A metabolic readout of the urine metabolome of COVID-19 patients
AbstractAnalysis of urine samples from COVID-19 patients by1H NMR reveals important metabolic alterations due to SAR-CoV-2 infection. Previous studies have identified biomarkers in urine that reflect metabolic alterations in COVID-19 patients. We have used1H NMR to better define these metabolic alterations since this technique allows us to obtain a broad profile of the metabolites present in urine. This technique offers the advantage that sample preparation is very simple and gives us very complete information on the metabolites present. To detect these alterations, we have compared urine samples from COVID-19 patients (n ...
Source: Metabolomics - January 24, 2023 Category: Biology Source Type: research

Multi-omics profiling of PC-3 cells reveals bufadienolides-induced lipid metabolic remodeling by regulating long-chain lipids synthesis and hydrolysis
ConclusionsAll these findings support that bufadienolides might induce lipid metabolic remodeling by regulating long-chain lipids synthesis and phospholipid hydrolysis to achieve an anti-prostate cancer effect, and PLD1 would probably be the key protein. (Source: Metabolomics)
Source: Metabolomics - January 16, 2023 Category: Biology Source Type: research

Acute metabolic alterations in the hippocampus are associated with decreased acetylation after blast induced TBI
ConclusionThe present study reports altered acetylation in the presence of altered metabolism in hippocampal tissue of blast induced rmTBI at 24  h post injury. Mechanistic understanding of these intertwined processes may help in the development of better therapeutic pathways and agents for blast induced TBI in near future. (Source: Metabolomics)
Source: Metabolomics - January 12, 2023 Category: Biology Source Type: research

Guidelines and considerations for building multidimensional libraries for untargeted MS-based metabolomics
DiscussionThe outcome was an open-access available NIST format metabolite library (.msp) with multidimensional information. The library was used to evaluate CCS prediction tools, MS/MS spectra heterogeneities (e.g., multiple adducts, in-source fragmentation, radical fragment ions using collision-induced dissociation), and the reporting of RT. (Source: Metabolomics)
Source: Metabolomics - December 28, 2022 Category: Biology Source Type: research

Untargeted metabolomics and lipidomics identified four subtypes of small cell lung cancer
ConclusionOur study revealed the metabolic heterogeneity in SCLC and identified four subtypes with distinct metabolic features. It indicates promising therapeutic and prognostic value that may guide treatment for SCLC. The subtype-specific clinical trials may be designed and would be instructive for drug development. (Source: Metabolomics)
Source: Metabolomics - December 27, 2022 Category: Biology Source Type: research

Methyl jasmonate induces selaginellin accumulation in Selaginella convoluta
In this study, we examined MeJA elicitation inSelaginella convoluta plants, a medicinal plant found in northeastern Brazil, by treating them with two different concentrations (MeJA: 50 and 100  µM), followed by chemical profiling after 12, 24 and 48 h after application. Samples were harvested and analyzed by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS).Results and discusscionMeJA treatment significantly impacted the chemical phenotype. Regarding shoots differences in the time-dependent increased accumulation of all metabolites when plants were subjected to 100  µM MeJA were observed while in ro...
Source: Metabolomics - December 21, 2022 Category: Biology Source Type: research

Maternal exposure to polystyrene microplastics alters placental metabolism in mice
ConclusionMaternal exposure to microplastics resulted in significant alterations in placental metabolism. This study highlights the potential impact of microplastic exposure on pregnancy outcomes and that efforts should be made to minimize exposure to plastics, particularly during pregnancy. (Source: Metabolomics)
Source: Metabolomics - December 20, 2022 Category: Biology Source Type: research

Providing metabolomics education and training: pedagogy and considerations
AbstractBackgroundMetabolomics is a highly multidisciplinary and non-standardised research field. Metabolomics researchers must possess and apply extensive cross-disciplinary content knowledge, subjective experience-based judgement, and the associated diverse skill sets. Accordingly, appropriate educational and training initiatives are important in developing this knowledge and skills base in the metabolomics community. For these initiatives to be successful, they must consider both pedagogical best practice and metabolomics-specific contextual challenges.Aim of reviewThe aim of this review is to provide consolidated pedag...
Source: Metabolomics - December 13, 2022 Category: Biology Source Type: research