Chemical and metabolic analysis of Achyranthes bidentate saponins with intestinal microflora-mediated biotransformation by ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry coupled with metabolism platform
Publication date: Available online 28 March 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Jun Fu, Hong Wu, Huan Wu, Ran Deng, Feng LiAbstractAchyranthes bidentate (AB) is a typical traditional Chinese medicine (TCM) that has been widely used in clinical practices for more than a thousand years. Modern pharmacological studies have shown that triterpene saponins are the main pharmacological active ingredients in AB. Meanwhile, the poor oral bioavailability of triterpene saponins in AB indicates that these ingredients are probably metabolized by intestinal microflora before absorption. In this work, ...
Source: Journal of Pharmaceutical and Biomedical Analysis - March 29, 2019 Category: Drugs & Pharmacology Source Type: research

Development and validation of a sensitive LC-MS/MS method for determination of gefitinib and its major metabolites in human plasma and its application in non-small cell lung cancer patients
In this study, we established and validated a method for determining gefitinib and its main metabolites, M605211, M387783, M537194 and M523595 in patients with non-small cell lung cancer (NSCLC) by liquid chromatography–tandem mass spectrometry (LC-MS/MS) method. The mobile phase was water: acetonitrile (35:65, v/v) with 0.1% formic acid at a flow‐rate of 0.35 mL/min, within a 3 min run time. Gefitinib and its main metabolites were separated on a X-Terra RP18 column (50 × 2.1 mm, 3.5 μm) at 40 ℃ and subjected to mass analysis using positive electro-spray ionizati...
Source: Journal of Pharmaceutical and Biomedical Analysis - March 29, 2019 Category: Drugs & Pharmacology Source Type: research

A study on structural characterization of degradation products of cangrelor using LC/QTOF/MS/MS and NMR
Publication date: Available online 28 March 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Vinodh Guvvala, Venkatesan Chidambaram Subramanian, Jaya Shree AnireddyAbstractA complete degradation study was performed on cangrelor drug substance as per the ICH guidelines. The study reveals that a total of six degradation products (DP-1 to DP-6) were found and out of these, three unknown degradation products (DP-1, DP-5 and DP-6) were not reported in the literature. Based on the degradation study, the drug substance cangrelor was found to be sensitive towards acidic, basic and oxidative conditions. Besid...
Source: Journal of Pharmaceutical and Biomedical Analysis - March 29, 2019 Category: Drugs & Pharmacology Source Type: research

An optimized TaqMan real-time PCR method for authentication of ASINI CORII COLLA (donkey-hide gelatin)
In this study, probe/primers of high specificity and sensitivity were selected to analyze donkey-hide gelatin for donkey DNA and to look for horse, ox, and pig DNA as possible adulterants. The mitochondrial CO I genes in donkey, horse, and ox were selected as target sequences for design and synthesis of three pairs of specific probes and primers. In addition, eight pairs of probe/primers were obtained via literature search. Out of these eleven groups of probe/primers, those with the highest specificity and sensitivity were selected, which was fulfilled by the screening firstly with animal hide samples then the hide-glue sa...
Source: Journal of Pharmaceutical and Biomedical Analysis - March 29, 2019 Category: Drugs & Pharmacology Source Type: research

A sensitive and selective multiple reaction monitoring mass spectrometry method for simultaneous quantification of flavonol glycoside, terpene lactones, and biflavonoids in Ginkgo biloba leaves
In this study, an efficient and sensitive UHPLC-QQQ-MS/MS (MRM) analytical strategy was established firstly for simultaneous determination of 11 components, including 3 original flavonol glycoside, 4 terpene lactones and 4 biflavonoids in Ginkgo biloba leaves. The validated strategy exhibited proper linearity (R2 ≥ 0.99) in the range of 0.5-125 μg/mL, and intra and inter-day precision were lower than 4.09% and 4.80%, respectively. Limit of detection (LOD) and quantification (LOQ) were calculated, ranging from 0.2 to 4.6 ng/mL, with repeatability values between 1.98% and 4.48%. The average recoveries wer...
Source: Journal of Pharmaceutical and Biomedical Analysis - March 28, 2019 Category: Drugs & Pharmacology Source Type: research

Development and Validation of a Simple, Fast, and Sensitive LC/MS/MS Method for the Quantification of Oxfendazole in Human Plasma and Its Application to Clinical Pharmacokinetic Study
In this study, we have developed a high-performance liquid chromatography-tandem mass spectrometry (LC/MS/MS) method for the quantification of oxfendazole in human plasma using albendazole as an internal standard. The established method was fully validated with lower limit of quantitation (LLOQ) of 0.5 ng/mL and linearity in the range of 0.5 – 1000 ng/mL; intra-day and inter-day accuracies ranged from 2.6 to 9.5% for 3 quality control levels (1.5 ng/mL, 75 ng/mL, and 750 ng/mL) and LLOQ; intra-day and inter-day precision was ≤13.6% for quality controls and ≤15.1% for LLOQ; mat...
Source: Journal of Pharmaceutical and Biomedical Analysis - March 28, 2019 Category: Drugs & Pharmacology Source Type: research

Quantification of estradiol cypionate in plasma by liquid chromatography coupled with tandem mass spectrometry: Application in a pharmacokinetic study in healthy female volunteers
Publication date: Available online 26 March 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Roberto Salvador Martins, Natalícia J. Antunes, Guilherme Comerlatti, Gabriela Caraccio, Ronilson A. Moreno, Francesco Frecentese, Giuseppe Caliendo, Gilberto De NucciAbstractThe combination of medroxyprogesterone acetate 25 mg + estradiol cypionate 5 mg is a highly effective, monthly injectable contraceptive. For the first time, this study presents the development and validation of a sensitive method for estradiol cypionate analysis in human plasma by liquid chromatography ...
Source: Journal of Pharmaceutical and Biomedical Analysis - March 28, 2019 Category: Drugs & Pharmacology Source Type: research

Rapid multiresidue analysis of authorized/banned cyclopolypeptide antibiotics in feed by liquid chromatography-tandem mass spectrometry based on dispersive solid-phase extraction
Publication date: Available online 26 March 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Xuqin Song, Qiwen Huang, Yingxia Zhang, Meiyu Zhang, Jingmeng Xie, Limin HeAbstractAn effective analytical strategy was developed for simultaneous determination of seven cyclopolypeptide antibiotics (vancomycin, polymyxin B, polymyxin E, teicoplanin A2, bacitracin A, daptomycin and virginiamycin M1) in feed using liquid chromatography-tandem mass spectrometry. The extraction of sample was based on acidified methanol-aqueous solution, followed by a simply dispersive solid-phase extraction for further purificat...
Source: Journal of Pharmaceutical and Biomedical Analysis - March 28, 2019 Category: Drugs & Pharmacology Source Type: research

Determination of kansuiphorin C and kansuinin A in rat feces using UFLC-MS/MS and its application in the comparative excretion study on normal and malignant ascites rats
Publication date: Available online 26 March 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Yi Zhang, Jian-Wei Lou, Qiao Zhang, Zhen-Lan Li, Bei-Hua Bao, Yu-Dan Cao, Wei-Feng Yao, Yu-Ping Tang, Li ZhangAbstractMalignant ascites (MA) is one of the severe complications of gastrointestinal tumors, affecting the patients’ survival time and quality of life. Euphorbia kansui is a commonly used toxic Chinese herbal medicine for malignant ascites. Our previous study showed that the biological and toxicological effects of kansui were closely related to the gastrointestinal tract. The ingenane-type and ...
Source: Journal of Pharmaceutical and Biomedical Analysis - March 28, 2019 Category: Drugs & Pharmacology Source Type: research

New HPLC–MS method for rapid and simultaneous quantification of doxycycline, diethylcarbamazine and albendazole metabolites in rat plasma and organs after concomitant oral administration
Publication date: Available online 26 March 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Andi Dian Permana, Ismaiel A. Tekko, Helen O. McCarthy, Ryan F. DonnellyAbstractA sensitive and relatively fast, cost-effective high-performance liquid chromatographic method coupled with mass spectrometer (HPLC-MS) is herein reported for the first time for a simultaneous quantification of plasma and organs concentration of three therapeutic agents that are widely used in treatment of lymphatic filariasis (LF), namely, doxycycline (DOX), diethylcarbamazine (DEC) and albendazole (ABZ) metabolites. The method w...
Source: Journal of Pharmaceutical and Biomedical Analysis - March 28, 2019 Category: Drugs & Pharmacology Source Type: research

LC–MS/MS assay for the quantification of testosterone, dihydrotestosterone, androstenedione, cortisol and prednisone in plasma from castrated prostate cancer patients treated with abiraterone acetate or enzalutamide
Publication date: 5 June 2019Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 170Author(s): Merel van Nuland, Nikkie Venekamp, Willemijn M.E. Wouters, Huub H. van Rossum, Hilde Rosing, Jos H. BeijnenAbstractProstate cancer is the most common malignancy among men in the Western world. Treatment of this patient population, e.g. by (chemical) castration, is primarily focused on depletion of tumor-stimulating androgens, with testosterone being the major androgenic hormone. After initial therapy, prostate cancer may progress to metastatic castration-resistant prostate cancer. Anti-hormonal drugs abiraterone ace...
Source: Journal of Pharmaceutical and Biomedical Analysis - March 28, 2019 Category: Drugs & Pharmacology Source Type: research

Stability of meropenem in plasma versus dried blood spots (DBS)
Publication date: Available online 25 March 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Jens Martens-Lobenhoffer, Daniel Monastyrski, Uwe Tröger, Stefanie M. Bode-BögerAbstractMeropenem, a beta-lactam antibiotic belonging to the group of carbapenems, is widely used in the treatment of serious and complicated infections. Therapeutic drug monitoring (TDM) is strongly recommended to achieve therapeutic success, but the limited stability of the drug in plasma makes transport between clinic and laboratory difficult. The aim of this study was to investigate whether the stability of meropenem...
Source: Journal of Pharmaceutical and Biomedical Analysis - March 27, 2019 Category: Drugs & Pharmacology Source Type: research

Characterization and identification of the chemical constituents of Polygonum multiflorum Thunb. by high-performance liquid chromatography coupled with ultraviolet detection and linear ion trap FT-ICR hybrid mass spectrometry
This study provides a meaningful basis for correcting some mistakes in previous studies, as well as further quality control and pharmacological and toxicological research. (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - March 27, 2019 Category: Drugs & Pharmacology Source Type: research

Deciphering the intervention mechanism of Taohong Siwu Decoction following the abnormal uterine bleeding rats based on serum metabolic profiles
In this study, serum metabolic profile data was collected using ultra high-performance liquid chromatography with ion trap/time-of-flight mass spectrometry and gas chromatography-mass spectrometry. 23 potential biomarkers (urea, serine, L-proline, L-glutamic acid, palmitic acid, L-acetylcarnitine, LysoPC(16:0), LysoPC(20:4), L-proline, linoleic acid, stearic acid, L-isoleucine, phenylalanine, L-tyrosine, Oleic acid, et al) were eventually identified using multivariate statistical analysis (PCA and OPLS-DA) with VIP > 1, P 
Source: Journal of Pharmaceutical and Biomedical Analysis - March 25, 2019 Category: Drugs & Pharmacology Source Type: research

Pharmacokinetic Study on Bruceoside A Revealed the Potential Role of Quassinoid Glycosides for the Anticancer Properties of Fructus Bruceae
Publication date: Available online 23 March 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Yuan Xu, Mingming Xu, Lin Zhang, Zhenhua Zhu, Sheng Guo, Shulan Su, Jianming Guo, Chun-Tao Che, Zhi-Xiu Lin, Ming Zhao, Jin-Ao DuanAbstractBruceoside A, an abundant quassinoid glycoside in Fructus Bruceae, was chosen for the pharmacokinetic study. It is the first case report on the pharmacokinetic study of quassinoid glycosides so far. A sensitive, accurate, and repeatable UHPLC–MS/MS method was developed for the determination of bruceoside A and its major metabolite. The results showed bruceoside A cou...
Source: Journal of Pharmaceutical and Biomedical Analysis - March 25, 2019 Category: Drugs & Pharmacology Source Type: research

Target Exposure and Pharmacodynamics Study of the Indoleamine 2,3-Dioxygenase-1 (IDO-1) Inhibitor Epacadostat in the CT26 Mouse Tumor Model
In this study, using QMSI, we went further than only quantifying the metabolites and the drug, by estimating the pharmacological effect efficacy of the drug through a target exposure study handled in different regions of the tumor either expressing IDO1 or Kyn.Graphical abstract (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - March 23, 2019 Category: Drugs & Pharmacology Source Type: research

Dual enantioselective LC–MS/MS method to analyse chiral drugs in surface water: Monitoring in Douro River estuary
Publication date: 5 June 2019Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 170Author(s): Maria Miguel Coelho, Ana Rita Lado Ribeiro, João C.G. Sousa, Cláudia Ribeiro, Carla Fernandes, Adrián M.T. Silva, Maria Elizabeth TiritanAbstractThis work presents the development of an enantioselective method to quantify chiral drugs (CDs) in surface water and its application in the Douro River estuary monitoring. Different classes of CDs were targeted, including 23 compounds, namely beta-blockers, antidepressants, one beta2-adrenergic agonist, non-steroidal anti-inflammatory drugs, stimulants,...
Source: Journal of Pharmaceutical and Biomedical Analysis - March 23, 2019 Category: Drugs & Pharmacology Source Type: research

A novel tool for structure assignment of hydroxylated metabolites of (arylpiperazinylbutyl)oxindole derivatives based on relative HPLC retention times
Publication date: 5 June 2019Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 170Author(s): Éva Szabó, Györgyi Koványi-Lax, Gábor Szénási, András Dancsó, Loránd Kiss, Róbert Kormány, Gyula Simig, Gábor Németh, Balázs VolkAbstractIncubation of oxindole derivatives containing an arylpiperazine pharmacophore in rat liver microsomes in vitro formed several metabolites hydroxylated at various positions of the aromatic rings of the oxindole carbocycle or the arylpiperazine moiety. In order to substitute the sit...
Source: Journal of Pharmaceutical and Biomedical Analysis - March 23, 2019 Category: Drugs & Pharmacology Source Type: research

Simultaneous Quantification of Tegoprazan and its Major Metabolite M1 in Dog Plasma using Liquid Chromatography-Tandem Mass Spectrometry
In this study, we developed and validated a simple bioanalytical liquid chromatography/tandem mass spectrometry (LC-MS/MS) method for the simultaneous quantification of tegoprazan and its major metabolite (M1) in dog plasma. The developed method is based on protein precipitation and LC-MS/MS. The method was validated based on regulatory guidance for bioanalytical method validation. The calibration curves were linear in the concentration range of 50 ng/mL–50 µg/mL for tegoprazan and 5 ng/mL–5 µg/mL for M1. The inter- and intra-day precisions were evaluated with a coefficient of variation of
Source: Journal of Pharmaceutical and Biomedical Analysis - March 22, 2019 Category: Drugs & Pharmacology Source Type: research

Elimination of Ghost Peaks by Optimization of Anion Exchange Chromatography Method for Determination of gamma-Carboxyglutamic acid (Gla)-domainless Impurity in Recombinant Activated Clotting Factor VII Drug Products
Publication date: Available online 22 March 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Hamed Tahmasbi, Nahid Zarezadeh, Somaie Marjaninia, Behnaz Molavi, Shayan Maleknia, Fereidoun Mahboudi, Hossein BehrouzAbstractSecreted recombinant activated clotting factor VII activated (rFVIIa) in cell culture media missing gamma-carboxyglutamic acid (Gla) domain as a result of failure in gamma-carboxylation or cell lysis is called Gla-domainless impurity which has less negative charge compared to native rFVIIa. Based on risk assessment, this type of impurity is considered as critical drug product quality ...
Source: Journal of Pharmaceutical and Biomedical Analysis - March 22, 2019 Category: Drugs & Pharmacology Source Type: research

Development and application of a high-throughput liquid chromatography–tandem mass spectrometric method for the simultaneous determination of thymosin α1 and its recombinant human form in plasma and urine
Publication date: 5 June 2019Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 170Author(s): Tingting Wang, Lei Yin, Hao Wang, John Paul Fawcett, Jingkai GuAbstractThymosin α1 (Thymalfasin, Tα1) is a naturally occurring polypeptide widely used as an immune system enhancer for the treatment of HIV/AIDS, hepatitis B and C, and cancer. Recombinant human Tα1 (rh-Tα1) lacking N-terminal acetylation (NTA) displays similar biological activity to Tα1 and has completed phase III clinical trials in China. To compare the pharmacokinetics of rh-Tα1 and Tα1 and establish whether...
Source: Journal of Pharmaceutical and Biomedical Analysis - March 22, 2019 Category: Drugs & Pharmacology Source Type: research

Identification of minor lignans, alkaloids, and phenylpropanoid glycosides in Magnolia officinalis by HPLC‒DAD‒QTOF-MS/MS
Publication date: Available online 19 March 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Keke Guo, Chaoying Tong, Qiachi Fu, Jinju Xu, Shuyun Shi, Yecheng XiaoABSTRACTAn effective strategy based on high-speed counter-current chromatography (HSCCC) knockout combination with HPLC–DAD–QTOF-MS/MS analysis were developed to identify minor lignans, alkaloids, and phenylpropanoid glycosides in M. officinalis. Petroleum ether/ethyl acetate/methanol/water (8:4:7:5, v/v/v/v) as solvent system was firstly selected to separate the crude extract of M. officinalis. Two major lignans, honokiol and m...
Source: Journal of Pharmaceutical and Biomedical Analysis - March 20, 2019 Category: Drugs & Pharmacology Source Type: research

Quantitative Determination and Pharmacokinetic Study of Fusaricidin A in Mice Plasma and Tissues using Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry
Publication date: Available online 19 March 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Mona H. Haron, Bharathi Avula, Qiu Shi, Xing-Cong Li, Mohammad K. Ashfaq, Ji-Yeong Bae, Shaohua Guan, Maud Hinchee, Ikhlas A. Khan, Shabana I. KhanAbstractFusaricidins are a family of cyclic lipodepsipeptides that convey antifungal and antibacterial activity. Fusaricidin A is one of the Fusaricidins major compounds and it is showing promising activity against fungi and bacteria. In the present study, a fast and sensitive ultra-high performance liquid chromatography coupled to triple quadrupole mass spectromet...
Source: Journal of Pharmaceutical and Biomedical Analysis - March 20, 2019 Category: Drugs & Pharmacology Source Type: research

Pharmacokinetics and metabolism of GW9508 in rat by liquid chromatography/electrospray ionization tandem mass spectrometry
This study provided an overview of disposition of GW9508, which is highly instructive for better understanding the effectiveness and toxicity of this drug. (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - March 20, 2019 Category: Drugs & Pharmacology Source Type: research

Analytical human biomonitoring method for the identification and quantification of the metabolite BDCPP originated from the organophosphate flame retardant TDCPP in urine
Publication date: Available online 19 March 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Petra Krystek, Henry Beeltje, Maarten Noteboom, Eric M. van den Hoeven, Marc M.G. HoutzagerAbstractTris(1,3-dichloropropyl) phosphate (TDCPP, CAS 13674-87-8) is one of the most commonly used organophosphate flame retardants (OPFRs) in cars, residential furniture and other products containing polyurethane foam to meet the required flammability standards. For the tasks of the working group Analyses in Biological Material from the German Research Foundation (DFG), a human biomonitoring process for TDCPP is devel...
Source: Journal of Pharmaceutical and Biomedical Analysis - March 20, 2019 Category: Drugs & Pharmacology Source Type: research

LC-MS/MS assay for the quantification of testosterone, dihydrotestosterone, androstenedione, cortisol and prednisone in plasma from castrated prostate cancer patients treated with abiraterone acetate or enzalutamide
Publication date: Available online 20 March 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Merel van Nuland, Nikkie Venekamp, Willemijn M.E. Wouters, Huub H. van Rossum, Hilde Rosing, Jos H. BeijnenAbstractProstate cancer is the most common malignancy among men in the Western world. Treatment of this patient population, e.g. by (chemical) castration, is primarily focused on depletion of tumor-stimulating androgens, with testosterone being the major androgenic hormone. After initial therapy, prostate cancer may progress to metastatic castration-resistant prostate cancer. Anti-hormonal drugs abirater...
Source: Journal of Pharmaceutical and Biomedical Analysis - March 20, 2019 Category: Drugs & Pharmacology Source Type: research

Development and validation of a green method for dissolution monitoring of pharmaceutical combinations. meloxican and pridinol
Publication date: Available online 18 March 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Duvernis Salazar-Rojas, Aldana Intilangelo, Silvana E. Vignaduzzo, Rubén M. MaggioAbstractThe development of a chemometric method for monitoring the pharmaceutical dissolution, under green analytical chemistry principles, was reported. Meloxicam (MEL) and pridinol (PRI) were employed as a combination model.Multivariate curve resolution with alternating least squares (MCR-ALS) was proposed to resolve UV spectra of the analytes during pharmaceutical dissolution. Empowering UV-Vis spectrophotometry, which...
Source: Journal of Pharmaceutical and Biomedical Analysis - March 20, 2019 Category: Drugs & Pharmacology Source Type: research

Monitoring of kynurenine pathway metabolites, neurotransmitters and their metabolites in blood plasma and brain tissue of individuals with latent toxoplasmosis
Publication date: Available online 18 March 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Jana Vondroušová, Miloš Mikoška, Kamila Syslová, Adéla Böhmová, Hana Tejkalová, Lukáš Vacek, Petr Kodym, Daniel Krsek, Jiří HoráčekAbstractThe aim of the presented work was to develop a highly sensitive, accurate and rapid analytical method for the determination of concentration levels of tryptophan and its metabolites of kynurenine catabolic pathway, as well as neurotransmitters and their metabolites in complex biologi...
Source: Journal of Pharmaceutical and Biomedical Analysis - March 20, 2019 Category: Drugs & Pharmacology Source Type: research

A validated UPLC-MS/MS method to determine free and total irinotecan and its two metabolites in human plasma after intravenous administration of irinotecan hydrochloride liposome injection
Publication date: Available online 18 March 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Quankun Zhuang, Xuemei Liu, Zhuo Sun, Hongyun Wang, Ji JiangAbstractIrinotecan hydrochloride liposome injection (IHLI) is a formulation of anticancer drug irinotecan hydrochloride (CPT-11) entrapped in the aqueous core of liposomes. To understand the pharmacokinetic property and evaluate the relationship between pharmacokinetics and pharmacodynamics/toxicity of IHLI, it is of prime importance to determine the concentrations of free CPT-11, total CPT-11 and its main metabolites (SN-38 and SN-38 G) in hu...
Source: Journal of Pharmaceutical and Biomedical Analysis - March 20, 2019 Category: Drugs & Pharmacology Source Type: research

Development of a colloidal gold-based lateral flow dipstick immunoassay for rapid detection of chlorogenic acid and luteoloside in Flos Lonicerae Japonicae
Publication date: Available online 18 March 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Bo Zhang, Tie-Gui Nan, Jie Xin, Zhi-Lai Zhan, Li-Ping Kang, Yuan Yuan, Bao-Min Wang, Lu-Qi HuangAbstractFlos Lonicerae Japonicae (FLJ), known as golden-and-silver honeysuckle, is a widely used Chinese herbal medicine with pharmacological activities and edibleness in China. Chlorogenic acid (CGA) and luteoloside are the quality control markers of FLJ regulated by the Chinese Pharmacopoeia (2015 edition). For rapid evaluation of the quality of FLJ, dipsticks for CGA and luteoloside detection were developed. The...
Source: Journal of Pharmaceutical and Biomedical Analysis - March 20, 2019 Category: Drugs & Pharmacology Source Type: research

Development and validation of a reverse phase HPLC method for SHetA2, a novel anti-cancer drug, in mouse biological samples
Publication date: Available online 18 March 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Manolya Kukut Hatipoglu, Sanjida Mahjabeen, Lucila Garcia-ContrerasAbstractSHetA2 is a flexible heteroarotinoid that has the potential to prevent and treat lung, ovarian and cervical cancer without significant toxicity. A simple and reliable high performance liquid chromatographic (HPLC) method was developed to determine SHetA2 concentrations in the lungs, reproductive organs and plasma of mice. SHetA2 was extracted from these biological matrices by solid phase and liquid-liquid extraction in the presence of ...
Source: Journal of Pharmaceutical and Biomedical Analysis - March 19, 2019 Category: Drugs & Pharmacology Source Type: research

Dilute and shoot ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC–MS/MS) analysis of psychoactive drugs in oral fluid
Publication date: Available online 16 March 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Sara Malaca, Francesco Paolo Busardò, Massimo Gottardi, Simona Pichini, Emilia MarcheiAbstractA fast and sensitive ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC–MS/MS) method was developed and validated for the measurement of the most common drugs of abuse and some new psychoactive substances in oral fluid.The target compounds were 6-monoacetylmorphine, morphine, codeine, cocaine, benzoylecgonine, amphetamine, methamphetamine, 3,4-methylenedioxymethamphetamine, &Del...
Source: Journal of Pharmaceutical and Biomedical Analysis - March 17, 2019 Category: Drugs & Pharmacology Source Type: research

Rapid screening and characterization of triterpene saponins in Acanthopanax senticosus leaves via untargeted MSAll and SWATH techniques on a quadrupole time of flight mass spectrometry
This study aimed to systematically analyze TSs in leaves of A. senticosus, and the results are significant for the utilization of A. senticosus leaves. (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - March 17, 2019 Category: Drugs & Pharmacology Source Type: research

Purification of drug degradation products supported by analytical and preparative supercritical fluid chromatography
Publication date: Available online 16 March 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Angéline Noireau, Elise Lemasson, Fabien Mauge, Anne-Marie Petit, Sophie Bertin, Philippe Hennig, Éric Lesellier, Caroline WestAbstractA stressed degradation (oxidation) was employed to produce metabolites from an active pharmaceutical ingredient (API) with large molecular weight (about 900 g/mol). An analytical chromatographic method was desired to compare the products generated by different degradation methods while a multi-gram-scale preparative chromatographic method was necessary to ...
Source: Journal of Pharmaceutical and Biomedical Analysis - March 17, 2019 Category: Drugs & Pharmacology Source Type: research

Multidirectional insights into the biochemical and toxicological properties of Bougainvillea glabra (Choisy.) aerial parts: A functional approach for bioactive compounds
Publication date: Available online 16 March 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Hammad Saleem, Gokhan Zengin, Irshad Ahmad, Joash Tan Ban Lee, Thet Thet Htar, Fawzi M. Mahomoodally, Rakesh Naidu, Nafees AhemadAbstractThe current research work was conducted in order to probe into the biochemical and toxicological characterisation of methanol and dichloromethane (DCM) extracts of Bougainvillea glabra (Choisy.) aerial parts. Biological fingerprints were assessed for in vitro antioxidant, key enzyme inhibitory and cytotoxicity potential. Total bioactive contents were determined spectrophotom...
Source: Journal of Pharmaceutical and Biomedical Analysis - March 17, 2019 Category: Drugs & Pharmacology Source Type: research

Editorial Board
Publication date: 10 May 2019Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 168Author(s): (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - March 17, 2019 Category: Drugs & Pharmacology Source Type: research

Development and application of a high-throughput liquid chromatography-tandem mass spectrometric method for the simultaneous determination of thymosin α1 and its recombinant human form in plasma and urine
Publication date: Available online 15 March 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Tingting Wang, Lei Yin, Hao Wang, John Paul Fawcett, Jingkai GuAbstractThymosin α1 (Thymalfasin, Tα1) is a naturally occurring polypeptide widely used as an immune system enhancer for the treatment of HIV/AIDS, hepatitis B and C, and cancer. Recombinant human Tα1 (rh-Tα1) lacking N-terminal acetylation (NTA) displays similar biological activity to Tα1 and has completed phase III clinical trials in China. To compare the pharmacokinetics of rh-Tα1 and Tα1 and establish ...
Source: Journal of Pharmaceutical and Biomedical Analysis - March 16, 2019 Category: Drugs & Pharmacology Source Type: research

An Optimized TaqMan Real-time PCR Method for Authentication of ASINI CORII COLLA(Donkey-hide Gelatin)
In this study, probe/primers of high specificity and sensitivity were selected to analyze donkey-hide gelatin for donkey DNA and to look for horse, ox, and pig DNA as possible adulterants. The mitochondrial CO I genes in donkey, horse, and ox were selected as target sequences for design and synthesis of three pairs of specific probes and primers. In addition, eight pairs of probe/primers were obtained via literature search. Out of these eleven groups of probe/primers, those with the highest specificity and sensitivity were selected, which was fulfilled by the screening firstly with animal hide samples then the hide-glue sa...
Source: Journal of Pharmaceutical and Biomedical Analysis - March 16, 2019 Category: Drugs & Pharmacology Source Type: research

Dual enantioselective LC-MS/MS method to analyse chiral drugs in surface water: monitoring in Douro River estuary
Publication date: Available online 15 March 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Maria Miguel Coelho, Ana Rita Lado Ribeiro, João C.G. Sousa, Cláudia Ribeiro, Carla Fernandes, Adrián M.T. Silva, Maria Elizabeth TiritanAbstractThis work presents the development of an enantioselective method to quantify chiral drugs (CDs) in surface water and its application for Douro River estuary monitoring. Different classes of CDs were targeted, including 23 compounds, namely beta-blockers, antidepressants, one beta2-adrenergic agonist, non-steroidal anti-inflammatory drugs, stimula...
Source: Journal of Pharmaceutical and Biomedical Analysis - March 16, 2019 Category: Drugs & Pharmacology Source Type: research

Co-existing colloidal phases of human duodenal aspirates: Intraindividual fluctuations and interindividual variability in relation to molecular composition
Publication date: Available online 15 March 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Philipp A. Elvang, Mette Sloth Bohsen, Paul C. Stein, Annette Bauer-Brandl, Danny Riethorst, Joachim Brouwers, Patrick Augustijns, Martin BrandlAbstractWe investigated the ultrastructural pattern of colloidal phases in human duodenal fluids. Aspirates were collected from three volunteers in both fasted and fed nutritional states. Analysis methods comprised the combination of asymmetric flow field-flow fractionation (AF4) and multi-angle laser light scattering (MALLS). Furthermore, dynamic light scattering (DL...
Source: Journal of Pharmaceutical and Biomedical Analysis - March 16, 2019 Category: Drugs & Pharmacology Source Type: research

Efficient separation determination of protopanaxatriol ginsenosides Rg1, Re, Rf, Rh1, Rg2 by HPLC
Publication date: Available online 15 March 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Lina Zhang, Shaoyan Wang, Boquan Qu, Haijun Chi, Yanling Quan, Xiuhong WuAbstractAn efficient and flexible protocol was developed for simultaneous detection of the ginsenosides Rg1, Re, Rf, Rh1, and Rg2 in ginseng extract. In the analysis of white ginseng that contains no ginsenoside Rf, separation of the remaining ginsenosides was achieved within just 10 min under isocratic chromatographic conditions. For the analysis of red ginseng that contains ginsenoside Rf as a characteristic constituent, the gradient e...
Source: Journal of Pharmaceutical and Biomedical Analysis - March 16, 2019 Category: Drugs & Pharmacology Source Type: research

Identification of eupatilin and ginkgolide B as p38 ligands from medicinal herbs by surface plasmon resonance biosensor-based active ingredients recognition system
In this study, a surface plasmon resonance biosensor-based active ingredient recognition system (SPR-AIRS) was applied to screen p38 mitogen-activated protein kinase (p38) ligands from herbal extracts. After p38 protein was immobilized on a SPR chip and the suitability of SPR-AIRS was validated, thirty-four p38-related medicinal herbs were selected and pre-screened. Two medicinal herbs having high response signal with p38-immobilized chip, Folium Ginkgo and Herba Artemisiae Scopariae, were injected into SPR system for ligand fishing. Among them, two active compounds, eupatilin (EPT) and ginkgolide B (GKB), were identified ...
Source: Journal of Pharmaceutical and Biomedical Analysis - March 16, 2019 Category: Drugs & Pharmacology Source Type: research

Simultaneous determination of calycosin-7-O-β-D-glucoside, cinnamic acid, paeoniflorin and albiflorin in rat plasma by UHPLC-MS/MS and its application to a pharmacokinetic study of Huangqi Guizhi Wuwu Decoction
Publication date: Available online 14 March 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Jiao Guan, Liming Wang, Jia Jin, Sheng Chang, Xiao Xiao, Bo Feng, Heyun ZhuAbstractHuangqi Guizhi Wuwu Decoction (HGWWD), consisting of Radix Astragali, Cinnamomi Ramulus, Paeoniae Radix Alba, Zingiberis Rhizoma Recens and Jujubae Fructus, is a widely used Traditional Chinese Medicine (TCM) formula for the treatment of human blood impediment in China for nearly 2000 years. In order to make good and rational use of this formula in the future, a rapid, sensitive and robust ultra-high performance liquid chromato...
Source: Journal of Pharmaceutical and Biomedical Analysis - March 15, 2019 Category: Drugs & Pharmacology Source Type: research

Development of a liquid chromatography-tandem mass spectrometric (LC-MS/MS) method for simultaneous determination of epigallocatechin-3-gallate, silibinin, and curcumin in plasma and different tissues after oral dosing of Protandim in rats and its application in pharmacokinetic and tissue distribution studies
Publication date: Available online 14 March 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Ying Song, Huijing Sun, Jie Xiao, Fan Wang, Yi Ding, Jinyi Zhao, Juan Bai, Lianghua Cheng, Kai Gao, Meiyou Liu, Qiyan Guo, Yanmin Zhang, Gao Wei, Yanyan Jia, Aidong WenAbstractProtandim is an over-the-counter herbal dietary supplement. The key components of Protandim, i.e., epigallocatechin-3-gallate (EGCG), silibinin (SIL), and curcumin (CUR) were simultaneously analyzed through a liquid chromatography-tandem mass spectrometric (LC-MS/MS) method in plasma and different tissues after administration of Protand...
Source: Journal of Pharmaceutical and Biomedical Analysis - March 15, 2019 Category: Drugs & Pharmacology Source Type: research

Potential pitfalls in LC-MS/MS quantification of colistin for therapeutic drug monitoring of patients treated with colistimethate
Publication date: Available online 14 March 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Sebastiano Barco, Elio Castagnola, Alessio Mesini, Gino Tripodi, Giuliana CangemiAbstractStarting from a warning of possible artificially high plasma colistin concentrations observed in clinical samples in our recently published LC-MS/MS method, we reviewed our analytical assay and demonstrated, for the first time, that caution must be paid to ionization conditions employed in the MS system.The artefact reported here demonstrates that additional experiments should be performed in the course of validation of L...
Source: Journal of Pharmaceutical and Biomedical Analysis - March 15, 2019 Category: Drugs & Pharmacology Source Type: research

Chemometric study of the excipients’ influence on polymorphic-behavior. Mefenamic acid as case of study
Publication date: Available online 14 March 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Marina Antonio, Natalia L. Calvo, Rubén M. MaggioAbstractThe assessment of polymorphism is a problematical issue for regulatory agencies, because variations among crystalline forms of active pharmaceutical ingredient (API) can lead to changes in the efficacy and safety of formulated product. Such conversions are very hard to be detected, thus, the development of techniques for the identification, characterization and quantification of polymorphs results essential in all stages of the manufacturing proc...
Source: Journal of Pharmaceutical and Biomedical Analysis - March 15, 2019 Category: Drugs & Pharmacology Source Type: research

RRLC-DAD-ESI-MS based and bioactivity guided phytochemical analysis and separation of coumarins from raw extracts of Trigonostemon lutescens
Publication date: Available online 13 March 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Chang-shui Yang, Su-qiao Han, Xing Wang, Tong Zhou, Xiao-yun Dong, Ping BoAbstractAs extensively active compounds, coumarins are rarely reported on the phytochemistry of the genus Trigonostemon. We herein proposed a fast strategy for analysis and separation of antitumoral active coumarins from the twigs of T. lutescens. Rapid Resolution liquid chromatography coupled with diode array detection and electrospray ionization mass spectrometry (RRLC-DAD-ESI-MS) analysis indicated the existence of coumarins in the t...
Source: Journal of Pharmaceutical and Biomedical Analysis - March 13, 2019 Category: Drugs & Pharmacology Source Type: research

Development of an integrated chromatographic system for ω-transaminase-IMER characterization useful for flow-chemistry applications
Publication date: Available online 11 March 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): M. Corti, F. Rinaldi, D. Monti, E.E. Ferrandi, G. Marrubini, C. Temporini, G. Tripodo, T. Kupfer, P. Conti, M. Terreni, G. Massolini, E. CalleriAbstractAn integrated chromatographic system was developed to rapidly investigate the biocatalytic properties of ω-transaminases useful for the synthesis of chiral amines. ATA-117, an (R)-selective ω-transaminase was selected as a proof of concept. The enzyme was purified and covalently immobilized on an epoxy monolithic silica support to create an immobil...
Source: Journal of Pharmaceutical and Biomedical Analysis - March 12, 2019 Category: Drugs & Pharmacology Source Type: research

A novel tool for structure assignment of hydroxylated metabolites of (arylpiperazinylbutyl) oxindole derivatives based on relative HPLC retention times
Publication date: Available online 11 March 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Éva Szabó, Györgyi Koványi-Lax, Gábor Szénási, András Dancsó, Loránd Kiss, Róbert Kormány, Gyula Simig, Gábor Németh, Balázs VolkAbstractIncubation of oxindole derivatives containing an arylpiperazine pharmacophore in rat liver microsomes in vitro formed several metabolites hydroxylated at various positions of the aromatic rings of the oxindole carbocycle or the arylpiperazine moiety. In order to substitute ...
Source: Journal of Pharmaceutical and Biomedical Analysis - March 12, 2019 Category: Drugs & Pharmacology Source Type: research

Sensitive fluorescent detection of Listeria monocytogenes by combining a universal asymmetric polymerase chain reaction with rolling circle amplification
Publication date: Available online 9 March 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Zhongxu Zhan, Ju Liu, Leina Yan, Zoraida P. Aguilar, Hengyi XuAbstractA new, facile, low-cost, and highly sensitive method for detection of Listeria monocytogenes involving a combination of asymmetric polymerase chain reaction (aPCR) and rolling circle amplification (RCA) had been developed. The aPCR-RCA processes were not new but components of the processes made the assay useful. Twenty-one thymine (21-T) tagged forward primer generated universal twenty-one adenine (21-A) aPCR amplicons after aPCR amplificati...
Source: Journal of Pharmaceutical and Biomedical Analysis - March 11, 2019 Category: Drugs & Pharmacology Source Type: research