Rapid and simple method by combining FTA ™ card DNA extraction with two set multiplex PCR for simultaneous detection of non‐O157 Shiga toxin‐producing Escherichia coli strains and virulence genes in food samples
This study also determined the successful ability of the FTA™ card as an alternative to commercial DNA extraction method for conducting multiplex STEC PCR assays. The FTA™ card combined with multiplex PCR holds promise for the food industry by offering a simple and rapid DNA sample method for reducing time, cost and labour for detection of STEC in food and environmental samples.
Significance and Impact of the Study: Two multiplex polymerase chain reaction (PCR) assays were optimized for discrimination of six non‐O157 Shiga toxin‐producing Escherichia coli (STEC) and identification of their major virulence genes wi...
Source: Letters in Applied Microbiology - October 24, 2017 Category: Microbiology Authors: S.A. Kim, S.H. Park, S.I. Lee, S.C. Ricke Tags: Original Article Source Type: research
A vapour phase assay for evaluating the antimicrobial activities of essential oils against bovine respiratory bacterial pathogens
In this study, we present a new vapour phase assay for evaluating the antimicrobial activities of essential oils (EO) against bovine respiratory pathogens (BRPs). Using this assay, we identified EOs, such as ajowan, thyme and cinnamon leaf, that can effectively inhibit growth of the BRPs Mannheimia haemolytica serotype 1, Pasteurella multocida and Histophilus somni. This is the first study to demonstrate the vapour phase antimicrobial activity of EOs against BRPs.
Significance and Impact of the Study: In this study, we present a new vapour phase assay for evaluating the antimicrobial activities of essential oils (EO) agai...
Source: Letters in Applied Microbiology - October 24, 2017 Category: Microbiology Authors: S. Amat, D. Baines, T.W. Alexander Tags: Original Article Source Type: research
Central carbon metabolism influences cellulase production in Bacillus licheniformis
This article is protected by copyright. All rights reserved. (Source: Letters in Applied Microbiology)
Source: Letters in Applied Microbiology - October 24, 2017 Category: Microbiology Authors: Jihui Wang, Shiwen Liu, Ying Li, Han Wang, Shan Xiao, Cheng Li, Bingnan Liu Tags: Original Article Source Type: research
Isolation and Characterization of multi ‐drug resistant Leclercia species from Animal clinical case
This article is protected by copyright. All rights reserved. (Source: Letters in Applied Microbiology)
Source: Letters in Applied Microbiology - October 24, 2017 Category: Microbiology Authors: Mamta Choudhary, Binod Kumar Choudhary, Smita Bhoyar, Satyajit B. Kale, S.P. Chaudhari, B.C. Bera, Ashok Jain, S. B. Barbuddhe Tags: Original Article Source Type: research
Bacteriocin ‐like inhibitory activities of seven Lactobacillus delbrueckii subsp. bulgaricus strains against antibiotic susceptible and resistant Helicobacter pylori strains
In this study, anti‐Helicobacter pylori activity of seven Lactobacillus delbrueckii subsp. bulgaricus (GLB) strains was evaluated by four cell‐free supernatant (CFS) types. The GLB strains produced heat‐stable bacteriocin‐like inhibitory substances (BLISs) with a strong anti‐H. pylori activity and some neutralized, catalase‐ and heat‐treated CFSs inhibited >83% of the test strains. Bacteriocin‐like inhibitory substance production of GLB strains can render them valuable probiotics in the control of H. pylori infection. (Source: Letters in Applied Microbiology)
Source: Letters in Applied Microbiology - October 23, 2017 Category: Microbiology Authors: L. Boyanova, G. Gergova, R. Markovska, D. Yordanov, I. Mitov Tags: Original Article Source Type: research
Editorial Board
(Source: Letters in Applied Microbiology)
Source: Letters in Applied Microbiology - October 16, 2017 Category: Microbiology Tags: Issue Information Source Type: research
Modified RS ‐LAMP assay and use of lateral flow devices for rapid detection of Leifsonia xyli subsp. xyli
This study presents a modified loop‐mediated isothermal amplification (LAMP) assay for the detection of Leifsonia xyli subsp. xyli. Modifications include incorporation of uracil nucleoside glycosylase to eliminate carry‐over contamination and substitution of colorimetric detection for the use of lateral flow devices. LAMP master mix was preprepared and was stably stored up to 4 months at −20°C. Sugarcane leaf sheaths worked well as a substitute to xylem sap as template, although the sensitivity was lower. These modifications allow the assay to be conducted without contamination concerns and reduction in set up time...
Source: Letters in Applied Microbiology - October 10, 2017 Category: Microbiology Authors: N. Naidoo, M. Ghai, K. Moodley, L. Mkize, L. Martin, S. McFarlane, S. Rutherford Tags: Original Article Source Type: research
Associations between resistance phenotype and gene expression in response to serial exposure to oxacillin and ciprofloxacin in Staphylococcus aureus
This study was designed to delineate the relationship between resistance phenotypes and gene expression in wild‐type (SAWT), oxacillin‐induced (SAOXA), ciprofloxacin‐induced (SACIP), and clinically‐acquired antibiotic‐resistant Staphylococcus aureus (SACA) exposed to oxacillin (β‐lactam) and ciprofloxacin (fluoroquinolone). The phenotypic response and gene expression were varied with the antibiotic exposure. SAWT was highly resistant to oxacillin (MIC=8 μg ml‐1) after serial exposure to oxacillin, while the oxacillin susceptibility was not changed in SAWT when exposed to ciprofloxacin (MIC=0.25 μg ml‐1)....
Source: Letters in Applied Microbiology - October 4, 2017 Category: Microbiology Authors: Md Jalal Uddin, Juhee Ahn Tags: Original Article Source Type: research
Novel screening strategy reveals a potent Bacillus antagonist capable of mitigating wheat take ‐all disease caused by Gaeumannomyces graminis var. tritici
This article is protected by copyright. All rights reserved. (Source: Letters in Applied Microbiology)
Source: Letters in Applied Microbiology - October 4, 2017 Category: Microbiology Authors: D. D. Zhang, X. J. Guo, Y. J. Wang, T. G. Gao, B. C. Zhu Tags: Original Article Source Type: research
Immunogenicity of a fusion protein containing PilQ and disulphide turn region of PilA from Pseudomonas aeruginosa in mice
In conclusion, our primary results revealed that the designed recombinant protein is a protective construct and may be used as a potential candidate for prophylactic purposes against P. aeruginosa infection.
Significance and Impact of the StudyIn this study we examined the potential of integrated PilQ/PilA (QA) antigen as a vaccine candidate against Pseudomonas aeruginosa. Nowadays, anti‐adhesion based vaccines are considered as new means to prevent or treat bacterial infections. Our study revealed that chimeric protein PilQ and disulphide turn region of PilA triggers production of specific antibodies. This humoral immun...
Source: Letters in Applied Microbiology - October 3, 2017 Category: Microbiology Authors: M. Gholami, A.S. Chirani, S. Razavi, R. Falak, G. Irajian Tags: Original Article Source Type: research
Effect of Orally Administered Vibrio Bacterin on Immunity, Survival and Growth in Tiger shrimp (Penaeus monodon) Grow out Culture Ponds
Abstract
Vibriosis is one of the important diseases causing economic loss to the shrimp industry worldwide. The present study reports field observations on the immune stimulatory effect of vibrio bacterin in commercial tiger shrimp (Penaeus monodon) grow out culture ponds (n=62) which were grouped under three stocking densities; low (6‐8 no.m−2), medium (9‐11 no.m−2) and high (12‐14 no.m−2). The bacterin was administered in feed as a top dressing at final concentration equivalent to 2x108 CFU kg−1 feed twice a week throughout the culture period. In twenty representative ponds total haemocyte count (THC) and p...
Source: Letters in Applied Microbiology - October 1, 2017 Category: Microbiology Authors: Arvind Kumar Ray, C. Gopal, H.G. Solanki, T. Ravisankar, P.K. Patil Tags: Original Article Source Type: research
Mannose and galactose as substrates for production of itaconic acid by Aspergillus terreus
This article is protected by copyright. All rights reserved. (Source: Letters in Applied Microbiology)
Source: Letters in Applied Microbiology - October 1, 2017 Category: Microbiology Authors: B. C. Saha, G. J. Kennedy Tags: Original Article Source Type: research
Bacteriocin ‐like inhibitory activities of seven Lactobacillus delbrueckii subspecies bulgaricus strains against antibiotic susceptible and resistant Helicobacter pylori strains
This article is protected by copyright. All rights reserved. (Source: Letters in Applied Microbiology)
Source: Letters in Applied Microbiology - October 1, 2017 Category: Microbiology Authors: Lyudmila Boyanova, Galina Gergova, Rumyana Markovska, Daniel Yordanov, Ivan Mitov Tags: Original Article Source Type: research
Effects of Bacillus fermentation on the protein microstructure and anti ‐nutritional factors of soybean meal
This article is protected by copyright. All rights reserved. (Source: Letters in Applied Microbiology)
Source: Letters in Applied Microbiology - October 1, 2017 Category: Microbiology Authors: Li Zheng, Da Li, Zhuolin Li, Lining Kang, Yuanyuan Jiang, Xiangying Liu, Yanping Chi, Yuqiu Li, Jinghui Wang Tags: Original Article Source Type: research
Rapid and simple method by combining FTA ™ card DNA extraction with the adaptation of a two set multiplex PCR for simultaneous detection of non‐O157 shiga‐toxin producing Escherichia coli strains and virulence genes from food samples
This article is protected by copyright. All rights reserved. (Source: Letters in Applied Microbiology)
Source: Letters in Applied Microbiology - September 27, 2017 Category: Microbiology Authors: Sun Ae Kim, Si Hong Park, Sang In Lee, Steven C. Ricke Tags: Original Article Source Type: research
