Comprehensive metabolite profiling of onion bulbs ( Allium cepa ) using liquid chromatography coupled with electrospray ionization quadrupole time-of-flight mass spectrometry
ConclusionAs exemplarily demonstrated by comparative metabolic analysis of six onion cultivars the established analytical workflow in combination with targeted and non-targeted data analysis strategies can be successfully applied for comprehensive metabolite profiling of onion bulbs. (Source: Metabolomics)
Source: Metabolomics - February 17, 2017 Category: Biology Source Type: research

Evaluation of metabolomic changes during neoadjuvant chemotherapy combined with bevacizumab in breast cancer using MR spectroscopy
This study aimed to use magnetic resonance (MR) spectroscopy on breast tumor tissue to explore the effect of neoadjuvant therapy on metabolic profiles, determine metabolic effects of the antiangiogenic drug bevacizumab, and investigate metabolic differences between responders and non-responders.MethodsBreast tumors from 122 patients were profiled using high resolution magic angle spinning MR spectroscopy. All patients received neoadjuvant chemotherapy, and were randomized to receive bevacizumab or not. Tumors were biopsied prior, during, and after treatment.ResultsPrincipal component analysis showed clear metabolic changes...
Source: Metabolomics - February 17, 2017 Category: Biology Source Type: research

NMRProcFlow: a graphical and interactive tool dedicated to 1D spectra processing for NMR-based metabolomics
ConclusionBiologists and NMR spectroscopists can easily interact and develop synergies by visualizing the NMR spectra along with their corresponding experimental-factor levels, thus setting a bridge between experimental design and subsequent statistical analyses. (Source: Metabolomics)
Source: Metabolomics - February 17, 2017 Category: Biology Source Type: research

Metabolic heterogeneity of the normal human brain: multivariate analysis of 1 H MRS in vivo spectra acquired at 3T
ConclusionMultivariate approach to1H MRS data analysis provides an insight into the normal brain biochemistry and is helpful in understanding the regional heterogeneity of the normal brain. Such knowledge is crucial for a proper interpretation of altered metabolic pathways in diseases. (Source: Metabolomics)
Source: Metabolomics - February 16, 2017 Category: Biology Source Type: research

Chemogeography of the red macroalgae Asparagopsis : metabolomics, bioactivity, and relation to invasiveness
ConclusionThe metabolomic approach partly discriminates macroalgal populations from various geographic origins. The production of chemical defenses assessed by the bioactivity assay does not match the macroalgal genetic lineage and seems more driven by the environment. The higher content of chemical defenses in temperateversus tropical populations is not in accordance with the LGH and cannot be related to the invasiveness of the macroalgae. (Source: Metabolomics)
Source: Metabolomics - February 16, 2017 Category: Biology Source Type: research

Metabolic perturbations of postnatal growth restriction and hyperoxia-induced pulmonary hypertension in a bronchopulmonary dysplasia model
ConclusionThe present study highlights the variety of metabolic changes that occur due to PNGR- and hyperoxia-induced PH, identifying numerous metabolites and pathways influenced by treatment-specific or combined effects. The rat model used in this study presents a robust means of uncovering the mechanisms that contribute to the pathology of PH. (Source: Metabolomics)
Source: Metabolomics - February 13, 2017 Category: Biology Source Type: research

Multilevel pharmacokinetics-driven modeling of metabolomics data
ConclusionBayesian multilevel pharmacokinetics-driven modeling in metabolomics may be useful in understanding the data and may constitute a new tool for searching towards potential candidates of disease indicators. (Source: Metabolomics)
Source: Metabolomics - February 8, 2017 Category: Biology Source Type: research

A relative quantitative positive/negative ion switching method for untargeted lipidomics via high resolution LC-MS/MS from any biological source
ConclusionsUsing mouse embryonic fibroblasts, we showed that TSC2−/− KD significantly abrogates lipid biosynthesis and that rapamycin can rescue triglyceride (TG) lipids and we show that SREBP−/− shuts down lipid biosynthesis significantly via mTORC1 signaling pathways. We show that in mouse EGFR driven lung tumors, a large number of TGs and phosphatidylmethanol (PMe) lipids are elevated while some phospholipids (PLs) show some of the largest decrease in lipid levels from ~  2000 identified lipid ions. In addition, we identified more than 1500 unique lipid species from human blood plasma. (Source: Metabolomics)
Source: Metabolomics - February 7, 2017 Category: Biology Source Type: research

Metabolic profiling of Drosophila melanogaster metamorphosis: a new insight into the central metabolic pathways
ConclusionThis study supported the strength of metabolomics in the field of developmental biology. The results provided a general view on the metabolic profile ofDrosophila during metamorphosis, which provides basic 3 knowledge for future metabolomics studies usingDrosophila. (Source: Metabolomics)
Source: Metabolomics - February 6, 2017 Category: Biology Source Type: research

Cinnamon: does it hold its promises in cows? Using non-targeted blood serum metabolomics profiling to test the effects of feeding cinnamon to dairy cows undergoing lactation-induced insulin resistance
ConclusionThe data suggest lipolytic and ketogenic effects of cinnamon supplementation in dairy cows during the transition from late gestation to early lactation. The fatty acid metabolites found elevated in the supplemented cows point towards impaired mitochondrial fatty acid β-oxidation. (Source: Metabolomics)
Source: Metabolomics - February 3, 2017 Category: Biology Source Type: research

An untargeted metabolomics method for archived newborn dried blood spots in epidemiologic studies
ConclusionsThis untargeted workflow can be used for analysis of small molecules in archived DBS to discover novel biomarkers, to provide insights into the initiation and progression of diseases, and to provide guidance for disease prevention. (Source: Metabolomics)
Source: Metabolomics - February 3, 2017 Category: Biology Source Type: research

Variation of the microbiota and metabolome along the canine gastrointestinal tract
ConclusionThe microbiota and metabolome vary significantly at different sites along the canine gastrointestinal tract. (Source: Metabolomics)
Source: Metabolomics - February 2, 2017 Category: Biology Source Type: research

The translation of lipid profiles to nutritional biomarkers in the study of infant metabolism
ConclusionFrom this study, we were able to identify and validate three lipids: PC(35:2), SM(36:2) and SM(39:1) that can be used collectively as biomarkers for infant nutrition during early development. These biomarkers can be used to determine whether young infants (3 –6 months) are breast-fed or receive formula milk. (Source: Metabolomics)
Source: Metabolomics - January 28, 2017 Category: Biology Source Type: research

Furosemide enhances the sensitivity of urinary metabolomics for assessment of kidney function
AbstractIntroductionThe ability of urinary metabolomics to detect meaningful, tissue-specific, biological effects (i.e., toxicity, disease) is compounded by high background variability. We hypothesize that sensitivity can be enhanced by imposing a tissue-targeted metabolic stressor.ObjectiveWe tested whether the sensitivity of metabolomics to assess kidney function is improved under the diuretic stress of furosemide.MethodsTo mildly compromise kidney, rats were given a sub-acute dose ofd-serine. Then at 24  h postdose, we administered vehicle solution (control) or the diuretic drug, furosemide, and conducted NMR-based...
Source: Metabolomics - January 21, 2017 Category: Biology Source Type: research

Retinal metabolic events in preconditioning light stress as revealed by wide-spectrum targeted metabolomics
ConclusionsBesides retinal sexual metabolic dimorphism, this study shows that preconditioning is mostly associated with re-organisation of lipid metabolism and changes in amino acid composition, likely reflecting the involvement of arginine-dependent NO signalling. (Source: Metabolomics)
Source: Metabolomics - January 20, 2017 Category: Biology Source Type: research

Candidate serum metabolite biomarkers for differentiating gastroesophageal reflux disease, Barrett ’s esophagus, and high-grade dysplasia/esophageal adenocarcinoma
AbstractIntroduction/objectivesIncidence of esophageal adenocarcinoma (EA), an often fatal cancer, has increased sharply over recent decades. Several important risk factors (reflux, obesity, smoking) have been identified for EA and its precursor, Barrett ’s esophagus (BE), but a key challenge remains in identifying individuals at highest risk, since most with reflux do not develop BE, and most with BE do not progress to cancer. Metabolomics represents an emerging approach for identifying novel biomarkers associated with cancer development.MethodsWe used targeted liquid chromatography-mass spectrometry (LC-MS) to prof...
Source: Metabolomics - January 20, 2017 Category: Biology Source Type: research

Prepartal overfeeding alters the lipidomic profiles in the liver and the adipose tissue of transition dairy cows
ConclusionPrepartal overfeeding significantly altered the concentrations of various sphingolipids, phospholipids, and lysophospholipids in the liver and AT of dairy cows during the periparturient period. (Source: Metabolomics)
Source: Metabolomics - January 18, 2017 Category: Biology Source Type: research

Detection of adulteration in Iranian saffron samples by 1 H NMR spectroscopy and multivariate data analysis techniques
ConclusionThe present study demonstrates that the application of1H NMR spectroscopy coupled with multivariate data analysis is a suitable approach for detection of adulteration in saffron specimens. Outstanding sensitivity and specificity of the PLS-DA model in discriminating the authentic from adulterated samples in external validation confirmed the high predictive power of the model. The advantage of the present method is its power for detecting a wide spectrum of adulterants, ranging from synthetic dyes to herbal materials, in a single assay. (Source: Metabolomics)
Source: Metabolomics - January 18, 2017 Category: Biology Source Type: research

NMR-based metabolomics of transgenic and non-transgenic sweet orange reveals different responses in primary metabolism during citrus canker development
ConclusionAn NMR-based metabolomic approach was useful for understanding plant –pathogen interactions in citrus canker. Our findings provide valuable preliminary insights into different stages of citrus canker development. (Source: Metabolomics)
Source: Metabolomics - January 18, 2017 Category: Biology Source Type: research

Volatile metabolic diversity of Klebsiella pneumoniae in nutrient-replete conditions
ConclusionK. pneumoniae produces a core suite of volatile compounds across all growth media studied, although the volatile metabolic signature of this organism is fundamentally media-dependent. (Source: Metabolomics)
Source: Metabolomics - January 12, 2017 Category: Biology Source Type: research

1 H NMR-based metabolomics approach reveals metabolic alterations in response to dietary imbalances in Megalobrama amblycephala
ConclusionThese results provide an important insight into the diet-affected metabolic disorders that often lead to financial losses in the aquaculture ofMegalobrama amblycephala.Graphical Abstract (Source: Metabolomics)
Source: Metabolomics - January 10, 2017 Category: Biology Source Type: research

Metabolomic analysis of the mechanism of action of yerba mate aqueous extract on Salmonella enterica serovar Typhimurium
ConclusionThis work provides the first evaluation of the mechanism of action of yerba mate extract onS. Typhimurium, revealing a major impact on central carbon metabolism, catalase activity, and possible metabolic links to interference in energy production and membrane integrity. The putative identification of the antibacterial compound yohimbine and the many unidentified compounds provides additional avenues for future investigations of yerba mate compounds capable of traversing or binding toS. Typhimurium ’s membrane. (Source: Metabolomics)
Source: Metabolomics - January 9, 2017 Category: Biology Source Type: research

Ultra high performance liquid chromatography –high resolution mass spectrometry plasma lipidomics can distinguish between canine breeds despite uncontrolled environmental variability and non-standardized diets
ConclusionBy using a phenotypically diverse home-based canine population, we were able to show that high accurate mass lipidomics can enable identification of metabolites in the first pass plasma profile, capturing distinct metabolomic variability associated with genetic differences, despite environmental and dietary variability. (Source: Metabolomics)
Source: Metabolomics - January 5, 2017 Category: Biology Source Type: research

Metabolic derangements identified through untargeted metabolomics in a cross-sectional study of Nigerian children with severe acute malnutrition
AbstractIntroductionSevere acute malnutrition (SAM) is a major cause of child mortality worldwide, however the pathogenesis of SAM remains poorly understood. Recent studies have uncovered an altered gut microbiota composition in children with SAM, suggesting a role for microbes in the pathogenesis of malnutrition.ObjectivesTo elucidate the metabolic consequences of SAM and whether these changes are associated with changes in gut microbiota composition.MethodsWe applied an untargeted multi-platform metabolomics approach [gas chromatography –mass spectrometry (GC-MS) and liquid chromatography–mass spectrometry (L...
Source: Metabolomics - December 27, 2016 Category: Biology Source Type: research

MASTR-MS: a web-based collaborative laboratory information management system (LIMS) for metabolomics
ConclusionMASTR-MS is a comprehensive LIMS solution specifically designed for metabolomics. It captures the entire lifecycle of a sample starting from project and experiment design to sample analysis, data capture and storage. It acts as an electronic notebook, facilitating project management within a single laboratory or a multi-node collaborative environment. This software is being developed in close consultation with members of the metabolomics research community. It is freely available under the GNU GPL v3 licence and can be accessed from,https://muccg.github.io/mastr-ms/. (Source: Metabolomics)
Source: Metabolomics - December 27, 2016 Category: Biology Source Type: research

Computational tools and workflows in metabolomics: An international survey highlights the opportunity for harmonisation through Galaxy
(Source: Metabolomics)
Source: Metabolomics - December 27, 2016 Category: Biology Source Type: research

Pharmacometabolomic signature links simvastatin therapy and insulin resistance
ConclusionWithin this short 6  weeks study, some patients became hyperglycemic and/or insulin resistant. Diabetic markers were associated with decarboxylated small aminated metabolites as well as a branched chain amino acid directly linked to glucose metabolism and fatty acid biosynthesis. Pharmacometabolomics provides powerful tools for precision medicine by predicting development of drug adverse effects in sub populations of patients. Metabolic profiling prior to start of drug therapy may empower physicians with critical information when prescribing medication and determining prognosis. (Source: Metabolomics)
Source: Metabolomics - December 23, 2016 Category: Biology Source Type: research

A clustering-based preprocessing method for the elimination of unwanted residuals in metabolomic data
ConclusionThe CURE method can effectively remove unwanted data variation, and can serve as an alternative UVE method for metabolomic data. (Source: Metabolomics)
Source: Metabolomics - December 20, 2016 Category: Biology Source Type: research

Integration of pharmacometabolomics with pharmacokinetics and pharmacodynamics: towards personalized drug therapy
AbstractPersonalized medicine, in modern drug therapy, aims at a tailored drug treatment accounting for inter-individual variations in drug pharmacology to treat individuals effectively and safely. The inter-individual variability in drug response upon drug administration is caused by the interplay between drug pharmacology and the patients ’ (patho)physiological status. Individual variations in (patho)physiological status may result from genetic polymorphisms, environmental factors (including current/past treatments), demographic characteristics, and disease related factors. Identification and quantification of pred...
Source: Metabolomics - December 19, 2016 Category: Biology Source Type: research

Integration of metabolomic and transcriptomic networks in pregnant women reveals biological pathways and predictive signatures associated with preeclampsia
This study, nested within the Vitamin D Antenatal Asthma Reduction Trial (VDAART), aimed to develop integrated omics models of preeclampsia that have utility in both prediction and in the elucidation of underlying biological mechanisms.MethodsMetabolomic profiling was performed on first trimester plasma samples of 47 pregnant women from VDAART who subsequently developed preeclampsia and 62 controls with healthy pregnancies, using liquid-chromatography tandem mass-spectrometry. Metabolomic profiles were generated based on logistic regression models and assessed using Received Operator Characteristic Curve analysis. These pr...
Source: Metabolomics - December 12, 2016 Category: Biology Source Type: research

The association of fecal microbiota and fecal, blood serum and urine metabolites in myalgic encephalomyelitis/chronic fatigue syndrome
ConclusionIncreased production of SCFA by microbial fermentation in the gut of ME/CFS patients may be associated with deleterious effects on the host energy metabolism. (Source: Metabolomics)
Source: Metabolomics - December 12, 2016 Category: Biology Source Type: research

Targeted analysis of omega-6-derived oxylipins and parent polyunsaturated fatty acids in serum of hepatitis B virus-related hepatocellular carcinoma patients
ConclusionThe study demonstrated LA- and AA-derived oxylipins via the lipoxygenase and cytochrome P450 pathways appeared to be most involved in the pathogenesis of HBV-related HCC. (Source: Metabolomics)
Source: Metabolomics - December 8, 2016 Category: Biology Source Type: research

Metabolomics of the human aqueous humor
ConclusionThe differences between the metabolomic profiles of AH and serum for majority of metabolites can be attributed to the metabolic activity of the ocular tissues leading to the lack or excess of some metabolites, while the high concentration of ascorbate in AH demonstrates the activity of ascorbate-specific pumps at the blood-aqueous border. The post-mortem metabolomic changes are caused by the disruption of the major biochemical cycles and cell lysis. These changes should be taken into account in the analysis of disease-induced changes in post-mortem samples of the ocular tissues. (Source: Metabolomics)
Source: Metabolomics - December 2, 2016 Category: Biology Source Type: research

Granulocyte colony-stimulating factor alters the systemic metabolomic profile in healthy donors
ConclusionOur results show that G-CSF treatment alters biochemical serum profiles, in particular amino acid, lipid and nucleotide metabolism. Additional studies are needed to further evaluate the relevance of these changes in healthy donors. (Source: Metabolomics)
Source: Metabolomics - November 28, 2016 Category: Biology Source Type: research

The degradation of nucleotide triphosphates extracted under boiling ethanol conditions is prevented by the yeast cellular matrix
ConclusionsExtraction of biological samples under boiling ethanol extraction conditions that rapidly inactivate enzyme activity are suitable for labile central energy metabolites such as nucleotide triphosphates due to the stabilizing effect of the yeast matrix. The basis of this phenomenon requires further study.Graphical abstract (Source: Metabolomics)
Source: Metabolomics - November 28, 2016 Category: Biology Source Type: research

Comprehensive quantitative lipidomic approach to investigate serum phospholipid alterations in breast cancer
This study aims to explore five classes of serum phospholipid alterations in breast cancer towards discrimination of breast cancer from benign and healthy controls.MethodsTwenty eight each of breast cancer patients and age-matched benign and healthy control serum samples were used to identify alterations of phospholipids using liquid chromatography-multiple reaction monitoring-mass spectrometry. Both multivariate and univariate statistical analyses were applied to investigate breast cancer associated phospholipid alterations. Differentially expressed phospholipid species were further confirmed by liquid chromatography-tand...
Source: Metabolomics - November 28, 2016 Category: Biology Source Type: research

Stability of targeted metabolite profiles of urine samples under different storage conditions
AbstractIntroductionFew studies have investigated the influence of storage conditions on urine samples and none of them used targeted mass spectrometry (MS).ObjectivesWe investigated the stability of metabolite profiles in urine samples under different storage conditions using targeted metabolomics.MethodsPooled, fasting urine samples were collected and stored at −80 °C (biobank standard), −20 °C (freezer), 4 °C (fridge), ~9 °C (cool pack), and ~20 °C (room temperature) for 0, 2, 8 and 24 h. Metabolite concentrations were quantified with MS using the A...
Source: Metabolomics - November 28, 2016 Category: Biology Source Type: research

Metabolomic analysis of urinary organic acids following intraperitoneal injection with γ-hydroxybutyric acid in rats
In this study, comprehensive organic acid (OA) profiling analysis including OAs related to TCA cycle, GHB and 2-hydroxyglutaric acid (2-HG) as a prominent metabolite was performed by gas chromatography –mass spectrometry (GC–MS) in scan mode to understand the biochemical events and monitor altered OA metabolism in rat urine samples following treatment with GHB.MethodsOA profiling as methoxime/tert-butyldimethylsilyl derivatives by GC –MS combined with star pattern analyses were performed in rat urine samples following intraperitoneal administration of GHB once per day for one (single) and ten consecutive ...
Source: Metabolomics - November 7, 2016 Category: Biology Source Type: research

A novel targeted/untargeted GC-Orbitrap metabolomics methodology applied to Candida albicans and Staphylococcus aureus biofilms
ConclusionEvaluation of the results demonstrates that the Q Exactive GC is suitable for metabolomics analysis using a targeted/untargeted methodology. Many of the results were as expected: e.g. rapid consumption of glucose and fructose from the medium regardless of the cell type. Modulation of sugar-phosphate levels also suggest that the pentose phosphate pathway could be enhanced in the cells from co-cultured biofilms. Untargeted metabolomics results suggested significant production of cell-wall biosynthesis components and the consumption of non-proteinaceous amino-acids. (Source: Metabolomics)
Source: Metabolomics - November 5, 2016 Category: Biology Source Type: research

Metabolomic profiling highlights oxidative damages in platelet concentrates treated for pathogen inactivation and shows protective role of urate
ConclusionOur results indicate oxidative damages occurring at the metabolic level in stored iPCs. Understanding better the role of antioxidants such as urate in ex vivo PCs would definitively provide new insights to ameliorate the storage conditions and preserve the functionality of platelets. (Source: Metabolomics)
Source: Metabolomics - November 4, 2016 Category: Biology Source Type: research

A metabolomic, geographic, and seasonal analysis of the contribution of pollen-derived adenosine to allergic sensitization
ConclusionSugar content and other metabolites may be useful in classifying pollens. Unless other factors create localized exposures that are very different from these models, pollen-derived adenosine is unlikely to be a major factor in allergic sensitization. (Source: Metabolomics)
Source: Metabolomics - November 2, 2016 Category: Biology Source Type: research

Metabolite profiling of developing Camelina sativa seeds
ConclusionThis study resulted in the metabolic profile ofC. sativa during seed development and enabled to identify two novel compounds inCamelina seeds. (Source: Metabolomics)
Source: Metabolomics - October 28, 2016 Category: Biology Source Type: research

Metabolomics profiling of concussion in adolescent male hockey players: a novel diagnostic method
AbstractIntroductionConcussions are a major health concern as they cause significant acute symptoms and in some athletes, long-term neurologic dysfunction. Diagnosis of concussion can be difficult, as are the decisions to stop play.ObjectiveTo determine if concussions in adolescent male hockey players could be diagnosed using plasma metabolomics profiling.MethodsPlasma was obtained from 12 concussed and 17 non-concussed athletes, and assayed for 174 metabolites with proton nuclear magnetic resonance and direct injection liquid chromatography tandem mass spectrometry. Data were analysed with multivariate statistical analysi...
Source: Metabolomics - October 28, 2016 Category: Biology Source Type: research

Mid- and long-term correlations of plasma metabolite concentrations measured by a targeted metabolomics approach
AbstractIntroductionPlasma metabolites measured by metabolomics techniques have been shown to be associated with chronic disease risk in epidemiological studies. However, in most prospective studies metabolomic profiles can only be obtained at a single time point and data on intra-individual variation in metabolite levels over time are sparse.ObjectivesHere, we evaluated the intra-individual variation in the concentrations of 177 metabolites over time using repeat blood samples of 104 adults (50% female).MethodsBlood samples were obtained at a baseline visit between 1994 and 1998 and during two further examinations 14 and ...
Source: Metabolomics - October 26, 2016 Category: Biology Source Type: research

Comprehensive optimization of LC –MS metabolomics methods using design of experiments (COLMeD)
AbstractIntroductionBoth reverse-phase and HILIC chemistries are deployed for liquid-chromatography mass spectrometry (LC –MS) metabolomics analyses, however HILIC methods lag behind reverse-phase methods in reproducibility and versatility. Comprehensive metabolomics analysis is additionally complicated by the physiochemical diversity of metabolites and array of tunable analytical parameters.ObjectiveOur aim was to rationally and efficiently design complementary HILIC-based polar metabolomics methods on multiple instruments using design of experiments (DoE).MethodsWe iteratively tuned LC and MS conditions on ion-swit...
Source: Metabolomics - October 24, 2016 Category: Biology Source Type: research

Circulating sterols as predictors of early allograft dysfunction and clinical outcome in patients undergoing liver transplantation
ConclusionLow ratios of circulating esterified sterols are associated with a high risk of EAD and impaired clinical outcome in the early postoperative phase following LTX. (Source: Metabolomics)
Source: Metabolomics - October 24, 2016 Category: Biology Source Type: research

Weight loss and weight maintenance obtained with or without GLP-1 analogue treatment decrease branched chain amino acid levels
AbstractIntroductionIncreased levels of circulating branched chain amino acids (BCAAs), as well as phenylalanine, and tyrosine have been suggested to be involved in the pathogenesis of insulin resistance and type 2 diabetes. However, it is unknown how these metabolites are affected by weight loss, and during weight-maintaining treatment with glucagon-like peptide-1 receptor agonist (GLP-1 RA).ObjectiveWe aimed to characterize changes in metabolites related to protein turnover and glycolysis after a weight loss intervention followed by long term weight maintenance with/without GLP-1 RA.MethodsFifty-eight obese individuals u...
Source: Metabolomics - October 22, 2016 Category: Biology Source Type: research

Erratum to: KLK1 and ZG16B proteins and arginine –proline metabolism identified as novel targets to monitor atherosclerosis, acute coronary syndrome and recovery
(Source: Metabolomics)
Source: Metabolomics - October 5, 2016 Category: Biology Source Type: research

Intensive determination of storage condition effects on human plasma metabolomics
ConclusionA large volume of data regarding storage stability was obtained. These data are a contribution to the discovery of biomarker candidates without misselection based on unreliable values and to the establishment of suitable handling procedures for targeted biomarker quantification. (Source: Metabolomics)
Source: Metabolomics - September 30, 2016 Category: Biology Source Type: research

Identification of putative biomarkers for type 2 diabetes using metabolomics in the Korea Association REsource (KARE) cohort
AbstractIntroductionType 2 diabetes (T2D) is a multifactorial disease resulting from a complex interaction between environmental and genetic risk factors. Metabolomics provide a logical framework that reflects the functional endpoints of biological processes being triggered by genetic information and various external influences.ObjectivesIdentification of metabolite biomarkers can shed insight into etiological pathways and improve the prediction of disease risk. Here, we aimed to identify serum metabolites as putative biomarkers for T2D and their association with genetic variants in the Korean population.MethodsA targeted ...
Source: Metabolomics - September 28, 2016 Category: Biology Source Type: research