A method for detecting tumor cells derived from colorectal cancer by targeting cell surface glycosylation with affinity capillary electrophoresis
Publication date: Available online 30 January 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Tetsushi Yamamoto, Kanta Sato, Shinpei Wakahara, Kuniko Mitamura, Atsushi TagaAbstractCirculating tumor cells (CTCs) are involved in metastasis; thus, one of the most important approaches for identifying metastatic cancer is to detect CTCs in blood. In the present study, we examined whether directly analyzing cells with capillary electrophoresis (CE) could distinguish cancer cells from normal cells, based on differences in cell surface glycosylation. We compared human colorectal cancer (CRC) cell lines to a...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 31, 2020 Category: Drugs & Pharmacology Source Type: research

Aspects of Matrix and Analyte Effects in Clinical Pharmacokinetic Sample Analyses using LC-ESI/MS/MS – Two Case Examples
Publication date: Available online 30 January 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Guohua An, Thanh Bach, Inas Abdallah, Demet NalbantAbstractThe increasing focus on high throughput sample analysis has led to the common practice of using simplest sample preparation method possible (i.e. protein precipitation) and shortest sample run-time possible. This means that there will be two aspects of compromise: the first compromise is made between sample cleanliness and sample preparation speed since protein precipitation does not provide very clean final extract; the second compromise is made be...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 30, 2020 Category: Drugs & Pharmacology Source Type: research

High Performance Liquid Chromatography-Electrospray Ionization Mass Spectrometric (LC-ESI-MS) Methodology for Analysis of Amino Acid Energy Substrates in Microwave-Fixed Microdissected Brain Tissue
Publication date: Available online 28 January 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Khaggeswar Bhehmanapally, Mostafa M.H. Ibrahim, karen P.B. BriskiAbstractHypoglycemia deprives the brain of its primary energy source glucose. Reductions in whole-brain amino acid energy substrate levels suggest that these non-glucose fuels may be metabolized during glucose shortage. Recurring hypoglycemia can cause mal-adaptive impairment of glucose counter-regulation; yet, it is unclear if amplified reliance upon alternative metabolic substrates impedes detection of continuing neuro-glucopenia. This resea...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 29, 2020 Category: Drugs & Pharmacology Source Type: research

Preparation and application of teicoplanin functionalized polymeric monolith for enantioseparation of chiral drugs
Publication date: Available online 27 January 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Rongying Luo, Hai Han, Jia Liu, Dongsheng Xu, Qiqin Wang, Salvatore Fanali, Zhengjin JiangAbstractA novel chiral stationary phase (CSP), based on a monolithic organic polymer chemically modified with teicoplanin, was fabricated within a 100 μm I.D. fused silica capillary. The teicoplanin was firstly derivatized with 2-isocyanatoethyl methacrylate (ICNEML) and then thermally co-polymerized with the crosslinker ethylene dimethacrylate (EDMA) in presence of porogens (methanol and dimethylsulfoxide). ...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 28, 2020 Category: Drugs & Pharmacology Source Type: research

Analytical Artifacts in characterization of recombinant monoclonal antibody therapeutics
Publication date: Available online 25 January 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Dongdong Wang, Christine Nowak, Bruce Mason, Amit Katiyar, Hongcheng LiuAbstractA battery of analytical methods is used to analyze recombinant monoclonal antibodies for lot release to ensure consistent product quality, safety, and efficacy. Additionally, state-of-the-art analytical methods have been used to thoroughly characterize various post-translational modifications and degradation pathways of those molecules. Scientifically sound and robust analytical methods are essential to providing reliable result...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 26, 2020 Category: Drugs & Pharmacology Source Type: research

A new validated HPLC-UV method for therapeutic monitoring of daptomycin in comparison with reference Mass Spectrometry
In conclusion, the present method demonstrated to be reliable and suitable for daptomycin TDM in clinical routine. (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - January 26, 2020 Category: Drugs & Pharmacology Source Type: research

Automated middle-up approach for the characterization of biotherapeutic products by combining on-line hinge-specific digestion with RPLC-HRMS analysis
This study describes, for the first time, the development of an automated liquid chromatography-mass spectrometry (LC-MS) workflow comprised of an immobilized IdeS-HPLC column for on-line sample digestion, followed by a reversed-phase liquid chromatography (RPLC) for protein subunit separation, and a high-resolution MS for molecular weight analysis. A proof of concept study was described here for the characterization of a therapeutic mAb and a bsAb. For the mAb, this automated workflow enabled rapid characterization of post-translational modifications (PTMs) such as N-glycosylation, glycation and N-terminal lysine. For the...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 26, 2020 Category: Drugs & Pharmacology Source Type: research

Synthetic NRG-1 functionalized DNA nanospindels towards HER2/neu targets for in vitro anti-cancer activity assessment against breast cancer MCF-7 cells
Publication date: Available online 25 January 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Mirza Muhammad Faran Ashraf Baig, Wing-Fu Lai, Reyaj Mikrani, Mehreen Jabeen, Muhammad Naveed, Muhammad Abbas, Muhammad Asim Farooq, Anam Ahsan, Said Abasse Kassim, Ghulam Jilany Khan, Muhammad Tayyab AnsariAbstractDNA based nano-carriers synthesized from short circular scaffolds (circular DNA nanotechnology) attains stiffer topology for ligand functionalization (neuregulin-1/NRG-1 ligand) and biological applications (targeted drug delivery). Daunorubicin (DR) is a hydrophobic chemical that requires robust ...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 26, 2020 Category: Drugs & Pharmacology Source Type: research

Discovery and validation of peptide biomarkers for discrimination of Dendrobium species by label-free proteomics and chemometrics
This study committed to discover specific peptides biomarkers for the authentication of D. officinale from the other two Dendrobium species using label-free proteomics by nanoLC LTQ Orbitrap mass spectrometry. Multivariate statistical analysis was applied to visualize the difference between the three Dendrobium species. As a result, 29 peptides among a total of 343 measurable peptides were selected to be potential biomarkers for the classification of these Dendrobium species. The validation of the representative peptide biomarkers was carried out by the synthesized peptides and 3 peptide biomarkers were found significant f...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 25, 2020 Category: Drugs & Pharmacology Source Type: research

Plasma mevalonic acid exposure as a pharmacodynamic biomarker of fluvastatin/atorvastatin in healthy volunteers
In conclusion, the data show that the plasma exposure of MVL represents a reliable pharmacodynamic parameter for PK-PD (pharmacokinetic-pharmacodynamic) studies of fluvastatin in multiple doses and atorvastatin in a single dose. (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - January 25, 2020 Category: Drugs & Pharmacology Source Type: research

Opportunities and obstacles for microsampling techniques in bioanalysis: Special focus on DBS and VAMS
Publication date: Available online 25 January 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Vaishali Londhe, Madhura RajadhyakshaAbstractMicrosampling, a reduced volume (
Source: Journal of Pharmaceutical and Biomedical Analysis - January 25, 2020 Category: Drugs & Pharmacology Source Type: research

Combination of intact, middle-up and bottom-up levels to characterize 7 therapeutic monoclonal antibodies by capillary electrophoresis – mass spectrometry
In this study, 7 worldwide health authorities approved mAbs have been analyzed to get information about their charge heterogeneity, the identification of post translational modifications (PTMs), their location and relative quantitation. Intact mAbs isoforms have been partially separated in less than 12 minutes and enabled to have a global illustration of mAbs heterogeneity and high masses PTMs characterization notably major N-glycosylation forms. Particularly, 2X-glycosylated and 1X-glycosylated forms have been partially separated. To deepen characterize PTMs carried by the backbone structure, advanced investigation...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 25, 2020 Category: Drugs & Pharmacology Source Type: research

Evaluation of a sensitive GC-MS method to detect polysorbate 80 in vaccine preparation
Publication date: Available online 23 January 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Niraj Shende, Asha Mallya (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - January 25, 2020 Category: Drugs & Pharmacology Source Type: research

Multivariate Quantitative Analysis of Quality Trend Based on Non-volatile Characteristic Components in different Panax notoginseng samples using HPLC
Publication date: Available online 23 January 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Chao Li, Yunhua Qin, Qianxu Yang, Junheng You, Zhihua Liu, Jingmei Han, E’xian Li, Chengming ZhangAbstractIn order to identify real and fake Panax notoginseng samples, the high performance liquid chromatography (HPLC) was used to analyze P. notoginseng samples of non-volatile characteristic components in P. notoginseng powder samples with 10%, 30%, 50% ratio, combined with principal component analysis - mahalanobis distance (PCA-MD). The results showed that: (1) The PCA analysis showed that mahalanobi...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 25, 2020 Category: Drugs & Pharmacology Source Type: research

Editorial Board
Publication date: 20 February 2020Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 180Author(s): (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - January 25, 2020 Category: Drugs & Pharmacology Source Type: research

Development of an ADP-Ribosylation Assay for residual toxicity in C. difficile Binary Toxin CDTa using Automated Capillary Western Blot
Publication date: Available online 23 January 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Richard R. Rustandi, Melissa HammAbstractCDTa, an actin ADP-ribosylation transferase, is a binary toxin produced by the bacterium Clostridium difficile which is commonly associated with the hypervirulent strain present in Clostridium difficile infections. The mutated form of CDTa, 4mCDTa, is one of the components in the tetravalent Clostridium difficile vaccine in which the residual toxicity of the ADP-ribosylation activity needs to be monitored for safety reasons. There are several ADP- ribosylation activi...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 23, 2020 Category: Drugs & Pharmacology Source Type: research

Simultaneous determination of carvacrol and thymol in bee pollen by using a simple and efficient solvent extraction method and gas chromatography-mass spectrometry
Publication date: Available online 23 January 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Ana M. Ares, María J. Nozal, José L. Bernal, José BernalAbstractA novel method is proposed to determine residues of carvacrol and thymol in bee pollen by means of gas chromatography coupled to mass spectrometry. This is an efficient and simple sample treatment (with average analyte recoveries between 90% and 104%) involving solvent extraction with hexane followed by evaporation. There is no need for any additional clean-up step, as the matrix did not affect determination of mass spectro...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 23, 2020 Category: Drugs & Pharmacology Source Type: research

Simultaneous quantification of thirty polyphenols in blackthorn flowers and dry extracts prepared thereof: HPLC-PDA method development and validation for quality control
Publication date: Available online 21 January 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Anna Marchelak, Monika Anna Olszewska, Aleksandra OwczarekAbstractThe paper presents development and validation of a RP-HPLC-PDA method for quantification of 30 phenolic constituents of the blackthorn (Prunus spinosa L.) flower, a traditional European herbal medicine with a unique and complex composition. The target analytes were selected from over 50 active compounds present in the investigated plant material, and their separation was optimized on a C18 Ascentis Express fused-core column (2.7 μm, 150 mm...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 22, 2020 Category: Drugs & Pharmacology Source Type: research

Development and Validation of a New IgE-Tolerant ELISA Method for Quantifying Serum Concentration of Omalizumab
ConclusionsIn conclusion, by dissociating IgE-omalizumab complex, the authors proposed for the first time a new validated IgE-tolerant ELISA assay to determine omalizumab concentration in serum samples.Graphical abstract (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - January 22, 2020 Category: Drugs & Pharmacology Source Type: research

Headspace gas chromatographic method for antimicrobial screening: minimum inhibitory concentration determination
Publication date: Available online 21 January 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Menghui Li, Chunyun Zhang, Guilin Chen, Lutfun Nahar, Satyajit D. Sarker, Mingquan GuoAbstractThe headspace gas chromatographic method has been widely used to detect volatile metabolites to reflect the growth state of microorganisms, however, it has never been used for the determination of the minimum inhibitory concentration in antibacterial drugs. This paper reports a new method for evaluating the antimicrobial activity of drugs by measuring the amount of CO2 produced by bacterial metabolism after treatme...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 22, 2020 Category: Drugs & Pharmacology Source Type: research

Characterization of therapeutic oligonucleotides by liquid chromatography
Publication date: Available online 20 January 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Alexandre Goyon, Peter Yehl, Kelly ZhangABSTRACTMarketed therapies in the pharmaceutical landscape are rapidly evolving and getting more diverse. Small molecule medicines have dominated in the past while antibodies have grown dramatically in recent years. However, the failure of traditional small and large molecules in accessing certain targets has led to increased R&D efforts to develop alternative modalities. Therapeutic oligonucleotides (ONs) can accurately be directed against their ribonucleic acid ...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 20, 2020 Category: Drugs & Pharmacology Source Type: research

HPLC-MS/MS method for the simultaneous quantification of dolutegravir, elvitegravir, rilpivirine, darunavir, ritonavir, raltegravir and raltegravir-β-D-glucuronide in human plasma
Publication date: Available online 18 January 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Yi Zheng, Radia Aboura, Sana Boujaafar, Gabrielle Lui, Déborah Hirt, Naïm Bouazza, Frantz Foissac, Jean-Marc Treluyer, Sihem Benaboud, Inès GanaAbstractTherapeutic drug monitoring (TDM) is essential in the optimization of antiretroviral (ARV) treatments. In this work, we describe a new method for the simultaneous quantification of six molecules: the three novel ARV agents dolutegravir (DTG), elvitegravir (ELV) and rilpivirine (RPV), the first integrase inhibitor raltegravir (RAL) and its ...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 19, 2020 Category: Drugs & Pharmacology Source Type: research

Development, validation and application of a new HPLC-DAD method for simultaneous quantification of apixaban, dabigatran, edoxaban and rivaroxaban in human plasma
Publication date: Available online 14 January 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Filipa Gouveia, Joana Bicker, Joana Santos, Marília Rocha, Gilberto Alves, Amílcar Falcão, Ana FortunaAbstractDirect oral anticoagulants (DOACs) have been commonly used for the treatment of venous thromboembolism and for the prevention of stroke in patients with atrial fibrillation. Despite not being initially recommended, monitoring DOACs plasma concentrations is now recognized as essential in emergency situations and in special populations. Moreover, the inter-individual variability f...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 16, 2020 Category: Drugs & Pharmacology Source Type: research

Spectrochemical identification of kanamycin resistance genes in artificial microbial communities using Clover-assay
Publication date: Available online 15 January 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Naifu Jin, Camilo L.M. Morais, Francis L. Martin, Dayi ZhangAbstractPersistent abuse and overuse of antibiotics induces a widespread bloom of antibiotic resistance genes (ARGs) and the emergence of superbugs. A method designed to rapidly quantify ARGs in real-world scenarios is urgently needed. Here, we present an orthogonal test of heavy water and kanamycin exposure, namely, a “clover-assay”, to reveal the capability of state-of-the-art Raman microspectroscopy to identify ARGs within microbial ...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 16, 2020 Category: Drugs & Pharmacology Source Type: research

Chemical profiling of Callicarpa nudiflora and its effective compounds identification by compound-target network analysis
This study provided an opportunity to deepen the understanding of the chemical composition and the potential anti-inflammatory mechanism of C. nudiflora. (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - January 16, 2020 Category: Drugs & Pharmacology Source Type: research

Characterization of bromelain indicates a molar excess of inhibitor vs. enzyme molecules, a Jacalin-like lectin and Maillard reaction products
We described two methods to separate the inhibitor fraction from the enzyme fraction, RP-HPLC and size exclusion chromatography. A pineapple derived Jacalin-like-lectin, herein called ‘Anlec’, was identified and quantified by RP-HPLC-MS in bromelain and its content was determined to be 5%, related to all proteins in bromelain. Anlec binds specifically to mannose-containing glycans and is discussed in literature to possess anti-HIV medical potential. Bromelain could therefore be a possible and economic source for the production of Anlec. An isolation strategy of Anlec from bromelain, in high purity, is shown in ...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 16, 2020 Category: Drugs & Pharmacology Source Type: research

Development of Homogeneous Plasmonic Potency Assay Using Gold Nanoparticle Immunocomplexes
In this study, we demonstrated that the AuNP-based homogeneous plasmonic immunoassay (HPI) generated comparable potency values of a therapeutic mAb to a conventional binding ELISA in relatively shorter assay time and steps. Binding HPI can be potentially implemented as a potency assay for therapeutic mAbs in quality control laboratories.Graphical abstract (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - January 12, 2020 Category: Drugs & Pharmacology Source Type: research

Simultaneous determination of fluoxetine, venlafaxine, vortioxetine and their active metabolites in human plasma by LC-MS/MS using one-step sample preparation procedure
Publication date: Available online 11 January 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Martin Kertys, Michaela Krivosova, Igor Ondrejka, Igor Hrtanek, Ingrid Tonhajzerova, Juraj MokryAbstractThe aim of antidepressant therapy is to induce remission and prevent relapses of major depressive disorder with minimum adverse effects during the treatment. Due to high variability in metabolism, therapeutic drug monitoring is recommended as a useful tool for individualisation of the therapy. For this purpose, we have developed simple and sensitive ultra-high performance liquid chromatography-tandem mass...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 12, 2020 Category: Drugs & Pharmacology Source Type: research

Spectroscopic characterisation of a series of Salmonella Typhi Vi-diphtheria toxoid glycoconjugate antigens differing in polysaccharide-protein ratio
We describe here the further spectroscopic characterisation of this series of glycoconjugate immunogen bulks using NMR spectroscopy, circular dichroism and absorption spectroscopy. We have used several mathematical approaches to extract information from the spectroscopic data not previously applied to glycoconjugates. These complementary approaches provide information on (i) the integrity of the carrier protein, (ii) consistency between batches of vaccine components, (iii) the polysaccharide: protein ratio (iv) the O-acetylation of the Vi in the conjugate (v) the stability of the O-acetylation of the Vi, and (vi) the prese...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 10, 2020 Category: Drugs & Pharmacology Source Type: research

Simultaneous determination of a promising anti-brain tumor agent CAT3 and its two major metabolites in mouse plasma and brain by a LC-MS/MS method
Publication date: Available online 9 January 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Shengyu Zhao, Ru-Bing Wang, Jie Bai, Xiaoqing Fan, Minwan Hu, Baolian Wang, Jinping Hu, Yan LiAbstractA rapid and reproducible method with high selectivity was developed for simultaneous determination of a promising anti-brain tumor agent CAT3 and its two metabolites PF403 and GLU-PF403 in mouse plasma and brain. An economic deproteinization with septuple acetonitrile (v/v) was applied to pretreat the samples in this study. All analytes were well retained and separated on a CAPCELL CORE PC (2.7 μm,...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 10, 2020 Category: Drugs & Pharmacology Source Type: research

Stability of Sodium Ascorbyl Phosphate in the Water-Glycerol System
Publication date: Available online 9 January 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Xiaohui Dong, Ting Zhang, Hongyuan Wei, Leping DangAbstractSodium ascorbyl phosphate is a hydrophilic derivative of ascorbic acid with better stability compared to the parent compound. However, sodium ascorbyl phosphate is not as stable in solution as it is in the solid state, and it has been found to degrade, with accompanying discoloration, under the influence of different conditions. Here, the degradation mechanism of sodium ascorbyl phosphate in the water-glycerol system was revealed and the thermal degr...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 10, 2020 Category: Drugs & Pharmacology Source Type: research

The Simultaneous Quantification of Phytosterols And Tocopherols in Liposomal Formulations Using Validated Atmospheric Pressure Chemical Ionization- Liquid Chromatography –Tandem Mass Spectrometry
Publication date: Available online 9 January 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Asmita Poudel, George Gachumi, Ildiko Badea, Zafer Dallal Bashi, Anas El-AneedAbstractA novel liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed and validated to simultaneously quantify phytosterols (brassicasterol, campesterol, stigmasterol and β-sitosterol) and tocopherols (alpha, beta, gamma and delta) entrapped in the lipid bilayer of a liposomal formulation. Atmospheric pressure chemical ionization (APCI) was employed due to the enhanced ionization of phytosterols and to...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 10, 2020 Category: Drugs & Pharmacology Source Type: research

Simplification of affinity macroporous monolith microfluidic column synthesis and its ability for protein separation
Publication date: Available online 8 January 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Ashish Khaparde, Kishore K.R. TetalaAbstractA generic multi-component approach was designed to perform simultaneous in situ polymerization and ligand immobilization to develop affinity porous polymer based chromatography resin in a facile mode. This strategy exploits the regioselective ring opening reaction between epoxy group of monomer and native functional group of ligand (i.e. amine) under aqueous condition (pH 9.7).As a proof-of-concept, reaction of iminodiacetic acid (IDA) with allyl glycidyl ether (AG...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 9, 2020 Category: Drugs & Pharmacology Source Type: research

On-Flow LC-MS/MS Method for Screening of Xanthine Oxidase Inhibitors
Publication date: Available online 7 January 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Marili V.N. Rodrigues, Caio Rodrigues-Silva, Sinésio Boaventura, Adriana S.S. Oliveira, Susanne Rath, Quezia B. CassAbstractThe screening of compounds is the initial step in research for the development of new drugs. For this reason, the availability of fast and reliable tools for the screening of a large number of compounds becomes essential. Among the therapeutic targets, the enzyme xanthine oxidase (XO) is of great interest for its importance as a biological source of superoxide radicals, which con...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 9, 2020 Category: Drugs & Pharmacology Source Type: research

Characterization of bromelain indicates a molar excess of inhibitor vs enzyme molecules, a Jacalin-like lectin and Maillard reaction products
We described two methods to separate the inhibitor fraction from the enzyme fraction, RP-HPLC and size exclusion chromatography. A pineapple derived Jacalin-like-lectin, herein called ‘Anlec’, was identified and quantified by RP-HPLC-MS in bromelain and its content was determined to be 5 %, related to all proteins in bromelain. Anlec binds specifically to mannose-containing glycans and is discussed in literature to possess anti-HIV medical potential. Bromelain could therefore be a possible and economic source for the production of Anlec. An isolation strategy of Anlec from bromelain, in high purity, is sho...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 9, 2020 Category: Drugs & Pharmacology Source Type: research

LC-MS/MS assay coupled with carboxylic acid magnetic bead affinity capture to quantitatively measure cationic host defense peptides (HDPs) in complex matrices with application to preclinical pharmacokinetic studies
Publication date: Available online 2 January 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Maura J. O’Neill, King Chan, Jesse M. Jaynes, Zachary Knotts, Xia Xu, Abisola Abisoye-Ogunniyan, Theresa Guerin, Jerome Schlomer, Dandan Li, Jeffrey W. Cary, Kanniah Rajasekaran, Clayton Yates, Serguei Kozlov, Thorkell Andresson, Udo RudloffAbstractSynthetic host defense peptides (HDP) are a new class of promising therapeutic agents with potential application in a variety of diseases. RP-182 is a 10mer synthetic HDP design, which selectively reduces M2-like tumor associated macrophages via engagement w...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 7, 2020 Category: Drugs & Pharmacology Source Type: research

Assessment method for deamidation in proteins using carboxylic acid derivatization-liquid chromatography-tandem mass spectrometry
Publication date: Available online 3 January 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Shimba Kawasue, Yohei Sakaguchi, Reiko Koga, Hideyuki Yoshida, Hitoshi NohtaAbstractAn analytical method for the degree of protein deamidation has been developed by using carboxy group derivatization and liquid chromatography-tandem mass spectrometry (LC-MS/MS). The fragment peptides (LGEYGFQNALIVR and YNGVFQECCQAEDK) obtained by digesting bovine serum albumin (BSA) with trypsin and their asparagine deamidated peptides (LGEYGFQDALIVR and YDGVFQECCQAEDK) were selected as model peptides, and their carboxy grou...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 7, 2020 Category: Drugs & Pharmacology Source Type: research

Chemical Analysis of Saffron by HPLC based Crocetin Estimation
Publication date: Available online 3 January 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Chilakala Nagarjuna Reddy, Sandip B. Bharate, Ram A. Vishwakarma, Sonali S. BharateAbstractSaffron is one of the most expensive and valuable spice having tremendous commercial value in food industry and thus its quality-check is of utmost importance. Crocins are unique group of extremely hydrophilic apocarotenoids which are main components of saffron. Crocetin is an aglycone of crocins which do occur naturally in saffron, and is produced in biological system as a bioactive-metabolite via hydrolytic cleavage ...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 7, 2020 Category: Drugs & Pharmacology Source Type: research

A sensitive nanocomposite design via carbon nanotube and silver nanoparticles: Selective probing of Emedastine Difumarate
In this study, a novel and sensitive nanocomposite of carboxylate-functionalized multiwalled carbon nanotube (COOH-fMWCNT) and silver nanoparticles (AgNPs) was fabricated and used to modify a glassy carbon electrode (GCE) by a simple drop casting method. Modified electrode was then applied for selective determination of emedastine difumarate (EDD). The COOH-fMWCNT/AgNPs nanocomposite was characterized by electrochemical impedance spectroscopy (EIS), scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX) and cyclic voltammetry (CV). EDD showed two oxidation peaks at 0.634 and 1.2 V on the GCE ...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 7, 2020 Category: Drugs & Pharmacology Source Type: research

Development and investigation of a QuEChERS-based method for determination of phthalate metabolites in human milk
Publication date: 20 March 2020Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 181Author(s): Jinyoung An, Ye-Yeong Kim, Hyun-Deok Cho, Junhee Kim, Ji-Youn Lee, Yeoreum Lee, Eunji Jo, Jaeick Lee, Sangwon Cha, Sang Beom HanAbstractPhthalates are commonly used as plasticizers and are known as risk factors toward several conditions such as cancer, birth defects, and endocrine disruption. Biomonitoring of phthalates is necessary to assess the potentially harmful effects of long-term exposure. In this work, we have developed a novel QuEChERS method to determine eight phthalate metabolites—mono-(3-carboxyp...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 7, 2020 Category: Drugs & Pharmacology Source Type: research

Development and validation of a UPLC–MS/MS method for simultaneous determination of vicagrel and its major metabolites in rat or human plasma: An optimized novel strategy for the stabilization of vicagrel
In this study, an optimized strategy was developed and validated to stabilize vicagrel, 2-oxo-clopidogrel (thiolactone metabolite), and H4 (active thiol metabolite) before quantification of the analytes, such as addition of citric acid (for plasma acidification) and NaF (a non-specific esterase inhibitor) to inhibit esterase activity, immediate addition of a thiol-alkylating reagent MPB into blood samples to derivatize H4 for the formation of stable H4 derivative (i.e., MP-H4), use of the anticoagulant K2EDTA to minimize the conversion of 2-oxo-clopidogrel to H-endo, and keeping the analytes at 4 °C or on wet ice to ...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 1, 2020 Category: Drugs & Pharmacology Source Type: research

Enantioselective separation of Novel Psychoactive Substances using a Lux® AMP 3 μm column and HPLC-UV
Publication date: 5 February 2020Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 179Author(s): Johannes S. Hägele, Martina Basrak, Martin G. SchmidAbstractThe abuse of Novel Psychoactive Substances (NPS) still cause severe problems. A lot of them contain a stereogenic centre which leads to possible different pharmacological effects of the enantiomers.The aim of this study was to establish an enantioselective HPLC-UV method with applicability to a broad spectrum of NPS. Different compound classes including mainly amphetamines, cathinones, ketamines, pyrovalerones and benzofuries were tested for succes...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 1, 2020 Category: Drugs & Pharmacology Source Type: research

Metabolomic study of raw and bran-fried Atractylodis Rhizoma on rats with spleen deficiency
This study showed that Atractylodis Rhizoma could provide satisfactory therapeutic effects on spleen deficiency and metabolomics study can be utilized to further understand the molecular mechanisms. (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - December 31, 2019 Category: Drugs & Pharmacology Source Type: research

A quality control system for ligand-binding assay of plasma renin activity: Proof-of-concept within a pharmacodynamic study
Publication date: Available online 28 December 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Fabian Konstantin Suessenbach, Nina Makowski, Martin Feickert, Tanja Gangnus, Jutta Tins, Bjoern Bengt Burckhardt, on behalf of the LENA consortiumAbstractWhile the role of plasma renin activity (PRA) in heart failure have been widely studied in adults, comprehensive data on pediatric heart failure remain lacking. This drawback is increasingly being addressed by academic research. Nevertheless, such pediatric investigations are commonly conducted only once due to ethical constraints. Therefore, the quality...
Source: Journal of Pharmaceutical and Biomedical Analysis - December 29, 2019 Category: Drugs & Pharmacology Source Type: research

Measurement of NLG207 (formerly CRLX101) nanoparticle-bound and released camptothecin in human plasma
Publication date: Available online 27 December 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Keith T. Schmidt, Cody J. Peer, Alwin D.R. Huitema, Monique D. Williams, Susan Wroblewski, Jan H.M. Schellens, Ravi A. Madan, William D. FiggAbstractCamptothecin (CPT), a potent inhibitor of topoisomerase I and HIF-1α, failed to demonstrate utility as an anti-cancer agent in early clinical trial investigations, primarily due to limited clinical activity and significant toxicity attributable to unfavorable physicochemical properties (e.g. low plasma solubility, pH-labile lactone ring). NLG207 (formerl...
Source: Journal of Pharmaceutical and Biomedical Analysis - December 28, 2019 Category: Drugs & Pharmacology Source Type: research

Toxicological effects of some antiparasitic drugs on equine liver glutathione S-Transferase enzyme activity
In this study, we report the effect of some benzimidazole drugs such as ricobendazole (RBZ), thiabendazole (TBZ), albendazole (ALBA) and oxfendazole (OFZ) on glutathione s-transferase (GST) enzyme activity. The kinetics studies, IC50 and Ki values of the tested drugs on GSTs enzyme activity were investigated. The obtained ranking of IC50 values were found to be approximately RBZ (53.31 μM, r2: 0.9778)
Source: Journal of Pharmaceutical and Biomedical Analysis - December 28, 2019 Category: Drugs & Pharmacology Source Type: research

Rapid and highly sensitive quantification of the anti-tuberculosis agents isoniazid, ethambutol, pyrazinamide, rifampicin and rifabutin in human plasma by UPLC-MS/MS
Publication date: Available online 26 December 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Lingjie Wu, Zhenjie Ye, Hui Liu, Hongliang Guo, Jing Lin, Ling Zheng, Nannan Chu, Xiaolong LiuAbstractWith the increased cases of multidrug- or rifampicin-resistant tuberculosis and co-infection with HIV globally, it is difficult to achieve ideal clinical responses because of poor drug absorption and drug-drug interactions. Herein, a bioanalytical UPLC-MS/MS method was developed and validated to quantify five anti-TB agents in human plasma samples for detecting blood drug concentrations to improve therapeu...
Source: Journal of Pharmaceutical and Biomedical Analysis - December 28, 2019 Category: Drugs & Pharmacology Source Type: research

Chemical profiling of Coptis rootlet and screening of its bioactive compounds in inhibiting Staphylococcus aureus by UPLC-Q-TOF/MS
This study proved the utility of the Coptis rootlet, which is beneficial to the more comprehensive utilization of medicinal plant resources.Graphical Abstract (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - December 28, 2019 Category: Drugs & Pharmacology Source Type: research

Metabolomics study to identify Plasma Biomarkers in Alzheimer Disease: ApoE genotype effect
In conclusion, ApoE4 genotype is associated with changes in lipid metabolomics profile in AD patients, and it could be relevant for the development of AD since early stages. (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - December 28, 2019 Category: Drugs & Pharmacology Source Type: research

Recent developments of bioanalytical methods in determination of neurotransmitters in vivo
Publication date: Available online 24 December 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Joanna Matys, Barbara Gieroba, Krzysztof JóźwiakAbstractNeurotransmitters (NTs) constitute an important group of messenger molecules and their imbalance lead to various neurological disorders, making their analytical determination of great importance in both laboratory and clinical practice. Here we review the most recent progress in sample pretreatment and in vivo analysis for various NTs and metabolites focusing on two approaches: biosensors and SPME, which require small amounts of biological sam...
Source: Journal of Pharmaceutical and Biomedical Analysis - December 25, 2019 Category: Drugs & Pharmacology Source Type: research