease caused by the pathogen Phyllosticta citricarpa. This disease causes loss of production, decrease in the market price of the fruit, and reduction in its export to the European Union. Cur...
Source: Microbiological Research - April 12, 2016 Category: Infectious Diseases Source Type: research

Roles of Two RyhB Paralogs in the Physiology of Salmonella enterica
Publication date: Available online 13 April 2016 Source:Microbiological Research Author(s): Jeong Nam Kim Salmonella has evolved complicated regulatory systems to regulate the expression of virulence determinants that are acquired by horizontal gene transfer in response to various environmental niches. Among these, sRNA-mediated regulation exhibits unique features, distinct from those of protein factor-mediated regulation, which may provide benefits for a pathogen coping with the complex stress conditions encountered during host infection. Specifically, iron acquisition by this pathogenic bacterium is important for cell...
Source: Microbiological Research - April 12, 2016 Category: Infectious Diseases Source Type: research

Enhancement of plant growth and yields in Chickpea (Cicer arietinum L.) through novel cyanobacterial and biofilmed inoculants
Publication date: Available online 13 April 2016 Source:Microbiological Research Author(s): Ngangom Bidyarani, Radha Prasanna, Santosh Babu, Firoz Hossain, Anil Kumar Saxena The use of Rhizobium inoculants in chickpea is well established; however, meagre efforts have been directed towards the use of other microbial supplements for improving nutrient uptake and yields. A set of novel cyanobacterial and biofilmed inoculants were evaluated in chickpea under field conditions. A significant two-fold enhancement in leghaemoglobin content of nodules and plant biomass was recorded with Anabaena laxa treatment. The inoculant...
Source: Microbiological Research - April 12, 2016 Category: Infectious Diseases Source Type: research

Characterization and antimicrobial potential of extremely halophilic archaea isolated from hypersaline environments of the Algerian Sahara
Publication date: Available online 9 April 2016 Source:Microbiological Research Author(s): Inès Quadri, Imene Ikrame Hassani, Stéphane l’Haridon, Morgane Chalopin, Hocine Hacène, Mohamed Jebbar Halophilic archaea were isolated from different chotts and sebkha, dry salt lakes and salt flat respectively, of the Algerian Sahara and characterized using phenotypic and phylogenetic approaches. From 102 extremely halophilic strains isolated, forty three were selected and studied. These strains were also screened for their antagonistic potential and the production of hydrolytic enzymes. Sequencin...
Source: Microbiological Research - April 8, 2016 Category: Infectious Diseases Source Type: research

A cost effective cultivation medium for biocalcification of Bacillus pasteurii KCTC 3558 and its effect on cement cubes properties
This study indicated that the effluent from chicken manure bio-gas plant could be used as an alternative cost effective culture medium for cultivation and biocalcification of B. pasteurii KCTC3558 in cement. (Source: Microbiological Research)
Source: Microbiological Research - April 5, 2016 Category: Infectious Diseases Source Type: research

Powered by light: Phototrophy and photosynthesis in prokaryotes and its evolution
Publication date: Available online 2 April 2016 Source:Microbiological Research Author(s): Beatrycze Nowicka, Jerzy Kruk Photosynthesis is a complex metabolic process enabling photosynthetic organisms to use solar energy for the reduction of carbon dioxide into biomass. This ancient pathway has revolutionized life on Earth. The most important event was the development of oxygenic photosynthesis. It had a tremendous impact on the Earth’s geochemistry and the evolution of living beings, as the rise of atmospheric molecular oxygen enabled the development of a highly efficient aerobic metabolism, which later led to t...
Source: Microbiological Research - April 2, 2016 Category: Infectious Diseases Source Type: research

Functions of Saccharomyces cerevisiae Ecm27p, a putative Na+/Ca2+ exchanger, in calcium homeostasis, carbohydrate storage and cell cycle reentry from the quiescent phase
Publication date: Available online 1 April 2016 Source:Microbiological Research Author(s): Rachel Klukovich, William E. Courchesne The quiescent phase of the cell cycle is of fundamental importance for fungi, yet our understanding of this phase of the cycle is much less well understood than the mitotic cell cycle. We found that the ECM27 gene, which encodes a Na+/Ca2+ exchanger, is responsible for influx of calcium from the extracellular space and release from intracellular stores during membrane stress. Wild type cells increase total Ca2+ in quiescence but cells lacking ECM27 gene fail to do so and are defective in ce...
Source: Microbiological Research - April 1, 2016 Category: Infectious Diseases Source Type: research

Diversity, ecological distribution and biotechnological potential of Actinobacteria inhabiting seamounts and non-seamounts in the Tyrrhenian Sea
Publication date: Available online 1 April 2016 Source:Microbiological Research Author(s): Besma Ettoumi, Habib Chouchane, Amel Guesmi, Mouna Mahjoubi, Lorenzo Brusetti, Mohamed Neifar, Sara Borin, Daniele Daffonchio, Ameur Cherif In the present study, the ecological distribution of marine Actinobacteria isolated from seamount and non-seamount stations in the Tyrrhenian Sea was investigated. A collection of 110 isolates was analyzed by Automated Ribosomal Intergenic Spacer Analysis (ARISA) and 16S rRNA gene sequencing of representatives for each ARISA haplotype (n=49). Phylogenetic analysis of 16S rRNA sequences...
Source: Microbiological Research - April 1, 2016 Category: Infectious Diseases Source Type: research

The effect of mutation in the clpX gene on the synthesis of N-acyl-homoserine lactones and other properties of Burkholderia cenocepacia 370
Publication date: Available online 1 April 2016 Source:Microbiological Research Author(s): M.A. Veselova, Yu. M. Romanova, V.A. Lipasova, O.A. Koksharova, Yu. V. Zaitseva, M.U. Chernukha, A.L. Gintsburg, I.A. Khmel In order to study the regulation of N-acyl-homoserine lactones synthesis (AHLs, the signal molecules of Quorum Sensing regulation) in Burkholderia cenocepacia strain 370 we obtained mutants with increased AHL production. One of the mutants, named BC-B6, was obtained by TnMod-RKmr plasposon mutagenesis. The plasposon insertion was located within the clpX gene encoding the ATPase subunit ClpX of the ClpX...
Source: Microbiological Research - April 1, 2016 Category: Infectious Diseases Source Type: research

The antibacterial activity and action mechanism of emodin from Polygonum cuspidatum against Haemophilus parasuis in vitro
In this study, emodin from Polygonum cuspidatum showed potent inhibitory effect against H. parasuis. The minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) values of emodin were 32 and 64μg/mL, respectively. The antibacterial kinetic curves indicated the antibacterial activity of emodin was in a concentration-dependent manner. Cell membrane permeability and flow cytometry assays proved that emodin could destroy cell membrane integrity and increase membrane permeability, and fluorescence spectra assay indicated emodin has influenced conformation of membrane protein. Under transmission ele...
Source: Microbiological Research - April 1, 2016 Category: Infectious Diseases Source Type: research

Discovering novel enzymes by Functional screening of plurigenomic libraries from alga-associated Flavobacteriia and Gammaproteobacteria
Publication date: Available online 24 March 2016 Source:Microbiological Research Author(s): Marjolaine Martin, Marie Vandermies, Coline Joyeux, Renée Martin, Tristan Barbeyron, Gurvan Michel, Micheline Vandenbol Alga-associated microorganisms, in the context of their numerous interactions with the host and the complexity of the marine environment, are known to produce diverse hydrolytic enzymes with original biochemistry. We recently isolated several macroalgal-polysaccharide-degrading bacteria from the surface of the brown alga Ascophyllum nodosum. These active isolates belong to two classes: the Flavobact...
Source: Microbiological Research - March 24, 2016 Category: Infectious Diseases Source Type: research