Establishing a framework for best practices for quality assurance and quality control in untargeted metabolomics
AbstractBackgroundQuality assurance (QA) and quality control (QC) practices are key tenets that facilitate study and data quality across all applications of untargeted metabolomics. These important practices will strengthen this field and accelerate its success. The Best Practices Working Group (WG) within the Metabolomics Quality Assurance and Quality Control Consortium (mQACC) focuses on community use of QA/QC practices and protocols and aims to identify, catalogue, harmonize, and disseminate current best practices in untargeted metabolomics through community-driven activities.Aim of reviewA present goal of the Best Prac...
Source: Metabolomics - February 12, 2024 Category: Biology Source Type: research
Circadian rhythm in turbot (Scophthalmus maximus): daily variation of blood metabolites in recirculating aquaculture systems
ConclusionThis study provides initial insights into the diurnal variation of metabolites in adult turbot; however, further studies are needed to confirm present findings of possible fluctuations in amino acids and sugars. Implementing optimized feeding times (with high levels of sugars and low levels of stress metabolites) could lead to less stress, fewer disease outbreaks and overall improved fish welfare in aquaculture facilities. (Source: Metabolomics)
Source: Metabolomics - February 12, 2024 Category: Biology Source Type: research
Evaluation of normalization strategies for GC-based metabolomics
ConclusionTUPA and TDPA carry different strengths: TUPA requires peak alignment across all samples, which depends upon the completion of the study, while TDPA is free from the requirement of alignment. The findings of the study would enhance the convenient and effective use of data normalization strategies and contribute to overcoming the data normalization challenges that currently exist in the metabolomics community. (Source: Metabolomics)
Source: Metabolomics - February 12, 2024 Category: Biology Source Type: research
Exploration of differential responses to FODMAPs and gluten in people with irritable bowel syndrome- a double-blind randomized cross-over challenge study
ConclusionDifferential IBS responses to FODMAPs or gluten exposures could not be explained from clinical and molecular data despite extensive exploration with different data analytical approaches. The trial is registered atwww.clinicaltrials.gov as NCT03653689 31/08/2018. (Source: Metabolomics)
Source: Metabolomics - February 12, 2024 Category: Biology Source Type: research
Circadian rhythm in turbot (Scophthalmus maximus): daily variation of blood metabolites in recirculating aquaculture systems
ConclusionThis study provides initial insights into the diurnal variation of metabolites in adult turbot; however, further studies are needed to confirm present findings of possible fluctuations in amino acids and sugars. Implementing optimized feeding times (with high levels of sugars and low levels of stress metabolites) could lead to less stress, fewer disease outbreaks and overall improved fish welfare in aquaculture facilities. (Source: Metabolomics)
Source: Metabolomics - February 12, 2024 Category: Biology Source Type: research
Evaluation of normalization strategies for GC-based metabolomics
ConclusionTUPA and TDPA carry different strengths: TUPA requires peak alignment across all samples, which depends upon the completion of the study, while TDPA is free from the requirement of alignment. The findings of the study would enhance the convenient and effective use of data normalization strategies and contribute to overcoming the data normalization challenges that currently exist in the metabolomics community. (Source: Metabolomics)
Source: Metabolomics - February 12, 2024 Category: Biology Source Type: research
Establishing a framework for best practices for quality assurance and quality control in untargeted metabolomics
AbstractBackgroundQuality assurance (QA) and quality control (QC) practices are key tenets that facilitate study and data quality across all applications of untargeted metabolomics. These important practices will strengthen this field and accelerate its success. The Best Practices Working Group (WG) within the Metabolomics Quality Assurance and Quality Control Consortium (mQACC) focuses on community use of QA/QC practices and protocols and aims to identify, catalogue, harmonize, and disseminate current best practices in untargeted metabolomics through community-driven activities.Aim of reviewA present goal of the Best Prac...
Source: Metabolomics - February 12, 2024 Category: Biology Source Type: research
Induced responses to the wheat pathogen: Tan Spot —(Pyrenophora tritici-repentis) in wheat (Triticum aestivum) focus on changes in defence associated and sugar metabolism
ConclusionThese observations suggest the potential importance of flavone and flavonol biosynthesis as well as bioenergetic shifts in susceptibility toPtr. This work highlights the value of metabolomic approaches to provide novel insights into wheat pathosystems. (Source: Metabolomics)
Source: Metabolomics - January 31, 2024 Category: Biology Source Type: research
Urine and serum metabolomic analysis of endometrial cancer diagnosis and classification based on ultra-performance liquid chromatography mass spectrometry
ConclusionThe combined urine and serum metabolome effectively revealed the metabolic patterns in EC patients, offering valuable diagnostic models for EC diagnosis and classification. (Source: Metabolomics)
Source: Metabolomics - January 28, 2024 Category: Biology Source Type: research
Classifying patients with psoriatic arthritis according to their disease activity status using serum metabolites and machine learning
ConclusionSeveral lipids and other metabolites when combined in classifying models predict high disease activity from both low and moderate disease activity. Lipids of key interest included lysophosphatidylcholine and sphingomyelin. Quantitative MS assays based on selected reaction monitoring, are required to quantify the candidate biomarkers identified. (Source: Metabolomics)
Source: Metabolomics - January 24, 2024 Category: Biology Source Type: research
Metabolomic profiling of human bladder tissue extracts
ConclusionTo date, this is the first study in which bladder human normal tissues adjacent to cancerous tissues are analyzed using both NMR and MS method. These findings suggest that the metabolite markers identified in this study may be useful for the detection and monitoring of bladder cancer stages and grades. (Source: Metabolomics)
Source: Metabolomics - January 24, 2024 Category: Biology Source Type: research
Metabolomic data presents challenges for epidemiological meta-analysis: a case study of childhood body mass index from the ECHO consortium
ConclusionsDespite including three cohorts with large sample sizes (N > 100), we failed to identify significant metabolite associations after FDR correction. Our investigation demonstrates difficulties in applying epidemiological meta-analysis to clinical metabolomics, emphasizes challenges to reproducibility, and highlights the need for standardized best practices i n metabolomic epidemiology. (Source: Metabolomics)
Source: Metabolomics - January 24, 2024 Category: Biology Source Type: research
Challenges and perspectives for naming lipids in the context of lipidomics
ConclusionHomogenising this identification and adopting the same rules is essential to be able to share data within the community and to map data on functional networks. (Source: Metabolomics)
Source: Metabolomics - January 24, 2024 Category: Biology Source Type: research
