Quantification of human embryonic {zeta}-globin chains in Southeast Asian deletion (--SEA) carriers
Conclusion Our study has reported reliable approaches for the quantification of -globin and presented the expression patterns of -globin among the --SEA/αα carrier population, which might lay a foundation on subsequent genotype–phenotype studies on mechanisms of delayed haemoglobin switch in α-thalassaemia. (Source: Journal of Clinical Pathology)
Source: Journal of Clinical Pathology - October 18, 2023 Category: Pathology Authors: Ye, Y., Sun, G., Ren, Z., Liang, Y., Luo, H., Lin, P., Wang, X., Dong, Z., Huang, L., Qin, L., Yu, W., Wang, G., Zhou, Y., Tang, J., Lou, J., Liu, Y., Zeng, X., Chen, Y., Li, Y., Zhang, Q., Huang, J., Zhu, P., Lin, L., Zhang, X., Xu, X. Tags: Original research Source Type: research

Performance evaluation and clinical impact of the Oncomine Myeloid Research Assay for gene expression analysis in myeloid haematologic malignancies
Conclusions OMA gene expression analysis offers reproducible and accurate gene expression data for most targeted genes and demonstrates the utility of BAALC expression as a prognostic marker in AML. (Source: Journal of Clinical Pathology)
Source: Journal of Clinical Pathology - October 18, 2023 Category: Pathology Authors: Jeon, M. J., Yu, E. S., Kim, D. S., Choi, C. W., Kim, H. N., Ah Kwon, J., Yoon, S.-Y., Yoon, J. Tags: Original research Source Type: research

Increasing test specificity without impairing sensitivity: lessons learned from SARS-CoV-2 serology
Conclusion For SARS-CoV-2 serology, SIT² proved to be the best diagnostic choice at both 5% and 20% seroprevalence in all tested scenarios. It is an easy to apply algorithm and can potentially be helpful for the serology of other infectious diseases. (Source: Journal of Clinical Pathology)
Source: Journal of Clinical Pathology - October 18, 2023 Category: Pathology Authors: Perkmann, T., Koller, T., Perkmann-Nagele, N., Ozsvar-Kozma, M., Eyre, D., Matthews, P., Bown, A., Stoesser, N., Breyer, M.-K., Breyer-Kohansal, R., Burghuber, O. C., Hartl, S., Aletaha, D., Sieghart, D., Quehenberger, P., Marculescu, R., Mucher, P., Rada Tags: COVID-19 Original research Source Type: research

IGH cytogenetic abnormalities can be detected in multiple myeloma by imaging flow cytometry
Conclusions This automated high-throughput immuno-flowFISH method was able to identify translocations and trisomy involving the IGH gene in plasma cells in multiple myeloma. Thousands of cells were analysed and without prior cell isolation. The inclusion of positive plasma cell identification based on immunophenotype led to a lowest detection level of 0.05% marrow cells. This imaging flow cytometric FISH method offers the prospect of increased precision of detection of critical genetic lesions involving IGH and other chromosomal defects in multiple myeloma. (Source: Journal of Clinical Pathology)
Source: Journal of Clinical Pathology - October 18, 2023 Category: Pathology Authors: Hui, H., Fuller, K. A., Eresta Jaya, L., Konishi, Y., Ng, T. F., Frodsham, R., Speight, G., Yamada, K., Clarke, S. E., Erber, W. N. Tags: Original research Source Type: research

Comparison of two pathological processing methods for large endoscopic submucosal dissection (ESD) specimens
Conclusions The optimised technique not only reduced the workload and storage space, but also facilitated accurate pathological assessment. (Source: Journal of Clinical Pathology)
Source: Journal of Clinical Pathology - October 18, 2023 Category: Pathology Authors: Yu, Z., Jiang, D., Huang, W., Luo, R., Wang, H., Su, J., Liu, J., Xu, C., Hou, Y. Tags: Original research Source Type: research

Proposal to distinguish HER2-low from HER2-normal breast cancer by in situ hybridisation
Conclusions Current HER2 testing algorithms are unsatisfactory in detecting HER2-low cases. Using ISH to detect tumour with HER2 signals and HER2:CEP17 ratio higher than the upper bound of the benign glands can be an alternative method. (Source: Journal of Clinical Pathology)
Source: Journal of Clinical Pathology - October 18, 2023 Category: Pathology Authors: Chen, Y.-Y., Yang, C.-F., Hsu, C.-Y. Tags: Original research Source Type: research

Establishment and validation of prognostic nomograms integrating histopathological features in patients with endocervical adenocarcinoma
Conclusions A nomogram with five histological signatures had better performance in OS prediction compared with traditional staging systems in ECAs, which might enable a step forward to precision medicine. (Source: Journal of Clinical Pathology)
Source: Journal of Clinical Pathology - October 18, 2023 Category: Pathology Authors: Luo, R.-Z., Yang, X., Zhang, S.-W., Liu, L.-L. Tags: Original research Source Type: research

Comprehensive characterisation of acinar cystic transformation of the pancreas: a systematic review
Conclusions Globally considered, our findings demonstrated that ACT is a benign entity, without the need of surgical resection with the exception of symptomatic lesions. The rare occurrence of intracystic PanINs and driver mutations suggest considering follow-up if a preoperative diagnosis of ACT can be made. (Source: Journal of Clinical Pathology)
Source: Journal of Clinical Pathology - October 18, 2023 Category: Pathology Authors: Mattiolo, P., Wang, H., Basturk, O., Brosens, L. A. A., Hong, S.-M., Adsay, V., Scarpa, A., Luchini, C. Tags: Systematic review Source Type: research

Co-occurring IPMN and pancreatic cancer: the same or different? An overview from histology to molecular pathology
Intraductal papillary mucinous neoplasm (IPMN) of the pancreas is one of the most well-established precursors of pancreatic cancer. Its progression to acquire invasiveness is a complex process, based on the accumulation of morphological and genetic alterations. Recent advances in DNA sequencing also showed that co-occurring IPMNs and pancreatic cancers could be totally independent, further complicating our understanding of this complex scenario. The distinction between IPMN and related pancreatic cancer vs IPMN and co-occurring—but not related—pancreatic cancer is a challenging task in routine diagnostic activi...
Source: Journal of Clinical Pathology - October 18, 2023 Category: Pathology Authors: Omori, Y., Furukawa, T., Scarpa, A., Luchini, C. Tags: Review Source Type: research

Screening for and diagnosis of monoclonal gammopathy
This article provides an overview on initial laboratory testing of monoclonal gammopathy to guide clinicians and laboratory professionals in the selection and interpretation of appropriate investigations. (Source: Journal of Clinical Pathology)
Source: Journal of Clinical Pathology - October 18, 2023 Category: Pathology Authors: Chong, Y. P., Lim, S. M., Loh, T. P., Mollee, P., Wijeratne, N., Choy, K. W. Tags: Best practice Source Type: research

New kids on the block: FOS and FOSB gene
FOS and FOSB proto-oncogens are involved in a wide variety of tumourigenic processes. FOS and FOSB gene rearrangements are observed in epithelioid haemangioma, pseudomyogenic haemangioendothelioma, osteoid osteoma/osteoblastoma/cementoblastoma and proliferative myositis/fasciitis. In this review, we provide an overview of FOS and FOSB, including their functions and the differences between lesions with known FOS/FOSB gene rearrangements. Additionally, we discuss the use of FOS/FOSB immunohistochemistry as a diagnostic tool for these lesions. (Source: Journal of Clinical Pathology)
Source: Journal of Clinical Pathology - October 18, 2023 Category: Pathology Authors: Cordier, F., Creytens, D. Tags: Editor's choice, Gene of the month Source Type: research

Immunohistochemical enumeration of plasma cells: manual or digital?
Plasma cell enumeration in bone marrow samples is a critical component in the assessment and diagnosis of plasma cell dyscrasias, including multiple myeloma. This can be performed by morphological assessment or flow cytometric analysis of aspirated bone marrow. However, due to the patchy nature of the disease, the bone marrow trephine biopsy gives a better assessment of the overall plasma cell burden. Because of the challenges in cytological identification of plasma cells in H&E-stained sections, this is often aided by immunohistochemistry. CD138, or syndecan-1, is most commonly used for this purpose because of its hig...
Source: Journal of Clinical Pathology - September 19, 2023 Category: Pathology Authors: Malherbe, J. A., Fuller, K. A., Erber, W. N. Tags: PostScript Source Type: research

Impact of the transition to digital pathology in a clinical setting on histopathologists in training: experiences and perceived challenges within a UK training region
Conclusions The survey evidences the need for provision of training and support for histopathologists in training during the transition to DP, and for consideration of their need to maintain competence and confidence with GS reporting. (Source: Journal of Clinical Pathology)
Source: Journal of Clinical Pathology - September 19, 2023 Category: Pathology Authors: Browning, L., Winter, L., Cooper, R. A., Ghosh, A., Dytor, T., Colling, R., Fryer, E., Rittscher, J., Verrill, C. Tags: Open access Original research Source Type: research

Automatic CD30 scoring method for whole slide images of primary cutaneous CD30+ lymphoproliferative diseases
Conclusions In this study, using a few immunohistochemical WSIs, our model can accurately identify CD30, CD3 and Ki-67 markers. In addition, the model could be applied to additional tumour identification tasks to aid pathologists in diagnosis and benefit clinical evaluation. (Source: Journal of Clinical Pathology)
Source: Journal of Clinical Pathology - September 19, 2023 Category: Pathology Authors: Zheng, T., Zheng, S., Wang, K., Quan, H., Bai, Q., Li, S., Qi, R., Zhao, Y., Cui, X., Gao, X. Tags: Original research Source Type: research

Idylla EGFR assay on extracted DNA: advantages, limits and place in molecular screening according to the latest guidelines for non-small-cell lung cancer (NSCLC) patients
Conclusion Idylla EGFR on extracted DNA is feasible and enables tumour material to be saved compared with tissue section use. It is not necessary to replace the analytical thresholds of the Biocartis algorithm. Due to both the limits of the mutational repertoire and the high increase of targetable genes in NSCLC, the use of Idylla EGFR should be restricted to clinical emergency situations accompanied by NGS. (Source: Journal of Clinical Pathology)
Source: Journal of Clinical Pathology - September 19, 2023 Category: Pathology Authors: Khalifa, E., Chapusot, C., Tournier, B., Sentis, J., Marion, E., Remond, A., Aubry, M., Pioche, C., Bergeron, A., Primois, C., Blanchard, L., Milliere, A., Boucheix, M., Leger, Y., Bairrao, M., Brouste, V., Martin, L., Soubeyran, I. Tags: Original research Source Type: research