Calcitriol Ameliorates Kidney Injury Through Reducing Podocytopathy, Tubular Injury, Inflammation and Fibrosis in 5/6 Subtotal Nephrectomy Model in Rats.
Abstract Chronic kidney diseases (CKDs) lead to end-stage renal diseases (ESRD) which are characterized by glomerulosclerosis, tubular injury, anemia, inflammation, and interstitial fibrosis. Vitamin D is known to have renal protective effects. However, its effects relate to low and high doses of Vitamin D in CKD model is still unknown. CKD was performed using 5/6 subtotal nephrectomy procedure in male Sprague Dawley rats (3 months old, 200-300 grams, SN group; n=6), then rats were sacrificed on day 14 after operation. Sham operation was used for control (SO group; n=6). Calcitriol was administered in two doses : ...
Source: Kobe J Med Sci - March 27, 2020 Category: General Medicine Authors: Sari DCR, Putri MW, Leksono TP, Chairunnisa N, Reynaldi GN, Simanjuntak BC, Debora J, Yunus J, Arfian N Tags: Kobe J Med Sci Source Type: research

Current Situation of Chronic Kidney Disease Management in General Practice in Japan: A Questionnaire Survey for General Physicians.
Abstract Total management of chronic kidney disease has been well established, and the screening using dipstick urine test has already been widespread in Japan. Nevertheless, the number of dialysis patients is still rising. While clinical cooperation between general physicians and nephrologists is expected to improve prognoses of chronic kidney disease patients, real situation of the management in general practice has not been obvious. We conducted a questionnaire survey for the doctors of Hyogo Prefecture Medical Association excluding nephrologists to clarify the situation and the issue about chronic kidney disea...
Source: Kobe J Med Sci - March 27, 2020 Category: General Medicine Authors: Ito J, Nishi S Tags: Kobe J Med Sci Source Type: research

Nursing Care Time for Newborns during Hospitalization in a Mixed Hospital Ward with an Obstetrics Department.
CONCLUSION: The nursing care time for healthy newborns gradually decreased with the passage of time after birth. Healthy newborns should be treated as individuals, and the number of nursing staff should be adjusted according to the number of newborns in the ward to ensure nursing care quality and to prevent life-threatening events during the first 24 hours after birth. PMID: 32249271 [PubMed - in process] (Source: Kobe J Med Sci)
Source: Kobe J Med Sci - March 9, 2020 Category: General Medicine Authors: Nakai K, Saito I, Osawa K Tags: Kobe J Med Sci Source Type: research

Vitamin D Ameliorates Kidney Ischemia Reperfusion Injury via Reduction of Inflammation and Myofibroblast Expansion.
This study aimed to investigate the effect of vitamin D treatment on kidney IR injury in mice. Kidney IR injury was performed using 30 minutes of bilateral clamping of renal pedicles, then released in male Swiss Webster mice (3 months, 30-40 grams, n=20), which were divided into three groups: sham operation (SO) group, IR injury (IRI) group, and IR injury with 0.25 µg/ kg body weight of vitamin D treatment (IR7+VD). Mice were terminated at day 7 post operation, kidneys were harvested and used for paraffin making, immunostaining and RNA extraction. Tubular injury was quantified based on Periodic Acid-Schiff's (PAS) st...
Source: Kobe J Med Sci - March 9, 2020 Category: General Medicine Authors: Arfian N, Budiharjo S, Wibisono DP, Setyaningsih WAW, Romi MM, Saputri RLAAW, Rofiah EK, Rahmanti T, Agustin M, Sari DCR Tags: Kobe J Med Sci Source Type: research

The Affect of Lifestyle on Bone Mineral Density and Bone Turnover in Young Women.
Abstract INTRODUCTION: This research aimed to evaluate the effect of lifestyle factors such as nutrient intake and physical activity on bone mineral density (BMD) and bone turnover in young women. MATERIALS AND METHODS: BMD was assessed using Quantitative Ultrasound; lifestyle-related factors such as dietary habits, and physical activity were examined using questionnaires in 194 female college students. The biochemical markers of bone turnover were measured in the Osteopenia (BMD below the Young Adult Mean [YAM] -1.0SD, 16 subjects) and Normal (above the YAM-1.0SD, 31 subjects) groups. RESULTS: The perce...
Source: Kobe J Med Sci - February 28, 2020 Category: General Medicine Authors: Motooka N, Matsuo H Tags: Kobe J Med Sci Source Type: research

Factors Preventing Prolonged Closed-Suction Drain Placement after Immediate Breast Reconstruction with Tissue Expanders.
CONCLUSIONS: Our findings suggested that placing an extra-axillary closed-suction drain following axillary dissection, and reducing intraoperative bleeding and surgical trauma, could prevent prolonged drain placement in immediate breast reconstruction with tissue expanders. PMID: 32201428 [PubMed - in process] (Source: Kobe J Med Sci)
Source: Kobe J Med Sci - February 28, 2020 Category: General Medicine Authors: Tokiyoshi T, Tsunashima C, Nomura T, Hashikawa K, Terashi H, Kawamura S Tags: Kobe J Med Sci Source Type: research

Prevalence of Parasitic Contamination of Cockroaches Collected from Fresh Markets in Chachoengsao Province, Thailand.
This study aimed to determine the prevalence and species type of human pathogenic parasite infestations on the external body surface of the cockroaches. A total of 450 cockroaches collected from three fresh markets were identified in three species belonging to Periplaneta americana, Periplaneta brunnea and Periplaneta australasiae. Helminth and protozoan species were identified by using a direct wet smear technique and modified acid-fast staining technique. The overall prevalence rate of parasitic infestation on the external body surface of cockroaches was 46.4% (209/450). The number of cockroaches infested with protozoa w...
Source: Kobe J Med Sci - February 10, 2020 Category: General Medicine Authors: Dokmaikaw A, Suntaravitun P Tags: Kobe J Med Sci Source Type: research

Fatal Hemoptysis Due to Endobronchial Aspergilloma in the Hyperinflated Native Lung after Single-Lung Transplantation for Lymphangioleiomyomatosis: A Case Report.
Abstract Pulmonary lymphangioleiomyomatosis accounts for the majority of cadaveric lung transplantation cases. Post-transplantation management is continuingly necessary not only to prevent the progression of LAM but also to address complications. A woman with lymphangioleiomyomatosis underwent cadaveric lung transplantation. She developed post-operative native lung hyperinflation and hemoptysis with cavity shadow in the native lung on computed tomography. Isolated Aspergillus from her sputum and positive Aspergillus galactomannan antigen in the blood led to a diagnosis of aspergillosis. Despite the reduction of he...
Source: Kobe J Med Sci - January 20, 2020 Category: General Medicine Authors: Yoshizaki A, Yamamoto M, Hirabayashi A, Ono Y, Hatakeyama Y, Nakata K, Tamura D, Tachihara M, Kamiryo H, Kobayashi K, Nishio M, Nishimura Y Tags: Kobe J Med Sci Source Type: research

Loss of Family with Sequence Similarity 13, Member A Exacerbates Pulmonary Fibrosis Potentially by Promoting Epithelial to Mesenchymal Transition.
Abstract Idiopathic pulmonary fibrosis (IPF) is a devastating disease with poor prognosis due to limited clinical treatment options. IPF is characterized by the augmented deposition of extracellular matrix driven by myofibroblasts, and the epithelial-mesenchymal transition (EMT) has been known to play an essential role in the mechanism of pulmonary fibrosis. Previous genome-wide association study identified Fam13a as one of genes that showed genetic link with IPF and chronic obstructive pulmonary disease. Here, we analyzed the role of Fam13a in the pathogenesis of pulmonary fibrosis using Fam13a-deficient mice. We...
Source: Kobe J Med Sci - January 20, 2020 Category: General Medicine Authors: Rahardini EP, Ikeda K, Nugroho DB, Hirata KI, Emoto N Tags: Kobe J Med Sci Source Type: research

Isolated Nerve Grafting for a Young Patient with a Complete Common Peroneal Nerve Palsy Following a Traumatic Knee Dislocation: A case report.
We report a case of complete CPN palsy following a knee dislocation treated with sural nerve grafting. CASE: A 19-year-old man suffered a knee injury during a hurdle race. Diagnosis in a previous hospital revealed a complex ligament injury with CPN palsy. Ten weeks following injury, he was admitted to our institution because of a lack of neurological improvement. Considering the grade 0 results obtained in the manual muscle test (MMT) of tibialis anterior (TA) and extensor hallucis longus (EHL), the patient was diagnosed with complete neurotmesis of CPN, and surgery was performed. Operative findings revealed CPN disco...
Source: Kobe J Med Sci - January 20, 2020 Category: General Medicine Authors: Mukohara S, Inui A, Mifune Y, Nishimoto H, Kataoka T, Kurosawa T, Yamaura K, Kuroda R Tags: Kobe J Med Sci Source Type: research

Anesthetic Management of Transcatheter Aortic Valve Replacement under Extracorporeal Membrane Oxygenation in a Patient with Acute Decompensated Heart Failure: A Case Report.
Abstract We managed general anesthesia for transcatheter aortic valve replacement (TAVR) under elective extracorporeal membrane oxygenation (ECMO) in a patient with aortic valve stenosis (AS) complicated with acute decompensated heart failure. The patient was an 87-year-old woman with acute heart failure due to severe AS who had been hospitalized. However, her low cardiac output did not improve, and weaned her off catecholamines was difficult, so semi-urgent TAVR was performed. Due to her acute decompensated heart failure complicated by low-left ventricular function, we decided electively to use ECMO for transfemo...
Source: Kobe J Med Sci - November 14, 2019 Category: General Medicine Authors: Okada T, Yoshida T, Makino S, Obata N, Mizobuchi S Tags: Kobe J Med Sci Source Type: research

Newborn Screening for Spinal Muscular Atrophy: DNA Preparation from Dried Blood Spot and DNA Polymerase Selection in PCR.
CONCLUSION: Direct PCR with DNA polymerases like KOD FX NeoTM has potential to be widely used in SMA newborn screening in the near future as it obviates the DNA extraction process from DBS and can precisely amplify the target sequences in spite of the presence of PCR inhibitors. PMID: 32029694 [PubMed - in process] (Source: Kobe J Med Sci)
Source: Kobe J Med Sci - November 14, 2019 Category: General Medicine Authors: Takeuchi A, Tode C, Nishino M, Wijaya YOS, Niba ETE, Awano H, Takeshima Y, Saito T, Saito K, Lai PS, Bouike Y, Nishio H, Shinohara M Tags: Kobe J Med Sci Source Type: research

Lifestyle of Patients with Alcoholic Liver Disease and Factors Leading to Hospital Readmission: A Prospective Observational Study.
The objective of this study was to clarify the lifestyle characteristics of patients with alcoholic liver disease (ALD) who were readmitted to the hospital, and to identify the background factors associated with these characteristics. This was a prospective observational study. Over a period of 3 months following hospital discharge, we conducted structured interviews to investigate the following five lifestyle characteristics based on our previous research: dietary intake, alcohol consumption or abstinence, psycho-emotional status, regularity of life habits, adherence to treatment. We also collected data on background fact...
Source: Kobe J Med Sci - November 12, 2019 Category: General Medicine Authors: Park SM, Saito N, Kim SR, Miyawaki I Tags: Kobe J Med Sci Source Type: research

Antiviral Activity of Cananga odorata Against Hepatitis B Virus.
In conclusion, the C. odorata extract could be a good candidate for the development of anti-HBV drugs. PMID: 31956259 [PubMed - in process] (Source: Kobe J Med Sci)
Source: Kobe J Med Sci - November 12, 2019 Category: General Medicine Authors: Indrasetiawan P, Aoki-Utsubo C, Hanafi M, Hartati S, Wahyuni TS, Kameoka M, Yano Y, Hotta H, Hayashi Y Tags: Kobe J Med Sci Source Type: research

Study on the Language Formation Process of Very-Low-Birth-Weight Infants in Infancy Using a Formant Analysis.
Abstract Expressive language development depends on anatomical factors, such as motor control of the tongue and oral cavity needed for vocalization, as well as cognitive aspects for comprehension and speech. The purpose of this study was to examine the differences in expressive language development between normal-birth-weight (NBW) infants and very-low-birth-weight (VLBW) infants in infancy using a formant analysis. We also examined the presence of differences between infants with a normal development and those with a high risk of autism spectrum disorder who were expected to exist among VLBW infants. The particip...
Source: Kobe J Med Sci - November 8, 2019 Category: General Medicine Authors: Maebayashi H, Takiguchi T, Takada S Tags: Kobe J Med Sci Source Type: research

Spinal Muscular Atrophy: New Screening System with Real-Time mCOP-PCR and PCR-RFLP for SMN1 Deletion.
CONCLUSION: In our new system for detection of SMN1 deletion, real-time mCOP-PCR rapidly proved the presence or absence of SMN1 and SMN2, and the results were easily tested by PCR-RFLP. This solid genotyping system will be useful for SMA screening. PMID: 31956255 [PubMed - in process] (Source: Kobe J Med Sci)
Source: Kobe J Med Sci - July 16, 2019 Category: General Medicine Authors: Niba ETE, Rochmah MA, Harahap NIF, Awano H, Morioka I, Iijima K, Takeshima Y, Saito T, Saito K, Takeuchi A, Lai PS, Bouike Y, Matsuo M, Nishio H, Shinohara M Tags: Kobe J Med Sci Source Type: research

Spinal Muscular Atrophy: Advanced Version of Screening System with Real-Time mCOP-PCR and PCR-RFLP for SMN1 Deletion.
CONCLUSION: In the advanced version of our screening system for homozygous SMN1 deletion using DBS, the real-time mCOP-PCR with newly-designed reverse primers demonstrated the presence or absence of SMN1 and SMN2 within a shorter time, and the results were easily tested by PCR-RFLP. This rapid and accurate screening system will be useful for detection of newborn infants with SMA. PMID: 31956256 [PubMed - in process] (Source: Kobe J Med Sci)
Source: Kobe J Med Sci - July 16, 2019 Category: General Medicine Authors: Niba ETE, Rochmah MA, Harahap NIF, Awano H, Morioka I, Iijima K, Takeshima Y, Saito T, Saito K, Takeuchi A, Lai PS, Bouike Y, Matsuo M, Nishio H, Shinohara M Tags: Kobe J Med Sci Source Type: research

Nested PCR Amplification Secures DNA Template Quality and Quantity in Real-time mCOP-PCR Screening for SMA.
CONCLUSION: In our study on the real-time mCOP-PCR screening system for SMA, a nested PCR secured the DNA template quality and quantity, leading to unambiguous results of SMA screening. PMID: 31956257 [PubMed - in process] (Source: Kobe J Med Sci)
Source: Kobe J Med Sci - July 16, 2019 Category: General Medicine Authors: Wijaya YOS, Niba ETE, Rochmah MA, Harahap NIF, Awano H, Takeshima Y, Saito T, Saito K, Takeuchi A, Lai PS, Bouike Y, Nishio H, Shinohara M Tags: Kobe J Med Sci Source Type: research

Red LED Light Is Influenced by IL-6 to Promote the Migration Ability of Oral Squamous Cell Carcinoma Cell Line.
CONCLUSIONS: HSC-3 cells irradiated with red LED light showed increased migration ability. Interestingly, we clarified that the expression of Interleukin-6 (IL-6), which promotes the migratory ability of cancer cells, is induced. PMID: 31327864 [PubMed - in process] (Source: Kobe J Med Sci)
Source: Kobe J Med Sci - July 10, 2019 Category: General Medicine Authors: Matsuo K, Suzuki H, Yatagai N, Enomoto Y, Kitayama M, Shigeoka M, Kimoto A, Matsumoto K, Komori T Tags: Kobe J Med Sci Source Type: research

Regulation of Small Intestinal Epithelial Homeostasis by Tsc2-mTORC1 Signaling.
Abstract Mammalian target of rapamycin complex 1 (mTORC1), a protein complex containing the serine/threonine kinase mTOR, integrates various growth stimulating signals. mTORC1 is expressed in intestinal epithelial cells (IECs), whereas the physiological roles of this protein complex in homeostasis of IECs remain virtually unknown. We here generated mice, in which tuberous sclerosis complex 2 (Tsc2), a negative regulator of mTORC1, was specifically ablated in IECs (Tsc2 CKO mice). Ablation of Tsc2 enhanced the phosphorylation of mTORC1 downstream molecules such as ribosomal S6 protein and 4E-BP1 in IECs. Tsc2 CKO m...
Source: Kobe J Med Sci - April 12, 2019 Category: General Medicine Authors: Setiawan J, Kotani T, Konno T, Saito Y, Murata Y, Noda T, Matozaki T Tags: Kobe J Med Sci Source Type: research

Neonatal Alloimmune Thrombocytopenia: A Report of Four Cases.
Abstract Antibodies against fetal platelet alloantigens in maternal blood cause neonatal alloimmune thrombocytopenia (NAIT). We encountered four newborns with NAIT from three women. A woman carried anti-human platelet antigen (HPA)-1a antibody, and vaginally delivered a newborn who had subarachnoid hemorrhage and platelet transfusions. She delivered the second newborn by a cesarean section who had no symptom. The second woman carried anti-human leukocyte antigen-A2 antibody and vaginally delivered a newborn who had no symptom. The third woman with a history of recurrent pregnancy losses carried anti-HPA-4b antibod...
Source: Kobe J Med Sci - March 5, 2019 Category: General Medicine Authors: Uenaka M, Morizane M, Tanimura K, Deguchi M, Ebina Y, Hashimoto M, Morioka I, Yamada H Tags: Kobe J Med Sci Source Type: research

The Influence of Chronotype and Working Condition on Sleep Status and Health Related Quality of Life of Daytime Office Workers.
Abstract Several companies in Japan introduced early working conditions (including recommendations on early morning work and prohibitions on nighttime overtime work) to decrease the number of long working hours at night. Nevertheless, individuals possess their own chronotype, i.e., their behavioral timing preference-be it morning or evening-that is associated with worker health. The purpose of this study was to investigate the influence of chronotype and working conditions on sleep and health related quality of life (HRQOL) using 126 daytime office workers who were classified as morning or evening type by their Mo...
Source: Kobe J Med Sci - March 5, 2019 Category: General Medicine Authors: Sasawaki Y, Shiotani H Tags: Kobe J Med Sci Source Type: research

Investigation of the Treatment and Living Assistance Needed by Patients with Young-Onset Parkinson's Disease.
This study assessed the symptoms, treatment, social systems use, and perception of living conditions of patients with young-onset Parkinson's disease (YOPD), and investigated the support needed by them. METHOD: Among the 252 people who completed our questionnaire, we defined YOPD patients as those diagnosed as young onset or those with onset at ≤40 years. The data were compared with others. RESULTS: There were 24 patients with YOPD (9.5%) (average age: 61.7 years), with an average disease duration 6.4 years longer (p
Source: Kobe J Med Sci - February 21, 2019 Category: General Medicine Authors: Iwasa Y, Saito I, Fujii C Tags: Kobe J Med Sci Source Type: research

Questionnaire Survey for Bronchial Asthma in Elderly Care Facilities.
Abstract In developed countries such as North America, the decline in mortality from bronchial asthma has ceased since 2006. The decline in mortality rate is also decreasing in Japan, where about 1,500 asthma deaths have been reported. Among these, elderly people aged 65 years or over account for about 90% of cases. Therefore, the treatment of elderly patients with asthma is an important subject. However, few studies have been conducted on asthma in elderly patients. In this survey, we distributed a questionnaire to 253 elderly care facilities in Kobe, Japan. Ninety facilities responded, and 223 patients in 70 out...
Source: Kobe J Med Sci - February 13, 2019 Category: General Medicine Authors: Shinke H, Kamiryo H, Umezawa K, Hori S, Nakata K, Nagano T, Hazeki N, Kobayashi K, Fukabori T, Nishimura Y Tags: Kobe J Med Sci Source Type: research

Emotional and Behavioral Problems of Pre-school Children with Autistic Spectrum Disorder Assessed by the Child Behavior Checklist 1 ½-5.
Emotional and Behavioral Problems of Pre-school Children with Autistic Spectrum Disorder Assessed by the Child Behavior Checklist 1½-5. Kobe J Med Sci. 2019 Jan 22;64(5):E170-E173 Authors: Irwanto, Kahfi M, Febriyana N, Hartini S, Takada S Abstract BACKGROUND: Children with Autism Spectrum Disorder (ASD) experience a significant disruption in the ability to communicate and interact socially. Early detection is important; the earlier the child is identified with ASD, the faster the intervention can begin. The Child Behavior Checklist (CBCL) is a screening tool capable of identifying various emot...
Source: Kobe J Med Sci - January 22, 2019 Category: General Medicine Authors: Irwanto, Kahfi M, Febriyana N, Hartini S, Takada S Tags: Kobe J Med Sci Source Type: research

Mothers'Breastfeeding-Related Durations and Nursing Management During the Early Postpartum Period in a Mixed Hospital Ward with an Obstetrics Department: A Prospective Observational Study.
CONCLUSION: Mothers spent 20% of their postpartum hospitalization period for BF-related activities. Individual support is necessary, especially for primiparous mothers, who spent more time than multiparous mothers for BF-related activities on postpartum day 4. In a mixed ward, postpartum mothers need a comfortable hospital environment in which midwives can dedicate themselves to mother-child dyad care. PMID: 30988262 [PubMed - in process] (Source: Kobe J Med Sci)
Source: Kobe J Med Sci - January 17, 2019 Category: General Medicine Authors: Mitsui Y, Saito I Tags: Kobe J Med Sci Source Type: research

Fetal Primary Small Bowel Volvulus Associated with Acute Gastric Dilatation Detected by Ultrasonography.
In conclusion, acute gastric dilatation detected by fetal ultrasound can indicate the fetal intestinal volvulus. PMID: 30728342 [PubMed - in process] (Source: Kobe J Med Sci)
Source: Kobe J Med Sci - January 11, 2019 Category: General Medicine Authors: Fukushima S, Fujioka K, Ashina M, Ohyama S, Ikuta T, Nishida K, Miyauchi H, Okata Y, Bitoh Y, Tanimura K, Deguchi M, Yamada H, Iijima K Tags: Kobe J Med Sci Source Type: research

Morphometric Measurements of the Hip Bone in Turkish Adult Population.
i M, Unur E Abstract OBJECTIVES: Coxal bone paticipates in the formation of the pelvic skeleton. Anatomy knowledge on coxafemoral joint as well as careful history taking and physical examination are crucial in evaluation and management of disorders involving hip joint. The aims of the present study were to perform morphometric measurements of the human coxal bones, calculation of their articular surface areas and report the range of these parameters regarding Turkish adult population. METHODS: Seventy-two dry human adult coxal bones (39 left and 33 right) from the Anatomy Departments of Erciyes University, In...
Source: Kobe J Med Sci - December 10, 2018 Category: General Medicine Authors: Demir M, Atay E, Güneri B, Yilmaz H, Arpaci MF, Güler HS, Al Ö, Ertekin T, Nisari M, Unur E Tags: Kobe J Med Sci Source Type: research

The Transition of Sleep Behaviors in Twin Infants and Their Mothers in Early Infancy.
CONCLUSION: This research revealed the transition of sleep behaviors in twin infants and their mothers in early infancy. Cobedding may facilitate more synchronized sleep states of twin infants. PMID: 30728338 [PubMed - in process] (Source: Kobe J Med Sci)
Source: Kobe J Med Sci - December 4, 2018 Category: General Medicine Authors: Kondo C, Takada S Tags: Kobe J Med Sci Source Type: research

An Open-Label, Multi-Institutional, Randomized Study to Evaluate the Additive Effect of a Leukotriene Receptor Antagonist on Cough Score in Patients with Cough-Variant Asthma Being Treated with Inhaled Corticosteroids.
Abstract Cough-variant asthma is one of the most common reasons for chronic cough. It is important to treat appropriately cough-variant asthma because 30% to 40% of cough-variant asthma becomes a typical asthma. However, little is known about the treatment of cough-variant asthma except for inhaled corticosteroid (ICS). The aim of this study was to validate the additive efficacy of a leukotriene receptor antagonist (LTRA) on cough score and respiratory function in patients with cough-variant asthma being treated with ICS. A total 28 patients were randomly assigned to either an ICS + LTRA group or an ICS group. The...
Source: Kobe J Med Sci - December 4, 2018 Category: General Medicine Authors: Miwa N, Nagano T, Ohnishi H, Nishiuma T, Takenaka K, Shirotani T, Nakajima T, Dokuni R, Kawa Y, Kobayashi K, Funada Y, Kotani Y, Nishimura Y Tags: Kobe J Med Sci Source Type: research

Short-Term and Long-Term Outcomes of Infliximab and Tacrolimus Treatment for Moderate to Severe Ulcerative Colitis: Retrospective Observational Study.
CONCLUSION: This study indicates that IFX and TAC produce similar short-term outcomes in UC patients, but IFX produces better long-term outcomes than TAC especially with avoidance of colectomy. Our data suggest that IFX therapy may be prioritized over TAC for the treatment of moderate to severe UC. PMID: 30728340 [PubMed - in process] (Source: Kobe J Med Sci)
Source: Kobe J Med Sci - December 4, 2018 Category: General Medicine Authors: Otsuka T, Ooi M, Tobimatsu K, Wakahara C, Watanabe D, Adachi S, Yasutomi E, Yamairi H, Ku Y, Yoshida M, Hoshi N, Kodama Y Tags: Kobe J Med Sci Source Type: research

Case Series of Comminuted Olecranon Fracture Treated by Plate Fixation; Do We Have to Remove the Plate?
Abstract Comminuted olecranon fracture requires surgical intervention. Plate fixation has been performed on the majority of cases. We reviewed the cases of comminuted olecranon fracture in young and middle age treated by plate osteosynthesis and analyzed the functional outcome, complications and ratio and timing of hardware removal. Fifteen cases of comminuted olecranon fractures treated by plate fixation were reviewed. Bone union was achieved in all cases, the average range of motion at the final follow up was -11° in extension, 133° in flexion, 89° in pronation and 88° in supination. Hardware rem...
Source: Kobe J Med Sci - November 12, 2018 Category: General Medicine Authors: Inui A, Kuroda T, Kurosawa T, Kokubu T, Mifune Y, Nishimoto H, Kuroda R Tags: Kobe J Med Sci Source Type: research

Deletion Polymorphism of Angiotensin-Converting Enzyme Gene Is Associated with Low Muscle Mass in Elderly People in Jakarta, Indonesia.
This study was conducted to determine the association between ACE I/D polymorphism and muscle mass in elderly people. A total of 130 elderly people were recruited from nursing homes in Jakarta. Anthropometric components affecting the muscle mass were examined. Cross-sectional analyses were performed to compare data using t-test, ANOVA and ANCOVA, and linear regression. Genotyping of the ACE I/D polymorphisms was performed by PCR methods, and muscle mass was evaluated by BIA. Genotype distribution counts II 65.38%, ID 13.85%, and DD 20.77% were not consistent with the Hardy-Weinberg equilibrium (χ² = 22.2, df = 2; p
Source: Kobe J Med Sci - November 12, 2018 Category: General Medicine Authors: Handayani MDN, Sadewa AH, Farmawati A, Rochmah W Tags: Kobe J Med Sci Source Type: research

Reduction of Serum Uric Acid Associated with Attenuation of Renal Injury, Inflammation and Macrophages M1/M2 Ratio in Hyperuricemic Mice Model.
This study aimed to look at the effect of lowering serum uric acid levels on renal injury in mice. METHODS: This study used 25 male mice, 3 months old, that divided into 5 groups. We injected uric acid intraperitoneally, 125mg/kg/day for 7 days (UA7) and 14 days (UA14), to induce hyperuricemia and then gave allopurinol 50mg/kg/day for 7 days to lower serum uric acid levels (UA7AL7 and UA14AL7). At the end of the treatment, we measured serum uric acid levels, Glomerular Injury Score (GIS) and Arteriolar Injury Score (AIS) with PAS staining, eNOS and MCP-1 expression with Reverse Transcriptase-PCR (RT-PCR), macrophages ...
Source: Kobe J Med Sci - October 29, 2018 Category: General Medicine Authors: Haryono A, Nugrahaningsih DAA, Sari DCR, Romi MM, Arfian N Tags: Kobe J Med Sci Source Type: research

Potential of Coffee Fruit Extract and Quinic Acid on Adipogenesis and Lipolysis in 3T3-L1 Adipocytes.
This study was to assess the impact of different colors of coffee fruit (green, yellow and red) on adipogenesis and/or lipolysis using 3T3-L1 adipocytes. Characterization of chemical constituents in different colors of coffee fruit extracts was determined by ESI-Q-TOF-MS. The cytotoxicity of the extracts in 3T3-L1 preadipocytes were evaluated by MTT assay. Oil-red O staining and amount of glycerol released in 3T3-L1 adipocytes were measured for lipid accumulation and lipolysis activity. All coffee fruit extracts displayed similar chromatographic profiles by chlorogenic acid> caffeoylquinic acid> caffeic acid. Differe...
Source: Kobe J Med Sci - October 15, 2018 Category: General Medicine Authors: Duangjai A, Nuengchamnong N, Suphrom N, Trisat K, Limpeanchob N, Saokaew S Tags: Kobe J Med Sci Source Type: research

Spatial Memory Disturbance Following Transient Brain Ischemia is Associated with Vascular Remodeling in Hippocampus.
Abstract A number of studies have investigated the effects of ischemic injury on functional and cellular characteristics of hippocampus. There is only a limited study on vascular remodeling of it. The present study aimed at examining vascular remodeling in hippocampus and spatial memory disturbances after transient brain ischemia. Male Wistar rats were randomly divided into four groups, i.e. sham operated (SHAM), transient brain ischemia with 1 day reperfusion (IR1), 3 day reperfusion (IR3), and 10 days reperfusion (IR10) groups. Transient brain ischemia was induced by bilateral common carotid artery occlusion (BC...
Source: Kobe J Med Sci - October 15, 2018 Category: General Medicine Authors: Hermawati E, Arfian N, Mustofa, Partadiredja G Tags: Kobe J Med Sci Source Type: research

Uncoupling Protein 2 (UCP2) as Genetic Risk Factor for Obesity in Indonesia is Different in Gender Stratification.
This study consisted of 200 Indonesian subjects of Javanese ethnicity consisting of 100 obese and 100 non-obese participants. Examination of Ala55Val (C> T) UCP2 genotype used Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) methods and 45 bp I/D genotype used PCR methods. Polymorphism of Ala55Val UCP2 genotype in the male group showed that TT genotype and T allele significantly lowers the risk of obesity. Insertion/deletion of 45 bp UCP2 gene in the male group showed that II genotype and I allele significantly increase the risk of obesity whereas for women it showed that the DI genotype and...
Source: Kobe J Med Sci - October 15, 2018 Category: General Medicine Authors: Surniyantoro HNE, Sadewa AH, Hastuti P Tags: Kobe J Med Sci Source Type: research

Anti-inflammatory Effects of Hibiscus Sabdariffa Linn. on the IL-1 β/IL-1ra Ratio in Plasma and Hippocampus of Overtrained Rats and Correlation with Spatial Memory.
Anti-inflammatory Effects of Hibiscus Sabdariffa Linn. on the IL-1β/IL-1ra Ratio in Plasma and Hippocampus of Overtrained Rats and Correlation with Spatial Memory. Kobe J Med Sci. 2018 Oct 15;64(2):E73-E83 Authors: Bayani GFE, Marpaung NLE, Simorangkir DAS, Sianipar IR, Ibrahim N, Kartinah NT, Mansur IG, Purba JS, Ilyas EII Abstract Overtraining leads to an increase in IL-1β systemically due to muscle microtrauma, which affects the hippocampus, an important structure in spatial memory consolidation. The administration of Hibiscus sabdariffa Linn is expected to decrease IL-1β and increas...
Source: Kobe J Med Sci - October 15, 2018 Category: General Medicine Authors: Bayani GFE, Marpaung NLE, Simorangkir DAS, Sianipar IR, Ibrahim N, Kartinah NT, Mansur IG, Purba JS, Ilyas EII Tags: Kobe J Med Sci Source Type: research

Impact of Cytochrome P450 2C19 Reduced-Function Polymorphism on Lesions and Clinical Outcome in Japanese Patients After Drug-eluting Stent Implantation.
Abstract We previously reported that the cytochrome P450 (CYP) 2C19 reduced-function polymorphism was associated with decreased responsiveness to clopidogrel and intra-stent thrombus formation, as well as subsequent ischemic events after drug-eluting stent (DES) implantation. However, the relationship between the polymorphism and bleeding events remains unclear. Among 1427 consecutive patients who underwent DES implantation at Kobe University Hospital, 247 patients (341 lesions) were enrolled for this prospective observational study. All patients underwent follow-up optical coherence tomography (OCT) at 8 months a...
Source: Kobe J Med Sci - September 18, 2018 Category: General Medicine Authors: Tahara N, Shinke T, Otake H, Nishio R, Konishi A, Hirata KI Tags: Kobe J Med Sci Source Type: research

Endometrial Stromal Sarcoma Arising from Endometrial Polyp: A Case Report.
In conclusion, immunohistochemical analyses are useful for make an accurate diagnosis of ESS showing a transition from low-grade ESS to high-grade ESS in addition to the conventional method. PMID: 30381725 [PubMed - in process] (Source: Kobe J Med Sci)
Source: Kobe J Med Sci - September 11, 2018 Category: General Medicine Authors: Sato S, Ojima Y, Kanda M, Kizaki T, Ohara N Tags: Kobe J Med Sci Source Type: research

Docosahexaenoic Acid Reduces Palmitic Acid-Induced Endoplasmic Reticulum Stress in Pancreatic Β Cells.
In this study, we have established a system that can sensitively detect ER stress levels of living cells in a short period of time. This system can be used to monitor the state of the ER in living cells and lead to the investigation of the significance of physiological or pathological ER stress levels. PMID: 30381726 [PubMed - in process] (Source: Kobe J Med Sci)
Source: Kobe J Med Sci - September 11, 2018 Category: General Medicine Authors: Suzuki E, Matsuda T, Kawamoto T, Takahashi H, Mieda Y, Matsuura Y, Takai T, Kanno A, Koyanagi-Kimura M, Asahara SI, Inoue H, Ogawa W, Kido Y Tags: Kobe J Med Sci Source Type: research

Hyperbilirubinemia in Term Newborns Needing Phototherapy within 48 Hours after Birth in a Japanese Birth Center.
CONCLUSIONS: Altogether, data from this Japanese healthy birth center suggest that many apparently healthy newborns with or without excessive weight loss develop early-onset hyperbilirubinemia. In the 1992 Kobe University phototherapy treatment criterion, TSB, not UB, was the main index used to make treatment decisions in these patients. PMID: 30282894 [PubMed - in process] (Source: Kobe J Med Sci)
Source: Kobe J Med Sci - September 11, 2018 Category: General Medicine Authors: Tsujimae S, Yoshii K, Yamana K, Fujioka K, Iijima K, Morioka I Tags: Kobe J Med Sci Source Type: research

Exploring a Novel Screening Method for Patients with Oral Squamous Cell Carcinoma: A plasma Metabolomics Analysis.
CONCLUSIONS: The findings of this study suggest four potential metabolites as biomarkers for OSCC screening. PMID: 30282895 [PubMed - in process] (Source: Kobe J Med Sci)
Source: Kobe J Med Sci - September 11, 2018 Category: General Medicine Authors: Enomoto Y, Kimoto A, Suzuki H, Nishiumi S, Yoshida M, Komori T Tags: Kobe J Med Sci Source Type: research

Associated Factors of Psychological Distress among Japanese NICU Nurses in Supporting Bereaved Families Who Have Lost Children.
CONCLUSION: Clarifying the coping methods for psychological distress in supporting bereaved families may be necessary, and nurses need to identify appropriate coping methods. In nursing education, information on psychological distress related to children's deaths and bereavement care should be conveyed from the early stage and nurses must obtain preliminary knowledge. The creation of a bereavement follow-up system is recommended. PMID: 30282893 [PubMed - in process] (Source: Kobe J Med Sci)
Source: Kobe J Med Sci - June 6, 2018 Category: General Medicine Authors: Kitao M, Setou N, Yamamoto A, Takada S Tags: Kobe J Med Sci Source Type: research

Ultrasound and MRI Findings of Twin Pregnancies with Complete Hydatidiform Mole and Coexisting Normal Fetus: Two Case Reports.
CONCLUSION: MRI was useful for an accurate diagnosis for CHMCF. PMID: 30282891 [PubMed - in process] (Source: Kobe J Med Sci)
Source: Kobe J Med Sci - May 28, 2018 Category: General Medicine Authors: Imafuku H, Miyahara Y, Ebina Y, Yamada H Tags: Kobe J Med Sci Source Type: research

The Correlation Between Length of Work and Nasal Mucociliary Transport Time of Gas/Fuel Station Workers.
This study used a cross sectional design. The data were obtained from anamnesis, physical and NMCTT examinations of gas station workers in Yogyakarta, during November 2013. Inclusion criteria were: 1) 18-55 years old, 2) free of nasal topical medication for 10 days. Exclusion criterion: 1) acute rhinitis, 2) chronic sinusitis, 3) ever had nasal trauma, 4) ever had nasal surgery, 5) allergic rhinitis, and/or 6) septal deviation. Correlation analyses between length of work and NMCTT of gas station workers used Spearman correlation test with α 5% level of significance (p
Source: Kobe J Med Sci - May 28, 2018 Category: General Medicine Authors: Rianto BUD, Yudhanto D, Herdini C Tags: Kobe J Med Sci Source Type: research

Essential Role of Sphingosine Kinase 2 in the Regulation of Cargo Contents in the Exosomes from K562 Cells.
Abstract Sphingosine 1-phosphate (S1P) is a bioactive phosphorylated product of sphingosine catalyzed by sphingosine kinase (SphK) and implicated in diverse cellular functions including vesicular trafficking. In the present study we have shown the importance of one of the subtypes of SphK, SphK2, in the regulation of cargo content in exosomes released from human myeloid leukemia K562 cells. First, SphK2 has been shown to localize with N-Rh-PE-positive late endosomes in the cells. Next, siRNA-mediated knockdown of Sphk2 but not SphK1 resulted in a reduction of cargo content in purified exosomes. The involvement of ...
Source: Kobe J Med Sci - May 25, 2018 Category: General Medicine Authors: Mohamed NNI, Okada T, Kajimoto T, Nakamura SI Tags: Kobe J Med Sci Source Type: research

Nontypeable Haemophilus Influenzae Sepsis in a Term Neonate.
In conclusion, clinicians should consider NTHi as a cause of neonatal sepsis, even in term infants. PMID: 29955021 [PubMed - in process] (Source: Kobe J Med Sci)
Source: Kobe J Med Sci - April 19, 2018 Category: General Medicine Authors: Okubo S, Fujioka K, Yamane M, Ashina M, Fukushima S, Ikuta T, Ohyama S, Yamana K, Kobayashi A, Iijima K, Morioka I Tags: Kobe J Med Sci Source Type: research

A Case of Congenital Complete Atrioventricular Block Treated with Transdermal Tulobuterol.
Abstract Congenital complete atrioventricular block (CCAVB) is a condition in which the atria and ventricles beat independently of each other. CCAVB cases require permanent pacemaker implantation until adulthood. Nevertheless, consensus regarding postnatal medical therapy for bradycardia has not been reached. Here we report the case of a newborn with CCAVB, whose intractable bradycardia was successfully treated with transdermal tulobuterol. Tulobuterol is a selective β2-adrenoceptor agonist, widely used safely as bronchodilator in children. It also has positive inotropic and chronotropic effect via β1-ad...
Source: Kobe J Med Sci - April 19, 2018 Category: General Medicine Authors: Ikuta T, Fujioka K, Sato Y, Ashina M, Fukushima S, Ohyama S, Okubo S, Yamana K, Morizane M, Tanimura K, Deguchi M, Iijima K, Morioka I, Yamada H Tags: Kobe J Med Sci Source Type: research

Modified TALK Score for Japanese Patients with Laryngeal and Hypopharyngeal Cancers to Predict the Possibility of Laryngeal Preservation by Concurrent Chemoradiotherapy.
In this study, we validated the original TALK score in our clinical setting and modified the score for Japanese population. METHODS: The subjects were 21 patients with laryngeal cancer and 50 patients with hypopharyngeal cancer who underwent CCRT at Kobe University Hospital between April 2007 and August 2012. Data regarding T stage, serum albumin level just before treatment, maximum alcohol use, Karnofsky Performance Status (KPS), 3-year overall survival rate and 3-year larynx-preservation rate were collected from medical records. In the revised TALK score (JTALK), the following scoring criteria were modified to adjus...
Source: Kobe J Med Sci - April 19, 2018 Category: General Medicine Authors: Otsuki N, Matsui M, Takahashi M, Sasaki R, Kiyota N, Usami M, Nibu KI Tags: Kobe J Med Sci Source Type: research