Association of MicroRNA related single nucleotide polymorphisms 196A-2 and 499 with the risk of hepatocellular carcinoma in Egyptian patients
ConclusionsMIR-SNP-499 might affect the susceptibility of Egyptians to HCC while, MIR-SNP-196A-2 may affect the HCC risk in Egyptians with HCV infection. (Source: Meta Gene)
Source: Meta Gene - July 10, 2018 Category: Genetics & Stem Cells Source Type: research

A simulations approach for meta-analysis of genetic association studies based on additive genetic model
Publication date: June 2018Source: Meta Gene, Volume 16Author(s): Majnu John, Todd Lencz, Anil K Malhotra, Christoph U Correll, Jian-Ping ZhangAbstractMeta-analysis of genetic association studies is being increasingly used to assess phenotypic differences between genotype groups. When the underlying genetic model is assumed to be dominant or recessive, assessing the phenotype differences based on summary statistics, reported for individual studies in a meta-analysis, is a valid strategy. However, when the genetic model is additive, a similar strategy based on summary statistics will lead to biased results. This fact about ...
Source: Meta Gene - July 10, 2018 Category: Genetics & Stem Cells Source Type: research

Association of interleukin-8 polymorphism (+781 C/T) with the risk of ovarian cancer
Publication date: June 2018Source: Meta Gene, Volume 16Author(s): Mohamad-Kian Zarafshani, Azin Shahmohammadi, Asad Vaisi-Raygani, Hoda Bashiri, Kheirollah YariAbstractIL-8 is a chemokine which plays an essential role in tumorigenesis, specifically in tumor growth, angiogenesis and invasion. Recent studies have determined that IL-8 +781C/T polymorphism correlates with some human cancers. The purpose of current study was to clarify the association of interleukin-8 polymorphism (+781 C/T) with the risk of ovarian cancer. In this case-control study, 150 samples including 50 specimens of ovarian tissue from female patients and...
Source: Meta Gene - July 10, 2018 Category: Genetics & Stem Cells Source Type: research

OncomiR-27a rs895819 variant and breast cancer risk: An updated meta-analysis
In conclusion overall miR-27a (rs895819) polymorphism was not associated with BC risk. However, the rs895819*G variant may confer protection against cancer in Caucasian and familial BC. Well-designed studies with larger cohorts in diverse ethnic populations are warranted. (Source: Meta Gene)
Source: Meta Gene - July 10, 2018 Category: Genetics & Stem Cells Source Type: research

Differential expression profiles of miRNAs and correlation with clinical outcomes in acute myeloid leukemia
ConclusionsDeregulated expressions of miRNAs pose significant impact on the clinical outcomes in AML patients. MicroRNA therapeutics indeed has a tremendous potential in the treatment of AML. (Source: Meta Gene)
Source: Meta Gene - July 10, 2018 Category: Genetics & Stem Cells Source Type: research

A potential association between mutations in the iNOS cDNA 3′ stretch and oral squamous cell carcinoma - A preliminary study
Publication date: June 2018Source: Meta Gene, Volume 16Author(s): Sapna Khowal, Swatantra Kumar Jain, Saima WajidAbstractOral cancers are linked with tobacco, areca nut and alcohol addictive proclivities. The oral squamous cell carcinoma (OSCC) comprises more than 90% cases of oral cancers. The Homo sapiens inducible nitric oxide synthase (iNOS) gene plays crucial role in OSCC pathologies. The given study analyzed the nucleotide mutations existent within two targets of interest namely iNOS cDNA 5′ stretch and iNOS cDNA 3′ stretch, using cDNA pool of cancer and pre-cancer oral biopsies employing Q PCR-Tm calling...
Source: Meta Gene - July 10, 2018 Category: Genetics & Stem Cells Source Type: research

A new mutation in steroidogenic acute regulatory protein (StAR) is segregated in an Iranian family
Publication date: June 2018Source: Meta Gene, Volume 16Author(s): Soudeh Ghafouri-Fard, Vahid Reza Yassaee, Nasrin Alipour, Zeinab Ravesh, Mohammad MiryounesiAbstractLipoid congenital adrenal hyperplasia (LCAH) is the most fatal form of defects in steroid hormone biosynthesis presented with in 46,XY sex reversal and severe fatal salt-losing crisis in the infancy. The disease is caused by mutation in the steroidogenic acute regulatory protein (StAR) coding gene. Several mutations have been found in this gene in different populations with the possible existence of a founder effect. In the current study, we present a novel mu...
Source: Meta Gene - July 10, 2018 Category: Genetics & Stem Cells Source Type: research

Association of genetic variants with macronutrient intake in Circassian and Chechan populations in relation to diabetes
Conclusionwe have identified a genetic association between intake of carbohydrate, calorie, vitamin B2 and caffeine in the Circassian population and calorie intake in the Chechan population. Three of these intake traits (carbohydrate, calorie and vitamin B2) were correlated with T2D development in Circassians. The association between macronutrient intake and diabetes development can shed light on causative variants for the pathogenesis of T2D. (Source: Meta Gene)
Source: Meta Gene - July 10, 2018 Category: Genetics & Stem Cells Source Type: research

Absence of APE1 (Asp148Glu) gene polymorphism in North-West Indian population: A comparison with world population
In this study, we evaluated genotype and allele frequencies of the APE1 (rs 1130409, Asp148Glu; T2197G) gene polymorphism in 450 subjects from North-West India and compared it with other Indian and world populations. Genomic DNA extraction was carried out using standard Phenol-chloroform method and ethanol precipitation. Genotyping was carried out by polymerase chain reaction- restriction fragment length polymorphism (PCR-RFLP) and run on 10% PAGE. Genotyping results were confirmed by direct DNA sequencing and running 10% of the samples in duplicate. It was found that APE1 (rs 1130409; Asp148Glu; T2197G) gene polymorphism ...
Source: Meta Gene - July 10, 2018 Category: Genetics & Stem Cells Source Type: research

Neuropeptide Y Leu7Pro polymorphism is not associated with risk of developing obesity in Pakistani population
ConclusionThe NPY SNP Leu7Pro is found to be extremely rare in Pakistani population and does not contribute towards risk of developing obesity. (Source: Meta Gene)
Source: Meta Gene - July 10, 2018 Category: Genetics & Stem Cells Source Type: research

Phylogeny based on 16S rRNA sequence and morphology of selected mosses of Mount Abu, Rajasthan (India)
We examined data set that comprises of ten moss species and four outgroup taxa drawn from liverworts. The molecular data contain nucleotide sequences derived from plastid DNA regions, i.e. partial 16S rRNA sequences and the morphological data included 18 characters derived from own observations and published literature. Congruence between the two data sets was investigated by computing several indices. The morphological data set contains poor phylogenetic signals. The results suggest that 1) the mosses, including Sphagnum and Takakia, are monophyletic, 2) Sphagnum and Takakia are placed together and sister to remaining mos...
Source: Meta Gene - July 10, 2018 Category: Genetics & Stem Cells Source Type: research

Association between interleukin-17 gene polymorphism and rheumatoid arthritis among Egyptians
ConclusionThe results of this study suggested that the IL-17A and IL-17F gene polymorphisms may not be associated with the susceptibility to RA among Egyptian population. (Source: Meta Gene)
Source: Meta Gene - July 10, 2018 Category: Genetics & Stem Cells Source Type: research

Association of Human Leucocyte Antigen (HLA) class II with systemic lupus erythematosis (SLE) patients from western India
ConclusionsOur data suggest an association between HLA class II with susceptibility to SLE in the Indian population. DQB1*05 and DQA1*01 are strongly positively associated with SLE in western Indian SLE patients. DRB1*04, DRB1*11, DQB1*03 and DQA1*03 are negatively associated with SLE in western India. (Source: Meta Gene)
Source: Meta Gene - July 10, 2018 Category: Genetics & Stem Cells Source Type: research

Association of APOE − 219G > T and −427T > C polymorphic variants in transcriptional regulatory region of APOE gene with gallbladder stone: A north Indian case-control study
Publication date: June 2018Source: Meta Gene, Volume 16Author(s): Shipra Dwivedi, Amit Kumar Madeshiya, Shraddha Singh, Abhjeet Chandra, Abbas Ali MahdiAbstractGallbladder stone (GBS) is a major cause of morbidity and mortality throughout the world including India. The significant role of APO E in the regulation of lipid metabolism raises the possibility that APO E promoter gene polymorphisms may be a predisposing factor for GBS formation. In this case control study total 358 subjects (n = 102 GBS subjects and n = 256 healthy control) between the age group of 18 to 70 years were enrolled. Three APO E promoter gen...
Source: Meta Gene - July 10, 2018 Category: Genetics & Stem Cells Source Type: research

Report of three cases with hereditary spastic paraplegia and investigation of the mutations
Publication date: June 2018Source: Meta Gene, Volume 16Author(s): Mohammad Miryounesi, Seyed Mohammad Bagher Tabei, Mehdi Dianatpour, Majid Fardaei, Soudeh Ghafouri-FardAbstractHereditary spastic paraplegia (HSP) includes a group of clinically and genetically heterogeneous disorders with multiple genetic loci participating in its pathogenesis. Here we report three Iranian patients with HSP. Molecular analyses revealed that they have mutations in AP4M1 gene (NM_004722:c.802C>T, p.R268X and c.1225T>C, p.F409L) and AP4E1 gene (NM_007347: c.3212_3213delCT, p.L1072Afs*10). All of them were born to consanguineous parents. ...
Source: Meta Gene - July 10, 2018 Category: Genetics & Stem Cells Source Type: research

Absence of association between TLR4 Thr399Ile polymorphism and cervical cancer susceptibility
ConclusionComplete absence of T allele in our study subjects demonstrate no involvement of TLR4 Thr399Ile polymorphism in cervical cancer susceptibility. However, an association between increased age and late clinical staging was observed. A comprehensive analysis on a larger sample size covering diverse ethnic populations of India is warranted to comprehend the role of TLR4 Thr399Ile polymorphism. (Source: Meta Gene)
Source: Meta Gene - July 10, 2018 Category: Genetics & Stem Cells Source Type: research

Molecular phylogeny unveils hidden diversity of hillstream loaches (Cypriniformes: Cobitoidea) in the northern Western Ghats of India
Publication date: Available online 9 July 2018Source: Meta GeneAuthor(s): Ashwini Keskar, Rajeev Raghavan, Mandar S. Paingankar, Pradeep Kumkar, Unmesh Katwate, Shrikant Jadhav, Anand Padhye, Neelesh DahanukarAbstractThe diversity of hillstream loaches in the northern part of the Western Ghats region of India was studied for the first time using two mitochondrial genes, cytochrome oxidase subunit I and cytochrome b. Phylogenetic analysis revealed monophyletic groups corresponding to the genera Indoreonectes, Paracanthocobitis, Nemacheilus, Schistura and Nemachilichthys (family Nemacheilidae), and Balitora, Lepidocephalicht...
Source: Meta Gene - July 10, 2018 Category: Genetics & Stem Cells Source Type: research

Comparing polymorphism of 86 candidate genes putatively involved in domestication of sheep, between wild and domestic Iranian sheep
In this study, we compared genetic diversity of 13 Iranian Mouflons (Ovis orientalis) and 20 Iranian domestic sheep (Ovis aries) based on 86 candidate genes putatively involved in the domestication of sheep. Mean nucleotide diversity and mean expected heterozygosity of candidate genes calculated by means of VCF tools and statistical analysis were performed via IBM SPSS software. Our results showed that Mouflon was superior for both calculated diversity parameters in the majority of candidate genes under study. In fact, wild group showed higher mean nucleotide diversity and mean heterozygosity in 69 (32 statistically signif...
Source: Meta Gene - July 10, 2018 Category: Genetics & Stem Cells Source Type: research

Circulating miR-196a-5p miR-373-3p and miR-375: Novel candidate biomarkers for diagnosis of acute coronary syndrome
In this study, total 135 patients with angina or acute myocardial infarction who underwent coronary angiography were recruited and divided into 3 groups: 45 patients with normal coronary arteries, 45 patients with obstructive CAD and 45 patients with acute STEMI. Serum which was obtained from blood samples were drawn before coronary angiography. After RNA isolation and cDNA synthesis, expressions of miRNAs were detected by quantitative real-time polymerase chain reaction (qRT-PCR). Statistical analysis of qRT-PCR expression results was carried out by using the 2−ΔCt formula.ResultsExpression differences were st...
Source: Meta Gene - July 10, 2018 Category: Genetics & Stem Cells Source Type: research

Association of BRCA2 variants with breast cancer risk: A meta-analysis
Publication date: September 2018Source: Meta Gene, Volume 17Author(s): Sepideh Faramarzi, Ali Dianatpour, Soudeh Ghafouri-FardAbstractBRCA2 is a tumor suppressor gene involved in repair of double strand breaks in DNA. Germ line mutations within this gene are implicated in familial breast and ovary cancer syndromes. Moreover, several studies have assessed association between single nucleotide polymorphisms (SNPs) within this gene and risk of breast cancer. We performed a meta-analysis to assess the association between rs4987117, rs1799955, rs766173, rs15869 SNPs within BRCA2 gene and risk of breast cancer. The pooled odds r...
Source: Meta Gene - July 10, 2018 Category: Genetics & Stem Cells Source Type: research

Association study of six single nucleotide polymorphisms with obesity in two independent Iberian samples
In this study, we investigate the reproducibility in two independent samples of the association and weighted GRS previously proposed for six SNPs as predictors for obesity susceptibility. A case-control study was performed using one sample comprising 346 unrelated Portuguese children [6–12 years-old; 191 controls and 155 obese], and a second sample comprising 332 unrelated Spanish adults [18–60 years-old; 217 controls and 115 obese]. Polymorphisms located on FTO, TFAP2B, SEC16B, ETV5, and SH2B1 genes were genotyped by allelic discrimination TaqMan assays.After Bonferroni corrections we found nominal signifi...
Source: Meta Gene - July 10, 2018 Category: Genetics & Stem Cells Source Type: research

Mutation screening of the BRCA1 gene in sporadic breast cancer in the Central of Iran
Publication date: September 2018Source: Meta Gene, Volume 17Author(s): Mandana Behbahani, Mokhtar Nosrati, Hassan MohabatkarAbstractThe purpose of the work was to study mutation screening of BRCA1 in Iranian patients with sporadic breast cancer. We carried out a mutational analysis of BRCA1 gene in 101 breast cancer patients from a population in Central of Iran. The comparison of DNA of paraffin-embedded breast cancer tissue from patients was studied, and breast tissue from 30 unrelated normal women without cancer was selected as controls. The entire BRCA1 coding sequence was amplified by PCR with primers especially design...
Source: Meta Gene - July 10, 2018 Category: Genetics & Stem Cells Source Type: research

Detect the presence of LeIF gene in the Leishmania tropica genome and sequence it
Publication date: September 2018Source: Meta Gene, Volume 17Author(s): Razan Abbara, Mohammad MaaroufAbstractLeishmaniasis remains one of the most neglected diseases globally and affects primarily the poor in developing countries. Due to the absence of safe and effective drug, the prevention of infection of this disease is very important as it requires an effective vaccine is not yet available. Leishmania eukaryotic initiation factor (LeIF) protein acts an inhibitor of the Leishmania major growth in murine macrophages and reduces interleukin-4 in lymph nodes. We aimed to detect its presence in Leishmania tropica genome and...
Source: Meta Gene - July 10, 2018 Category: Genetics & Stem Cells Source Type: research

Subtractive proteomics revealed plausible drug candidates in the proteome of multi-drug resistant Corynebacterium diphtheriae
Publication date: September 2018Source: Meta Gene, Volume 17Author(s): Zunera Khalid, Sajjad Ahmad, Saad Raza, Syed Sikander AzamAbstractMulti-drug resistant Corynebacterium diphtheriae, an etiological agent of diphtheria, is a major health concern because of its resistance to major clinically used antibiotics. The said pathogen is highly resistant to lincosamides, macrolides, streptogramin, tetracycline, trimethoprim, chloramphenicol and benzylpenicillin and as such, required an urgent need of novel drug targets identification. Here, in this present study, we employed an in silico based approach for identifying potential ...
Source: Meta Gene - July 10, 2018 Category: Genetics & Stem Cells Source Type: research

The investigation of miR-196a2 rs11614913 with breast cancer susceptibility in south of IRAN
ConclusionsThis study's results indicated that the TT polymorphic genotype in hsa-miR-196a2 rs11614913 SNP was not related to breast cancer risk among Southern Iranian patients. (Source: Meta Gene)
Source: Meta Gene - July 10, 2018 Category: Genetics & Stem Cells Source Type: research

Mutations in TYR and OCA2 associated with oculocutaneous albinism in Pakistani families
ConclusionsOur findings broaden the molecular spectrum associated with TYR and OCA2 mutations in Pakistani families, aiding the development and refinement of genetic diagnostic and counselling services in Pakistan. (Source: Meta Gene)
Source: Meta Gene - July 10, 2018 Category: Genetics & Stem Cells Source Type: research

ATF6 polymorphisms and protective effect in diabetic retinopathy
Publication date: September 2018Source: Meta Gene, Volume 17Author(s): Mina Tayyab, Sundus Ijaz Maqsood, Aisha Azam, Irfan Muslim, Zehra Agha, Sobia Shafique, Waqas Ahmed, Muhammad Khizar Niazi, Mazhar Ishaq, Nadia Khalida Waheed, Maleeha Azam, Raheel QamarAbstractActivating transcription factor 6 (ATF6) has been observed to be a strong contributor in pathology of diabetes via its angiogenesis and apoptosis role, therefore in the present study we investigated the role of ATF6 polymorphisms (rs1058405; rs11579627; rs13401) in diabetic retinopathy (DR) susceptibility in Pakistani type 2 diabetes mellitus (T2DM) persons. ATF6...
Source: Meta Gene - July 10, 2018 Category: Genetics & Stem Cells Source Type: research

Discovering microRNAs and their targets in the red flour beetle Tribolium castaneum from expressed sequence tags
Publication date: September 2018Source: Meta Gene, Volume 17Author(s): Chandan Badapanda, Ankita RathoreAbstractMicroRNAs (miRNAs) are endogenous, single stranded, small RNAs that are 18–24 nucleotides in length, ubiquitously found in eukaryotes and in some viruses. miRNAs majorly help in down regulation of genes by binding to the complimentary sites on 3′ untranslated regions of target messenger RNA (mRNAs), and more than 30% of the genes in animals are believed to be under the control of miRNAs. The red flour beetle, Tribolium castaneum is a global pest of stored agricultural products. The use of insecticides...
Source: Meta Gene - July 10, 2018 Category: Genetics & Stem Cells Source Type: research

Shortening the list of essential genes in the human genome by network analysis
Publication date: September 2018Source: Meta Gene, Volume 17Author(s): Rasoul Godini, Hossein FallahiAbstractRecently, several lists for human essential genes have been presented by several studies. Expectedly, many known and important genes are among those that are crucial for cellular activities. However, in-depth analysis of these genes is required to fully understand their functions and involvements in cellular functions. Besides, finding the regulatory elements responsible for essential genes expression would be helpful to understand the core gene regulatory network of the cell. Although the primary reports on essenti...
Source: Meta Gene - July 10, 2018 Category: Genetics & Stem Cells Source Type: research

Evaluation of caries experience in two genders and ENAM polymorphism in Iranian adults
In this study, the caries rates for females were approximately two times higher than the frequency of caries among males. The genotyping results showed that there were no differences in allelic and genotypic frequencies between the case and control groups. Further studies are required to identify the susceptible loci for dental caries in our case and control groups. (Source: Meta Gene)
Source: Meta Gene - July 10, 2018 Category: Genetics & Stem Cells Source Type: research

Expression analysis of CBR3-AS1 and androgen receptor genes in breast cancer
Publication date: September 2018Source: Meta Gene, Volume 17Author(s): Sepideh Faramarzi, Ali Dianatpour, Lobat Geranpayeh, Reza Mirfakhraie, Roshanak Shams, Mahnaz Seifi-Alan, Vahid Kholghi Oskooei, Soudeh Ghafouri-FardAbstractLong non-coding RNAs (lncRNAs) have been shown to participate in the pathogenesis of a variety of cancers including breast cancer. The lncRNA Carbonyl reductase 3-Antisense 1 (CBR3-AS1) has been implicated in some human malignancies in association with androgen receptor (AR). In the present study, we evaluated the expression of CBR3-AS1 and AR transcripts in 52 breast cancer tissues, their correspon...
Source: Meta Gene - July 10, 2018 Category: Genetics & Stem Cells Source Type: research

Association of polymorphism in P16 and myeloperoxidase genes with susceptibility to oral lesions in North Indian population
Publication date: September 2018Source: Meta Gene, Volume 17Author(s): Parul Tripathi, Aditi Singh, Somali Sanyal, Suresh Kumar Yadav, Kumud NigamAbstractBackgroundExpression of P16 and MPO (myeloperoxidase) has been proposed as a marker for malignant transformation. In MPO, a G/A polymorphism at 463 bp upstream of the transcription start site of the enzyme has functional outcome, where G allele results in higher level of MPO expression than A allele. Protein P16, a key regulator in cell cycle, is also commonly associated with cancer. SNP of P16 exon3 (C540G) has been found to be linked with tumor aggressiveness.Objectiv...
Source: Meta Gene - July 10, 2018 Category: Genetics & Stem Cells Source Type: research

Analysis of ABL kinase domain mutations as a probable cause of imatinib resistance in Chronic Myeloid Leukemia patients of Kashmir
Publication date: September 2018Source: Meta Gene, Volume 17Author(s): Niyaz A. Azad, Zafar A. Shah, Arshad A. Pandith, Roohi Rasool, Javed A. Rasool, Shahid M. Baba, Sheikh A. Aziz, Samoon JeelaniAbstractThe phenomenon of imatinib resistance in Chronic Myeloid Leukemia (CML) can be mediated by “BCR-ABL fusion transcript dependent” and “BCR-ABL fusion transcript independent” pathways. The main means of “BCR-ABL fusion transcript dependent” pathway, responsible for 40–90% of such cases, is the occurrence of Kinase Domain (KD) mutations. The aim of this study was to evaluate the comm...
Source: Meta Gene - July 10, 2018 Category: Genetics & Stem Cells Source Type: research

Spectrum of clinical manifestations of SLE patients from India and its correlation with KIR gene polymorphism
ConclusionQuite a few of the genes of the KIR, which are not uncommon in Indian population are also significantly associated with SLE in Indian patients. Few KIR genes can be used as biomarkers to understand the severity of the disease. The KIR genes in this cohort lead to decreased inhibitory signal and increased activation of NK cells and therefore causing increased apoptosis and formation of autoantibodies. (Source: Meta Gene)
Source: Meta Gene - July 10, 2018 Category: Genetics & Stem Cells Source Type: research

Influence of TP53 gene somatic mutations in Helicobacter pylori infected gastric tumor
Publication date: September 2018Source: Meta Gene, Volume 17Author(s): Souvik Ghatak, Payel Chakraborty, Subbarayan Sarathbabu, Jeremy L. Pautu, John Zohmingthanga, C. Lalchhandama, Nachimuthu Senthil KumarAbstractGenetic alteration in TP53 gene can proliferate the tumor formation in gastric cancer and the frequency of these somatic mutations can vary in different geographical populations. In the present study, sequencing of the coding regions of TP53 gene, real time gene expression and immunohistochemistry analysis were performed in gastric tumor samples. H. pylori infected tumor, with or without cagA genotype revealed hi...
Source: Meta Gene - July 10, 2018 Category: Genetics & Stem Cells Source Type: research

Association of glutathione peroxidase 1 gene polymorphism (rs1050450) with Hashimoto's thyroiditis in Northwest Iran
Publication date: September 2018Source: Meta Gene, Volume 17Author(s): Zafar Gholinejad, Amir Hossein Faghfouri, Rasoul Zarrin, Yousef RasmiAbstractHashimoto's thyroiditis (HT) is an inflammatory and autoimmune thyroid disorder. The oxidative stress and inflammation crosstalk play pivotal role in the pathogenesis of HT. The activity of glutathione peroxidase-1 (GPx-1) enzyme is affected by single nucleotide polymorphism in the rs1050450 position, which influence the risk of several diseases. The aim of this study is to determine the association of rs1050450 genotype variant and the HT risk. A hundred and five patients HT p...
Source: Meta Gene - July 10, 2018 Category: Genetics & Stem Cells Source Type: research

Role of osteopontin and its rs11730582 gene polymorphism in breast cancer
Publication date: September 2018Source: Meta Gene, Volume 17Author(s): Madeha M. Zakhary, Aida A. Mahmoud, Marwa S. HashimAbstractBackgroundOsteopontin (OPN) is an extracellular matrix protein of the integrin family that is involved in several biological pathways. Genetic polymorphisms in OPN gene has been found associated with many types of cancer.AimsIn this investigation, we aimed to study the possible role of OPN and its rs11730582 C/T single nucleotide polymorphism (SNP) in patients with breast cancer.MethodsOPN plasma levels were measured by ELIZA and OPN rs11730582 was genotyped by Taqman genotyping assay in 60 brea...
Source: Meta Gene - July 10, 2018 Category: Genetics & Stem Cells Source Type: research

Mannose binding lectin-2 gene functional polymorphisms in chronic periodontitis patients; a report from Iran
Publication date: September 2018Source: Meta Gene, Volume 17Author(s): Somayeh Ansari Moghadam, Ali Bazi, Masoud Miri-Moghaddam, Ebrahim Miri-MoghaddamAbstractChronic Periodontitis (CP)1 is a bacterial inflammatory condition accompanied with destruction of tissues surrounding periodontal ligament and tooth loss. There are reports of associations between mannose binding lectin-2 (MBL2)2 gene polymorphisms and susceptibility to bacterial infections, however, a few studies have been performed on the role of MBL polymorphisms in periodontal diseases. Here, we assessed the potential impacts of two functional polymorphisms in MB...
Source: Meta Gene - July 10, 2018 Category: Genetics & Stem Cells Source Type: research

Molecular analysis of drug-resistant Acinetobacter baumannii isolates by ERIC-PCR
Publication date: September 2018Source: Meta Gene, Volume 17Author(s): Hamid Heidari, Mehrdad Halaji, Asieh Taji, Hossein Kazemian, Milad Shahini Shams Abadi, Marzie Taheripour Sisakht, Hadi Sedigh Ebrahim-SaraieAbstractAcinetobacter baumannii is an important pathogen that associated with several hospital-acquired infections. It has the ability to colonize in hospital setting and infections due to A. baumannii can be transferred by direct or indirect contact between patients. Characterization of the local molecular epidemiology is required for control the spread of these strains. Therefore, this study was designed to inves...
Source: Meta Gene - July 10, 2018 Category: Genetics & Stem Cells Source Type: research

In silico assessment of human CD14 gene revealed high-risk single nucleotide polymorphisms and their impact on innate immune response against microbial pathogens
In conclusion, our data suggests that disruption of CD14 structure by SNPs rs767220208, rs745518361, rs754968319, rs764993455 and rs749260147 could affect activation of the innate immunity. (Source: Meta Gene)
Source: Meta Gene - July 10, 2018 Category: Genetics & Stem Cells Source Type: research

4-bp insertion/deletion polymorphism within the promoter of EGLN2 gene is associated with susceptibility to cancer in Asian population: Evidence from a meta-analysis
Publication date: September 2018Source: Meta Gene, Volume 17Author(s): Mohammad Hashemi, Farhad Tabasi, Hossein AnsariAbstractWe conducted the current meta-analysis to quantitatively summarize the evidence for the strength of the associations between 4-bp insertion/deletion (I/D) polymorphism (rs10680577) of EGLN2 and cancer risk. Using databases searches, we included 5 case-control studies encompassing 3030 cases and 4728 controls. The pooled odds ratios (ORs) and 95% confidence intervals (CIs) were calculated. The findings of this meta-analysis highlighted that the 4-bp I/D polymorphism of EGLN2 significantly increased t...
Source: Meta Gene - July 10, 2018 Category: Genetics & Stem Cells Source Type: research

An alternative in vitro plant regeneration system in papaya (Carica papaya L.) through callus derived nodular cultures
Publication date: September 2018Source: Meta Gene, Volume 17Author(s): R. Anandan, T. Deenathayalan, Nukala Sumanth Kumar, K.V. DeepakAbstractIn vitro regeneration systems either based on organogenesis or somatic embryogenesis has been well established in Carica papaya L. Whereas a third morphogenetic pathway involving plant regeneration system through nodular cultures have not been reported. Here, we report an efficient and rapid in vitro regeneration system through callus derived nodular culture in papaya cultivar CO7. Excised zygotic embryos were cultured onto Murashige and Skoog (MS) medium supplemented with different ...
Source: Meta Gene - July 10, 2018 Category: Genetics & Stem Cells Source Type: research

α-Adducin nsSNPs affect mRNA secondary structure, protein modification and stability
In this study, thirty six nsSNPs were subjected to in silico analyses using different computational tools. It is observed that out of thirty six SNP sites of ADD1, position of sixteen residues is highly conserved. It has been found that four nsSNPs have a very low tolerance index (TI) of 0.00 and four nsSNPs show a TI score of 0.01. Fifteen nsSNPs which are predicted as deleterious by the SIFT Server, are also found to be probably damaging by Polyphen Server. Highest pathogenicity score is predicted for Y270N variant of ADD1, followed by R231C, R354C, R396Q, K148I, E376D, L387M, and L387V variants. Several nsSNPs of ADD1 a...
Source: Meta Gene - July 10, 2018 Category: Genetics & Stem Cells Source Type: research

Association of cholesteryl ester transferyl protein gene (G277A) polymorphism and risk prediction of type 2 diabetes mellitus: A case control study of Northern Indian population
ConclusionIn our study CETP (G277A) gene polymorphism is not associated with T2DM but significantly affect various lipid traits and PON1 levels. (Source: Meta Gene)
Source: Meta Gene - July 10, 2018 Category: Genetics & Stem Cells Source Type: research

Identification of a novel ALMS1 pathogenic variant in a family with Cone-Rod Dystrophy using whole exome sequencing, followed by prenatal diagnosis
ConclusionsThis study provides further evidence that how recently developed high throughput sequencing can overcome the diagnostic hurdles encountered in heterogeneous disorders. It also demonstrates that this approach provides clinicians with new tools for early diagnosis, management, and prevention of such otherwise clinically hard-to-detect disorders. Hence, the “genotype-first” approach presented here is more likely to uncover the diagnosis of heterogeneous disorders compared to traditionally “phenotype-first” attitude. (Source: Meta Gene)
Source: Meta Gene - July 10, 2018 Category: Genetics & Stem Cells Source Type: research

Association of an intronic SNP of the EFEMP1 gene with height in Tongans
The objective of this study is to examine the possible association of rs3791675-G with taller height in Oceanian populations. The rs3791675 SNP was genotyped for 636 adult subjects living in Tonga (Tonga population) and Solomon Islands (Munda, Kusaghe, and Rawaki populations). The allele frequency of rs3791675-G ranged from 0.29 to 0.66. A multiple regression analysis adjusted for age and sex was performed to test the association of rs3791675 with height in each population. The results revealed that a single copy of the rs3791675-G allele significantly increased height by 1.2 cm in Tonga population (P-value = 0.031),...
Source: Meta Gene - July 10, 2018 Category: Genetics & Stem Cells Source Type: research

Genetic aspects of sexual size dimorphism in a synthesized breed of sheep
Publication date: September 2018Source: Meta Gene, Volume 17Author(s): Farhad Ghafouri-Kesbi, Hasan BanehAbstractThe aim was to study genetic aspects of sexual size dimorphism in Iran-Black synthetized breed of sheep. Traits evaluated were birth weights (BW), weaning weights (WW) and post-weaning body weights measured at 6 months (W6), 9 months (W9) and 12 months (W12) of age. A series of six bivariate animal models including direct and maternal components were used to measure phenotypic variance and its constituent components in male and female lambs. Male lambs were heavier than female lambs by 6%, 10%, 9%, 10% and...
Source: Meta Gene - July 10, 2018 Category: Genetics & Stem Cells Source Type: research

High throughput sequence profiling of gut microbiome in Northern Indian infants during the first four months and its global comparison
Publication date: September 2018Source: Meta Gene, Volume 17Author(s): Sampan Attri, Ravinder Nagpal, Gunjan GoelAbstractThe present study characterized the colonization and development of gut microbial communities in healthy Indian infants from North-Western Himalayan region in the province Himachal Pradesh. The diversity and transitions of core genera was assessed targeting the 16S rRNA V3-V4 hypervariable region on an Illumina platform. Analysis of more than 17,000 filtered high quality reads indicated that the diversity was lowest in the month 2 followed by gradual increase towards month 4 (1.24 folds increase in Shann...
Source: Meta Gene - July 10, 2018 Category: Genetics & Stem Cells Source Type: research

Circulating tumor DNA, liquid biopsy, and next generation sequencing: A comprehensive technical and clinical applications review
Publication date: September 2018Source: Meta Gene, Volume 17Author(s): Sarah Abou Daya, Rami MahfouzAbstractCirculating tumor DNA (ctDNA) represents a small fraction of the total circulating free DNA and its analysis is increasingly used for diagnostic, prognostic and treatment purposes of cancer.ctDNA is released into the bloodstream from tumor cells through different mechanisms including apoptosis, necrosis, autophagy, and necroptosis. Liquid biopsy is a method used to detect specific cancer mutations in ctDNA from the plasma fraction of a standard blood draw and has numerous applications. Adoption of this newly introduc...
Source: Meta Gene - July 10, 2018 Category: Genetics & Stem Cells Source Type: research

Association of INS VNTR polymorphisms with polycystic ovary syndrome (PCOS) in south Indian population
Publication date: September 2018Source: Meta Gene, Volume 17Author(s): Shiwangi Dwivedi, Raghav Sharma, Prasanna Kumar Shetty, Anirban Chakraborty (Source: Meta Gene)
Source: Meta Gene - July 10, 2018 Category: Genetics & Stem Cells Source Type: research