Lack of association between sirtuin gene variants and endometrial cancer
This study took paraffin-embedded endometrium specimens from 48 endometrial cancer patients and 53 healthy control subjects. SIRT1 gene variants were determined with the PCR-CCTP method. The result of the single-nucleotide polymorphism (SNP) analysis indicated no significant relationship with endometrial cancer. Recent studies have shown that SIRT has had a positive effect on endometrial tumors. Our study analyzed, for the first time, the association between SIRT1 gene polymorphisms and endometrial cancer. Because of the low population our results are not reflecting the exact contribution of the polymorphism to the develop...
Source: Meta Gene - October 26, 2018 Category: Genetics & Stem Cells Source Type: research

Investigation of antibiotic resistance and the presence of integron genes among ESBL producing Klebsiella isolates
This study aimed to determine the prevalence of antibiotic resistance pattern, and class 1–3 integrons in clinical isolates of Klebsiella among Iranian patients. From March to September 2016, plasmid DNA of 169 Klebsiella isolates was extracted after determining their resistance pattern and ESBL production by disk diffusion method. The presence of class 1–3 integrons was detected by targeting integrase genes with polymerase chain reaction (PCR) method. Out of 169 Klebsiella, 41.1% of Klebsiella pneumoniae and 18.2% of Klebsiella oxytoca isolates were ESBL-positive. The most resistance rate in both species was a...
Source: Meta Gene - October 26, 2018 Category: Genetics & Stem Cells Source Type: research

Association of interleukin-6 rs1800796 polymorphism with reduced cognitive performance in healthy older adults
Publication date: Available online 25 October 2018Source: Meta GeneAuthor(s): Natalia Ewa Bezuch, Steven Bradburn, Sarianna Sipilä, Mati Pääsuke, Helena Gapeyeva, Andrea B. Maier, Jean-Yves Hogrel, Yoann Barnouin, Gillian Butler-Browne, Marco Narici, Jamie McPhee, Chris MurgatroydAbstractWith increasing life expectancy, age-associated cognitive impairment is an escalating problem worldwide. Inflammation is one of the features that characterises cognitive decline and can stimulate neurodegeneration. Interleukin 6 (IL-6) is a cytokine frequently associated with a pro-inflammatory phenotype and increased levels...
Source: Meta Gene - October 25, 2018 Category: Genetics & Stem Cells Source Type: research

Significant associations between prolactin gene polymorphisms and growth traits in the channel catfish (Ictalurus punctatus Rafinesque, 1818) core breeding population
Publication date: Available online 18 October 2018Source: Meta GeneAuthor(s): Shiyong Zhang, Xiang Li, Xiaohui Chen, Jianlin Pan, Minghua Wang, Liqiang Zhong, Qin Qin, Wenji BianAbstractThe aim of this study was to analyze the effects of the prolactin gene (PRL) on growth traits in channel catfish (Ictalurus punctatus). We sequenced pooled DNA samples from channel catfish to identify single nucleotide polymorphisms (SNPs) in the PRL gene. The identified SNPs were then genotyped in 176 individuals from the G1 core breeding population of catfish using the multiplex SNaPshot method. SNP genotypes and diplotypes of the PRL gen...
Source: Meta Gene - October 20, 2018 Category: Genetics & Stem Cells Source Type: research

Definition of an oxidative stress status by combined assessment of Malondialdehyde and Oxidized-LDL: A study in patients with type2 diabetes and control
ConclusionsWe attempted to investigate applicability of MDA and ox-LDL in predicting T2DM related oxidative stress. Present data strongly suggest that using both of MDA and ox-LDL increases their specificity in predicting oxidative milieu and diabetic nephropathy. Moreover, we showed that HDL levels above 35 mg/dl might have beneficial effect on oxidative reactions. (Source: Meta Gene)
Source: Meta Gene - October 17, 2018 Category: Genetics & Stem Cells Source Type: research

Genetic association study of C5178A and G10398A mitochondrial DNA variants with type 2 diabetes in Bangladeshi population
This study aims to explore the relationship of mtDNA C5178A and G10398A variations with T2D in Bangladeshi population. A total of 249 unrelated Bangladeshi populations (127 T2D and 122 healthy controls) were enrolled in this study. Specific DNA sequences within mitochondria were amplified by PCR followed by digestion at the polymorphic sites using AluI and DdeI restriction enzymes. Analyses revealed that 66.93% T2D and 59.02% healthy individuals carried the major allele ‘G’ at 10398 position of the mtGenome; while the minor allele ‘A’ was present in 33.07% T2D and 40.98% healthy individuals. G10398A...
Source: Meta Gene - October 16, 2018 Category: Genetics & Stem Cells Source Type: research

A novel allele frequency trajectories template to discriminate genetic similarity among populations
Publication date: Available online 13 October 2018Source: Meta GeneAuthor(s): Sagy Elzalabany, Tarek Taha, Sahar Fawzi, Olfat ShakerAbstractPopulations' allele frequency studies are one of the major topics of Population genetics. In this research, an Allele Frequency Quality Control System (AFQCS) is proposed as a verification tool for populations' genetics data. This system visualizes and analyses statistically the allele frequencies of 15 Short Tandem Repeats (STRs) autosomal loci based on 65 previously published population genetics studies. Allele Frequency Trajectories (AFTs) for all addressed populations were plotted....
Source: Meta Gene - October 13, 2018 Category: Genetics & Stem Cells Source Type: research

Evidence for an autosomal recessive pattern of inheritance in Keratitis-ichthyosis-deafness (KID) syndrome: Exome sequencing reveals a novel homozygous GJB2 mutation
We present evidence that KID syndrome can be caused by a homozygous GJB2 mutation and demonstrate an autosomal recessive mode of inheritance for the first time at a molecular level.The spectrum of skin manifestations associated with gap junction gene mutations is still growing, particularly in the case of Cx26. Our findings add to the list of pathogenic variants associated with this condition, and contribute to an insight into the genotype-phenotype correlations of Cx26 mutations, thus elucidating the function of different regions of the protein and further add to the widely diverging associated phenotypes. (Source: Meta Gene)
Source: Meta Gene - October 11, 2018 Category: Genetics & Stem Cells Source Type: research

Are long non-coding RNAs involved in the interaction circuit between estrogen receptor and vitamin D receptor?
Publication date: Available online 5 October 2018Source: Meta GeneAuthor(s): Vahid Kholghi Oskooei, Soudeh Ghafouri-FardAbstractBreast cancer as a molecularly heterogeneous disorder harbors dysregulation of several signaling pathways. Estrogen receptor (ER) and vitamin D receptor (VDR) are tow signaling pathways whose fundamental roles in breast cancer have been documented. The presence of a negative vitamin D response element in the Estrogen receptor 1 (ESR1) gene promoter has provided evidences for interactions between ER and VDR signaling pathways. Moreover, recent data indicate that certain long non-coding RNAs (lncRNA...
Source: Meta Gene - October 7, 2018 Category: Genetics & Stem Cells Source Type: research

Random regression models to explore genetic variation and genetic variability in the growth curve of Baluchi lambs
Publication date: Available online 1 October 2018Source: Meta GeneAuthor(s): Farhad Ghafouri-Kesbi, Mohsen GholizadehAbstractThe aim was to apply Random Regression Model (RRM) to describe growth curve of Baluchi lambs. Data was retrieved from an experimental population of Baluchi sheep and consisted of body weight records from 50 to 400 days of age. Regressions for the direct and maternal random effects in the RRM were modeled using different combinations of legendre polynomials with orders from one (i.e., simple repeatability model, SRM) to four. Mean trends were also modeled through a quadratic regression on orthogonal...
Source: Meta Gene - October 5, 2018 Category: Genetics & Stem Cells Source Type: research

Isolation and identification of culturable bacteria from honeydew of Indian lac insect, Kerria lacca (Kerr) (Hemiptera: Tachardiidae)
Publication date: Available online 1 October 2018Source: Meta GeneAuthor(s): Gulsaz Shamim, Kewal Krishan Sharma, Ranganathan RamaniAbstractIndian lac insect, Kerria lacca belongs to a distinct group of phytosuccivorous insects feeding on sugar-rich phloem sap and excreting excess sugar in the form of large quantities of honeydew, which serves as an excellent medium for microbial growth. This work has made a novel attempt to isolate and identify culturable bacterial species associated with lac insect honeydew, which can be further used to unravel their significance by identifying and characterizing semiochemicals or bacter...
Source: Meta Gene - October 5, 2018 Category: Genetics & Stem Cells Source Type: research

Significance of CCL2 (−2518A/G), CCR2 (190G/A) and TLR4 polymorphisms (896 A/G and 1196C/T) in tuberculosis risk in Indian population
Publication date: December 2018Source: Meta Gene, Volume 18Author(s): Paras Singh, Roopali Rajput, Rohit Sarin, Devika TayalAbstractTuberculosis remains one of the major health burden worldwide; with substantial mortality in Indian population. Several host gene polymorphisms have been studied wherein association between variations in host genes and susceptibility or resistance to tuberculosis have been reported. In the present study, we aimed at investigating the effect of polymorphisms of CCL2, CCR2 and TLR4 genes on risk of acquiring tuberculosis in Indian population. The CCL2 (−2518A/G) and TLR4 (D299G or 896A/G &...
Source: Meta Gene - October 5, 2018 Category: Genetics & Stem Cells Source Type: research

5-bp insertion/deletion polymorphism in the promoter region of LncRNA GAS5 and cancer risk: A meta-analysis of 7005 cases and 8576 controls
Publication date: December 2018Source: Meta Gene, Volume 18Author(s): Mohammad Hashemi, Abdolkarim Moazeni-Roodi, Farhad Tabasi, Saeid GhavamiAbstractSeveral studies investigated the impact of 5-bp insertion/deletion (ins/del) polymorphism in the promoter region of GAS5 LncRNA on cancer risk and the results were inconsistent. So, we performed a meta-analysis to examine the association between GAS5 5-bp ins/del polymorphism and cancer risk. The PubMed, Web of Science, Scopus, and Google scholar databases were searched and 8 relevant studies including 7005 cases and 8576 controls participants were identified. Pooled odds rat...
Source: Meta Gene - October 5, 2018 Category: Genetics & Stem Cells Source Type: research

A novel missense mutation of the HGD gene causes Alkaptonuria
Publication date: December 2018Source: Meta Gene, Volume 18Author(s): Shahab Noorian, Bahareh Dehghan Banadaki, Arya Sotoudeh, Shahram Savad, Mohammad Hossein ModarressiAbstractAlkaptonuria (AKU) is a rare autosomal recessive disease that leads to metabolic disorder, characterized by accumulation of homogentisic acid which is related to HGD gene mutations. In this article, we report the case of a 13-year-old male and his sibling with clinical symptoms of AKU who referred to the clinic. The next generation sequencing of the HGD gene was performed and the detected mutation was confirmed by Sanger sequencing. The results indi...
Source: Meta Gene - October 5, 2018 Category: Genetics & Stem Cells Source Type: research

The association between ACE I/D polymorphism and the risk of Alzheimer's disease in Lebanon
In conclusion, our study suggests that ACE I allele and female gender are associated with increased risk of AD in the Lebanese population. (Source: Meta Gene)
Source: Meta Gene - October 5, 2018 Category: Genetics & Stem Cells Source Type: research

Effect of hypermethylation in ovarian cancer: Computational approach
Publication date: Available online 12 September 2018Source: Meta GeneAuthor(s): Imane Saif, Najwa Hassou, Karam Allali, Moulay Mustapha EnnajiAbstractOvarian cancer in women represents a mortality rate of>4% worldwide. In cancer, CpG islet hypermethylation of some gene promoters often leads to the inactivation of tumor suppressor genes. Ovarian cancer is characterized by a targeted hypermethylation, hence the need to study this hypermethylation. We have adopted the hypothesis that a very high mutation rate can cause the epimutation phenomenon, causing a 5-fold deamination methyl-cytosine in thymine, consequence of the m...
Source: Meta Gene - September 13, 2018 Category: Genetics & Stem Cells Source Type: research

Analysis of occurrence of MTRR gene polymorphism in down syndrome and other intellectually disabled children
Publication date: Available online 12 September 2018Source: Meta GeneAuthor(s): Avani Patel, Mansi Desai, J.B. ChauhanAbstractThe 5-methylenetetrahydrofolate homocysteine methyltransferase reductase (MTRR) is a key enzyme involved in folate metabolism. Defect in folate metabolism can lead to abnormal DNA synthesis, repair and methylation which lead to abnormal DNA replication and segregation. The most common polymorphism of MTRR genec.66A > G was analyzed in 30 Down syndrome (DS), 30 intellectually disabled (ID) and 60 control children of Gujarat using PCR-RFLP. The results of the present study revealed negative ass...
Source: Meta Gene - September 12, 2018 Category: Genetics & Stem Cells Source Type: research

Identification of potential antibiotic targets in the proteome of multi-drug resistant Proteus mirabilis
Publication date: Available online 10 September 2018Source: Meta GeneAuthor(s): Nosheen Ehsan, Sajjad Ahmad, Afifa Navid, Syed Sikander AzamAbstractProteus mirabilis is pathogen of the urinary tract and is resistant to many of the clinically used antibiotics. Computational identification of novel antibiotic targets could not only save time but also in parallel provide an array of opportunities for developing novel antibiotics to treat infections caused by this pathogen. In the current study, a step-wise subtraction of P. mirabilis proteome was achieved in search of potential antibiotic targets. The main objectives were to ...
Source: Meta Gene - September 11, 2018 Category: Genetics & Stem Cells Source Type: research

Gender-specific effects of MMP-2 and MMP-9 gene variants and the risk of aneurysmal subarachnoid haemorrhage
In this study, we have evaluated the association between MMP gene variants and aneurysmal subarachnoid hemorrhage (aSAH). The rs243865 in MMP-2 and rs17576 in MMP-9 genes were genotyped using Taqman allelic discrimination assay. Upon gender stratification, the presence of G allele of MMP-9 gene exon variant was found to increase the risk of aSAH by 1.4 fold (p = 0.032), in men. The GG genotype also showed a trend towards association with the risk of aSAH (OR: 1.785, CI: 0.971–3.283, p = 0.062) in men, after adjusting for the vascular risk factors. In women, no association was observed. The MMP-2 gene variants...
Source: Meta Gene - September 8, 2018 Category: Genetics & Stem Cells Source Type: research

IL27 gene single nucleotide polymorphisms confer susceptibility to rheumatoid arthritis in Iranian population
ConclusionPresent findings indicated that IL27 rs181206 polymorphism was involved in susceptibility to RA in the Iranian population. (Source: Meta Gene)
Source: Meta Gene - September 8, 2018 Category: Genetics & Stem Cells Source Type: research

Expression analysis of apoptosis-related genes in bladder cancer patients
ConclusionExpression analysis of these genes in tumor or urine is a tool for detection of BC. (Source: Meta Gene)
Source: Meta Gene - September 1, 2018 Category: Genetics & Stem Cells Source Type: research

A novel compound heterozygote mutation in the ARSB gene in a patient with Maroteaux-Lamy syndrome and its Insilico evaluation
ConclusionThese findings implicate the compound heterozygote mutation in ARSB gene and could be important for prenatal diagnosis. This is the first study and the first compound heterozygote that we report in this region of Asia. (Source: Meta Gene)
Source: Meta Gene - August 30, 2018 Category: Genetics & Stem Cells Source Type: research

Ehlers-Danlos Syndrome classical type: A novel COL5A2 missense mutation with possible additive effect of a COL5A1 stop-gain mutation in a strongly correlated phenotype
Publication date: Available online 28 August 2018Source: Meta GeneAuthor(s): Francesca Cortini, Chiara Villa, Barbara Marinelli, Sara Franchetti, Manuela Seia, Angela Cecilia Pesatori, Nicola Montano, Alessandra BassottiAbstractClassical Ehlers-Danlos syndrome (cEDS) is a heritable connective tissue disorder (HCTD) characterized by skin hyperelasticity, joint hypermobility and general tissue fragility. Mutations in COL5A1 and COL5A2 genes, encoding the type V collagen proalpha-1 (pro-α1) and proalpha-2 (pro-α2) chains respectively, are responsible of approximately 90% cEDS patients. The molecular basis of cEDS ...
Source: Meta Gene - August 29, 2018 Category: Genetics & Stem Cells Source Type: research

Development and characterization of Novel Microsatellite markers in great snakehead, Channa marulia (Hamilton, 1822)
Publication date: Available online 28 August 2018Source: Meta GeneAuthor(s): Abhinav Pathak, Rajeev K. Singh, Vindhya Mohindra, Kuldeep K. Lal, Anindya S. Barman, J.K. JenaAbstractThe species-specific microsatellite markers in the great snakehead, Channa marulia, were mined from the genome, through an enriched genomic library construction. Total 27 microsatellite loci exhibited polymorphism. The repeat containing sequences were annotated to ensure the genetic neutrality of the loci. Nineteen, consistently scorable loci were validated, and used to estimate the genetic indices in n = 67 individuals sampled from three Ind...
Source: Meta Gene - August 28, 2018 Category: Genetics & Stem Cells Source Type: research

Association of tumor necrosis factor-alpha gene promoter polymorphism and its mRNA expression level in coronary artery disease
Publication date: Available online 25 August 2018Source: Meta GeneAuthor(s): Parham Nejati, Samaneh Naeimipour, Aref Salehi, Majid ShahbaziAbstractBackgroundumor necrosis factor-alpha (TNF-α) is a pro-inflammatory cytokine that may play an important role in inflammatory diseases like coronary artery disease (CAD). As a result of controversy on the impact of polymorphisms in the promoter region of TNF-α gene on transcription, this case control study was designed to evaluate the effects of TNF-α -308G > A (rs1800629) and TNF-α − 238G > A (rs361525) on mRNA expression level and ...
Source: Meta Gene - August 25, 2018 Category: Genetics & Stem Cells Source Type: research

Therapeutic effects of Spirulina against experimentally-induced non-alcoholic fatty liver in rats may involve miR-21, -34a and -122
Conclusion: Spirulina treatment of HFD-NAFL in rats modulated glucose, insulin sensitivity and showed anti-steatotic properties; effects that could be attributed at least partially to modulating hepatic microRNA expression. In conclusion, Spirulina can be considered as a potential anti-steatotic agent and might provide promising therapeutic approaches in NAFLD. (Source: Meta Gene)
Source: Meta Gene - August 23, 2018 Category: Genetics & Stem Cells Source Type: research

A case report of Duchenne muscular dystrophy; identification of a novel mutation in dystrophin gene using next generation sequencing
Publication date: Available online 23 August 2018Source: Meta GeneAuthor(s): Atieh Teymoori, Mohsen Azimi-Nezhad, Reza Ebrahimzadeh-VesalAbstractDuchenne muscular dystrophy (DMD) is one of the most common form of neuromuscular dystrophy in male children. Mutation in Dystrophin gene is responsible for DMD disease. Here, we aimed to identify the possible causing mutation(s) in a 9-year-old male patient with muscular dystrophy. Multiplex Ligation-dependent Probe Amplification (MLPA) results did not find deletions/duplications mutations. Next generation sequencing (NGS) results indicated a hemizygous nonsense c.3655G > ...
Source: Meta Gene - August 23, 2018 Category: Genetics & Stem Cells Source Type: research

Bioinformatics analysis of a novel Glutaredoxin gene segment from a hot spring metagenomic DNA library
Publication date: Available online 18 August 2018Source: Meta GeneAuthor(s): N. Rawat, A. Shanker, G.K. JoshiAbstractThe present work describes the sequence, phylogenetic and structure analysis of a clone with insert size of 820 nucleotides obtained from the metagenomic library prepared from a hot spring located in the state of Uttarakhand, India. Open Reading Frame analysis revealed a coding sequence of 420 nucleotides. This region encodes a protein of 139 amino acids which depicts homologous relationship with glutaredoxin of Thermus sp. Multiple sequence alignment shows the classic glutaredoxin domain with a redox active...
Source: Meta Gene - August 19, 2018 Category: Genetics & Stem Cells Source Type: research

Genetic stability using RAPD and ISSR markers in efficiently in vitro regenerated plants of Inula royleana DC
Publication date: Available online 18 August 2018Source: Meta GeneAuthor(s): Samar Amin, Tareq A. Wani, Zahoor Ahmad Kaloo, Seema Singh, Riffat John, Umer Majeed, Gowhar Ahmad ShapooAbstractInula royleana (Asteraceae) is a perennial medicinal herb native to Western Himalaya. In Kashmir Himalaya it is commonly known as ‘GugiPhool’. The plant has been traditionally used for various putative health benefits like headache, intestinal problems and blood pressure. The plant is rich source of alkaloids and other metabolites. The important medicinal properties which include antimicrobial, anti-inflammatory, antiprolife...
Source: Meta Gene - August 18, 2018 Category: Genetics & Stem Cells Source Type: research

Assessment of serum level of MiRNAs before and after treatment with sofosbuvir in Egyptian patients with chronic HCV infection
In conclusion, aberrantmiRNA array especially miR-122 and miR-221could potentially extract data for HCV patients in response to combined new therapy. These findings empathizes the importance of contributing the role of miRNAsduring presence and clearance of HCV. (Source: Meta Gene)
Source: Meta Gene - August 16, 2018 Category: Genetics & Stem Cells Source Type: research

Association of the 16q24.3 region gene variants rs1805007 and rs4785763 with heightened risk of melanoma in Latvian population
Publication date: Available online 14 August 2018Source: Meta GeneAuthor(s): Aija Ozola, Dace Ruklisa, Dace PjanovaAbstractChromosome region 16q24.3 has been shown to modify the risk for developing melanoma in genome-wide association studies (GWAS). This region includes at least three SNPs for which significant independent effects on melanoma risk have been demonstrated: rs258322 (CDK10 intron), rs4785763 (pseudogene AFG3L1P), and rs8059973 (flanking 5′UTR of DBNDD1). Also variants within the MC1R gene, located in the same region, are known to be associated with an increased melanoma risk. However, the exact risk the...
Source: Meta Gene - August 14, 2018 Category: Genetics & Stem Cells Source Type: research

Endophytic bacterial community of rice (Oryza sativa L.) from coastal saline zone of West Bengal: 16S rRNA gene based metagenomics approach
This study has enumerated the diversity of endophytic bacteria from rice grown in the saline zone of West Bengal that would help us to design a better strategy for cultivation under abiotic stress condition. (Source: Meta Gene)
Source: Meta Gene - August 14, 2018 Category: Genetics & Stem Cells Source Type: research

Polymorphism analysis in genes associated with meat tenderness in Nelore cattle
Publication date: Available online 10 August 2018Source: Meta GeneAuthor(s): Camila Urbano Braz, Jeremy Francis Taylor, Jared Egan Decker, Tiago Bresolin, Rafael Espigolan, Diogo Anastácio Garcia, Daniel Gustavo Mansan Gordo, Ana Fabrícia Braga Magalhães, Lucia Galvão de Albuquerque, Henrique Nunes de OliveiraAbstractThe aims of this study were to identify haplotyped loci associated with meat tenderness (WBSF) in candidate genes and to search for new polymorphisms in these regions that influence such trait in Nelore cattle. Fifty-two genes that had previously been associated with WBSF or that ha...
Source: Meta Gene - August 11, 2018 Category: Genetics & Stem Cells Source Type: research

Association of TAS2R38 polymorphism with measures of adiposity in Indian population
ConclusionTasting ability and adiposity measures are associated in Indian population. Large scale studies are required for evaluating the association of tasting status on adiposity in India. (Source: Meta Gene)
Source: Meta Gene - August 8, 2018 Category: Genetics & Stem Cells Source Type: research

Adenosine A2A receptor gene polymorphisms (ADORA2A) are associated with maximal concentric contraction pain
ConclusionsADORA2A polymorphisms are moderately associated with muscle pain during maximal contractions in uninjured muscle. (Source: Meta Gene)
Source: Meta Gene - July 28, 2018 Category: Genetics & Stem Cells Source Type: research

A review on effect of genetic features on treatment responses in acute myeloid leukemia
Conclusion: Incorporating this knowledge into the clinical settings is important enough in order to improve the disease outcome and clinical management of patients with AML. (Source: Meta Gene)
Source: Meta Gene - July 28, 2018 Category: Genetics & Stem Cells Source Type: research

Lack of association between the toll-like receptor 4 gene c.896A > G polymorphism and the predisposition to periodontal disease: An updated systematic review and meta-analysis
Publication date: Available online 25 July 2018Source: Meta GeneAuthor(s): Nuno Gonçalves-Anjo, Fátima Leite-Pinheiro, António Cortinhas, Teresa Bento, José Carlos Leitão, Isabel Dias, Carlos Viegas, Estela Bastos (Source: Meta Gene)
Source: Meta Gene - July 25, 2018 Category: Genetics & Stem Cells Source Type: research

Idiopathic premature ovarian failure and its association to the abnormal longitudinal changes of telomere length in a population of Iranian infertile women: A pilot study
This study examines the association between the relative telomere length and idiopathic POF in a group of Iranian women.MethodsThe blood genomic DNA was extracted from 40 idiopathic POF patients (case group) and 40 fertile women (control group). The relative telomere length (RTL) was evaluated by quantitative Real-Time PCR using specific telomeric primers. RTL was calculated as T (telomere)/S (single copy gene) ratio and compared between infertile and fertile groups.ResultsA strong association was considered between telomere size and idiopathic premature ovarian failure. In patients the relative telomere length showed to b...
Source: Meta Gene - July 25, 2018 Category: Genetics & Stem Cells Source Type: research

Synergistic interactions of Angiotensin Converting Enzyme (ACE) gene and Apolipoprotein E (APOE) gene polymorphisms with T1DM susceptibility in south India
Publication date: Available online 25 July 2018Source: Meta GeneAuthor(s): Padma-Malini Ravi, Rathika Chinniah, Ramgopal Sivanadham, Murali Vijayan, Dharmarajan Pannerselvam, S. Pushkala, Balakrishnan KaruppiahAbstractType 1 diabetes mellitus (T1DM) is an autoimmune endocrine disease, in which both gene-gene and gene-environmental factors play a key role in the development and progression of the disease. The main aim of the present study was to determine the association of Angiotensin Converting Enzyme (ACE) and Apolipoprotein E (APOE) gene polymorphisms in T1DM patients. A cohort of 196 T1DM patients (n = 196) and age...
Source: Meta Gene - July 25, 2018 Category: Genetics & Stem Cells Source Type: research

The role of chemerin and vaspin in Egyptian patients with viral hepatitis C
ConclusionThis study investigated and demonstrated that there is correlation between serum chemerin and vaspin levels and stages of fibrosis compared to controls, where the serum chemerin level increases in chronic hepatitis C patients compared to controls and increases with progression of stage of fibrosis. Whereas the serum vaspin level decreases in chronic hepatitis C patients compared to controls and increases with progression of stage of fibrosis to become near the serum levels in controls. (Source: Meta Gene)
Source: Meta Gene - July 25, 2018 Category: Genetics & Stem Cells Source Type: research

CAT-262CT Genotype shows higher catalase activity in seminal plasma and lower risk of male infertility
The objective of this study was to evaluate the correlation between the CAT C-262T polymorphism and male infertility. Semen samples from 400 infertile patients and 80 donors with proven fertility were included in the study. Standart semen parameters, DNA integrity and oxidative stress were evaluated. Genotype frequencies for the CAT C-262T polymorphism were determined by PCR-RFLP. Catalase concentration and activity in seminal plasma were evaluated by western-blot and activity gels respectively while catalase mRNA levels in mature spermatozoa were evaluated by qPCR. The CC genotype was associated with an increased risk of ...
Source: Meta Gene - July 25, 2018 Category: Genetics & Stem Cells Source Type: research

Assessment of the rs2645424 C/T single nucleotide polymorphisms in the FDFT1 gene, hepatic expression, and serum concentration of the FDFT in patients with nonalcoholic fatty liver disease
Publication date: Available online 23 July 2018Source: Meta GeneAuthor(s): Yasar Colak, Ender M. Coskunpinar, Ebubekir Senates, Yasemin Musteri Oltulu, Ilhan Yaylim, Ozlem Kurnaz Gomleksiz, N. Ozan Tiryakioglu, Burcu Hasturk, Cumhur Gokhan Ekmekci, Hulya Yilmaz AydoganAbstractDespite being the most common chronic liver disease, the pathogenesis of nonalcoholic fatty liver disease (NAFLD) still remains unclear. According to the genome-wide association studies (GWAS) alternative alleles of the farnesyl-diphosphate farnesyltransferase 1 (FDFT1) gene involved in cholesterol biosynthetic pathway are known to affect hepatic squa...
Source: Meta Gene - July 24, 2018 Category: Genetics & Stem Cells Source Type: research

Role of myeloperoxidase in hepatitis C virus related hepatocellular carcinoma
This study was conducted on a total number of 59patients. These were subdivided into 25patients of hepatocellular carcinoma and 34 patients of chronic liver diseases (CLDs) with cirrhosis. All studied 59patients were associated with HCV infection. All patients were examined immunohistochemically to demonstrate the expression of MyeloperoxidaseResultsConcerning the expression of Myeloperoxidase in HCC and cirrhoticpatients; the study revealed that more MPO expression was found in HCC cases when compared with cirrhoticpatients (P –value
Source: Meta Gene - July 20, 2018 Category: Genetics & Stem Cells Source Type: research

Identification of S100 calcium binding protein A9 as a prognostic biomarker in gallbladder cancer
Publication date: Available online 11 July 2018Source: Meta GeneAuthor(s): Shushruta Bhunia, Sanjeev Gupta, Braj Raj Shrivastava, Pramod Kumar TiwariAbstractBackgroundGallbladder cancer (GBC) is the most lethal orphan malignancy of biliary tract, with poor prognosis and varied global incidences, predominately reported from, Northern India. Moreover, early diagnostic biomarker(s) of GBC is still undetermined. Earlier studies have demonstrated that S100A9 is associated with the prognosis of various malignancies. S100A9 (calgranulin B or Myeloid Related Protein-14) is a calcium binding protein which has wide range of possible...
Source: Meta Gene - July 11, 2018 Category: Genetics & Stem Cells Source Type: research

Association study between STAT4 polymorphisms and susceptibility to systemic lupus erythematosus disease: A systematic review and meta-analysis
ConclusionsIn summary, this study reported association between rs7574865 and increased risk of SLE, although rs7601754 indicated association with decreased SLE risk. (Source: Meta Gene)
Source: Meta Gene - July 11, 2018 Category: Genetics & Stem Cells Source Type: research

Role of matrix metalloproteinase-9 polymorphisms in basement membrane degradation and pathogenesis of oral submucous fibrosis
Publication date: June 2018Source: Meta Gene, Volume 16Author(s): Atul Katarkar, Chandraday Prodhan, Sanjit Mukherjee, Jay G. Ray, Keya ChaudhuriAbstractOral submucous fibrosis (OSMF) is regarded as a collagen and collagenase metabolic disorder. Aberrant expression of matrix metalloproteinases (especially MMP-9) plays important role in remodeling of extracellular matrix (ECM) during development of OSMF. Single nucleotide polymorphisms (SNPs) in MMP-9 promoter and coding region have been demonstrated to be associated with several diseases. In this case-control study, 196 controls and 189 OSMF patients were genotyped at four...
Source: Meta Gene - July 11, 2018 Category: Genetics & Stem Cells Source Type: research

Editorial Board
Publication date: June 2018Source: Meta Gene, Volume 16Author(s): (Source: Meta Gene)
Source: Meta Gene - July 11, 2018 Category: Genetics & Stem Cells Source Type: research

Association of interleukin-18 gene promoter polymorphisms with coronary artery disease in northern Iranian population
This study investigated the association of the IL-18 gene promoter polymorphisms (− 137 and − 607) with CAD in northern Iranian population.MethodsGenomic DNA was extracted from peripheral blood of 314 patients with CAD and 364 healthy controls by a standard protocol. Sequence-Specific Primer-PCR was used for genotyping. Total RNA was extracted from peripheral blood leukocytes from 80 patients with CAD and 80 healthy individuals using Trizol reagent. Real-time PCR was used to measure the level of IL-18 mRNA in the patients and controls.ResultsAnalysis of genotypes revealed that the frequencies of genot...
Source: Meta Gene - July 11, 2018 Category: Genetics & Stem Cells Source Type: research

Association of Neuregulin-1 gene polymorphisms with neuro-cognitive features of schizophrenia patients from South India: A pilot study
Publication date: June 2018Source: Meta Gene, Volume 16Author(s): Smriti Anand, Amudha Govindaraju, Vinithra Vairavan, Suresh Kumar Narayanan, Rashmi Rajagopal, Anirudh Chellappa, Ashok Ayyappa, Kumanan Thiagarajan, Ananda Krishnan Kumar, GaneshPrasad ArunKumarAbstractNumerous genes have been associated with schizophrenia. In particular, Neuregulin-1 (NRG1) has been widely implicated. Specifically, a 7-marker haplotype called HAPICE in NRG1 is correlated with schizophrenia in many ethnic groups. However, the association of these markers with endophenotypes has shown many contrasting results. India, which harbours one sixth...
Source: Meta Gene - July 11, 2018 Category: Genetics & Stem Cells Source Type: research

Bioenergetic diversity of the human gut microbiome
Publication date: June 2018Source: Meta Gene, Volume 16Author(s): Panagiotis Agioutantis, Vassiliki Lila KoumandouAbstractThe human microbiome has lately emerged as an important factor in health and disease. The general patterns that emerge from metagenomics studies of the microbial diversity in the human gut of healthy adults, children and infants, are that (a) the microbiome is less diverse early in life, and gets enriched after infancy, and (b) that it is dominated by organisms from two bacterial phyla: bacteroidetes and firmicutes. However, there is still considerable diversity between individuals, based on age, habita...
Source: Meta Gene - July 11, 2018 Category: Genetics & Stem Cells Source Type: research