Comprehensive quality assessment for Rhizoma Coptidis based on quantitative and qualitative metabolic profiles using high performance liquid chromatography, Fourier transform near-infrared and Fourier transform mid-infrared combined with multivariate statistical analysis
In this study, a comprehensive quality assessment for RC medicines from C. chinensis, C. deltoidea, C. omeiensis and C. teeta species was performed based on quantitative and qualitative metabolic profiles obtained from high performance liquid chromatography (HPLC), Fourier transform near-infrared (FT-NIR) and Fourier transform mid-infrared (FT-MIR) combined with multivariate statistical analysis. Eight alkaloids including magnoflorine, groenlandicine, jatrorrhizine, columbamine, epiberberine, coptisine, palmatine and berberine were simultaneously identified and determined. Epiberberine, berberine, magnoflorine and groenlan...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 6, 2018 Category: Drugs & Pharmacology Source Type: research

Hydrochlorothiazide binding to human serum albumin induces some compactness in the molecular structure of the protein: A multi-spectroscopic and computational study
Publication date: Available online 5 September 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Fatemeh Balaei, Sirous GhobadiAbstractThe interaction between hydrochlorothiazide (HCTZ), a diuretic drug, with human serum albumin (HSA) was investigated by different biophysical approaches such as UV absorption, circular dichroism (CD), Fourier transform infrared (FT-IR), and fluorescence spectroscopy in 50 mM sodium phosphate buffer, pH 7.4. The results of fluorescence titration experiments revealed that HCTZ strongly quenches the intrinsic fluorescence of HSA through a static quenching mechanism...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 6, 2018 Category: Drugs & Pharmacology Source Type: research

Determination of xanthones and flavonoids of methanol extracts obtained from different parts of the plants of three Gentianaceae species
Publication date: Available online 5 September 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Cheng-Yong Feng, Qian Wu, Dan-Dan Yin, Bing Li, Shan-Shan Li, Zhong-Qiu Tang, Yan-Jun Xu, Liang-Sheng WangAbstractGentianopsis barbata, Halenia corniculata, and Gentianella acuta were widely distributed throughout China and commonly used in traditional Chinese medicine. However, owing to similar living environments and morphological features, locals often had trouble distinguishing between these three species. In this present study, chromatograms at 350 nm were obtained and the composition and conte...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 6, 2018 Category: Drugs & Pharmacology Source Type: research

Pharmacokinetics and tissue distribution of N-(2-hydroxyphenyl)-2-propylpentanamide in Wistar Rats and its binding properties to human serum albumin
Publication date: Available online 5 September 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Ana María Correa-Basurto, Aurelio Romero-Castro, José Correa-Basurto, Maricarmen Hernández-Rodríguez, Marvin Antonio Soriano-Ursúa, Jazmin García-Machorro, Luis Esteban Tolentino-López, Martha Cecilia Rosales-Hernández, Jessica Elena Mendieta-WejebeAbstractN-(2-hydroxyphenyl)-2-propylpentanamide (HO-AAVPA) is a novel valproic acid derivative that has shown anti-proliferative activity against epitheloid cervix carcinoma (HeLa), rhabdomyosarcoma (A204),...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 6, 2018 Category: Drugs & Pharmacology Source Type: research

Quantification of isoflavone glycosides and aglycones in rat plasma by LC-MS/MS: Troubleshooting of interference from food and its application to pharmacokinetic study of Semen Sojae Praeparatum extract
Publication date: Available online 6 September 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Yuan Yao, Xin Ma, Teng Li, Hui Guo, Ruirui Chang, Jialin Liu, Qiaoxia Liu, Hongyuan Hao, Taohong Huang, Weidong Chen, Jun Wen, Tingting ZhouAbstractThe isoflavones widely exist in the daily diets and interferences are usually inevitable in the determination of the in vivo level of the same analytes. A new strategy to eliminate the dietary interference was established to evaluate the exposure of isoflavones including daidzin, glycitin, genistin, daidzein, glycitein, and genistein in rats fed with Semen Soja...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 6, 2018 Category: Drugs & Pharmacology Source Type: research

Fast simultaneous determination of main components and impurity sodium ion in PAMA injection by mixed-mode chromatography
This study describes an analytical method to control the quality of potassium aspartate and magnesium aspartate (PAMA) injection based on the simultaneous detection of the main components (K+, Mg2+ and Asp) and impurities (Na+) using a mixed-mode chromatography coupled with charged aerosol detector. To obtain optimal chromatographic separation, the effects of organic content, column temperature, buffer types, pH and concentrations were evaluated. A Response Surface Methodology (RSM) optimal design was performed after single factor experiment. The mixed-mode HPLC method is proved to be a complementary approach to the conven...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 4, 2018 Category: Drugs & Pharmacology Source Type: research

Investigation of the inhibition effect of arachidonic acid on the core structure of the HIV-1 gp41
Publication date: Available online 3 September 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Xiaoying Xu, Li Lin, Zhen Liu, Xiaojun Yao, Xuanxuan Zhang, Shuwen Liu, Lihong LiuAbstractThe gp41 transmembrane domain of the envelope glycoprotein of the human immunodeficiency virus (HIV) modulates the conformation of the viral envelope spike. During the HIV fusion process, C-terminal heptad repeat (CHR, C34) wrap antiparallel to the N-terminal heptad repeat (NHR, N36) helices to form a stable six-helix bundle (6-HB) core structure, which brings the viral and cell membranes into close proximity for fusi...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 4, 2018 Category: Drugs & Pharmacology Source Type: research

SFC for chiral separations in bioanalysis
Publication date: Available online 3 September 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Lukas C. Harps, Jan F. Joseph, Maria K. ParrAbstractThis review covers literature investigating methods for enantioselective chromatography using supercritical fluids as mobile phase constituents (SFC) in the field of bioanalysis. It provides an overview on method development and screening approaches published in scientific literature 2014-2018. Chiral stationary phases are used to create a chiral environment that allows for discrimination of enantiomers. Especially polysaccharide-based stationary phases a...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 3, 2018 Category: Drugs & Pharmacology Source Type: research

A Chemical Derivatization Based UHPLC-LTQ-Orbitrap Mass Spectrometry Method for Accurate Quantification of Short-Chain Fatty Acids in Bronchoalveolar Lavage Fluid of Asthma Mice
Publication date: Available online 1 September 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Ying-Ying Jin, Zi-Qi Shi, Wen-Qi Chang, Lin-Xiu Guo, Jian-Liang Zhou, Jian-Qun Liu, Li-Fang Liu, Gui-Zhong XinAbstractRecent studies have demonstrated the important role of short-chain fatty acids (SCFAs) in the maintenance of homeostasis of respiratory immunity. However, there is still no report focus on the determination of SCFAs level in bronchoalveolar lavage fluid (BALF), the most common sample used for screening biomarkers of the pulmonary diseases. Herein, an ultra-high-performance liquid chromatogr...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 2, 2018 Category: Drugs & Pharmacology Source Type: research

Recent advances in designing nanomaterial based biointerfaces for electrochemical biosensing cardiovascular biomarkers
Publication date: Available online 1 September 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Leila Farzin, Mojtaba Shamsipur, Leila Samandari, Shahab SheibaniAbstractEarly diagnosis of cardiovascular disease (CVD) is critically important for successful treatment and recovery of patients. At present, detection of CVD at early stages of its progression becomes a major issue for world health. The nanoscale electrochemical biosensors exhibit diverse outstanding properties, rendering them extremely suitable for the determination of CVD biomarkers at very low concentrations in biological fluids. The uni...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 2, 2018 Category: Drugs & Pharmacology Source Type: research

Editorial Board
Publication date: 25 October 2018Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 160Author(s): (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - September 2, 2018 Category: Drugs & Pharmacology Source Type: research

Preformulation studies of finasteride to design matrix systems for topical delivery
Publication date: Available online 29 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Stephania G.B. Lima, Ludmila A.G. Pinho, Maíra N. Pereira, Tais Gratieri, Livia Lira Sa-Barreto, Guilherme M. Gelfuso, Marcilio Cunha-FilhoGraphical abstract (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - August 30, 2018 Category: Drugs & Pharmacology Source Type: research

13C-NMR-based metabolic fingerprinting of Citrus-type crude drugs
Publication date: Available online 28 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Takashi Tsujimoto, Taichi Yoshitomi, Takuro Maruyama, Yutaka Yamamoto, Takashi Hakamatsuka, Nahoko UchiyamaAbstractFive Citrus-type crude drugs (40 samples) were classified using 13C-NMR spectra-based metabolomics. The following eight metabolites were identified from the loading plots of multivariate analysis of the 13C-NMR spectra; naringin, neohesperidin, narirutin, synephrine, sucrose, α-glucose, β-glucose, and limonene. 13C-NMR spectra-based metabolic fingerprinting is a promising strategy for...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 30, 2018 Category: Drugs & Pharmacology Source Type: research

Optimization of an innovative vinylimidazole-based monolithic stationary phase and its use for pressured capillary electrochromatography
Publication date: Available online 28 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Adele Murauer, Rania Bakry, Gabriel Partl, Christian W. Huck, Markus GanzeraAbstractA novel polymer monolith based on the dicationic crosslinker 3,3’-(hexane-1,6-diyl)bis(1-vinylimidazolium) bromide, the monomer 1-vinylimidazole and a ternary porogen mixture (1-propanol, decan-1-ol and water) was developed and optimized for capillary electrochromatography. This aim was accomplished by adjusting the composition of individual constituents in the polymerization mixture and monitored based on several relev...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 30, 2018 Category: Drugs & Pharmacology Source Type: research

Urinary excretion studies of meldonium after multidose parenteral application
Publication date: Available online 28 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Guro Forsdahl, Biljana Jančić-Stojanović, Marija Anđelković, Nenad Dikić, Tomas Geisendorfer, Veronika Jeitler, Günter GmeinerAbstractMeldonium is a drug exhibiting cardioprotective and anti-ischemic effects. Due to its potential performance-enhancing benefit in sports, meldonium was added to the World Anti-Doping Agency list of prohibited substances in 2016. Since then, a high number of adverse analytical findings reported on meldonium has questioned meldonium`s detection time in urine. Hence, th...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 30, 2018 Category: Drugs & Pharmacology Source Type: research

Simultaneous determination of seven flavonoids, two phenolic acids and two cholesterines in Tanreqing injection by UHPLC-MS/MS
Publication date: Available online 28 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Cui Li, Chen Zang, Qixia Nie, Bo Yang, Baoxian Zhang, Shaofeng DuanAbstractA new ultra-high performance liquid chromatography combined with triple quadrupole mass spectrometry was developed to evaluate the quality of Tanreqing injection. Seven flavonoids (Rutin, Baicalin, Scutellarin, Chrysin-7-O-Beta-D-glucoronide, Oroxylin A-7-O-β-D-glucoronide, Wogonin, Luteolin-7-O-glucoside), two phenolic acids (Chlorogenic acid, Caffeic acid) and two cholesterines (Ursodeoxycholic acid, Chenodeoxycholic acid) in T...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 30, 2018 Category: Drugs & Pharmacology Source Type: research

Development of a novel HPLC method for the determination of the impurities in desonide cream and characterization of its impurities by 2D LC-IT-TOF MS
Publication date: Available online 27 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Jian Wang, Shuxian Zheng, Yu Xu, Haiyan Hu, Minji Shen, Lan TangAbstractA novel HPLC method for the determination of the impurities in desonide cream was established and validated for the further improvement of the official monograph in USP. Desonide was well resolved from the photodegradation impurity, which overlapped with desonide in USP method. The method was validated in accordance to the regulatory guidelines recommended by the International Conference on Harmonisation and this validation included spec...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 29, 2018 Category: Drugs & Pharmacology Source Type: research

A novel strategy to evaluate the quality of herbal products based on the chemical profiling, efficacy evaluation and pharmacokinetics
Publication date: Available online 26 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Yinghui Wen, Li He, Rui Peng, Yonghong Lin, Lina Zhao, Xiaoxiao Li, Liming Ye, Junyi YangAbstractThe purpose of this study was to establish a chemical profiling method to compare the chemical composition of herbal products by using extracts of Belamcandae Rhizoma(EBR) extracted with different polarity solvent as an example, and evaluate the quality of EBR based on the analysis of chemical profiling, efficacy evaluation and pharmacokinetics. As seen from the results of chemical profiling, the PCA and PLS-DA s...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 27, 2018 Category: Drugs & Pharmacology Source Type: research

DNA logic circuits based amplification system for quencher-free and highly sensitive detection of DNA and adenosine triphosphate
Publication date: Available online 27 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Yongjie Xu, Nana Geng, Xiang Zheng, Xiangrong Luo, Mingsong Wu, Hua ZhangAbstractWe fabricated a quencher-free and enzyme-free fluorescence detection system by employing the DNA logic circuits as signal amplifier and 2-aminopurine as signal indicator, and applied it to detect DNA and adenosine triphosphate (ATP). The assay system consisted of three hairpin probes with a sequestered 2-aminopurine molecule in each stem domain which was defined as inputs A, B and C of the logic operation. These three hairpin in...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 27, 2018 Category: Drugs & Pharmacology Source Type: research

Fast discrimination of the geographical origins of notoginseng by near-infrared spectroscopy and chemometrics
Publication date: Available online 27 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Hui Chen, Zan Lin, Chao TanAbstractNotoginseng is a type of highly valued Traditional Chinese medicine (TCM) due to its hemostatic and cardiovascular functions. Notoginseng of Yunnan in China usually commands a premium price and is often the subject of fraudulent practices. The feasibility of combining near-infrared (NIR) spectroscopy with chemometrics was investigated to discriminate notoginseng of different geographical origins. A total of 250 samples of four different provinces in China were collected and...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 27, 2018 Category: Drugs & Pharmacology Source Type: research

UPLC-Q-TOF/MS-based metabolic profiling comparison of four major bioactive components in normal and CKD rat plasma, urine and feces following oral administration of Cornus officinalis Sieb and Rehmannia glutinosa Libosch herb couple extract
In this study, UPLC-Q-TOF/MS technique combined with MetabolynxTM software, was developed and successfully applied for analysis of metabolic profiles of the bioactive components of the herb couple in normal and CKD rat biological samples. Main parent components of the herb couple extract such as loganin, morroniside and catalpol were absorbed into the blood circulation of the normal and CKD rats. Another parent component acteoside was almost completely degraded. Seventeen metabolites involved in the in vivo metabolism processes were tentatively identified. These metabolites indicated that loganin was mainly metabolized to ...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 26, 2018 Category: Drugs & Pharmacology Source Type: research

Pellet Patented Technology for Fast and Distinct Visual Detection of Cholinesterase Inhibitors in Liquids
Publication date: Available online 25 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Jakub Vysloužil, David Vetchý, Jiří Zeman, Oldřich Farsa, Aleš Franc, Jan Gajdziok, Jan Vysloužil, Katarína Ficeriová, Pavel Kulich, Zbyněk Kobliha, Vladimír PitschmannAbstractThe main objective of the presented research was to prepare an innovative carrier as a filler for detection tubes in the form of double-coated pellets with a very significant color transition during the detection of cholinesterase inhibitors such as nerve agents, organophosphorus or carbam...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 26, 2018 Category: Drugs & Pharmacology Source Type: research

Green and efficient extraction of four bioactive flavonoids from Pollen Typhae by ultrasound-assisted deep eutectic solvents extraction
In this study, a highly and efficient extraction technique for determination of four bioactive flavonoids from Pollen Typhae using a combination of ultrasound-assisted extraction and natural deep eutectic solvents (NADESs) was developed. A series of DESs containing various hydrogen bond acceptors combined with different hydrogen bond donors were synthesized and screened for high extraction efficiencies based on the flavonoids extraction yields. The extraction conditions including composition of DES, water content in DES, liquid-solid ratio, and extraction time were statistically optimized by single-factor experiment. As a ...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 26, 2018 Category: Drugs & Pharmacology Source Type: research

Metabolomics in chronic kidney disease: Strategies for extended metabolome coverage
Publication date: Available online 25 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Gagnebin Yoric, Boccard Julien, Ponte Belén, Rudaz SergeABSTRACTChronic kidney disease (CKD) is becoming a major public health issue as prevalence is increasing worldwide. It also represents a major challenge for the identification of new early biomarkers, understanding of biochemical mechanisms, patient monitoring and prognosis. Each metabolite contained in a biofluid or tissue may play a role as a signal or as a driver in the development or progression of the pathology. Therefore, metabolomics is a ...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 26, 2018 Category: Drugs & Pharmacology Source Type: research

Ultrahigh-performance liquid chromatography-tandem mass spectrometry (UHPLC–MS/MS) assay for simultaneous quantifications of CZ48, lactone-stabilized camptothecin, and camptothecin and their pharmacokinetic and biliary evaluations in rats
In this study, a UHPLC–MS/MS method was developed and validated to simultaneously quantify CZ48 and CPT in rat plasma and bile. Detection was performed using the API 3200 Q Trap triple quadrupole mass spectrometer in a positive ion mode. Chromatographic separation was achieved on Waters ACQUITY UPLC BEH Shield RP18 column with a gradient elution at a flow rate of 0.45 ml/min, using mobile phases of 0.1% acetic acid in water (A) and 0.1% acetic acid in acetonitrile (B). The method was linear at the concentration ranges of 0.98 (LLOQ) −1000 ng/ml of CZ48 and CPT in rat plasma and 3.9 (LLOQ) −1...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 25, 2018 Category: Drugs & Pharmacology Source Type: research

Developing a rapid semi-automated sample preparation with alkaline hydrolysis in a 96-well plate for quantification of 11-nor-Δ9-tetrahydrocannabinol-9-carboxylic acid in urine samples by UHPLC-MS/MS
Publication date: Available online 23 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Lill Dannevig Müller, Mimi Stokke OpdalAbstractA simple, sensitive, rapid and robust manual (in glass tubes) and semi-automatic method (in a 96-well plate format) for quantification of 11-nor-Δ9-tetrahydrocannabinol-9-carboxylic acid (THCA) in urine using a UHPLC-MS/MS have been developed. The methods involved hydrolysis with potassium hydroxide in a 96-well plate, followed by neutralization and dilution of the sample in one step with a stable solution of formic acid-methanol-acetonitrile-water (2...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 24, 2018 Category: Drugs & Pharmacology Source Type: research

Modified glassy carbon electrode with Polydopamine-multiwalled carbon nanotubes for simultaneous electrochemical determination of biocompounds in biological fluids
In this study, a simple modified glassy carbon electrode with Polydopamine-multiwalled carbon nanotubes (GCE/PDA-MWCNTs) is described for selective and sensitive simultaneous voltammetric determination of dopamine (DA), acetaminophen (AC), and xanthine (XN). A detailed investigation by field emission scanning electron microscopy, Fourier transform infrared spectroscopy and electrochemistry methods such as cyclic voltammetry (CV), electrochemical impedance spectroscopy, differential pulse voltammetry (DPV) and chronoamperometry are performed in order to elucidate the preparation process and properties of the GCE/ PDA-MWCNTs...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 24, 2018 Category: Drugs & Pharmacology Source Type: research

Quantitative structure-activity relationship for estrogenic flavonoids from Psoralea corylifolia
In conclusion, molecular docking can potentially be applied for predicting the receptor-binding properties of undescribed compounds based on their molecular structure.Graphical abstract (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - August 23, 2018 Category: Drugs & Pharmacology Source Type: research

Sensitive targeted methods for brain metabolomic studies in microdialysis samples
Publication date: Available online 23 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Jana Bongaerts, Dimitri De Bundel, Debby Mangelings, Ilse Smolders, Yvan Vander Heyden, Ann Van EeckhautAbstractIn vivo determination of brain mediators plays an important role in providing insight in how the brain functions. For this purpose, targeted metabolomics can be a very useful tool. Targeted metabolomics detects and measures certain known low-molecular-weight biomolecules involved in signaling pathways and biochemical processes in the central nervous system. Microdialysis is a powerful technique to ...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 23, 2018 Category: Drugs & Pharmacology Source Type: research

First inter-laboratory study of a Supercritical Fluid Chromatography method for the determination of pharmaceutical impurities
This study involved 19 participating laboratories from 4 continents and 9 different countries. It included 5 academic groups, 3 demonstration laboratories at analytical instrument companies, 10 pharmaceutical companies and 1 food company. In the initial analysis of the study results, consistencies within- and between-laboratories were deeply examined. In the subsequent analysis, the method reproducibility was estimated taking into account variances in replicates, between-days and between-laboratories. The results obtained were compared with the literature values for liquid chromatography (LC) in the context of impurities d...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 23, 2018 Category: Drugs & Pharmacology Source Type: research

Behavior of pesticides and their metabolites in traditional Chinese medicine Paeoniae Radix Alba during processing and associated health risk
This study main investigated the dissipation and metabolism of pesticides in Chinese medicine Paeoniae Radix Alba during processing, and the calculated pesticide PFs were also used to assess the risk of dietary exposure. For analyze samples, a simple and high-throughput multiresidue pesticide analysis method was developed and validated for pesticides and their metabolites in P. Radix. based on QuEChERS procedure combined with HPLC-MS/MS. Recoveries at three concentration levels were within 61.37%–117.82% with an associated precision RSD 
Source: Journal of Pharmaceutical and Biomedical Analysis - August 21, 2018 Category: Drugs & Pharmacology Source Type: research

Liquid chromatographic techniques in betacyanin isomers separation from Gomphrena globosa L. flowers for the determination of their antimicrobial activities
Publication date: Available online 15 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Aneta Spórna-Kucab, Katarzyna Bernaś, Agnieszka Grzegorczyk, Anna Malm, Krystyna Skalicka-Woźniak, Sławomir WybraniecAbstractHigh-speed counter-current chromatography (HSCCC) combined with HPLC were established to purify, for the first time, betacyanin isomers from Gomphrena globosa L. flowers. The mixture of tert-butyl methyl ether – butanol – methanol - water TBME-BuOH-MeOH-H2O (1.0% heptafluorobutyric acid HFBA) 2:2:1:5 (v/v/v/v) in reversed phase mode was used for preliminary purifi...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 21, 2018 Category: Drugs & Pharmacology Source Type: research

Dispersive magnetic solid phase extraction exploiting magnetic graphene nanocomposite coupled with UHPLC-PDA for simultaneous determination of NSAIDs in human plasma and urine
Publication date: Available online 16 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Vincenzo Ferrone, Maura Carlucci, Valeria Ettorre, Roberto Cotellese, Paola Palumbo, Antonella Fontana, Gabriella Siani, Giuseppe CarlucciAbstractA novel, rapid, simple graphene/Fe3O4 based dispersive magnetic solid phase extraction was developed for the simultaneous separation/preconcentration and determination of non steroidal anti-inflammatory drugs with ultra high performance liquid chromatography coupled with photodiode array detection. Several parameters influencing the extraction efficiency of the inv...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 21, 2018 Category: Drugs & Pharmacology Source Type: research

A Rapid, Simple, and High-throughput UPLC-MS/MS Method for Simultaneous Determination of Bioactive Constituents in Salvia Miltiorrhiza with Positive/Negative Ionization Switching
Publication date: Available online 16 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Hung-Yu Lin, Ting-Sian Lin, Han-Ju Chien, Yu-Min Juang, Chao-Jung Chen, Chang-Sheng Wang, Chien-Chen LaiAbstractThe dried root of Danshen (Salvia miltiorrhiza) is a Chinese medicine that has been used in traditional treatments for more than 2,000 years in Asia. It has also been used to treat cardiovascular diseases for decades. The goal of this study was to develop a new, fast, and simple ultrahigh-performance liquid chromatography tandem mass spectrometry (UPLC–MS/MS) analytical method for the analysi...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 21, 2018 Category: Drugs & Pharmacology Source Type: research

Precipitation behavior of pioglitazone on the particle surface of hydrochloride salt in biorelevant media
Publication date: Available online 16 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Taiga Uekusa, Kiyohiko SuganoAbstractThe purpose of the present study was to investigate the precipitation of a drug on the particle surface of its salt in biorelevant media. Pioglitazone (PIO, weak base, pKa = 5.8) was used as a model drug. The crystal particles of PIO hydrochloride (PIO HCl) were immobilized between two slide glasses. A biorelevant medium was penetrated between the slide glasses under a polarized light microscope. Crystalline precipitates appeared on the surface of PIO HCl wi...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 21, 2018 Category: Drugs & Pharmacology Source Type: research

Recent development and trends in sample extraction and preparation for mass spectrometric analysis of nucleotides, nucleosides, and proteins
Publication date: Available online 17 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Tadashi Hayama, Kaname OhyamaAbstractThis review describes the recent developments in sample extraction and preparation techniques for mass spectrometric analysis of nucleotides, nucleosides, and proteins. Unique materials and techniques have been developed for highly selective extraction of nucleotides and nucleosides by solid-phase extraction strategies using various affinities. However, for proteins, the analysis of small-scale sections of diseased tissues (formalin-fixed, paraffin-embedded tissues) and t...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 21, 2018 Category: Drugs & Pharmacology Source Type: research

Modified glassy carbon electrode with Polydopamine - multiwalled carbon nanotubes for simultaneous electrochemical determination of biocompounds in biological fluids
In this study, a simple modified glassy carbon electrode with Polydopamine-multiwalled carbon nanotubes (GCE/PDA-MWCNTs) is described for selective and sensitive simultaneous voltammetric determination of dopamine (DA), acetaminophen (AC), and xanthine (XN). A detailed investigation by field emission scanning electron microscopy, Fourier transform infrared spectroscopy and electrochemistry methods such as cyclic voltammetry (CV), electrochemical impedance spectroscopy, differential pulse voltammetry (DPV) and chronoamperometry are performed in order to elucidate the preparation process and properties of the GCE/ PDA-MWCNTs...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 21, 2018 Category: Drugs & Pharmacology Source Type: research

LC–MS/MS assay for the determination of a novel D-peptide antagonist of CXCR4 in rat plasma and its application to a preclinical pharmacokinetic study
Publication date: Available online 17 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Yinsong Zhu, Shu Yang, Juan Wang, Yujia Mao, Yan Xu, Jing An, Ziwei HuangAbstractDV1 is a potent and selective D-peptide antagonist of CXCR4 and being developed as a novel drug candidate molecule. For preclinical pharmacokinetic study of DV1, we established an efficient and reliable liquid chromatography coupled to tandem mass spectrometric (LC–MS/MS) method for the assay of DV1 in rat plasma. Plasma samples were acidified by formic acid and then their protein content precipitated by acetonitrile. Samp...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 21, 2018 Category: Drugs & Pharmacology Source Type: research

Characterization of a new illicit phosphodiesterase-type-5 inhibitor identified in the softgel shell of a dietary supplement
Publication date: Available online 18 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Takahiro Doi, Kazunaga Takahashi, Midori Yamazaki, Akiko Asada, Akihiro Takeda, Kyohei Kiyota, Takaomi Tagami, Yoshiyuki Sawabe, Tetsuo YamanoAbstractA new sildenafil analog has been identified in the softgel shell of a dietary supplement. The compound was investigated by UV spectroscopy and high-resolution MS analysis, leading to the proposed structure 1-methyl-5-{5-[2-(4-methylpiperazin-1-yl)acetyl]-2-propoxyphenyl}-3-propyl-1,6-dihydro-7H-pyrazolo[4,3-d]pyrimidin-7-one. A synthetic reference compound with...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 21, 2018 Category: Drugs & Pharmacology Source Type: research

A Functional In Vitro Assay for Screening Inhibitors of STAT5B Phosphorylation
Publication date: Available online 18 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Elvin D. de Araujo, Pimyupa Manaswiyoungkul, Fettah Erodgan, Abdul K. Qadree, Diana Sina, Gary Tin, Krimo Toutah, Karen Yuen, Patrick T. GunningAbstractInhibition of STAT phosphorylation is recognized as a viable therapeutic strategy for disrupting tumorigenesis. Constitutive STAT phosphorylation is found with high frequency in a number of primary tumor types, while non-cancer cells exhibit low basal activity, providing an exploitable therapeutic window. STAT activation involves phosphorylation of the SH2 do...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 21, 2018 Category: Drugs & Pharmacology Source Type: research

In vitro binding interaction of atorvastatin with calf thymus DNA: multispectroscopic, gel electrophoresis and molecular docking studies
Publication date: Available online 18 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Zeinab Mirzaei-KalarAbstractThe interaction of atorvastatin with calf thymus DNA (CT-DNA) was investigated in vitro under simulated physiological conditions by using absorption and emission spectroscopy, viscosity measurements, gel electrophoresis and molecular docking studies. Analysis of UV-Visible absorbance spectra indicates the formation of complex between atorvastatin and CT-DNA, and obtained binding constant (Kb = 8.2×104 L. mol-1) is comparable to groove binder drugs. Sligh...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 21, 2018 Category: Drugs & Pharmacology Source Type: research

A new method for the determination of total and released docetaxel from docetaxel-entrapped core-crosslinked polymeric micelles (CriPec®) by LC-MS/MS and its clinical application in plasma and tissues in patients with various tumours
In conclusion the newly developed assay met the required standards for validation and was applied successfully to support pharmacokinetic analysis in both serum and tissue in patients treated with Cripec®. (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - August 21, 2018 Category: Drugs & Pharmacology Source Type: research

Identification of naringin metabolites mediated by human intestinal microbes with stable isotope-labeling method and UFLC-Q-TOF-MS/MS
In this study, we aim of systematically investigating the biotransformation of naringin mediated by human intestinal microbes through applying stable isotope-labeling method. [2',3',5',6'-D4]naringin was synthesized and incubated anaerobically with human gut microbes. A total of 13 microbial metabolites were detected and identified by UFLC-Q-TOF-MS/MS, among which 5 were reported for the first time. Furthermore, the proposed metabolic pathway revealed that naringin went through extensive phase I metabolism in human intestinal microbes. Of note, diverse metabolic profiles of naringin among human participants were obtained, ...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 21, 2018 Category: Drugs & Pharmacology Source Type: research

Bioanalytical liquid chromatography-tandem mass spectrometric assay for the quantification of the ALK inhibitors alectinib, brigatinib and lorlatinib in plasma and mouse tissue homogenates
Publication date: Available online 19 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Rolf W. Sparidans, Wenlong Li, Alfred H. Schinkel, Jan H.M. Schellens, Jos H. BeijnenAbstractSeveral second and third generation ALK inhibitors have been introduced in recent years. A bioanalytical assay for simultaneous quantification of alectinib, brigatinib, and lorlatinib was developed and validated for human plasma. The method was also partially validated for diluted mouse plasma and tissue homogenates of brain, liver, kidney, and spleen. Samples (40 µl) were pretreated in a 96-well plate by pro...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 21, 2018 Category: Drugs & Pharmacology Source Type: research

Falsification of biotechnology drugs: Current dangers and/or future disasters?
Publication date: Available online 20 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Steven Janvier, Bart De Spiegeleer, Celine Vanhee, Eric Deconinck (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - August 21, 2018 Category: Drugs & Pharmacology Source Type: research

Use of thermal and non thermal techniques for assessing compatibility between mirtazapine and solid lipids
Publication date: Available online 20 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Randeep Kaur, V.R. SinhaAbstractThe present investigation was aimed at the evaluation of possible interactions between mirtazapine and selected solid lipids that are commonly used to develop solid lipid nanoparticles (SLNs) and nanostructured lipidic carriers (NLCs). The solids lipids explored were palmitic acid, stearic acid, glycerylmonostearate, cutina CPPH, sterotex NF, gelucire 50/13, hydrogenated castor oil and compritol 888 ATO. The techniques used were Differential Scanning Calorimetry (DSC), Fourier...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 21, 2018 Category: Drugs & Pharmacology Source Type: research

Nontargeted metabolomics approach to determine metabolites profile and antioxidant study of Tropical Almond (Terminalia catappa L.) fruit peels using GC-QTOF-MS and LC-QTOF-MS
The objective of the present study was to identify the metabolome pattern and study the biological efficacy of Almond (Terminalia catappa L.) peels. In the present study, metabolite profiling was carried out using GC-QTOF-MS and LC-QTOF-MS techniques, from the potent extract showed highest antioxidant efficacy. Antioxidant efficacy of the various extracts derived by six different extraction methods was assessed by different assays viz. 2,2-diphenyl-1-picrylhydrazyl, superoxide, 2,2′-Azino-bis-(3-ethyl)benzothiazoline)-6-sulfonic acid diammonium salt radical cation inhibition assays and ferric reducing antioxidant pow...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 21, 2018 Category: Drugs & Pharmacology Source Type: research

Novel electrophoretic acetonitrile-based stacking for sensitive monitoring of the antiepileptic drug perampanel in human serum
Publication date: 25 October 2018Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 160Author(s): Petr Tůma, Miroslava Bursová, Blanka Sommerová, Rachel Horsley, Radomír Čabala, Tomáš HložekAbstractPerampanel is a novel antiepileptic drug used in paediatric patients. Existing methods that determine serum perampanel are of limited practicability. We developed a novel capillary electrophoresis (CE) method using a new version of acetonitrile stacking for on-line sample pre-concentration, and fluorescence detection (FD). CE separations were performed in a fused-silica capill...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 17, 2018 Category: Drugs & Pharmacology Source Type: research

Rapid authentication and differentiation of herbal medicine using 1H NMR fingerprints coupled with chemometrics
In conclusion, the developed method is an accurate, sensitive and rapid authentication of Polygoni Multiflori Radix, which could be a useful tool for rapid authentication and quality control of herbal medicines. (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - August 14, 2018 Category: Drugs & Pharmacology Source Type: research

Paper-based analytical devices prepared with polycaprolactone printing and their application in the activity determination of mulberry extracts
Publication date: Available online 11 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Guo Shangxin, Shao Jingyuan, Gong XingchuAbstractA new method for preparing paper-based analytical devices (PADs) with the assistance of 3D printing technology was presented. Polycaprolactone was used as the 3D printing material to form hydrophobic barriers. First, polycaprolactone was 3D printed on filter paper. The polycaprolactone-modified paper was then prepared by heating. Experiments on α-glucosidase inhibition activity were carried out in the hydrophilic circles on the modified paper. Color data...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 12, 2018 Category: Drugs & Pharmacology Source Type: research