The opportunistic human fungal pathogen Candida albicans promotes the growth and proliferation of commensal Escherichia coli through an iron-responsive pathway
In this study, we investigated the interactions between C. albicans and the bacterium Escherichia coli and unexpectedly observed that C. albicans enhances growth and proliferation of Escherichia coli strain K12 by facilitating its cell division. Importantly, we found, based on our genetic screens, that both fungus- and bacterium-derived factors, including the iron-responsive transcription factors Sef1 and Sfu1 in C. albicans and the siderophere enterobactin transporters FepD and FepG in E. coli, actively contribute to this transkingdom interaction. Deletion of SFU1 or SEF1 caused a dramatic reduction in growth enhancement ...
Source: Microbiological Research - December 21, 2017 Category: Infectious Diseases Source Type: research

Could FlhF be a key element that controls Campylobacter jejuni flagella biosynthesis in the initial assembly stage?
In this study, we found that inactivation of FlhF caused the transcription of most flagella genes down-regulated. The importance of FlhF was systematically evaluated by analyzing changes in the transcription profiles between wild-type and flhF mutant strains, which showed that FlhF affects late flagella genes obviously. FlhF is constitutively expressed during C. jejuni growth, demonstrating that it is a class I flagella element that participates in early flagella assembly. In addition, the early flagella component FlhB was not localized to the cell pole in the flhF mutant. Thus, flagella assembly was impeded at the initial...
Source: Microbiological Research - December 20, 2017 Category: Infectious Diseases Source Type: research

First report of a cross-kingdom pathogenic bacterium, Achromobacter xylosoxidans isolated from stipe-rot Coprinus comatus
In this study, the pathogenic bacterium JTG-B1, identified as Achromobacter xylosoxidans by 16S rDNA and nrdA gene sequencing, was isolated from edible mushroom Coprinus comatus with serious rot disease on its stipe. A. xylosoxidans has been confirmed as an important opportunistic human pathogenic bacterium and has been isolated from respiratory samples from cystic fibrosis. It is widely distributed in the environment. Here, we first report that fungi can also serve as a host for A. xylosoxidans. We confirmed that it can cross-kingdom infect between animals (mice) and fungi (C. comatus). The results of pathogenicity tests,...
Source: Microbiological Research - December 20, 2017 Category: Infectious Diseases Source Type: research

Monitoring biocalcification potential of Lysinibacillus sp. isolated from alluvial soils for improved compressive strength of concrete
Publication date: Available online 18 December 2017 Source:Microbiological Research Author(s): Rajneesh Vashisht, Sampan Attri, Deepak Sharma, Abhilash Shukla, Gunjan Goel The present study reports the potential of newly isolated calcite precipitating bacteria isolated from alluvial soil to improve the strength and durability of concrete. A total of sixteen samples of alluvial soil and sewage were collected from the different locations of province Solan (India). For isolation, enrichment culture technique was used to enrich calcite precipitating strains in Urea broth. After enrichment, fourteen distinct bacterial strains ...
Source: Microbiological Research - December 19, 2017 Category: Infectious Diseases Source Type: research

Fusarium species as pathogen on orchids
Publication date: Available online 7 December 2017 Source:Microbiological Research Author(s): Shikha Srivastava, Chris Kadooka, Janice Y. Uchida The recent surge in demand for exotic ornamental crops such as orchids has led to a rise in international production, and a sharp increase in the number of plant and plant products moving between countries. Along with the plants, diseases are also being transported and introduced into new areas. Fusarium is one of the major diseases causing pathogens infecting orchids that is spreading through international trade. Studies have identified several species of Fusarium associated wit...
Source: Microbiological Research - December 9, 2017 Category: Infectious Diseases Source Type: research

Evaluation of fungal community involved in the bioderioration process of wooden artworks and canvases in Montefeltro area (Marche, Italy)
This study could be useful to better understand the microorganism-related phenomena in cultural heritage of Marche region, identifying the potential risks and defining preventive protecting such as climate control, frequent cleaning and environmental monitoring. (Source: Microbiological Research)
Source: Microbiological Research - December 9, 2017 Category: Infectious Diseases Source Type: research

Revealing the inhibitory potential of Yersinia enterocolitica on cysteine proteases of the papain family
Publication date: Available online 8 December 2017 Source:Microbiological Research Author(s): Mateusz Kędzior, Aleksandra Pawlak, Rafał Seredyński, Jacek Bania, Aleksandra Platt-Samoraj, Magdalena Czemplik, Elżbieta Klausa, Gabriela Bugla-Płoskońska, Jan Gutowicz Cysteine proteases of the papain family, including mammalian cathepsins, play important physiological roles, however, their excessive activity may contribute to the development of various pathologies. Therefore, cysteine cathepsin inhibitors are being considered as promising drugs to treat cathepsin-driven diseases. Diverse saprophytic and parasitic microbe...
Source: Microbiological Research - December 9, 2017 Category: Infectious Diseases Source Type: research

Streptomyces puniceus strain AS13. Production, characterization and evaluation of bioactive metabolites: a new face of dinactin as an antitumor antibiotic
Publication date: Available online 8 December 2017 Source:Microbiological Research Author(s): Aehtesham Hussain, Muzafar Ahmad Rather, Mohd Saleem Dar, N.A. Dangroo, Mushtaq A. Aga, Arem qayum, Aabid Manzoor Shah, Zahoor Ahmad, Mohd Jamal Dar, Qazi Parvaiz Hassan A highly active actinobacterial strain isolated from untapped areas of Northwestern Himalayas and characterised as Streptomyces puniceus strain AS13 by 16S rRNA gene sequencing was selected for production of bioactive metabolites. The bioassay-guided fractionation of microbial cultured ethyl acetate extract of the strain, led to isolation of macrotetrolide compou...
Source: Microbiological Research - December 9, 2017 Category: Infectious Diseases Source Type: research

Combination of multilocus sequence typing and pulsed-field gel electrophoresis reveals an association of molecular clonality with the emergence of extensive-drug resistance (XDR) in Salmonella
Publication date: Available online 5 December 2017 Source:Microbiological Research Author(s): Yongzhong Cao, Yongxiu Shen, Lingling Cheng, Xiaorong Zhang, Chao Wang, Yan Wang, Xiaohui Zhou, Guoxiang Chao, Yantao Wu Salmonellae is one of the most important foodborne pathogens and becomes resistant to multiple antibiotics, which represents a significant challenge to food industry and public health. However, a molecular signature that can be used to distinguish antimicrobial resistance profile, particularly multi-drug resistance or extensive-drug resistance (XDR). In the current study, 168 isolates from the chicken and pork ...
Source: Microbiological Research - December 6, 2017 Category: Infectious Diseases Source Type: research

A glucanolytic Pseudomonas sp. associated with Smilax bona-nox L. displays strong activity against Phytophthora parasitica
Publication date: March 2018 Source:Microbiological Research, Volume 207 Author(s): Ashraf S.A. El-Sayed, Asma Akbar, Irum Iqrar, Robina Ali, David Norman, Mary Brennan, Gul Shad Ali Biological control is an eco-friendly strategy for mitigating and controlling plant diseases with negligible effects on human health and environment. Biocontrol agents are mostly isolated from field crops, and microbiomes associated with wild native plants is underexplored. The main objective of this study was to characterize the bacterial isolates associated with Smilax bona-nox L, a successful wild plant with invasive growth habits. Forty m...
Source: Microbiological Research - December 2, 2017 Category: Infectious Diseases Source Type: research

Leptospirosis in human: Biomarkers in host immune responses
Publication date: March 2018 Source:Microbiological Research, Volume 207 Author(s): Chin VK, Lee TY, Lim WF, Wan Shahriman YWY, Syafinaz AN, Zamberi S, Maha A Leptospirosis remains one of the most widespread zoonotic diseases caused by spirochetes of the genus Leptospira, which accounts for high morbidity and mortality globally. Leptospiral infections are often found in tropical and subtropical regions, with people exposed to contaminated environments or animal reservoirs are at high risk of getting the infection. Leptospirosis has a wide range of clinical manifestations with non-specific signs and symptoms and often misd...
Source: Microbiological Research - December 2, 2017 Category: Infectious Diseases Source Type: research

The special existences: nanoRNA and nanoRNase
Publication date: Available online 29 November 2017 Source:Microbiological Research Author(s): Hebin Liao, Mafeng Liu, Xiaolan Guo To adapt to a wide range of nutritional and environmental changes, cells must adjust their gene expression profiles. This process is completed by the frequent transcription and rapid degradation of mRNA. mRNA decay is initiated by a series of endo- and exoribonucleases. These enzymes leave behind 2- to 5-nt-long oligoribonucleotides termed “nanoRNAs” that are degraded by specific nanoRNases; the degradation of nanoRNA is essential because nanoRNA can mediate the priming of transcri...
Source: Microbiological Research - November 30, 2017 Category: Infectious Diseases Source Type: research

Modulation in phenolic root exudate profile of Abelmoschus esculentus expressing activation of defense pathway
In this study, shikimic, gallic, fumaric, ferulic, vanillic acid and quercetin in root exudates of Abelmoschus esculentus act as chemoattractants of endophytic Alcaligenes faecalis strains, BHU 12, BHU 16 and BHU M7. In vitro chemotaxis assay showed that BHU 12 expressed highest chemotactic movement (CFU ∼50×1012) towards A.esculentus root exudates followed by BHU 16 and BHU M7 (CFU∼ 9×1012), thereby confirming their ability to colonize the host rhizoplane region. However, BHU 16 expressed highest biofilm formation ability followed by BHU 12 and BHU M7. Assessment of chemotactic and biofilm formation po...
Source: Microbiological Research - November 30, 2017 Category: Infectious Diseases Source Type: research

Comparative analysis of gut bacterial communities of green turtles (Chelonia mydas) pre-hospitalization and post-rehabilitation by high-throughput sequencing of bacterial 16S rRNA gene
In this study, all post-rehabilitation green turtles exhibited similar bacterial communities, irrespective of their microbial compositions at pre-hospitalization. The marked differences in the gut bacterial communities of PH and PR turtles indicate the outcome of dietary, management and environmental shift during rehabilitation. Therefore, it is important to address the process of restoring normal gut microbiota of recovered turtles prior to release back to their natural habitat. (Source: Microbiological Research)
Source: Microbiological Research - November 30, 2017 Category: Infectious Diseases Source Type: research

A review on sustainable yeast biotechnological processes and applications
This study explores how different sustainable solutions used to overcome various environmental effects on yeast. This work targets a broad matrix of current advances and future prospect in yeast biotechnology and discusses their application and potential in general. (Source: Microbiological Research)
Source: Microbiological Research - November 30, 2017 Category: Infectious Diseases Source Type: research

Corrigendum to “Targets of ubiquitin like system in mycobacteria and related actinobacterial species” [Microbiol. Res. 204 (November 2017) 9–29]
Publication date: March 2018 Source:Microbiological Research, Volume 207 Author(s): Yusuf Akhter, Shweta Thakur (Source: Microbiological Research)
Source: Microbiological Research - November 30, 2017 Category: Infectious Diseases Source Type: research

Functional characterization of murB-potABCD operon for polyamine uptake and peptidoglycan synthesis in Streptococcus suis
This study reveals the functions of PotABCD in polyamine transport and the regulatory roles of polyamines in PG synthesis. Results provide new insights into the machineries contributing to normal growth and cell division of S. suis. (Source: Microbiological Research)
Source: Microbiological Research - November 15, 2017 Category: Infectious Diseases Source Type: research

DNA sequence-specific dimeric bisbenzimidazoles DBP(n) and DBPA(n) as inhibitors of H-NS silencing in bacterial cells
Publication date: Available online 14 November 2017 Source:Microbiological Research Author(s): Olga E. Melkina, Vasilii S. Koval, Alexander A. Ivanov, Alexei L. Zhuze, Gennadii B. Zavilgelsky DNA sequence-specific fluorescent dimeric bisbenzimidazoles DBP(n) and DBPA(n), noncovalently interacting with A-T pairs in the minor groove of double-stranded DNA were used for studying and monitoring the expression of histone-like H-NS-dependent promoters. Histone-like H-NS selectively binds to AT-rich segments of DNA and silences a large number of genes in bacterial chromosomes. The H-NS-dependent promoters of Quorum Sensing (QS)-...
Source: Microbiological Research - November 15, 2017 Category: Infectious Diseases Source Type: research

Screening of tropically derived, multi-trait plant growth- promoting rhizobacteria and evaluation of corn and soybean colonization ability
Publication date: January 2018 Source:Microbiological Research, Volume 206 Author(s): Bruna Durante Batista, Paulo Teixeira Lacava, Andrea Ferrari, Natalia Sousa Teixeira-Silva, Maria Letícia Bonatelli, Sarina Tsui, Mateus Mondin, Elliot Watanabe Kitajima, José Odair Pereira, João Lúcio Azevedo, Maria Carolina Quecine The present study assessed the plant growth-promoting (PGP) traits and diversity of culturable rhizobacteria associated with guarana (Paullinia cupana), a typical tropical plant. Ninety-six bacteria were isolated, subjected to biochemical tests, and identified by partial or total ...
Source: Microbiological Research - November 15, 2017 Category: Infectious Diseases Source Type: research

Stress response of a clinical Enterococcus faecalis isolate subjected to a novel antimicrobial surface coating
Publication date: Available online 12 November 2017 Source:Microbiological Research Author(s): Emanuel Clauss-Lendzian, Ankita Vaishampayan, Anne de Jong, Uwe Landau, Carsten Meyer, Jan Kok, Elisabeth Grohmann Emerging antibiotic resistance among pathogenic bacteria, paired with their ability to form biofilms on medical and technical devices, represents a serious problem for effective and long-term decontamination in health-care environments and gives rise to an urgent need for new antimicrobial materials. Here we present the impact of AGXX®, a novel broad-spectrum antimicrobial surface coating consisting of micro-gal...
Source: Microbiological Research - November 13, 2017 Category: Infectious Diseases Source Type: research

Rapid screening of starter cultures for maari based on antifungal properties
Publication date: Available online 11 November 2017 Source:Microbiological Research Author(s): Donatien Kaboré, Mérilie Gagnon, Denis Roy, Hagrétou Sawadogo-Lingani, Bréhima Diawara, Gisèle LaPointe Forty Bacillus isolates obtained from maari (used as condiment in Burkina Faso) including 17 B. subtilis, 4 B. circulans, 7 B. pumilus and 6 B. licheniformis were investigated for use as starter cultures in maari production. The isolates were screened by PCR for the sfp gene responsible for the production of the lipopeptide biosurfactant, surfactin. The sfp gene was detected in all of the sev...
Source: Microbiological Research - November 11, 2017 Category: Infectious Diseases Source Type: research

Does plant —Microbe interaction confer stress tolerance in plants: a review?
Publication date: Available online 9 November 2017 Source:Microbiological Research Author(s): Akhilesh Kumar, Jay Prakash Verma The biotic and abiotic stresses are major constraints for crop yield, food quality and global food security. A number of parameters such as physiological, biochemical, molecular of plants are affected under stress condition. Since the use of inorganic fertilizers and pesticides in agriculture practices cause degradation of soil fertility and environmental pollutions. Hence it is necessary to develop safer and sustainable means for agriculture production. The application of plant growth promoting ...
Source: Microbiological Research - November 9, 2017 Category: Infectious Diseases Source Type: research

Reactive oxygen species-independent apoptotic pathway by gold nanoparticles in Candida albicans
In conclusion, AuNPs induce ROS-independent apoptosis in C. albicans by causing DNA damage and mitochondria dysfunction. (Source: Microbiological Research)
Source: Microbiological Research - November 8, 2017 Category: Infectious Diseases Source Type: research

A critical role for very long-chain fatty acid elongases in oleic acid-mediated Saccharomyces cerevisiae cytotoxicity
Publication date: Available online 6 November 2017 Source:Microbiological Research Author(s): Qiao Wang, Xiuxiu Du, Ke Ma, Ping Shi, Wenbin Liu, Jing Sun, Min Peng, Zhiwei Huang Elongases FEN1/ELO2 and SUR4/ELO3 are important enzymes involved in the elongation of long-chain fatty acids (LCFAs) to very long-chain fatty acids (VLCFAs) in Saccharomyces cerevisiae. The molecular mechanism of the involvement of these elongases in lipotoxicity is unclear. In the present study, we investigated the role of VLCFA elongases in oleic acid-mediated yeast cytotoxicity. The spot test showed that yeast strains with the deletion of ELO2 ...
Source: Microbiological Research - November 8, 2017 Category: Infectious Diseases Source Type: research

5-hydroxymethyl-2-furaldehyde from marine bacterium Bacillus subtilis inhibits biofilm and virulence of Candida albicans
Publication date: Available online 8 November 2017 Source:Microbiological Research Author(s): Ganapathy Ashwinkumar Subramenium, Thirukannamangai Krishnan Swetha, Prasanth Mani Iyer, Krishnaswamy Balamurugan, Shunmugiah Karutha Pandian Candida albicans is considered as the primary etiologic agent of candidiasis, a very common fungal infection in human. The yeast to hyphal transition and ability to form hypoxic biofilm on medical devices is well allied with virulence and antifungal resistance of C. albicans. Antagonistic agents that inhibit biofilm formation and alter susceptibility of C. albicans to conventional antifunga...
Source: Microbiological Research - November 8, 2017 Category: Infectious Diseases Source Type: research

Synthesis, nature and utility of universal iron chelator – Siderophore: A review
Publication date: Available online 1 November 2017 Source:Microbiological Research Author(s): Azmi Khan, Pratika Singh, Amrita Srivastava Siderophores, the secondary metabolite of various microorganisms are ferric ion specific chelators secreted under iron stressed condition. These non-ribosomal peptides have been classified as catecholate, hydroxamate, carboxylate and mixed types. Recent studies focus on discovery of possible mammalian siderophores. The biosynthesis pathway including non-ribosomal dependent as well as non-ribosomal independent pathways are of great interest now a days. Many significant roles of sideropho...
Source: Microbiological Research - November 4, 2017 Category: Infectious Diseases Source Type: research

Synthesis, nature and utility of universal iron chelator − siderophore: a review
Publication date: Available online 1 November 2017 Source:Microbiological Research Author(s): Azmi Khan, Pratika Singh, Amrita Srivastava Siderophores, the secondary metabolite of various microorganisms are ferric ion specific chelators secreted under iron stressed condition. These non-ribosomal peptides have been classified as catecholate, hydroxamate, carboxylate and mixed types. Recent studies focus on discovery of possible mammalian siderophores. The biosynthesis pathway including non-ribosomal dependent as well as non-ribosomal independent pathways are of great interest now a days. Many significant roles of sideropho...
Source: Microbiological Research - November 3, 2017 Category: Infectious Diseases Source Type: research

Diversity of cultivable bacterial endophytes in Paullinia cupana and their potential for plant growth promotion and phytopathogen control
In this study, we addressed whether the plant genotype, organ, and geographic location influence the structure, composition, and functionality of endophytic bacterial communities in Paullinia cupana. To characterize the communities and identify strains with potential application in agriculture, we analyzed two P. cupana genotypes cultivated in two cities of the State of Amazonas, Brazil. Endophytic bacteria were isolated from surface-disinfested root, leaf, and seed tissues through the fragmentation and maceration techniques. The colonization rate, number of bacteria, richness, diversity, and functional traits were determi...
Source: Microbiological Research - November 1, 2017 Category: Infectious Diseases Source Type: research

Haemophilus parasuis CpxRA two-component system confers bacterial tolerance to environmental stresses and macrolide resistance
In this study, we investigated the role of TCST system CpxRA in H. parasuis in response to different environmental stimuli by constructing the ΔcpxA and ΔcpxR single deletion mutants as well as the ΔcpxRA double deletion mutant from H. parasuis serotype 4 isolate JS0135. We demonstrated that H. parasuis TCST system CpxRA confers bacterial tolerance to stresses and bactericidal antibiotics. The CpxR was found to play essential roles in mediating oxidative stress, osmotic stresses and alkaline pH stress tolerance, as well as macrolide resistance (i.e. erythromycin), but the CpxA deletion did not decrease ba...
Source: Microbiological Research - October 28, 2017 Category: Infectious Diseases Source Type: research

Isolation, purification, structural elucidation and antimicrobial activities of kocumarin, a novel antibiotic isolated from actinobacterium Kocuria marina CMG S2 associated with the brown seaweed Pelvetia canaliculata
Publication date: Available online 26 October 2017 Source:Microbiological Research Author(s): Bushra Uzair, Farid Menaa, Barkat Ali Khan, Faryal Vali Mohammad, Viqar Uddin Ahmad, Ryad Djeribi, Bouzid Menaa Aims Screening of seaweed-associated bacteria capable of producing antimicrobials. Methods and results Fifteen microbial strains, associated to the brown seaweed Pelvetia canaliculata (Linnaeus) attached to the rocks of Sonmiani Beach (Karachi, Pakistan), were screened. Crude extract filtrates of CMG S2 strain grew on Zobell marine agar (ZMA) had the most remarkable antimicrobial activity. Based on its phenotypic aspect...
Source: Microbiological Research - October 27, 2017 Category: Infectious Diseases Source Type: research

Intertwined control of the cell cycle and nucleocytoplasmic transport by the cyclin-dependent kinase Pho85 and RanGTPase Gsp1 in Saccharomyces cerevisiae
Publication date: Available online 26 October 2017 Source:Microbiological Research Author(s): Oriol Mirallas, Elisabet Ballega, Bàrbara Samper-Martín, Sergio García-Márquez, Reyes Carballar, Natalia Ricco, Javier Jiménez, Josep Clotet Deciphering the molecular mechanisms that connect cell cycle progression and nucleocytoplasmic transport is of particular interest: this intertwined relationship, once understood, may provide useful insight on the diseases resulting from the malfunction of these processes. In the present study we report on findings that indicate a biochemical connection bet...
Source: Microbiological Research - October 27, 2017 Category: Infectious Diseases Source Type: research

Differential protein-DNA contacts for activation and repression by ArgP, a LysR-type (LTTR) transcriptional regulator in Escherichia coli
Publication date: Available online 23 October 2017 Source:Microbiological Research Author(s): Phu Nguyen Le Minh, Cristina Velázquez Ruiz, Steven Vandermeeren, Pamella Abwoyo, Indra Bervoets, Daniel Charlier ArgP is a LysR-type transcriptional regulator (LTTR) that operates with two effector molecules, lysine and arginine, to differentially regulate gene expression. Effector-free ArgP stimulates transcription of all investigated regulon members, except argO, whereas lysine abolishes this effect. Activation of argO, encoding an exporter for arginine and canavanine, is strictly dependent on arginine-bound ArgP. Lysin...
Source: Microbiological Research - October 24, 2017 Category: Infectious Diseases Source Type: research

Composition of lactic acid bacteria during spontaneous curly kale (Brassica oleracea var. sabellica) fermentation
Publication date: Available online 16 October 2017 Source:Microbiological Research Author(s): Magdalena Michalak, Klaudia Gustaw, Adam Waśko, Magdalena Polak-Berecka The present work is the first report on spontaneous fermentation of curly kale and characteristics of autochthonous lactic acid bacteria (LAB). Our results indicate that curly kale fermentation is the new possibility of the technological use of this vegetable. Bacteria representing ten different species were isolated from three phases of curly kale fermentation and identified by MALDI-TOF mass spectrometry and 16S rRNA gene sequencing. Among them, four speci...
Source: Microbiological Research - October 22, 2017 Category: Infectious Diseases Source Type: research

The outer membrane protein OprF and the sigma factor SigX regulate antibiotic production in Pseudomonas fluorescens 2P24
In this study, we identified a novel role for the outer membrane protein gene oprF, in negatively regulating the 2,4-DAPG production by using random mini-Tn5 mutagentsis. A sigma factor gene sigX was located immediately upstream of the oprF gene and shown to be a positive regulator for oprF transcription and 2,4-DAPG production. Genetic analysis of an oprF and sigX double-mutant indicated that the 2,4-DAPG regulation by oprF was dependent on SigX. The sigX gene did not affect PhlA and PhlD expression, but positively regulated the level of malonyl-CoA, the substrate of 2,4-DAPG synthesis, by influencing the expression of ac...
Source: Microbiological Research - October 22, 2017 Category: Infectious Diseases Source Type: research

Revitalization of plant growth promoting rhizobacteria for sustainable development in agriculture
Publication date: Available online 17 October 2017 Source:Microbiological Research Author(s): Sushanto Gouda, Rout George Kerry, Gitishree Das, Spiros Paramithiotis, Han-Seung Shin, Jayanta Kumar Patra The progression of life in all forms is not only dependent on agricultural and food security but also on the soil characteristics. The dynamic nature of soil is a direct manifestation of soil microbes, bio-mineralization, and synergistic co-evolution with plants. With the increase in world’s population the demand for agriculture yield has increased tremendously and thereby leading to large scale production of chemical...
Source: Microbiological Research - October 22, 2017 Category: Infectious Diseases Source Type: research

Phosphatidylcholine absence affects the secretion of lipodepsipeptide phytoxins in Pseudomonas syringae pv. syringae van Hall CFCC 1336
In this study, we further analyzed virulence of lipodepsipeptide phytoxins of Pss 1336 wild type (pcs +), the 1336RM (pcs−/+) and the 1336 pcs− mutant, and found that the 1336 pcs− mutant was unable to cause necrosis of Chinese date fruits and inhibit fungal growth. HPLC analysis also showed that the 1336 pcs− mutant markedly reduced its secretion of lipodepsipeptide phytoxins. Analysis of semi-quantitative RT-PCR revealed that PC presence or absence did not affect gene expressions of SyrD, PseABC and PseEF efflux systems at transcriptional level. However, western blotting assays found that PseE and...
Source: Microbiological Research - October 22, 2017 Category: Infectious Diseases Source Type: research

Endophytism or saprophytism: Decoding the lifestyle transition of the generalist fungus Phomopsis liquidambari
Publication date: January 2018 Source:Microbiological Research, Volume 206 Author(s): Jun Zhou, Xin Li, Peng-Wei Huang, Chuan-Chao Dai Filamentous ascomycete Phomopsis sp. are common inhabitants of natural ecosystems and, as saprophytes, are largely responsible for the destructive decay of litterfall, promoting the carbon and nitrogen cycles. Phomopsis liquidambari B3 can establish mutualistic symbiosis with a broad spectrum of crop plants. Colonizing dynamics observations and a growth promotion assay of rice and Arabidopsis thaliana revealed that the B3 colonization strategy is host-adapted and resulted in different grow...
Source: Microbiological Research - October 22, 2017 Category: Infectious Diseases Source Type: research

SawR a new regulator controlling pyomelanin synthesis in Pseudomonas aeruginosa
In this study we describe how sawR regulates pyomelanin synthesis. We show that sawR down-regulates the expression levels of hmgA and this causes the accumulation of homogentisic acid which in turn undergoes oxidation and polymerization to pyomelanin. Using bioinformatics, we were able to identify a specific amino acid, arginine 23, which is found within the sawR DNA binding domain and is crucial for its regulatory activity. Our results indicate that sawR does not affect any other genes in the phenylalanine/tyrosine metabolic pathway and its repressive ability on hmgA is not mediated by the hmgA repressor PA2010 (i.e. hmgR...
Source: Microbiological Research - October 22, 2017 Category: Infectious Diseases Source Type: research

Transcription factors Atf1 and Sty1 promote stress tolerance under nitrosative stress in Schizosaccharomyces pombe
In this study, exposure to an NO donor resulted in S-phase slowdown with associated mitotic block in S. pombe. Deletion of sty1 and atf1 in S. pombe had differential growth sensitivity towards NO donor. Both Sty1 and Atf1 were involved in regulating mitotic slowdown in S. pombe under nitrosative stress. Experimental data obtained in this study reveals a novel role of Atf1 in initiating the replication slowdown in S. pombe under nitrosative stress. Both Sty1 and Atf1 were accumulated in the nucleus in S. pombe under nitrosative stress in a concentration and time dependent manner. Atf1 is also found to be nuclear delocalized...
Source: Microbiological Research - October 13, 2017 Category: Infectious Diseases Source Type: research

Pathogenic Labyrinthula associated with Australian seagrasses: Considerations for seagrass wasting disease in the southern hemisphere
Publication date: Available online 9 October 2017 Source:Microbiological Research Author(s): Stacey M. Trevathan-Tackett, Brooke K. Sullivan, Katie Robinson, Osu Lilje, Peter I. Macreadie, Frank H. Gleason Marine disease ecology is a growing field of research, particularly for host organisms negatively impacted by a changing climate and anthropogenic activities. A decrease in health and increase in susceptibility to disease has been hypothesised as the mechanism behind wide-spread seagrass die-offs related to wasting disease in the past. However, seagrass wasting disease and the causative pathogen, Labyrinthula, has been ...
Source: Microbiological Research - October 10, 2017 Category: Infectious Diseases Source Type: research

A comprehensive review of non-enterica subspecies of Salmonella enterica
Publication date: Available online 7 October 2017 Source:Microbiological Research Author(s): Alexandre Lamas, José Manuel Miranda, Patricia Regal, Beatriz Vázquez, Carlos Manuel Franco, Alberto Cepeda Salmonella is a major foodborne pathogen with a complex nomenclature. This genus is composed of two species, S. enterica and S. bongori. S. enterica is divided into six subspecies. S. enterica subspecies enterica is composed of more than 1,500 serotypes with some of great importance, such as S. Typhimurium and S. Enteritidis. S. enterica subsp. enterica is responsible of more than 99% of human salmonellosis and...
Source: Microbiological Research - October 8, 2017 Category: Infectious Diseases Source Type: research

Klebsiella sp. confers enhanced tolerance to salinity and plant growth promotion in oat seedlings (Avena sativa)
This study demonstrates that PGPR play an imperative function in stimulating salt tolerance in plants and can be used as biofertilizer to enhance growth of crops in saline areas. (Source: Microbiological Research)
Source: Microbiological Research - September 24, 2017 Category: Infectious Diseases Source Type: research

A novel Burkholderia ambifaria strain able to degrade the mycotoxin fusaric acid and to inhibit Fusarium spp. growth
Publication date: Available online 22 September 2017 Source:Microbiological Research Author(s): Ester Simonetti, Irma N. Roberts, Marcela S. Montecchia, Flavio H. Gutierrez-Boem, Federico M. Gomez, Jimena A. Ruiz Fusaric acid (FA) is a fungal metabolite produced by several Fusarium species responsible for wilts and root rot diseases of a great variety of plants. Bacillus spp. and Pseudomonas spp. have been considered as promising biocontrol agents against phytopathogenic Fusarium spp., however it has been demonstrated that FA negatively affects growth and production of some antibiotics in these bacteria. Thus, the capabil...
Source: Microbiological Research - September 23, 2017 Category: Infectious Diseases Source Type: research

Isolation and characterisation of theobromine-degrading filamentous fungi
Publication date: Available online 19 September 2017 Source:Microbiological Research Author(s): Daniel Oduro-Mensah, Augustine Ocloo, Sammy T. Lowor, Evelyn Y. Bonney, Laud K.N.A. Okine, Naa Ayikailey Adamafio Strategies for achieving global food security include identification of alternative feedstock for use as animal feed, to contribute towards efforts at increasing livestock farming. The presence of theobromine in cocoa pod husks, a major agro-waste in cocoa-producing countries, hinders its utilisation for this purpose. Cheap treatment of cocoa pod husks to remove theobromine would allow largescale beneficial use of t...
Source: Microbiological Research - September 20, 2017 Category: Infectious Diseases Source Type: research

Screening of Tropically Derived, Multi-Trait Plant Growth-Promoting Rhizobacteria and Evaluation of Corn and Soybean Colonization Ability
Publication date: Available online 20 September 2017 Source:Microbiological Research Author(s): Bruna Durante Batista, Paulo Teixeira Lacava, Andrea Ferrari, Natalia de Sousa Teixeira e Silva, Maria Letícia Bonatelli, Sarina Tsui, Mateus Mondin, Elliot Watanabe Kitajima, José Odair Pereira, João Lúcio Azevedo, Maria Carolina Quecine The present study assessed the plant growth-promoting (PGP) traits and diversity of culturable rhizobacteria associated with guarana (Paullinia cupana), a typical tropical plant. Ninety-six bacteria were isolated, subjected to biochemical tests, and identified by pa...
Source: Microbiological Research - September 20, 2017 Category: Infectious Diseases Source Type: research

Distribution of apple and blackcurrant microbiota in Lithuania and the Czech Republic
In this study, we report Next Generation Sequencing-based metagenomic analysis of microbial communities associated with apple and blackcurrant fruits harvested from Lithuania and the Czech Republic. Differences in the taxonomic composition of eukaryotic and prokaryotic microorganisms were observed between plant types. Our results revealed limited geographic differentiation between the bacterial and fungal communities associated with apples. In contrast, blackcurrant berries harvested from different regions demonstrated high diversity in both bacterial and fungal microbiota structures. Among fungal and bacterial microorgani...
Source: Microbiological Research - September 14, 2017 Category: Infectious Diseases Source Type: research

Combination of amplified rDNA restriction analysis and high-throughput sequencing revealed the negative effect of colistin sulfate on the diversity of soil microorganisms
In this study, we determined the effect of colistin sulfate on the diversity of soil microorganisms by amplified rDNA restriction analysis (ARDRA) and high-throughput sequencing.ARDRAshowed that the diversity of DNA from soil microorganisms was reduced after soil was treated with colistin sulfate, with the most dramatic reductionobserved after 35days of treatment. High-throughput sequencing showed that the Chao1 and abundance-based coverage estimators (ACE) were reduced in the soils treated with colistin sulfate for 35 dayscompared to those treated with colistin sulfate for 7 days. Furthermore, Chao1 and ACE tended to be l...
Source: Microbiological Research - September 14, 2017 Category: Infectious Diseases Source Type: research

Characterization and assessment of two biocontrol bacteria against Pseudomonas syringae wilt in Solanum lycopersicum and its genetic responses
Publication date: Available online 12 September 2017 Source:Microbiological Research Author(s): Kaliannan Durairaj, Palanivel Velmurugan, Jung-Hee Park, Woo-Suk Chang, Yool-Jin Park, Planinaicker Senthilkumar, Kyung-Min Choi, Jeong-Ho Lee, Byung-Taek Oh Pseudomonas and Bacillus species are attractive due to their potential bio-control application against plant bacterial pathogens. Pseudomonas aeruginosa strain D4 and Bacillus stratosphericus strain FW3 were isolated from mine tailings in South Korea. In these potent bacterial strains, we observed improved antagonistic activity against Pseudomonas syringae DC3000. These st...
Source: Microbiological Research - September 13, 2017 Category: Infectious Diseases Source Type: research

Assessment of active bacteria metabolizing phenolic acids in the peanut (Arachis hypogaea L.) rhizosphere
The objectives of this study were to isolate and characterize bacteria capable of growth on benzoic and vanillic acids as the sole carbon source in the peanut rhizosphere. Twenty-four bacteria were isolated, and the identification based on 16S rRNA gene sequencing revealed that pre-exposure to phenolic acids before sowing shifted the dominant culturable bacterial degraders from Arthrobacter to Burkholderia stabilis-like isolates. Both Arthrobacter and B. stabilis-like isolates catalysed the aromatic ring cleavage via the ortho pathway, and Arthrobacter isolates did not exhibit higher C12O enzyme activity than B. stabilis-l...
Source: Microbiological Research - September 10, 2017 Category: Infectious Diseases Source Type: research

Nucleoside diphosphate kinase (Ndk): A pleiotropic effector manipulating bacterial virulence and adaptive responses
Publication date: Available online 8 September 2017 Source:Microbiological Research Author(s): Hua Yu, Xiancai Rao, Kebin Zhang Nucleoside diphosphate kinase (Ndk) is a housekeeping enzyme that balances cellular nucleoside triphosphate (NTP) pools by catalyzing the reversible transfer of γ-phosphate from NTPs to nucleoside diphosphates (NDPs). In addition to its fundamental role in nucleotide metabolism, Ndk has roles in protein histidine phosphorylation, DNA cleavage/repair, and gene regulation. Recent studies have also revealed that Ndk secreted from bacteria is important in modulating virulence-associated phenoty...
Source: Microbiological Research - September 9, 2017 Category: Infectious Diseases Source Type: research