Quantitative metabolome profiling reveals the involvement of the kynurenine pathway in influenza-associated encephalopathy
Conclusion Comprehensive metabolite profiles revealed five metabolites as potential biomarkers for influenza-associated encephalopathy; the tryptophan–kynurenine metabolic process could be associated with its pathophysiology. (Source: Metabolomics)
Source: Metabolomics - March 15, 2016 Category: Biology Source Type: research

Real-time metabolomic analysis of lactic acid bacteria as monitored by in vitro NMR and chemometrics
Conclusion The developed analytical protocol for real-time in vitro NMR measurements of bacterial metabolism allows a relatively easy investigation of different fermentation factors such as new strains, new substrates, cohabitations, temperature, and pH and has a great potential in biopreservation studies to discover new efficient bioprotective cultures. (Source: Metabolomics)
Source: Metabolomics - March 14, 2016 Category: Biology Source Type: research

Metabolomics reveals effects of maternal smoking on endogenous metabolites from lipid metabolism in cord blood of newborns
Conclusion Our data give evidence that smoking during pregnancy alters both the maternal and children’s metabolome. Whether the different pattern found in adults compared to newborn children could be related to different disease outcomes should be in the focus of future studies. (Source: Metabolomics)
Source: Metabolomics - March 8, 2016 Category: Biology Source Type: research

Metabolomic signatures of increases in temperature and ocean acidification from the reef-building coral, Pocillopora damicornis
Conclusions The integration of metabolite and physiological data highlights the predictive power of metabolomics in defining organism performance and provides biomarkers for future studies. Here, we present a multivariate biomarker approach to assess climate change impacts and advance our mechanistic understanding of stress response in this keystone species. (Source: Metabolomics)
Source: Metabolomics - March 8, 2016 Category: Biology Source Type: research

Alkaloid biosynthesis and metabolic profiling responses to jasmonic acid elicitation in Hamelia patens plants by NMR-based metabolomics
Conclusion Correlations between primary and secondary metabolism biosynthetic pathways, such as the alkaloid and chlorogenic acid biosynthesis were found. Also, their implications in the plant fitness to stress are proposed. Confirmation of tryptophan as a precursor of MIA and MOA, via shikimate pathway, was done by L-[3′-14C]-tryptophan feeding. (Source: Metabolomics)
Source: Metabolomics - March 8, 2016 Category: Biology Source Type: research

Metabolomics of biomarker discovery in ovarian cancer: a systematic review of the current literature
Conclusion Metabolomics presents significant new opportunities for diagnostic biomarker development, elucidating previously unknown mechanisms of OC pathogenesis. (Source: Metabolomics)
Source: Metabolomics - March 8, 2016 Category: Biology Source Type: research

Understanding the Jasmine phenotype of rice through metabolite profiling and sensory evaluation
Conclusions The results of this study are valuable in understanding the aspects of aromatic rice that are important to consumers, and in the identification of compounds that breeding programs can use to select for pleasant aromas, enabling breeding programs to target markets with greater accuracy. (Source: Metabolomics)
Source: Metabolomics - March 8, 2016 Category: Biology Source Type: research

New molecular evidence of wine yeast-bacteria interaction unraveled by non-targeted exometabolomic profiling
Conclusion The non-targeted approach used in this study played an important role in finding new and unexpected molecular evidence. (Source: Metabolomics)
Source: Metabolomics - March 8, 2016 Category: Biology Source Type: research

Identifying metabolite markers for preterm birth in cervicovaginal fluid by magnetic resonance spectroscopy
Abstract Introduction Preterm birth (PTB) may be preceded by changes in the vaginal microflora and metabolite profiles. Objectives We sought to characterise the metabolite profile of cervicovaginal fluid (CVF) of pregnant women by 1H NMR spectroscopy, and assess their predictive value for PTB. Methods A pair of high-vaginal swabs was obtained from pregnant women with no evidence of clinical infection and grouped as follows: asymptomatic low risk (ALR) women with no previous history of PTB, assessed ...
Source: Metabolomics - March 8, 2016 Category: Biology Source Type: research

High resolution metabolomic analysis of ASD human brain uncovers novel biomarkers of disease
This study aimed to biochemically profile the cerebellum from post-mortem human brain from ASD sufferers (n = 11) and compare their profiles to that of age-matched controls (n = 11) with no known brain disorder. Methods Using liquid chromatography combined with LTQ-Orbitrap mass spectrometry we detected 14,328 features in ESI+ mode in polar extracts of post-mortem brain. Results Of these only 37 were found to be statistically significantly different between ASD and controls (p 
Source: Metabolomics - March 8, 2016 Category: Biology Source Type: research

Guinea pig genital tract lipidome reveals in vivo and in vitro regulation of phosphatidylcholine 16:0/18:1 and contribution to Chlamydia trachomatis serovar D infectivity
Conclusion This study demonstrates a role for host metabolite, PC 16:0/18:1 in regulating genital Ct infection in vivo and in vitro. (Source: Metabolomics)
Source: Metabolomics - March 8, 2016 Category: Biology Source Type: research

Monitoring spatial and temporal metabolic dynamics of woody poplar root under mechanical stress conditions by NMR-based metabolomics
Conclusion The data demonstrate that the impact of mechanical stress on plant biology can efficiently be studied by NMR-based metabolomics. (Source: Metabolomics)
Source: Metabolomics - March 8, 2016 Category: Biology Source Type: research

An efficient method for medium throughput screening of cuticular wax composition in different plant species
Conclusion This protocol allows standardized identification and quantification of most cuticular wax components in a range of species. (Source: Metabolomics)
Source: Metabolomics - March 8, 2016 Category: Biology Source Type: research

Differential metabolomic responses of PAMP-triggered immunity and effector-triggered immunity in Arabidopsis suspension cells
Conclusion PstDC3000D28E triggered PTI responses were different from those of flg22. This study has not only revealed the discernible metabolomics features associated with the flg22, PstDC3000D28E and PstDC3000 treatments, but also laid a foundation toward further understanding of metabolic regulation and responses underlying plant PTI and ETI. (Source: Metabolomics)
Source: Metabolomics - March 8, 2016 Category: Biology Source Type: research

Characterisation of the main drivers of intra- and inter- breed variability in the plasma metabolome of dogs
Conclusion Metabolite fingerprinting of plasma samples can be used to investigate breed differences in client-owned dogs, despite added variance of diet, sexual status and environment. (Source: Metabolomics)
Source: Metabolomics - March 8, 2016 Category: Biology Source Type: research

A novel metabolomic approach used for the comparison of Staphylococcus aureus planktonic cells and biofilm samples
Conclusions The method described herein represents a valuable tool in studying microbial biochemistry at a molecular level. While the methodology is generally applicable to the lysis and extraction of metabolites from Gram positive bacteria, it is particularly applicable to biofilms. Bacteria that exist as a biofilm are shown to be highly distinct metabolically from their ‘free living’ counterparts, thus highlighting the need to study microbes in different growth states. Metabolomics can successfully distinguish between a planktonic and biofilm growth state. Importantly, this study design, incorporat...
Source: Metabolomics - March 8, 2016 Category: Biology Source Type: research

Calibration curve-free GC–MS method for quantitation of amino and non-amino organic acids in biological samples
Conclusion Our novel approach gives the potential to identify and quantify hundreds of metabolites, far exceeding the capabilities of any absolute quantitative targeted metabolite analysis, limited only by the size of the mass spectral library. (Source: Metabolomics)
Source: Metabolomics - March 8, 2016 Category: Biology Source Type: research

LC–MS/MS-based metabolome analysis detected changes in the metabolic profiles of small and large intestinal adenomatous polyps in Apc Min/+ mice
Conclusion Our results indicate that various metabolic changes are associated with polyp development, and understanding these alterations could make it possible to evaluate the treatment response of colorectal cancer earlier. (Source: Metabolomics)
Source: Metabolomics - March 8, 2016 Category: Biology Source Type: research

Characterization of ankylosing spondylitis and rheumatoid arthritis using 1 H NMR-based metabolomics of human fecal extracts
Conclusion The metabolome variations in feces indicated AS and RA were two homologous diseases that could not be distinguished by 1H NMR metabolomics. (Source: Metabolomics)
Source: Metabolomics - March 8, 2016 Category: Biology Source Type: research

A targeted metabolomics assay for cardiac metabolism and demonstration using a mouse model of dilated cardiomyopathy
We describe a targeted metabolomics assay that quantifies a broad range of over 130 metabolites relevant to cardiac metabolism including the pathways of the citric acid cycle, fatty acid oxidation, glycolysis, the pentose phosphate pathway, amino acid metabolism, the urea cycle, nucleotides and reactive oxygen species using tandem mass spectrometry to produce quantitative, sensitive and robust data. This assay is illustrated by profiling cardiac metabolism in a lamin A/C (Lmna) mouse model of dilated cardiomyopathy (DCM). The model of DCM was characterised by increases in concentrations of proline and methyl-histidine sugg...
Source: Metabolomics - March 7, 2016 Category: Biology Source Type: research

Temporal characterization of serum metabolite signatures in lung cancer patients undergoing treatment
Abstract Lung cancer causes more deaths in men and women than any other cancer related disease. Currently, few effective strategies exist to predict how patients will respond to treatment. We evaluated the serum metabolomic profiles of 25 lung cancer patients undergoing chemotherapy ± radiation to evaluate the feasibility of metabolites as temporal biomarkers of clinical outcomes. Serial serum specimens collected prospectively from lung cancer patients were analyzed using both nuclear magnetic resonance (1H-NMR) spectroscopy and gas chromatography mass spectrometry (GC–MS). Multivariate sta...
Source: Metabolomics - February 27, 2016 Category: Biology Source Type: research

Metabolite profiling of the ripening of Mangoes Mangifera indica L. cv. ‘Tommy Atkins’ by real-time measurement of volatile organic compounds
Abstract Real-time profiling of mango ripening based on proton transfer reaction-time of flight-mass spectrometry (PTR–ToF–MS) of small molecular weight volatile organic compounds (VOCs), is demonstrated using headspace measurements of ‘Tommy Atkins’ mangoes. VOC metabolites produced during the ripening process were sampled directly, which enabled simultaneous and rapid detection of a wide range of compounds. Headspace measurements of ‘Keitt’ mangoes were also conducted for comparison. A principle component analysis of the results indicated that several mass channels were not o...
Source: Metabolomics - February 18, 2016 Category: Biology Source Type: research

A batch correction method for liquid chromatography–mass spectrometry data that does not depend on quality control samples
Abstract The need for reproducible and comparable results is of increasing importance in non-targeted metabolomic studies, especially when differences between experimental groups are small. Liquid chromatography–mass spectrometry spectra are often acquired batch-wise so that necessary calibrations and cleaning of the instrument can take place. However this may introduce further sources of variation, such as differences in the conditions under which the acquisition of individual batches is performed. Quality control (QC) samples are frequently employed as a means of both judging and correcting this variation...
Source: Metabolomics - February 18, 2016 Category: Biology Source Type: research

Erratum to: Tuberculosis metabolomics reveals adaptations of man and microbe in order to outcompete and survive
(Source: Metabolomics)
Source: Metabolomics - February 17, 2016 Category: Biology Source Type: research

Say hello to Dr Warwick Dunn! Metabolomics’ new Reviews Editor
(Source: Metabolomics)
Source: Metabolomics - February 16, 2016 Category: Biology Source Type: research

Untangling the wine metabolome by combining untargeted SPME–GCxGC-TOF-MS and sensory analysis to profile Sauvignon blanc co-fermented with seven different yeasts
This study aimed to compare untargeted volatile compound profiles from SPME–GCxGC-TOF-MS and sensory analysis data of Sauvignon blanc wine inoculated with six different non-Saccharomyces yeasts followed by SC. Torulaspora delbrueckii (TD), Lachancea thermotolerans (LT), Pichia kluyveri (PK) and Metschnikowia pulcherrima (MP) where commercial starter strains, while Candida zemplinina (CZ) and Kazachstania aerobia (KA), were isolated from wine grape environments. Each wine showed a distinct profile both sensorially and chemically. SC and CZ wines were the most distinct in both of these cases. SC wine had guava, grapefr...
Source: Metabolomics - February 13, 2016 Category: Biology Source Type: research

Metabolite targeting: development of a comprehensive targeted metabolomics platform for the assessment of diabetes and its complications
Abstract Biomarker studies for metabolic disorders like diabetes mellitus (DM) are an important approach towards a better understanding of the underlying pathophysiological mechanisms of diseases (Roberts and Gerszten in Cell Metab 18:43–50, 2013; Wilson et al. in Proteome Res 4:591–598, 2005). Furthermore, screening of potential metabolic biomarkers opens the opportunity of early diagnosis as well as therapy and drug monitoring of metabolic disorders (Rhee et al. in J Clin Invest 10:1–10, 2011; Wang et al. in Nat Med 17:448–458, 2011; Wenk in Nat Rev Drug Discov 4:594–610, 2005). Th...
Source: Metabolomics - February 9, 2016 Category: Biology Source Type: research

Changes in serum fatty acid and lipoprotein subclass concentrations from prepuberty to adulthood and during aging
Abstract Concentrations in serum were determined for 18 fatty acids (FAs) and 21 lipoprotein main and subclasses by chromatographic analyses and the average size was calculated for very low density (VLDL), low density (LDL) and high density (HDL) particles. 283 ethnic Norwegian children and adults from the rural Fjord region of Western Norway were compared with the objectives to reveal patterns and gender differences during the development from prepuberty to adulthood and during aging in adults. Both genders showed a large increase in eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) from child to adult....
Source: Metabolomics - February 8, 2016 Category: Biology Source Type: research

Metabolomics as a tool for the authentication of rose extracts used in flavour and fragrance area
Abstract Natural extracts used in flavour and fragrances are exposed to authentication issues. Companies working in this industrial market acquire the raw materials locally but also abroad, sourcing exotic plants with specific olfactive features or lower costs of production. The geographical origin, the botanical variety, environmental conditions, extraction processes and storage conditions represent some parameters affecting the natural extract quality. All these factors are likely to affect the sensorial properties and especially the organoleptic characteristics of the extract. In addition, fraudulent practices...
Source: Metabolomics - February 8, 2016 Category: Biology Source Type: research

Metabolomic study of the bone trabecula of osteonecrosis femoral head patients based on UPLC–MS/MS
Abstract The severity and/or progression of osteonecrosis of the femoral head (ONFH) are commonly assessed by radiography, nuclear magnetic resonance image which aren’t invariably correlated to severity of disease and may be disturbed by other factors. Consequently, exploring the novel biochemical signatures of ONFH may be beneficial for diagnosing and understanding this disease. In this work, a bone trabecula metabolomics was undertaken to determine the expression pattern of low molecular mass metabolites in patients of femoral head necrosis based on the ultra-performance liquid chromatography/time-of-flig...
Source: Metabolomics - February 8, 2016 Category: Biology Source Type: research

Ion mobility mass spectrometry enhances low-abundance species detection in untargeted lipidomics
We describe a simple method for the detection of low intensity lipid signals in complex tissue samples, based on a combination of liquid chromatography/mass spectrometry and ion mobility mass spectrometry. The method relies on visual and software-assisted analysis of overlapped mobilograms (diagrams of mass-to-charge ratio, m/z, vs drift time, DT) and was successfully applied in untargeted lipidomics analyses of mouse brain tissue to detect relatively small variations in a scarce class of phospholipids (N-acyl phosphatidylethanolamines) generated during neural tissue damage, against a background of hundreds of lipid specie...
Source: Metabolomics - February 8, 2016 Category: Biology Source Type: research

Metabolic effects of cannabinoids in zebrafish ( Danio rerio ) embryos determined by 1 H NMR metabolomics
Abstract The study of drug-driven biochemical changes is important in order to determine the biomarkers associated with a specific compound activity in an individual biological system. Rodent models have been widely used to study the metabolic changes induced by psychostimulants in a cell, tissue or whole organism. However these models are not suitable for large-scale, high-throughput screening. Here, we used zebrafish embryos to study the metabolic effects of the cannabinoid receptor type 1 (CB1) agonist (−)-(6aR,10aR)-6,6,9-trimethyl-3-pentyl-6a,7,8,10a-tetrahydro-6H-benzo[c]chromen-1-ol (∆9-THC) and an...
Source: Metabolomics - February 5, 2016 Category: Biology Source Type: research

Metabolic and transcriptomic profiling of Streptococcus intermedius during aerobic and anaerobic growth
This study provides a better understanding of differences between S. intermedius anaerobic and aerobic metabolism. The results reflect the organism’s predilection for anaerobic growth consistent with its pathogenic association with anaerobes in polymicrobial infections. (Source: Metabolomics)
Source: Metabolomics - February 5, 2016 Category: Biology Source Type: research

Obesity-related metabolite profiles of black women spanning the epidemiologic transition
Abstract In developed countries, specific metabolites have been associated with obesity and metabolic diseases, e.g. type 2 diabetes. It is unknown whether a similar profile persists across populations of African-origin, at increased risk for obesity and related diseases. In a cross-sectional study of normal-weight and obese black women (33.3 ± 6.3 years) from the US (N = 69, 65 % obese), South Africa (SA, N = 97, 49 % obese) and Ghana (N = 82, 33 % obese) serum metabolite profiles were characterized via gas chromatography-time of flight/mass ...
Source: Metabolomics - February 5, 2016 Category: Biology Source Type: research

Effects of heat-treatment on the stability and composition of metabolomic extracts from the earthworm Eisenia fetida
This study evaluated the efficacy of adding a heat-treatment step in two commonly used extraction protocols (Bligh and Dyer and D2O phosphate buffer) as a pre-analytical stabilization method. The resulting metabolic profiles of Eisenia fetida were assessed using principal component analysis and NMR spectral evaluations. The heated Bligh and Dyer extractions produced stabilized profiles with minimal variation of the extracted metabolomic profiles over time, providing a more suitable method for metabolomic analysis of earthworm extracts. (Source: Metabolomics)
Source: Metabolomics - February 5, 2016 Category: Biology Source Type: research

MSCombine: a tool for merging untargeted metabolomic data from high-resolution mass spectrometry in the positive and negative ionization modes
Abstract The wide chemical diversity of metabolites present in biological samples hinders full characterization of their metabolomic profile by using a single analytical technique. However, LC–MS is a competitive technique for obtaining a comprehensive description of a wide range of metabolites by using a combination of the two ionization modes, positive and negative, the suitability of which for this purpose depends on the chemical structure of the particular metabolites. One of the main shortcomings of this approach is that it produces two independent data sets (one per polarity) that require two para...
Source: Metabolomics - February 4, 2016 Category: Biology Source Type: research

Tuberculosis metabolomics reveals adaptations of man and microbe in order to outcompete and survive
Abstract Despite numerous research efforts to control tuberculosis, it is still regarded as a global pandemic. It is clear that the infectious agent responsible and its associated disease mechanisms remain poorly understood. Alternative research strategies are therefore urgently needed to better characterize active-TB, especially the adaptations of the host and microbe as they compete to survive. Using a GCxGC-TOFMS metabolomics approach, we identified new urinary TB metabolite markers induced by adaptations of the host metabolome and/or host-pathogen interactions. The most significant of these were the TB-induce...
Source: Metabolomics - February 3, 2016 Category: Biology Source Type: research

Identification of candidate biomarkers of brain damage in a mouse model of closed head injury: a metabolomic pilot study
Abstract We aim to identify candidate brain biomarkers for, and to elucidate the pathophysiology of closed traumatic brain injury (TBI). Nuclear magnetic resonance (NMR) based metabolomic analysis was performed on the whole brain of mice undergoing TBI using a validated technique. There were 10 TBI mice compared to 8 sham operated controls. A total of 45 metabolites were evaluated. There was a statistically significant alteration in concentrations of 29 metabolites in TBI brains as compared to controls (FDR
Source: Metabolomics - February 3, 2016 Category: Biology Source Type: research

Citrus tristeza virus infection in sweet orange trees and a mandarin × tangor cross alters low molecular weight metabolites assessed using gas chromatography mass spectrometry (GC/MS)
In this study, we analyzed leaf metabolite extracts from six sweet orange varieties and a mandarin × tangor cross infected with CTV collected at the Lindcove Research and Extension Center (LREC; Exeter, CA). In order to analyze low volatility small molecules, the extracts of leaf metabolites were derivatized by N-methyl-N-trimethylsilyl-trifluoracetamide (MSTFA). Chemical analysis was performed with gas chromatography/mass spectrometry (GC/MS) to assess metabolite changes induced by CTV infection. Principal Component Analysis (PCA) and Hotelling’s T2 were used to identify outliers within the set of samples. Par...
Source: Metabolomics - February 3, 2016 Category: Biology Source Type: research

Multi-platform metabolomics analyses of a broad collection of fragrant and non-fragrant rice varieties reveals the high complexity of grain quality characteristics
Abstract The quality of rice in terms not only of its nutritional value but also in terms of its aroma and flavour is becoming increasingly important in modern rice breeding where global targets are focused on both yield stability and grain quality. In the present paper we have exploited advanced, multi-platform metabolomics approaches to determine the biochemical differences in 31 rice varieties from a diverse range of genetic backgrounds and origin. All were grown under the specific local conditions for which they have been bred and all aspects of varietal identification and sample purity have been guaranteed b...
Source: Metabolomics - January 23, 2016 Category: Biology Source Type: research

GC–MS metabolic profiling of Cabernet Sauvignon and Merlot cultivars during grapevine berry development and network analysis reveals a stage- and cultivar-dependent connectivity of primary metabolites
Abstract Information about the total chemical composition of primary metabolites during grape berry development is scarce, as are comparative studies trying to understand to what extent metabolite modifications differ between cultivars during ripening. Thus, correlating the metabolic profiles with the changes occurring in berry development and ripening processes is essential to progress in their comprehension as well in the development of new approaches to improve fruit attributes. Here, the developmental metabolic profiling analysis across six stages from flowering to fully mature berries of two cultivars, Caber...
Source: Metabolomics - January 23, 2016 Category: Biology Source Type: research

Erratum to: Informative metabolites identification by variable importance analysis based on random variable combination
(Source: Metabolomics)
Source: Metabolomics - January 22, 2016 Category: Biology Source Type: research

Metabolomics connects aberrant bioenergetic, transmethylation, and gut microbiota in sarcoidosis
Abstract Sarcoidosis is a systemic granulomatous disease of unknown etiology. Granulomatous inflammation in sarcoidosis may affect multiple organs, including the lungs, skin, CNS, and the eyes, leading to severe morbidity and mortality. The underlying mechanisms for sustained inflammation in sarcoidosis are unknown. We hypothesized that metabolic changes play a critical role in perpetuation of inflammation in sarcoidosis. 1H nuclear magnetic resonance (NMR)-based untargeted metabolomic analysis was used to identify circulating molecules in serum to discriminate sarcoidosis patients from healthy controls. Principa...
Source: Metabolomics - January 20, 2016 Category: Biology Source Type: research

Incorporation of omics analyses into artificial gravity research for space exploration countermeasure development
Abstract The next major steps in human spaceflight include flyby, orbital, and landing missions to the Moon, Mars, and near earth asteroids. The first crewed deep space mission is expected to launch in 2022, which affords less than 7 years to address the complex question of whether and how to apply artificial gravity to counter the effects of prolonged weightlessness. Various phenotypic changes are demonstrated during artificial gravity experiments. However, the molecular dynamics (genotype and molecular phenotypes) that underlie these morphological, physiological, and behavioral phenotypes are far more comp...
Source: Metabolomics - January 20, 2016 Category: Biology Source Type: research

Increasing lipidomic coverage by selecting optimal mobile-phase modifiers in LC–MS of blood plasma
Abstract Reversed-phase ultrahigh-performance liquid chromatography–mass spectrometry (UHPLC–MS) is the method of choice for lipidomic profiling of blood plasma. For comprehensive screening, lipids need to be screened in both positive and negative electrospray mode. We here show that optimal results for increased lipidome coverage are only obtained if two different mobile-phase modifier systems are used, as opposed to using a single common modifier for both ionization modes. Specifically, ammonium acetate and ammonium formate were tested with and without acidifiers on standards representing 16 lipid c...
Source: Metabolomics - January 16, 2016 Category: Biology Source Type: research

Metabolomics of chronic obstructive pulmonary disease and obstructive sleep apnea syndrome: response to Maniscalco and Motta
(Source: Metabolomics)
Source: Metabolomics - January 14, 2016 Category: Biology Source Type: research

Identification of urinary biomarkers after consumption of sea buckthorn and strawberry, by untargeted LC–MS metabolomics: a meal study in adult men
Abstract Berries may improve health; however, accurate assessment of berry intake is still problematic. The discovery of objective biomarkers for intake of berries is therefore important in assessing both intake and compliance. We aimed to identify urinary exposure markers of two very different berries, strawberry and sea buckthorn, in humans. A randomized controlled single-blinded three-way cross-over meal study was conducted in 16 overweight men. The intervention meals consisted of sea buckthorn puree, strawberry puree or an iso-caloric control drink. Urine samples were collected on each test day at t =&nb...
Source: Metabolomics - January 13, 2016 Category: Biology Source Type: research

Urinary metabolomic profiling to identify biomarkers of a flavonoid-rich and flavonoid-poor fruits and vegetables diet in adults: the FLAVURS trial
Abstract The present study aims to investigate the dose dependent effects of consuming diets enriched in flavonoid-rich and flavonoid-poor fruits and vegetables on the urine metabolome of adults who had a ≥1.5 fold increased risk of cardiovascular diseases. A single-blind, dose-dependent, parallel randomized controlled dietary intervention was conducted where volunteers (n = 126) were randomly assigned to one of three diets: high flavonoid diet, low flavonoid diet or habitual diet as a control for 18 weeks. High resolution LC–MS untargeted metabolomics with minimal sample cleanup was perfo...
Source: Metabolomics - January 13, 2016 Category: Biology Source Type: research

Impact of heat stress during seed development on soybean seed metabolome
Abstract Seed development is a temperature-sensitive process that is much more vulnerable than vegetative tissues to abiotic stresses. High temperatures during soybean (Glycine max) seed development frequently results in seed with poor germination, increased incidence of pathogen infection, and decreased economic value. Climate change is expected to increase the incidence and severity of summer heatwaves, and the impact of heat stress on seed development will become more widespread during the course of the twenty-first century. Global metabolite profiles were contrasted between seed from heat-tolerant and heat-su...
Source: Metabolomics - January 8, 2016 Category: Biology Source Type: research

Metabolomics of chronic obstructive pulmonary disease and obstructive sleep apnea syndrome: a comment
(Source: Metabolomics)
Source: Metabolomics - January 8, 2016 Category: Biology Source Type: research