Chemical markers for quality control of bran-fried sulfur-fumigated Paeoniae Radix Alba
Publication date: Available online 5 July 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Ya-Yun Xu, Fang Long, Ye-Qing Zhang, Jin-Di Xu, Ming Kong, Song-Lin LiAbstractIn traditional Chinese medicine practice, crude herbs are often subjected to traditional processing (Paozhi in Chinese) for a special medicinal purpose. Bran-frying is one of processing methods for Paeoniae Radix Alba (PRA). Previous studies found that paeoniflorin and paeoniflorin sulfonate, a principle bioactive compound and its sulfur-fumigation induced characteristic sulfur-containing derivative, could be used together with sulfur...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Discovery of quality control markers from traditional Chinese medicines by fingerprint-efficacy modeling: Current status and future perspectives
Publication date: Available online 8 July 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Cai Zhang, Xian Zheng, Hui Ni, Ping Li, Hui-Jun LiAbstractQuality control (QC) is crucial for ensuring the safety and effectiveness of traditional Chinese medicines (TCMs). Due to extremely complicated phytochemical matrices and characteristic mode of “multi-component, multi-target, integrated adjustment”, discovering of QC markers from TCMs is a big challenge. Fingerprint-efficacy (FE) modeling method is currently proposed as an effective and reasonable attempt. This review summarizes the methodolo...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Development and validation of a simple chromatographic method for simultaneous determination of clindamycin phosphate and rifampicin in skin permeation studies
Publication date: Available online 10 July 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Maira N. Pereira, Breno N. Matos, Tais Gratieri, Marcilio Cunha-Filho, Guilherme M. GelfusoAbstractRifampicin (RIF) and clindamycin phosphate (CDM) are the main drugs currently used in combination to treat severe infectious diseases in hair follicles. This work describes a simple, rapid and sensitive method for simultaneous analysis of RIF and CDM in the different skin layers using high performance liquid chromatography (HPLC). The efficient chromatographic separation of CDM and RIF was succeeded using a C18 c...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

New developments and possibilities of wide-pore superficially porous particle technology applied for the liquid chromatographic analysis of therapeutic proteins
Publication date: 5 September 2018Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 158Author(s): Balázs Bobály, Jean-Luc Veuthey, Davy Guillarme, Szabolcs FeketeAbstractThis review paper discusses the success of columns packed with superficially porous particles (SPP) in liquid chromatography for the analysis of peptides and proteins. First, it summarizes the history of SPP, including the development of different SPP generations from particles of 50 μm to sub-2 μm. It also critically discusses the improved kinetic performance of SPP particles in comparison to fully porous particles....
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Quantification of nucleotides and their sugar conjugates in biological samples: Purposes, instruments and applications
Publication date: 5 September 2018Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 158Author(s): Xuan Qin, Xin WangAbstractNucleotides and their sugar conjugates are fundamental molecules in life, participating in processes of DNA/RNA composition, cell wall build-up, glycosylation reactions, and signal conduction. Therefore, the quantification of these compounds in biological samples significantly benefits the understanding of their functions. However, nucleotides and nucleotide sugars are extremely hydrophilic, causing bad retention and peak symmetry on regular C18 chromatographic columns. To solve this p...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Recent advances in the capillary electrophoresis analysis of antibiotics with capacitively coupled contactless conductivity detection
Publication date: 5 September 2018Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 158Author(s): Prasanta Paul, Cari Sänger-van de Griend, Erwin Adams, Ann Van SchepdaelAbstractThis review describes briefly the high rate of counterfeiting of antimicrobial drugs with focus upon its immediate health consequences. The major part of this review encompasses accounts of the improvements achieved in the domain of miniaturization of capillary electrophoresis with capacitively coupled contactless conductivity detection (CE-C4D). The application of this principle into the development of portable devices as well...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Simultaneous determination of tryptophan, kynurenine, kynurenic acid and two monoamines in rat plasma by HPLC-ECD/DAD
Publication date: 5 September 2018Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 158Author(s): Ting-ting Du, Ting Cui, Hong-mei Qiu, Nian-ru Wang, Dan Huang, Xin-hui JiangAbstractA high-performance liquid chromatography method with a diode array and an electrochemical detection (HPLC-ECD/DAD) was developed to determine the levels of tryptophan (TRP), kynurenine (KYN), kynurenic acid (KYA), 5-hydroxytryptamine (5-HT) and 5-hydroxyindoleacetic acid (5-HIAA) in rat plasma. The prepared samples were separated on a BDS column (4.6 mm × 250 mm, 5 mm) with column oven temperature of 25 °C....
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Bioanalysis of free and liposomal Amphotericin B in rat plasma using solid phase extraction and protein precipitation followed by LC-MS/MS
In this study, an original strategy was developed to separately determine F-AMB and L-AMB in rat plasma using LC–MS/MS. F-AMB was analyzed after separation by solid phase extraction, total AMB (T-AMB) was determined after protein precipitation and L-AMB was determined by difference. The method was fully validated. Calibration curves were linear in the ranges 0.7–120 μg/mL for T-AMB and 0.2–20 μg/mL for F-AMB. Accuracy and precision results were within acceptable variability limits, recoveries were consistent and reproducible, matrix effects were insignificant and analytes were stable under all t...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Efficient extraction of proanthocyanidin from Ginkgo biloba leaves employing rationally designed deep eutectic solvent-water mixture and evaluation of the antioxidant activity
Publication date: 5 September 2018Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 158Author(s): Jun Cao, Luyao Chen, Mohan Li, Fuliang Cao, Linguo Zhao, Erzheng SuAbstractProanthocyanidin (PAC) holds strong free radicals scavenging ability and is widely used as oxygen free radical scavenger. Huge demand, safety and economic aspect are forcing the PAC extraction to explore new resource and greener solvent. An efficient extraction method of PAC from Ginkgo biloba leaves was established by employing deep eutectic solvent (DES)-water mixture. After a rational design, a DES-water mixture (named as Ch-M55) was ...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Sandwich pair nanobodies, a potential tool for electrochemical immunosensing serum prostate-specific antigen with preferable specificity
In this study, a sandwich pair of nanobodies (Nbs) (i.e., Nb2 and Nb40) against PSA surface antigen was obtained from an alpaca-derived immune phage display library. A sandwich-type immunosensor for the sensitive and selective detection of PSA in serum samples was ingeniously designed based on the pair of Nbs. The small size of Nb40 allowed high capture densities on the surface of reduced graphene oxide (rGO) nanocomposed with massive Au nanoparticles (rGO@AuNPs), which significantly improved the conductivity and provided a large area to anchor many primary antibodies. The secondary antibody Nb2 fused with streptavidin -bi...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

On-line electrochemistry/electrospray ionization mass spectrometry (EC-ESI-MS) system for the study of nucleosides and nucleotides oxidation products
Publication date: 5 September 2018Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 158Author(s): Sylwia Studzińska, Lena Siecińska, Bogusław BuszewskiAbstractThe main aim of present investigation was to study the oxidation products of nucleosides and nucleotides with the use of on-line electrochemistry/electrospray ionization mass spectrometry (EC-ESI-MS) system. The conditions applied in the system were optimized in complex manner, involving study of the impact of working electrodes or sample solvent on the oxidation of tested compounds and their ionization in mass spectrometry. Finally 5 mM of ammon...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Gold nanoparticles coupled with graphene quantum dots in organized medium to quantify aminoglycoside anti-biotics in yellow fever vaccine after solid phase extraction using a selective imprinted polymer
Publication date: 5 September 2018Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 158Author(s): Carlos A.T. Toloza, Joseany M.S. Almeida, Sarzamin Khan, Yasmin G. dos Santos, Andrea R. da Silva, Eric C. Romani, Dunieskys G. Larrude, Fernando L. Freire, Ricardo Q. AucélioAbstractThe determination of kanamycin sulfate was made indirectly by measuring its effect on photoluminescent amino functionalized graphene quantum dots (GQDs-amino) associated with gold nanoparticles (AuNPs) that were produced by the reduction of AuCl4 with NaBH4 in an aqueous dispersion of GQDs-amino (obtained by the pyrolysis of...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Interval importance index to select relevant ATR-FTIR wavenumber Intervals for falsified drug classification
Publication date: 5 September 2018Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 158Author(s): A. Kahmann, M.J. Anzanello, F.S. Fogliatto, W.A. Chaovalitwongse, M.C.A. Marcelo, M.F. Ferrão, R.S. Ortiz, K.C. MariottiAbstractThe commerce of falsified drugs has substantially grown in recent years due to facilitated access to technologies needed for copying authentic pharmaceutical products. Attenuated Total Reflectance coupled with Fourier Transform Infrared (ATR-FTIR) spectroscopy has been successfully employed as an analytical tool to identify falsified products and support legal agents in interrup...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Quantitation of polymorphic impurity in entecavir polymorphic mixtures using powder X-ray diffractometry and Raman spectroscopy
Publication date: 5 September 2018Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 158Author(s): Yanlei Kang, Zhanying Shao, Qiang Wang, Xiurong Hu, Dongdong YuAbstractEntecavir was used for the treatment of chronic hepatitis B through inhibiting hepatitis B virus. The anhydrous form of entecavir (ENT-A) often appeared as impurity polymorph in the manufacturing process of entecavir monohydrate (ENT-H) such as granulation, drying and compression. Since different crystal forms might affect drug bioavailability and therapeutic effect, it was vital to control the ENT-A content of the drug product. The work aim...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Metabolomics and antioxidant activity of the leaves of Prunus dulcis Mill. (Italian cvs. Toritto and Avola)
Publication date: 5 September 2018Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 158Author(s): Alfredo Bottone, Paola Montoro, Milena Masullo, Cosimo Pizza, Sonia PiacenteAbstractPrunus dulcis leaves have been reported to exert some biological activity, in particular potent free radical-scavenging capacity, but so far there is limited information on their chemical composition. With the aim to achieve deep insight on the chemical constituents of the leaves of P. dulcis cultivars “Toritto” and “Avola”, the most appreciated in Italy, an approach based on liquid chromatography-mass sp...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Degradation caused by incompatibility between sodium stearyl fumarate (PRUV) and AZD7986 in the drug product
Publication date: 5 September 2018Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 158Author(s): Jufang Wu Ludvigsson, Håkan Wikström, Thomas Andersson, Per-Ola NorrbyAbstractDuring compatibility study of the AZD7986 project, a peak of 3 area% at the tail (RRT 1.03) of the active pharmaceutical ingredient (API) was discovered for all tablets containing sodium stearyl fumarate (PRUV) under humid condition (e.g. 50 °C/75% RH), regardless of choice of disintegrant or filler combination. The degradant was needed to be identified to understand the corresponding reaction mechanism and help the f...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Study of degradation behaviour of montelukast sodium and its marketed formulation in oxidative and accelerated test conditions and prediction of physicochemical and ADMET properties of its degradation products using ADMET Predictor™
This study was aimed to understand the degradation behaviour of the drug in different oxidative media containing hydrogen peroxide, AIBN, Fe3+, Fenton’s reagent and O2 environment under normal laboratory light conditions. The degradation behaviour of the drug was also evaluated in solid sate under ICH recommended accelerated stability condition of 40 °C/75% RH to correlate with the degradation products (DPs) formed in a solid oral formulation. A total of nine DPs (MTK 1 to MTK 9) were formed from both the drug substance and the marketed tablet formulation on storage under controlled oxygen environment in normal...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Identification and quantification of ethylene oxide in sterilized medical devices using multiple headspace GC/MS measurement
Publication date: 5 September 2018Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 158Author(s): Pascal Gimeno, Marie-Laure Auguste, Vagn Handlos, Anne Mette Nielsen, Stephan Schmidt, Nelly Lassu, Martin Vogel, Antonius Fischer, Charlotte Brenier, Françoise DuperrayAbstractThis manuscript, based on the ISO 10993-7 approach, describes a multiple HS-GC measurement of residual EO present in sterilized plastic samples. The quantification of EO is done, according to the ISO standard, by addition of EO amounts extracted for each repeated extraction. During the method development, the specificity of the de...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Multiwavelength UV-metric and pH-metric determination of the multiple dissociation constants of the lesinurad
Publication date: 5 September 2018Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 158Author(s): Milan Meloun, Aneta Čápová, Lucie Pilařová, Tomáš PekárekAbstractPotentiometric and spectrophotometric pH-titrations of the lesinurad for three consecutive dissociation constants determination were compared. Lesinurad is a selective inhibitor of uric acid reabsorption as part of a combination of medicines to treat high levels of uric acid in blood, also called hyperuricemia. Nonlinear regression of the pH-spectra with REACTLAB and SQUAD84 and of the pH-titration curve...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Study on the stability control strategy of Triphala solution based on the balance of physical stability and chemical stabilities
Publication date: 5 September 2018Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 158Author(s): Hao-zhou Huang, Sheng-yu Zhao, Xiu-mei Ke, Jun-zhi Lin, Shu-sen Huang, Run-chun Xu, Hong-yan Ma, Yi Zhang, Li Han, Ding-kun ZhangAbstractTriphala is a well-known prescription in Indian Ayurveda and TCM medicine for its great effect on gingivitis and hyperlipidemia. However, its solution is unstable for the containing of excessive polyphenol, leading to the production of sediment in the short term and the decrease of efficacy. Based on the analysis of sediment formation, a novel control strategy is proposed. To ...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Comparison of multivariate data analysis techniques to improve glucose concentration prediction in mammalian cell cultivations by Raman spectroscopy
In this study, four mammalian cell cultivation runs were followed with Raman spectroscopy and the spectra were analysed quantitatively and qualitatively as well. The PCA analysis showed that one of the most dominant factors in the Raman spectra were the concentration of glucose, which strongly correlated with the score values of the eighth principal component. This observation further substantiated that Raman spectroscopy is an excellent tool for bioprocess monitoring and induced the test of the Multivariate Linear Regression (MLR), Principal Component Regression (PCR) and Partial Least Squares Regression (PLSR) algorithms...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Simultaneous determination of dextromepromazine and related substances 2-methoxyphenothiazine and levomepromazine sulfoxide in levomepromazine on a cellulose tris(4-methylbenzoate) chiral column
Publication date: 5 September 2018Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 158Author(s): Stephan Niedermeier, Iza Matarashvili, Bezhan Chankvetadze, Gerhard K.E. ScribaAbstractA high-performance liquid chromatography method for the determination of dextromepromazine, levomepromazine sulfoxide and 2-methoxyphenothiazine in levomepromazine samples was developed. The separation of the analytes was achieved within 10 min on a stationary phase containing cellulose tris(4-methylbenzoate) as chiral selector. The mobile phase consisted of 0.1% diethylamine in methanol with a flow rate of 1.0 mL/min. Th...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

A Quality by Design (QbD) approach to the development of a gradient high-performance liquid chromatography for the simultaneous assay of curcuminoids and doxorubicin from long-circulating liposomes
Publication date: 5 September 2018Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 158Author(s): Bianca Sylvester, Lucia Tefas, Laurian Vlase, Ioan Tomuţă, Alina PorfireAbstractThe present study highlights the advantages of using an Analytical Quality by Design (AQbD) approach to the optimization of a high-performance liquid chromatography method for the simultaneous assay of curcumin (CUR), demetoxycurcumin (DMC), bisdemetoxycurcumin (BDMC) and doxorubicin (DOX) co-encapsulated in long circulating liposomes. Within the QbD paradigm, the present study aimed to establish the method operable design region ...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Chemometrics-assisted study of the interconversion between the crystalline forms of nimodipine
Publication date: 5 September 2018Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 158Author(s): Natalia L. Calvo, Naira M. Balzaretti, Marina Antonio, Teodoro S. Kaufman, Rubén M. MaggioAbstractNimodipine (NIM) is a calcium channel-blocking agent, which in the solid state exhibits two crystalline modifications, Mode I and Mode II. The first one is a racemic mixture, while the second is a conglomerate. Because the drug has poor aqueous solubility and Mode I is twice as soluble as Mode II, the former is widely preferred for the development of pharmaceutical forms. In order to study the effect of ther...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Acetylcholinesterase-inhibitory activity of Iranian plants: Combined HPLC/bioassay-guided fractionation, molecular networking and docking strategies for the dereplication of active compounds
Publication date: 5 September 2018Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 158Author(s): Mahdi Abbas-Mohammadi, Mahdi Moridi Farimani, Peyman Salehi, Samad Nejad Ebrahimi, Ali Sonboli, Celine Kelso, Danielle SkropetaAbstractIn order to search for discovery of acetylcholinesterase (AChE) inhibitors, as a therapeutic strategy for treatment of the Alzheimer’s disease, twenty-five Iranian plants have been evaluated by an in vitro enzymatic Ellman method and molecular docking study. Each plant was successively extracted by n-hexane, ethyl acetate and methanol to obtain a total of 75 extracts. The ...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Simultaneous determination of parecoxib and its main metabolites valdecoxib and hydroxylated valdecoxib in mouse plasma with a sensitive LC–MS/MS method to elucidate the decreased drug metabolism of tumor bearing mice
In this study, a sensitive, rapid and specific LC–MS/MS method for quantification of PX, VX and OH-VX in the plasma of tumor bearing mouse was developed and validated. After protein precipitation, all the analytes were separated on an Agilent ZORBAX Extend-C18 HPLC column (2.1 × 100 mm, 3.5 μm) with gradient elution. The analytes were detected by an electrospray negative ionization mass spectrometry in the multiple reaction monitoring mode. The transition m/z 369.0 → 119.0, m/z 312.9 → 117.9, m/z 329.0 → 196.0, and m/z 307.1 → 161.3 were used for monitoring PX, V...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Aromatic aldehydes as selective fluorogenic derivatizing agents for α‐dicarbonyl compounds. Application to HPLC analysis of some advanced glycation end products and oxidative stress biomarkers in human serum
Publication date: 5 September 2018Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 158Author(s): Mahmoud H. El-Maghrabey, Taro Nakatani, Naoya Kishikawa, Naotaka KurodaAbstractα-Dicarbonyl compounds (α-DCs) are very clinically important as they are considered as advanced glycation end products (AGEs) precursors and biomarkers for many chronic diseases such as diabetes and vascular diseases, in addition to their major role in progression of complications of such diseases. Aromatic aldehydes and ammonium acetate were productively used as a one-pot co-reagents for fluorogenic derivatization of &al...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Simultaneous quantitation of folates, flavins and B6 metabolites in human plasma by LC–MS/MS assay: Applications in colorectal cancer
Publication date: 5 September 2018Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 158Author(s): Isaac Asante, Hua Pei, Eugene Zhou, Siyu Liu, Darryl Chui, EunJeong Yoo, Stan G. LouieAbstractAn analytical method using electrospray ionization and high- performance liquid chromatography/tandem mass spectrometry (LC/ESI–MS/MS) was developed to quantify the vitamin B metabolites found in the folate one-carbon metabolism, using 50 μL of human plasma. Analytes in plasma were extracted using protein precipitation after being stabilized in 1% ascorbic acid. The analytes were separated using a Kinetex 2....
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Validated LC–MS/MS method for the determination of amlodipine enantiomers in rat plasma and its application to a stereoselective pharmacokinetic study
Publication date: 5 September 2018Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 158Author(s): Lijuan Wang, Wenxia Liu, Zunjian Zhang, Yuan TianAbstractA rapid and sensitive method was established to determine amlodipine enantiomers using liquid chromatography-tandem mass spectrometry (LC–MS/MS). Stereoselective separation was performed on CHIRALCEL OZ-RH column (150 mm × 4.6 mm i.d., 5 μm) with acetonitrile-water (10 mM ammonium acetate, 0.5% ammonia solution) (95:5, v/v) at a flow rate of 0.5 mL/min. The substances were detected by mass spectrometer equipped with an electro...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Metabolomics coupled with system pharmacology reveal the protective effect of total flavonoids of Astragali Radix against adriamycin-induced rat nephropathy model
In this study, a NMR based metabolomic approach coupled with biochemical assay and histopathological inspection had been employed to study the protective effect of total flavonoids (TFA) in AR against adriamycin-induced nephropathy using rats model. Multivariate analysis revealed that 11 of perturbed metabolites could be reversed by TFA, and the MetaboAnalyst analysis revealed that the anti-nephrotic syndrome effect of TFA was probably related with regulation of alanine, aspartate and glutamate metabolism, citrate cycle, pyruvate metabolism, cysteine and methionine metabolism and glyoxylate and dicarboxylate metabolism. Th...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Development and validation of an ICP-MS method for quantification of total carbon and platinum in cell samples and comparison of open-vessel and microwave-assisted acid digestion methods
Publication date: 5 September 2018Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 158Author(s): Mie Riisom, Bente Gammelgaard, Ian H. Lambert, Stefan StürupAbstractCisplatin is a widely used chemotherapeutic drug. Due to severe side effects and intrinsic or acquired resistance, there is a great interest in developing new platinum-based anticancer agents and a need for robust and validated analytical methods for determination of platinum accumulation in biological samples. A validated ICP-MS method for quantification of total carbon and platinum in cell samples is presented, applicable for cellular dr...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Metabolome based classification of artichoke leaf: A prospect for phyto-equivalency of its different leaf origins and commercial preparations
Publication date: 5 September 2018Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 158Author(s): Mohamed A. Farag, Mahmoud F. Elsebai, Amira R. KhattabAbstractThe growing interest in assuring phytomedicines efficacy and moreover the increase in requirements for its safety drive the development of analytical methods for its quality control assurance. Herein, we present a nuclear magnetic resonance (NMR) fingerprinting approach of artichoke leaf material from different origins and in its commercial preparations. Under optimized conditions, we were able to simultaneously identify 23 metabolites including suga...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Monitoring of tobramycin in human plasma via mixed matrix membrane extraction prior to capillary electrophoresis with contactless conductivity detection
Publication date: 5 September 2018Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 158Author(s): Nurul Hazirah Mukhtar, Nor Akma Mamat, Hong Heng SeeAbstractA sample pre-treatment method based on a dynamic mixed matrix membrane tip extraction followed by capillary electrophoresis with contactless conductivity detection (CE-C4D) was evaluated for the determination of tobramycin in human plasma. The extraction tip device consisted of a cellulose triacetate membrane tip wall immobilised with 15% (w/w) of hydrophilic lipophilic balance (HLB) nanoparticles as adsorbent. The extraction was performed dynamically ...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Doping control analysis of 121 prohibited substances in equine hair by liquid chromatography–tandem mass spectrometry
Publication date: 5 September 2018Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 158Author(s): J.K.Y. Wong, T.L.S. Choi, K.Y. Kwok, E.N.Y. Lei, T.S.M. WanAbstractEquine hair is becoming an increasingly popular biological matrix for doping control of horse sports; one of the reasons for this is the significantly longer detection window hair can offer. Hair analysis opens up the opportunity for longitudinal monitoring of drug exposure which would otherwise not be possible with the more traditional and common biological matrices, such as urine and blood. As such, there is a need for more multi-target screen...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Simultaneous determination of plasma methotrexate and 7-hydroxy methotrexate by UHPLC–MS/MS in patients receiving high-dose methotrexate therapy
Publication date: 5 September 2018Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 158Author(s): Shenghui Mei, Xiaoxu Shi, Yaming Du, Yong Cui, Chun Zeng, Xiaohui Ren, Kefu Yu, Zhigang Zhao, Song LinAbstractThe plasma concentrations of methotrexate (MTX) and its major metabolite 7-hydroxy methotrexate (7-OH-MTX) are highly correlated with the toxicities in patients with high-dose MTX therapy. Routine monitoring of MTX and 7-OH-MTX plasma levels is useful for dose adjustment of rescue drugs and toxicity prevention. A UHPLC-MS/MS method for simultaneous determination of plasma MTX and 7-OH-MTX was developed,...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Gender-related differences in pharmacokinetics, tissue distribution, and excretion of 20(R)-25-methoxyl-dammarane-3β,12β,20-triol and its metabolite in rats and anti-ovarian cancer evaluation
Publication date: 5 September 2018Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 158Author(s): Meng Ding, Yumeng Zhang, Xude Wang, Yuqing ZhaoAbstractOur previous study suggests a well-defined pharmacological activity of 20(R)-25-methoxyl-dammarane-3β,12β,20-triol (AD-1) and its metabolite 20(R)-dammarane-3β,12β,20,25-tetrol (AD-2). The current study aims to investigate the gender-related differences in pharmacokinetics, tissue distribution, and excretion of AD-1 and its metabolite in both male and female rats. A sensitive and rapid ultra-performance liquid chromatography tandem mass ...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Optimization of LC method for the quantification of doxorubicin in plasma and urine samples in view of pharmacokinetic, biomedical and drug monitoring therapy studies
Publication date: 5 September 2018Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 158Author(s): Olga Maliszewska, Alina Plenis, Ilona Olędzka, Piotr Kowalski, Natalia Miękus, Ewa Bień, Małgorzata Anna Krawczyk, Elżbieta Adamkiewicz-Drożynska, Tomasz BączekAbstractA simple, rapid, reliable and sensitive method based on liquid chromatography with fluorescence detection (LC-FL) for the quantification of doxorubicin (DOX) in human plasma and urine samples was developed. The assay was carried out after the solid-phase extraction procedure (SPE) with hydrophilic-lipophilic balance (HLB) cartridges, and w...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

A rapid and sensitive LC–MS/MS method for quantitative analysis of cardiolipin (18:2)4 in human leukocytes and mouse skeletal muscles
Publication date: 5 September 2018Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 158Author(s): Gang Xu, Xiao Liu, Yachun Shu, Jagan A. Pillai, Yan XuAbstractA rapid and sensitive LC–MS/MS method has been developed for quantitative analysis of cardiolipin (18:2)4 or CL (18:2)4 in human leukocytes and mouse mitochondria. The structural analog CL (14:0)4 was used as the internal standard. Both CL (18:2)4 and the IS were extracted using a modified Folch method, and separated on a Waters XBridge® BEH C18 XP column using a mobile phase of 0.1% ammonium hydroxide in acetonitrile/water (90:10, v/v) pum...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Development of a simple, rapid and high-throughput fluorescence polarization immunoassay for glycocholic acid in human urine
Publication date: 5 September 2018Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 158Author(s): Qiyi He, Xiping Cui, Ding Shen, Yingshan Chen, Zhengyun Jiang, Rui Lv, Sergei A. Eremin, Suqing ZhaoAbstractIn this paper, a simple, rapid and high-throughput fluorescence polarization immunoassay (FPIA) based on polyclonal antibodies (PAb) is described for the determination of glycocholic acid (GCA) in human urine. Three fluorescein-labeled GCA (tracers) with different structures and spacer bridges were synthesized and purified by thin-layer chromatography (TLC). The structure effect of tracers on the assay wa...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Plasma and urine metabolite profiling reveals the protective effect of Clinacanthus nutans in an ovalbumin-induced anaphylaxis model: 1H-NMR metabolomics approach
Publication date: 5 September 2018Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 158Author(s): Leng Wei Khoo, Siew Foong Audrey Kow, M. Maulidiani, Ming Tatt Lee, Chin Ping Tan, Khozirah Shaari, Chau Ling Tham, Faridah AbasAbstractThe present study sought to identify the key biomarkers and pathways involved in the induction of allergic sensitization to ovalbumin and to elucidate the potential anti-anaphylaxis property of Clinacanthus nutans (Burm. f.) Lindau water leaf extract, a Southeast Asia herb in an in vivo ovalbumin-induced active systemic anaphylaxis model evaluated by 1H-NMR metabolomics. The re...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Mechanism of antidiabetic and synergistic effects of ginseng polysaccharide and ginsenoside Rb1 on diabetic rat model
Publication date: 5 September 2018Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 158Author(s): Jing Li, Ruigang Li, Na Li, Fei Zheng, Yulin Dai, Yan Ge, Hao Yue, Shanshan YuAbstractGinseng polysaccharides (GP) have been reported to modulate gut microbiota, and ginsenoside Rb1 is known to display significant hypoglycemic activity. However, the synergistic effect of Rb1 and GP when applied to diabetic treatment remains largely unknown. Male rats were divided into ten groups: blank group (B-Group), model group (D-Group), Rb1 group (Rb1-Group), CK group (CK-Group), GP groups and GP + Rb1 groups in do...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

pH-Dependent stability of azithromycin in aqueous solution and structure identification of two new degradation products
Publication date: 5 September 2018Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 158Author(s): Maria Grazia Saita, Danilo Aleo, Barbara Melilli, Sergio Mangiafico, Melina Cro, Claudia Sanfilippo, Angela PattiAbstractThe degradation profile of azithromycin in buffered solutions was investigated using HPLC and found to be pH dependent in the range of 6.0–7.2. Desosaminylazitromycin, derived from hydrolytic loss of cladinose of the parent molecule, was the major degradation product at pH 6.0 but its amount progressively decreased moving toward pH 7.2. Two additional unreported degradation products wer...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Simultaneous quantitation of two direct acting hepatitis C antivirals (sofosbuvir and daclatasvir) by an HPLC-UV method designated for their pharmacokinetic study in rabbits
Publication date: 5 September 2018Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 158Author(s): Noha N. Atia, Salwa R. El-Shaboury, Samia M. El-Gizawy, Mohammad Nabil Abo-ZeidAbstractSofosbuvir (SOF) and daclatasvir (DCS) are novel, recently developed direct acting antiviral agents characterized by potent anti-hepatitis C virus action. A fast and efficient HPLC-UV method was developed, validated and applied for simultaneous determination of SOF and DCS in pharmaceutical formulations and biological fluids based on coupling liquid-liquid extraction with ultrasound and dual wavelength detection at λma...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Development and validation of a liquid chromatography-tandem mass spectrometry assay for the quantification of lurbinectedin in human plasma and urine
This article describes the development and validation of a liquid chromatography-tandem mass spectrometry (LC–MS/MS) assay to quantify lurbinectedin in human plasma and urine. Plasma samples were pre-treated with 1 M aqueous ammonia after which they were brought onto supported liquid extraction (SLE) columns. Lurbinectedin was eluted from the columns using tert-butyl methyl ether (TBME). Urine was first diluted in plasma and lurbinectedin was extracted from this matrix by liquid-liquid extraction using TBME. Samples were measured by LC–MS/MS in the positive electron ion spray mode. The method was linear over ...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Liquid chromatography-tandem mass spectrometric assay for therapeutic drug monitoring of the EGFR inhibitors afatinib, erlotinib and osimertinib, the ALK inhibitor crizotinib and the VEGFR inhibitor nintedanib in human plasma from non-small cell lung cancer patients
Publication date: 5 September 2018Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 158Author(s): Rafael Reis, Laurence Labat, Marie Allard, Pascaline Boudou-Rouquette, Jeanne Chapron, Audrey Bellesoeur, Audrey Thomas-Schoemann, Jennifer Arrondeau, Frédérique Giraud, Jérôme Alexandre, Michel Vidal, François Goldwasser, Benoît BlanchetAbstractA new method for the quantitative analysis by liquid chromatography-tandem mass spectrometry (LC–MS/MS) of five tyrosine kinase inhibitors (afatinib, crizotinib, osimertinib, erlotinib and nintedanib) used in the treatment o...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Real-time quantitative PCR detection of circulating tumor cells using tag DNA mediated signal amplification strategy
Publication date: 5 September 2018Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 158Author(s): Ting mei, Xuewen Lu, Ning Sun, Xiaomei Li, Jitao Chen, Min Liang, Xinke Zhou, Zhiyuan FangAbstractThe level of circulating tumor cell (CTCs) is a reliable marker for tumor burden and malignant progression. Quantification of CTCs remains technically challenging due to the rarity of these cells in peripheral blood. In the present study, we established a real-time quantitative PCR (Q-PCR) based method for sensitive detection of CTCs without DNA extraction. Blood sample was first turned to erythrocyte lyses and the...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Identification of a new tadalafil analogue, N-3-hydroxypropylnortadalafil, in a supplement product
Publication date: 5 September 2018Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 158Author(s): Hui-Chun Lee, Yun-Lian Lin, Yen-Chun Huang, Chia-Fen Tsai, Der-Yuan WangAbstractA novel tadalafil analogue, which exhibits similarity to 2-hydroxypropylnortadalafil, was found in dietary supplements using adulterants screening and isolated using column chromatography. By using extensive 1D- and 2D-NMR and MS spectral analyses, the structure was determined as 6-(1,3-Benzodioxol-5-yl)-2,3,6,7,12,12a-hexahydro-2-(3-hydroxypropyl)pyrazino(1′,2′:1,6)pyrido(3,4-b)indole-1,4-dione, and the analogue was nam...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Natural deep eutectic solvents as eco-friendly and sustainable dilution medium for the determination of residual organic solvents in pharmaceuticals with static headspace-gas chromatography
Publication date: 5 September 2018Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 158Author(s): Meilian Wang, Sheng Fang, Xianrui LiangAbstractReported here is a simple and rapid static headspace gas chromatography (SHS-GC) method for the determination of trace solvents including ethanol, isopropanol, n-butanol, 1,4-dioxane, tetrahydrofuran, acetonitrile, methanol and acetone which commonly used in drug production process. Natural deep eutectic solvents (NADESs) are firstly used as the matrix medium for this method, which provided high sensitivity for residual solvents detection. With the optimized method...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

RNA-seq based transcriptome analysis of the protective effect of compound longmaining decoction on acute myocardial infarction
This study demonstrated that CLMN decoction might regulate the expressions of Ccl6, Ccr5, Itgam, Ncf1 and Mmp9, inhibit the chemokine signaling pathway and leukocyte transendothelial migration to play a protective effect on AMI.Graphical abstract (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Analysis of monofluoroacetic acid in human plasma by UFLC–MS/MS and its application in patients with sodium monofluoroacetate or monofluoroacetamide poisoning
Publication date: 5 September 2018Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 158Author(s): Liang Liu, Shuang Hu, Suodi Zhai, Xin HaiAbstractMonofluoroacetic acid (FAcOH), was once widely used in baits as a rodenticide in agriculture. For intentional and unintentional misuses, the incidence of FAcOH poisoning has increased in recent years. Organic fluorine rodenticides such as sodium monofluoroacetate and monofluoroacetamide, the analogs of FAcOH, can easily be converted to FAcOH in vivo and cause injury. It is urgent to establish a simple and sensitive analytical method of FAcOH in human plasma and a...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research