UPLC-Q-TOF/MS-based metabolic profiling comparison of four major bioactive components in normal and CKD rat plasma, urine and feces following oral administration of Cornus officinalis Sieb and Rehmannia glutinosa Libosch herb couple extract
In this study, UPLC-Q-TOF/MS technique combined with MetabolynxTM software, was developed and successfully applied for analysis of metabolic profiles of the bioactive components of the herb couple in normal and CKD rat biological samples. Main parent components of the herb couple extract such as loganin, morroniside and catalpol were absorbed into the blood circulation of the normal and CKD rats. Another parent component acteoside was almost completely degraded. Seventeen metabolites involved in the in vivo metabolism processes were tentatively identified. These metabolites indicated that loganin was mainly metabolized to ...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 26, 2018 Category: Drugs & Pharmacology Source Type: research

Pellet Patented Technology for Fast and Distinct Visual Detection of Cholinesterase Inhibitors in Liquids
Publication date: Available online 25 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Jakub Vysloužil, David Vetchý, Jiří Zeman, Oldřich Farsa, Aleš Franc, Jan Gajdziok, Jan Vysloužil, Katarína Ficeriová, Pavel Kulich, Zbyněk Kobliha, Vladimír PitschmannAbstractThe main objective of the presented research was to prepare an innovative carrier as a filler for detection tubes in the form of double-coated pellets with a very significant color transition during the detection of cholinesterase inhibitors such as nerve agents, organophosphorus or carbam...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 26, 2018 Category: Drugs & Pharmacology Source Type: research

Green and efficient extraction of four bioactive flavonoids from Pollen Typhae by ultrasound-assisted deep eutectic solvents extraction
In this study, a highly and efficient extraction technique for determination of four bioactive flavonoids from Pollen Typhae using a combination of ultrasound-assisted extraction and natural deep eutectic solvents (NADESs) was developed. A series of DESs containing various hydrogen bond acceptors combined with different hydrogen bond donors were synthesized and screened for high extraction efficiencies based on the flavonoids extraction yields. The extraction conditions including composition of DES, water content in DES, liquid-solid ratio, and extraction time were statistically optimized by single-factor experiment. As a ...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 26, 2018 Category: Drugs & Pharmacology Source Type: research

Metabolomics in chronic kidney disease: Strategies for extended metabolome coverage
Publication date: Available online 25 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Gagnebin Yoric, Boccard Julien, Ponte Belén, Rudaz SergeABSTRACTChronic kidney disease (CKD) is becoming a major public health issue as prevalence is increasing worldwide. It also represents a major challenge for the identification of new early biomarkers, understanding of biochemical mechanisms, patient monitoring and prognosis. Each metabolite contained in a biofluid or tissue may play a role as a signal or as a driver in the development or progression of the pathology. Therefore, metabolomics is a ...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 26, 2018 Category: Drugs & Pharmacology Source Type: research

Ultrahigh-performance liquid chromatography-tandem mass spectrometry (UHPLC–MS/MS) assay for simultaneous quantifications of CZ48, lactone-stabilized camptothecin, and camptothecin and their pharmacokinetic and biliary evaluations in rats
In this study, a UHPLC–MS/MS method was developed and validated to simultaneously quantify CZ48 and CPT in rat plasma and bile. Detection was performed using the API 3200 Q Trap triple quadrupole mass spectrometer in a positive ion mode. Chromatographic separation was achieved on Waters ACQUITY UPLC BEH Shield RP18 column with a gradient elution at a flow rate of 0.45 ml/min, using mobile phases of 0.1% acetic acid in water (A) and 0.1% acetic acid in acetonitrile (B). The method was linear at the concentration ranges of 0.98 (LLOQ) −1000 ng/ml of CZ48 and CPT in rat plasma and 3.9 (LLOQ) −1...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 25, 2018 Category: Drugs & Pharmacology Source Type: research

Developing a rapid semi-automated sample preparation with alkaline hydrolysis in a 96-well plate for quantification of 11-nor-Δ9-tetrahydrocannabinol-9-carboxylic acid in urine samples by UHPLC-MS/MS
Publication date: Available online 23 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Lill Dannevig Müller, Mimi Stokke OpdalAbstractA simple, sensitive, rapid and robust manual (in glass tubes) and semi-automatic method (in a 96-well plate format) for quantification of 11-nor-Δ9-tetrahydrocannabinol-9-carboxylic acid (THCA) in urine using a UHPLC-MS/MS have been developed. The methods involved hydrolysis with potassium hydroxide in a 96-well plate, followed by neutralization and dilution of the sample in one step with a stable solution of formic acid-methanol-acetonitrile-water (2...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 24, 2018 Category: Drugs & Pharmacology Source Type: research

Modified glassy carbon electrode with Polydopamine-multiwalled carbon nanotubes for simultaneous electrochemical determination of biocompounds in biological fluids
In this study, a simple modified glassy carbon electrode with Polydopamine-multiwalled carbon nanotubes (GCE/PDA-MWCNTs) is described for selective and sensitive simultaneous voltammetric determination of dopamine (DA), acetaminophen (AC), and xanthine (XN). A detailed investigation by field emission scanning electron microscopy, Fourier transform infrared spectroscopy and electrochemistry methods such as cyclic voltammetry (CV), electrochemical impedance spectroscopy, differential pulse voltammetry (DPV) and chronoamperometry are performed in order to elucidate the preparation process and properties of the GCE/ PDA-MWCNTs...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 24, 2018 Category: Drugs & Pharmacology Source Type: research

Quantitative structure-activity relationship for estrogenic flavonoids from Psoralea corylifolia
In conclusion, molecular docking can potentially be applied for predicting the receptor-binding properties of undescribed compounds based on their molecular structure.Graphical abstract (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - August 23, 2018 Category: Drugs & Pharmacology Source Type: research

Sensitive targeted methods for brain metabolomic studies in microdialysis samples
Publication date: Available online 23 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Jana Bongaerts, Dimitri De Bundel, Debby Mangelings, Ilse Smolders, Yvan Vander Heyden, Ann Van EeckhautAbstractIn vivo determination of brain mediators plays an important role in providing insight in how the brain functions. For this purpose, targeted metabolomics can be a very useful tool. Targeted metabolomics detects and measures certain known low-molecular-weight biomolecules involved in signaling pathways and biochemical processes in the central nervous system. Microdialysis is a powerful technique to ...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 23, 2018 Category: Drugs & Pharmacology Source Type: research

First inter-laboratory study of a Supercritical Fluid Chromatography method for the determination of pharmaceutical impurities
This study involved 19 participating laboratories from 4 continents and 9 different countries. It included 5 academic groups, 3 demonstration laboratories at analytical instrument companies, 10 pharmaceutical companies and 1 food company. In the initial analysis of the study results, consistencies within- and between-laboratories were deeply examined. In the subsequent analysis, the method reproducibility was estimated taking into account variances in replicates, between-days and between-laboratories. The results obtained were compared with the literature values for liquid chromatography (LC) in the context of impurities d...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 23, 2018 Category: Drugs & Pharmacology Source Type: research

Behavior of pesticides and their metabolites in traditional Chinese medicine Paeoniae Radix Alba during processing and associated health risk
This study main investigated the dissipation and metabolism of pesticides in Chinese medicine Paeoniae Radix Alba during processing, and the calculated pesticide PFs were also used to assess the risk of dietary exposure. For analyze samples, a simple and high-throughput multiresidue pesticide analysis method was developed and validated for pesticides and their metabolites in P. Radix. based on QuEChERS procedure combined with HPLC-MS/MS. Recoveries at three concentration levels were within 61.37%–117.82% with an associated precision RSD 
Source: Journal of Pharmaceutical and Biomedical Analysis - August 21, 2018 Category: Drugs & Pharmacology Source Type: research

Liquid chromatographic techniques in betacyanin isomers separation from Gomphrena globosa L. flowers for the determination of their antimicrobial activities
Publication date: Available online 15 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Aneta Spórna-Kucab, Katarzyna Bernaś, Agnieszka Grzegorczyk, Anna Malm, Krystyna Skalicka-Woźniak, Sławomir WybraniecAbstractHigh-speed counter-current chromatography (HSCCC) combined with HPLC were established to purify, for the first time, betacyanin isomers from Gomphrena globosa L. flowers. The mixture of tert-butyl methyl ether – butanol – methanol - water TBME-BuOH-MeOH-H2O (1.0% heptafluorobutyric acid HFBA) 2:2:1:5 (v/v/v/v) in reversed phase mode was used for preliminary purifi...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 21, 2018 Category: Drugs & Pharmacology Source Type: research

Dispersive magnetic solid phase extraction exploiting magnetic graphene nanocomposite coupled with UHPLC-PDA for simultaneous determination of NSAIDs in human plasma and urine
Publication date: Available online 16 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Vincenzo Ferrone, Maura Carlucci, Valeria Ettorre, Roberto Cotellese, Paola Palumbo, Antonella Fontana, Gabriella Siani, Giuseppe CarlucciAbstractA novel, rapid, simple graphene/Fe3O4 based dispersive magnetic solid phase extraction was developed for the simultaneous separation/preconcentration and determination of non steroidal anti-inflammatory drugs with ultra high performance liquid chromatography coupled with photodiode array detection. Several parameters influencing the extraction efficiency of the inv...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 21, 2018 Category: Drugs & Pharmacology Source Type: research

A Rapid, Simple, and High-throughput UPLC-MS/MS Method for Simultaneous Determination of Bioactive Constituents in Salvia Miltiorrhiza with Positive/Negative Ionization Switching
Publication date: Available online 16 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Hung-Yu Lin, Ting-Sian Lin, Han-Ju Chien, Yu-Min Juang, Chao-Jung Chen, Chang-Sheng Wang, Chien-Chen LaiAbstractThe dried root of Danshen (Salvia miltiorrhiza) is a Chinese medicine that has been used in traditional treatments for more than 2,000 years in Asia. It has also been used to treat cardiovascular diseases for decades. The goal of this study was to develop a new, fast, and simple ultrahigh-performance liquid chromatography tandem mass spectrometry (UPLC–MS/MS) analytical method for the analysi...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 21, 2018 Category: Drugs & Pharmacology Source Type: research

Precipitation behavior of pioglitazone on the particle surface of hydrochloride salt in biorelevant media
Publication date: Available online 16 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Taiga Uekusa, Kiyohiko SuganoAbstractThe purpose of the present study was to investigate the precipitation of a drug on the particle surface of its salt in biorelevant media. Pioglitazone (PIO, weak base, pKa = 5.8) was used as a model drug. The crystal particles of PIO hydrochloride (PIO HCl) were immobilized between two slide glasses. A biorelevant medium was penetrated between the slide glasses under a polarized light microscope. Crystalline precipitates appeared on the surface of PIO HCl wi...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 21, 2018 Category: Drugs & Pharmacology Source Type: research

Recent development and trends in sample extraction and preparation for mass spectrometric analysis of nucleotides, nucleosides, and proteins
Publication date: Available online 17 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Tadashi Hayama, Kaname OhyamaAbstractThis review describes the recent developments in sample extraction and preparation techniques for mass spectrometric analysis of nucleotides, nucleosides, and proteins. Unique materials and techniques have been developed for highly selective extraction of nucleotides and nucleosides by solid-phase extraction strategies using various affinities. However, for proteins, the analysis of small-scale sections of diseased tissues (formalin-fixed, paraffin-embedded tissues) and t...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 21, 2018 Category: Drugs & Pharmacology Source Type: research

Modified glassy carbon electrode with Polydopamine - multiwalled carbon nanotubes for simultaneous electrochemical determination of biocompounds in biological fluids
In this study, a simple modified glassy carbon electrode with Polydopamine-multiwalled carbon nanotubes (GCE/PDA-MWCNTs) is described for selective and sensitive simultaneous voltammetric determination of dopamine (DA), acetaminophen (AC), and xanthine (XN). A detailed investigation by field emission scanning electron microscopy, Fourier transform infrared spectroscopy and electrochemistry methods such as cyclic voltammetry (CV), electrochemical impedance spectroscopy, differential pulse voltammetry (DPV) and chronoamperometry are performed in order to elucidate the preparation process and properties of the GCE/ PDA-MWCNTs...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 21, 2018 Category: Drugs & Pharmacology Source Type: research

LC–MS/MS assay for the determination of a novel D-peptide antagonist of CXCR4 in rat plasma and its application to a preclinical pharmacokinetic study
Publication date: Available online 17 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Yinsong Zhu, Shu Yang, Juan Wang, Yujia Mao, Yan Xu, Jing An, Ziwei HuangAbstractDV1 is a potent and selective D-peptide antagonist of CXCR4 and being developed as a novel drug candidate molecule. For preclinical pharmacokinetic study of DV1, we established an efficient and reliable liquid chromatography coupled to tandem mass spectrometric (LC–MS/MS) method for the assay of DV1 in rat plasma. Plasma samples were acidified by formic acid and then their protein content precipitated by acetonitrile. Samp...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 21, 2018 Category: Drugs & Pharmacology Source Type: research

Characterization of a new illicit phosphodiesterase-type-5 inhibitor identified in the softgel shell of a dietary supplement
Publication date: Available online 18 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Takahiro Doi, Kazunaga Takahashi, Midori Yamazaki, Akiko Asada, Akihiro Takeda, Kyohei Kiyota, Takaomi Tagami, Yoshiyuki Sawabe, Tetsuo YamanoAbstractA new sildenafil analog has been identified in the softgel shell of a dietary supplement. The compound was investigated by UV spectroscopy and high-resolution MS analysis, leading to the proposed structure 1-methyl-5-{5-[2-(4-methylpiperazin-1-yl)acetyl]-2-propoxyphenyl}-3-propyl-1,6-dihydro-7H-pyrazolo[4,3-d]pyrimidin-7-one. A synthetic reference compound with...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 21, 2018 Category: Drugs & Pharmacology Source Type: research

A Functional In Vitro Assay for Screening Inhibitors of STAT5B Phosphorylation
Publication date: Available online 18 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Elvin D. de Araujo, Pimyupa Manaswiyoungkul, Fettah Erodgan, Abdul K. Qadree, Diana Sina, Gary Tin, Krimo Toutah, Karen Yuen, Patrick T. GunningAbstractInhibition of STAT phosphorylation is recognized as a viable therapeutic strategy for disrupting tumorigenesis. Constitutive STAT phosphorylation is found with high frequency in a number of primary tumor types, while non-cancer cells exhibit low basal activity, providing an exploitable therapeutic window. STAT activation involves phosphorylation of the SH2 do...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 21, 2018 Category: Drugs & Pharmacology Source Type: research

In vitro binding interaction of atorvastatin with calf thymus DNA: multispectroscopic, gel electrophoresis and molecular docking studies
Publication date: Available online 18 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Zeinab Mirzaei-KalarAbstractThe interaction of atorvastatin with calf thymus DNA (CT-DNA) was investigated in vitro under simulated physiological conditions by using absorption and emission spectroscopy, viscosity measurements, gel electrophoresis and molecular docking studies. Analysis of UV-Visible absorbance spectra indicates the formation of complex between atorvastatin and CT-DNA, and obtained binding constant (Kb = 8.2×104 L. mol-1) is comparable to groove binder drugs. Sligh...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 21, 2018 Category: Drugs & Pharmacology Source Type: research

A new method for the determination of total and released docetaxel from docetaxel-entrapped core-crosslinked polymeric micelles (CriPec®) by LC-MS/MS and its clinical application in plasma and tissues in patients with various tumours
In conclusion the newly developed assay met the required standards for validation and was applied successfully to support pharmacokinetic analysis in both serum and tissue in patients treated with Cripec®. (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - August 21, 2018 Category: Drugs & Pharmacology Source Type: research

Identification of naringin metabolites mediated by human intestinal microbes with stable isotope-labeling method and UFLC-Q-TOF-MS/MS
In this study, we aim of systematically investigating the biotransformation of naringin mediated by human intestinal microbes through applying stable isotope-labeling method. [2',3',5',6'-D4]naringin was synthesized and incubated anaerobically with human gut microbes. A total of 13 microbial metabolites were detected and identified by UFLC-Q-TOF-MS/MS, among which 5 were reported for the first time. Furthermore, the proposed metabolic pathway revealed that naringin went through extensive phase I metabolism in human intestinal microbes. Of note, diverse metabolic profiles of naringin among human participants were obtained, ...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 21, 2018 Category: Drugs & Pharmacology Source Type: research

Bioanalytical liquid chromatography-tandem mass spectrometric assay for the quantification of the ALK inhibitors alectinib, brigatinib and lorlatinib in plasma and mouse tissue homogenates
Publication date: Available online 19 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Rolf W. Sparidans, Wenlong Li, Alfred H. Schinkel, Jan H.M. Schellens, Jos H. BeijnenAbstractSeveral second and third generation ALK inhibitors have been introduced in recent years. A bioanalytical assay for simultaneous quantification of alectinib, brigatinib, and lorlatinib was developed and validated for human plasma. The method was also partially validated for diluted mouse plasma and tissue homogenates of brain, liver, kidney, and spleen. Samples (40 µl) were pretreated in a 96-well plate by pro...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 21, 2018 Category: Drugs & Pharmacology Source Type: research

Falsification of biotechnology drugs: Current dangers and/or future disasters?
Publication date: Available online 20 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Steven Janvier, Bart De Spiegeleer, Celine Vanhee, Eric Deconinck (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - August 21, 2018 Category: Drugs & Pharmacology Source Type: research

Use of thermal and non thermal techniques for assessing compatibility between mirtazapine and solid lipids
Publication date: Available online 20 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Randeep Kaur, V.R. SinhaAbstractThe present investigation was aimed at the evaluation of possible interactions between mirtazapine and selected solid lipids that are commonly used to develop solid lipid nanoparticles (SLNs) and nanostructured lipidic carriers (NLCs). The solids lipids explored were palmitic acid, stearic acid, glycerylmonostearate, cutina CPPH, sterotex NF, gelucire 50/13, hydrogenated castor oil and compritol 888 ATO. The techniques used were Differential Scanning Calorimetry (DSC), Fourier...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 21, 2018 Category: Drugs & Pharmacology Source Type: research

Nontargeted metabolomics approach to determine metabolites profile and antioxidant study of Tropical Almond (Terminalia catappa L.) fruit peels using GC-QTOF-MS and LC-QTOF-MS
The objective of the present study was to identify the metabolome pattern and study the biological efficacy of Almond (Terminalia catappa L.) peels. In the present study, metabolite profiling was carried out using GC-QTOF-MS and LC-QTOF-MS techniques, from the potent extract showed highest antioxidant efficacy. Antioxidant efficacy of the various extracts derived by six different extraction methods was assessed by different assays viz. 2,2-diphenyl-1-picrylhydrazyl, superoxide, 2,2′-Azino-bis-(3-ethyl)benzothiazoline)-6-sulfonic acid diammonium salt radical cation inhibition assays and ferric reducing antioxidant pow...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 21, 2018 Category: Drugs & Pharmacology Source Type: research

Novel electrophoretic acetonitrile-based stacking for sensitive monitoring of the antiepileptic drug perampanel in human serum
Publication date: 25 October 2018Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 160Author(s): Petr Tůma, Miroslava Bursová, Blanka Sommerová, Rachel Horsley, Radomír Čabala, Tomáš HložekAbstractPerampanel is a novel antiepileptic drug used in paediatric patients. Existing methods that determine serum perampanel are of limited practicability. We developed a novel capillary electrophoresis (CE) method using a new version of acetonitrile stacking for on-line sample pre-concentration, and fluorescence detection (FD). CE separations were performed in a fused-silica capill...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 17, 2018 Category: Drugs & Pharmacology Source Type: research

Rapid authentication and differentiation of herbal medicine using 1H NMR fingerprints coupled with chemometrics
In conclusion, the developed method is an accurate, sensitive and rapid authentication of Polygoni Multiflori Radix, which could be a useful tool for rapid authentication and quality control of herbal medicines. (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - August 14, 2018 Category: Drugs & Pharmacology Source Type: research

Paper-based analytical devices prepared with polycaprolactone printing and their application in the activity determination of mulberry extracts
Publication date: Available online 11 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Guo Shangxin, Shao Jingyuan, Gong XingchuAbstractA new method for preparing paper-based analytical devices (PADs) with the assistance of 3D printing technology was presented. Polycaprolactone was used as the 3D printing material to form hydrophobic barriers. First, polycaprolactone was 3D printed on filter paper. The polycaprolactone-modified paper was then prepared by heating. Experiments on α-glucosidase inhibition activity were carried out in the hydrophilic circles on the modified paper. Color data...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 12, 2018 Category: Drugs & Pharmacology Source Type: research

Recent advancements, challenges, and practical considerations in the mass spectrometry-based analytics of protein biotherapeutics: a viewpoint from the biosimilar industry
Publication date: Available online 11 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Viktor Háda, Attila Bagdi, Zsolt Bihari, Sarolta Baginé Timári, Ádám Fizil, Csaba SzántayAbstractThe extensive analytical characterization of protein biotherapeutics, especially of biosimilars, is a critical part of the product development and registration. High-resolution mass spectrometry became the primary analytical tool used for the structural characterization of biotherapeutics. Its high instrumental sensitivity and methodological versatility made it possible t...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 12, 2018 Category: Drugs & Pharmacology Source Type: research

Untargeted rat brain metabolomics after oral administration of a single high dose of cannabidiol
Publication date: Available online 11 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Cinzia Citti, Federica Palazzoli, Manuela Licata, Antonietta Vilella, Giuseppina Leo, Michele Zoli, Maria Angela Vandelli, Flavio Forni, Barbara Pacchetti, Giuseppe CannazzaAbstractCannabidiol (CBD), for long time considered as a minor cannabinoid of Cannabis sativa, has recently gained much attention due to its antioxidant, anti-inflammatory, analgesic and anticonvulsant properties. A liquid chromatography coupled to mass spectrometry based method was developed for the quantitative determination of CBD and ...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 12, 2018 Category: Drugs & Pharmacology Source Type: research

Identification of hepatotoxic and nephrotoxic potential markers of triptolide in mice with delayed-type hypersensitivity
In this study, a feasible strategy using HPLC-HRMS method combined with multivariate statistical analysis to discover toxic potential markers of TP was developed. TP was used to treat a DTH mouse model at a therapeutic dose (45μg/kg) and toxic dose (900 μg/kg). The metabolic profiles of the liver, kidney and plasma were characterized by HPLC-Q/TOF MS. Significant differences in the metabolite profiles of the liver, kidney and plasma existed between the toxic and therapeutically dosed mice. Forty-six metabolites were identified and 27 of them may be related to toxicity based on a structure-toxicity prediction model. U...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 11, 2018 Category: Drugs & Pharmacology Source Type: research

Kineret Protein Solution Survives Ten Years
Publication date: Available online 10 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Yinghua Peng, Xiaohui WangAbstractProteins are labile and their shelf lives are usually months to few years. Herein we report that human interleukin-1 receptor antagonist (IL-1Ra) solution survives ten years. This provides an interesting case that protein biopharmaceutical aqueous solution could be stored for a decade without losing its quality, which could meet the national security needs for 10 years shelf life of biopharmaceuticals. (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - August 11, 2018 Category: Drugs & Pharmacology Source Type: research

Novel controllable hydrophilic thermo-responsive molecularly imprinted resin adsorbent prepared in water for selective recognition of alkaloids by thermal-assisted dispersive solid phase extraction
Publication date: Available online 10 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Weiyang Tang, Kyung Ho RowAbstractA novel controllable hydrophilic thermo-responsive molecularly imprinted resin (T-MIR) with a switchable zipper-like architecture was synthesized in the aqueous phase and applied to the selective recognition and extraction of alkaloids by positive temperature regulation. In this synthesis process of T-MIR, 2-acrylamide-2- methylpropanesulfonic acid (AMPS) and acrylamide (AAm) were coupled as zipper-like thermo-responsive monomers, resorcinol, and melamine as hydrophilic mono...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 11, 2018 Category: Drugs & Pharmacology Source Type: research

Characterization of phytochemical components of Ferula halophila extracts using HPLC-MS/MS and their pharmacological potentials: A multi-functional insight
Publication date: Available online 10 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Gokhan Zengin, Ahmet Uysal, Alina Diuzheva, Erdogan Gunes, József Jekő, Zoltán Cziáky, Carene Marie Nancy Picot-Allain, Mohamad Fawzi MahomoodallyAbstractThe inhibitory action of F. halophila extracts (acetone, chloroform, and methanol) against key enzymes linked to diabetes (α-amylase, α-glucosidase), cognitive enhancing functions (acetyl cholinesterase (AChE), butyryl cholinesterase (BChE)), and hyperpigmentation (tyrosinase) was assessed. The mutagenic/antimutagenic activ...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 11, 2018 Category: Drugs & Pharmacology Source Type: research

Quantitation of residual host cell DNA in protaminesulfate drug product by qPCR
Publication date: 25 October 2018Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 160Author(s): Cynthia Sommers, Barry Rosenzweig, Lida Oum, Karol Thompson, David A. KeireAbstractProtamine sulfate (PS) is an FDA approved drug used to reverse heparin-induced anticoagulation in patients. Protamine sulfate is a mixture of primarily four ∼4 kDa arginine-rich cationic polypeptide chains derived from chum (Oncorhynchus keta) salmon sperm. Because the presence of residual host cell salmon DNA (resDNA) in PS drug product can pose safety concerns, processing steps during PS manufacturing are designed to ...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 11, 2018 Category: Drugs & Pharmacology Source Type: research

Proteomic analysis of evodiamine-induced cytotoxicity in thyroid cancer cells
This study demonstrated 77 protein features that were significantly changed in protein expression and revealed evodiamine-induced cytotoxicity in thyroid cancer cells involves dysregulation of protein folding, cytoskeleton, cytoskeleton regulation and transcription control. Our work shows that this combined proteomic strategy provides a rapid method to study the molecular mechanisms of evodiamine-induced cytotoxicity in thyroid cancer cells. The identified targets may be useful for further evaluation as potential targets in thyroid cancer therapy.Graphical abstract (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - August 10, 2018 Category: Drugs & Pharmacology Source Type: research

Investigation of the thermal shift assay and its power to predict protein and virus stabilizing conditions
Publication date: Available online 9 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Dora Sviben, Branimir Bertoša, Andrea Hloušek-Kasun, Dubravko Forcic, Beata Halassy, Marija BrglesAbstractProtein thermal shift assay (TSA) has been extensively used in investigation of protein stabilization (for protein biopharmaceutics stabilization, protein crystallization studies or screening of recombinant proteins) and drug discovery (screening of ligands or inhibitors). This work aimed to analyze thermal shift assay results in comparison to protein polymerization (multimerization and aggr...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 10, 2018 Category: Drugs & Pharmacology Source Type: research

Saliva of patients affected by salivary gland tumour: An NMR metabolomics analysis
Publication date: Available online 9 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Manuela Grimaldi, Angelica Palisi, Giuseppina Rossi, Ilaria Stillitano, Francesco Faiella, Paola Montoro, Manuela Rodriquez, Remo Palladino, Anna Maria D’Ursi, Rocco RomanoAbstractCancers affecting the salivary glands have been an increasing incidence. Salivary gland cancer is not detected until it reaches an advanced stage, which would generally result in a poor prognosis and survival rate. Therefore, early detection as well as the screening of high risk populations with precancerous lesions remains an...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 9, 2018 Category: Drugs & Pharmacology Source Type: research

Combing metabolomics with bioanalysis methods to study the antitumor mechanism of the new acridone derivative 8q on CCRF-CEM cells: 8q induced mitochondrial-mediated apoptosis and targeted the PI3K/AKT/FOXO1 pathway
In conclusion, these findings demonstrate that 8q can induce mitochondrial lesions and promote mitochondrial-mediated pathway apoptosis by regulating the expression of Bcl-2 family proteins and inhibiting the activity of the PI3K/AKT/FOXO1 signaling pathway.Graphical abstractThe novel acridone derivative, 8q, can induce mitochondrial lesions and promote mitochondrial-mediated pathway apoptosis by regulating the expression of Bcl-2 family proteins and inhibiting the activity of the PI3K/AKT/FOXO1 signaling pathway to against CCRF-CEM cells. (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - August 9, 2018 Category: Drugs & Pharmacology Source Type: research

Analytical aspects of sunitinib and its geometric isomerism towards therapeutic drug monitoring in clinical routine
Publication date: Available online 8 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Bianca Posocco, Mauro Buzzo, Luciana Giodini, Sara Crotti, Sara D’Aronco, Pietro Traldi, Marco Agostini, Elena Marangon, Giuseppe ToffoliAbstractSunitinib malate, an oral multi-targeted tyrosine kinase inhibitor approved for the treatment of metastatic renal cell carcinoma, gastrointestinal stromal tumor, and well-differentiated pancreatic neuroendocrine tumors, has been identified as a potential candidate for therapeutic drug monitoring approach. Nevertheless, the development of an analytical assay sui...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 9, 2018 Category: Drugs & Pharmacology Source Type: research

The application of on-line two-dimensional liquid chromatography (2DLC) in the chemical analysis of herbal medicines
Publication date: Available online 8 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Shuai Ji, Shuang Wang, Haishan Xu, Zhenyu Su, Daoquan Tang, Xue Qiao, Min YeAbstractHerbal medicines are complicated chemical systems containing hundreds of small molecules of various polarities, structural types, and contents. Thus far, the chromatographic separation of herbal extracts is still a big challenge. Two-dimensional liquid chromatography (2DLC) has become an attractive separation tool in the past few years. Particularly, a lot of attention has been paid to on-line 2DLC. In this review, we aim to g...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 9, 2018 Category: Drugs & Pharmacology Source Type: research

Rapid authentication and differentiation of herbal medicine using 1H-NMR fingerprints coupled with chemometrics
In conclusion, the developed method is an accurate, sensitive and rapid authentication of Polygoni Multiflori Radix, which could be a useful tool for rapid authentication and quality control of herbal medicines. (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - August 8, 2018 Category: Drugs & Pharmacology Source Type: research

Advancement in the chemical analysis of Paeoniae Radix (Shaoyao)
Publication date: Available online 7 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Binjun Yan, Menglan Shen, Jinyang Fang, Danni Wei, Luping QinAbstractPaeoniae Radix Alba (baishao or white peony root) and Paeoniae Radix Rubra (chishao or red peony root) are two highly valuable traditional Chinese medicines (TCMs) usually indicated for painful conditions, menstrual disorders and viral infections. These two TCMs are collectively referred to as shaoyao (Paeoniae Radix) due to their close origins and similar chemical compositions. Modern research indicates that monoterpene glycosides, polyphen...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 8, 2018 Category: Drugs & Pharmacology Source Type: research

Novel electrophoretic acetonitrile-based stacking for sensitive monitoring of the novel antiepileptic drug perampanel in human serum
Publication date: Available online 7 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Petr Tůma, Miroslava Bursová, Blanka Sommerová, Rachel Horsley, Radomír Čabala, Tomáš HložekAbstractPerampanel is a novel antiepileptic drug used in paediatric patients. Existing methods that determine serum perampanel are of limited practicability. We developed a novel capillary electrophoresis (CE) method using a new version of acetonitrile stacking for on-line sample pre-concentration, and fluorescence detection (FD). CE separations were performed in a fused-silica cap...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 8, 2018 Category: Drugs & Pharmacology Source Type: research

Silver Nanoparticles Decorated Polyaniline Nanocomposite Based Electrochemical Sensor for the Determination of Anticancer Drug 5-Fluorouracil
Publication date: Available online 7 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Fatemeh Modarres Zahed, Behnaz Hatamluyi, Farnaz Lorestani, Zarrin Es’haghiAbstractA highly efficient electrochemical sensor for the analysis of anticancer drug 5-fluorouracil (5-FU), is fabricated based on silver nanoparticles-polyaniline nanotube (AgNPs@PANINTs). AgNPs@PANINTs nanocomposite has been synthesized by a simple one-step method. Synthesized AgNPs@PANINTs nanocomposite was studied by Fourier transform infrared spectrometry, Scanning Electron Microscopy and Energy Dispersive X-ray. The fabric...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 8, 2018 Category: Drugs & Pharmacology Source Type: research

Evaluation of extraction and normalisation strategies for the analysis of lipids in placental vessels
Publication date: Available online 7 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Katrin N. Sander, Daniel P. Hay, Fiona Broughton-Pipkin, Raheela N. Khan, David A. BarrettAbstractThe analysis of lipids in tough or fibrous biological tissues can be challenging due to difficulties in obtaining a representative sample following homogenisation of the tissue. Furthermore, the choice of normalisation method can have a major effect on the quality of quantitative results. Therefore, a range of mechanical homogenisation techniques and normalisation strategies were evaluated for application to huma...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 8, 2018 Category: Drugs & Pharmacology Source Type: research

Diagnostic Absolute Configuration Determination of Clavulanate Potassium: A Comprehensive Investigation of Chiroptical Spectroscopies and Theoretical Calculations
Publication date: Available online 7 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Zhen Li, Linwei Li, Yi Zheng, Cong Chen, Tiemin SunAbstractClavulanate potassium (CP) is a classic β-lactamase inhibitor, which is extensively used as a combination drug in antibiotics therapy. As a chiral drug, CP is the only effective stereoisomer revealing prominent activities. To determine the chirality of CP efficiently, we provide an accurate and convenient method based on the comprehensive investigation of circular dichroism spectra and theoretical calculations. The results demonstrate two disting...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 8, 2018 Category: Drugs & Pharmacology Source Type: research

LC-MS Analysis of Key Components of the Glutathione Cycle in Tissues and Body Fluids from Mice with Myocardial Infarction
Publication date: Available online 7 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Andres Gil, Atze van der Pol, Peter van der Meer, Rainer BischoffAbstractOxidative stress is suggested to play an important role in several pathophysiological conditions. A recent study showed that decreasing 5-oxoproline (pyroglutamate) concentration, an important mediator of oxidative stress, by over-expressing 5-oxoprolinase, improves cardiac function post-myocardial infarction in mice. The aim of the current study is to gain a better understanding of the role of the glutathione cycle in a mouse model of m...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 8, 2018 Category: Drugs & Pharmacology Source Type: research