Development of a novel LC-MS/MS method for quantitation of triticonazole enantiomers in rat plasma and tissues and application to study on toxicokinetics and tissue distribution
In this study, a chiral analytical method of LC-MS/MS was developed and validated for the assay of triticonazole enantiomers in rat plasma and tissues. There were no endogenous interferences in the blank plasma and tissues of rats. R-(-)- and S-(+)-triticonazole peaks were separated entirely. The calibration curves were linear from 25 to 2500 ng/mL of each enantiomer. The accuracy, precision, and stability met the requirements of bioanalysis. Therefore, this method is enantioselective, accurate, precise, sensitive and reliable, and has been successfully applied to analyze R-(-)- and S-(+)-triticonazole in rat plasma...
Source: Journal of Pharmaceutical and Biomedical Analysis - April 21, 2019 Category: Drugs & Pharmacology Source Type: research

Comparative pharmacokinetic study on phenolic acids and flavonoids in spinal cord injury rats plasma by UPLC-MS/MS after single and combined oral administration of danshen and huangqin extract
Publication date: Available online 20 April 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Lixin Zhang, Xifang Liu, Hao Yang, Rui Zhao, Ciucui Liu, Rui Zhang, Qian ZhangAbstractChinese medicinal herbs danshen and huangqin have attracted attention in spinal cord injury (SCI) treatment. Purpose of this study was to investigate and compare the pharmacokinetic characteristics of 4 phenolic acids and 4 flavonoids in SCI rat plasma after orally administrate danshen, huangqin and combined extract of these two herbs (CDH). Thus, a rapid and sensitive ultra performance liquid chromatography-tandem mass spec...
Source: Journal of Pharmaceutical and Biomedical Analysis - April 21, 2019 Category: Drugs & Pharmacology Source Type: research

Development and validation of an LC-MS/MS method for the analysis of ivermectin in plasma, whole blood, and dried blood spots using a fully automatic extraction system
Publication date: 5 August 2019Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 172Author(s): Urs Duthaler, Claudia Suenderhauf, Stefan Gaugler, Beatrice Vetter, Stephan Krähenbühl, Felix HammannAbstractIvermectin is deployed in mass drug administration (MDA) campaigns to control parasitic diseases in the tropics, with billions of treatments having been administered in the last three decades. Simple blood sampling tools, like the dried blood spots (DBS) technique, are needed to monitor treatments in such challenging settings. Thus, we developed a fully automated method for the analysis of ivermec...
Source: Journal of Pharmaceutical and Biomedical Analysis - April 21, 2019 Category: Drugs & Pharmacology Source Type: research

Selective modification of skin barrier lipids
This study assess the effect of Ac on skin barrier lipids in-depth. Synchrotron based Fourier-transform infrared microspectroscopy (FTIR), which is used to study SC lipid organization, revealed a more ordered lipid organization after Ac treatment. In vitro experiments using Franz cells, which were selected to follow the SC barrier function capability, demonstrated that Ac-treated skin retained caffeine and ibuprofen on the SC with very low permeation of both compounds into the deeper skin layers. In vitro transepidermal water loss (TEWL) measurements revealed the ability of Ac to induce a less water permeated skin. Althoug...
Source: Journal of Pharmaceutical and Biomedical Analysis - April 20, 2019 Category: Drugs & Pharmacology Source Type: research

Development and validation of a UPLC-MS/MS analytical method for dofetilide in mouse plasma and urine, and its application to pharmacokinetic study
Publication date: Available online 19 April 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Muhammad Erfan Uddin, Xinxin Sun, Kevin M. Huang, Shuiying Hu, Cynthia A. Carnes, Alex Sparreboom, Qiang FuAbstractA novel method using UPLC with tandem mass-spectrometric detection (UPLC-MS/MS) with positive electrospray ionization was developed for the detection of the antiarrhythmic drug, dofetilide, in mouse plasma and urine. Protein precipitation was performed on 10 µL of plasma and 2 µL of urine samples using dofetilide-D4 as an internal standard, and separation of the analyte ...
Source: Journal of Pharmaceutical and Biomedical Analysis - April 20, 2019 Category: Drugs & Pharmacology Source Type: research

A qHNMR method for simultaneous quantification of terpenoids from Ferula ovina (Boiss.) Boiss roots
Publication date: Available online 19 April 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Faegheh Farhadi, Saba Soltani, Satar Saberi, Mehrdad IranshahiAbstractFerula ovina (Boiss.) Boiss is one of the most important endemic medicinal plants in Iran, which has three main terpenoid compounds including ferutinin, stylosin and tschimgine. Ferutinin is the strongest natural phytoestrogen that has agonistic activity on estrogen receptors, particularly α-receptors. To determine the amount of ferutinin in F. ovina roots, we firstly used HPLC-UV method. In the HPLC method, the resolution of ferutini...
Source: Journal of Pharmaceutical and Biomedical Analysis - April 20, 2019 Category: Drugs & Pharmacology Source Type: research

Development and validation of a multiplexed drug level assay in support of combination biologics therapy clinical studies
Publication date: 15 July 2019Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 171Author(s): Huaping Tang, Ketal Shah, Thomas Steinmetz, Anson Abraham, Hans Jensen, Andrew Bouton, Kasia Marullo, Shuangping ShiAbstractClinical development of biotherapeutics for combination therapy requires monitoring the concentrations of both drugs in biological samples. Traditionally, two assays are required to measure drug levels one at a time, which poses challenges in sample management, data reporting, and cost. The Meso Scale Discovery (MSD®) U-PLEX™ platform provides a simple and flexible way to create cust...
Source: Journal of Pharmaceutical and Biomedical Analysis - April 20, 2019 Category: Drugs & Pharmacology Source Type: research

A simple and sensitive LC-MS/MS method for quantification of Bepridil in rat plasma and its application to pharmacokinetic studies
Publication date: Available online 18 April 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Bhavesh D. Patel, Nageswararao P, Rajesh Alamanda, Ajit R. Gadekar, Amol A. Raje, Rakshit AmetaAbstractBepridil is potent inhibitor of Na+, K+ and Ca+ channel in cardiomyocytes. It has demonstrated strong antianginal effect with type I antiarrhythmic and with minimum antihypertensive therapeutic effect. Till date, a specific LC-MS/MS method to quantify Bepridil concentrations in biological matrix have not been reported yet.. In current study, a highly sensitive, specific and simple LC-MS/MS method for quantif...
Source: Journal of Pharmaceutical and Biomedical Analysis - April 20, 2019 Category: Drugs & Pharmacology Source Type: research

Quantification of the tyrosine kinase inhibitor erlotinib in human scalp hair by liquid chromatography-tandem mass spectrometry: pitfalls for clinical application
In conclusion, a reliable and robust LC-MS/MS method was developed to quantify erlotinib in hair, however, clinical and in vitro evaluations showed that the method is not suitable for monitoring long term erlotinib exposure. The pitfalls of this application outweigh the current benefits. (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - April 18, 2019 Category: Drugs & Pharmacology Source Type: research

Salting out assisted liquid-liquid extraction for liquid chromatography tandem-mass spectrometry determination of amphetamine-like stimulants in meconium
Publication date: Available online 17 April 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Alžběta Nemeškalová, Miroslava Bursová, David Sýkora, Martin Kuchař, Radomír Čabala, Tomáš HložekAbstractIn the last decade there has been a dramatic increase in the availability and abuse of synthetic cathinones – new amphetamine-like stimulants. Even though their abuse during pregnancy could have serious adverse effects on the fetus, cathinones are not readily included in neonatal toxicological screenings. Meconium (first neonatal stool) is the spe...
Source: Journal of Pharmaceutical and Biomedical Analysis - April 18, 2019 Category: Drugs & Pharmacology Source Type: research

Novel chiral metal organic frameworks functionalized composites for facile preparation of optically pure propranolol hydrochlorides
Publication date: Available online 16 April 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Xue Ma, Wenrui Du, Yan Li, Chenfeng Hua, Ajuan Yu, Wuduo Zhao, Shusheng Zhang, Fuwei XieABSTRACTTwo novel sorbents based on polydopamine (PDA)-coated magnetic graphite oxide-metal organic frameworks nanoparticles [Cu(L-mal)(bpy)]·H2O (MGO-CuLBH) and [Cu(D-mal)(bpy)]·H2O (MGO-CuDBH) possessing of both magnetic property and excellent enantioselective ability were prepared and characterized. Solutions of racemic propranolol hydrochloride (Rac-PRO) were chosen to investigate the enantioselective per...
Source: Journal of Pharmaceutical and Biomedical Analysis - April 17, 2019 Category: Drugs & Pharmacology Source Type: research

Pharmacokinetic Properties of Enantiomerically Pure GluN2B Selective NMDA Receptor Antagonists with 3-Benzazepine Scaffold
Publication date: Available online 16 April 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Frederik Börgel, Fabian Galla, Kirstin Lehmkuhl, Dirk Schepmann, Simon M. Ametamey, Bernhard WünschAbstractRecently, the eutomers of highly potent GluN2B selective NMDA receptor antagonists with 3-benzazepine scaffold were identified. Herein, pharmacokinetic properties regarding lipophilicity, plasma protein binding (PPB) and metabolism are analyzed. The logD7.4 values of 1.68 for phenol 1 and 2.46 for methyl ether 2 are in a very good range for CNS agents. A very similar logD7.4 value was recorded ...
Source: Journal of Pharmaceutical and Biomedical Analysis - April 17, 2019 Category: Drugs & Pharmacology Source Type: research

The contamination of valsartan and other sartans, Part 2: Untargeted Screening reveals contamination with amides additionally to known nitrosamine impurities
Publication date: Available online 16 April 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Oliver Scherf-Clavel, Martina Kinzig, Axel Besa, André Schreiber, Clemens Bidmon, Mona Abdel-Tawab, Jonas Wohlfart, Fritz Sörgel, Ulrike HolzgrabeAbstractThe recent incidences of contaminated valsartan drug products gave rise to review the suitability of current impurity profiling workflows implemented at authorities and pharmaceutical companies. The major drawback of targeted impurity profiling, where a considerable amount of prior knowledge about possible contaminants is necessary, is the fact t...
Source: Journal of Pharmaceutical and Biomedical Analysis - April 17, 2019 Category: Drugs & Pharmacology Source Type: research

Simultaneous determination of curcumin, tetrahydrocurcumin, quercetin, and paeoniflorin by UHPLC-MS/MS in rat plasma and its application to a pharmacokinetic study
In this study, a rapid, sensitive, and reliable ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method was developed and validated for the simultaneous determination of CUR, tetrahydrocurcumin (THC), QR, and PF in rat plasma by using tinidazole as the internal standard (IS). A liquid-liquid extraction method with ethyl acetate was used to pre-treat the plasma samples. Chromatographic separation was conducted on a C18 column with isocratic elution using acetonitrile and 0.1% formic acid water solution (80:20, v/v) as the mobile phase at the flow rate of 0.3 mL/min. A TSQ Quantum Ac...
Source: Journal of Pharmaceutical and Biomedical Analysis - April 17, 2019 Category: Drugs & Pharmacology Source Type: research

The application of combined 1H NMR-based metabolomics and transcriptomics techniques to explore phenolic acid biosynthesis in Salvia miltiorrhiza Bunge
Publication date: Available online 17 April 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Xia Liu, Mengxia Jin, Min Zhang, Tianqi Li, Shanshan Sun, Jinyue Zhang, Jungui Dai, Yinghong WangAbstractSalvia miltiorrhiza Bunge is a traditional Chinese medicine, and its water-soluble phenolic acid active compounds have very important medicinal value; however, the synthesis pathways of the main active ingredients remain unknown. Here, we employed nuclear magnetic resonance (NMR)-based metabolomics and transcriptomics techniques to study the biosynthesis mechanism of salvianolic acids. High-performance liq...
Source: Journal of Pharmaceutical and Biomedical Analysis - April 17, 2019 Category: Drugs & Pharmacology Source Type: research

Analysis of hydroxy-cocaine metabolites as evidence of cocaine consumption: identification by parent ion search and quantitation by uhplc–ms/ms in hair sample
Publication date: Available online 15 April 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Mauro Minoli, Sara Casati, Ilaria Angeli, Alessandro Ravelli, Paola Rota, Pietro Allevi, Marica OrioliAbstractOver the last decade, hair analysis has become a routine procedure in most forensic laboratories and, complementary to blood and urine, hair is a unique biological matrix which gives the opportunity to establish a temporal consumption profile. Despite hair is widely used to identify drug use, environmental contamination continues to represent a challenging factor of this procedure, especially for coca...
Source: Journal of Pharmaceutical and Biomedical Analysis - April 17, 2019 Category: Drugs & Pharmacology Source Type: research

Multi-class determination of steroid hormones and antibiotics in fatty hotpot ingredients by pressurized liquid extraction and liquid chromatography–tandem mass spectrometry
Publication date: Available online 15 April 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Mengting Wang, Yang Wang, Bin Peng, Na Zhao, Jing Zhao, Zhili Xiong, Longshan ZhaoAbstractDue to the misuse of hormones and antibiotics resulting in drug residues in meat products, serious concerns have arisen regarding to the potential risks for human health. In this paper, a simple and efficient method was developed to simultaneously quantify six steroid hormones and six antibiotics in fatty foods by combining pressurized liquid extraction (PLE) with ultra-high performance liquid chromatography-tandem mass ...
Source: Journal of Pharmaceutical and Biomedical Analysis - April 17, 2019 Category: Drugs & Pharmacology Source Type: research

Quantitation of paclitaxel, and its 6-alpha-OH and 3-para-OH metabolites in human plasma by LC-MS/MS
Publication date: Available online 15 April 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Susan M. Christner, Robert A. Parise, Percy S. Ivy, Hussein Tawbi, Edward Chu, Jan H. BeumerAbstractWe have developed a high performance liquid chromatography mass spectrometry method for quantitating paclitaxel and its 6-alpha-OH and 3-para-OH metabolites in 0.1 mL human plasma. After MTBE liquid-liquid extraction, chromatographic separation was achieved with a Phenomenex synergy polar reverse phase (4 µm, 2 mm x 50 mm) column and a gradient of 0.1% formic acid...
Source: Journal of Pharmaceutical and Biomedical Analysis - April 17, 2019 Category: Drugs & Pharmacology Source Type: research

Development and validation of a LC-MS/MS method for the analysis of ivermectin in plasma, whole blood, and dried blood spots using a fully automatic extraction system
Publication date: Available online 16 April 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Urs Duthaler, Claudia Suenderhauf, Stefan Gaugler, Beatrice Vetter, Stephan Krähenbühl, Felix HammannAbstractIvermectin is deployed in mass drug administration (MDA) campaigns to control parasitic diseases in the tropics, with billions of treatments having been administered in the last three decades. Simple blood sampling tools, like the dried blood spots (DBS) technique, are needed to monitor treatments in such challenging settings. Thus, we developed a fully automated method for the analysis of iv...
Source: Journal of Pharmaceutical and Biomedical Analysis - April 17, 2019 Category: Drugs & Pharmacology Source Type: research

Belizatinib: Novel reactive intermediates and bioactivation pathways characterized by LC–MS/MS
Publication date: 15 July 2019Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 171Author(s): Mohamed W. Attwa, Adnan A. Kadi, Hany W. DarwishAbstractBelizatinib (BZB; TSR-011) is a next-generation anaplastic lymphoma kinase inhibitor that also inhibits tropomyosin-related kinases A/B/C. In this in-vitro study, we examined the formation of reactive metabolites from BZB using rat liver microsomes or human liver microsomes in the presence of a trapping agent (potassium cyanide) to generate iminium reactive intermediates. Identification of the in vitro BZB metabolites indicated that the major in-vitro metaboli...
Source: Journal of Pharmaceutical and Biomedical Analysis - April 17, 2019 Category: Drugs & Pharmacology Source Type: research

Study of the in vitro metabolic profile of a new α2-adrenergic agonist in rat and human liver microsomes by using liquid chromatography-multiple-stage mass spectrometry and nuclear magnetic resonance
Publication date: Available online 14 April 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Josiane de O. Cardoso, Bianca F. da Silva, Tiago Venâncio, Marina G. da Rocha Pitta, Ivan da R. Pitta, Rosângela G. Peccinini, Regina V. OliveiraAbstractA potent synthetic α2-adrenergic agonist called PT-31, (3-(2-chloro-6-fluorobenzyl)-imidazolidine-2,4-dione), was recently detected as a potential drug to be used as an adjuvant drug to treat chronic pain. The excellent pharmacological property of PT-31 highlights the importance in elucidating its metabolism, which could provide valuable inf...
Source: Journal of Pharmaceutical and Biomedical Analysis - April 16, 2019 Category: Drugs & Pharmacology Source Type: research

An optimized analytical method for cellular targeted quantification of primary metabolites in tricarboxylic acid cycle and glycolysis using gas chromatography-tandem mass spectrometry and its application in three kinds of hepatic cell lines
In this study, we established a repeatable gas chromatography-tandem mass spectrometry (GC-MS/MS) method with selected reaction monitoring (SRM) mode for simultaneous quantification of several primary metabolites in these two pathways, including glucose, 3-phosphoglycerate, phosphoenolpyruvate (PEP), pyruvate, lactate, citrate, cis-aconitate, isocitrate, α-ketoglutarate, succinate, fumarate and malate. There are many solvents to extract the metabolites in these two pathways, however, which one is more effective still remains unclear. Sample pretreatment was optimized for solvent types and volumes to advance the extra...
Source: Journal of Pharmaceutical and Biomedical Analysis - April 14, 2019 Category: Drugs & Pharmacology Source Type: research

Degradation studies of ibrutinib under stress conditions: characterisation and structural elucidation of novel degradants
Publication date: Available online 12 April 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Soujanya Vajjha, Vijay Bommuluri, KVK Mohan P, Chidananda Swamy Rumalla, Raju Doddipalla, Muralidharan Kaliyaperumal, Raghu Babu KorupoluAbstractThe aim of the research work is to study the degradation behaviour of Ibrutinib (IBN) which is performed under different stress conditions according to International Conference on Harmonization guidelines (ICH). The study included monitoring degradation of the Ibrutinib drug under acidic, base, oxidation, thermal and photolytic conditions followed by isolation and ch...
Source: Journal of Pharmaceutical and Biomedical Analysis - April 14, 2019 Category: Drugs & Pharmacology Source Type: research

Development and validation of a highly sensitive LC-MS/MS method for in vitro measurement of histamine concentration
In this study, a bioanalytical LC-MS/MS method was developed and validated to quantify histamine in the leukocyte suspension from human peripheral blood. The method used pre-column derivatization with phenylisothiocyanate (PITC) and the resulting phenylthiocarbamyl (PTC) histamine was analyzed by positive-ion electrospray ionization using the multiple reaction monitoring mode. Chromatographic separation was achieved using an Imtakt-HT C18 column (2.1 mm × 50 mm, 3.0 μm), with a flow rate of 0.35 mL/min, 2 μL injection, and gradient elution with a mixture of aceto...
Source: Journal of Pharmaceutical and Biomedical Analysis - April 14, 2019 Category: Drugs & Pharmacology Source Type: research

DeFvelopment and Validation of a Multiplexed Drug Level Assay in Support of Combination Biologics Therapy Clinical Studies
Publication date: Available online 13 April 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Huaping Tang, Ketal Shah, Thomas Steinmetz, Anson Abraham, Hans Jensen, Andrew Bouton, Kasia Marullo, Shuangping ShiAbstractClinical development of biotherapeutics for combination therapy requires monitoring the concentrations of both drugs in biological samples. Traditionally, two assays are required to measure drug levels one at a time, which poses challenges in sample management, data reporting, and cost. The Meso Scale Discovery (MSD®) U-PLEXTM platform provides a simple and flexible way to create cus...
Source: Journal of Pharmaceutical and Biomedical Analysis - April 14, 2019 Category: Drugs & Pharmacology Source Type: research

Measurement of ultra-trace level of intact oxytocin in plasma using SALLE combined with nano-LC-MS
In this study, we developed an efficient salt-out assisted liquid-liquid extraction (SALLE) to treat plasma, and then analyzed the samples using nano-LC-MS to quantify intact oxytocin (OT) in human and rat plasmas. Our results showed that the use of SALLE (Isopropanol/K2HPO4 (4 M)) allows efficient removal of various disrupters, including proteins, inorganic salts, and lipids, which helps avoid the risk of blocked capillary columns and matrix effects. Moreover, instant SALLE can reduce the possible binding between OT and proteins, thus allowing high repeatability of OT extraction from the original plasma. This combi...
Source: Journal of Pharmaceutical and Biomedical Analysis - April 14, 2019 Category: Drugs & Pharmacology Source Type: research

A new strategy for extraction and depuration of pantoprazole in rat plasma: vortex assisted dispersive micro-solid-phase extraction employing metal organic framework MIL-101(Cr) as sorbent followed by dispersive liquid-liquid microextraction based on solidification of a floating organic droplet
Publication date: Available online 11 April 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Qianqian Cai, Tianyu Zhao, Lianjun Zhang, Pan Zhao, Yajie Zhu, Haiyan Xu, Xiaohong HouAbstractDispersive micro-solid-phase extraction (DMSPE) combined with dispersive liquid-liquid microextraction based on the solidification of a floating organic droplet (DLLME-SFO) was successfully developed for extraction and depuration of pantoprazole in rat plasma. The remarkable metal organic framework (MOF), MIL-101(Cr) was used as DMSPE adsorbent. The detection of pantoprazole was performed by convenient HPLC-UV. In th...
Source: Journal of Pharmaceutical and Biomedical Analysis - April 12, 2019 Category: Drugs & Pharmacology Source Type: research

Reply to “Requirements for accurate 1H NMR quantification of mineral oil hydrocarbons (paraffins) for pharmaceutical or cosmetic use”
Publication date: Available online 10 April 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Jonas Urlaub, Jochen Norwig, Curd Schollmayer, Ulrike Holzgrabe (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - April 11, 2019 Category: Drugs & Pharmacology Source Type: research

Comparison of isotope pattern deconvolution and calibration curve quantification methods for the determination of estrone and 17β-estradiol in human serum
Publication date: Available online 9 April 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): J. Pitarch-Motellón, N. Fabregat-Cabello, C. Le Goff, A.F. Roig-Navarro, J.V. Sancho-Llopis, E. CavalierAbstractA Liquid Chromatography coupled to tandem mass spectrometry (LC-MS/MS) based method have been developed for the determination of the main estrogen compounds –estrone (E1) and 17β-estradiol (E2)– in human serum. Two isotope dilution mass spectrometry (IDMS) quantification procedures have been used: a classical calibration curve-based method were compared to a recently developed...
Source: Journal of Pharmaceutical and Biomedical Analysis - April 10, 2019 Category: Drugs & Pharmacology Source Type: research

Pharmaceutical and preclinical evaluation of Advax adjuvant as a dose-sparing strategy for ant venom immunotherapy
Publication date: Available online 9 April 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Troy Wanandy, Yoshikazu Honda-Okubo, Noel W. Davies, Hayley E. Rose, Robert J. Heddle, Simon G.A. Brown, Richard Woodman, Nikolai Petrovsky, Michael D. WieseAbstractA major challenge in broader clinical application of Jack Jumper ant venom immunotherapy (JJA VIT) is the scarcity of ant venom which needs to be manually harvested from wild ants. Adjuvants are commonly used for antigen sparing in other vaccines, and thereby could potentially have major benefits to extend JJA supplies if they were to similarly enh...
Source: Journal of Pharmaceutical and Biomedical Analysis - April 10, 2019 Category: Drugs & Pharmacology Source Type: research

Rapid quantitative analysis of ginkgo flavonoids using paper spray mass spectrometry
Publication date: Available online 10 April 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Wei Zhou, Zihui Yang, Si Huang, Zhengfa Fang, Bo Chen, Ming MaAbstractIn this work, a method for the rapid and reliable analysis of ginkgo flavonoids was developed by using paper spray mass spectrometry (PS-MS) assisted by microwave-assisted hydrolysis (MAH). Kaempferol-D4, quercetin-D5, and isorhamnetin-D3 were used as internal standards (IS). Samples analyses were completed by simply treating with extraction, MAH, dilution, and quantitative analysis by PS-MS. Because of the absence of HPLC separation, the P...
Source: Journal of Pharmaceutical and Biomedical Analysis - April 10, 2019 Category: Drugs & Pharmacology Source Type: research

A systemic combined nontargeted and targeted LC-MS based metabolomic strategy of plasma and liver on pathology exploration of alpha-naphthylisothiocyanate induced cholestatic liver injury in mice
In conclusion, CLI might correlate significantly with hepatocyte necrosis, metabolic disorders and imbalance of intestinal microbiome ecology triggered by BA accumulation.Graphical abstract (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - April 9, 2019 Category: Drugs & Pharmacology Source Type: research

Speciation analysis of arsenic in urine samples from APL patients treated with single agent As2O3 by HPLC-HG-AFS
Publication date: Available online 8 April 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Meihua Guo, Jing Li, Shengjin Fan, Wensheng Liu, Bin Wang, Chunlu Gao, Jin Zhou, Xin HaiAbstractArsenic trioxide [As2O3, arsenite (AsIII) in solution] has been applied successfully for the treatment of acute promyelocytic leukemia (APL). The arsenic speciation analysis of urine is critical to reveal the metabolic mechanism and the relationship between arsenic species and the clinical response. To characterize the arsenic species in urine, a simple and robust HPLC-HG-AFS method was developed and validated to qu...
Source: Journal of Pharmaceutical and Biomedical Analysis - April 9, 2019 Category: Drugs & Pharmacology Source Type: research

Chemical fingerprints, antioxidant, enzyme inhibitory, and cell assays of three extracts obtained from Sideritis ozturkii Aytaç & Aksoy: An endemic plant from Turkey
This study was geared towards assessing the possible antioxidant, enzyme inhibitory, and cytotoxic activities of ethyl acetate, methanol, and water extracts of Sideritis ozturkii Aytaç & Aksoy. The phytochemical profiles of the studied extracts were characterised by HPLC-MS/MS. The methanol extract, rich in phenolics (78.04 mg gallic acid equivalent/g), exhibited the strongest antioxidant activities. However, the ethyl acetate extract was the most active extract in the enzyme inhibitory assays. The water extract of S. ozturkii (1 mg/ml, 48 h incubation) slightly inhibited (22%) growth of hum...
Source: Journal of Pharmaceutical and Biomedical Analysis - April 9, 2019 Category: Drugs & Pharmacology Source Type: research

A new strategy based on microwave-assisted technology for the extraction and purification of beeswax policosanols for pharmaceutical purposes and beyond
Publication date: Available online 9 April 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Alberto Venturelli, Virginia Brighenti, Danilo Mascolo, Maria Paola Costi, Federica PellatiAbstractPolicosanols (PCs) are a mixture of long chain primary aliphatic alcohols mainly known for their ability to reduce cholesterol level. Due to this property, there is an increasing interest in the extraction process of these compounds. In this context, beeswax, a natural product produced by honey bees of the genus Apis, is a promising source for their extraction and purification. The present research work was aimed...
Source: Journal of Pharmaceutical and Biomedical Analysis - April 9, 2019 Category: Drugs & Pharmacology Source Type: research

Determination of fascaplysin in rat plasma with ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS): Application to a pharmacokinetic study
Publication date: Available online 8 April 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Zhiyan Hu, Manman Zhang, Ming He, Bingqing Fang, Xiujie Bao, Xiaojun Yan, Hongze Liang, Haixing WangAbstractA rapid and sensitive ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed to determine fascaplysin in rat plasma using methylfascaplysin as an internal standard. Both fascaplysin and methylfascaplysin were synthesized in our laboratory. Protein precipitation was applied for the preparation of the plasma sample. Chromatographic separation was performed on a r...
Source: Journal of Pharmaceutical and Biomedical Analysis - April 9, 2019 Category: Drugs & Pharmacology Source Type: research

Belizatinib: Novel Reactive Intermediates and Bioactivation Pathways Characterized by LC-MS/MS
Publication date: Available online 6 April 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Mohamed W. Attwa, Adnan A. Kadi, Hany W. DarwishAbstractBelizatinib (BZB; TSR-011) is a next-generation anaplastic lymphoma kinase inhibitor that also inhibits tropomyosin-related kinases A/B/C. In this in-vitro study, we examined the formation of reactive metabolites from BZB using rat liver microsomes or human liver microsomes in the presence of a trapping agent (potassium cyanide) to generate iminium reactive intermediates. Identification of the in vitro BZB metabolites indicated that the major in-vitro met...
Source: Journal of Pharmaceutical and Biomedical Analysis - April 7, 2019 Category: Drugs & Pharmacology Source Type: research

Novel Urchin-like FeCo Oxide Nanostructures Supported Carbon Spheres as a Highly Sensitive Sensor for Hydrazine Sensing Application
Publication date: Available online 6 April 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): D.M. Nguyen, L.G. Bach, Q.B. BuiAbstractHerein, we successfully fabricated a novel nanostructure based on hierarchical urchin-like FeCo oxide supported carbon spheres (FeCo Oxide/CSs) via a two-step hydrothermal method followed by a simple annealing step at 300 °C under air. It was found that such urchin-like FeCo Oxide/CSs structure exhibited superior catalytic activity towards hydrazine oxidation to CSs, Fe Oxide/CSs, Co Oxide/CSs, and FeCo Hydroxide/CSs material. In this regard, the FeCo Oxide/CS...
Source: Journal of Pharmaceutical and Biomedical Analysis - April 7, 2019 Category: Drugs & Pharmacology Source Type: research

A clinical study for the evaluation of pharmacokinetic interaction between daclatasvir and fluoxetine
Publication date: Available online 5 April 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Mohamed Oraby, Ahmed Khorshed, Eman Abdul-Rahman, Ramadan Ali, Mohamed M ElsutohyAbstractA simple and sensitive chromatographic method has been developed for the quantitative analysis of an antiviral agent, daclatasvir (DCV), that commonly prescribed for the treatment of hepatitis C viral (HCV) infection. The method was applied to detect DCV in human plasma and real blood samples collected from patients diagnosed with HCV and treated with DCV. The analysis strategy was based on recording the native fluorescenc...
Source: Journal of Pharmaceutical and Biomedical Analysis - April 6, 2019 Category: Drugs & Pharmacology Source Type: research

Pharmacokinetics of 40 kDa PEG in rodents using high-field NMR spectroscopy
Publication date: 15 July 2019Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 171Author(s): Purnima Khandelwal, Lisa Zhang, Anjaneya Chimalakonda, Janet Caceres-Cortes, Christine Huang, Punit Marathe, Michael D. ReilyAbstractConjugation of macromolecular drugs to polyethylene glycol (PEG) improves their therapeutic potential by reducing their rate of degradation, thereby extending the drugs half life. As a substantial component of the drug, it is necessary to measure the pharmacokinetic (PK) characteristics of PEG in vivo. A quantitative NMR-based method was developed and successfully applied to measuring...
Source: Journal of Pharmaceutical and Biomedical Analysis - April 6, 2019 Category: Drugs & Pharmacology Source Type: research

Measurement uncertainty and risk of false conformity decision in the performance evaluation of liquid chromatography analytical procedures
Publication date: Available online 4 April 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Luciana Separovic, Felipe Rebello LourençoAbstractLiquid chromatography is one of the main techniques used in pharmaceutical quality control analytical procedures. However, there will always be a measurement uncertainty (MU) associated with them, that can lead to the approval of an out of specification lot (consumer risk) or rejection of a lot within specification (producer risk). Thus, the aim of this study was to evaluate the performance of liquid chromatography analytical procedures based on their me...
Source: Journal of Pharmaceutical and Biomedical Analysis - April 6, 2019 Category: Drugs & Pharmacology Source Type: research

Lysophospholipid profiles of apolipoprotein E-deficient mice reveal potential lipid biomarkers associated with atherosclerosis progression using validated UPLC-QTRAP-MS/MS-based lipidomics approach
In conclusion, these results provided detailed changes of Lyso-PL profiles associated with atherosclerosis and the differential Lyso-PLs with reasonable change trends may serve as promising biomarkers for atherosclerosis progression.Graphical abstract (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - April 6, 2019 Category: Drugs & Pharmacology Source Type: research

Pharmacokinetic comparisons of major bioactive components after oral administration of raw and steamed rhubarb by UPLC-MS/MS
Publication date: Available online 2 April 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Jing Zhang, Yadong Fu, Li Li, Ying Liu, Cun Zhang, Dingrong Yu, Yinlian Ma, Yongqing XiaoAbstractA study was performed to compare the pharmacokinetics of major bioactive analytes in rhubarb plants to explore the pharmacological differences between raw pieces (RP) and steamed pieces (SP) of rhubarb. A rapid and efficient ultra-performance liquid chromatographic coupled with mass/mass spectrometry (UPLC-MS/MS) method was established to determine the concentrations of six analytes in rat plasma after oral adminis...
Source: Journal of Pharmaceutical and Biomedical Analysis - April 3, 2019 Category: Drugs & Pharmacology Source Type: research

In vivo metabolic profiles of Bu-Zhong-Yi-Qi-Tang, a famous traditional Chinese medicine prescription, in rats by ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry
Publication date: Available online 3 April 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Liufang Hu, Zhihong Yao, Zifei Qin, Liyin Liu, Xiaojun Song, Yi Dai, Hiroaki Kiyohara, Haruki Yamada, Xinsheng YaoAbstractBu-Zhong-Yi-Qi-Tang (BZYQT), a famous traditional Chinese medicine prescription (TCMP), has been extensively used for conditioning sub-health status and diseases caused by spleen-qi deficiency in China for over 700 years. BZYQT is prevalent not only in China, but also in Japan and South Korea for the clinical treatment of chronic diseases, such as fatigue, tuberculosis and loss of appetite ...
Source: Journal of Pharmaceutical and Biomedical Analysis - April 3, 2019 Category: Drugs & Pharmacology Source Type: research

Structural characterization and Screening of Chemical Markers of Flavonoids in Lysimachiae Herba and Desmodii Styracifolii Herba by ultra high-performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry based metabolomics approach
Publication date: Available online 3 April 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Xiaochen Liu, Xueyan Fan, Xin Wang, Ruina Liu, Caifeng Meng, Chunying WangAbstractIn traditional Chinese medicine, Lysimachiae Herba (LH) and Desmodii Styracifolii Herba (DSH) have been widely used for the treatment of calculi, but there is a certain focus in clinical application. Flavonoids as their pharmacologically active substances were focusly studied to make clear of their chemical compositions and reveal the similarities and differences between LH and DHS by analysis of characteristic marker components ...
Source: Journal of Pharmaceutical and Biomedical Analysis - April 3, 2019 Category: Drugs & Pharmacology Source Type: research

Editorial Board
Publication date: 30 May 2019Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 169Author(s): (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - April 3, 2019 Category: Drugs & Pharmacology Source Type: research

Simultaneous analysis of volatile and semi-volatile components in a topical formulation by gas chromatography using a programmed temperature vaporization inlet and flame ionization detection
Publication date: Available online 1 April 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Felix Anyakudo, Erwin Adams, Ann Van SchepdaelAbstractTopical formulations are medications applied locally on the skin to treat ailment. They are made up of complex mixtures of active ingredients and excipients. Till date, no analytical method has been found in literature that is able to simultaneously analyze volatile and semi-volatile actives present in topical formulations. In this work, an analytical procedure by gas chromatography equipped with a programmed temperature vaporizing (PTV) inlet and a flame i...
Source: Journal of Pharmaceutical and Biomedical Analysis - April 3, 2019 Category: Drugs & Pharmacology Source Type: research

Fatty acids-based microemulsion liquid chromatographic determination of multiple caffeoylquinic acid isomers and caffeic acid in honeysuckle sample
Publication date: Available online 1 April 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Li-Jing Du, Li-Hong Ye, Jun CaoABSTRACTA green and efficient microemulsion liquid chromatographic (MELC) method using fatty acid as co-surfactant and electrochemical detection was established and validated for the determination of four caffeoylquinic acid isomers and caffeic acid in honeysuckle samples. The influences of each individual component within the isocratic oil-in-water (O/W) microemulsion mobile phase were systematically investigated, such as the type and concentration of co-surfactant, concentratio...
Source: Journal of Pharmaceutical and Biomedical Analysis - April 3, 2019 Category: Drugs & Pharmacology Source Type: research

Pharmacokinetics of 40kDa PEG in rodents using high-field NMR spectroscopy
Publication date: Available online 1 April 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Purnima Khandelwal, Lisa Zhang, Anjaneya Chimalakonda, Janet Caceres-Cortes, Christine Huang, Punit Marathe, Michael D. ReilyAbstractConjugation of macromolecular drugs to polyethylene glycol (PEG) improves their therapeutic potential by reducing their rate of degradation, thereby extending the drugs half-life. As a substantial component of the drug, it is necessary to measure the pharmacokinetic (PK) characteristics of PEG in vivo. A quantitative NMR-based method was developed and successfully applied to meas...
Source: Journal of Pharmaceutical and Biomedical Analysis - April 3, 2019 Category: Drugs & Pharmacology Source Type: research

Requirements for accurate 1H NMR quantification of mineral oil hydrocarbons (paraffins) for pharmaceutical or cosmetic use
Publication date: Available online 1 April 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Jürgen Geisser, Jan Teipel, Thomas Kuballa, Sandra Weber, Gerd Mildau, Stephan G. Walch, Dirk W. LachenmeierGraphical abstract (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - April 3, 2019 Category: Drugs & Pharmacology Source Type: research