Long-label-retaining mammary epithelial cells are created early in ductal development and distributed throughout the branching ducts
Publication date: Available online 20 July 2019Source: Mechanisms of DevelopmentAuthor(s): Lauren N. Ragle, Robert D. Bruno, Corinne A. Boulanger, Gilbert H. SmithAbstractLong-label retention has been used by many to prove Cairns' immortal strand hypothesis and to identify potential stem cells. Here, we describe two strategies using 5-ethynl-2′-deoxyuridine (EdU) to identify and understand the distribution of long-label-retaining mammary epithelial cells during formation of the mouse mammary ductal system. First, EdU was given upon two consecutive days per week during weeks 4 through 10 and analyzed for label retention a...
Source: Mechanisms of Development - July 20, 2019 Category: Biology Source Type: research
Editorial Board
Publication date: August 2019Source: Mechanisms of Development, Volume 158Author(s): (Source: Mechanisms of Development)
Source: Mechanisms of Development - July 13, 2019 Category: Biology Source Type: research
Use of in vitro electroporation and slice culture for gene function analysis in the mouse embryonic spinal cord
In this study, we have demonstrated the expression of an exogenous gene on one side of the spinal cord by combining two methods—in vitro electroporation of embryonic mouse spinal cord and organ spinal cord slices culture. We took 12-day embryonic mice, injected the green fluorescent protein (pCAGGS-GFP) plasmid into the spinal cord cavity in vitro, and then electroporated. The spinal cord was cut into 300-μm slices using a vibratory microtome. After cultured for 48 h, GFP-positive neurons were clearly observed on one side of the spinal cord, indicating that the exogenous gene was successfully transferred. The axon pro...
Source: Mechanisms of Development - June 16, 2019 Category: Biology Source Type: research
Nicole Le Douarin and the use of quail-chick chimeras to study the developmental fate of neural crest and hematopoietic cells
This article summarizes the fundamental contribution of Nicole Le Douarin to the discovery and the application of this technique over the last 40 years. (Source: Mechanisms of Development)
Source: Mechanisms of Development - May 24, 2019 Category: Biology Source Type: research
Single origin of the epithelium of the human middle ear
ConclusionDifferent morphological epithelial appearances throughout the tympanic and temporal bone cavities could be explained by different stages of epithelial differentiation rather than different embryologic origin and endodermal rupture does not seem to be a necessity for these cavities to form. (Source: Mechanisms of Development)
Source: Mechanisms of Development - May 21, 2019 Category: Biology Source Type: research
The microRNA-306/abrupt regulatory axis controls wing and haltere growth in Drosophila
Publication date: Available online 18 May 2019Source: Mechanisms of DevelopmentAuthor(s): Carolina J. Simoes da Silva, Ismael Sospedra, Ricardo Aparicio, Ana BusturiaAbstractGrowth control relies on extrinsic and intrinsic mechanisms that regulate and coordinate the size and pattern of organisms. This control is crucial for a homeostatic development and healthy physiology. The gene networks acting in this process are large and complex: factors involved in growth control are also important in diverse biological processes and these networks include multiple regulators that interact and respond to intra- and extra-cellular in...
Source: Mechanisms of Development - May 19, 2019 Category: Biology Source Type: research
Editorial Board
Publication date: June 2019Source: Mechanisms of Development, Volume 157Author(s): (Source: Mechanisms of Development)
Source: Mechanisms of Development - May 18, 2019 Category: Biology Source Type: research
The transcription factor Foxp1 regulates the differentiation and function of dendritic cells
In this study, we found that the expression of the transcription factor Foxp1 gradually increased during the maturation of DCs. Then, we constructed a recombinant adenovirus carrying Foxp1-interfering RNA (Ad-simFoxp1) and transfected murine bone marrow-derived DCs in vitro. DCs transfected with Ad-simFoxp1 exhibited markedly lower costimulatory molecules, and decreased cytokines. And Ad-simFoxp1 greatly inhibited mature DC-induced T cell responses. Moreover, in vivo infusion with Ad-simFoxp1-modified DCs significantly delayed the onset of experimental autoimmune encephalomyelitis (EAE). Therefore, adoptive transfection of...
Source: Mechanisms of Development - May 8, 2019 Category: Biology Source Type: research
High temperature limits on developmental canalization in the ascidian Ciona intestinalis
This study reveals a high temperature threshold, occurring over a 1–2 °C range, for normal embryonic development in C. intestinalis. Above that threshold the prevalence of morphological abnormalities increases significantly, beginning with cleavage and gastrula stages, and becoming more pronounced as embryogenesis proceeds. However, even in highly morphologically abnormal temperature disrupted (TD) embryos, muscle, endoderm, notochord, epidermis, and sensory pigment cells are recognizable, as evidenced by histochemical markers or morphology. On the other hand, morphogenesis of the notochord and other structures is dep...
Source: Mechanisms of Development - April 25, 2019 Category: Biology Source Type: research
Editorial Board
Publication date: April 2019Source: Mechanisms of Development, Volume 156Author(s): (Source: Mechanisms of Development)
Source: Mechanisms of Development - April 25, 2019 Category: Biology Source Type: research
Synchronization of Hes1 oscillations coordinates and refines condensation formation and patterning of the avian limb skeleton
Publication date: April 2019Source: Mechanisms of Development, Volume 156Author(s): Ramray Bhat, Tilmann Glimm, Marta Linde-Medina, Cheng Cui, Stuart A. NewmanAbstractThe tetrapod appendicular skeleton is initiated as spatially patterned mesenchymal condensations. The size and spacing of these condensations in avian limb buds are mediated by a reaction-diffusion-adhesion network consisting of galectins Gal-1A, Gal-8 and their cell surface receptors. In cell cultures, the appearance of condensations is synchronized across distances greater than the characteristic wavelength of their spatial pattern. We explored the possible...
Source: Mechanisms of Development - April 16, 2019 Category: Biology Source Type: research
Zebrafish otolith biomineralization requires polyketide synthase
This study will serve as a basis for investigating the role of eNOS signaling and Endothelin-1 signaling during otolith formation. (Source: Mechanisms of Development)
Source: Mechanisms of Development - April 8, 2019 Category: Biology Source Type: research
Prenatal catch-up growth: A study in avian embryos
In conclusion, in this experimental model of CUGr, the embryo's weight at the end of a stunt could fully predict and explain the rate of growth during the post-intervention recovery period. (Source: Mechanisms of Development)
Source: Mechanisms of Development - March 30, 2019 Category: Biology Source Type: research
Developmental regulation of Wnt signaling by Nagk and the UDP-GlcNAc salvage pathway
Publication date: Available online 20 March 2019Source: Mechanisms of DevelopmentAuthor(s): Leif R. Neitzel, Zachary T. Spencer, Anmada Nayak, Christopher S. Cselenyi, Hassina Benchabane, CheyAnne Q. Youngblood, Alya Zouaoui, Victoria Ng, Leah Stephens, Trevor Hann, James G. Patton, David Robbins, Yashi Ahmed, Ethan LeeAbstractIn a screen for human kinases that regulate Xenopus laevis embryogenesis, we identified Nagk and other components of the UDP-GlcNAc glycosylation salvage pathway as regulators of anteroposterior patterning and Wnt signaling. We find that the salvage pathway does not affect other major embryonic signa...
Source: Mechanisms of Development - March 21, 2019 Category: Biology Source Type: research
Synchronization of Hes1 oscillations coordinate and refine condensation formation and patterning of the avian limb skeleton
Publication date: Available online 10 March 2019Source: Mechanisms of DevelopmentAuthor(s): Ramray Bhat, Tilmann Glimm, Marta Linde-Medina, Cheng Cui, Stuart A. NewmanAbstractThe tetrapod appendicular skeleton is initiated as spatially patterned mesenchymal condensations. The size and spacing of these condensations in avian limb buds are mediated by a reaction-diffusion-adhesion network consisting of galectins Gal-1A, Gal-8 and their cell surface receptors. In cell cultures, the appearance of condensations is synchronized across distances greater than the characteristic wavelength of their spatial pattern. We explored the ...
Source: Mechanisms of Development - March 11, 2019 Category: Biology Source Type: research
