Toxicological effects of bioactive peptide fractions obtained from Bothrops jararaca snake venom on the structure and function of mouse seminiferous epithelium
Conclusions: The major finding of the present study was that the LMWF inhibited spermatozoa production; principally in the spermiogenesis stage without altering claudin-1 distribution in the basal compartment. Moreover, NO increased by LMWF induce open of complexes junctions and release the germ cells of the adluminal compartment to the seminiferous tubule. (Source: Journal of Venomous Animals and Toxins including Tropical Diseases)
Source: Journal of Venomous Animals and Toxins including Tropical Diseases - June 23, 2020 Category: Tropical Medicine Source Type: research

Chronic venous ulcers: a review on treatment with fibrin sealant and prognostic advances using proteomic strategies
Abstract Venous ulcers are the main causes of chronic lower-limb ulcers. The healing difficulties encourage the research and development of new products in order to achieve better therapeutic results. Fibrin sealant is one of these alternatives. Besides being a validated scaffold and drug delivery system, it possesses excellent healing properties. This review covered the last 25 years of the literature and showed that the fibrin sealant is used in various clinical situations to promote the healing of different types of ulcers, especially chronic ones. These are mostly venous in origin and usually does not respond to conven...
Source: Journal of Venomous Animals and Toxins including Tropical Diseases - June 23, 2020 Category: Tropical Medicine Source Type: research

Recombinant antibodies against Iranian cobra venom as a new emerging therapy by phage display technology
Conclusion: Therefore, phage display is believed to have a good potential as an approach to the development of snake antivenom. (Source: Journal of Venomous Animals and Toxins including Tropical Diseases)
Source: Journal of Venomous Animals and Toxins including Tropical Diseases - June 20, 2020 Category: Tropical Medicine Source Type: research

Why does the number of dangerous species of scorpions increase? The particular case of the genus Leiurus Ehrenberg (Buthidae) in Africa
Abstract The aim of this contribution is to bring some precise information on the reasons why the number of noxious scorpion species is constantly growing. This fact is directly associated with the zoological research on the domains generally defined as systematics and taxonomy. The classification of any zoological group is in most cases a source of problem for most biologists not directly involved with this almost confidential aspect of the zoological research. Much information has been gathered and published over two centuries on the classification but it is remains poorly accessible and too technical for non-experts. Th...
Source: Journal of Venomous Animals and Toxins including Tropical Diseases - June 17, 2020 Category: Tropical Medicine Source Type: research

Mitochondrial activity disruption and local muscle damage induced in mice by Scolopendra polymorpha venom
Conclusion: Our results suggest a different biochemical composition of S. polymorpha venom, based on the different effects of four venom fractions on the cells tested, according to statistical evidence. Fractions F6 and F7 caused the most important alterations. (Source: Journal of Venomous Animals and Toxins including Tropical Diseases)
Source: Journal of Venomous Animals and Toxins including Tropical Diseases - May 29, 2020 Category: Tropical Medicine Source Type: research

Jingzhaotoxin-X, a gating modifier of Kv4.2 and Kv4.3 potassium channels purified from the venom of the Chinese tarantula Chilobrachys jingzhao
Conclusions: JZTX-X selectively suppresses Kv4.2 and Kv4.3 potassium channe l activity in a concentration- and voltage-dependent manner and causes long-lasting mechanical hyperalgesia. (Source: Journal of Venomous Animals and Toxins including Tropical Diseases)
Source: Journal of Venomous Animals and Toxins including Tropical Diseases - May 29, 2020 Category: Tropical Medicine Source Type: research

Extracellular vesicles in infectious diseases caused by protozoan parasites in buffaloes
Conclusions: EVs from buffaloes infected with Theileria spp. were successfully isolated and characterized. This is an advance in the knowledge of host-parasite relationship that contributes to the understanding of host immune response and theileriosis evasion mechanisms. These findings may pave the way for searching new EVs candidate-markers for a better production of safe biological products derived from buffaloes. (Source: Journal of Venomous Animals and Toxins including Tropical Diseases)
Source: Journal of Venomous Animals and Toxins including Tropical Diseases - May 29, 2020 Category: Tropical Medicine Source Type: research

Neutrophils activated by BJcuL, a C-type lectin isolated from Bothrops jararacussu venom, decrease the invasion potential of neuroblastoma SK-N-SH cells in vitro
ABSTRACT Background: Neuroblastoma is a pediatric tumor with a mortality rate of 40% in the most aggressive cases. Tumor microenvironment components as immune cells contribute to the tumor progression; thereby, the modulation of immune cells to a pro-inflammatory and antitumoral profile could potentialize the immunotherapy, a suggested approach for high-risk patients. Preview studies showed the antitumoral potential of BJcuL, a C- type lectin isolated from Bothrops jararacussu venom. It was able to induce immunomodulatory responses, promoting the rolling and adhesion of leukocytes and the activation of neutrophils. Methods...
Source: Journal of Venomous Animals and Toxins including Tropical Diseases - May 20, 2020 Category: Tropical Medicine Source Type: research

Neuroprotection and immunomodulation by dimethyl fumarate and a heterologous fibrin biopolymer after ventral root avulsion and reimplantation
Conclusion: The pharmacological benefit was further enhanced when associated with root reimplantation with FS, in which animals recovered at least 50% of motor function, showing the efficacy of employing multiple regenerative approaches following spinal cord root injury. (Source: Journal of Venomous Animals and Toxins including Tropical Diseases)
Source: Journal of Venomous Animals and Toxins including Tropical Diseases - May 20, 2020 Category: Tropical Medicine Source Type: research

Energetic supplementation for maintenance or development of Apis mellifera L. colonies
Conclusions: Therefore, sugar syrup is concluded to be the best artificial energetic food for use in the supplementation of honeybee colonies. (Source: Journal of Venomous Animals and Toxins including Tropical Diseases)
Source: Journal of Venomous Animals and Toxins including Tropical Diseases - May 16, 2020 Category: Tropical Medicine Source Type: research

Neutrophils activated by BJcuL, a C-type lectin isolated from Bothrops jararacussu venom, decrease the invasion potential of neuroblastoma SK-N-SH cells in vitro
ABSTRACT Background: Neuroblastoma is a pediatric tumor with a mortality rate of 40% in the most aggressive cases. Tumor microenvironment components as immune cells contribute to the tumor progression; thereby, the modulation of immune cells to a pro-inflammatory and antitumoral profile could potentialize the immunotherapy, a suggested approach for high-risk patients. Preview studies showed the antitumoral potential of BJcuL, a C- type lectin isolated from Bothrops jararacussu venom. It was able to induce immunomodulatory responses, promoting the rolling and adhesion of leukocytes and the activation of neutrophils. Methods...
Source: Journal of Venomous Animals and Toxins including Tropical Diseases - May 11, 2020 Category: Tropical Medicine Source Type: research

Isolation and structural identification of a new T1-conotoxin with unique disulfide connectivities derived from Conus bandanus
Conclusion: We identified the first T1-conotoxin, denominated Bn5a, isolated from C. bandanus venom. However, Bn5a conotoxin exhibited unique C1-C4/C2-C3 disulfide connectivity, unlike other T1-conotoxins (C1-C3/C2-C4). The structural and homological analyses herein have evidenced novel conotoxin Bn5a that may require further investigation. (Source: Journal of Venomous Animals and Toxins including Tropical Diseases)
Source: Journal of Venomous Animals and Toxins including Tropical Diseases - May 8, 2020 Category: Tropical Medicine Source Type: research

Gene sequence analysis of toxins from the spider Phoneutria nigriventer revealed an intronless feature
Conclusions: Despite a few nucleotide substitutions in the genomic regions encoding for the toxin precursors when compared with cDNA sequences, the results of the present work indicate that P. nigriventer toxins do not contain introns in their genes sequences. (Source: Journal of Venomous Animals and Toxins including Tropical Diseases)
Source: Journal of Venomous Animals and Toxins including Tropical Diseases - May 1, 2020 Category: Tropical Medicine Source Type: research

Scorpion stings in pregnancy: an analysis of outcomes in 66 envenomed pregnant patients in Iran
Conclusion: Envenomation significantly contributes to preterm birth. Moreover, the location of bites and the type of scorpion species have a decisive role in the pregnancy outcome of scorpion-envenomed pregnant women. (Source: Journal of Venomous Animals and Toxins including Tropical Diseases)
Source: Journal of Venomous Animals and Toxins including Tropical Diseases - May 1, 2020 Category: Tropical Medicine Source Type: research

Analgesic and side effects of intravenous recombinant Phα1β
Conclusion: Our data suggest that intravenous administration of recombinant Ph α 1 β may be feasible for drug-induced analgesia, without causing any severe side effects. (Source: Journal of Venomous Animals and Toxins including Tropical Diseases)
Source: Journal of Venomous Animals and Toxins including Tropical Diseases - April 17, 2020 Category: Tropical Medicine Source Type: research

Venomics and antivenomics of the poorly studied Brazil’s lancehead, Bothrops brazili (Hoge, 1954), from the Brazilian State of Pará
Conclusion: The proteomics outcome contribute to a deeper insight into the spectrum of toxins present in the venom of the Brazil ’ s lancehead, and rationalize the pathophysiology underlying this snake bite envenomings. The comparative qualitative and quantitative immunorecognition profile of the Brazilian pentabothropic antivenom toward the venom toxins of B. brazili and B. jararaca (the reference venom for assessing the bothropic antivenom's potency in Brazil), provides clues about the proper use of the Brazilian antibothropic polyvalent antivenom in the treatment of bites by the Brazil ’ s lancehead. (Source...
Source: Journal of Venomous Animals and Toxins including Tropical Diseases - April 17, 2020 Category: Tropical Medicine Source Type: research

Crotamine in Crotalus durissus: distribution according to subspecies and geographic origin, in captivity or nature
Conclusion: These differences between venom pools indicate the importance of using both, crotamine-positive and crotamine-negative venoms, to produce the antivenom. (Source: Journal of Venomous Animals and Toxins including Tropical Diseases)
Source: Journal of Venomous Animals and Toxins including Tropical Diseases - April 7, 2020 Category: Tropical Medicine Source Type: research

São Paulo School of Advanced Sciences on Vaccines: an overview
This report highlights some of the knowledge shared at the SPSASV. (Source: Journal of Venomous Animals and Toxins including Tropical Diseases)
Source: Journal of Venomous Animals and Toxins including Tropical Diseases - April 7, 2020 Category: Tropical Medicine Source Type: research

Biochemical characterization and cytotoxic effect of the skin secretion from the red-spotted Argentina frog Argenteohyla siemersi (Anura: Hylidae)
Conclusions: Results presented herein evidence the biochemical composition and biological effects of A. siemersi skin secretion and contribute to the knowledge on the defense mechanisms of casque-headed frogs. (Source: Journal of Venomous Animals and Toxins including Tropical Diseases)
Source: Journal of Venomous Animals and Toxins including Tropical Diseases - March 31, 2020 Category: Tropical Medicine Source Type: research

Identification and characterization of the first endogenous phospholipase A2 inhibitor from a non-venomous tropical snake, Boa constrictor (Serpentes: Boidae)
Conclusion: A functional sb γ PLI (BcNF) was described in the blood plasma of B. constrictor. BcNF displayed higher primary identity with sb γ PLIs from Viperidae than to sb γ PLIs from non-venomous species from Asia. The physiological role played by sb γ PLIs in non-venomous snake species remains to be understood. Further investigation is needed. (Source: Journal of Venomous Animals and Toxins including Tropical Diseases)
Source: Journal of Venomous Animals and Toxins including Tropical Diseases - March 13, 2020 Category: Tropical Medicine Source Type: research

Anticancer potential of nanogold conjugated toxin GNP-NN-32 from Naja naja venom
Conclusion: GNP-NN-32 showed an anticancer potential against human breast cancer cell lines. Analysis of detailed chemical characterization along with its cytotoxic property might help to perceive a new dimension of the anti-cancer potential of GNP-NN-32 that will enhance its biomedical function in near future. (Source: Journal of Venomous Animals and Toxins including Tropical Diseases)
Source: Journal of Venomous Animals and Toxins including Tropical Diseases - March 3, 2020 Category: Tropical Medicine Source Type: research

Antibiotic therapy for snakebite envenoming
Abstract There are numerous conflicting recommendations available on the use of antibiotics following snakebite. The present letter to the editor presents some recommendations based on recent studies, and aims to stimulate debate on this topic. (Source: Journal of Venomous Animals and Toxins including Tropical Diseases)
Source: Journal of Venomous Animals and Toxins including Tropical Diseases - February 4, 2020 Category: Tropical Medicine Source Type: research

Bufotenine, a tryptophan-derived alkaloid, suppresses the symptoms and increases the survival rate of rabies-infected mice: the development of a pharmacological approach for rabies treatment
Conclusions: Bufotenine is a promising candidate for drug development. After further chemical modification, it might be possible to dissociate minor side effects, increase efficiency, efficacy and pharmacokinetics, yielding a true anti-rabies drug. (Source: Journal of Venomous Animals and Toxins including Tropical Diseases)
Source: Journal of Venomous Animals and Toxins including Tropical Diseases - February 4, 2020 Category: Tropical Medicine Source Type: research

Proteomics and antivenom immunoprofiling of Russell’s viper (Daboia siamensis) venoms from Thailand and Indonesia
Conclusion: D. siamensis venoms from Thailand and Indonesia varied geographically in the protein subtypes and abundances. The venoms, nevertheless, shared conserved antigenicity that allowed effective immunorecognition by DsMAV-Thailand but not by SABU, consistent with the neutralization efficacy of the antivenoms. A specific, appropriate antivenom is needed in Indonesia to treat Russell ’ s viper envenomation. (Source: Journal of Venomous Animals and Toxins including Tropical Diseases)
Source: Journal of Venomous Animals and Toxins including Tropical Diseases - January 31, 2020 Category: Tropical Medicine Source Type: research

Effects of Mlx-8, a phospholipase A2 from Brazilian coralsnake Micrurus lemniscatus venom, on muscarinic acetylcholine receptors in rat hippocampus
Conclusion: The results of the present work show, for the first time, that muscarinic receptors are also affected by the Mlx-8 toxin, a m uscarinic ligand with phospholipase A2 characteristics, obtained from the venom of the Elapidae snake Micrurus lemniscatus, since this toxin was able to compete with muscarinic ligand [3H]QNB in hippocampus of rats. In addition, Mlx-8 also blocked the accumulation of total [3H]inositol phosphate ind uced by muscarinic agonist carbachol. Thus, Mlx-8 may be a new pharmacological tool for examining muscarinic cholinergic function. (Source: Journal of Venomous Animals and Toxins including Tropical Diseases)
Source: Journal of Venomous Animals and Toxins including Tropical Diseases - January 28, 2020 Category: Tropical Medicine Source Type: research

Heterologous expression and mutagenesis of recombinant Vespa affinis hyaluronidase protein (rVesA2)
Conclusion: The recombinant wild-type protein showed its maximal activity at pH 2, more acidic pH than that found in the crude venom. The glycosylation was predicted to be responsible for the pH optimum and thermal stability of the enzymes activity. (Source: Journal of Venomous Animals and Toxins including Tropical Diseases)
Source: Journal of Venomous Animals and Toxins including Tropical Diseases - December 6, 2019 Category: Tropical Medicine Source Type: research

Samsum ant venom modulates the immune response and redox status at the acute toxic dose in vivo
Conclusion: Stimulation of PMNs by SAV leads to upregulation of MHC-II, CD 80, and CD 86, which plays critical roles in antigen presentation and consequently proliferation of T-cells. Subcutaneous route was more efficient than intraperitoneal by elevating MHC-II, CD80 and CD86 expression, disturbing oxidative stability and increasing lipogram concentration. (Source: Journal of Venomous Animals and Toxins including Tropical Diseases)
Source: Journal of Venomous Animals and Toxins including Tropical Diseases - December 3, 2019 Category: Tropical Medicine Source Type: research

The WHO strategy for prevention and control of snakebite envenoming: a sub-Saharan Africa plan
Abstract Snakebite is a critical public health issue in tropical countries, particularly in Africa, where 20% of snakebites globally occur. In 2017, the WHO added snakebite envenoming to the category A of neglected tropical diseases. In 2019, thanks to broad institutional and international NGO support, including strong mobilization of African experts and governments, WHO launched a strategy for prevention and control of snakebite envenoming with more ambitious goals. In sub-Saharan Africa, accessibility of antivenoms and symptomatic, adjuvant or replacement therapy is a priority. Several antivenoms are available but their ...
Source: Journal of Venomous Animals and Toxins including Tropical Diseases - December 3, 2019 Category: Tropical Medicine Source Type: research

Unique heterologous fibrin biopolymer with hemostatic, adhesive, sealant, scaffold and drug delivery properties: a systematic review
Abstract Fibrin biopolymers, previously referred as “ fibrin glue ” or “ fibrin sealants ” , are natural biomaterials with diverse applications on health. They have hemostatic, adhesive, sealant, scaffold and drug delivery properties and have become widely used in medical and dental procedures. Historically, these biomaterials are produced from human fibrinogen and human or animal thrombin, and the possibility of transmission of infectious diseases by human blood is not ruled out. In the 1990s, to overcome this problem, a new heterologous biomaterial composed of a thrombin-like enzyme purified from ...
Source: Journal of Venomous Animals and Toxins including Tropical Diseases - November 12, 2019 Category: Tropical Medicine Source Type: research

Fibrin biopolymer as scaffold candidate to treat bone defects in rats
Conclusions: FBP proved to be an excellent scaffold candidate for bone repair therapies due to application ease and biocompatibility with synthetic calcium-based materials. The satisfactory results obtained by the association of FBP with MSCs may provide a more effective and less costly new approach for bone tissue engineering. (Source: Journal of Venomous Animals and Toxins including Tropical Diseases)
Source: Journal of Venomous Animals and Toxins including Tropical Diseases - November 5, 2019 Category: Tropical Medicine Source Type: research

Cytotoxic effects of Pseudocerastes persicus venom and its HPLC fractions on lung cancer cells
Conclusion: HPLC fraction 21 at low concentrations displayed promising anticancer properties with apoptosis induction in the lung cancer cells. This fraction may, therefore, be considered a promising candidate for further studies. (Source: Journal of Venomous Animals and Toxins including Tropical Diseases)
Source: Journal of Venomous Animals and Toxins including Tropical Diseases - September 17, 2019 Category: Tropical Medicine Source Type: research

Zika virus proteins at an atomic scale: how does structural biology help us to understand and develop vaccines and drugs against Zika virus infection?
Abstract In Brazil and in other tropical areas Zika virus infection was directly associated with clinical complications as microcephaly in newborn children whose mothers were infected during pregnancy and the Guillain-Barr é syndrome in adults. Recently, research has been focused on developing new vaccines and drug candidates against Zika virus infection since none of those are available. In order to contribute to vaccine and drug development efforts, it becomes important the understanding of the molecular basis of t he Zika virus recognition, infection and blockade. To this purpose, it is essential the structural d...
Source: Journal of Venomous Animals and Toxins including Tropical Diseases - August 30, 2019 Category: Tropical Medicine Source Type: research

Protein identification from the parotoid macrogland secretion of Duttaphrynus melanostictus
Conclusions: Proper sample preparation allowed the proteomic and de novo identification of different proteins in the D. melanostictus parotoid macrogland secretion. These results may increase the knowledge about the universe of molecules that compose amphibian skin secretion, as well as to understand their biological/physiological role in the granular gland. (Source: Journal of Venomous Animals and Toxins including Tropical Diseases)
Source: Journal of Venomous Animals and Toxins including Tropical Diseases - August 20, 2019 Category: Tropical Medicine Source Type: research

Antileishmanial activity and immunomodulatory effect of secosubamolide, a butanolide isolated from Nectandra oppositifolia (Lauraceae)
This study showed the antileishmanial activity of compounds 1 and 2 isolated from N. oppositifolia. Furthermore, compound 2 demonstrated an antileishmanial activity towards amastigotes associated to an immunomodulatory effect. (Source: Journal of Venomous Animals and Toxins including Tropical Diseases)
Source: Journal of Venomous Animals and Toxins including Tropical Diseases - August 20, 2019 Category: Tropical Medicine Source Type: research

Antinociceptive effect of PnTx4(5-5), a peptide from Phoneutria nigriventer spider venom, in rat models and the involvement of glutamatergic system
Conclusion: The antinociceptive effect for PnTx4(5-5) was demonstrated against different rat pain models, i.e. induced by PGE2, carrageenan or glutamate. We suggest that the antinociceptive effect of PnTx4(5-5) may be related to an inhibitory activity on the glutamatergic system. (Source: Journal of Venomous Animals and Toxins including Tropical Diseases)
Source: Journal of Venomous Animals and Toxins including Tropical Diseases - August 13, 2019 Category: Tropical Medicine Source Type: research

Ethnomedicinal plants used for snakebite treatments in Ethiopia: a comprehensive overview
Abstract Traditional medicine plays an important role in the daily lives of people living in rural parts of Ethiopia. Despite the fact that Ethiopia has a long history of using traditional medicinal plants as an alternative medicine source, there is no checklist compiling these plants used for snakebite treatment. This review collected and compiled available knowledge on and practical usage of such plants in the country. A literature review on medicinal plants used to treat snakebites was conducted from 67 journal articles, PhD dissertation and MSc theses available online. Data that summarize scientific and folk names, adm...
Source: Journal of Venomous Animals and Toxins including Tropical Diseases - August 6, 2019 Category: Tropical Medicine Source Type: research

BthTX-I from Bothrops jararacussu induces apoptosis in human breast cancer cell lines and decreases cancer stem cell subpopulation
Conclusions: BthTX-I induces apoptosis and autophagy in all breast cancer cell lines tested and also reduces CSC s subpopulation, which makes it a promising therapeutic alternative for breast cancer. (Source: Journal of Venomous Animals and Toxins including Tropical Diseases)
Source: Journal of Venomous Animals and Toxins including Tropical Diseases - July 30, 2019 Category: Tropical Medicine Source Type: research

Is the cat an important reservoir host for visceral leishmaniasis? A systematic review with meta-analysis
Abstract In recent years feline leishmanial infections (FLI) have been studied more than ever before in various parts of the world. However, evidence-based knowledge on FLI has remained unavailable. The main objectives of this study were to investigate the status of felines infected by Leishmania spp. worldwide. Data were extracted from 10 available databases over the period of 1982 to 2017. Overall, 78 articles fulfilled the inclusion criteria and were used for data extraction in this systematic review. The overall FLI prevalence by both serological and molecular methods was estimated at 10% (95% CI: 8%-14%). In Italy, bo...
Source: Journal of Venomous Animals and Toxins including Tropical Diseases - June 11, 2019 Category: Tropical Medicine Source Type: research

Using prodigiosin against some gram-positive and gram-negative bacteria and Trypanosoma cruzi
Conclusions: This work demonstrates the potential usefulness of prodigiosin against some gram-positive and gram-negative bacteria and Trypanosoma cruzi although further studie s must be done in order to assess its value as a candidate molecule. (Source: Journal of Venomous Animals and Toxins including Tropical Diseases)
Source: Journal of Venomous Animals and Toxins including Tropical Diseases - June 3, 2019 Category: Tropical Medicine Source Type: research

Spider venom peptides as potential drug candidates due to their anticancer and antinociceptive activities
Abstract Spider venoms are known to contain proteins and polypeptides that perform various functions including antimicrobial, neurotoxic, analgesic, cytotoxic, necrotic, and hemagglutinic activities. Currently, several classes of natural molecules from spider venoms are potential sources of chemotherapeutics against tumor cells. Some of the spider peptide toxins produce lethal effects on tumor cells by regulating the cell cycle, activating caspase pathway or inactivating mitochondria. Some of them also target the various types of ion channels (including voltage-gated calcium channels, voltage-gated sodium channels, and aci...
Source: Journal of Venomous Animals and Toxins including Tropical Diseases - June 3, 2019 Category: Tropical Medicine Source Type: research

Divergences between the Brazilian national information systems for recording deaths from venomous animals
Conclusion: Comparative analysis of these databases permits identification of important differences between profiles presented by thes e systems, which have equal relevance for Brazil as a whole and for its regions. These differences may influence the construction of various scenarios. (Source: Journal of Venomous Animals and Toxins including Tropical Diseases)
Source: Journal of Venomous Animals and Toxins including Tropical Diseases - May 21, 2019 Category: Tropical Medicine Source Type: research

Neuroprotective properties of RT10, a fraction isolated from Parawixia bistriata spider venom, against excitotoxicity injury in neuron-glia cultures
Conclusion: RT10 fraction exhibits neuroprotective effects against L-Glu excitotoxicity in neuron-glia c ultured in vitro. (Source: Journal of Venomous Animals and Toxins including Tropical Diseases)
Source: Journal of Venomous Animals and Toxins including Tropical Diseases - May 17, 2019 Category: Tropical Medicine Source Type: research

Traceability of animal protein byproducts in ruminants by multivariate analysis of isotope ratio mass spectrometry to prevent transmission of prion diseases
Conclusions: IRMS is an accurate and sensitive technique for tracing the feeding profile of ruminants through blood analysis, thus enabling investigation of ABP use. (Source: Journal of Venomous Animals and Toxins including Tropical Diseases)
Source: Journal of Venomous Animals and Toxins including Tropical Diseases - May 13, 2019 Category: Tropical Medicine Source Type: research

Effects of copaiba oil on dermonecrosis induced by Loxosceles intermedia venom
Conclusions: We conclude that copaiba oil may interfere in the healing process and thus propose it as a possible topical treatment for cutaneous lesions induced by L. intermedia venom. (Source: Journal of Venomous Animals and Toxins including Tropical Diseases)
Source: Journal of Venomous Animals and Toxins including Tropical Diseases - April 25, 2019 Category: Tropical Medicine Source Type: research

Assessment of neuropharmacological potential of low molecular weight components extracted from Rhinella schneideri toad poison
Abstract Background: Studies on toad poison are relevant since they are considered a good source of toxins that act on different biological systems. Among the molecules found in the toad poison, it can be highlighted the cardiotonic heterosides, which have a known mechanism that inhibit Na+/K+-ATPase enzyme. However, these poisons have many other molecules that may have important biological actions. Therefore, this work evaluated the action of the low molecular weight components from Rhinella schneideri toad poison on Na+/K+-ATPase and their anticonvulsive and / or neurotoxic effects, in order to detect molecules with acti...
Source: Journal of Venomous Animals and Toxins including Tropical Diseases - April 18, 2019 Category: Tropical Medicine Source Type: research

Proteome of fraction from Tityus serrulatus venom reveals new enzymes and toxins
Conclusions: The combination of a proteomic and biochemical characterization strategies leads us to identify new components in the T. serrulatus scorpion venom. The proteome of venom ´s fraction can provide valuable direction in the obtainment of components in their native forms in order to perform a preliminary characterization and, consequently, to promote advances in biological discoveries in toxinology. (Source: Journal of Venomous Animals and Toxins including Tropical Diseases)
Source: Journal of Venomous Animals and Toxins including Tropical Diseases - April 18, 2019 Category: Tropical Medicine Source Type: research

Subproteome of Lachesis muta rhombeata venom and preliminary studies on LmrSP-4, a novel snake venom serine proteinase
Conclusions: Novel serine proteinase isoforms and a lectin were identified in LmrV. Additionally, a kallikrein-like serine proteinase that might be useful as molecular tool for investigating bradykinin -involving process was isolated and partially characterized. (Source: Journal of Venomous Animals and Toxins including Tropical Diseases)
Source: Journal of Venomous Animals and Toxins including Tropical Diseases - April 16, 2019 Category: Tropical Medicine Source Type: research

Scorpion toxins targeting Kv1.3 channels: insights into immunosuppression
Abstract Scorpion venoms are natural sources of molecules that have, in addition to their toxic function, potential therapeutic applications. In this source the neurotoxins can be found especially those that act on potassium channels. Potassium channels are responsible for maintaining the membrane potential in the excitable cells, especially the voltage-dependent potassium channels (Kv), including Kv1.3 channels. These channels (Kv1.3) are expressed by various types of tissues and cells, being part of several physiological processes. However, the major studies of Kv1.3 are performed on T cells due its importance on autoimm...
Source: Journal of Venomous Animals and Toxins including Tropical Diseases - April 16, 2019 Category: Tropical Medicine Source Type: research

Functional and biological insights of rCollinein-1, a recombinant serine protease from Crotalus durissus collilineatus
Conclusion: These results suggest that the recombinant enzyme has no potential as a thrombolytic agent but can be applied in the prevention of thrombus formation in some pathological processes and as molecular tools in studies related to hemostasis. (Source: Journal of Venomous Animals and Toxins including Tropical Diseases)
Source: Journal of Venomous Animals and Toxins including Tropical Diseases - April 8, 2019 Category: Tropical Medicine Source Type: research

BjussuLAAO-II induces cytotoxicity and alters DNA methylation of cell-cycle genes in monocultured/co-cultured HepG2 cells
Abstract Background: The use of animal venoms and their toxins as material sources for biotechnological applications has received much attention from the pharmaceutical industry. L-amino acid oxidases from snake venoms (SV-LAAOs) have demonstrated innumerous biological effects and pharmacological potential against different cancer types. Hepatocellular carcinoma has increased worldwide, and the aberrant DNA methylation of liver cells is a common mechanism to promote hepatic tumorigenesis. Moreover, tumor microenvironment plays a major role in neoplastic transformation. To elucidate the molecular mechanisms responsible for ...
Source: Journal of Venomous Animals and Toxins including Tropical Diseases - March 11, 2019 Category: Tropical Medicine Source Type: research