Acute In Vivo Testing of a Polymer Cuff Electrode with Integrated Microfluidic Channels for Stimulation, Recording, and Drug Delivery on Rat Sciatic Nerve
ConclusionThe feasibility of using a cuff having integrated microelectrodes and microfluidics to stimulate, record, and deliver drug to focally lyse away the epineurium layer was demonstrated in acute experiments on rat sciatic nerve. (Source: Journal of Neuroscience Methods)
Source: Journal of Neuroscience Methods - February 15, 2020 Category: Neuroscience Source Type: research

Novel Ablation Methods for Treatment of Gliomas
Publication date: Available online 14 February 2020Source: Journal of Neuroscience MethodsAuthor(s): Brittanie Partridge, John H. Rossmeisl, Alexandra M. Kaloss, Erwin Kristobal Gudenschwager Basso, Michelle H. TheusAbstractPrimary brain tumors are among the deadliest cancers that remain highly incurable. A need exists for new approaches to tumor therapy that can circumvent the blood brain barrier (BBB), target highly resistant tumors and cancer stem-like cells (CSCs) as well create an anti-cancer immunomodulatory environment. Successful treatments may also require a combinatory approach utilizing surgery, chemotherapy, ra...
Source: Journal of Neuroscience Methods - February 15, 2020 Category: Neuroscience Source Type: research

ASCL1- and DLX2-induced GABAergic neurons from hiPSC-derived NPCs
ConclusionsThis methodology, relying upon an easily cultured starting population of hiPSC-NPCs, makes possible the generation of large-scale defined co-cultures of induced glutamatergic and GABAergic neurons for hiPSC-based disease models and precision drug screening. (Source: Journal of Neuroscience Methods)
Source: Journal of Neuroscience Methods - February 15, 2020 Category: Neuroscience Source Type: research

3D in vitro test system for vestibular schwannoma
Publication date: Available online 12 February 2020Source: Journal of Neuroscience MethodsAuthor(s): Maria Breun, Donato Daniel Martellotta, Anna Leberle, Sarah Nietzer, Florentin Baur, Ralf-Ingo Ernestus, Cordula Matthies, Mario Löhr, Carsten Hagemann (Source: Journal of Neuroscience Methods)
Source: Journal of Neuroscience Methods - February 13, 2020 Category: Neuroscience Source Type: research

Susceptibility Artifact Correction for Sub-millimeter fMRI using Inverse Phase Encoding Registration and T1 Weighted Regularization
ConclusionsThe proposed correction method used the T1w image as a reference in the inverse-PE registration. Results show its promising performance. Our proposed method is timely, as sub-millimeter fMRI has become increasingly popular. (Source: Journal of Neuroscience Methods)
Source: Journal of Neuroscience Methods - February 13, 2020 Category: Neuroscience Source Type: research

A methodological investigation of a flexible surface MRI coil to obtain functional signals from the human olfactory bulb
Publication date: 1 April 2020Source: Journal of Neuroscience Methods, Volume 335Author(s): A. Fournel, E. Iannilli, C. Ferdenzi, A. Werner, T. Hummel, M. BensafiAbstractBackgroundMammalian olfaction begins with transduction in olfactory receptors, continues with extensive processing in the olfactory bulb, and culminates in cortical representation. Most rodent studies on the functional neuroanatomy of olfaction have concentrated on the olfactory bulb, yet whether this structure is tuned only to basic chemical features of odorants or also to higher-order perceptual features is unclear.New methodWhereas studies of the human ...
Source: Journal of Neuroscience Methods - February 13, 2020 Category: Neuroscience Source Type: research

An Enhanced Frequency Dependent Subtraction Algorithm for Removal of Cardiac Interference from fMEG by Using Minimum Norm Projection Operator
ConclusionsSUBTRwMNPO is successful in removing cardiac interference regardless of the orientation of fMCG and fMEG signal spaces. It can also be used to remove cardiac interference when there is no prior knowledge of fetal head location. (Source: Journal of Neuroscience Methods)
Source: Journal of Neuroscience Methods - February 12, 2020 Category: Neuroscience Source Type: research

An EEG-fNIRS Hybridization Technique in the Four-Class Classification of Alzheimer’s Disease
ConclusionsThese findings suggest that hybrid EEG-fNIRS systems are a promising tool that may enhance the AD diagnosis and assessment process. (Source: Journal of Neuroscience Methods)
Source: Journal of Neuroscience Methods - February 10, 2020 Category: Neuroscience Source Type: research

Viral approaches to study the mammalian brain: lineage tracing, circuit dissection and therapeutic applications
Publication date: Available online 8 February 2020Source: Journal of Neuroscience MethodsAuthor(s): Wei Cong, Yun Shi, Yanqing Qi, Jinyun Wu, Ling Gong, Miao HeAbstractViral vectors are widely used to study the development, function and pathology of neural circuits in the mammalian brain. Their flexible payloads with customizable choices of tool genes allow versatile applications ranging from lineage tracing, circuit mapping and functional interrogation, to therapeutic applications. Different applications have distinct technological requirements, therefore, often utilize different types of virus. This review introduces the...
Source: Journal of Neuroscience Methods - February 10, 2020 Category: Neuroscience Source Type: research

Three-dimensional mapping of tyrosine hydroxylase in the transparent brain and adrenal of prenatal and pre-weaning mice: Comprehensive methodological flowchart and quantitative aspects of 3D mapping
ConclusionThe procedures described and the extensive 3D mapping of TH immunoreactivity at early embryonic and postnatal stages provide a comprehensive view of the onset and development of the catecholaminergic system in the mouse brain and sympathoadrenal nervous system. (Source: Journal of Neuroscience Methods)
Source: Journal of Neuroscience Methods - February 6, 2020 Category: Neuroscience Source Type: research

Resolution-based Spectral Clustering for Brain Parcellation using Functional MRI
Conclusions: Clustering of resolution provides a principled and robust approach to brain parcellation which improves on the current baseline. (Source: Journal of Neuroscience Methods)
Source: Journal of Neuroscience Methods - February 6, 2020 Category: Neuroscience Source Type: research

A Methodological Investigation of a Dental MRI Coil to Obtain Functional Signals from the Human Olfactory Bulb
ConclusionsThe present preliminary technical achievement renders possible to design novel human odor fMRI studies by considering the olfactory system from the olfactory bulb to associative areas. (Source: Journal of Neuroscience Methods)
Source: Journal of Neuroscience Methods - February 6, 2020 Category: Neuroscience Source Type: research

High-throughput screening of human induced pluripotent stem cell-derived brain organoids
ConclusionsSFEBs can be used in combination with HTS to compensate for experimental variability common in 3D cultures, while significantly decreasing processing speed, making this an efficient starting point for phenotypic drug screening.Graphical Abstract (Source: Journal of Neuroscience Methods)
Source: Journal of Neuroscience Methods - February 6, 2020 Category: Neuroscience Source Type: research

Functional Clustering of Whole Brain White Matter Fibers
ConclusionThe proposed concept and framework can be used to identify functional pathways and their structural features under specific function loading. (Source: Journal of Neuroscience Methods)
Source: Journal of Neuroscience Methods - February 6, 2020 Category: Neuroscience Source Type: research

Invited Review: Utilizing Peripheral Nerve Regenerative Elements to Repair Damage in the CNS
Publication date: Available online 3 February 2020Source: Journal of Neuroscience MethodsAuthor(s): Nader El Seblani, Andrew Welleford, Jorge E. Quintero, Craig van Horne, Greg A. GerhardtAbstractAn ongoing question in neuroscience is how the peripheral nervous system can repair itself following an injury or insult whereas the central nervous system has a profoundly limited ability for repair. The recent and rapid advancement of our understanding of the gene expression and corresponding biochemical profiles of Schwann cells, within the distal segments of injured peripheral nerves, has helped elucidate the potential mechani...
Source: Journal of Neuroscience Methods - February 4, 2020 Category: Neuroscience Source Type: research

LFP-Net: A Deep Learning Framework to Recognize Human Behavioral Activities using Brain STN-LFP Signals
ConclusionsCNNs show a high performance in decoding the brain neural response, which is crucial in designing the automatic brain-computer interfaces and closed-loop systems. (Source: Journal of Neuroscience Methods)
Source: Journal of Neuroscience Methods - February 4, 2020 Category: Neuroscience Source Type: research

The gamma model analysis (GMA): Introducing a novel scoring method for the shape of components of the event-related potential
ConclusionThis approach, therefore, provides a novel toolset to better understand the exact relationship between ERP components, behaviour and cognition. (Source: Journal of Neuroscience Methods)
Source: Journal of Neuroscience Methods - February 3, 2020 Category: Neuroscience Source Type: research

A novel-odour exploration test for measuring anxiety in adult and larval zebrafish
ConclusionsThe NOEt is a simple, rapid and low-cost test to study anxiety in zebrafish using the spontaneous exploration of novel olfactory cues. (Source: Journal of Neuroscience Methods)
Source: Journal of Neuroscience Methods - February 3, 2020 Category: Neuroscience Source Type: research

Optimization of production and transgene expression of a retrogradely transported pseudotyped lentiviral vector
ConclusionWe have developed a method that allows for specific analysis of neuronal subpopulations in the brain either by microscopy or by biochemical methods e.g. immunoprecipitation. This method is simple to use and can be combined with transgenic animals for studying disease models. (Source: Journal of Neuroscience Methods)
Source: Journal of Neuroscience Methods - February 3, 2020 Category: Neuroscience Source Type: research

External noise removed from magnetoencephalographic signal using Independent Component Analyses of reference channels
ConclusionsThe proposed method complements and extends traditional reference based noise correction with little extra computational cost and low chances of false positives. Any MEG system with reference channels could profit from its use, particularly in labs with intermittent noise sources. (Source: Journal of Neuroscience Methods)
Source: Journal of Neuroscience Methods - February 3, 2020 Category: Neuroscience Source Type: research

Observational learning of a shifting goal location in rats: Impact of distance, observed performance, familiarity, and delay
ConclusionsThe current paradigm allows for repeated examinations of observational learning and provides an alternative method for neurobiological studies of social learning. (Source: Journal of Neuroscience Methods)
Source: Journal of Neuroscience Methods - February 1, 2020 Category: Neuroscience Source Type: research

Current approaches to modeling the virtual reality in rodents for the assessment of brain plasticity and behavior
Publication date: Available online 30 January 2020Source: Journal of Neuroscience MethodsAuthor(s): Olga L. Lopatina, Andrey V. Morgun, Yana V. Gorina, Vladimir V. Salmin, Alla B. SalminaAbstractVirtual reality(VR) and augmented reality (AR) have become valuable tools to study brains and behaviors resulting in development of new methods of diagnostics and treatment. Neurodegenerаtion is one of the best examples demonstrating efficacy of VR/АR technologies in modern neurology. Development of novel VR systems for rodents and combination of VR tools with up-to-date imaging techniques (i.e. MRI, imaging of neural networks et...
Source: Journal of Neuroscience Methods - February 1, 2020 Category: Neuroscience Source Type: research

Controllable Forces for Reproducible Chronic Constriction Injury Mimicking Compressive Neuropathy in Rat Sciatic Nerve
ConclusionThe functional influences of different compressive forces recapitulate the diverse clinical symptoms involved in clinical compressive neuropathy. This controllable and reproducible model of compressive neuropathy revealed the underlying molecular mechanisms of neural degeneration and inflammation. It will lead to the future development of translational therapeutics for neuropathic pain and nerve regeneration. (Source: Journal of Neuroscience Methods)
Source: Journal of Neuroscience Methods - January 31, 2020 Category: Neuroscience Source Type: research

Discriminating VCID subgroups: A diffusion MRI multi-model fusion approach
ConclusionOverall our results highlight the benefits of multi-model fusion approach, providing improved sensitivity in discriminating VCID subgroups. (Source: Journal of Neuroscience Methods)
Source: Journal of Neuroscience Methods - January 29, 2020 Category: Neuroscience Source Type: research

DeepFMRI: End-to-end deep learning for functional connectivity and classification of ADHD using fMRI
Conclusions The results suggest that the proposed end-to-end deep learning architecture achieves better performance as compared to the other state-of-the-art methods. The findings suggest that the frontal lobe contains the most discriminative power towards the classification of ADHD. (Source: Journal of Neuroscience Methods)
Source: Journal of Neuroscience Methods - January 29, 2020 Category: Neuroscience Source Type: research

Comparison of feature selection methods based on discrimination and reliability for fMRI decoding analysis
ConclusionThe results from this study provide empirical guides for feature selection for the prediction of individual brain states. (Source: Journal of Neuroscience Methods)
Source: Journal of Neuroscience Methods - January 29, 2020 Category: Neuroscience Source Type: research

Lessons, insights and newly developed tools emerging from behavioral phenotyping core facilities
Publication date: Available online 24 January 2020Source: Journal of Neuroscience MethodsAuthor(s): Lior Bikovski, Lianne Robinson, Asa Konradsson-Geuken, Klas Kullander, Thomas Viereckel, Svante Winberg, Erika Roman, Michael TsooryAbstractScientific investigations, in general, and research in neuroscience, in particular, are becoming ever more complex and require the integration of different techniques. Behavioral assays, which are among the most frequently used methodologies in neuroscience, nowadays rely on advanced, sophisticated technologies that require proficient application. Therefore, behavioral core facilities ar...
Source: Journal of Neuroscience Methods - January 25, 2020 Category: Neuroscience Source Type: research

Assessment of anesthesia on physiological stability and bold signal reliability during visual or acoustic stimulation in the cat
ConclusionsAlfaxalone is observed to be more physiologically stable, evoking a more consistent BOLD signal across animals than the co-administration of ketamine and isoflurane. Thus, an alfaxalone-based protocol may represent a better approach for neuroimaging in animal models requiring anesthesia.Graphical abstract (Source: Journal of Neuroscience Methods)
Source: Journal of Neuroscience Methods - January 25, 2020 Category: Neuroscience Source Type: research

Editorial Board
Publication date: 1 March 2020Source: Journal of Neuroscience Methods, Volume 333Author(s): (Source: Journal of Neuroscience Methods)
Source: Journal of Neuroscience Methods - January 25, 2020 Category: Neuroscience Source Type: research

Cuff and sieve electrode (CASE): the combination of neural electrodes for bi-directional peripheral nerve interfacing
Publication date: Available online 22 January 2020Source: Journal of Neuroscience MethodsAuthor(s): Hyungsoo Kim, Aaron M. Dingle, Jared P. Ness, Dong-Hyun Baek, Jihye Bong, In-Kyu Lee, Nikita O. Shulzhenko, Weifeng Zeng, Jacqueline S. Israel, Jane A. Pisaniello, Augusto X.T. Millevolte, Dong-Wook Park, Aaron J. Suminski, Yei Hwan Jung, Justin C. Williams, Samuel O. Poore, Zhenqiang MaAbstractBackgroundA number of peripheral nerve interfaces for nerve stimulation and recording exist for the purpose of controlling neural prostheses, each with a set of advantages and disadvantages. The ultimate goal of neural prostheses is a...
Source: Journal of Neuroscience Methods - January 23, 2020 Category: Neuroscience Source Type: research

Transcardial Perfusion is not Required to Accurately Measure Cytokines within the Brain
ConclusionsBrain cytokine concentrations are unaffected by perfusing with saline prior to tissue collection; this holds true regardless of immune status (homeostatic or immune challenged), suggesting that this time-consuming step may be unnecessary. (Source: Journal of Neuroscience Methods)
Source: Journal of Neuroscience Methods - January 23, 2020 Category: Neuroscience Source Type: research

Weighted average of shared trajectory: a new estimator for dynamic functional connectivity efficiently estimates both rapid and slow changes over time
ConclusionsAs large window sizes are unable to detect short states, using shorter window size is desirable if the estimator is robust enough. We provide evidence that WAST requires fewer samples (compared to SWPC) to reach a robust estimation. As a result, we were able to identify rapidly varying dFNC patterns undetected by SWPC while still being able to robustly estimate slower dFNC patterns. (Source: Journal of Neuroscience Methods)
Source: Journal of Neuroscience Methods - January 22, 2020 Category: Neuroscience Source Type: research

Odor-induced emotion recognition based on average frequency band division of EEG signals
ConclusionsThe SVM plus AFBD method represents a useful contribution to olfactory-induced emotion recognition. Classification of the five-emotion categories was generally inferior to the classification of the two-emotion categories, suggesting that the recognition performance decreased as the number of emotions in the category increased. (Source: Journal of Neuroscience Methods)
Source: Journal of Neuroscience Methods - January 22, 2020 Category: Neuroscience Source Type: research

A framework for quality control of corpus callosum segmentation in large-scale studies
Conclusions: The shape descriptor is robust and versatile, describing the segmentation at different resolutions. The selection of classifiers and the disagreement measure lead to an ensemble composed of high-quality and heterogeneous classifiers, ensuring an optimal trade-off between the ensemble size and high AUC.Graphical abstract (Source: Journal of Neuroscience Methods)
Source: Journal of Neuroscience Methods - January 22, 2020 Category: Neuroscience Source Type: research

Imaging neuronal activity in the central and peripheral nervous systems using new Thy1.2-GCaMP6 transgenic mouse lines
ConclusionsThese GCaMP6 transgenic lines thus provide useful tools for functional analysis of neuronal circuits in both central and peripheral nervous systems. (Source: Journal of Neuroscience Methods)
Source: Journal of Neuroscience Methods - January 22, 2020 Category: Neuroscience Source Type: research

Automated quantification of head-twitch response in mice via ear tag reporter coupled with biphasic detection
ConclusionsHigh-throughput identification of HTR utilizing magnetic ear tagging and biphasic detection delivers satisfactory detection and discrimination power employing less invasive procedures. (Source: Journal of Neuroscience Methods)
Source: Journal of Neuroscience Methods - January 17, 2020 Category: Neuroscience Source Type: research

Reconfigurable 3D-Printed headplates for reproducible and rapid implantation of EEG, EMG and depth electrodes in mice
Conclusions3D printing and friction-fit pre-assembly of mouse headplates offers a rapid, easily reconfigurable, consistent, and cost-effective way to implant larger numbers of mice in a highly reproducible way, reducing surgical time and mitigating experimental error. (Source: Journal of Neuroscience Methods)
Source: Journal of Neuroscience Methods - January 16, 2020 Category: Neuroscience Source Type: research

Comparing SPM12 and CAT12 segmentation pipelines: a brain tissue volume-based age and Alzheimer’s disease study
ConclusionsOur findings indicate that other studies should take these pipeline effects on age and AD diagnosis, into account, for improved comparability in previous literature. Additionally, we encourage future studies to use CAT12 as this is a more advanced and computationally efficient brain segmentation tool. (Source: Journal of Neuroscience Methods)
Source: Journal of Neuroscience Methods - January 16, 2020 Category: Neuroscience Source Type: research

Clearing Techniques for Visualizing the Nervous System in Development, Injury, and Disease
Publication date: Available online 13 January 2020Source: Journal of Neuroscience MethodsAuthor(s): Demisha D.L. Porter, Paul D. MortonAbstractModern clearing techniques enable high resolution visualization and 3D reconstruction of cell populations and their structural details throughout large biological samples, including intact organs and even entire organisms. In the past decade, these methods have become more tractable and are now being utilized to provide unforeseen insights into the complexities of the nervous system. While several iterations of optical clearing techniques have been developed, some are more suitable ...
Source: Journal of Neuroscience Methods - January 14, 2020 Category: Neuroscience Source Type: research

A modified Barnes maze for an accurate assessment of spatial learning in mice
ConclusionsSpatial learning in the MBM is a target-location sensitive process, providing flexibility in task difficulty. Along with overcoming biases towards non-spatial strategies, the MBM represents an improvement over the well-validated BM. (Source: Journal of Neuroscience Methods)
Source: Journal of Neuroscience Methods - January 10, 2020 Category: Neuroscience Source Type: research

Comparing connectivity metrics in cortico-cortical evoked potentials using synthetic cortical response patterns
ConclusionsOur novel method showed that RMS is a robust and sensitive measure, validating much of the findings of the SEEG-CCEP literature to date. Autoregressive coefficients may also be a useful metric to investigate epileptic networks. (Source: Journal of Neuroscience Methods)
Source: Journal of Neuroscience Methods - January 10, 2020 Category: Neuroscience Source Type: research

Multiplex LA-ICP-MS bio-imaging of brain tissue of a parkinsonian mouse model stained with metal-coded affinity-tagged antibodies and coated with indium-spiked commercial inks as internal standards
ConclusionsInternal standardisation using indium-doped inks is an effective method to overcome day-to-day variations and instrumental drifts. The new approach results in an increased signal-to-noise ratio and only under these conditions small but significant changes were detected, as seen for tyrosine hydroxylase. (Source: Journal of Neuroscience Methods)
Source: Journal of Neuroscience Methods - January 10, 2020 Category: Neuroscience Source Type: research

Identification of the Human Postural Control System through Stochastic Trajectory Optimization
ConclusionsStochastic identification can be used to avoid unstable controllers in human postural control identification. (Source: Journal of Neuroscience Methods)
Source: Journal of Neuroscience Methods - January 10, 2020 Category: Neuroscience Source Type: research

Automatic detection and quantification of hand movements toward development of an objective assessment of tremor and bradykinesia in Parkinson's disease
ConclusionsThe proposed study provides an objective assessment of tremor and bradykinesia in Parkinson's disease. Accordingly, this may help movement disorder clinicians to detect, diagnose and monitor treatment efficacy in Parkinson's disease. (Source: Journal of Neuroscience Methods)
Source: Journal of Neuroscience Methods - January 8, 2020 Category: Neuroscience Source Type: research

Editorial Board
Publication date: 15 February 2020Source: Journal of Neuroscience Methods, Volume 332Author(s): (Source: Journal of Neuroscience Methods)
Source: Journal of Neuroscience Methods - January 8, 2020 Category: Neuroscience Source Type: research

Automated Segmentation of Acute Stroke Lesions Using a Data-driven Anomaly Detection on Diffusion Weighted MRI
ConclusionsThe current method is straightforward, fast, and shows good agreement with the lesions identified by human experts. (Source: Journal of Neuroscience Methods)
Source: Journal of Neuroscience Methods - January 3, 2020 Category: Neuroscience Source Type: research

In vivo validation of a new portable stimulator for chronic deep brain stimulation in freely moving rats
ConclusionWe successfully developed and validated a new portable and programmable stimulator for freely moving rats that delivers a large range of stimulation parameters using bilateral biphasic current-control and charge balancing to maximize tissue safety. This device can be used to test deep brain stimulation in different animal models of human brain diseases. (Source: Journal of Neuroscience Methods)
Source: Journal of Neuroscience Methods - January 1, 2020 Category: Neuroscience Source Type: research

A Simple DMSO-Based Method for Cryopreservation of Primary Hippocampal and Cortical Neurons
ConclusionsIntroduction of the cryopreserved neurons as a standard laboratory practice has the potential to increase the robustness and reproducibility of findings between laboratories and reduce the number of animals used in research.Graphical abstract (Source: Journal of Neuroscience Methods)
Source: Journal of Neuroscience Methods - January 1, 2020 Category: Neuroscience Source Type: research

Editorial Board
Publication date: 1 February 2020Source: Journal of Neuroscience Methods, Volume 331Author(s): (Source: Journal of Neuroscience Methods)
Source: Journal of Neuroscience Methods - December 31, 2019 Category: Neuroscience Source Type: research

Comparing SPM12 unified segmentation with CAT12 segmentation pipelines: a brain tissue volume-based age and Alzheimer’s disease study
ConclusionsOur findings indicate that other studies should take these pipeline effects on age and AD diagnosis, into account, for improved comparability in previous literature. Additionally, we encourage future studies to use CAT12 as this is a more advanced and computationally efficient brain segmentation tool. (Source: Journal of Neuroscience Methods)
Source: Journal of Neuroscience Methods - December 28, 2019 Category: Neuroscience Source Type: research