Analysis of chlorhexidine gluconate in skin using tape stripping and ultrahigh-performance liquid chromatography-tandem mass spectrometry
Publication date: Available online 6 February 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Xueheng Zhao, Joshua K. Schaffzin, Jennifer Carson, Andrea Ankrum, Edward Dobrzykowski, David Haslam, Christopher Dandoy, Kenneth D.R. SetchellAbstractBackgroundTopical chlorhexidine gluconate (CHG) is used widely to reduce healthcare-associated infection. The optimal therapeutic dose for maximum efficacy and reduced toxicity is unclear, in part because of the lack of analytical methods to monitor CHG levels in skin. A novel method was developed to accurately measure CHG levels in skin after topical applica...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 7, 2020 Category: Drugs & Pharmacology Source Type: research

Determination of Heavy Metals in Cancerous and Healthy Colon Tissues using Laser Induced Breakdown Spectroscopy and its Cross-Validation with ICP-AES Method
Publication date: Available online 7 February 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): M.A. Gondal, R.K. Aldakheel, M.A. Almessiere, M.M. Nasr, J.A. Almusairii, B. GondalAbstractCertain types of cancer deaths are widespread due to the diagnostic complexities at the early stages. Recent studies revealed that the bio-accumulated heavy metals (act as pro-carcinogens) in the human colon cause strong interactions with the nuclear proteins, leading to the DNA damages and repair processes inhibition, resulting in the cancers. Most of the conventional detection techniques suffer from the insensitiven...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 7, 2020 Category: Drugs & Pharmacology Source Type: research

Impurity profiling of l-aspartic acid and glycine using high-performance liquid chromatography coupled with charged aerosol and ultraviolet detection
Publication date: Available online 5 February 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Ruben Pawellek, Klaus Schilling, Ulrike HolzgrabeAbstractFor the compendial related substances test of L-aspartic acid (Asp) and glycine (Gly), two separate reversed-phase ion-pair high-performance liquid chromatography methods coupled with charged aerosol and ultraviolet detection were developed. Separation of all putative impurities, in particular of the related carboxylic and amino acids, was achieved using volatile perfluorocarboxylic acids as ion-pairing reagents on a polar embedded C18 stationary phas...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 7, 2020 Category: Drugs & Pharmacology Source Type: research

Synthesis and characterization of oxitropium bromide related substances and novel stability indicating HPLC methods
Publication date: Available online 5 February 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Esen Bellur Atici, Çağan Ağtaş, Yücel Yazar, Nurten RıdvanoğluAbstractThe European Pharmacopoeia (Ph. Eur.) described two separate HPLC methods for determination of organic impurities in oxitropium bromide, a synthetic anticholinergic agent used by inhalation in the treatment of asthma and other bronchial disorders, and a potentiometric titration assay method which is not a stability indicating method. During synthetic process development and analytical studies of oxitropium; besides known ...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 7, 2020 Category: Drugs & Pharmacology Source Type: research

A stability-indicating LC–MS/MS method optimization for Pemetrexed through design of experiments: Identification and characterization of major oxidative degradation product
Publication date: Available online 5 February 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): S.T Narenderan, J Ramesh, B Babu, S.N MeyyanathanAbstractA stability-indicating high-performance liquid chromatography method for Pemetrexed bulk was developed and validated as per the ICH guidelines by employing design of experiments methodology equipped with Box–Behnken design. The separation of Pemetrexed and its oxidative degradation product was achieved in isocratic elution mode with Zorbax C18 column (150 mm x 4.6 mm x5 µm) using 10 mM ammonium ace...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 7, 2020 Category: Drugs & Pharmacology Source Type: research

Reversed phase UPLC/APCI-MS determination of Vitamin K1 and menaquinone-4 in human plasma: application to a clinical study
Publication date: Available online 4 February 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Yuning Zhang, Yashpal S. Chhonker, Veenu Bala, Alex Hagg, Linda G. Snetselaar, Terry L. Wahls, Daryl J. MurryAbstractA rapid and sensitive liquid chromatographic-tandem mass spectrometric method was developed and validated to simultaneously quantitate vitamin K1 (PK) and vitamin K2 (MK-4) concentrations in human plasma to evaluate nutritional interventions. Charcoal-stripped human plasma was used for standard and calibration preparation with vitamin E-d6 as the internal standard. Patient plasma samples were...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 5, 2020 Category: Drugs & Pharmacology Source Type: research

Qualitative and quantitative determination of anaprazole and its major metabolites in human plasma
Publication date: Available online 4 February 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Chongzhuang Tang, Liang Li, Xifeng Ma, Jin Wang, Bo Chen, Xiaojian Dai, Yifan Zhang, Xiaoyan ChenAbstractAnaprazole is a novel proton pump inhibitor under development for the treatment of gastric and duodenal ulcers. In the present study, an ultra-performance liquid chromatography-ultraviolet detector/quadrupole time-of-flight mass spectrometry method was developed for the metabolic profiling of human plasma after an oral administration of 40 mg anaprazole. The principal metabolic pathways were ident...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 5, 2020 Category: Drugs & Pharmacology Source Type: research

Correlation study between the pharmacokinetics of seven main active ingredients of Mahuang decoction and its pharmacodynamics in asthmatic rats
Publication date: Available online 3 February 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Pengcheng Huang, Yinghong Tang, Chang Li, Huifen Zhou, Li Yu, Haitong Wan, Yu HeAbstractThe aim of this study was to investigate the pharmacokinetics and pharmacodynamics of seven main active components of Mahuang decoction (MHD) and its time-concentration-effect relationship. The asthmatic rat model was established by the method of ovalbumin (OVA) sensttization. The plasma concentrations of ephedrine, pseudoephedrine, methylephedrine, amygdalin, liquiritin, cinnamic acid, glycyrrhizic acid in asthmatic mod...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 4, 2020 Category: Drugs & Pharmacology Source Type: research

Elimination pharmacokinetics of sphaeralcic acid, tomentin and scopoletin mixture from a standardized fraction of Sphaeralcea angustifolia (Cav.) G. Don orally administered
The objective of this work was to determine the pharmacokinetic constants of urinary and fecal elimination in mice of the anti-arthritic compounds, after oral administration (400 mg / kg) of a standardized active fraction (SaTES) of S. angustifolia. It was established that the coumarin mixture (scopoletin and tomentin) were eliminated by the urine; while, sphaeralcic acid was mainly eliminated by fecal path, following both a non-compartmental behavior. No products derived from their biotransformation were detected.Graphical abstract (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - February 4, 2020 Category: Drugs & Pharmacology Source Type: research

NMR-derived targeted serum metabolic biomarkers appraisal of bladder cancer: A pre- and post-operative evaluation
This study was conducted on 160 sera samples involving HC (n = 52), pre-operative (n = 55) and post-operative (n = 53) BC cases. 1H nuclear magnetic resonance (NMR) spectroscopy was used to generate serum metabolic profiles and to gauge aberrantly expressed metabolites. The targeted metabolomic approach revealed that the expression levels of these signature metabolites were progressively and significantly decreased in post-operative follow-up at the interval of 30, 60, and 90 days compared to pre-operative BC sera samples and were maintained at HC levels. Serum metabolic biomarkers...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 3, 2020 Category: Drugs & Pharmacology Source Type: research

Development and validation of a UPLC-MS/MS method for simultaneous quantification of levofloxacin, ciprofloxacin, moxifloxacin and rifampicin in human plasma: application to the therapeutic drug monitoring in osteoarticular infections
ConclusionA simple, rapid, and selective liquid chromatography-tandem mass spectrometry method was developed and validated for the simultaneous quantification of levofloxacin, ciprofloxacin, moxifloxacin and rifampicin in human plasma.Graphical abstract (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - February 3, 2020 Category: Drugs & Pharmacology Source Type: research

Pharmacokinetics, tissue distribution, and safety evaluation of a ligustilide derivative (LIGc)
Publication date: Available online 31 January 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Yanxia Zhang, Yaming Zhang, Yanming Han, Ye Tian, Pengcheng Wu, Aiyi Xin, Xiaoning Wei, Yanbin Shi, Zhenchang Zhang, Gang Su, Yanping Shi, Junxi LiuAbstractLigustilide (LIG) is the main active ingredient of Angelica sinensis (Oliv.) Diels and has a neuroprotective effect against ischemic stroke. However, owing to its multi-conjugated unstable structure, the compound has poor drug-forming properties. Therefore, we synthesized highly stable colorless needle crystals (known as ligusticum cycloprolactam, LIGc) ...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 1, 2020 Category: Drugs & Pharmacology Source Type: research

Active vitamin D and vitamin D analogs stimulate fibroblast growth factor 23 production in osteocyte-like cells via the vitamin D receptor
Publication date: Available online 31 January 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Mitsuru Yashiro, Masaki Ohya, Toru Mima, Yuri Nakashima, Kazuki Kawakami, Shuto Yamamoto, Sou Kobayashi, Takurou Yano, Yusuke Tanaka, Tomohiro Sonou, Kouichi Tatsuta, Shigeo Negi, Takashi ShigematsuAbstractOsteocytes play an important role in the regulation of serum phosphorus by producing fibroblast growth factor 23 (FGF23). FGF23 production is stimulated by 1α,25-dihydroxyvitamin D in osteocytes. However, it is unclear whether vitamin D induces FGF23 production in osteocytes directly. Therefore, we ...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 1, 2020 Category: Drugs & Pharmacology Source Type: research

Production and purification of anti-tubercular and anticancer protein from Staphylococcus hominis under mild stress condition of Mentha piperita L.
In conclusion, protein purified from strain MANF2 under mild stress of M. piperita L can certainly be implied as efficacious anti-tubercular and anticancer agents in future. (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - February 1, 2020 Category: Drugs & Pharmacology Source Type: research

Combination of HDX-MS and in silico modeling to study enzymatic reactivity and stereo-selectivity at different solvent conditions
Publication date: Available online 1 February 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Alexey A. Makarov, Roxana E. Iacob, Gregory F. Pirrone, Agustina Rodriguez-Granillo, Leo Joyce, Ian Mangion, Jeffrey C. Moore, Edward C. Sherer, John R. EngenAbstractThe higher-order structure of a protein defines its function, and protein structural dynamics are often essential for protein binding and enzyme catalysis. Methods for protein characterization in solution are continuously being developed to understand and explore protein conformational changes with regards to function and activity. The goal of ...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 1, 2020 Category: Drugs & Pharmacology Source Type: research

A method for detecting tumor cells derived from colorectal cancer by targeting cell surface glycosylation with affinity capillary electrophoresis
Publication date: Available online 30 January 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Tetsushi Yamamoto, Kanta Sato, Shinpei Wakahara, Kuniko Mitamura, Atsushi TagaAbstractCirculating tumor cells (CTCs) are involved in metastasis; thus, one of the most important approaches for identifying metastatic cancer is to detect CTCs in blood. In the present study, we examined whether directly analyzing cells with capillary electrophoresis (CE) could distinguish cancer cells from normal cells, based on differences in cell surface glycosylation. We compared human colorectal cancer (CRC) cell lines to a...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 31, 2020 Category: Drugs & Pharmacology Source Type: research

Aspects of Matrix and Analyte Effects in Clinical Pharmacokinetic Sample Analyses using LC-ESI/MS/MS – Two Case Examples
Publication date: Available online 30 January 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Guohua An, Thanh Bach, Inas Abdallah, Demet NalbantAbstractThe increasing focus on high throughput sample analysis has led to the common practice of using simplest sample preparation method possible (i.e. protein precipitation) and shortest sample run-time possible. This means that there will be two aspects of compromise: the first compromise is made between sample cleanliness and sample preparation speed since protein precipitation does not provide very clean final extract; the second compromise is made be...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 30, 2020 Category: Drugs & Pharmacology Source Type: research

High Performance Liquid Chromatography-Electrospray Ionization Mass Spectrometric (LC-ESI-MS) Methodology for Analysis of Amino Acid Energy Substrates in Microwave-Fixed Microdissected Brain Tissue
Publication date: Available online 28 January 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Khaggeswar Bhehmanapally, Mostafa M.H. Ibrahim, karen P.B. BriskiAbstractHypoglycemia deprives the brain of its primary energy source glucose. Reductions in whole-brain amino acid energy substrate levels suggest that these non-glucose fuels may be metabolized during glucose shortage. Recurring hypoglycemia can cause mal-adaptive impairment of glucose counter-regulation; yet, it is unclear if amplified reliance upon alternative metabolic substrates impedes detection of continuing neuro-glucopenia. This resea...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 29, 2020 Category: Drugs & Pharmacology Source Type: research

Preparation and application of teicoplanin functionalized polymeric monolith for enantioseparation of chiral drugs
Publication date: Available online 27 January 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Rongying Luo, Hai Han, Jia Liu, Dongsheng Xu, Qiqin Wang, Salvatore Fanali, Zhengjin JiangAbstractA novel chiral stationary phase (CSP), based on a monolithic organic polymer chemically modified with teicoplanin, was fabricated within a 100 μm I.D. fused silica capillary. The teicoplanin was firstly derivatized with 2-isocyanatoethyl methacrylate (ICNEML) and then thermally co-polymerized with the crosslinker ethylene dimethacrylate (EDMA) in presence of porogens (methanol and dimethylsulfoxide). ...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 28, 2020 Category: Drugs & Pharmacology Source Type: research

Analytical Artifacts in characterization of recombinant monoclonal antibody therapeutics
Publication date: Available online 25 January 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Dongdong Wang, Christine Nowak, Bruce Mason, Amit Katiyar, Hongcheng LiuAbstractA battery of analytical methods is used to analyze recombinant monoclonal antibodies for lot release to ensure consistent product quality, safety, and efficacy. Additionally, state-of-the-art analytical methods have been used to thoroughly characterize various post-translational modifications and degradation pathways of those molecules. Scientifically sound and robust analytical methods are essential to providing reliable result...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 26, 2020 Category: Drugs & Pharmacology Source Type: research

A new validated HPLC-UV method for therapeutic monitoring of daptomycin in comparison with reference Mass Spectrometry
In conclusion, the present method demonstrated to be reliable and suitable for daptomycin TDM in clinical routine. (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - January 26, 2020 Category: Drugs & Pharmacology Source Type: research

Automated middle-up approach for the characterization of biotherapeutic products by combining on-line hinge-specific digestion with RPLC-HRMS analysis
This study describes, for the first time, the development of an automated liquid chromatography-mass spectrometry (LC-MS) workflow comprised of an immobilized IdeS-HPLC column for on-line sample digestion, followed by a reversed-phase liquid chromatography (RPLC) for protein subunit separation, and a high-resolution MS for molecular weight analysis. A proof of concept study was described here for the characterization of a therapeutic mAb and a bsAb. For the mAb, this automated workflow enabled rapid characterization of post-translational modifications (PTMs) such as N-glycosylation, glycation and N-terminal lysine. For the...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 26, 2020 Category: Drugs & Pharmacology Source Type: research

Synthetic NRG-1 functionalized DNA nanospindels towards HER2/neu targets for in vitro anti-cancer activity assessment against breast cancer MCF-7 cells
Publication date: Available online 25 January 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Mirza Muhammad Faran Ashraf Baig, Wing-Fu Lai, Reyaj Mikrani, Mehreen Jabeen, Muhammad Naveed, Muhammad Abbas, Muhammad Asim Farooq, Anam Ahsan, Said Abasse Kassim, Ghulam Jilany Khan, Muhammad Tayyab AnsariAbstractDNA based nano-carriers synthesized from short circular scaffolds (circular DNA nanotechnology) attains stiffer topology for ligand functionalization (neuregulin-1/NRG-1 ligand) and biological applications (targeted drug delivery). Daunorubicin (DR) is a hydrophobic chemical that requires robust ...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 26, 2020 Category: Drugs & Pharmacology Source Type: research

Discovery and validation of peptide biomarkers for discrimination of Dendrobium species by label-free proteomics and chemometrics
This study committed to discover specific peptides biomarkers for the authentication of D. officinale from the other two Dendrobium species using label-free proteomics by nanoLC LTQ Orbitrap mass spectrometry. Multivariate statistical analysis was applied to visualize the difference between the three Dendrobium species. As a result, 29 peptides among a total of 343 measurable peptides were selected to be potential biomarkers for the classification of these Dendrobium species. The validation of the representative peptide biomarkers was carried out by the synthesized peptides and 3 peptide biomarkers were found significant f...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 25, 2020 Category: Drugs & Pharmacology Source Type: research

Plasma mevalonic acid exposure as a pharmacodynamic biomarker of fluvastatin/atorvastatin in healthy volunteers
In conclusion, the data show that the plasma exposure of MVL represents a reliable pharmacodynamic parameter for PK-PD (pharmacokinetic-pharmacodynamic) studies of fluvastatin in multiple doses and atorvastatin in a single dose. (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - January 25, 2020 Category: Drugs & Pharmacology Source Type: research

Opportunities and obstacles for microsampling techniques in bioanalysis: Special focus on DBS and VAMS
Publication date: Available online 25 January 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Vaishali Londhe, Madhura RajadhyakshaAbstractMicrosampling, a reduced volume (
Source: Journal of Pharmaceutical and Biomedical Analysis - January 25, 2020 Category: Drugs & Pharmacology Source Type: research

Combination of intact, middle-up and bottom-up levels to characterize 7 therapeutic monoclonal antibodies by capillary electrophoresis – mass spectrometry
In this study, 7 worldwide health authorities approved mAbs have been analyzed to get information about their charge heterogeneity, the identification of post translational modifications (PTMs), their location and relative quantitation. Intact mAbs isoforms have been partially separated in less than 12 minutes and enabled to have a global illustration of mAbs heterogeneity and high masses PTMs characterization notably major N-glycosylation forms. Particularly, 2X-glycosylated and 1X-glycosylated forms have been partially separated. To deepen characterize PTMs carried by the backbone structure, advanced investigation...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 25, 2020 Category: Drugs & Pharmacology Source Type: research

Evaluation of a sensitive GC-MS method to detect polysorbate 80 in vaccine preparation
Publication date: Available online 23 January 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Niraj Shende, Asha Mallya (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - January 25, 2020 Category: Drugs & Pharmacology Source Type: research

Multivariate Quantitative Analysis of Quality Trend Based on Non-volatile Characteristic Components in different Panax notoginseng samples using HPLC
Publication date: Available online 23 January 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Chao Li, Yunhua Qin, Qianxu Yang, Junheng You, Zhihua Liu, Jingmei Han, E’xian Li, Chengming ZhangAbstractIn order to identify real and fake Panax notoginseng samples, the high performance liquid chromatography (HPLC) was used to analyze P. notoginseng samples of non-volatile characteristic components in P. notoginseng powder samples with 10%, 30%, 50% ratio, combined with principal component analysis - mahalanobis distance (PCA-MD). The results showed that: (1) The PCA analysis showed that mahalanobi...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 25, 2020 Category: Drugs & Pharmacology Source Type: research

Editorial Board
Publication date: 20 February 2020Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 180Author(s): (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - January 25, 2020 Category: Drugs & Pharmacology Source Type: research

Development of an ADP-Ribosylation Assay for residual toxicity in C. difficile Binary Toxin CDTa using Automated Capillary Western Blot
Publication date: Available online 23 January 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Richard R. Rustandi, Melissa HammAbstractCDTa, an actin ADP-ribosylation transferase, is a binary toxin produced by the bacterium Clostridium difficile which is commonly associated with the hypervirulent strain present in Clostridium difficile infections. The mutated form of CDTa, 4mCDTa, is one of the components in the tetravalent Clostridium difficile vaccine in which the residual toxicity of the ADP-ribosylation activity needs to be monitored for safety reasons. There are several ADP- ribosylation activi...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 23, 2020 Category: Drugs & Pharmacology Source Type: research

Simultaneous determination of carvacrol and thymol in bee pollen by using a simple and efficient solvent extraction method and gas chromatography-mass spectrometry
Publication date: Available online 23 January 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Ana M. Ares, María J. Nozal, José L. Bernal, José BernalAbstractA novel method is proposed to determine residues of carvacrol and thymol in bee pollen by means of gas chromatography coupled to mass spectrometry. This is an efficient and simple sample treatment (with average analyte recoveries between 90% and 104%) involving solvent extraction with hexane followed by evaporation. There is no need for any additional clean-up step, as the matrix did not affect determination of mass spectro...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 23, 2020 Category: Drugs & Pharmacology Source Type: research

Simultaneous quantification of thirty polyphenols in blackthorn flowers and dry extracts prepared thereof: HPLC-PDA method development and validation for quality control
Publication date: Available online 21 January 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Anna Marchelak, Monika Anna Olszewska, Aleksandra OwczarekAbstractThe paper presents development and validation of a RP-HPLC-PDA method for quantification of 30 phenolic constituents of the blackthorn (Prunus spinosa L.) flower, a traditional European herbal medicine with a unique and complex composition. The target analytes were selected from over 50 active compounds present in the investigated plant material, and their separation was optimized on a C18 Ascentis Express fused-core column (2.7 μm, 150 mm...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 22, 2020 Category: Drugs & Pharmacology Source Type: research

Development and Validation of a New IgE-Tolerant ELISA Method for Quantifying Serum Concentration of Omalizumab
ConclusionsIn conclusion, by dissociating IgE-omalizumab complex, the authors proposed for the first time a new validated IgE-tolerant ELISA assay to determine omalizumab concentration in serum samples.Graphical abstract (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - January 22, 2020 Category: Drugs & Pharmacology Source Type: research

Headspace gas chromatographic method for antimicrobial screening: minimum inhibitory concentration determination
Publication date: Available online 21 January 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Menghui Li, Chunyun Zhang, Guilin Chen, Lutfun Nahar, Satyajit D. Sarker, Mingquan GuoAbstractThe headspace gas chromatographic method has been widely used to detect volatile metabolites to reflect the growth state of microorganisms, however, it has never been used for the determination of the minimum inhibitory concentration in antibacterial drugs. This paper reports a new method for evaluating the antimicrobial activity of drugs by measuring the amount of CO2 produced by bacterial metabolism after treatme...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 22, 2020 Category: Drugs & Pharmacology Source Type: research

Characterization of therapeutic oligonucleotides by liquid chromatography
Publication date: Available online 20 January 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Alexandre Goyon, Peter Yehl, Kelly ZhangABSTRACTMarketed therapies in the pharmaceutical landscape are rapidly evolving and getting more diverse. Small molecule medicines have dominated in the past while antibodies have grown dramatically in recent years. However, the failure of traditional small and large molecules in accessing certain targets has led to increased R&D efforts to develop alternative modalities. Therapeutic oligonucleotides (ONs) can accurately be directed against their ribonucleic acid ...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 20, 2020 Category: Drugs & Pharmacology Source Type: research

HPLC-MS/MS method for the simultaneous quantification of dolutegravir, elvitegravir, rilpivirine, darunavir, ritonavir, raltegravir and raltegravir-β-D-glucuronide in human plasma
Publication date: Available online 18 January 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Yi Zheng, Radia Aboura, Sana Boujaafar, Gabrielle Lui, Déborah Hirt, Naïm Bouazza, Frantz Foissac, Jean-Marc Treluyer, Sihem Benaboud, Inès GanaAbstractTherapeutic drug monitoring (TDM) is essential in the optimization of antiretroviral (ARV) treatments. In this work, we describe a new method for the simultaneous quantification of six molecules: the three novel ARV agents dolutegravir (DTG), elvitegravir (ELV) and rilpivirine (RPV), the first integrase inhibitor raltegravir (RAL) and its ...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 19, 2020 Category: Drugs & Pharmacology Source Type: research

Development, validation and application of a new HPLC-DAD method for simultaneous quantification of apixaban, dabigatran, edoxaban and rivaroxaban in human plasma
Publication date: Available online 14 January 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Filipa Gouveia, Joana Bicker, Joana Santos, Marília Rocha, Gilberto Alves, Amílcar Falcão, Ana FortunaAbstractDirect oral anticoagulants (DOACs) have been commonly used for the treatment of venous thromboembolism and for the prevention of stroke in patients with atrial fibrillation. Despite not being initially recommended, monitoring DOACs plasma concentrations is now recognized as essential in emergency situations and in special populations. Moreover, the inter-individual variability f...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 16, 2020 Category: Drugs & Pharmacology Source Type: research

Spectrochemical identification of kanamycin resistance genes in artificial microbial communities using Clover-assay
Publication date: Available online 15 January 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Naifu Jin, Camilo L.M. Morais, Francis L. Martin, Dayi ZhangAbstractPersistent abuse and overuse of antibiotics induces a widespread bloom of antibiotic resistance genes (ARGs) and the emergence of superbugs. A method designed to rapidly quantify ARGs in real-world scenarios is urgently needed. Here, we present an orthogonal test of heavy water and kanamycin exposure, namely, a “clover-assay”, to reveal the capability of state-of-the-art Raman microspectroscopy to identify ARGs within microbial ...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 16, 2020 Category: Drugs & Pharmacology Source Type: research

Chemical profiling of Callicarpa nudiflora and its effective compounds identification by compound-target network analysis
This study provided an opportunity to deepen the understanding of the chemical composition and the potential anti-inflammatory mechanism of C. nudiflora. (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - January 16, 2020 Category: Drugs & Pharmacology Source Type: research

Characterization of bromelain indicates a molar excess of inhibitor vs. enzyme molecules, a Jacalin-like lectin and Maillard reaction products
We described two methods to separate the inhibitor fraction from the enzyme fraction, RP-HPLC and size exclusion chromatography. A pineapple derived Jacalin-like-lectin, herein called ‘Anlec’, was identified and quantified by RP-HPLC-MS in bromelain and its content was determined to be 5%, related to all proteins in bromelain. Anlec binds specifically to mannose-containing glycans and is discussed in literature to possess anti-HIV medical potential. Bromelain could therefore be a possible and economic source for the production of Anlec. An isolation strategy of Anlec from bromelain, in high purity, is shown in ...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 16, 2020 Category: Drugs & Pharmacology Source Type: research

Development of Homogeneous Plasmonic Potency Assay Using Gold Nanoparticle Immunocomplexes
In this study, we demonstrated that the AuNP-based homogeneous plasmonic immunoassay (HPI) generated comparable potency values of a therapeutic mAb to a conventional binding ELISA in relatively shorter assay time and steps. Binding HPI can be potentially implemented as a potency assay for therapeutic mAbs in quality control laboratories.Graphical abstract (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - January 12, 2020 Category: Drugs & Pharmacology Source Type: research

Simultaneous determination of fluoxetine, venlafaxine, vortioxetine and their active metabolites in human plasma by LC-MS/MS using one-step sample preparation procedure
Publication date: Available online 11 January 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Martin Kertys, Michaela Krivosova, Igor Ondrejka, Igor Hrtanek, Ingrid Tonhajzerova, Juraj MokryAbstractThe aim of antidepressant therapy is to induce remission and prevent relapses of major depressive disorder with minimum adverse effects during the treatment. Due to high variability in metabolism, therapeutic drug monitoring is recommended as a useful tool for individualisation of the therapy. For this purpose, we have developed simple and sensitive ultra-high performance liquid chromatography-tandem mass...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 12, 2020 Category: Drugs & Pharmacology Source Type: research

Spectroscopic characterisation of a series of Salmonella Typhi Vi-diphtheria toxoid glycoconjugate antigens differing in polysaccharide-protein ratio
We describe here the further spectroscopic characterisation of this series of glycoconjugate immunogen bulks using NMR spectroscopy, circular dichroism and absorption spectroscopy. We have used several mathematical approaches to extract information from the spectroscopic data not previously applied to glycoconjugates. These complementary approaches provide information on (i) the integrity of the carrier protein, (ii) consistency between batches of vaccine components, (iii) the polysaccharide: protein ratio (iv) the O-acetylation of the Vi in the conjugate (v) the stability of the O-acetylation of the Vi, and (vi) the prese...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 10, 2020 Category: Drugs & Pharmacology Source Type: research

Simultaneous determination of a promising anti-brain tumor agent CAT3 and its two major metabolites in mouse plasma and brain by a LC-MS/MS method
Publication date: Available online 9 January 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Shengyu Zhao, Ru-Bing Wang, Jie Bai, Xiaoqing Fan, Minwan Hu, Baolian Wang, Jinping Hu, Yan LiAbstractA rapid and reproducible method with high selectivity was developed for simultaneous determination of a promising anti-brain tumor agent CAT3 and its two metabolites PF403 and GLU-PF403 in mouse plasma and brain. An economic deproteinization with septuple acetonitrile (v/v) was applied to pretreat the samples in this study. All analytes were well retained and separated on a CAPCELL CORE PC (2.7 μm,...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 10, 2020 Category: Drugs & Pharmacology Source Type: research

Stability of Sodium Ascorbyl Phosphate in the Water-Glycerol System
Publication date: Available online 9 January 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Xiaohui Dong, Ting Zhang, Hongyuan Wei, Leping DangAbstractSodium ascorbyl phosphate is a hydrophilic derivative of ascorbic acid with better stability compared to the parent compound. However, sodium ascorbyl phosphate is not as stable in solution as it is in the solid state, and it has been found to degrade, with accompanying discoloration, under the influence of different conditions. Here, the degradation mechanism of sodium ascorbyl phosphate in the water-glycerol system was revealed and the thermal degr...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 10, 2020 Category: Drugs & Pharmacology Source Type: research

The Simultaneous Quantification of Phytosterols And Tocopherols in Liposomal Formulations Using Validated Atmospheric Pressure Chemical Ionization- Liquid Chromatography –Tandem Mass Spectrometry
Publication date: Available online 9 January 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Asmita Poudel, George Gachumi, Ildiko Badea, Zafer Dallal Bashi, Anas El-AneedAbstractA novel liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed and validated to simultaneously quantify phytosterols (brassicasterol, campesterol, stigmasterol and β-sitosterol) and tocopherols (alpha, beta, gamma and delta) entrapped in the lipid bilayer of a liposomal formulation. Atmospheric pressure chemical ionization (APCI) was employed due to the enhanced ionization of phytosterols and to...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 10, 2020 Category: Drugs & Pharmacology Source Type: research

Simplification of affinity macroporous monolith microfluidic column synthesis and its ability for protein separation
Publication date: Available online 8 January 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Ashish Khaparde, Kishore K.R. TetalaAbstractA generic multi-component approach was designed to perform simultaneous in situ polymerization and ligand immobilization to develop affinity porous polymer based chromatography resin in a facile mode. This strategy exploits the regioselective ring opening reaction between epoxy group of monomer and native functional group of ligand (i.e. amine) under aqueous condition (pH 9.7).As a proof-of-concept, reaction of iminodiacetic acid (IDA) with allyl glycidyl ether (AG...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 9, 2020 Category: Drugs & Pharmacology Source Type: research

On-Flow LC-MS/MS Method for Screening of Xanthine Oxidase Inhibitors
Publication date: Available online 7 January 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Marili V.N. Rodrigues, Caio Rodrigues-Silva, Sinésio Boaventura, Adriana S.S. Oliveira, Susanne Rath, Quezia B. CassAbstractThe screening of compounds is the initial step in research for the development of new drugs. For this reason, the availability of fast and reliable tools for the screening of a large number of compounds becomes essential. Among the therapeutic targets, the enzyme xanthine oxidase (XO) is of great interest for its importance as a biological source of superoxide radicals, which con...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 9, 2020 Category: Drugs & Pharmacology Source Type: research

Characterization of bromelain indicates a molar excess of inhibitor vs enzyme molecules, a Jacalin-like lectin and Maillard reaction products
We described two methods to separate the inhibitor fraction from the enzyme fraction, RP-HPLC and size exclusion chromatography. A pineapple derived Jacalin-like-lectin, herein called ‘Anlec’, was identified and quantified by RP-HPLC-MS in bromelain and its content was determined to be 5 %, related to all proteins in bromelain. Anlec binds specifically to mannose-containing glycans and is discussed in literature to possess anti-HIV medical potential. Bromelain could therefore be a possible and economic source for the production of Anlec. An isolation strategy of Anlec from bromelain, in high purity, is sho...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 9, 2020 Category: Drugs & Pharmacology Source Type: research