From Laboratory to Clinic: Translation of Extracellular Vesicle Based Cancer Biomarkers
Publication date: Available online 12 February 2020Source: MethodsAuthor(s): Anudeep Yekula, Koushik Muralidharan, Keiko Kang, Lan Wang, Leonora Balaj, Bob S. CarterAbstractThe past decade has witnessed a rapid growth in the field of extracellular vesicle (EV) based biomarkers for the diagnosis and monitoring of cancer. Several studies have reported novel EV based biomarkers, but the technical and clinical validation phase has been hampered by general challenges common to biomedical research field as well as specific challenges inherent to the nanoparticle field. This has led to more common failures than success stories in...
Source: Methods - February 13, 2020 Category: Molecular Biology Source Type: research
Lactobionamide-based fluorinated detergent for functional and structural stabilization of membrane proteins
We report herein the use of a fluorinated lactobionamide-based detergent named FLAC6 for functional and structural stabilization of membrane proteins. We first demonstrated that FLAC6 efficiently solubilized three membrane proteins i.e. the native adenosine receptor A2AR, a G protein-coupled receptor, and two native transporters AcrB and BmrA. The resulting affinity purified MPs were highly pure, homogenous and aggregates free. Furthermore, the functionality of each MP was well maintained. Finally, striking overstabilization features were observed. Indeed, the Tm of native A2AR, AcrB and BmrA could be improved by 7, ∼9 a...
Source: Methods - February 13, 2020 Category: Molecular Biology Source Type: research
SpineJ: A software tool for quantitative analysis of nanoscale spine morphology
We present SpineJ, a semi-automatic ImageJ plugin that is specifically designed for this purpose. SpineJ offers an intuitive and user-friendly graphical user interface, facilitating fast, accurate, and unbiased analysis of spine morphology. (Source: Methods)
Source: Methods - February 8, 2020 Category: Molecular Biology Source Type: research
Assessment of separation methods for extracellular vesicles from human and mouse brain tissues and human cerebrospinal fluids
Publication date: Available online 5 February 2020Source: MethodsAuthor(s): Satoshi Muraoka, Weiwei Lin, Mei Chen, Samuel W. Hersh, Andrew Emili, Weiming Xia, Tsuneya IkezuAbstractExtracellular vesicle (EV) is a unified terminology of membrane-enclosed vesicular species ubiquitously secreted by almost every cell type and present in all body fluids. They carry a cargo of lipids, metabolites, nucleic acids and proteins for their clearance from cells as well as for cell-to-cell communications. The exact composition of EVs and their specific functions are not well understood due to the underdevelopment of the separation protoc...
Source: Methods - February 6, 2020 Category: Molecular Biology Source Type: research
Using inverse finite element analysis to identify spinal tissue behaviour in situ
Publication date: Available online 6 February 2020Source: MethodsAuthor(s): Mengoni MarlèneAbstractIn computational modelling of musculoskeletal applications, one of the critical aspects is ensuring that a model can capture intrinsic population variability and not only representative of a “mean” individual. Developing and calibrating models with this aspect in mind is key for the credibility of a modelling methodology. This often requires calibration of complex models with respect to 3D experiments and measurements on a range of specimens or patients. Most Finite Element (FE) software’s do not have such a capacity e...
Source: Methods - February 6, 2020 Category: Molecular Biology Source Type: research
